##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939288_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 218544 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.02568361519877 33.0 31.0 34.0 30.0 34.0 2 32.17873746247895 33.0 31.0 34.0 30.0 34.0 3 32.300461234350976 34.0 31.0 34.0 30.0 34.0 4 35.7754411011055 37.0 35.0 37.0 35.0 37.0 5 35.726540193279156 37.0 35.0 37.0 33.0 37.0 6 35.86945420601801 37.0 35.0 37.0 35.0 37.0 7 35.834037996925105 37.0 35.0 37.0 35.0 37.0 8 35.88139230543964 37.0 35.0 37.0 35.0 37.0 9 37.45629713009737 39.0 37.0 39.0 34.0 39.0 10-11 37.33703281719013 39.0 37.0 39.0 33.5 39.0 12-13 37.35161569294971 39.0 37.0 39.0 34.0 39.0 14-15 38.26405666593455 40.0 37.5 41.0 33.0 41.0 16-17 38.31644886155648 40.0 37.5 41.0 33.5 41.0 18-19 38.579402774727285 40.0 38.0 41.0 34.0 41.0 20-21 38.57762052492862 40.0 38.0 41.0 34.0 41.0 22-23 38.53577998023282 40.0 38.0 41.0 34.0 41.0 24-25 38.34745405959441 40.0 37.5 41.0 33.5 41.0 26-27 38.027710666959514 40.0 37.0 41.0 33.5 41.0 28-29 37.96267799619299 40.0 37.0 41.0 33.0 41.0 30-31 37.97937943846548 40.0 37.0 41.0 33.0 41.0 32-33 37.695818690972985 40.0 36.5 41.0 33.0 41.0 34-35 37.46559274104986 39.5 36.0 41.0 32.5 41.0 36-37 37.3062815725895 39.5 35.5 41.0 32.0 41.0 38-39 37.13171489494107 39.0 35.5 41.0 31.5 41.0 40-41 37.244104162090935 40.0 36.0 41.0 32.0 41.0 42-43 37.013493850208654 40.0 36.0 41.0 31.0 41.0 44-45 36.83560518705615 39.5 35.5 41.0 31.0 41.0 46-47 36.563355662932864 39.0 35.0 41.0 30.5 41.0 48-49 36.18117175488689 39.0 35.0 41.0 30.0 41.0 50-51 35.898926531956945 39.0 35.0 40.0 28.5 41.0 52-53 35.88243328574566 39.0 35.0 40.0 28.5 41.0 54-55 35.73723826780877 39.0 35.0 40.5 28.0 41.0 56-57 35.29336426531957 38.5 34.5 40.0 27.0 41.0 58-59 35.02432919686653 38.0 34.0 40.0 26.5 41.0 60-61 34.286917051028624 38.0 34.0 40.0 25.5 41.0 62-63 34.0538106742807 37.0 33.5 40.0 25.5 41.0 64-65 33.58450929789882 37.0 33.0 39.5 23.0 40.5 66-67 32.87529971081338 36.0 32.0 39.0 22.0 40.0 68-69 32.35539296434585 36.0 32.0 38.0 21.0 39.0 70-71 31.68264743026576 35.0 31.5 37.0 19.0 39.0 72-73 31.04688987114723 35.0 31.0 37.0 15.5 38.0 74-75 30.66412942016253 34.0 31.0 36.0 12.5 37.0 76-77 28.689815323230103 32.5 28.0 34.0 7.0 36.0 78-79 29.696404385386927 34.0 29.5 35.0 7.0 36.0 80-81 29.652166154184055 34.0 30.0 35.0 2.0 36.0 82-83 29.335838549674207 34.0 29.5 35.0 2.0 35.5 84-85 28.97896304634307 34.0 29.0 35.0 2.0 35.0 86-87 28.748684475437443 34.0 29.0 35.0 2.0 35.0 88-89 28.188858994069843 34.0 28.5 35.0 2.0 35.0 90-91 27.705059393074166 33.0 27.0 35.0 2.0 35.0 92-93 27.474714473973204 33.0 27.0 35.0 2.0 35.0 94-95 27.041906892891134 33.0 26.0 35.0 2.0 35.0 96-97 26.43024745588989 33.0 25.0 34.0 2.0 35.0 98-99 25.814456127827803 33.0 24.5 34.0 2.0 35.0 100-101 22.678657387070796 28.5 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.39608145320123356 1101 2 0.31282427379105826 1101 3 0.3333984006603501 1101 4 0.167803257190414 1101 5 0.2078360793330063 1101 6 0.27922508177302774 1101 7 0.2823029985136074 1101 8 0.17042701371374847 1101 9 0.4413474212542994 1101 10-11 0.41520501736959403 1101 12-13 0.35715485854434803 1101 14-15 0.18807064173044097 1101 16-17 0.37191839706417795 1101 18-19 0.42048300332304933 1101 20-21 0.3830663350171264 1101 22-23 0.05790305984205446 1101 24-25 0.11678157890191443 1101 26-27 0.6272335282680217 1101 28-29 0.30411494583750454 1101 30-31 0.5284890489120926 1101 32-33 0.770660979927662 1101 34-35 0.24921156273039458 1101 36-37 0.3809611492956222 1101 38-39 0.44705406611008414 1101 40-41 0.1832273355399039 1101 42-43 0.5027360073911282 1101 44-45 -0.3024848545222625 1101 46-47 -0.2912198051885113 1101 48-49 -0.2356609235837297 1101 50-51 0.042378628041596755 1101 52-53 0.1587676389199828 1101 54-55 0.03147501298844446 1101 56-57 -0.16627118176133848 1101 58-59 -0.3026460247153153 1101 60-61 -0.2978823934896653 1101 62-63 -0.07533060264315594 1101 64-65 -0.2719280109065423 1101 66-67 -0.538349734700418 1101 68-69 -0.21319745317622107 1101 70-71 0.2334794278046921 1101 72-73 0.16926647150046392 1101 74-75 -0.010467558956417378 1101 76-77 0.36738534134664036 1101 78-79 0.38919471232973635 1101 80-81 0.33191191077835214 1101 82-83 0.5426082222187958 1101 84-85 0.34783594817414354 1101 86-87 0.6124334889022052 1101 88-89 0.947120507554704 1101 90-91 1.0990030536778193 1101 92-93 0.8445490217541014 1101 94-95 1.2914540508042016 1101 96-97 1.4559596941426918 1101 98-99 1.7177637457085204 1101 100-101 2.1545322577084107 1102 1 0.17783279009962882 1102 2 0.2357019475878488 1102 3 0.22808979994733392 1102 4 0.04904211637044398 1102 5 -0.10990010605023315 1102 6 -0.1396140447866827 1102 7 -0.029723516637908176 1102 8 -0.05756986685666021 1102 9 0.22493699344681062 1102 10-11 -0.01584723307604463 1102 12-13 0.034614194551473076 1102 14-15 0.24401148397642913 1102 16-17 0.10955208690374718 1102 18-19 0.14831277159400003 1102 20-21 0.043076361755090886 1102 22-23 0.20316542615221778 1102 24-25 0.39478681508730773 1102 26-27 0.0361991031343365 1102 28-29 0.5024493932528387 1102 30-31 0.1664541781456066 1102 32-33 -0.015226386382501289 1102 34-35 0.2926329085414423 1102 36-37 0.34284561899259103 1102 38-39 0.5438677916002845 1102 40-41 0.8785119834900001 1102 42-43 0.599201582257443 1102 44-45 0.6068845750677525 1102 46-47 0.4354123249362587 1102 48-49 0.7949624052932762 1102 50-51 0.4378498847260843 1102 52-53 0.5031303840180215 1102 54-55 0.23285925986902356 1102 56-57 0.051183697917799975 1102 58-59 0.39901674266435805 1102 60-61 0.5382500708597107 1102 62-63 0.6895423920092512 1102 64-65 0.268095384815382 1102 66-67 0.578935247474277 1102 68-69 0.6919384648273379 1102 70-71 0.7907989465212744 1102 72-73 0.6101087174897692 1102 74-75 0.6872959214357444 1102 76-77 0.4086760053395082 1102 78-79 0.100744957427775 1102 80-81 0.5162646291384227 1102 82-83 0.7929285207927741 1102 84-85 0.5669340987980291 1102 86-87 0.9963043667988174 1102 88-89 0.7838935690520294 1102 90-91 0.932698463659996 1102 92-93 0.9752447659608734 1102 94-95 0.7914122727293353 1102 96-97 0.35400726544393635 1102 98-99 0.8457299890240293 1102 100-101 0.280855230079176 1103 1 -0.051781241099284614 1103 2 -7.281873488409474E-4 1103 3 -0.0016350622066667597 1103 4 0.11620567515241476 1103 5 0.3222574955678965 1103 6 -0.12044028955684638 1103 7 0.06919311077612633 1103 8 0.23740672010407593 1103 9 0.09468291348228064 1103 10-11 -0.03183459834716018 1103 12-13 0.12794771260306703 1103 14-15 0.12334428162681377 1103 16-17 0.1481489670123608 1103 18-19 0.17167039537832096 1103 20-21 0.16812138683059885 1103 22-23 0.07016828004930886 1103 24-25 0.5338935175893127 1103 26-27 0.4811438261954848 1103 28-29 0.22215790784096612 1103 30-31 0.30797145392072167 1103 32-33 0.055594593917298596 1103 34-35 0.48639312577011395 1103 36-37 0.4733213608672955 1103 38-39 0.4935782457301201 1103 40-41 0.6189597405485401 1103 42-43 0.6659079633496745 1103 44-45 0.37103630264526544 1103 46-47 0.709940600683673 1103 48-49 0.9071094779706712 1103 50-51 1.0066759078343424 1103 52-53 0.7262330966056396 1103 54-55 0.711621278100715 1103 56-57 0.6724189089122419 1103 58-59 0.4059436773572287 1103 60-61 0.6763592947659376 1103 62-63 0.45365255797860726 1103 64-65 0.5063265227722198 1103 66-67 0.6855546262667715 1103 68-69 0.44974512022618995 1103 70-71 0.8430973034523639 1103 72-73 0.6372068860082365 1103 74-75 0.854318170749977 1103 76-77 0.6314046634537185 1103 78-79 0.9844694316734355 1103 80-81 0.5650995187230841 1103 82-83 0.649540972650577 1103 84-85 0.926622109659462 1103 86-87 0.6079942617864873 1103 88-89 0.9927406500417497 1103 90-91 0.8405563783755774 1103 92-93 1.1249219708041842 1103 94-95 1.1209958254156014 1103 96-97 1.2021411492376828 1103 98-99 0.5800127742059296 1103 100-101 1.429534956326716 1104 1 0.07900030136877234 1104 2 0.06421498059734176 1104 3 -0.013923807018535683 1104 4 0.1989987196685945 1104 5 0.06603852261398657 1104 6 0.07574558788122943 1104 7 0.15733026727451005 1104 8 0.04515432610467229 1104 9 0.011537211830216165 1104 10-11 0.3328942625702922 1104 12-13 0.1725317128200885 1104 14-15 0.059757487280180044 1104 16-17 0.14253358031025698 1104 18-19 0.11954495794783071 1104 20-21 -0.014921230427894727 1104 22-23 0.22613768026089787 1104 24-25 0.18483077174659712 1104 26-27 0.48165924816331085 1104 28-29 0.29792493711151025 1104 30-31 0.37435237788242404 1104 32-33 -0.12720387609328299 1104 34-35 -0.09516561877047991 1104 36-37 0.2876338150896842 1104 38-39 0.2220322511013606 1104 40-41 0.4471207692222805 1104 42-43 0.3692690571293511 1104 44-45 0.6216534526330548 1104 46-47 0.5845709888778003 1104 48-49 0.7143145345314537 1104 50-51 0.7538478688792978 1104 52-53 0.8497580403161322 1104 54-55 0.8415889964090368 1104 56-57 0.9391362135266164 1104 58-59 0.7954806114103405 1104 60-61 0.8250042775399962 1104 62-63 0.6676340570427115 1104 64-65 0.47831740794162414 1104 66-67 0.2223123508144198 1104 68-69 0.027912930767932664 1104 70-71 0.42637864246437474 1104 72-73 0.5419758955842369 1104 74-75 0.785848431444979 1104 76-77 0.7969364975420987 1104 78-79 0.4840218283855826 1104 80-81 0.7638218147923119 1104 82-83 0.2513096838243811 1104 84-85 -0.07208933542096929 1104 86-87 0.08026998541180319 1104 88-89 0.7020697876594149 1104 90-91 0.40204079364291445 1104 92-93 0.41202557061971845 1104 94-95 0.6155296398094343 1104 96-97 0.5606035641252411 1104 98-99 0.406695355656165 1104 100-101 0.6724614680050927 1105 1 0.10350898943311648 1105 2 0.13536352499878745 1105 3 -0.12516138194834525 1105 4 -0.1335252452250444 1105 5 0.1993819730528159 1105 6 0.10999198998558768 1105 7 0.3018531192865552 1105 8 0.19895962240940435 1105 9 0.04717471594028666 1105 10-11 0.2621630487705602 1105 12-13 0.2337022619259841 1105 14-15 0.159651257672472 1105 16-17 -0.21654778651070217 1105 18-19 0.12911826902353596 1105 20-21 -0.1109705007316677 1105 22-23 0.27050118786137745 1105 24-25 0.2184195619937057 1105 26-27 0.3497954244032897 1105 28-29 0.37771096033026197 1105 30-31 0.15941447161740285 1105 32-33 0.13482915625616698 1105 34-35 0.30303100717483744 1105 36-37 0.33933222417484643 1105 38-39 0.31752508060284157 1105 40-41 0.5863447872307361 1105 42-43 0.9804307537679406 1105 44-45 0.30787142567096737 1105 46-47 0.23467089529457752 1105 48-49 0.1547753686867992 1105 50-51 0.011657009684107322 1105 52-53 0.11679903988616047 1105 54-55 -0.43942625512749345 1105 56-57 -0.18555711171303102 1105 58-59 -0.2163311092563731 1105 60-61 -0.0832462219920842 1105 62-63 -0.182063694430596 1105 64-65 0.001245298272202433 1105 66-67 0.008525265299581974 1105 68-69 0.15017815068851093 1105 70-71 0.017748751476190705 1105 72-73 0.13394038454394064 1105 74-75 0.22409614877305373 1105 76-77 0.2723898703321481 1105 78-79 0.158858057740364 1105 80-81 0.3999221764788352 1105 82-83 0.3328029685956082 1105 84-85 0.18158443851230643 1105 86-87 0.7943099985640387 1105 88-89 0.7711316790522886 1105 90-91 0.8419831261415887 1105 92-93 1.0386537922855013 1105 94-95 0.8205527826882602 1105 96-97 0.7322655516306149 1105 98-99 0.755014651505622 1105 100-101 0.4522753132639643 1106 1 0.14927912761984174 1106 2 0.2065015993724515 1106 3 0.3324051836060846 1106 4 0.12109783083382553 1106 5 0.2958205754570429 1106 6 0.05543632208311067 1106 7 -0.25255649761042775 1106 8 0.07425453309359398 1106 9 0.24672532823104376 1106 10-11 0.15981499798974852 1106 12-13 0.15971057559860924 1106 14-15 0.1865808161490463 1106 16-17 0.5239532807851077 1106 18-19 0.588021763012982 1106 20-21 0.42427273036596347 1106 22-23 0.2902068389118213 1106 24-25 0.3496183037081195 1106 26-27 0.369130330593606 1106 28-29 0.17710428692277702 1106 30-31 0.4499885644159676 1106 32-33 0.6163368520206873 1106 34-35 0.34153229916450556 1106 36-37 0.6088075640242963 1106 38-39 0.7169184071953651 1106 40-41 0.5191163859275036 1106 42-43 0.7437607166934512 1106 44-45 0.6684938276482768 1106 46-47 0.8118082955866797 1106 48-49 0.9771661112999865 1106 50-51 0.8191954885067148 1106 52-53 0.8563451582998169 1106 54-55 0.6764483657016243 1106 56-57 0.9078930624247121 1106 58-59 0.976096707842828 1106 60-61 0.6540192537971521 1106 62-63 1.0556286996824227 1106 64-65 1.3311969890934563 1106 66-67 1.2030989087104444 1106 68-69 1.1416959576764896 1106 70-71 1.2571712882698058 1106 72-73 1.4352548435934835 1106 74-75 1.123410832516452 1106 76-77 1.0207889266179535 1106 78-79 0.9702388014770271 1106 80-81 1.2836318720186597 1106 82-83 1.280629055552133 1106 84-85 1.1721697660035986 1106 86-87 1.5277556788246827 1106 88-89 2.1192498680198177 1106 90-91 1.681585418018905 1106 92-93 1.5914119093509989 1106 94-95 2.0116212019669923 1106 96-97 2.217817736778347 1106 98-99 2.0946169433829382 1106 100-101 1.7114484398789536 1107 1 0.04992760704738686 1107 2 -0.15472380313202194 1107 3 0.167132375019321 1107 4 -0.0886069992198415 1107 5 0.0507847972817288 1107 6 0.00919303049097664 1107 7 -0.2998284117428085 1107 8 -0.041912729875996035 1107 9 -0.13797950182262042 1107 10-11 0.018770722497791326 1107 12-13 -0.17790123119924317 1107 14-15 0.11415237249967447 1107 16-17 0.06422608937187135 1107 18-19 0.29868813152818063 1107 20-21 0.2568644119402066 1107 22-23 0.11259055984205446 1107 24-25 0.13861651479935233 1107 26-27 0.1578825667295547 1107 28-29 0.13784892019648254 1107 30-31 0.08157398480952338 1107 32-33 -0.003978443149257771 1107 34-35 0.21756092170475227 1107 36-37 0.3194627518597244 1107 38-39 0.7723745789305951 1107 40-41 0.6593891945142687 1107 42-43 0.730500430468048 1107 44-45 0.2592138634264529 1107 46-47 0.4856231435294305 1107 48-49 0.4800842687239637 1107 50-51 0.30079208958006376 1107 52-53 0.112293279945618 1107 54-55 0.29249264119358287 1107 56-57 0.04226247208481482 1107 58-59 -0.10012198625378232 1107 60-61 0.3076865167667435 1107 62-63 0.20832324351069076 1107 64-65 0.6138892967857643 1107 66-67 0.11749962427394678 1107 68-69 -0.08018463266340348 1107 70-71 -0.08142441834915459 1107 72-73 -0.11679122080722948 1107 74-75 -0.3640332640846218 1107 76-77 -0.008616261217465393 1107 78-79 0.2916386225861487 1107 80-81 0.3323125518039909 1107 82-83 0.11812905555213149 1107 84-85 0.33481511484080784 1107 86-87 0.05133573249194612 1107 88-89 0.5186349306316274 1107 90-91 0.3341793357291003 1107 92-93 -0.1444333500407744 1107 94-95 0.018016550804201614 1107 96-97 0.8247497582452574 1107 98-99 0.7568262457085204 1107 100-101 0.3911107833494363 1108 1 -0.32713283251305114 1108 2 -0.2474435833517994 1108 3 -0.180738504101555 1108 4 -0.1432086475714911 1108 5 -0.2445448730479427 1108 6 -0.19547729917935186 1108 7 -0.5443339062482977 1108 8 -0.40248965295291583 1108 9 -0.13900972160284653 1108 10-11 -0.37906581596374167 1108 12-13 -0.20571716526518458 1108 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-1.1818898162369962 2316 96-97 -1.2707198470558119 2316 98-99 -1.3438291850030915 2316 100-101 -1.0882989688833504 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 4.0 5 15.0 6 42.0 7 54.0 8 105.0 9 166.0 10 298.0 11 416.0 12 567.0 13 811.0 14 916.0 15 1261.0 16 1573.0 17 2003.0 18 1949.0 19 1522.0 20 1574.0 21 1761.0 22 1843.0 23 2007.0 24 2303.0 25 2490.0 26 2659.0 27 3128.0 28 3737.0 29 4658.0 30 5631.0 31 6754.0 32 8510.0 33 10654.0 34 14241.0 35 21085.0 36 32000.0 37 50266.0 38 29884.0 39 1656.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.6969780383629 19.774648921186763 15.354794903909236 15.1735781365411 2 13.73590672816458 28.86787100080533 30.721502306171754 26.674719964858333 3 31.740061497913462 23.848744417600116 31.507156453620322 12.904037630866094 4 11.476865070649389 17.04691046196647 43.49101325133611 27.98521121604803 5 9.143696463869976 44.21352221978183 34.17252361080606 12.470257705542133 6 23.78468409107548 38.92076652756424 14.235119701295847 23.05942968006443 7 21.14722893330405 36.270041730726994 22.873654733142985 19.709074602825975 8 21.878431803206677 22.97294823925617 20.762866974156232 34.38575298338092 9 17.09998901822974 26.952924811479612 28.702229299363058 27.244856870927592 10-11 25.698943919759863 23.133785416209093 25.701689362325208 25.465581301705836 12-13 33.964327549600995 22.994682992898454 23.90822900651585 19.132760450984698 14-15 24.321343836440718 27.68345165725948 32.44210974267692 15.553094763622886 16-17 17.34597460002242 29.93751816000018 31.7158826503648 21.000624589612592 18-19 16.31708031334651 36.98408558459624 27.81636649827952 18.882467603777727 20-21 21.917325572882348 27.060225858408376 23.809850647924446 27.21259792078483 22-23 17.97509883593235 27.81407862947507 28.414186616882642 25.79663591770994 24-25 27.008581815519566 27.822378611123188 24.99571023617724 20.173329337180014 26-27 23.466306093593424 22.076406715444698 36.37898609414252 18.07830109681936 28-29 16.994975840105425 35.851819313273296 28.31946884837836 18.83373599824292 30-31 19.48440588622886 27.085392415257342 27.353530639139027 26.076671059374775 32-33 20.602029797203308 29.163692437220877 29.935390584962295 20.298887180613516 34-35 25.820282560201342 25.167305382371445 27.774867013670423 21.237545043756793 36-37 21.364251110444936 24.016503923457677 35.76010270328408 18.85914226281331 38-39 20.632233087902183 28.568780974433366 32.12188882838276 18.67709710928169 40-41 19.15543107156914 30.647545560223705 30.179357626017158 20.01766574219 42-43 17.62979131345995 31.194073219895348 24.35347441010188 26.822661056542817 44-45 21.349394282576615 24.72814212338561 27.106626857476236 26.815836736561536 46-47 26.06500292847207 29.431601874222125 27.996192986309392 16.507202210996414 48-49 22.926962076286696 26.6927941284135 29.44967603777729 20.93056775752251 50-51 14.096017277985212 29.597701149425287 36.71457463943187 19.591706933157624 52-53 19.930311516216413 32.69593308441321 25.725711984771944 21.648043414598433 54-55 16.088751006662275 25.952897357053956 30.208790907094222 27.749560729189543 56-57 23.387738853503183 24.54883227176221 27.18079654440296 24.88263233033165 58-59 28.51779402175778 24.21062264776874 26.724550144708697 20.547033185764782 60-61 21.013869378005758 28.129075359546807 33.25996732848599 17.597087933961443 62-63 17.892294809669583 28.51798975935866 31.928333813792374 21.661381617179384 64-65 16.945329087048833 34.99867303609342 27.59444322424775 20.46155465261 66-67 20.485348488176296 27.124286184933013 24.57605791053518 27.814307416355515 68-69 16.915167918515078 28.221841418298876 28.593620949604997 26.26936971358105 70-71 24.964309246650558 27.613890109085588 24.456402372062378 22.96539827220148 72-73 21.178801522805475 22.41791126729629 34.1169283988579 22.28635881104034 74-75 16.394639065817408 34.291721575517975 26.717960685262465 22.595678673402155 76-77 19.46193605789737 25.26841282144184 27.128842740585483 28.140808380075306 78-79 19.815231715352517 26.62347170363863 29.930586060472947 23.63071052053591 80-81 23.636068289245497 25.104213854608513 27.106584119227055 24.15313373691893 82-83 19.361089757668935 24.0022604143788 33.374514971813454 23.26213485613881 84-85 18.807196720111282 27.045125924298997 30.039717402445277 24.107959953144448 86-87 18.653356417866696 28.611309589241873 28.370158660364226 24.365175332527205 88-89 17.93178661217555 27.789551022282428 24.70812430403288 29.570538061509144 90-91 20.355829235029134 22.378807290159937 27.52200686035459 29.74335661445634 92-93 22.82789735705396 27.330194377333626 28.151539278131636 21.69036898748078 94-95 20.546892159016032 25.006863606413354 28.668597627937626 25.777646606632988 96-97 13.696097810967128 26.618209605388387 33.57058532835493 26.115107255289555 98-99 18.968491470825096 28.334339995607294 25.372465041364666 27.324703492202946 100-101 17.06742868647674 24.066312196282635 28.44624831840104 30.42001079883958 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1250.0 1 777.5 2 305.0 3 296.0 4 243.0 5 230.0 6 385.0 7 746.5 8 979.5 9 954.5 10 732.5 11 500.5 12 445.0 13 399.0 14 369.5 15 322.5 16 298.0 17 309.0 18 291.0 19 289.0 20 308.0 21 328.5 22 360.5 23 381.5 24 427.5 25 502.5 26 622.0 27 725.5 28 794.5 29 971.5 30 1337.5 31 1666.5 32 2101.5 33 2811.0 34 3267.5 35 3594.0 36 4016.5 37 4128.0 38 3995.0 39 3734.0 40 3399.5 41 3268.0 42 2989.5 43 2849.0 44 2962.5 45 3238.5 46 6127.5 47 20040.5 48 44877.0 49 41931.0 50 19111.0 51 9867.0 52 5298.5 53 2319.0 54 789.5 55 485.0 56 377.0 57 324.0 58 279.5 59 254.5 60 262.5 61 232.0 62 206.0 63 207.0 64 183.5 65 160.0 66 156.0 67 147.0 68 118.5 69 100.5 70 87.5 71 79.5 72 69.0 73 49.5 74 35.5 75 26.0 76 23.0 77 16.0 78 9.5 79 6.0 80 3.0 81 3.0 82 1.5 83 1.0 84 0.5 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26218976499011637 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0025166556848964052 16-17 2.2878688044512775E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.2878688044512775E-4 26-27 0.0013727212826707665 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0029742294457866607 36-37 0.0231074749249579 38-39 0.0077787539351343435 40-41 0.01921809795739073 42-43 0.004804524489347682 44-45 6.863606413353832E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 6.863606413353832E-4 60-61 0.0022878688044512775 62-63 0.0013727212826707665 64-65 0.0 66-67 0.0 68-69 2.2878688044512775E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.23015960172779854 78-79 0.0 80-81 0.0013727212826707665 82-83 0.0 84-85 0.0 86-87 0.0988359323522952 88-89 0.3530181565268321 90-91 0.35278936964638696 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 9.15147521780511E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 218544.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.37824877370232 #Duplication Level Percentage of deduplicated Percentage of total 1 74.89256198347107 14.512866974156232 2 10.25029515938607 3.9726553920491985 3 4.413223140495868 2.5656160773116627 4 2.4439197166469895 1.894355370085658 5 1.5631641086186543 1.5145691485467456 6 1.1357733175914995 1.3205578739292774 7 0.8028335301062574 1.089025550918808 8 0.6115702479338843 0.9480928325646095 9 0.46989374262101535 0.8195146057544477 >10 2.9917355371900825 10.754813675964565 >50 0.18890200708382526 2.4992678819825755 >100 0.1322314049586777 5.554945457207702 >500 0.03305785123966942 4.510304561095248 >1k 0.06375442739079103 32.20770188154331 >5k 0.0023612750885478157 3.215828391536716 >10k+ 0.004722550177095631 12.619884325353247 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 17042 7.7979720330917335 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 10538 4.821912292261513 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7028 3.215828391536716 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4988 2.282377919320594 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4742 2.1698147741415914 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4741 2.1693572003807016 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4471 2.0458122849403324 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4470 2.045354711179442 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4136 1.892525075042097 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4084 1.8687312394758036 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3841 1.7575408155794714 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3072 1.4056665934548649 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3049 1.395142396954389 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2625 1.2011311223369208 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2597 1.1883190570319935 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2573 1.1773372867706273 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2211 1.011695585328355 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1915 0.8762537521048392 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1730 0.7916026063401421 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1684 0.7705542133391903 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1678 0.7678087707738488 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1623 0.7426422139248847 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1476 0.6753788710740172 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1474 0.6744637235522366 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1471 0.6730910022695659 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1429 0.6538729043121752 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1190 0.544512775459404 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1055 0.48274031773921955 No Hit CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT 1034 0.47313126876052425 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1029 0.4708433999560729 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 987 0.45162530199868217 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 933 0.42691631891060833 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 818 0.37429533640822904 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 815 0.37292261512555824 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 758 0.34684091075481366 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 754 0.3450106157112527 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 711 0.3253349439929717 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 659 0.30154110842667836 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 623 0.2850684530346292 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 603 0.2759169778168241 No Hit ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT 582 0.2663079288381287 RNA PCR Primer, Index 31 (95% over 22bp) TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 570 0.26081704370744563 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 535 0.24480196207628668 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 509 0.23290504429314005 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 431 0.1972142909437001 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 397 0.18165678307343142 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 392 0.17936891426898016 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 391 0.1789113405080899 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 386 0.17662347170363862 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 380 0.1738780291382971 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 365 0.16701442272494324 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 355 0.1624386851160407 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 343 0.15694779998535763 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 337 0.15420235742001612 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 336 0.15374478365912583 No Hit TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA 324 0.14825389852844278 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 315 0.14413573468043048 No Hit ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT 311 0.14230543963686945 RNA PCR Primer, Index 31 (95% over 24bp) GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 302 0.13818727578885717 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 301 0.1377297020279669 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 288 0.13178124313639358 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 286 0.13086609561461307 No Hit CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA 256 0.11713888278790541 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 256 0.11713888278790541 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 234 0.1070722600483198 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 230 0.10524196500475878 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 223 0.10203894867852699 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.2878688044512775E-4 0.0 16-17 0.0 0.0 0.0 4.575737608902555E-4 0.0 18-19 0.0 0.0 0.0 6.863606413353832E-4 0.0 20-21 0.0 0.0 0.0 0.001830295043561022 0.0 22-23 0.0 0.0 0.0 0.0041181638480122995 0.0 24-25 0.0 0.0 0.0 0.010066622739585621 0.0 26-27 0.0 0.0 0.0 0.02539534372940918 0.0 28-29 0.0 0.0 0.0 0.06543304780730654 0.0 30-31 0.0 0.0 0.0 0.15786294750713814 0.0 32-33 0.0 0.0 0.0 0.33997730434145984 0.0 34-35 0.0 0.0 0.0 0.6030822168533567 0.0 36-37 0.0 0.0 0.0 0.9128596529760598 0.0 38-39 0.0 0.0 0.0 1.3136942675159236 0.0 40-41 0.0 0.0 0.0 1.715444029577568 0.0 42-43 0.0 0.0 0.0 2.113761988432535 0.0 44-45 0.0 0.0 0.0 2.57842814261659 0.0 46-47 0.0 0.0 0.0 3.173502818654367 0.0 48-49 0.0 0.0 0.0 3.587607072260048 0.0 50-51 0.0 0.0 0.0 4.02092942382312 0.0 52-53 0.0 0.0 0.0 4.43274580862435 0.0 54-55 0.0 0.0 0.0 4.934017863679625 0.0 56-57 0.0 0.0 0.0 5.7036569294970345 0.0 58-59 0.0 0.0 0.0 6.263727212826708 0.0 60-61 0.0 0.0 0.0 6.978915001098176 0.0 62-63 0.0 0.0 0.0 7.622263708909877 0.0 64-65 0.0 0.0 0.0 8.26355333479757 0.0 66-67 0.0 0.0 0.0 9.07300131781243 0.0 68-69 0.0 0.0 0.0 10.013544183322352 0.0 70-71 0.0 0.0 0.0 10.934182590233545 0.0 72-73 0.0 0.0 0.0 11.744316933889742 0.0 74-75 0.0 0.0 0.0 12.822131927666739 0.0 76-77 0.0 0.0 0.0 13.65239951680211 0.0 78-79 0.0 0.0 0.0 15.145233911706567 0.0 80-81 0.0 0.0 0.0 16.52710666959514 0.0 82-83 0.0 0.0 0.0 17.562367303609342 0.0 84-85 0.0 0.0 0.0 18.801477048100153 0.0 86-87 0.0 0.0 0.0 20.03395197305806 0.0 88-89 0.0 0.0 0.0 21.376930961270958 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTACAA 20 0.0020812512 71.16848 3 GTATAGT 25 0.004993131 57.06537 1 CTACAAT 25 0.005038628 56.934784 4 TATATGA 50 2.8385637E-5 47.445652 2 ATGGGAG 55 4.98362E-5 43.132412 7 GCTTATG 90 1.177068E-8 42.270645 1 CTTATGT 90 1.2010787E-8 42.173916 2 GTAAGCG 50 3.0540832E-8 37.956524 94-95 TATGTAC 100 3.042078E-8 37.956524 4 GAGGGTG 70 2.0617308E-4 33.889755 7 TCAACGA 35 2.0650977E-4 33.889755 94-95 GGAGGGT 85 1.6389764E-5 33.491047 6 TCATCCT 30 0.0038518053 31.827078 86-87 TGTCACA 30 0.0039660386 31.637676 36-37 ATACCAT 60 0.0039661783 31.630436 6 GTACGAA 30 0.0039704847 31.630436 24-25 TTATGTA 125 2.1619962E-7 30.36522 3 AGGGTGA 80 4.5120163E-4 29.653534 8 GAGTACG 65 0.0058012446 29.26429 1 GGAGGGG 65 0.0058665816 29.197323 8 >>END_MODULE