##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939287_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2318700 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.64442101177384 33.0 31.0 34.0 30.0 34.0 2 31.713771941173935 33.0 31.0 34.0 30.0 34.0 3 31.761895027386036 34.0 31.0 34.0 30.0 34.0 4 35.24957993703368 37.0 35.0 37.0 33.0 37.0 5 35.27789752878768 37.0 35.0 37.0 33.0 37.0 6 35.361375339629966 37.0 35.0 37.0 33.0 37.0 7 35.28566222452236 37.0 35.0 37.0 33.0 37.0 8 35.26274722905076 37.0 35.0 37.0 33.0 37.0 9 36.848204597403715 39.0 37.0 39.0 33.0 39.0 10-11 36.730210247121235 39.0 37.0 39.0 32.0 39.0 12-13 36.61983137102686 39.0 37.0 39.0 32.0 39.0 14-15 37.79218117910898 40.0 38.0 41.0 32.0 41.0 16-17 37.708300340708156 40.0 38.0 41.0 32.0 41.0 18-19 37.62183745202053 40.0 38.0 41.0 32.0 41.0 20-21 37.58920386423427 40.0 37.5 41.0 32.0 41.0 22-23 37.59541165308147 40.0 38.0 41.0 32.0 41.0 24-25 37.50176111614267 40.0 37.5 41.0 32.0 41.0 26-27 37.410505455643246 40.0 37.0 41.0 31.5 41.0 28-29 37.25520313106482 40.0 37.0 41.0 31.0 41.0 30-31 37.07308836848234 40.0 37.0 41.0 30.5 41.0 32-33 36.85308340880666 40.0 36.0 41.0 30.0 41.0 34-35 36.557060853064215 39.5 36.0 41.0 30.0 41.0 36-37 36.31626967697417 39.0 35.5 41.0 30.0 41.0 38-39 35.71446198300772 38.5 35.0 40.5 27.5 41.0 40-41 35.69983460559797 39.0 35.0 40.0 27.0 41.0 42-43 35.88799801612973 39.0 35.0 41.0 27.5 41.0 44-45 35.793443524388664 39.0 35.0 41.0 27.0 41.0 46-47 35.525109759779184 39.0 35.0 41.0 26.0 41.0 48-49 35.28261827748307 39.0 35.0 41.0 25.0 41.0 50-51 34.16911135550093 38.0 34.0 40.0 23.5 40.5 52-53 34.29742549704576 38.0 33.5 39.5 24.0 40.5 54-55 34.785611549575194 39.0 34.0 40.0 24.0 41.0 56-57 34.68822745503946 38.5 34.0 40.0 23.5 41.0 58-59 34.47019666192263 38.0 34.0 40.0 23.0 41.0 60-61 34.15195971880796 38.0 34.0 40.0 22.5 41.0 62-63 33.76767348082977 37.5 33.5 40.0 21.5 41.0 64-65 33.36859641178246 37.0 33.0 40.0 20.0 41.0 66-67 32.987689438047184 36.5 32.5 39.0 18.5 41.0 68-69 32.571761763056884 36.0 32.0 39.0 17.5 41.0 70-71 32.14044054858326 35.5 32.0 39.0 13.5 40.0 72-73 31.692107215249926 35.0 31.0 38.0 11.5 40.0 74-75 31.211056410919912 35.0 31.0 37.0 7.5 39.0 76-77 30.733980031914435 35.0 30.5 37.0 7.0 39.0 78-79 30.32588713503256 35.0 30.0 36.0 2.0 39.0 80-81 29.89775067925993 34.0 30.0 36.0 2.0 37.5 82-83 29.447857420106097 34.0 29.0 35.0 2.0 37.0 84-85 29.009723983266483 34.0 29.0 35.0 2.0 36.5 86-87 28.564673092681243 34.0 29.0 35.0 2.0 36.0 88-89 28.11415254237288 34.0 27.5 35.0 2.0 36.0 90-91 27.675688532367275 34.0 27.0 35.0 2.0 35.0 92-93 27.19064238581964 33.5 26.0 35.0 2.0 35.0 94-95 26.676949152542374 33.0 25.0 35.0 2.0 35.0 96-97 26.022487385172727 33.0 24.5 35.0 2.0 35.0 98-99 25.182028938629404 32.5 19.0 35.0 2.0 35.0 100-101 22.45018846767585 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.44736953414605907 1101 2 0.5452857805500351 1101 3 0.549533447756481 1101 4 0.4556802769219175 1101 5 0.45413171239139416 1101 6 0.48203986618787553 1101 7 0.5178710385580558 1101 8 0.42388262610946015 1101 9 0.41323904683343926 1101 10-11 0.6385069034418436 1101 12-13 0.704141249605577 1101 14-15 0.8966887570332887 1101 16-17 0.8468302365879339 1101 18-19 0.8874764045861738 1101 20-21 0.8981036278392409 1101 22-23 0.9512368216428229 1101 24-25 0.9505433921997337 1101 26-27 0.8557123197306709 1101 28-29 0.9212483157890787 1101 30-31 0.888798114612392 1101 32-33 0.8762729040947761 1101 34-35 0.8095591085975897 1101 36-37 0.8175394735205757 1101 38-39 1.2252148171886788 1101 40-41 1.018710693018896 1101 42-43 0.9283047252945948 1101 44-45 0.8253400515891656 1101 46-47 0.7584367777769145 1101 48-49 0.7852910371447308 1101 50-51 0.9220319043003187 1101 52-53 0.9206035231733551 1101 54-55 0.8879557897837884 1101 56-57 1.0278140422781021 1101 58-59 0.9710335981316831 1101 60-61 0.9439884589068797 1101 62-63 1.008228493281699 1101 64-65 0.9143959057798909 1101 66-67 0.914100714775067 1101 68-69 0.9151231448606012 1101 70-71 1.0652502331856581 1101 72-73 0.947362436574231 1101 74-75 0.9379321600895203 1101 76-77 1.0141865158762862 1101 78-79 0.9617148464017582 1101 80-81 0.9435555571897183 1101 82-83 1.0408588583122018 1101 84-85 0.9782321784759311 1101 86-87 1.0270054997768234 1101 88-89 0.9899397009876658 1101 90-91 1.0508757215192475 1101 92-93 1.0024861604579272 1101 94-95 1.1228274446094098 1101 96-97 1.1205121724890965 1101 98-99 0.9983669191687916 1101 100-101 0.8005397117116466 1102 1 0.35203563755655054 1102 2 0.3460053263759484 1102 3 0.4236477831839025 1102 4 0.35497313946231657 1102 5 0.33511874913410367 1102 6 0.4500339492096117 1102 7 0.4361138465852932 1102 8 0.4606225439632894 1102 9 0.606624132813657 1102 10-11 0.5660099776494505 1102 12-13 0.5353543556287903 1102 14-15 0.6719840482674044 1102 16-17 0.6622146589296847 1102 18-19 0.6097057139839066 1102 20-21 0.7206284376008583 1102 22-23 0.703682245744055 1102 24-25 0.6596651447629682 1102 26-27 0.5525037811016134 1102 28-29 0.5672634177468083 1102 30-31 0.675113847584349 1102 32-33 0.6099203503414543 1102 34-35 0.5306200796847023 1102 36-37 0.5549164006514005 1102 38-39 1.0117452567236924 1102 40-41 0.9298650475189376 1102 42-43 0.9056859465566802 1102 44-45 0.754673011277319 1102 46-47 0.7738639323015093 1102 48-49 0.7781994466710103 1102 50-51 0.8814614261738924 1102 52-53 0.8208845238732394 1102 54-55 0.8344792165005401 1102 56-57 0.7995394883518969 1102 58-59 0.904700075187364 1102 60-61 1.0165617170650236 1102 62-63 0.8579311107498953 1102 64-65 0.881101052324091 1102 66-67 0.9974615835280147 1102 68-69 0.8237564399928985 1102 70-71 0.9122163876238076 1102 72-73 0.81689299601031 1102 74-75 0.7821527892076325 1102 76-77 0.7931753873841672 1102 78-79 0.7571664133414124 1102 80-81 0.6891481473037366 1102 82-83 0.7625451274092363 1102 84-85 0.6029404424478884 1102 86-87 0.4297361071733228 1102 88-89 0.4515448695602018 1102 90-91 0.6839136241986168 1102 92-93 0.7355748816712158 1102 94-95 0.8167350064630234 1102 96-97 0.7968336635006992 1102 98-99 0.7813787503369305 1102 100-101 0.4741407729755167 1103 1 0.45154117359761514 1103 2 0.44313194595998695 1103 3 0.44962630362913814 1103 4 0.4650226960144792 1103 5 0.35907314811002067 1103 6 0.42781451149311067 1103 7 0.45563652110610064 1103 8 0.41449345825574113 1103 9 0.4761839621265125 1103 10-11 0.6082879542439983 1103 12-13 0.5442579656792645 1103 14-15 0.658127766399538 1103 16-17 0.6289283026558508 1103 18-19 0.7402015365368371 1103 20-21 0.7749760990520898 1103 22-23 0.7420228241470923 1103 24-25 0.6396102777605961 1103 26-27 0.6417627185617718 1103 28-29 0.6848192061604834 1103 30-31 0.645086510469902 1103 32-33 0.7397949374275612 1103 34-35 0.6088384901939676 1103 36-37 0.4704665876187235 1103 38-39 0.9822053611059332 1103 40-41 0.784822745216168 1103 42-43 0.6632566657348349 1103 44-45 0.5837149546542406 1103 46-47 0.6371248883753182 1103 48-49 0.627777961241236 1103 50-51 0.6259119598121785 1103 52-53 0.7116207487168822 1103 54-55 0.5991483598473479 1103 56-57 0.6156735265017659 1103 58-59 0.6846784157106853 1103 60-61 0.7199869574466646 1103 62-63 0.6504308915848114 1103 64-65 0.5523638746287176 1103 66-67 0.5300245802926256 1103 68-69 0.5096335570013437 1103 70-71 0.40155188369192274 1103 72-73 0.5015238554632973 1103 74-75 0.538819653237617 1103 76-77 0.5730285398959829 1103 78-79 0.641821323580114 1103 80-81 0.5217430893867814 1103 82-83 0.479259344865973 1103 84-85 0.47029909703591954 1103 86-87 0.5117225506083081 1103 88-89 0.49016244508494466 1103 90-91 0.4363059659511066 1103 92-93 0.3842628975410598 1103 94-95 0.4380463204758662 1103 96-97 0.39003711341149483 1103 98-99 0.26077229875956576 1103 100-101 -0.0075398950121083885 1104 1 0.35442977628256855 1104 2 0.3986876597485427 1104 3 0.4085264201380667 1104 4 0.32323337157937715 1104 5 0.3936795944629523 1104 6 0.2435915477515067 1104 7 0.3906376203309492 1104 8 0.39503406326554114 1104 9 0.46640219608606515 1104 10-11 0.5107769197176566 1104 12-13 0.40604401469133933 1104 14-15 0.5965480334355178 1104 16-17 0.43708619762692535 1104 18-19 0.5538711317995819 1104 20-21 0.49214421102883676 1104 22-23 0.595717554721297 1104 24-25 0.6139231287226181 1104 26-27 0.5642687019941732 1104 28-29 0.6323120834505005 1104 30-31 0.614863612592778 1104 32-33 0.5752483209484396 1104 34-35 0.6851309249458595 1104 36-37 0.6486734092212103 1104 38-39 1.1068625765157307 1104 40-41 0.8762840378155872 1104 42-43 0.721585229826772 1104 44-45 0.7147307630206257 1104 46-47 0.7040572232980011 1104 48-49 0.6818382397055984 1104 50-51 0.5984992057777561 1104 52-53 0.659531509460578 1104 54-55 0.8251447604422708 1104 56-57 0.818150075629994 1104 58-59 0.7674293527219902 1104 60-61 0.8412365758331859 1104 62-63 0.6556593300891862 1104 64-65 0.5906126714892892 1104 66-67 0.6112414978525464 1104 68-69 0.5234014594578227 1104 70-71 0.6961089850849476 1104 72-73 0.6009663215008061 1104 74-75 0.509837277327641 1104 76-77 0.4873197089816834 1104 78-79 0.2689789417423363 1104 80-81 0.2566934996602157 1104 82-83 0.28915174543952915 1104 84-85 0.42892529739390284 1104 86-87 0.12946043488084769 1104 88-89 0.016168903364690124 1104 90-91 0.2584171184994801 1104 92-93 0.231522489946709 1104 94-95 0.26849889849190234 1104 96-97 0.14781246033486894 1104 98-99 0.10214325020765003 1104 100-101 -0.17915314765848223 1105 1 0.1891185978461678 1105 2 0.319410204695874 1105 3 0.34539826640371984 1105 4 0.28253814170884795 1105 5 0.26822101042417756 1105 6 0.2510777437143048 1105 7 0.2800376510422282 1105 8 0.3219217485116772 1105 9 0.37216475338634325 1105 10-11 0.4618204403427626 1105 12-13 0.32171031906752745 1105 14-15 0.3686027337374611 1105 16-17 0.5062721304906859 1105 18-19 0.4321905060422253 1105 20-21 0.5597518063824438 1105 22-23 0.434859833079301 1105 24-25 0.5397763399123079 1105 26-27 0.4578605233828341 1105 28-29 0.46011236002313893 1105 30-31 0.5887510022749041 1105 32-33 0.518251516547231 1105 34-35 0.5267070082991836 1105 36-37 0.3783315686914648 1105 38-39 0.8376193701038304 1105 40-41 0.7194650851045452 1105 42-43 0.476490801187083 1105 44-45 0.5062877694490382 1105 46-47 0.6709753332452522 1105 48-49 0.6595169543485895 1105 50-51 0.5726017455324026 1105 52-53 0.49104109304894905 1105 54-55 0.6502816459332408 1105 56-57 0.6475609836103544 1105 58-59 0.6954723468068522 1105 60-61 0.7432836239784635 1105 62-63 0.593258002916933 1105 64-65 0.670110837153139 1105 66-67 0.5401354682182955 1105 68-69 0.42743116043344287 1105 70-71 0.5544358703321137 1105 72-73 0.6589017876001293 1105 74-75 0.5403251760984631 1105 76-77 0.4452677076796654 1105 78-79 0.4654045419490167 1105 80-81 0.39339677605791223 1105 82-83 0.35747590174541344 1105 84-85 0.5581368172901513 1105 86-87 0.4754095610849731 1105 88-89 0.3685048428797195 1105 90-91 0.5350365933023262 1105 92-93 0.5426608263105344 1105 94-95 0.5773752718914835 1105 96-97 0.5777436850263982 1105 98-99 0.8032181023293994 1105 100-101 0.6572498168785401 1106 1 0.2909193141667963 1106 2 0.2026851679538595 1106 3 0.2944239506475057 1106 4 0.219132299703638 1106 5 0.20464992256436432 1106 6 0.15608354444835726 1106 7 0.13049821340612056 1106 8 0.23345556657962163 1106 9 0.065031956376572 1106 10-11 0.26693683488779385 1106 12-13 0.2532494653066877 1106 14-15 0.3604587328572251 1106 16-17 0.3191619318591279 1106 18-19 0.36801082979109623 1106 20-21 0.4474456856155342 1106 22-23 0.49947579869042613 1106 24-25 0.45887116342469625 1106 26-27 0.48527027647529763 1106 28-29 0.460320428736388 1106 30-31 0.28650998635951197 1106 32-33 0.19502226190478922 1106 34-35 0.21082796987690955 1106 36-37 0.2446149254312715 1106 38-39 0.6828794237523041 1106 40-41 0.6458835242849048 1106 42-43 0.5102827723746586 1106 44-45 0.31911197627397314 1106 46-47 0.34632647564829 1106 48-49 0.3825831395452681 1106 50-51 0.2399843096362062 1106 52-53 0.3375970167803217 1106 54-55 0.27166208158983096 1106 56-57 0.4603302764165349 1106 58-59 0.29985875771323833 1106 60-61 0.4153029464583682 1106 62-63 0.3636463689639058 1106 64-65 0.4029231019422923 1106 66-67 0.2787596513848669 1106 68-69 0.2393942751404623 1106 70-71 0.4169702621256022 1106 72-73 0.3336395051478078 1106 74-75 0.4356716513137009 1106 76-77 0.5539428530708257 1106 78-79 0.7420260017832909 1106 80-81 0.7266620966157262 1106 82-83 0.6106784973950532 1106 84-85 0.6301486432508021 1106 86-87 0.7018863602647087 1106 88-89 0.6784735207295007 1106 90-91 0.6153679434838892 1106 92-93 0.5678992085697381 1106 94-95 0.8048779052453945 1106 96-97 0.9536561393847833 1106 98-99 0.9095729540988309 1106 100-101 0.7243864870899301 1107 1 0.025818170099267235 1107 2 -0.02539507143623254 1107 3 0.07554396627018534 1107 4 -0.025931500211655134 1107 5 0.0395579514808162 1107 6 -0.03233450485652867 1107 7 0.033582109593872644 1107 8 0.03039716785460911 1107 9 0.15209501673194126 1107 10-11 0.17222100763816428 1107 12-13 0.16885772663717802 1107 14-15 0.21221196471307024 1107 16-17 0.19305867075843253 1107 18-19 0.20737843550306678 1107 20-21 0.18289034363009904 1107 22-23 0.042315636292372005 1107 24-25 0.1598745086078992 1107 26-27 0.10957870309113815 1107 28-29 0.08503210430492203 1107 30-31 0.14472759738265495 1107 32-33 0.2253971394473595 1107 34-35 0.2692508192650891 1107 36-37 0.15976406079648342 1107 38-39 0.7333301681657005 1107 40-41 0.43867325374671395 1107 42-43 0.08319455020002664 1107 44-45 0.1497742104123887 1107 46-47 0.4880753956295436 1107 48-49 0.23490934916340223 1107 50-51 0.279613221432335 1107 52-53 0.444490600058721 1107 54-55 0.44815773090645905 1107 56-57 0.2549293340593053 1107 58-59 0.23422519916185536 1107 60-61 0.19028507248906834 1107 62-63 0.44564683848337694 1107 64-65 0.404119594696688 1107 66-67 0.2923404353374437 1107 68-69 0.3536414067712599 1107 70-71 0.4384224580063858 1107 72-73 0.4421468470630643 1107 74-75 0.4331289611807243 1107 76-77 0.4416603800877432 1107 78-79 0.5294044335234176 1107 80-81 0.4469223194868164 1107 82-83 0.36502236300492896 1107 84-85 0.32912582693112924 1107 86-87 0.5232222823755883 1107 88-89 0.34588000158570864 1107 90-91 0.31042000541239645 1107 92-93 0.45152178967198253 1107 94-95 0.47591581980085707 1107 96-97 0.49035265225871427 1107 98-99 0.6956655470763664 1107 100-101 0.40891631635609826 1108 1 -0.08757684714147373 1108 2 0.02962068173922816 1108 3 0.09269475744635614 1108 4 0.04925157847068817 1108 5 0.05703428507807473 1108 6 -0.019144998863943385 1108 7 -0.01683959333190188 1108 8 -0.002491776563559256 1108 9 0.1297556985720263 1108 10-11 0.2345176810232985 1108 12-13 0.1584929469196581 1108 14-15 0.22691431781503013 1108 16-17 0.28932451415879257 1108 18-19 0.12571116367312385 1108 20-21 0.15913041843341347 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-1.6878070732042332 2316 48-49 -1.4151531857376938 2316 50-51 -1.68494555019425 2316 52-53 -1.7442150740430407 2316 54-55 -1.5999671739087375 2316 56-57 -1.4495529935988003 2316 58-59 -1.3149415259251072 2316 60-61 -1.2559834870355786 2316 62-63 -1.498302989156592 2316 64-65 -1.4710274884785122 2316 66-67 -1.5327661112009743 2316 68-69 -1.6012635867138698 2316 70-71 -1.3400733774320983 2316 72-73 -1.2691223832877725 2316 74-75 -1.3752846648101062 2316 76-77 -1.2578709027736714 2316 78-79 -1.1458532906473557 2316 80-81 -1.1040922749111175 2316 82-83 -1.1571556948142465 2316 84-85 -0.8535813918839175 2316 86-87 -0.9451328518017412 2316 88-89 -1.121107946508058 2316 90-91 -1.110391380018946 2316 92-93 -1.1897298813500186 2316 94-95 -1.2376423027560648 2316 96-97 -1.2451055280738679 2316 98-99 -1.5143912858020947 2316 100-101 -0.822307122552786 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11872.0 3 3241.0 4 3344.0 5 3601.0 6 4126.0 7 4600.0 8 5742.0 9 6692.0 10 7817.0 11 9040.0 12 10919.0 13 13636.0 14 13618.0 15 14003.0 16 16588.0 17 20408.0 18 18848.0 19 14791.0 20 14817.0 21 15924.0 22 17421.0 23 19532.0 24 22002.0 25 24960.0 26 28530.0 27 33297.0 28 38596.0 29 45340.0 30 53968.0 31 65981.0 32 80902.0 33 103553.0 34 140224.0 35 199010.0 36 304127.0 37 463044.0 38 408903.0 39 55665.0 40 18.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.39123687395275 18.901983818381094 14.745333705881386 22.96144560178477 2 14.87579453820869 20.182480238076312 40.984302793801994 23.957422429913002 3 19.532165410259335 24.43552400999596 29.58286239660995 26.44944818313476 4 12.752643926714624 16.444223766106315 37.34391725080886 33.459215056370205 5 13.85158818175296 37.47347411895178 33.99500729974959 14.679930399545679 6 31.838165801950026 35.74452340131761 17.819696542529424 14.597614254202945 7 28.677167203985803 32.54422411433023 20.64911836767829 18.12949031400568 8 25.972965934693804 35.211872220660304 19.81219793541243 19.002963909233465 9 26.2934061444296 17.528260800087068 20.531545764486278 35.646787290997054 10-11 24.99108810608166 26.629732237262143 27.235589883170526 21.143589773485676 12-13 26.03713811431161 23.897673400399356 27.760104647003107 22.305083838285924 14-15 22.761248575162845 25.43693026756817 26.309916305189702 25.491904852079283 16-17 22.17856078142442 28.717645278509167 26.839078387889025 22.26471555217739 18-19 22.018923916303592 27.534278787779325 28.967597344113273 21.479199951803803 20-21 22.996960601866494 26.480389056784386 28.68786210566876 21.834788235680357 22-23 22.54510667232291 26.388731691006655 28.7293193586096 22.336842278060832 24-25 22.55259712482014 26.586037186350893 28.79943740844924 22.06192828037973 26-27 22.272184313242654 26.684767934403425 28.964426040768892 22.07862171158503 28-29 21.9654993355786 27.148828665679392 28.922224679842195 21.96344731889981 30-31 22.17393867937263 26.66416417767643 28.776665168484968 22.385231974465974 32-33 22.06524002443401 26.775353960547882 28.77027275719866 22.389133257819445 34-35 22.718983887151854 26.752638210827058 28.469717622125245 22.058660279895843 36-37 22.5851123616197 26.5127752494626 28.64659093420271 22.255521454714994 38-39 22.246216560222624 26.919214353518395 28.76180913795161 22.072759948307375 40-41 22.37559860315464 26.79052560139667 28.649102587923252 22.18477320752543 42-43 22.18839816570317 26.79211844128524 28.34179644728996 22.677686945721632 44-45 22.401313894100863 26.81226884574611 28.215711189371355 22.570706070781668 46-47 22.573732128424712 27.12599746249138 27.908977221993446 22.391293187090465 48-49 22.590260886671075 27.01098562554934 27.930162731633086 22.468590756146497 50-51 22.238378390556786 27.254933648985197 28.05676534253484 22.44992261792318 52-53 22.586117995736345 27.36942562004957 27.63102934294677 22.413427041267312 54-55 22.230961213463928 27.084011140661463 27.820895108566862 22.864132537307743 56-57 22.452087833900883 27.354522638689293 27.659325233701114 22.53406429370871 58-59 22.778746983590473 26.926161239186282 27.73756620079837 22.557525576424872 60-61 22.481474861166955 27.002967744336853 28.23490408390532 22.28065331059087 62-63 22.297163067866826 27.06806873664004 28.184461789664805 22.45030640582833 64-65 22.150226234933378 27.200445060851415 27.920701005503034 22.728627698712174 66-67 22.207475014424023 27.26061804377927 27.615612656570825 22.916294285225877 68-69 22.161259707391483 27.096558209679657 27.928143634724726 22.814038448204133 70-71 22.397868326522353 26.980974970213467 27.824466274539628 22.79669042872455 72-73 22.369247181463937 26.820820444336213 27.969033475789338 22.84089889841051 74-75 22.104000140396092 27.387616501134325 27.649364220185273 22.859019138284303 76-77 22.242985512244108 26.953455668991904 27.6710202426554 23.132538576108587 78-79 22.277844435877554 27.043311401134005 27.739135331343697 22.939708831644744 80-81 22.328975745151045 27.164742128988134 27.671825574036912 22.834456551823912 82-83 22.394608180780857 26.97204886877671 27.805401696250108 22.827941254192325 84-85 22.23611619529925 26.991298998637557 27.70678686770449 23.0657979383587 86-87 22.192380914010464 26.99340191563437 27.807088397348462 23.00712877300671 88-89 22.184219088678645 26.840321418560286 27.638408885411348 23.337050607349717 90-91 22.13558542777206 26.840167030770868 27.819099253223477 23.205148288233595 92-93 22.367620497068827 26.675089529937495 27.885628754970515 23.071661218023166 94-95 22.190932357557568 26.585585062449248 27.907610132282674 23.31587244771051 96-97 22.027252478679888 26.537149864799282 28.059587984469253 23.376009672051584 98-99 21.989429994022334 26.641220596713733 27.652592463719632 23.716756945544297 100-101 22.089902648175 25.95590151437857 28.310727806758113 23.64346803068832 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2635.0 1 1515.0 2 432.5 3 457.5 4 411.0 5 410.0 6 582.0 7 1120.5 8 1833.5 9 2159.0 10 2111.0 11 2093.0 12 2234.5 13 2420.5 14 2694.5 15 2863.5 16 2933.0 17 3098.0 18 3224.5 19 3324.5 20 3629.5 21 4172.0 22 4823.5 23 5778.0 24 6973.5 25 8600.0 26 10962.5 27 13844.5 28 16596.0 29 20160.5 30 24808.5 31 29352.0 32 33972.0 33 38372.0 34 43421.5 35 48411.0 36 53435.0 37 58934.5 38 63381.0 39 66440.0 40 68850.5 41 73030.5 42 77554.0 43 80859.5 44 83980.5 45 87406.5 46 88934.0 47 93593.5 48 104966.0 49 104944.5 50 94505.5 51 87972.0 52 83307.0 53 80152.5 54 76790.0 55 70081.5 56 61726.0 57 54797.0 58 49082.5 59 42500.5 60 35389.5 61 28900.5 62 23299.5 63 18181.0 64 13590.5 65 10111.5 66 7603.0 67 6010.5 68 4548.5 69 3183.0 70 2385.5 71 1805.5 72 1402.5 73 1136.0 74 840.0 75 622.5 76 454.5 77 280.0 78 175.5 79 115.5 80 81.5 81 58.5 82 43.5 83 34.0 84 25.0 85 16.0 86 14.0 87 10.5 88 10.5 89 10.0 90 4.5 91 3.0 92 2.5 93 3.5 94 3.0 95 2.5 96 2.5 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28869625221029027 2 0.17889334540906543 3 0.1795833872428516 4 0.19937896234959243 5 0.21227411911847158 6 0.10898348212360376 7 0.12429378531073447 8 0.13701643162116703 9 0.14240738344762152 10-11 0.12828308966231078 12-13 0.11745805839479019 14-15 0.1330271272695907 16-17 0.10866002501401648 18-19 0.13647733643852158 20-21 0.16250485185664382 22-23 0.1662138267132445 24-25 0.190192780437314 26-27 0.17013843964290334 28-29 0.16837020744382628 30-31 0.15834303704662095 32-33 0.16793893129770993 34-35 0.3489239660154397 36-37 0.46746021477552074 38-39 0.4335834734980808 40-41 0.4219174537456333 42-43 0.446349247423125 44-45 0.4505541898477595 46-47 0.4541553456678311 48-49 0.4666623539052055 50-51 0.4602579031353776 52-53 0.4666407900978997 54-55 0.448484064346401 56-57 0.40973390261784626 58-59 0.3988873075430198 60-61 0.3532582912839091 62-63 0.46282399620476994 64-65 0.46150860395911497 66-67 0.4708888601371458 68-69 0.48216673135808863 70-71 0.4756760253590374 72-73 0.4680640013800837 74-75 0.47196705050243676 76-77 0.44878595764868245 78-79 0.4342950791391728 80-81 0.4315780394186398 82-83 0.44481821710441194 84-85 0.44615517315737263 86-87 0.44759994824686244 88-89 0.45529822745503945 90-91 0.47360589985767887 92-93 0.4783715012722647 94-95 0.4772501832923621 96-97 0.4752663130202268 98-99 0.47196705050243676 100-101 0.453939707594773 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2318700.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.60694056801747 #Duplication Level Percentage of deduplicated Percentage of total 1 77.08724879352707 44.4076055980069 2 13.166147036194046 15.169228996476194 3 4.286075426638248 7.407230771171689 4 1.902610435648179 4.38414265161898 5 0.9600588350128728 2.7653026125193327 6 0.5784230102874947 1.9992707986083282 7 0.3894477440632121 1.570442513261856 8 0.2632183509105387 1.2130563117851976 9 0.19963787475135347 1.035047446733388 >10 1.0508365237110395 10.948883713638315 >50 0.07715739585171004 3.057032246198228 >100 0.036732833613895 3.8864677355672783 >500 0.0013532286320939633 0.5354667156396791 >1k 8.269730529460772E-4 0.8788600825358825 >5k 2.255381053489301E-4 0.7419618062388379 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6463 0.27873377323500237 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5644 0.2434122568680726 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5025 0.21671626342347006 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3412 0.14715142105490145 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3337 0.1439168499590288 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 8.625522922327166E-5 0.0 0.0 0.0 0.0 12-13 8.625522922327166E-5 0.0 0.0 0.0 0.0 14-15 8.625522922327166E-5 0.0 0.0 0.0 0.0 16-17 8.625522922327166E-5 0.0 0.0 0.0 0.0 18-19 1.0781903652908957E-4 0.0 0.0 0.0 0.0 20-21 1.2938284383490748E-4 0.0 0.0 4.312761461163583E-5 0.0 22-23 1.2938284383490748E-4 0.0 0.0 4.312761461163583E-5 0.0 24-25 1.2938284383490748E-4 0.0 0.0 1.509466511407254E-4 0.0 26-27 1.2938284383490748E-4 0.0 0.0 3.8814853150472245E-4 0.0 28-29 2.1563807305817915E-4 0.0 0.0 0.0016172855479363439 0.0 30-31 2.5876568766981497E-4 0.0 0.0 0.00547720705567775 0.0 32-33 2.5876568766981497E-4 0.0 0.0 0.014641825160650365 0.0 34-35 2.5876568766981497E-4 0.0 0.0 0.02842109802906801 0.0 36-37 2.5876568766981497E-4 0.0 0.0 0.0509121490490361 0.0 38-39 2.5876568766981497E-4 0.0 0.0 0.08409884849268986 0.0 40-41 2.5876568766981497E-4 0.0 0.0 0.12136110751714323 0.0 42-43 3.018933022814508E-4 0.0 0.0 0.16256954327856127 0.0 44-45 3.4502091689308666E-4 0.0 0.0 0.21315823521801008 0.0 46-47 3.4502091689308666E-4 0.0 0.0 0.2789709751153664 0.0 48-49 3.4502091689308666E-4 0.0 0.0 0.34691853193599864 0.0 50-51 3.4502091689308666E-4 0.0 0.0 0.42726527795747615 0.0 52-53 3.4502091689308666E-4 0.0 0.0 0.5045283995342218 0.0 54-55 3.4502091689308666E-4 0.0 0.0 0.5947944969163755 0.0 56-57 3.4502091689308666E-4 0.0 0.0 0.7035623409669212 0.0 58-59 3.4502091689308666E-4 0.0 0.0 0.8290636994867814 0.0 60-61 3.4502091689308666E-4 0.0 0.0 0.9668564281709578 0.0 62-63 3.4502091689308666E-4 0.0 0.0 1.109544141113555 0.0 64-65 3.4502091689308666E-4 0.0 0.0 1.2601457713373874 0.0 66-67 3.8814853150472245E-4 0.0 0.0 1.435006684780265 0.0 68-69 3.8814853150472245E-4 0.0 0.0 1.638914046664079 0.0 70-71 3.8814853150472245E-4 0.0 0.0 1.867598223142278 0.0 72-73 4.097123388105404E-4 0.0 0.0 2.1005951610816407 0.0 74-75 4.312761461163583E-4 0.0 0.0 2.3406650278173116 0.0 76-77 4.312761461163583E-4 0.0 0.0 2.606352697632294 0.0 78-79 4.528399534221762E-4 0.0 0.0 2.92183119851641 4.312761461163583E-5 80-81 4.7440376072799414E-4 0.0 0.0 3.2593047828524604 4.312761461163583E-5 82-83 4.7440376072799414E-4 0.0 0.0 3.6104929486350112 4.312761461163583E-5 84-85 4.7440376072799414E-4 0.0 0.0 3.9722473799974125 4.312761461163583E-5 86-87 4.7440376072799414E-4 0.0 0.0 4.3559753310044425 4.312761461163583E-5 88-89 4.7440376072799414E-4 0.0 0.0 4.777892784750075 4.312761461163583E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9705 0.0 23.715221 1 TACATGG 9670 0.0 23.241632 2 GTATCAA 15515 0.0 22.63398 1 GAGTACT 6705 0.0 21.518986 12-13 ACATGGG 9925 0.0 21.497936 3 GTACTTT 7025 0.0 20.408047 14-15 TCAACGC 17640 0.0 19.729326 4 AGTACTT 7030 0.0 19.71532 12-13 ATCAACG 17730 0.0 19.65592 3 AGAGTAC 12710 0.0 19.591173 10-11 CAACGCA 17645 0.0 19.589376 5 TATCAAC 17955 0.0 19.356789 2 AACGCAG 18020 0.0 19.223541 6 GGTATCA 11880 0.0 19.133747 1 ACCGTGC 325 5.0386006E-10 18.955307 8 TACCTGG 2025 0.0 17.561071 2 ACGCAGA 19570 0.0 17.507263 7 GTACACG 380 2.1100277E-10 17.483381 1 CATGGGA 5355 0.0 17.443014 4 CGCAGAG 19710 0.0 17.262697 8 >>END_MODULE