FastQCFastQC Report
Fri 27 May 2016
SRR939287_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939287_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2318700
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64630.27873377323500237No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT56440.2434122568680726No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50250.21671626342347006No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34120.14715142105490145No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT33370.1439168499590288No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG97050.023.7152211
TACATGG96700.023.2416322
GTATCAA155150.022.633981
GAGTACT67050.021.51898612-13
ACATGGG99250.021.4979363
GTACTTT70250.020.40804714-15
TCAACGC176400.019.7293264
AGTACTT70300.019.7153212-13
ATCAACG177300.019.655923
AGAGTAC127100.019.59117310-11
CAACGCA176450.019.5893765
TATCAAC179550.019.3567892
AACGCAG180200.019.2235416
GGTATCA118800.019.1337471
ACCGTGC3255.0386006E-1018.9553078
TACCTGG20250.017.5610712
ACGCAGA195700.017.5072637
GTACACG3802.1100277E-1017.4833811
CATGGGA53550.017.4430144
CGCAGAG197100.017.2626978
Produced by FastQC (version 0.11.5)