##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939289_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 216073 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.068828590337525 33.0 31.0 34.0 30.0 34.0 2 32.226728929574726 33.0 31.0 34.0 30.0 34.0 3 32.34710491361716 34.0 31.0 34.0 30.0 34.0 4 35.808092635359344 37.0 35.0 37.0 35.0 37.0 5 35.76048372540762 37.0 35.0 37.0 35.0 37.0 6 35.89430886783633 37.0 35.0 37.0 35.0 37.0 7 35.868729549735505 37.0 35.0 37.0 35.0 37.0 8 35.90579109837879 37.0 35.0 37.0 35.0 37.0 9 37.46414869048886 39.0 37.0 39.0 34.0 39.0 10-11 37.36489519745641 39.0 37.0 39.0 33.5 39.0 12-13 37.371045896525715 39.0 37.0 39.0 34.0 39.0 14-15 38.2960226404965 40.0 37.5 41.0 33.0 41.0 16-17 38.42329675618888 40.0 38.0 41.0 33.5 41.0 18-19 38.654244167480435 40.0 38.0 41.0 34.0 41.0 20-21 38.636021159515536 40.0 38.0 41.0 34.0 41.0 22-23 38.581981552530856 40.0 38.0 41.0 34.0 41.0 24-25 38.38675123685051 40.0 37.5 41.0 33.5 41.0 26-27 38.082828488520086 40.0 37.5 41.0 33.5 41.0 28-29 37.98775413864759 40.0 37.0 41.0 33.0 41.0 30-31 38.003438652677566 40.0 37.0 41.0 33.0 41.0 32-33 37.85793690095477 40.0 37.0 41.0 33.0 41.0 34-35 37.5815927950276 40.0 36.5 41.0 33.0 41.0 36-37 37.33583326005563 39.5 36.0 41.0 32.0 41.0 38-39 37.14493481369722 39.0 36.0 41.0 32.0 41.0 40-41 37.23342805440754 40.0 36.0 41.0 32.0 41.0 42-43 36.994936896326706 40.0 36.0 41.0 31.0 41.0 44-45 36.79639982783596 39.0 35.5 41.0 31.0 41.0 46-47 36.503262786188 39.0 35.0 41.0 30.5 41.0 48-49 36.11923285186025 39.0 35.0 41.0 29.5 41.0 50-51 35.818605286176435 39.0 35.0 40.0 28.0 41.0 52-53 35.756135657856376 39.0 35.0 40.0 28.0 41.0 54-55 35.649942380584335 39.0 35.0 40.0 28.0 41.0 56-57 35.15492680714388 38.5 34.5 40.0 27.0 41.0 58-59 34.853695278910365 38.0 34.0 40.0 26.0 41.0 60-61 34.0929917203908 38.0 33.0 40.0 24.0 41.0 62-63 33.88280118293355 37.0 33.0 40.0 24.0 41.0 64-65 33.5620160778996 37.0 33.0 39.5 22.5 40.5 66-67 32.889500307766355 36.0 32.5 39.0 21.5 40.0 68-69 32.2641607234592 36.0 32.0 38.0 20.5 39.0 70-71 31.473657051089216 35.0 31.5 37.0 17.5 39.0 72-73 30.81822809883697 34.5 30.5 36.5 14.0 37.5 74-75 30.419205546273712 34.0 30.0 36.0 9.5 37.0 76-77 28.494615245773417 32.0 27.5 34.0 4.5 36.0 78-79 29.49121593165273 34.0 29.5 35.0 4.0 36.0 80-81 29.388290068634213 34.0 29.0 35.0 2.0 36.0 82-83 29.027800789547978 34.0 29.0 35.0 2.0 35.5 84-85 28.616435186256496 34.0 29.0 35.0 2.0 35.0 86-87 28.32136361322331 34.0 29.0 35.0 2.0 35.0 88-89 27.666240113295043 33.0 26.5 35.0 2.0 35.0 90-91 27.137201316221834 33.0 25.5 35.0 2.0 35.0 92-93 26.893991845348562 33.0 25.0 34.0 2.0 35.0 94-95 26.33869571857659 32.0 25.0 34.0 2.0 35.0 96-97 25.540893586889617 32.0 23.5 34.0 2.0 35.0 98-99 24.692550665747227 32.0 10.5 34.0 2.0 35.0 100-101 20.680522323474012 27.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19397202694789684 1101 2 0.15181153319478113 1101 3 0.36722900655230717 1101 4 -0.0034311711570111925 1101 5 -0.03001839639716053 1101 6 0.0163157195436483 1101 7 -0.14347995982276274 1101 8 -0.10402774491588218 1101 9 -0.01267157132315333 1101 10-11 0.1677420800930136 1101 12-13 0.09464623102280001 1101 14-15 -0.04782081402493077 1101 16-17 0.03552295376717041 1101 18-19 0.249475154245566 1101 20-21 0.2870618533078755 1101 22-23 -0.37301988689639387 1101 24-25 -0.16970835777362936 1101 26-27 -0.060706434246228014 1101 28-29 0.07654405157315125 1101 30-31 -0.047900387086798446 1101 32-33 0.024753003236149596 1101 34-35 0.08180206479085683 1101 36-37 -0.34405227197295574 1101 38-39 -0.14694225614284306 1101 40-41 0.11495467579762675 1101 42-43 -0.3243060272913283 1101 44-45 -0.46177899403534184 1101 46-47 -0.11321717901390116 1101 48-49 -0.15433176792168268 1101 50-51 -0.11575298478372531 1101 52-53 -0.33055559365742937 1101 54-55 -0.3362156685746456 1101 56-57 -0.34073514401704585 1101 58-59 -0.7679144014396968 1101 60-61 -0.8416068735323066 1101 62-63 -0.40403474912316284 1101 64-65 -0.31865930208937954 1101 66-67 -0.9156714243771518 1101 68-69 -0.6172426998713334 1101 70-71 -0.9529099557241061 1101 72-73 -0.5493916406442807 1101 74-75 -1.0284556776443914 1101 76-77 -0.9142258612405456 1101 78-79 -1.1020672118536368 1101 80-81 -0.41401917722837567 1101 82-83 -0.6830903047789327 1101 84-85 -0.6052551932104784 1101 86-87 -0.37950511463288095 1101 88-89 -0.5596499665760497 1101 90-91 -0.29450414909127076 1101 92-93 -0.24020205661587113 1101 94-95 -0.39093074967356145 1101 96-97 -0.08759809853822631 1101 98-99 0.06519706973024952 1101 100-101 0.6526455278073513 1102 1 0.21657089700439514 1102 2 0.09266605296879504 1102 3 0.21521629468789882 1102 4 0.24418535426671895 1102 5 0.08447666010001598 1102 6 0.29332843140804954 1102 7 0.5348392492167875 1102 8 0.2434298822027614 1102 9 0.09820413489153879 1102 10-11 0.21086531455629398 1102 12-13 0.3333909344126269 1102 14-15 0.24283066902592054 1102 16-17 0.22845197919090765 1102 18-19 0.3439755073529156 1102 20-21 0.24371792675420068 1102 22-23 0.48887100293412544 1102 24-25 0.341679354090779 1102 26-27 0.4576992860927618 1102 28-29 0.45489857134715805 1102 30-31 0.31474085585105627 1102 32-33 0.1999825230101564 1102 34-35 0.05832042637277368 1102 36-37 0.16587887209483654 1102 38-39 0.035481825778056475 1102 40-41 0.23095043850948827 1102 42-43 0.38954460830188964 1102 44-45 0.529878844947703 1102 46-47 0.5846994876527702 1102 48-49 0.4658130060896184 1102 50-51 0.5527233570241847 1102 52-53 0.9691884035177125 1102 54-55 0.6547800941372159 1102 56-57 0.5146232599377569 1102 58-59 0.5211922369783792 1102 60-61 0.8832342281626069 1102 62-63 1.0335976661310724 1102 64-65 1.0515737487580736 1102 66-67 0.4184564004816025 1102 68-69 0.6203544046484382 1102 70-71 0.14441525614030226 1102 72-73 0.1513057463613663 1102 74-75 0.31750124325956364 1102 76-77 0.37836771220578314 1102 78-79 0.479368169502294 1102 80-81 0.7044483368959149 1102 82-83 0.9381579296843405 1102 84-85 0.6603256683714385 1102 86-87 0.8279895887569495 1102 88-89 1.0981448780567185 1102 90-91 0.9990604695527985 1102 92-93 1.1314875620281981 1102 94-95 0.8797260299874523 1102 96-97 0.7025237234956769 1102 98-99 0.47736161775284813 1102 100-101 0.19387081029323028 1103 1 0.25302923033772373 1103 2 0.30099938630212364 1103 3 0.3297996280212345 1103 4 -0.08914797906661676 1103 5 -0.05094000656665543 1103 6 0.27770343140804954 1103 7 0.1858809158834518 1103 8 0.1809298822027614 1103 9 0.24924580155820308 1103 10-11 0.19784448122295828 1103 12-13 0.2500576010792983 1103 14-15 0.5423098356925848 1103 16-17 0.48366031252423625 1103 18-19 -0.1325869926470844 1103 20-21 0.19163459342087208 1103 22-23 0.08001683626745404 1103 24-25 0.06563768742410758 1103 26-27 -0.11000904724058103 1103 28-29 0.027815238013829457 1103 30-31 0.25744918918438486 1103 32-33 0.2025866896768278 1103 34-35 0.19894542637277368 1103 36-37 0.34556637209484364 1103 38-39 -0.11295567422194353 1103 40-41 0.10334627184281686 1103 42-43 -0.21722622503143896 1103 44-45 -0.2722044883856327 1103 46-47 -0.27727967901390116 1103 48-49 -0.052416160577053006 1103 50-51 -0.7832141429758153 1103 52-53 -0.4787282631489447 1103 54-55 -0.7436574058627841 1103 56-57 -0.4593350733955788 1103 58-59 -0.46578692968828506 1103 60-61 -1.0308282718373931 1103 62-63 -0.7710898338689205 1103 64-65 -0.8416554179085907 1103 66-67 -0.11539776618506892 1103 68-69 -0.5150622620182261 1103 70-71 -0.43631391052636914 1103 72-73 -0.273173420305298 1103 74-75 -0.4871862567404328 1103 76-77 -0.14246562112755257 1103 78-79 -0.2602151638310417 1103 80-81 -0.6549266631040815 1103 82-83 -0.5956962369823238 1103 84-85 -0.7381118316285615 1103 86-87 -0.33346874457638265 1103 88-89 -0.39925095527661725 1103 90-91 0.10062296955279848 1103 92-93 0.03773756202819456 1103 94-95 0.03597602998745586 1103 96-97 -0.6854971098376588 1103 98-99 -0.4158675489138197 1103 100-101 -0.9415458563734376 1104 1 0.36282940040575085 1104 2 -0.06273360689515073 1104 3 0.16738466203483426 1104 4 0.30891749712386485 1104 5 0.29674281656259893 1104 6 0.355615846374036 1104 7 0.29206714037324844 1104 8 0.3420693379850732 1104 9 0.32014291040174214 1104 10-11 0.28298478734541277 1104 12-13 0.32642877454868824 1104 14-15 0.13425286290346605 1104 16-17 0.1914090370140329 1104 18-19 0.44054225905359345 1104 20-21 0.3858310219923027 1104 22-23 0.2547617342266406 1104 24-25 0.2852380275601689 1104 26-27 0.244954813303643 1104 28-29 -0.039361632734475904 1104 30-31 -0.02156866795847634 1104 32-33 0.083167046819689 1104 34-35 -0.10855882532790417 1104 36-37 -0.12339621293917702 1104 38-39 -0.13793441571854004 1104 40-41 0.13082288748908155 1104 42-43 -0.02324238149402902 1104 44-45 -0.03947702239923956 1104 46-47 -0.15403350554451833 1104 48-49 0.09474582921886565 1104 50-51 0.12781902028948622 1104 52-53 0.2852492028374414 1104 54-55 0.19750968597394802 1104 56-57 0.36363473952959424 1104 58-59 0.19115397167225723 1104 60-61 0.35931841183607816 1104 62-63 0.4034424790562454 1104 64-65 0.1470244290301892 1104 66-67 0.08905589027752114 1104 68-69 -0.1384147280046193 1104 70-71 0.21972350443962085 1104 72-73 0.2290587225518479 1104 74-75 -0.04867647782887374 1104 76-77 -0.0561029510595219 1104 78-79 0.28469342460433467 1104 80-81 0.4236235069639456 1104 82-83 0.2671864160788999 1104 84-85 0.4781934404802861 1104 86-87 0.4186039595052513 1104 88-89 0.6199879903016132 1104 90-91 0.7940753505051781 1104 92-93 0.8211665586268388 1104 94-95 1.3850406558377948 1104 96-97 0.6885462575092802 1104 98-99 0.9885223320385634 1104 100-101 1.2396828851231625 1105 1 0.5865868459677088 1105 2 0.5223410928412022 1105 3 0.5449112707644517 1105 4 0.467495760965285 1105 5 0.350350862651851 1105 6 0.43512468340167487 1105 7 0.4452260115772333 1105 8 0.258581397354277 1105 9 0.6310988717336414 1105 10-11 0.44268598839999385 1105 12-13 0.5048049096917353 1105 14-15 0.7562502264421909 1105 16-17 0.7440894393184934 1105 18-19 0.5673731349446172 1105 20-21 0.39206820586106517 1105 22-23 0.4379713817220008 1105 24-25 0.5808390430859944 1105 26-27 0.5661148562682001 1105 28-29 0.5890193847442902 1105 30-31 0.683062227461896 1105 32-33 0.7948040939830463 1105 34-35 0.708988289211689 1105 36-37 0.2958255427487515 1105 38-39 0.18043049005238032 1105 40-41 0.2547243619544659 1105 42-43 0.48714478772772907 1105 44-45 0.10512555547401803 1105 46-47 0.004007701368877292 1105 48-49 -0.12317722517035179 1105 50-51 -0.1940245496743742 1105 52-53 -0.18856927192087625 1105 54-55 -0.27300100634125357 1105 56-57 -0.21814438360291177 1105 58-59 0.0945325479831638 1105 60-61 -0.32871254217232604 1105 62-63 -0.7776189407269811 1105 64-65 -0.8090223437459159 1105 66-67 -0.804704484685864 1105 68-69 -0.7758402723850537 1105 70-71 -0.7199064145136056 1105 72-73 -0.940114209778983 1105 74-75 -1.0624080470115693 1105 76-77 -0.43521631889469603 1105 78-79 -0.7080196893493813 1105 80-81 -0.5960401001056788 1105 82-83 -0.8874127441593593 1105 84-85 -0.7037467997306344 1105 86-87 -0.9454229909878684 1105 88-89 -0.577356019072468 1105 90-91 -0.2659041436848426 1105 92-93 -0.20761481038009677 1105 94-95 0.11621925167804292 1105 96-97 -0.16478837778024413 1105 98-99 -0.19227055928064374 1105 100-101 -0.48298824074345603 1106 1 0.29299366123203185 1106 2 0.16222906109887703 1106 3 0.3190272702976529 1106 4 0.42459693963257195 1106 5 0.24527442432765412 1106 6 0.18321664279016403 1106 7 0.026810793932234844 1106 8 -0.06470019909804847 1106 9 0.25369193976958826 1106 10-11 0.33191460317417665 1106 12-13 0.36602558888417747 1106 14-15 0.548127925123481 1106 16-17 0.31395756862180235 1106 18-19 0.3111121943447799 1106 20-21 0.21762544707941345 1106 22-23 0.33885829968209435 1106 24-25 0.21751776872492457 1106 26-27 0.4740737779626727 1106 28-29 0.7495632054935015 1106 30-31 0.41423272576975023 1106 32-33 0.20834126284756138 1106 34-35 0.37148099547846414 1106 36-37 0.6651801932330486 1106 38-39 0.8515006265910685 1106 40-41 0.5655540970460748 1106 42-43 0.7910308887896917 1106 44-45 0.9363193937281906 1106 46-47 0.4648316014739038 1106 48-49 0.9591310955205046 1106 50-51 0.8000937838534554 1106 52-53 1.2086065945746256 1106 54-55 1.0217008258445333 1106 56-57 1.1753321014011746 1106 58-59 0.8993172369783764 1106 60-61 1.129042154991879 1106 62-63 1.0334325238546498 1106 64-65 0.9878034235548228 1106 66-67 1.0912460346279431 1106 68-69 1.2540815387947788 1106 70-71 0.7326901545142839 1106 72-73 1.2983967016459204 1106 74-75 1.392475836755498 1106 76-77 1.8765003341570043 1106 78-79 1.784208108526684 1106 80-81 1.8812267921804704 1106 82-83 2.342870836188407 1106 84-85 1.8169440626803812 1106 86-87 1.404069365179712 1106 88-89 1.5227256707396428 1106 90-91 1.8932042703658034 1106 92-93 2.0751486392639684 1106 94-95 2.415752452751679 1106 96-97 2.3145791096745363 1106 98-99 1.576675642143094 1106 100-101 0.7331746720818444 1107 1 -0.07884774438581132 1107 2 0.04087892816626493 1107 3 -0.24109294701825945 1107 4 -0.06616713388493878 1107 5 -0.2526333320010892 1107 6 -0.29321382767567883 1107 7 -0.20696564809759366 1107 8 -0.1839002915729111 1107 9 -0.1523882821700724 1107 10-11 -0.029569618303106893 1107 12-13 -0.01782598896809162 1107 14-15 0.26967456713018834 1107 16-17 0.05256582200291149 1107 18-19 0.08877951604168999 1107 20-21 0.1118804465014378 1107 22-23 0.09630830546176838 1107 24-25 0.2869548280244274 1107 26-27 -0.2623959943179841 1107 28-29 -0.267405931022509 1107 30-31 -0.1413215454127723 1107 32-33 -0.050634578095689164 1107 34-35 -0.11876290696055491 1107 36-37 -0.030519725851448243 1107 38-39 -0.3659424435742338 1107 40-41 -0.17470714442890056 1107 42-43 -0.21024064051327684 1107 44-45 -0.07945912898594543 1107 46-47 0.03844158875861581 1107 48-49 -0.49372984541118115 1107 50-51 -0.1461436453454823 1107 52-53 0.0213334469616413 1107 54-55 0.231942416412096 1107 56-57 -0.9554967045172233 1107 58-59 -0.1755642796092971 1107 60-61 0.43351562626686757 1107 62-63 0.30824468429853624 1107 64-65 -0.14397324966214597 1107 66-67 -0.08591267534778524 1107 68-69 -0.18374067828995067 1107 70-71 0.06615449389701311 1107 72-73 0.16677030007384985 1107 74-75 -0.11395116590315268 1107 76-77 -0.2181592427705219 1107 78-79 -0.33457584708538235 1107 80-81 -0.1300204624721708 1107 82-83 0.08247319429729671 1107 84-85 0.6049223310886624 1107 86-87 0.4132359355184043 1107 88-89 0.41431045862544025 1107 90-91 0.7373725746080879 1107 92-93 0.14080578872645688 1107 94-95 0.18753606158302816 1107 96-97 0.11300703708177195 1107 98-99 0.3713930158571159 1107 100-101 0.1880700598982834 1108 1 0.3557146278912455 1108 2 0.20973409578224533 1108 3 0.37375987266955235 1108 4 0.14771173041350494 1108 5 -0.07182112277460106 1108 6 -0.07431300590081946 1108 7 0.05643710854400297 1108 8 0.20520358250857385 1108 9 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-0.6980312478701052 2316 60-61 -0.47992865062526846 2316 62-63 -0.9027186217477166 2316 64-65 -0.5743732209388952 2316 66-67 -0.5119413267911241 2316 68-69 -0.25251491353338196 2316 70-71 -0.7507078499203068 2316 72-73 -0.4005408445477201 2316 74-75 -0.6517222415889172 2316 76-77 -0.916376605976037 2316 78-79 -1.0028761486795226 2316 80-81 -1.0389228752252961 2316 82-83 -0.8049765400126283 2316 84-85 -0.544456528598257 2316 86-87 -1.317133517303656 2316 88-89 -1.5739668643675238 2316 90-91 -1.8993770304472015 2316 92-93 -2.0022719076687743 2316 94-95 -1.5890239700125441 2316 96-97 -1.274038776504323 2316 98-99 -1.0780361095198785 2316 100-101 -0.5859587351613165 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 0.0 5 6.0 6 41.0 7 46.0 8 99.0 9 177.0 10 316.0 11 459.0 12 587.0 13 759.0 14 987.0 15 1235.0 16 1557.0 17 2064.0 18 1964.0 19 1522.0 20 1650.0 21 1802.0 22 1955.0 23 2181.0 24 2276.0 25 2603.0 26 2798.0 27 3154.0 28 3998.0 29 4609.0 30 5842.0 31 7071.0 32 8674.0 33 10764.0 34 14853.0 35 22291.0 36 33719.0 37 48073.0 38 24912.0 39 1028.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.42520733841063 19.694248360553026 15.517313395430431 15.36323090560591 2 13.642611524808746 28.635692566863973 30.76737954302481 26.954316365302468 3 31.703637196688156 23.64062145663734 31.771206953205628 12.884534393468874 4 11.394297297672546 16.91881910280322 43.53713791172428 28.149745687799953 5 9.125619582270806 44.18830672967006 34.31479176019216 12.37128192786697 6 23.74382731761951 38.82437879790626 14.443266858885654 22.988527025588574 7 20.973004493851615 36.081324367227744 22.887172390812363 20.058498748108278 8 21.88843585269793 22.793685467411475 20.864244954251575 34.45363372563902 9 16.79432414045253 26.91266377566841 28.91661614361813 27.376395940260927 10-11 25.524706927751268 23.03781592332221 25.868109388956512 25.569367759970007 12-13 33.88091061817071 22.710611691419103 24.13397324052519 19.274504449884994 14-15 24.23468266144615 27.541010920046926 32.637495632212634 15.586810786294288 16-17 17.35128117905961 29.802772706130636 31.7794423579341 21.066503756875647 18-19 16.18438255678071 36.79875967557186 28.152356269307756 18.864501498339678 20-21 21.87755989873793 26.936498313070118 23.809545847931023 27.376395940260927 22-23 17.777556659092067 27.731599968529153 28.56951123000097 25.921332142377807 24-25 26.960795657023322 27.781814479365767 25.043850920753634 20.213538942857276 26-27 23.496688619124093 22.005525910224787 36.43004910377511 18.06773636687601 28-29 16.929000846935992 35.73769050274676 28.468850805052 18.86445784526526 30-31 19.258537623858604 27.07857992437741 27.50251072554183 26.16037172622216 32-33 20.425735746715233 29.16051519625312 30.01693871978452 20.396810337247135 34-35 25.829308760879073 24.873361919946685 27.856443674112608 21.440885645061634 36-37 21.294084976395446 23.72951957789503 35.99949088216236 18.976904563547162 38-39 20.714399764890466 28.384848393688145 32.1540060026982 18.74674583872319 40-41 19.019155917785522 30.475218564440066 30.44490417412956 20.060721343644854 42-43 17.60154022733163 31.00633122292569 24.587868858528637 26.80425969121404 44-45 21.24671982820462 24.642362927000846 27.077673542492974 27.033243702301558 46-47 25.942912679771833 29.370928739196334 28.14356292448137 16.542595656550464 48-49 22.858299876198963 26.645454650638094 29.455391130291915 21.040854342871025 50-51 14.156128151430655 29.482233972393523 36.792280368857675 19.569357507318145 52-53 19.804186548064777 32.59477121158127 25.88106797239822 21.719974267955738 54-55 15.928413082615597 25.86972921188672 30.347845404099537 27.85401230139814 56-57 23.357476743647894 24.474707270791875 27.156708474105614 25.01110751145462 58-59 28.413014300920995 24.1076965798121 26.8028879529782 20.676401166288702 60-61 20.84665830529497 28.050242279229703 33.585871441596126 17.517227973879198 62-63 17.99141491860371 28.25371113862245 32.02420483865369 21.730669104120146 64-65 16.7161098332508 34.893299949554084 27.89497068120496 20.49561953599015 66-67 20.40616090932652 26.974804694731386 24.64919101040394 27.96984338553815 68-69 16.74657978043949 28.15039709721014 28.621082251883667 26.4819408704667 70-71 24.962859774242965 27.47242829969501 24.49843339982321 23.06627852623882 72-73 21.15257343582956 22.367209230213863 34.10699161857335 22.373225715383228 74-75 16.347522243691355 34.30283816774462 26.81461083664048 22.535028751923544 76-77 19.344437227428784 25.15078407720145 27.142061798274103 28.36271689709567 78-79 19.692927142510037 26.56585173957815 30.065371576669868 23.675849541241945 80-81 23.719992780217797 24.818464130178228 27.200298047419157 24.261245042184818 82-83 19.31754545917352 23.703331744364174 33.45142613838841 23.52769665807389 84-85 18.810540882016728 26.95015110633906 29.981071212044082 24.258236799600137 86-87 18.562047599745206 28.450055011870983 28.548497307313685 24.439400081070126 88-89 17.942376510311803 27.733539546192375 24.56069462151348 29.763389321982338 90-91 20.25594268665581 22.185952848267192 27.531006834727656 30.02709763034935 92-93 22.816362988434463 27.251669574634498 28.27516626325362 21.656801173677415 94-95 20.470396578933972 24.87238109342676 28.692386369421445 25.964835958217826 96-97 13.644231347738959 26.61345934013042 33.65644944069828 26.085859871432337 98-99 18.931333391955498 28.27979432876852 25.277568229255852 27.51130405002013 100-101 16.98681680284907 23.99702874756156 28.416882452717978 30.59927199687139 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1249.0 1 775.5 2 305.0 3 309.0 4 259.5 5 227.5 6 383.0 7 722.5 8 961.0 9 924.5 10 715.0 11 488.5 12 413.5 13 416.0 14 366.5 15 318.0 16 293.0 17 277.5 18 283.0 19 281.5 20 309.0 21 332.0 22 333.5 23 375.0 24 414.0 25 457.0 26 553.5 27 693.0 28 821.0 29 1003.0 30 1349.0 31 1674.5 32 2092.0 33 2762.0 34 3232.5 35 3575.5 36 4006.5 37 4085.0 38 3812.0 39 3544.0 40 3398.5 41 3227.0 42 2877.0 43 2819.5 44 2896.5 45 3157.0 46 6090.0 47 19958.0 48 44544.0 49 41505.5 50 18953.5 51 9784.5 52 5196.5 53 2301.5 54 782.5 55 454.5 56 332.0 57 293.0 58 276.0 59 253.0 60 251.5 61 252.0 62 221.5 63 184.5 64 164.0 65 158.5 66 152.5 67 141.5 68 132.0 69 118.5 70 101.0 71 83.0 72 67.0 73 53.5 74 38.5 75 24.5 76 17.0 77 13.0 78 12.5 79 8.0 80 3.5 81 2.5 82 1.5 83 1.5 84 0.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2795351570996839 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0011570163787238573 16-17 0.0011570163787238573 18-19 2.3140327574477145E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.002082629481702943 36-37 0.006016485169364058 38-39 0.0016198229302134002 40-41 0.0018512262059581716 42-43 4.628065514895429E-4 44-45 9.256131029790858E-4 46-47 2.3140327574477145E-4 48-49 2.3140327574477145E-4 50-51 2.3140327574477145E-4 52-53 0.0 54-55 0.0 56-57 0.0013884196544686287 58-59 0.0013884196544686287 60-61 0.0 62-63 2.3140327574477145E-4 64-65 0.0 66-67 4.628065514895429E-4 68-69 0.0023140327574477145 70-71 0.0 72-73 0.0 74-75 2.3140327574477145E-4 76-77 0.24667589194392636 78-79 2.3140327574477145E-4 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.09742077908854878 88-89 0.35242718895928693 90-91 0.2573204426281858 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0011570163787238573 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 216073.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.423990966016113 #Duplication Level Percentage of deduplicated Percentage of total 1 74.84870145341911 14.5386050084925 2 10.695735048844412 4.155077219273116 3 4.205384798665714 2.4505606901371295 4 2.3111746485584943 1.7956894197794264 5 1.5320467000238265 1.4879230630388804 6 1.1603526328329758 1.3523207434524442 7 0.7672146771503455 1.0431659670574296 8 0.5503931379556826 0.8552665071526753 9 0.5003573981415297 0.874704382315236 >10 3.0021443888491777 10.728781476630584 >50 0.19061234214915415 2.5801465245542015 >100 0.13342863950440792 5.6471655412754025 >500 0.03097450559923755 4.268927630939544 >1k 0.06433166547533953 32.25206296020326 >5k 0.002382654276864427 3.2887033548846913 >10k+ 0.004765308553728854 12.680899510813475 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 16930 7.835314916717961 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 10470 4.845584594095514 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7106 3.2887033548846913 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4858 2.2483142271361993 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4848 2.2436861616213037 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4624 2.1400174940876466 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4390 2.031720761039093 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4187 1.9377710310867162 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4155 1.9229612214390506 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3965 1.8350279766560376 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3830 1.7725490922049492 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3064 1.4180392737639593 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3045 1.409245949285658 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2692 1.2458752366098496 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2689 1.2444868169553809 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2583 1.1954293224974892 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2282 1.0561245504991368 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1894 0.8765556085211943 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1666 0.7710357147815784 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1659 0.7677960689211516 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1616 0.7478953872071012 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1535 0.7104080565364483 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1525 0.7057799910215529 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1442 0.6673670472479208 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1418 0.6562596900121718 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1365 0.6317309427832261 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1142 0.528525081801058 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1122 0.5192689507712671 No Hit CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT 1069 0.4947402035423213 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1023 0.4734511021738024 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 962 0.4452199025329402 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 907 0.4197655422010154 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 826 0.3822782115303624 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 775 0.35867507740439575 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 754 0.34895613982311535 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 733 0.33923720224183496 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 681 0.3151712615643787 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 670 0.31008038949799377 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 628 0.290642514335433 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 588 0.27213025227585125 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 570 0.26379973434903947 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 565 0.26148570159159173 No Hit ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT 565 0.26148570159159173 RNA PCR Primer, Index 31 (95% over 22bp) CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 479 0.22168433816349103 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 433 0.2003952367949721 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 404 0.1869738468017753 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 378 0.1749408764630472 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 367 0.16985000439666223 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 355 0.16429632577878772 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 353 0.16337071267580863 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 350 0.16198229302134 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 348 0.16105667991836092 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 347 0.16059387336687136 No Hit TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA 331 0.1531889685430387 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 328 0.15180054888857006 No Hit ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT 326 0.15087493578559097 RNA PCR Primer, Index 31 (95% over 24bp) GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 323 0.14948651613112235 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 322 0.14902370957963282 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 318 0.14717248337367464 No Hit GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 317 0.14670967682218508 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 286 0.13236267372600927 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 278 0.1286602213140929 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 268 0.12403215579919749 No Hit CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA 257 0.11894128373281251 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 248 0.11477602476940663 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 243 0.11246199201195892 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 219 0.1013546347762099 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.628065514895429E-4 0.0 10-11 0.0 0.0 0.0 4.628065514895429E-4 0.0 12-13 0.0 0.0 0.0 4.628065514895429E-4 0.0 14-15 0.0 0.0 0.0 9.256131029790858E-4 0.0 16-17 0.0 0.0 0.0 0.0011570163787238573 0.0 18-19 0.0 0.0 0.0 0.0013884196544686287 0.0 20-21 0.0 0.0 0.0 0.002082629481702943 0.0 22-23 0.0 0.0 0.0 0.006710694996598372 0.0 24-25 0.0 0.0 0.0 0.012727180165962429 0.0 26-27 0.0 0.0 0.0 0.0226775210229876 0.0 28-29 0.0 0.0 0.0 0.06294169100257782 0.0 30-31 0.0 0.0 0.0 0.15249475871580437 0.0 32-33 0.0 0.0 0.0 0.34803052672013624 0.0 34-35 0.0 0.0 0.0 0.6053509693483221 0.0 36-37 0.0 0.0 0.0 0.9177453916037636 0.0 38-39 0.0 0.0 0.0 1.3254779634660507 0.0 40-41 0.0 0.0 0.0 1.701971093102794 0.0 42-43 0.0 0.0 0.0 2.11109208461955 0.0 44-45 0.0 0.0 0.0 2.594030721098888 0.0 46-47 0.0 0.0 0.0 3.1813322349391178 0.0 48-49 0.0 0.0 0.0 3.6330314291929113 0.0 50-51 0.0 0.0 0.0 4.0657555548356346 0.0 52-53 0.0 0.0 0.0 4.5007937132358045 0.0 54-55 0.0 0.0 0.0 5.0068726772896195 0.0 56-57 0.0 0.0 0.0 5.758470516908638 0.0 58-59 0.0 0.0 0.0 6.337441512822055 0.0 60-61 0.0 0.0 0.0 7.029337307298922 0.0 62-63 0.0 0.0 0.0 7.681894544899178 0.0 64-65 0.0 0.0 0.0 8.339079848014329 0.0 66-67 0.0 0.0 0.0 9.149916926224009 0.0 68-69 0.0 0.0 0.0 10.079463884890755 0.0 70-71 0.0 0.0 0.0 11.03168836458049 0.0 72-73 0.0 0.0 0.0 11.832343698657398 0.0 74-75 0.0 0.0 0.0 12.897030170359091 0.0 76-77 0.0 0.0 0.0 13.735172835106653 0.0 78-79 0.0 0.0 0.0 15.22864957676341 0.0 80-81 0.0 0.0 0.0 16.56963155970436 0.0 82-83 0.0 0.0 0.0 17.583640714017946 0.0 84-85 0.0 0.0 0.0 18.883201510600585 0.0 86-87 0.0 0.0 0.0 20.07261434792871 0.0 88-89 0.0 0.0 0.0 21.4117451046637 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTTATA 45 1.4933263E-5 52.88765 1 GTAAGCG 65 0.0 43.80524 94-95 TGGTAGG 50 0.0016203261 37.964542 6 TTGGTAG 55 0.0025868248 34.513226 5 CCCACAA 30 0.0038905079 31.762081 90-91 GAGTAGC 60 0.0039039433 31.732588 1 CCTATAG 60 0.0039039433 31.732588 1 TATACTC 75 3.087095E-4 31.637121 4 TACTTAT 60 0.003961979 31.63712 7 CTCCGAC 45 2.4249262E-5 31.63712 94-95 CACTGAC 60 0.003961979 31.63712 7 CTTCTTA 30 0.00396633 31.63712 68-69 TATAGTC 60 0.003961979 31.63712 3 TTCCAAC 80 4.4274068E-4 29.749306 1 ATACTCT 80 4.5062698E-4 29.659801 5 GTCTGAT 65 0.005860382 29.203495 7 TTAGATC 65 0.005860382 29.203495 8 CCTCCGA 65 0.005860382 29.203495 9 ATAGTCT 65 0.005860382 29.203495 4 GTATGTG 100 4.966266E-5 28.473408 9 >>END_MODULE