FastQCFastQC Report
Fri 27 May 2016
SRR939287_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939287_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2318700
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64950.2801138569025747No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT61760.2663561478414629No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54940.23694311467632725No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35790.15435373269504463No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT32030.13813774960106956No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG95150.023.7106251
TACATGG97150.022.6634562
ACATGGG97000.022.209313
GAGTACT69800.021.21229212-13
GTATCAA159850.020.0092161
CATGGGG49350.019.6152554
GTACTTT76800.019.18619314-15
AGTACTT74250.018.95032512-13
AGAGTAC129900.018.70423110-11
GGTATCA128750.018.2622011
GTAAGCG1902.1100277E-1017.48217294-95
ATCAACG183100.017.2080123
TCAACGC183900.017.184764
CAACGCA184500.017.1288745
TATCAAC186950.016.9551622
AACGCAG187650.016.8160516
GAGTACA82400.016.6933731
CATGGGA54300.016.5163064
ACTTTTT90150.016.2918116-17
TACTTTT86800.016.23772414-15
Produced by FastQC (version 0.11.5)