##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939290_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 805704 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.827532195446466 33.0 31.0 34.0 30.0 34.0 2 31.817336143298284 33.0 31.0 34.0 30.0 34.0 3 31.86178919305353 33.0 31.0 34.0 30.0 34.0 4 35.312227567444125 37.0 35.0 37.0 33.0 37.0 5 35.399147826000615 37.0 35.0 37.0 33.0 37.0 6 35.55540744491773 37.0 35.0 37.0 33.0 37.0 7 35.52178467526536 37.0 35.0 37.0 33.0 37.0 8 35.54549189280431 37.0 35.0 37.0 33.0 37.0 9 37.06066744114464 39.0 37.0 39.0 33.0 39.0 10-11 37.00164080108824 39.0 37.0 39.0 33.0 39.0 12-13 36.908109553880834 39.0 37.0 39.0 33.0 39.0 14-15 37.79562035189102 40.0 37.0 41.0 32.5 41.0 16-17 37.79607709531044 40.0 37.5 41.0 32.5 41.0 18-19 37.982170871684886 40.0 38.0 41.0 33.0 41.0 20-21 37.910024028675544 40.0 37.5 41.0 33.0 41.0 22-23 37.888431111177304 40.0 38.0 41.0 33.0 41.0 24-25 37.90732949073109 40.0 38.0 41.0 33.0 41.0 26-27 37.57360891841172 40.0 36.5 41.0 32.0 41.0 28-29 37.350041082084736 39.5 36.5 41.0 31.5 41.0 30-31 37.24920876649489 39.0 36.0 41.0 32.0 41.0 32-33 37.02947174148322 39.0 35.0 41.0 31.0 41.0 34-35 36.741286502239035 39.0 35.0 41.0 31.0 41.0 36-37 36.31701778817035 39.0 35.0 41.0 30.0 41.0 38-39 35.730998604946734 38.5 35.0 40.0 28.5 41.0 40-41 35.50005336947564 38.0 35.0 40.0 29.5 41.0 42-43 35.48531656787108 38.0 35.0 40.0 29.0 41.0 44-45 35.431427050132555 39.0 35.0 40.5 27.5 41.0 46-47 35.02857997477982 38.5 35.0 40.5 24.5 41.0 48-49 34.74236071808009 38.5 35.0 40.0 23.5 41.0 50-51 33.503918933007654 37.5 33.0 39.0 21.5 40.5 52-53 33.41072403761183 37.5 33.0 39.5 21.0 40.5 54-55 33.97222367023125 38.0 34.0 40.0 21.5 41.0 56-57 34.05374057966698 38.0 34.0 40.0 20.5 41.0 58-59 33.954768128245604 38.0 34.0 40.0 19.5 41.0 60-61 33.5401667361711 38.0 33.5 40.0 15.5 41.0 62-63 32.9767724871665 37.0 33.0 40.0 10.5 41.0 64-65 32.27654634952786 36.0 31.5 39.0 8.0 41.0 66-67 31.884563065344096 36.0 31.0 39.0 4.0 40.5 68-69 31.59053759196926 36.0 31.0 39.0 2.0 40.0 70-71 30.99101717255965 35.0 30.5 37.5 2.0 39.5 72-73 30.314289118584494 35.0 30.0 37.0 2.0 39.0 74-75 29.840618887333314 35.0 29.5 37.0 2.0 39.0 76-77 29.195681664730472 34.0 29.0 36.0 2.0 38.0 78-79 28.745945781577355 34.0 28.5 35.0 2.0 37.0 80-81 28.391650035248677 34.0 28.5 35.0 2.0 37.0 82-83 27.973261272129715 34.0 27.5 35.0 2.0 36.0 84-85 27.55596596268605 34.0 26.0 35.0 2.0 36.0 86-87 27.029525731534164 33.5 26.0 35.0 2.0 35.5 88-89 26.312240599525385 33.0 24.0 35.0 2.0 35.0 90-91 25.67718976199696 32.5 21.5 35.0 2.0 35.0 92-93 25.330519024356338 32.0 19.5 35.0 2.0 35.0 94-95 24.901464433588515 32.0 14.5 35.0 2.0 35.0 96-97 24.053892000039717 32.0 2.0 35.0 2.0 35.0 98-99 23.128866184107316 31.0 2.0 34.0 2.0 35.0 100-101 20.83478113550386 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6563120454519371 1101 2 0.3318914654791634 1101 3 0.7074762967462718 1101 4 0.6887565822334807 1101 5 0.6168065419957216 1101 6 0.6185660808119806 1101 7 0.6597765703174403 1101 8 0.5905390139578586 1101 9 0.702177623204669 1101 10-11 0.8072685364108594 1101 12-13 0.8719834552527175 1101 14-15 0.8637735324690752 1101 16-17 1.0339994287437904 1101 18-19 0.911988239918351 1101 20-21 0.9829582411789914 1101 22-23 1.00808372827764 1101 24-25 0.8560957978787158 1101 26-27 0.837005002628608 1101 28-29 0.9755189546777814 1101 30-31 0.8906942646384195 1101 32-33 0.9327738682547917 1101 34-35 1.0249255486772313 1101 36-37 0.7253876218501887 1101 38-39 0.8236877020809388 1101 40-41 1.0064196380852692 1101 42-43 1.0643087258851693 1101 44-45 0.9040892347273299 1101 46-47 0.6548523190060109 1101 48-49 0.7921520688913617 1101 50-51 0.6548605784667174 1101 52-53 0.6392956026790202 1101 54-55 0.7685630599285815 1101 56-57 0.6148337042270882 1101 58-59 0.4667739009656202 1101 60-61 0.7923489785883007 1101 62-63 0.9277416623807468 1101 64-65 0.754959008289191 1101 66-67 0.8927872974757349 1101 68-69 0.7683865219229524 1101 70-71 0.7719882736686436 1101 72-73 1.0477961954491377 1101 74-75 0.9656379161345399 1101 76-77 0.5479153176661917 1101 78-79 0.7828652037336106 1101 80-81 0.8123929493684834 1101 82-83 0.8652667541932217 1101 84-85 0.8125113783291233 1101 86-87 0.5495668264112901 1101 88-89 0.5616398618385752 1101 90-91 0.982715241486094 1101 92-93 1.2321787571609732 1101 94-95 1.272130663195714 1101 96-97 1.145076975240606 1101 98-99 1.30519099826817 1101 100-101 1.3897323804892423 1102 1 0.3429658411756229 1102 2 0.18451228543394294 1102 3 0.4494104484513315 1102 4 0.2869271187866147 1102 5 0.22811576468023986 1102 6 0.29805482217830104 1102 7 0.3771760892057259 1102 8 0.395763102581526 1102 9 0.11639209991824373 1102 10-11 0.4850940353319757 1102 12-13 0.4812670530598382 1102 14-15 0.5607774247248258 1102 16-17 0.5935932713397278 1102 18-19 0.6350991537208941 1102 20-21 0.6325767588180327 1102 22-23 0.6741240549380478 1102 24-25 0.7812560824160357 1102 26-27 0.7948994677789827 1102 28-29 0.9036350822446622 1102 30-31 0.8157595657462267 1102 32-33 0.8507009581483445 1102 34-35 0.9362626469334572 1102 36-37 0.9171839443395768 1102 38-39 0.9204768491916795 1102 40-41 0.7622027867859629 1102 42-43 0.9964554546352105 1102 44-45 0.760033442286769 1102 46-47 1.0253682235683144 1102 48-49 1.0824203970797583 1102 50-51 1.0770226904026217 1102 52-53 1.0276405164196163 1102 54-55 1.163248117589717 1102 56-57 1.0606456576852068 1102 58-59 0.8923409623641447 1102 60-61 1.005610523720911 1102 62-63 1.0631611897349416 1102 64-65 1.2156348360388023 1102 66-67 1.2755343834460646 1102 68-69 1.1818948174865973 1102 70-71 1.142920246949398 1102 72-73 1.0962016095046216 1102 74-75 0.9944777761372237 1102 76-77 1.1546168115903477 1102 78-79 1.3473981846646552 1102 80-81 1.0658386797600805 1102 82-83 0.7666646624929143 1102 84-85 0.5517033997210454 1102 86-87 0.7030311405173642 1102 88-89 0.5081084398746896 1102 90-91 0.49429186317328444 1102 92-93 0.6196089391781356 1102 94-95 0.6844960603193648 1102 96-97 0.7617930264077657 1102 98-99 1.1208993991395353 1102 100-101 1.070253550437311 1103 1 0.3765900424948647 1103 2 0.419970609289944 1103 3 0.25082067570055955 1103 4 0.3573599055362635 1103 5 0.22875264150247432 1103 6 0.05228033447126279 1103 7 0.21388542110539532 1103 8 0.2876532620075096 1103 9 0.4143677414475775 1103 10-11 0.3049385381338183 1103 12-13 0.18902950627627035 1103 14-15 0.40233570515740524 1103 16-17 0.41842009460641094 1103 18-19 0.4261094719525218 1103 20-21 0.40853052321496364 1103 22-23 0.38409696699528695 1103 24-25 0.29353043571725124 1103 26-27 0.24703962203551555 1103 28-29 0.4293407128147422 1103 30-31 0.2649004023707775 1103 32-33 0.22882589353016414 1103 34-35 0.008396184287946085 1103 36-37 0.08673068654920968 1103 38-39 0.3918566800471197 1103 40-41 0.3053966826938179 1103 42-43 0.3555474916071475 1103 44-45 0.061172790108365405 1103 46-47 -0.16701200016569118 1103 48-49 0.05150912560067411 1103 50-51 -0.1727095318425782 1103 52-53 -0.11826837711030436 1103 54-55 -0.19413593154077802 1103 56-57 -0.14895195195892796 1103 58-59 -0.26902873636002766 1103 60-61 -0.36000846678796705 1103 62-63 -0.20747837520375612 1103 64-65 -0.08192207965772269 1103 66-67 -0.036540428902728905 1103 68-69 0.12952824111307493 1103 70-71 -0.07306784262691934 1103 72-73 -0.06483207466237317 1103 74-75 -0.3531391052341277 1103 76-77 -0.24193206043462823 1103 78-79 -0.46692844216274665 1103 80-81 -0.6198688118137063 1103 82-83 -0.5738938453956735 1103 84-85 -0.9283238949999095 1103 86-87 -0.9735388228828974 1103 88-89 -0.6961935803482184 1103 90-91 -0.3490881331047042 1103 92-93 -0.6082223711752484 1103 94-95 -0.825154277762973 1103 96-97 -0.8338729027500236 1103 98-99 -0.7598836698623437 1103 100-101 -0.716039123411452 1104 1 0.2418343037103874 1104 2 0.29932267509648725 1104 3 0.2493984001375935 1104 4 0.39483784078181117 1104 5 0.4439331925660639 1104 6 0.2498655833305179 1104 7 0.13155514923644773 1104 8 0.30270795142538987 1104 9 0.3842622592571914 1104 10-11 0.5306517216541238 1104 12-13 0.5189019690730561 1104 14-15 0.5185219803930536 1104 16-17 0.5896805814532797 1104 18-19 0.4492760028860445 1104 20-21 0.6073315254321514 1104 22-23 0.46639606570120407 1104 24-25 0.5774478112486534 1104 26-27 0.6049708540378802 1104 28-29 0.4693797182349755 1104 30-31 0.6076259499468648 1104 32-33 0.5163301311320083 1104 34-35 0.38998005373473177 1104 36-37 0.44180567748951205 1104 38-39 0.8009284601516313 1104 40-41 0.7477458143263931 1104 42-43 0.550152780913578 1104 44-45 0.622081254011789 1104 46-47 0.862175822038175 1104 48-49 0.7101415589388083 1104 50-51 0.7013391996045257 1104 52-53 0.8281585939103522 1104 54-55 0.7352890015343405 1104 56-57 0.6680021572484591 1104 58-59 0.785939291664647 1104 60-61 0.846389045593618 1104 62-63 0.9020290498540149 1104 64-65 0.9554555169693373 1104 66-67 0.8007756007273628 1104 68-69 0.9275047131682861 1104 70-71 0.852032788239967 1104 72-73 0.7552393508474076 1104 74-75 0.792503860736467 1104 76-77 0.5529290436408729 1104 78-79 0.6131076797399047 1104 80-81 0.595798819922134 1104 82-83 0.8046720722058609 1104 84-85 0.7534935783372951 1104 86-87 0.8937920919325357 1104 88-89 0.6580748049988365 1104 90-91 0.8768880739786162 1104 92-93 0.9886628453418496 1104 94-95 0.7641022812653624 1104 96-97 0.7135395298867202 1104 98-99 0.8297498896063082 1104 100-101 1.0905489349291244 1105 1 0.21407035925678386 1105 2 0.2543416416638671 1105 3 0.1399921305253855 1105 4 0.1877847584600616 1105 5 0.43212004796658476 1105 6 0.1080930377376319 1105 7 0.2705026111968607 1105 8 0.2165743727331133 1105 9 0.41008604142584204 1105 10-11 0.42129173453917446 1105 12-13 0.2656006385438232 1105 14-15 0.41344219862428844 1105 16-17 0.2245301911067017 1105 18-19 0.24387890831608416 1105 20-21 0.2833012825971437 1105 22-23 0.33412346549496874 1105 24-25 0.29440164538329583 1105 26-27 0.28325010781592397 1105 28-29 0.25636174766694353 1105 30-31 0.4010264360982134 1105 32-33 0.3063158946358513 1105 34-35 0.5620861480288397 1105 36-37 0.5424867707191225 1105 38-39 0.6068872391082749 1105 40-41 0.748276211744944 1105 42-43 0.6334547964237771 1105 44-45 0.8072714102800163 1105 46-47 0.6676430808739653 1105 48-49 0.6604980267326752 1105 50-51 0.5620233289659566 1105 52-53 0.8455487794291159 1105 54-55 0.8613727062075753 1105 56-57 0.7654073753223258 1105 58-59 0.9562218555668878 1105 60-61 1.1359786309209596 1105 62-63 0.9496157878821236 1105 64-65 0.8534377852189934 1105 66-67 0.7592657545888954 1105 68-69 0.8696275247004088 1105 70-71 0.7591299314991069 1105 72-73 0.8964639116727895 1105 74-75 0.8788771827645299 1105 76-77 0.9850094789185029 1105 78-79 0.8780944151761929 1105 80-81 1.1917535445320162 1105 82-83 0.7901736171156202 1105 84-85 0.7776113876571067 1105 86-87 0.41329923887576214 1105 88-89 0.48168665405865596 1105 90-91 0.7177281198168011 1105 92-93 0.8817549049314906 1105 94-95 0.798502210175446 1105 96-97 0.7978227343387232 1105 98-99 0.6604235220973642 1105 100-101 0.46010727031431387 1106 1 0.31605656339414523 1106 2 0.21581254523847448 1106 3 0.24193797535965444 1106 4 0.4722809953937386 1106 5 0.3025473827961349 1106 6 0.3289496727193182 1106 7 0.39113130385374717 1106 8 0.32748875661325627 1106 9 0.33402454788596714 1106 10-11 0.4040074243059877 1106 12-13 0.4403397507444424 1106 14-15 0.6043294189387041 1106 16-17 0.42375248488382766 1106 18-19 0.46782215260744664 1106 20-21 0.6214955722821855 1106 22-23 0.6574699185263881 1106 24-25 0.5174631266399032 1106 26-27 0.5833329321248542 1106 28-29 0.40134727973416773 1106 30-31 0.5479667622364772 1106 32-33 0.45459421940892497 1106 34-35 0.3973947475434798 1106 36-37 0.4229205805846945 1106 38-39 0.480613508276754 1106 40-41 0.5622636933419756 1106 42-43 0.5201442982671409 1106 44-45 0.8225254720929129 1106 46-47 0.7313484860375041 1106 48-49 0.7975433046064779 1106 50-51 0.8157078701245482 1106 52-53 0.8406126611868814 1106 54-55 0.9099310894489889 1106 56-57 1.0306881971079278 1106 58-59 1.0542572348829253 1106 60-61 1.133106978769014 1106 62-63 1.0586683795635423 1106 64-65 0.7683656385778477 1106 66-67 0.6806914557440464 1106 68-69 0.8244970500925497 1106 70-71 0.8952960784666324 1106 72-73 0.8241110871357371 1106 74-75 0.9472475969387162 1106 76-77 0.9898122359853296 1106 78-79 1.0778571726641601 1106 80-81 1.135219812051023 1106 82-83 1.0449838318797546 1106 84-85 0.8252651960837802 1106 86-87 0.622911421068185 1106 88-89 0.6200813364572646 1106 90-91 0.610734729968744 1106 92-93 0.6811902256387974 1106 94-95 0.47876703739376225 1106 96-97 0.6924261690908935 1106 98-99 0.9747645862844472 1106 100-101 0.6022237823580561 1107 1 0.17612860737322578 1107 2 0.22920150207928813 1107 3 0.0741282601753106 1107 4 0.01176909335204357 1107 5 0.17177781600088338 1107 6 0.024550420041194343 1107 7 0.10841245010571754 1107 8 0.0773644269851701 1107 9 0.3083252777344825 1107 10-11 0.19773701072516303 1107 12-13 0.19919823674559467 1107 14-15 0.013879614505846405 1107 16-17 0.050535102929764264 1107 18-19 0.05902213513408583 1107 20-21 0.03343743305988767 1107 22-23 0.12843306991283043 1107 24-25 -0.19569666045400425 1107 26-27 0.11802761888139912 1107 28-29 3.8122353018366084E-5 1107 30-31 0.03943422147652598 1107 32-33 0.132028559924251 1107 34-35 0.15921866318750233 1107 36-37 0.19901384124679566 1107 38-39 0.39677585588894715 1107 40-41 -0.016605814312697476 1107 42-43 -0.12113616249174441 1107 44-45 0.07139810478959419 1107 46-47 0.20871629340050646 1107 48-49 0.25887931046677437 1107 50-51 -0.2816724710090668 1107 52-53 0.044542653525489584 1107 54-55 0.2858551610090956 1107 56-57 0.11748119835794313 1107 58-59 0.014312141534887246 1107 60-61 -0.1645574723660772 1107 62-63 0.0013356197146237037 1107 64-65 0.14777514004963876 1107 66-67 0.009115728919759647 1107 68-69 0.02204926853634248 1107 70-71 -0.2653008381585913 1107 72-73 -0.11963479654549403 1107 74-75 -0.14015613160601958 1107 76-77 -0.21094399057685465 1107 78-79 0.12493693852123045 1107 80-81 -0.20667994466613493 1107 82-83 -0.20010766800599455 1107 84-85 -0.20933204312642317 1107 86-87 -0.30601487498418223 1107 88-89 -0.42985717078073904 1107 90-91 -0.30984972009106926 1107 92-93 -0.2725885549899729 1107 94-95 -0.00475256636435617 1107 96-97 0.08008258920406774 1107 98-99 0.05847370871888913 1107 100-101 0.25964609314180365 1108 1 0.1645559370679912 1108 2 0.2815943664415279 1108 3 0.25217808835400746 1108 4 0.07637895791642535 1108 5 0.24707597650219526 1108 6 0.2447343699097999 1108 7 0.07780046304284838 1108 8 0.21587008698112697 1108 9 0.34570904567465277 1108 10-11 0.39158555529762396 1108 12-13 0.31688319732776904 1108 14-15 0.3545658890156531 1108 16-17 0.3413426630672163 1108 18-19 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-1.6728567413848303 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2652.0 3 617.0 4 594.0 5 604.0 6 731.0 7 835.0 8 1178.0 9 1405.0 10 1894.0 11 2811.0 12 3971.0 13 6049.0 14 5857.0 15 6879.0 16 8860.0 17 11547.0 18 9561.0 19 6700.0 20 6532.0 21 6883.0 22 7521.0 23 8347.0 24 9202.0 25 10387.0 26 11987.0 27 13821.0 28 15937.0 29 18518.0 30 21724.0 31 26506.0 32 33058.0 33 40935.0 34 54866.0 35 83888.0 36 109028.0 37 137696.0 38 99819.0 39 16297.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.06928482350218 16.241010820619664 13.126131037280981 18.56357331859718 2 12.139657689559842 23.990184201534497 40.255210760103125 23.614947348802534 3 25.799348616773678 29.75834701254677 29.672854517371256 14.769449853308297 4 11.13669547496412 14.972444687447576 40.62889486731822 33.261964970270085 5 11.684615786111781 42.81852955577482 32.60157428648833 12.895280371625065 6 24.385222236433787 36.869280794147706 19.868221549725504 18.877275419693003 7 24.415227010520017 39.218133520112964 19.058104112256544 17.30853535711048 8 20.941869332054956 30.316846045509983 21.673625721261917 27.067658901173147 9 25.975675940840354 23.031602896632066 22.176671704683617 28.816049457843967 10-11 22.214998761248886 24.764218161767857 27.307042005149977 25.713741071833283 12-13 34.19147119549187 22.56634118322803 24.600462610801497 18.6417250104786 14-15 28.650960512737083 23.84752752882035 30.73553828825193 16.765973670190636 16-17 18.368415202757454 28.361578693438133 33.58800003228869 19.682006071515723 18-19 17.203457768024524 33.482550641050715 32.854881666266145 16.45910992465861 20-21 19.569071567007406 30.11133694375773 27.82287185849918 22.49671963073568 22-23 17.94683890199975 28.868401440814807 25.887964228046208 27.296795429139237 24-25 20.90087952643082 28.72972682571141 29.47366917359545 20.895724474262316 26-27 25.0531348637996 23.54566989678607 32.23767650029129 19.163518739123038 28-29 19.340454324980794 25.481983568184457 35.81034040526181 19.36722170157294 30-31 18.366602969643413 32.93262378534207 31.76080142737639 16.93997181763813 32-33 17.765908626579403 31.51315532090488 29.134274378592806 21.58666167392291 34-35 19.827918117735454 26.615716762153678 29.791201593806893 23.765163526303972 36-37 23.446030020065688 26.665757420630914 32.11992581548782 17.76828674381557 38-39 26.490441846681044 22.691275134357053 33.50465206628447 17.31363095267743 40-41 19.573660421098108 26.707664687730908 36.44983033691708 17.268844554253903 42-43 17.224896767798526 32.03504415698268 32.007274819312514 18.732784255906274 44-45 18.352952019065093 32.8577646841239 27.627879519287713 21.161403777523294 46-47 18.1420580582725 29.69891005289687 24.98900836111862 27.170023527712004 48-49 21.107622016917883 28.290187451966137 27.58604367858758 23.0161468525284 50-51 25.022684428922226 29.692158131488107 28.10041806608471 17.18473937350496 52-53 19.071397387935377 29.83590365092424 32.280253571177234 18.812445389963152 54-55 15.781149299317843 28.820655879596625 33.79034643369751 21.60784838738803 56-57 20.35977874136584 33.193090803821285 26.110075236651486 20.33705521816139 58-59 18.696565036361452 29.462517794796046 28.231899105451635 23.60901806339087 60-61 19.195021397488052 25.93594566444209 31.02417997035835 23.844852967711507 62-63 26.957477343976187 24.84227173530666 28.20249239610606 19.99775852461109 64-65 23.738001579444305 26.829066259432444 31.82505536774396 17.607876793379287 66-67 18.59175421594298 27.80130694855167 33.37333984399329 20.233598991512057 68-69 17.554513505196894 33.20293861523301 30.571726853795546 18.67082102577455 70-71 18.453333466218098 31.741664543624925 26.308878591703877 23.496123398453097 72-73 18.09824834565979 29.080389256520046 25.52825223822499 27.293110159595173 74-75 19.990993869478373 29.646736710196297 28.240758906633335 22.121510513691998 76-77 23.143234339874084 25.188220421837926 30.35109569506112 21.317449543226868 78-79 19.363834352123146 25.502598131935045 33.94923525390467 21.184332262037138 80-81 18.15969896125695 32.41713838866572 30.445743046199286 18.97741960387804 82-83 17.765871926163793 30.802530367082564 27.95820249039032 23.473395216363322 84-85 19.149585391430843 26.72948690165719 28.19070499647539 25.930222710436578 86-87 21.954184099245992 26.710935403303594 30.13364683436398 21.201233663086434 88-89 24.485937307331582 22.66216530965824 31.67584001873282 21.17605736427736 90-91 19.42312543798178 25.230491318227827 34.37801136806043 20.968371875729968 92-93 17.44159248158142 29.918452465486183 31.838170624450306 20.80178442848209 94-95 17.49365603172171 31.20204651371381 28.496470174940296 22.80782727962418 96-97 17.00916847152068 27.837420494545572 26.44766733130143 28.705743702632315 98-99 19.95327417136707 26.567077910429916 27.7221438821677 25.757504036035318 100-101 23.359535105870897 26.489923536822076 29.018831525072684 21.131709832234343 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3461.0 1 2209.5 2 1549.5 3 2005.5 4 1509.5 5 1446.5 6 2752.5 7 5210.0 8 6930.5 9 6498.0 10 4773.0 11 3376.0 12 3013.0 13 2912.5 14 2645.5 15 2513.0 16 2342.5 17 2115.0 18 2045.0 19 2044.5 20 2123.0 21 2198.0 22 2441.5 23 2705.0 24 3029.0 25 3557.5 26 4295.5 27 5314.0 28 6195.0 29 7069.5 30 8174.5 31 9327.5 32 10599.0 33 11827.0 34 12892.0 35 13632.5 36 14333.0 37 14938.0 38 14891.0 39 14608.5 40 14395.5 41 14320.5 42 14299.5 43 14146.5 44 15929.5 45 17998.5 46 24645.5 47 43711.0 48 140221.0 49 142510.5 50 48849.5 51 27133.0 52 13749.5 53 8705.0 54 6611.0 55 5387.5 56 4665.0 57 4098.0 58 3662.0 59 3334.0 60 3149.5 61 3025.0 62 2466.0 63 1398.0 64 630.5 65 471.5 66 373.0 67 308.0 68 253.0 69 207.5 70 194.0 71 160.0 72 136.0 73 170.0 74 160.0 75 124.0 76 101.0 77 65.5 78 45.5 79 36.5 80 26.0 81 13.0 82 11.0 83 12.0 84 7.0 85 3.0 86 2.5 87 1.5 88 0.5 89 0.5 90 1.5 91 1.5 92 1.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27776950344046947 2 0.10909713741026482 3 0.11852988194175529 4 0.11902634218025478 5 0.12684559093662187 6 0.051259519625073226 7 0.05920288344106521 8 0.06429160088568507 9 0.061188724395063204 10-11 0.05678263977838015 12-13 0.06100255180562589 14-15 0.056720582248567714 16-17 0.05814790543425377 18-19 0.06236781746149951 20-21 0.06646361442912037 22-23 0.07496549601342428 24-25 0.08297091735922871 26-27 0.08402589536604013 28-29 0.07688927943760984 30-31 0.07328994270848847 32-33 0.06646361442912037 34-35 0.22868199735883152 36-37 0.35261088439426885 38-39 0.3251814562171716 40-41 0.3096050162342498 42-43 0.33014605860216656 44-45 0.3438607726907152 46-47 0.35012858320177137 48-49 0.35807194701776335 50-51 0.35254882686445643 52-53 0.3540382075799549 54-55 0.3396408606634695 56-57 0.31916187582536515 58-59 0.30401983855113046 60-61 0.26126220051036114 62-63 0.3303322311916039 64-65 0.3586925223158877 66-67 0.3621677439853842 68-69 0.3739586746497473 70-71 0.372779581583311 72-73 0.35887869490532504 74-75 0.3621056864555718 76-77 0.3461569012937754 78-79 0.3260502616345457 80-81 0.3249332260979218 82-83 0.33976497572309433 84-85 0.34479163563790177 86-87 0.3387720552460954 88-89 0.3518661940365196 90-91 0.37439307735843436 92-93 0.3701111078013762 94-95 0.3689940722647523 96-97 0.3732760418218105 98-99 0.36440181505863195 100-101 0.34361254257146545 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 805704.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.957099758210454 #Duplication Level Percentage of deduplicated Percentage of total 1 77.48072996484872 24.760594168256368 2 12.326045552237872 7.878093346742239 3 4.318223200502519 4.1399366879003345 4 1.9271008109880594 2.4633821144349475 5 1.0804153595805022 1.7263470713208466 6 0.6568742152511586 1.2595076895226491 7 0.41606292821833557 0.9307315151936554 8 0.29199227461482363 0.7464980998794162 9 0.2184298764856713 0.6282346817723568 >10 1.0909487111275649 6.273672515635349 >50 0.08490445393597586 1.8520140159575509 >100 0.07360982470312458 4.959738339454546 >500 0.010126219271329307 2.24618930994846 >1k 0.017526148738839186 11.307118093915209 >5k 0.004284169691716245 9.374451855488894 >10k+ 0.0027262898038194288 19.45349049457718 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 41372 5.134888246800314 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 34039 4.224752514571108 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 25337 3.144703265715449 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 15832 1.9649896239810154 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 14740 1.8294559788706521 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 13674 1.697149325310536 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 11305 1.4031207490592077 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 9774 1.2131005927735248 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9116 1.1314328835403573 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 8113 1.0069454787366081 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 6577 0.8163047471528005 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6415 0.7961981074935709 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 6199 0.769389254614598 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 6190 0.768272219077974 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 6178 0.7667828383624756 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5854 0.7265695590440162 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5510 0.6838739785330593 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 5393 0.6693525165569489 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 4786 0.59401467536465 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4213 0.5228967461995968 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 4060 0.503907142076991 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 3764 0.4671690844280282 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3683 0.45711576459841335 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 3471 0.4308033719579399 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 3327 0.4129308033719579 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 3277 0.4067250503907142 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2775 0.34441929045902714 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 2715 0.3369723868815347 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2653 0.32927725318479245 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 2460 0.3053230466771916 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2449 0.303957781021318 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 2299 0.2853405220775868 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2278 0.28273410582546443 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2136 0.26510976735873226 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 2100 0.26064162521223677 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 2079 0.2580352089601144 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 1984 0.2462442782957513 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1859 0.23072989584264195 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 1737 0.21558785856840723 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 1660 0.2060309989772919 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1576 0.19560533396880245 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1552 0.19262657253780543 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 1534 0.1903925014645577 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1519 0.18853077557018458 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1436 0.17822922562132 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 1411 0.17512634913069813 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1399 0.17363696841519963 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1365 0.16941705638795387 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1312 0.16283895822783553 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1295 0.16072900221421266 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1287 0.15973608173721368 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1270 0.1576261257235908 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 1190 0.14769692095360082 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1189 0.14757280589397595 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1156 0.1434770089263551 No Hit TCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTA 1133 0.14062236255498298 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 1132 0.1404982474953581 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1101 0.136650680646987 No Hit GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1094 0.13578187522961285 No Hit CATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1072 0.1330513439178656 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 1042 0.12932789212911938 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 1009 0.1252320951614985 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1008 0.12510798010187363 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 993 0.12324625420750053 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 910 0.11294470425863594 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 876 0.10872479223139019 No Hit TTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCG 869 0.10785598681401606 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 867 0.10760775669476633 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 836 0.1037601898463952 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 819 0.10165023383277233 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 813 0.10090554347502309 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 6.205752981243732E-5 0.0 14-15 0.0 0.0 0.0 3.102876490621866E-4 0.0 16-17 0.0 0.0 0.0 4.964602384994986E-4 0.0 18-19 0.0 0.0 0.0 6.826328279368105E-4 0.0 20-21 0.0 0.0 0.0 9.929204769989972E-4 0.0 22-23 0.0 0.0 0.0 0.002296128603060181 0.0 24-25 0.0 0.0 0.0 0.004344027086870613 0.0 26-27 0.0 0.0 0.0 0.007757191226554666 0.0 28-29 0.0 0.0 0.0 0.017748453526357073 0.0 30-31 0.0 0.0 0.0 0.041826775093582756 0.0 32-33 0.0 0.0 0.0 0.07813043003385858 0.0 34-35 0.0 0.0 0.0 0.14893807154984956 0.0 36-37 0.0 0.0 0.0 0.25561496529742933 0.0 38-39 0.0 0.0 0.0 0.3774338963192438 0.0 40-41 0.0 0.0 0.0 0.5063894432694885 0.0 42-43 0.0 0.0 0.0 0.6293254098279268 0.0 44-45 0.0 0.0 0.0 0.7575362664204224 0.0 46-47 0.0 0.0 0.0 0.9398612890093632 0.0 48-49 0.0 0.0 0.0 1.1641992592813242 0.0 50-51 0.0 0.0 0.0 1.372091984152989 0.0 52-53 0.0 0.0 0.0 1.5637876937436082 0.0 54-55 0.0 0.0 0.0 1.755359288274602 0.0 56-57 0.0 0.0 0.0 1.963376128205892 0.0 58-59 0.0 0.0 0.0 2.3033272765184236 0.0 60-61 0.0 0.0 0.0 2.8429795557673785 0.0 62-63 0.0 0.0 0.0 3.256965337146148 0.0 64-65 0.0 0.0 0.0 3.547332519138542 0.0 66-67 0.0 0.0 0.0 3.894668513498754 0.0 68-69 0.0 0.0 0.0 4.27446059595087 0.0 70-71 0.0 0.0 0.0 4.736727135523716 0.0 72-73 0.0 0.0 0.0 5.298223665266649 0.0 74-75 0.0 0.0 0.0 5.822423619592307 0.0 76-77 0.0 0.0 0.0 6.26706582069842 0.0 78-79 0.0 0.0 0.0 6.769980042298412 0.0 80-81 0.0 0.0 0.0 7.3058468122288085 0.0 82-83 0.0 0.0 0.0 7.877706949450419 0.0 84-85 0.0 0.0 0.0 8.85418218104912 0.0 86-87 0.0 0.0 0.0 9.953779551795698 0.0 88-89 0.0 0.0 0.0 10.667379087108912 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGTAC 25 0.0050117103 57.025894 1 CATTCCG 60 8.3976614E-5 39.49556 9 GTAAGCG 40 4.4694275E-4 29.71579 94-95 GCTTATG 185 2.8921932E-10 28.256071 1 CCCTTAG 85 6.388496E-4 27.953869 1 AGTACTC 480 0.0 26.681044 5 AAGTACT 500 0.0 26.56246 4 CCTAGTG 90 9.019257E-4 26.351648 2 CGGGAGT 210 1.2842065E-9 24.845839 4 TGTAGGG 115 1.2936292E-4 24.747635 2 GTCACTA 135 1.3373583E-5 24.640818 1 CATGGGG 1750 0.0 24.394096 4 CCGACGC 50 0.0016435949 23.775593 88-89 GTCGGGA 220 2.2027962E-9 23.716484 2 AAAGTAC 485 0.0 23.471983 3 TTAGGCA 145 2.3588253E-5 22.898674 4 ATGGGGA 705 0.0 22.875473 5 TATGTAC 275 5.2750693E-11 22.422857 4 TGGGATG 575 0.0 22.254889 6 GAGTACT 5625 0.0 21.864742 12-13 >>END_MODULE