##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939290_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 805704 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07298213736062 33.0 31.0 34.0 30.0 34.0 2 32.22649012540585 33.0 31.0 34.0 30.0 34.0 3 32.32401725695789 34.0 31.0 34.0 30.0 34.0 4 35.81574871168568 37.0 35.0 37.0 35.0 37.0 5 35.775420005361774 37.0 35.0 37.0 35.0 37.0 6 35.88435331089333 37.0 35.0 37.0 35.0 37.0 7 35.84820976437997 37.0 35.0 37.0 35.0 37.0 8 35.88453948348277 37.0 35.0 37.0 35.0 37.0 9 37.485587759276356 39.0 37.0 39.0 34.0 39.0 10-11 37.38379293139913 39.0 37.0 39.0 34.0 39.0 12-13 37.295580635071936 39.0 37.0 39.0 33.5 39.0 14-15 38.18567612919881 40.0 37.0 41.0 33.0 41.0 16-17 38.188353291034915 40.0 37.5 41.0 33.0 41.0 18-19 38.6121311300428 40.0 38.0 41.0 34.0 41.0 20-21 38.64945997537558 40.0 38.0 41.0 34.0 41.0 22-23 38.64815552609892 40.0 38.0 41.0 34.0 41.0 24-25 38.53393988362972 40.0 38.0 41.0 34.0 41.0 26-27 38.057853132167644 40.0 37.5 41.0 33.5 41.0 28-29 37.8019440141789 39.5 37.0 41.0 33.0 41.0 30-31 37.68661878307666 39.0 36.5 41.0 33.0 41.0 32-33 37.47694748940057 39.5 36.0 41.0 33.0 41.0 34-35 37.44185457686694 40.0 35.5 41.0 33.0 41.0 36-37 37.14722156027524 39.0 35.0 41.0 32.0 41.0 38-39 36.823883833268795 39.0 35.0 41.0 31.0 41.0 40-41 36.51423723848957 39.0 35.0 41.0 30.5 41.0 42-43 36.46828177593757 39.0 35.0 41.0 31.0 41.0 44-45 36.284412762006895 39.0 35.0 41.0 30.0 41.0 46-47 35.81060103958774 39.0 35.0 41.0 29.0 41.0 48-49 35.40537716084319 39.0 35.0 41.0 27.0 41.0 50-51 35.18694520568348 39.0 35.0 40.5 26.0 41.0 52-53 34.646355857734356 38.0 34.0 40.0 23.5 41.0 54-55 34.458589010356164 38.0 34.0 40.0 23.0 41.0 56-57 34.455395529872014 38.0 34.0 40.0 23.0 41.0 58-59 34.32215739278941 38.0 34.0 40.0 23.0 41.0 60-61 33.87677360420204 38.0 33.5 40.0 22.0 41.0 62-63 33.28510035943721 37.0 33.0 39.5 21.0 41.0 64-65 32.54835646838045 36.0 32.0 39.0 18.0 40.5 66-67 32.171291814363585 36.0 31.5 39.0 14.0 40.0 68-69 31.950640061362485 35.0 31.5 38.0 13.5 40.0 70-71 31.489119453297985 35.0 31.0 37.5 9.5 39.0 72-73 30.849478220289335 35.0 30.5 37.0 6.5 39.0 74-75 30.324588186232162 34.5 30.0 36.0 2.0 38.5 76-77 28.146061705043042 32.5 26.5 34.5 2.0 36.0 78-79 29.007306653560114 34.0 29.0 35.0 2.0 37.0 80-81 29.21837175935579 34.0 29.0 35.0 2.0 36.5 82-83 28.978227115665305 34.0 29.0 35.0 2.0 36.0 84-85 28.684124070378203 34.0 29.0 35.0 2.0 36.0 86-87 28.027013022152055 34.0 28.0 35.0 2.0 35.5 88-89 27.268308212445266 33.0 25.5 35.0 2.0 35.0 90-91 26.629898821403394 33.0 25.0 35.0 2.0 35.0 92-93 26.436441919116696 33.0 25.0 35.0 2.0 35.0 94-95 26.154924140875558 33.0 24.5 35.0 2.0 35.0 96-97 25.38509365722399 32.0 21.0 34.0 2.0 35.0 98-99 24.581365489062982 32.0 10.0 34.0 2.0 35.0 100-101 21.497556174475985 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3819237400685793 1101 2 0.49056627521432006 1101 3 0.40546576373091625 1101 4 0.17976136422937827 1101 5 0.3044712055927832 1101 6 0.3064705421020477 1101 7 0.42022816939905283 1101 8 0.28461680718238824 1101 9 0.5143863357499256 1101 10-11 0.4235240962528195 1101 12-13 0.4550858915770206 1101 14-15 0.5093623506928822 1101 16-17 0.560264922864782 1101 18-19 0.4972861162150153 1101 20-21 0.562547187486615 1101 22-23 0.5867662383569936 1101 24-25 0.49249912370707705 1101 26-27 0.808408302814783 1101 28-29 0.7059237787421395 1101 30-31 0.5297173218853715 1101 32-33 0.47161564662284405 1101 34-35 0.5835623904498277 1101 36-37 0.5793931122443183 1101 38-39 0.4987787509528516 1101 40-41 0.7476108977382268 1101 42-43 0.5161830660136886 1101 44-45 0.8462575004435138 1101 46-47 0.7034882886886678 1101 48-49 0.6000476856182075 1101 50-51 0.5042234202592866 1101 52-53 0.40332056888775014 1101 54-55 0.5952073967671012 1101 56-57 0.5589356975353823 1101 58-59 0.57926652519307 1101 60-61 0.7611331426428407 1101 62-63 0.7489825565353954 1101 64-65 0.9960197170040459 1101 66-67 0.9409641355447604 1101 68-69 1.0868738690150543 1101 70-71 0.9443901492821958 1101 72-73 0.7668037947160222 1101 74-75 0.7926566487359601 1101 76-77 0.8693824492653164 1101 78-79 0.9989171335019478 1101 80-81 1.133124916498307 1101 82-83 0.7539792235732143 1101 84-85 0.9460576217974541 1101 86-87 0.8655908867671833 1101 88-89 0.930282304699336 1101 90-91 0.9541406159753443 1101 92-93 0.7868623776481662 1101 94-95 1.0547826974301024 1101 96-97 1.2159026938390518 1101 98-99 1.1254496870762232 1101 100-101 0.8006462702282704 1102 1 0.2556721785203493 1102 2 0.2673366321248807 1102 3 0.20034491796541687 1102 4 0.08099098667025117 1102 5 0.18201278665391385 1102 6 0.25201612898607095 1102 7 0.2878894089328057 1102 8 0.354892155885139 1102 9 0.40854382240578957 1102 10-11 0.29569498383167314 1102 12-13 0.30290282134090063 1102 14-15 0.36478311696137666 1102 16-17 0.39302594662096624 1102 18-19 0.34058307364798424 1102 20-21 0.486830940158022 1102 22-23 0.48048081315128144 1102 24-25 0.2871532085107589 1102 26-27 0.4259389401329443 1102 28-29 0.4912736171670744 1102 30-31 0.5037692943401737 1102 32-33 0.4130170651797087 1102 34-35 0.6229949677012954 1102 36-37 0.7610654400610386 1102 38-39 0.7357510508940024 1102 40-41 0.724988322654859 1102 42-43 0.8605238087428049 1102 44-45 1.1958481425108474 1102 46-47 1.3202477018562533 1102 48-49 1.2145946043351046 1102 50-51 1.2062686612797435 1102 52-53 1.4659001949421153 1102 54-55 1.1505527957491282 1102 56-57 1.0258979829192896 1102 58-59 1.291045502882291 1102 60-61 1.3750678312107567 1102 62-63 1.082001687437014 1102 64-65 1.0956893398578842 1102 66-67 0.878149115773752 1102 68-69 1.129680422871683 1102 70-71 1.0492528879022522 1102 72-73 1.270454462181096 1102 74-75 1.2273730922259816 1102 76-77 1.3810003680957799 1102 78-79 1.2482445062809333 1102 80-81 1.1786880546682248 1102 82-83 1.3312430813458498 1102 84-85 1.3618868168128877 1102 86-87 1.4519617970594645 1102 88-89 1.5537225632194556 1102 90-91 1.8851227652851676 1102 92-93 1.840314302611258 1102 94-95 1.7291949874599375 1102 96-97 1.4059542120252821 1102 98-99 1.597581342368965 1102 100-101 1.3290834830840765 1103 1 0.12197767261682912 1103 2 0.10208363901059414 1103 3 0.11332645267313524 1103 4 0.06646895445420853 1103 5 -0.07216309887368055 1103 6 0.0036569139160818054 1103 7 0.06369636081552699 1103 8 -0.03947934180928314 1103 9 0.09515493466566483 1103 10-11 0.13646750714591604 1103 12-13 0.22248574973361457 1103 14-15 0.26463940611999703 1103 16-17 0.15487226907087148 1103 18-19 -0.07262384928578314 1103 20-21 0.08837350544823863 1103 22-23 -0.004226905878518039 1103 24-25 0.05703286360473925 1103 26-27 -0.040870058853840874 1103 28-29 -0.06526057360648707 1103 30-31 -0.0662313259943943 1103 32-33 0.009850257214615965 1103 34-35 0.12328755883878983 1103 36-37 0.2327182181260028 1103 38-39 0.36188884787917175 1103 40-41 0.27140072107507507 1103 42-43 0.38093798545544644 1103 44-45 0.6850077959506038 1103 46-47 0.784996156189294 1103 48-49 0.6206480358191584 1103 50-51 0.594482304504659 1103 52-53 0.45726513776794775 1103 54-55 0.6909717283601111 1103 56-57 0.4435847553185326 1103 58-59 0.38087408376357956 1103 60-61 0.5191839578417188 1103 62-63 0.586905432190008 1103 64-65 0.46213957868669553 1103 66-67 0.22585801343922185 1103 68-69 0.4481488168254977 1103 70-71 0.5102110980302506 1103 72-73 0.5616467452190825 1103 74-75 0.45649780015179076 1103 76-77 0.5763395877148909 1103 78-79 0.4218368637590686 1103 80-81 0.5685621267511003 1103 82-83 0.4243646048875469 1103 84-85 0.31245235515990544 1103 86-87 0.5396936471039204 1103 88-89 0.6501546055676286 1103 90-91 0.7808031689161901 1103 92-93 0.46714273874781753 1103 94-95 0.7832447382922219 1103 96-97 0.6290616746501208 1103 98-99 0.4884686275781256 1103 100-101 0.5773961730615369 1104 1 0.16381985068573357 1104 2 0.1774642438478935 1104 3 0.18814399225331613 1104 4 0.18758643441570655 1104 5 0.16702468436373152 1104 6 0.18230056959290408 1104 7 0.20321185192922542 1104 8 0.21891890314164186 1104 9 0.35145288775785133 1104 10-11 0.30968508413388207 1104 12-13 0.33309689959470035 1104 14-15 0.5402949192887121 1104 16-17 0.1622515412554435 1104 18-19 0.24711928385089266 1104 20-21 0.21224786201467083 1104 22-23 0.24677063533825105 1104 24-25 0.17445729790924247 1104 26-27 0.3335946394873517 1104 28-29 0.2755565918957288 1104 30-31 0.26877496708789295 1104 32-33 0.19875085768766354 1104 34-35 0.21778608006186317 1104 36-37 0.4068339718736027 1104 38-39 0.24690176525859897 1104 40-41 0.4134811790088335 1104 42-43 0.12506963751618372 1104 44-45 0.3446699178298829 1104 46-47 0.4553574921151977 1104 48-49 0.4571633048240642 1104 50-51 0.22230396283899978 1104 52-53 0.643076672617596 1104 54-55 0.4904035974640024 1104 56-57 0.5138948503577154 1104 58-59 0.5707400781290275 1104 60-61 0.569692275231283 1104 62-63 0.6926262921017141 1104 64-65 0.8583134625900399 1104 66-67 0.6621449390249907 1104 68-69 0.7674759830680777 1104 70-71 0.5827783249046341 1104 72-73 0.6076807395533628 1104 74-75 0.5210788523239742 1104 76-77 0.7046853448525745 1104 78-79 0.808921822318247 1104 80-81 0.9159675454261667 1104 82-83 0.770703316890387 1104 84-85 0.8126413424607364 1104 86-87 0.917405225206334 1104 88-89 1.192184796776015 1104 90-91 1.3138720499165402 1104 92-93 1.16367297087098 1104 94-95 0.775024764867343 1104 96-97 0.8599125851013163 1104 98-99 0.7666774176307669 1104 100-101 0.681377180811964 1105 1 0.1636050998984686 1105 2 0.05405844446585206 1105 3 0.17802663835327337 1105 4 0.07941127923049152 1105 5 0.20559836887630922 1105 6 0.1076968866169068 1105 7 0.20167157042056516 1105 8 0.23512518238060665 1105 9 0.22257649720022243 1105 10-11 0.12114222376447259 1105 12-13 0.2750928970843347 1105 14-15 0.2931824842924726 1105 16-17 0.21566154308823116 1105 18-19 0.28575406125985126 1105 20-21 0.2403376352489417 1105 22-23 0.2276568896216986 1105 24-25 0.30179988650782974 1105 26-27 0.25895855876327545 1105 28-29 0.28186119367186535 1105 30-31 0.2916699928302222 1105 32-33 0.35415822697201094 1105 34-35 0.6230578416292474 1105 36-37 0.7013165428107939 1105 38-39 0.636993793376142 1105 40-41 0.7319417861241178 1105 42-43 0.715226090869912 1105 44-45 0.9967588320610048 1105 46-47 0.9557036851946705 1105 48-49 0.7553929067390541 1105 50-51 0.9665117083074719 1105 52-53 0.9483338089158408 1105 54-55 0.9472312840241202 1105 56-57 0.9314249941481805 1105 58-59 0.8913019106197098 1105 60-61 0.6889668989082978 1105 62-63 0.7097526673861267 1105 64-65 0.8804040985300645 1105 66-67 0.7917544443391478 1105 68-69 0.7858455062287639 1105 70-71 0.8408837786489833 1105 72-73 0.6669001204416567 1105 74-75 0.6921361852302752 1105 76-77 0.943376216648204 1105 78-79 0.8583681910860754 1105 80-81 1.0334315978105444 1105 82-83 0.9935285486268519 1105 84-85 0.8501107265888024 1105 86-87 0.7195669880881432 1105 88-89 0.8531969764779959 1105 90-91 0.92728268119129 1105 92-93 0.9113500523337308 1105 94-95 0.9032467209137813 1105 96-97 0.9428896974807692 1105 98-99 1.2519780965024374 1105 100-101 1.3068703208202201 1106 1 0.1593884301024886 1106 2 -0.012530896115777068 1106 3 0.06911793636110275 1106 4 0.0599001883185295 1106 5 0.10529146269765732 1106 6 0.03422189797892372 1106 7 0.14993796287793515 1106 8 0.09258665240680841 1106 9 0.15146866942564685 1106 10-11 0.11676674888463623 1106 12-13 0.1147234515334219 1106 14-15 0.08363084105468488 1106 16-17 0.10906911414254239 1106 18-19 0.08970611440779663 1106 20-21 0.10296070918894173 1106 22-23 0.17277613381478574 1106 24-25 0.21851056821155623 1106 26-27 0.3311350306933747 1106 28-29 0.21162021353742233 1106 30-31 0.3163988397298425 1106 32-33 0.17550338499622598 1106 34-35 0.22729363822538318 1106 36-37 0.26276796863478324 1106 38-39 0.45791342396568524 1106 40-41 0.247953900934057 1106 42-43 0.5943859505599178 1106 44-45 0.6155371384091737 1106 46-47 0.4933536507137788 1106 48-49 0.18462286878376233 1106 50-51 0.06828295528710981 1106 52-53 -0.10071064071486546 1106 54-55 0.14008733642804572 1106 56-57 0.26788734330528285 1106 58-59 0.34380866202922533 1106 60-61 0.04727065004230724 1106 62-63 0.39642321790456236 1106 64-65 0.11683152811624353 1106 66-67 0.29284853240314845 1106 68-69 0.34944650362997365 1106 70-71 0.5836660437183561 1106 72-73 0.5151122910527128 1106 74-75 0.581690320216353 1106 76-77 0.611619235804838 1106 78-79 0.8784741430304308 1106 80-81 0.5723423528629148 1106 82-83 0.38041370535255226 1106 84-85 0.657946923970357 1106 86-87 0.659602714845132 1106 88-89 0.5635408903828285 1106 90-91 0.3753586830232507 1106 92-93 0.18689354299230487 1106 94-95 0.5219899322157886 1106 96-97 0.46764398828885234 1106 98-99 0.5602836901263686 1106 100-101 0.6225160433482557 1107 1 -0.004657793637072416 1107 2 -0.14017710103788517 1107 3 0.01406679320057691 1107 4 -0.0200756850130972 1107 5 0.11550665376204705 1107 6 0.12510853624019092 1107 7 0.041478843803751886 1107 8 0.030776345816661888 1107 9 0.05489983113481145 1107 10-11 -0.0672301798375301 1107 12-13 0.059316960854779666 1107 14-15 0.044714827398578905 1107 16-17 0.04969499621084594 1107 18-19 0.14367021338761532 1107 20-21 0.04501722204529557 1107 22-23 0.15009101707934036 1107 24-25 0.10242617863642067 1107 26-27 0.054859475914412315 1107 28-29 -0.03637707137232127 1107 30-31 0.02461736417237148 1107 32-33 -0.040704998180963514 1107 34-35 -0.03972607978158038 1107 36-37 0.02001268966597536 1107 38-39 -0.047671328517544964 1107 40-41 -0.018365202817335557 1107 42-43 -0.32488799801466683 1107 44-45 -0.38748205539719294 1107 46-47 -0.41645276469503045 1107 48-49 -0.5849078036715127 1107 50-51 -0.5716416404735867 1107 52-53 -0.38901928733645974 1107 54-55 -0.27665297594174376 1107 56-57 -0.3828094066510275 1107 58-59 -0.5554120936260958 1107 60-61 -0.5574073362209475 1107 62-63 -0.42315012985548606 1107 64-65 -0.27854592991410954 1107 66-67 -0.31846932805196815 1107 68-69 -0.2271052645389524 1107 70-71 -0.16437492457423275 1107 72-73 -0.293736786219835 1107 74-75 -0.43009831269538523 1107 76-77 -0.28939337861202574 1107 78-79 0.07351540702591208 1107 80-81 0.004402931634135854 1107 82-83 -0.09544534517231895 1107 84-85 -0.24151073003036316 1107 86-87 -0.04580327179447252 1107 88-89 0.11925232467629954 1107 90-91 0.06684731190267357 1107 92-93 0.10622125573037877 1107 94-95 0.5157364879490665 1107 96-97 0.6519509010161748 1107 98-99 0.8057271772700112 1107 100-101 0.991890127677344 1108 1 0.09257285119817737 1108 2 0.012983905632822257 1108 3 0.10802525287310516 1108 4 -0.007365961948615052 1108 5 -0.07724905676958826 1108 6 0.07573517864973667 1108 7 0.1292340489786099 1108 8 0.18718426981100578 1108 9 -0.05848959680130861 1108 10-11 0.07383384886673383 1108 12-13 0.07397230752953732 1108 14-15 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-0.48226047208556366 2316 66-67 -0.6233397271953116 2316 68-69 -0.44065272711270254 2316 70-71 -0.4936314387363687 2316 72-73 -0.44781824211669985 2316 74-75 -0.3425363730393052 2316 76-77 -0.4898114712905439 2316 78-79 -0.5844490879034367 2316 80-81 -0.7066249517093439 2316 82-83 -0.6811753407630583 2316 84-85 -0.8528603846380491 2316 86-87 -0.7362939598495686 2316 88-89 -0.9966089514905825 2316 90-91 -1.5779720657612621 2316 92-93 -1.1845552470936447 2316 94-95 -0.8824935076416729 2316 96-97 -0.7044803249690865 2316 98-99 -1.16694063720497 2316 100-101 -0.25688288972874673 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 6.0 4 11.0 5 33.0 6 97.0 7 199.0 8 389.0 9 680.0 10 1180.0 11 1901.0 12 2733.0 13 4114.0 14 4759.0 15 6141.0 16 8247.0 17 11164.0 18 10335.0 19 6399.0 20 5743.0 21 6017.0 22 6617.0 23 7197.0 24 8100.0 25 9189.0 26 10793.0 27 12763.0 28 15264.0 29 18560.0 30 22291.0 31 26085.0 32 32048.0 33 40632.0 34 54069.0 35 82108.0 36 114031.0 37 155753.0 38 109762.0 39 10293.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.01797003335159 16.733784658270697 13.864179401662602 18.38406590671512 2 12.501241150596249 24.833809935162293 39.60474318111862 23.060205733122835 3 25.704725308550035 29.591512515762613 30.12024266976458 14.583519505922771 4 11.38048216218363 15.707257255766386 40.01692929413283 32.89533128791715 5 11.328974412439308 42.87666438294957 32.80385848897362 12.990502715637506 6 24.3084308877702 37.71521551338953 18.886588623117177 19.089764975723096 7 24.01216824044562 39.08432873611152 19.129854139981926 17.773648883460925 8 20.951366755036588 31.07245340720662 21.29665485091299 26.679524986843806 9 25.561496529742932 23.160118356120858 21.752281234795905 29.526103879340305 10-11 22.392590827400632 25.1063045485687 26.984289515752685 25.516815108277978 12-13 34.20617249014526 22.90804067002274 24.51123489519724 18.374551944634753 14-15 28.49787043110815 24.17263936838408 30.637803245583385 16.691686954924386 16-17 18.111636191002205 28.580329466083736 33.61596629007785 19.692068052836206 18-19 17.106778668096474 33.23776473742218 33.09757677757588 16.557879816905462 20-21 19.46266867236603 29.900434899168925 28.298047421881982 22.33884900658306 22-23 18.046081439337524 28.39268515484595 26.478334475191883 27.082898930624644 24-25 20.994088392372873 28.516587377731 29.914062626054516 20.575261603841614 26-27 24.912513257212577 23.48895702894463 32.820421165120486 18.77810854872231 28-29 19.40774775848202 25.236811533764257 36.119219961673274 19.236220746080445 30-31 18.27135027255667 32.76929244486809 32.04328140359239 16.916075878982852 32-33 17.7715389274473 31.202153644514613 29.720654235302295 21.305653192735793 34-35 19.923107714362846 26.304919182422136 30.217302936366824 23.554670166848194 36-37 23.373758415340095 26.430157798101355 32.53681148769185 17.6592722988667 38-39 26.430668726342226 22.31810319520722 34.17071267960357 17.080515398846988 40-41 19.593526818689103 26.38661815256617 36.805728834978815 17.214126193765914 42-43 17.174113124595436 31.60280841033623 32.53702128029303 18.686057184775304 44-45 18.406457526447646 32.41876118210912 27.984207433205228 21.190573858238007 46-47 18.278047142769374 29.49305667173265 25.334072233947687 26.894823951550293 48-49 21.272266241696702 27.924461092411107 27.957475698271328 22.845796967620863 50-51 25.00868805417374 29.48148451540516 28.225998629769744 17.283828800651353 52-53 19.26178844836317 29.67870334514909 32.34817004756089 18.711338158926853 54-55 15.719544646669249 28.614851111574474 33.91828760934536 21.74731663241091 56-57 20.475075213726132 32.871004736230674 26.154518284630583 20.49940176541261 58-59 18.8869809563231 29.342292824691064 28.198753755272588 23.57197246371325 60-61 19.259485889930726 25.944555150999122 30.968550305423904 23.827408653646252 62-63 26.971501552080014 24.83961012445187 28.27857509001568 19.910313233452445 64-65 23.79931091318896 26.768639599654463 31.68235481020325 17.74969467695332 66-67 18.524544994191416 27.82802369108258 33.22609792181744 20.42133339290856 68-69 17.70518518702394 33.02020978057563 30.345948052560573 18.928656979839868 70-71 18.63258715359487 31.66150348018627 26.201930237407282 23.503979128811572 72-73 18.46385273003485 28.798355227229848 25.263682444173046 27.47410959856225 74-75 20.23236821462969 29.72301242143517 27.76590410374033 22.27871526019481 76-77 23.12543148755139 25.217531922304254 30.320995640032095 21.336040950112263 78-79 19.38745494623336 25.403622173900093 33.641635141441526 21.56728773842503 80-81 18.10019393375223 32.28410518966721 30.122224808005587 19.493476068574974 82-83 17.86394258933802 30.83731742674729 27.628508732735597 23.670231251179093 84-85 19.296788895177386 26.716697447201454 27.683739934268665 26.302773723352495 86-87 21.958246471051567 26.84497971211831 29.558437298891562 21.638336517938562 88-89 24.395693214714704 22.788712591014185 31.377001876170276 21.43859231810084 90-91 19.47443733747796 25.293162367616144 33.742565083540065 21.489835211365833 92-93 17.27762304767011 29.958024286834867 31.250868805417376 21.513483860077645 94-95 17.604852402371094 31.246028318092005 27.615538709004795 23.533580570532106 96-97 17.33254396155412 28.207319313296196 25.445511006523486 29.014625718626196 98-99 20.256136248547854 26.782912831511325 26.87289624973936 26.088054670201466 100-101 23.225938174296775 27.102254497794153 27.978138278429764 21.693669049479304 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2913.0 1 1906.0 2 1513.5 3 1999.0 4 1501.0 5 1373.5 6 2516.5 7 5041.5 8 6918.0 9 6565.0 10 4848.0 11 3330.0 12 2850.0 13 2690.0 14 2476.5 15 2167.0 16 1912.0 17 1813.5 18 1819.5 19 1900.5 20 2035.5 21 2172.5 22 2357.0 23 2648.5 24 2922.0 25 3427.0 26 4214.5 27 5152.0 28 6094.5 29 6985.0 30 8082.0 31 9170.5 32 10424.0 33 11707.5 34 12884.0 35 13819.0 36 14521.5 37 15067.0 38 14987.0 39 14820.0 40 14716.0 41 14565.0 42 14387.0 43 14284.5 44 16117.0 45 17743.0 46 23651.5 47 42648.5 48 140978.0 49 140394.0 50 45072.0 51 29156.5 52 18880.0 53 12078.0 54 7488.5 55 5360.5 56 4588.5 57 4103.5 58 3673.0 59 3391.0 60 3114.5 61 2890.5 62 2267.5 63 1213.0 64 543.5 65 407.0 66 316.5 67 248.0 68 206.0 69 166.5 70 141.0 71 136.0 72 115.0 73 141.0 74 138.5 75 93.0 76 70.5 77 39.0 78 32.0 79 24.5 80 11.0 81 10.0 82 9.5 83 4.0 84 1.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2661026878357312 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.003040818960809429 16-17 8.067478875616852E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.2411505962487464E-4 26-27 0.0013032081260611838 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.003909624378183551 36-37 0.02246482579210231 38-39 0.009370687001678035 40-41 0.020478984838104317 42-43 0.006516040630305918 44-45 0.0010549780068114345 46-47 3.102876490621866E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 4.344027086870612E-4 60-61 0.0025443587223099302 62-63 0.001365265655873621 64-65 0.0 66-67 0.0 68-69 6.205752981243732E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.22328299226514947 78-79 0.0 80-81 0.0020478984838104316 82-83 0.0 84-85 0.0 86-87 0.09805089710365096 88-89 0.3398890907827192 90-91 0.339578803133657 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 4.344027086870612E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 805704.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.561248867608466 #Duplication Level Percentage of deduplicated Percentage of total 1 76.3783556084597 23.342179338488357 2 12.842987606770533 7.8499548090825115 3 4.499142594501325 4.124982495642379 4 2.1060410609060742 2.574529799510108 5 1.1720947362067624 1.7910339464814378 6 0.6736915575016894 1.2353313209289534 7 0.43484235508634966 0.9302527802379633 8 0.3147001716021798 0.7694104210410645 9 0.22985042952373755 0.6322064561101478 >10 1.1424617591090234 6.310734056065587 >50 0.09396683755486596 1.9705633194971155 >100 0.07403447805654222 4.871397619132624 >500 0.010983136854541976 2.22889595287556 >1k 0.01911879378383233 11.354825681733226 >5k 0.004474611311109694 9.313649785126398 >10k+ 0.0032542627717161415 20.70005221804655 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 41671 5.171998649628151 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 35554 4.412786829902792 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 23163 2.874877126090971 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 16108 1.9992453804374806 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 14623 1.8149345168945417 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 13556 1.6825037482748006 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 11784 1.4625718626195228 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 10050 1.2473563492299902 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9472 1.1756178447668126 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 8807 1.093081330116271 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7063 0.8766246661304896 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 7000 0.8688054173741225 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 6746 0.8372801922294044 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 6353 0.7885029737968285 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 6291 0.7808078401000864 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6260 0.7769602732517152 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6148 0.7630593865737293 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 5482 0.6803987568635628 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 5296 0.6573133557733362 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4191 0.5201662148878496 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 4000 0.4964602384994986 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 3681 0.45686753447916356 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 3579 0.4442077983974263 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3511 0.4357679743429349 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 3440 0.4269558051095688 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 3166 0.3929482787723531 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2971 0.36874584214550254 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 2968 0.3683734969666279 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2721 0.3377170772392839 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 2620 0.3251814562171716 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 2415 0.2997378689940723 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2324 0.28844339856820866 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 2228 0.2765283528442207 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2177 0.2701984848033521 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2073 0.2572905186023651 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 1984 0.2462442782957513 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 1979 0.24562370299762692 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 1913 0.23743210906238518 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1842 0.22861993982901907 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 1694 0.21025091100453763 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1581 0.19622590926692682 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1570 0.19486064361105318 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1563 0.19399183819367907 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 1562 0.1938677231340542 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1524 0.18915135086830898 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1487 0.1845590936621886 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1462 0.18145621717156674 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1428 0.17723630514432098 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1405 0.17438165877294887 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 1382 0.17152701240157675 No Hit TCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTA 1345 0.1669347551954564 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 1335 0.16569360459920765 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1308 0.16234249798933603 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1305 0.16197015281046143 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1295 0.16072900221421266 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1250 0.1551438245310933 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1227 0.15228917815972118 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 1179 0.1463316552977272 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 1135 0.14087059267423274 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1132 0.1404982474953581 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1124 0.1395053270183591 No Hit CATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1122 0.13925709689910934 No Hit GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1083 0.13441660957373924 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 1037 0.128707316830995 No Hit TTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCG 1016 0.12610090057887266 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1003 0.12448740480374926 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 926 0.11493054521263392 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 909 0.11282058919901104 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 901 0.11182766872201205 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 881 0.10934536752951457 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 872 0.10822833199289068 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 813 0.10090554347502309 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 6.205752981243732E-5 0.0 14-15 0.0 0.0 0.0 3.102876490621866E-4 0.0 16-17 0.0 0.0 0.0 4.964602384994986E-4 0.0 18-19 0.0 0.0 0.0 6.826328279368105E-4 0.0 20-21 0.0 0.0 0.0 9.929204769989972E-4 0.0 22-23 0.0 0.0 0.0 0.0022340710732477436 0.0 24-25 0.0 0.0 0.0 0.004344027086870613 0.0 26-27 0.0 0.0 0.0 0.00788130628617954 0.0 28-29 0.0 0.0 0.0 0.017996683645606824 0.0 30-31 0.0 0.0 0.0 0.041826775093582756 0.0 32-33 0.0 0.0 0.0 0.07887512039160784 0.0 34-35 0.0 0.0 0.0 0.15024127967591075 0.0 36-37 0.0 0.0 0.0 0.2589040143774885 0.0 38-39 0.0 0.0 0.0 0.3835775917706751 0.0 40-41 0.0 0.0 0.0 0.5142086920258556 0.0 42-43 0.0 0.0 0.0 0.6383237516507303 0.0 44-45 0.0 0.0 0.0 0.7681481040183492 0.0 46-47 0.0 0.0 0.0 0.9519625073227885 0.0 48-49 0.0 0.0 0.0 1.1794654116151837 0.0 50-51 0.0 0.0 0.0 1.3895301500302841 0.0 52-53 0.0 0.0 0.0 1.58166026232959 0.0 54-55 0.0 0.0 0.0 1.7745971225164576 0.0 56-57 0.0 0.0 0.0 1.9838551130439963 0.0 58-59 0.0 0.0 0.0 2.3263506200788377 0.0 60-61 0.0 0.0 0.0 2.8696642935867267 0.0 62-63 0.0 0.0 0.0 3.286318548747431 0.0 64-65 0.0 0.0 0.0 3.577988938865886 0.0 66-67 0.0 0.0 0.0 3.928055464537845 0.0 68-69 0.0 0.0 0.0 4.309150755116022 0.0 70-71 0.0 0.0 0.0 4.773651365762117 0.0 72-73 0.0 0.0 0.0 5.336947563869609 0.0 74-75 0.0 0.0 0.0 5.863691876917578 0.0 76-77 0.0 0.0 0.0 6.310444034037314 0.0 78-79 0.0 0.0 0.0 6.816399074598115 0.0 80-81 0.0 0.0 0.0 7.356733986675007 0.0 82-83 0.0 0.0 0.0 7.929897332022678 0.0 84-85 0.0 0.0 0.0 8.9099719003505 0.0 86-87 0.0 0.0 0.0 10.013913298183947 0.0 88-89 0.0 0.0 0.0 10.733842701538034 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 25 0.005038478 56.949215 2 ACCGTGC 25 0.005038478 56.949215 8 CGTCACT 55 4.884915E-5 43.296494 1 GCTTATG 170 0.0 42.02307 1 CCGATGT 50 0.00162163 37.970856 9 TGCCGAT 55 0.0025904686 34.514675 7 GTGCTAC 70 2.0210491E-4 34.018673 1 GATAGTC 75 3.0937698E-4 31.638453 7 AGTACTC 495 0.0 30.679708 5 GTAAGCG 45 8.9958083E-4 26.365377 94-95 AAGTACT 530 0.0 25.967407 4 GTGACCC 155 1.4192119E-6 24.497326 9 AAAGTAC 545 0.0 24.381927 3 ATACTGC 105 0.0022070592 22.598892 6 CTTATGT 300 7.2759576E-12 22.146915 2 GGTACAA 130 2.9582274E-4 21.903543 2 GCTTGTC 220 0.0 21.57167 94-95 GAGTACT 6085 0.0 21.411255 12-13 CATGGGG 1805 0.0 21.296797 4 GTGTAGA 225 6.947448E-8 21.167175 1 >>END_MODULE