##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939287_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2318700 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.2914270927675 33.0 31.0 34.0 31.0 34.0 2 32.49789537240695 34.0 31.0 34.0 31.0 34.0 3 32.59032043817656 34.0 31.0 34.0 31.0 34.0 4 36.117188942079615 37.0 35.0 37.0 35.0 37.0 5 36.06002889550179 37.0 35.0 37.0 35.0 37.0 6 36.08900245827403 37.0 35.0 37.0 35.0 37.0 7 36.03122568680726 37.0 35.0 37.0 35.0 37.0 8 35.99982748954155 37.0 35.0 37.0 35.0 37.0 9 37.70126191400354 39.0 37.0 39.0 35.0 39.0 10-11 37.61853646439815 39.0 37.0 39.0 35.0 39.0 12-13 37.5494725492733 39.0 37.0 39.0 35.0 39.0 14-15 38.83579290119464 40.0 38.0 41.0 35.0 41.0 16-17 38.84000107819037 40.0 38.0 41.0 35.0 41.0 18-19 38.94397830680985 40.0 38.0 41.0 35.0 41.0 20-21 38.924406995299094 40.0 38.0 41.0 35.0 41.0 22-23 38.846438737223444 40.0 38.0 41.0 35.0 41.0 24-25 38.78559106395825 40.0 38.0 41.0 34.0 41.0 26-27 38.6346390218657 40.0 38.0 41.0 34.0 41.0 28-29 38.4751856643809 40.0 38.0 41.0 34.0 41.0 30-31 38.29720533057316 40.0 38.0 41.0 34.0 41.0 32-33 38.259974123431235 40.0 38.0 41.0 34.0 41.0 34-35 38.17482425497046 40.0 38.0 41.0 33.5 41.0 36-37 38.028382714452064 40.0 38.0 41.0 33.0 41.0 38-39 37.91192068831673 40.0 37.0 41.0 33.0 41.0 40-41 37.745591710872475 40.0 37.0 41.0 33.0 41.0 42-43 37.57452969336266 40.0 37.0 41.0 33.0 41.0 44-45 37.336963169017125 40.0 37.0 41.0 32.0 41.0 46-47 37.049921723379484 40.0 36.5 41.0 31.0 41.0 48-49 36.75863156078837 40.0 36.0 41.0 31.0 41.0 50-51 36.523609565704916 39.0 36.0 41.0 30.0 41.0 52-53 36.283356406607155 39.0 35.0 41.0 30.0 41.0 54-55 36.04102751541812 39.0 35.0 40.0 29.0 41.0 56-57 35.73106891792815 38.5 35.0 40.0 28.5 41.0 58-59 35.42116099538535 38.0 34.5 40.0 28.0 41.0 60-61 35.07311446068918 38.0 34.0 40.0 28.0 41.0 62-63 34.733583689136154 37.5 34.0 40.0 27.0 41.0 64-65 34.42286518307672 37.0 34.0 40.0 26.0 41.0 66-67 34.00124746625264 36.5 33.0 39.0 26.0 40.5 68-69 33.529484625005395 36.0 33.0 39.0 25.5 40.0 70-71 33.04217018156726 35.0 32.0 38.5 24.5 40.0 72-73 32.54630396342779 35.0 32.0 37.5 23.5 39.5 74-75 32.01705826540734 35.0 31.0 37.0 22.0 39.0 76-77 30.06438262819683 33.0 29.0 35.0 19.5 37.5 78-79 31.024014534006124 34.0 30.5 36.0 20.0 38.0 80-81 31.023780135420708 34.0 31.0 36.0 20.0 37.0 82-83 30.652370724975203 34.0 31.0 35.0 18.5 37.0 84-85 30.19433842239186 34.0 30.0 35.0 16.5 36.5 86-87 29.5866407900979 34.0 29.5 35.0 8.5 36.0 88-89 28.992960063828868 34.0 29.0 35.0 2.0 35.5 90-91 28.497625177901412 33.5 29.0 35.0 2.0 35.0 92-93 28.121303963427785 33.0 28.0 35.0 2.0 35.0 94-95 27.584334756544614 33.0 27.0 35.0 2.0 35.0 96-97 26.830844654332168 33.0 25.5 34.5 2.0 35.0 98-99 25.814915254237288 32.0 24.5 34.0 2.0 35.0 100-101 21.495687238538835 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.27368830487110074 1101 2 0.3386356576386902 1101 3 0.3092261352149279 1101 4 0.1492333503039731 1101 5 0.2384426322777884 1101 6 0.26464152876381064 1101 7 0.2796434325234216 1101 8 0.2807245568387131 1101 9 0.3820273314862419 1101 10-11 0.3546664913232149 1101 12-13 0.35036090486524785 1101 14-15 0.4005240122085496 1101 16-17 0.39194255625668006 1101 18-19 0.42943441083841094 1101 20-21 0.45118890767096786 1101 22-23 0.3941505738422464 1101 24-25 0.4701655463018142 1101 26-27 0.44935353864918426 1101 28-29 0.4204576693889237 1101 30-31 0.4161504338653401 1101 32-33 0.5428087028381015 1101 34-35 0.5555822369449217 1101 36-37 0.5656033764002544 1101 38-39 0.5875658217522499 1101 40-41 0.5387179008141842 1101 42-43 0.5412514091901528 1101 44-45 0.7293376387183486 1101 46-47 0.8595150628245278 1101 48-49 0.7548314737437423 1101 50-51 0.7165405629796098 1101 52-53 0.7069342965245937 1101 54-55 0.6123389010479272 1101 56-57 0.5442726300196199 1101 58-59 0.5480254128952069 1101 60-61 0.5020832702089351 1101 62-63 0.582888138839941 1101 64-65 0.5068899637587094 1101 66-67 0.723491249991774 1101 68-69 0.8210551766270413 1101 70-71 0.6637760089390667 1101 72-73 0.6631033281812364 1101 74-75 0.7735957581624362 1101 76-77 0.688682797694387 1101 78-79 0.9130464670760112 1101 80-81 1.0255807174817626 1101 82-83 0.7926498469572145 1101 84-85 0.9142230517211161 1101 86-87 0.9556368847875021 1101 88-89 1.071755850038464 1101 90-91 1.1479768183447376 1101 92-93 1.0968027014833517 1101 94-95 0.9189842891544302 1101 96-97 0.9393917968278629 1101 98-99 0.8521872019620602 1101 100-101 0.5536695128264668 1102 1 0.23690586396420343 1102 2 0.19274185094709395 1102 3 0.23723321216631632 1102 4 0.16265599599073965 1102 5 0.18582488524673124 1102 6 0.20969547030227886 1102 7 0.24442388809170978 1102 8 0.21699295677291275 1102 9 0.26398612455651715 1102 10-11 0.24226621136816107 1102 12-13 0.3044102148764338 1102 14-15 0.4295958385248895 1102 16-17 0.3156077506497681 1102 18-19 0.3274975903358808 1102 20-21 0.32778631055807494 1102 22-23 0.4471112936947961 1102 24-25 0.4073645737089677 1102 26-27 0.4224807118560463 1102 28-29 0.346769749560913 1102 30-31 0.3600433566351171 1102 32-33 0.4518557263638101 1102 34-35 0.5036155805953229 1102 36-37 0.5604762115586013 1102 38-39 0.4209388897417341 1102 40-41 0.5082797600057205 1102 42-43 0.6012611964633479 1102 44-45 0.605968153818921 1102 46-47 0.6051895620981469 1102 48-49 0.4656750614067562 1102 50-51 0.5637510506981087 1102 52-53 0.5496945027544342 1102 54-55 0.4864675270095802 1102 56-57 0.4472503590934025 1102 58-59 0.494691396404626 1102 60-61 0.49570376659934823 1102 62-63 0.6429390549677478 1102 64-65 0.47305276710144284 1102 66-67 0.40714448216206733 1102 68-69 0.40165344112879353 1102 70-71 0.18457047899028822 1102 72-73 0.30210386913024223 1102 74-75 0.3734095629579244 1102 76-77 0.33668742085648873 1102 78-79 0.3345855088155254 1102 80-81 0.5485835978957816 1102 82-83 0.5272826429664583 1102 84-85 0.5465788428032639 1102 86-87 0.35682725143381333 1102 88-89 0.4843051000991103 1102 90-91 0.5560778782144098 1102 92-93 0.526240574605719 1102 94-95 0.6124952015457907 1102 96-97 0.35371864992674773 1102 98-99 0.18003750129647145 1102 100-101 0.1825603721825111 1103 1 0.13945107049114114 1103 2 0.16869978593928892 1103 3 0.07425540360598148 1103 4 0.07066472403923285 1103 5 0.10475904989272067 1103 6 0.09598987303268558 1103 7 0.1666182193582415 1103 8 0.14815331997649395 1103 9 0.1928717607755317 1103 10-11 0.17605093296884178 1103 12-13 0.1991795361715205 1103 14-15 0.25488283003321044 1103 16-17 0.17021616117281013 1103 18-19 0.19643262705234577 1103 20-21 0.21746755962002595 1103 22-23 0.2774400000057895 1103 24-25 0.21276732568799872 1103 26-27 0.2940080928035016 1103 28-29 0.257617755479977 1103 30-31 0.29651187512048693 1103 32-33 0.3866373622119639 1103 34-35 0.3371234427696308 1103 36-37 0.3966125629901498 1103 38-39 0.3557799640774775 1103 40-41 0.3244862029541835 1103 42-43 0.32028605643866825 1103 44-45 0.26206337565299265 1103 46-47 0.25669803747570086 1103 48-49 0.24237544966171498 1103 50-51 0.24239675279057593 1103 52-53 0.02693277489824908 1103 54-55 0.1287859860335132 1103 56-57 0.2195511518054616 1103 58-59 0.1617420035722219 1103 60-61 0.3520424337910981 1103 62-63 0.24628422551031548 1103 64-65 0.34858949205454337 1103 66-67 0.1667925650069293 1103 68-69 0.14866614076982643 1103 70-71 0.11560278552948233 1103 72-73 0.07670130533484354 1103 74-75 0.12544091343249164 1103 76-77 0.24979076166667724 1103 78-79 0.2226545749611546 1103 80-81 0.46487515951238123 1103 82-83 0.41701736371802056 1103 84-85 0.3334725874417508 1103 86-87 0.28035011322028325 1103 88-89 0.49213652245064665 1103 90-91 0.41116569800609426 1103 92-93 0.28006586584283255 1103 94-95 0.03064760091000096 1103 96-97 -0.07716207699448319 1103 98-99 -0.20299443989109633 1103 100-101 -0.27109413093697654 1104 1 0.17445225872204873 1104 2 0.15437426051426684 1104 3 0.14504815388434622 1104 4 0.07904953996135333 1104 5 0.09621446809214973 1104 6 0.029998109653519123 1104 7 0.08579048079752027 1104 8 0.10893902987547932 1104 9 0.16303203398853583 1104 10-11 0.19405819408325442 1104 12-13 0.13931606256760887 1104 14-15 0.11950038434248711 1104 16-17 0.11614075167818072 1104 18-19 0.14927098581619447 1104 20-21 0.22059055976510678 1104 22-23 0.19032620842771308 1104 24-25 0.18376981874774856 1104 26-27 0.19442010185720449 1104 28-29 0.11679592692166807 1104 30-31 0.14825492530476936 1104 32-33 0.18793222237899698 1104 34-35 0.15966182440804033 1104 36-37 0.2273548557328624 1104 38-39 0.1938790527444283 1104 40-41 0.29450095125839226 1104 42-43 0.33158226059720874 1104 44-45 0.24183941860376024 1104 46-47 0.2603475084681719 1104 48-49 0.057391039073195316 1104 50-51 0.15463299445627143 1104 52-53 0.16911105581739605 1104 54-55 0.1276560540964482 1104 56-57 0.1593154103538268 1104 58-59 0.12649560364484813 1104 60-61 0.1729488893116482 1104 62-63 0.18538205118126427 1104 64-65 0.15141125118812226 1104 66-67 0.13638253188777583 1104 68-69 0.24279316487889702 1104 70-71 0.1522964239993314 1104 72-73 0.23144078904628884 1104 74-75 0.23272125315082093 1104 76-77 0.2851214948363001 1104 78-79 0.4081779172455313 1104 80-81 0.3661687735945627 1104 82-83 0.3018617677734916 1104 84-85 0.34910605692468977 1104 86-87 0.24681351971362986 1104 88-89 0.324592626886794 1104 90-91 0.44208395573026493 1104 92-93 0.6075710081679695 1104 94-95 0.6549238703811895 1104 96-97 0.5682079612514244 1104 98-99 0.4931620114016688 1104 100-101 0.45898186243741534 1105 1 0.15766178452359014 1105 2 0.06986604952347619 1105 3 0.0833135566833505 1105 4 0.11012482409418567 1105 5 0.06827062268880724 1105 6 0.17292576747336597 1105 7 0.10560332162111052 1105 8 0.04765526403917164 1105 9 0.14831842097721903 1105 10-11 0.09070392746055234 1105 12-13 0.0750814685746164 1105 14-15 0.14956921097900988 1105 16-17 0.13792148851798913 1105 18-19 0.12749388471318923 1105 20-21 0.1437369648982525 1105 22-23 0.08498725723941192 1105 24-25 0.23623660201463537 1105 26-27 0.17485545353774512 1105 28-29 0.19432010136346634 1105 30-31 0.18591217626855894 1105 32-33 0.13571416628342803 1105 34-35 0.2736409971966438 1105 36-37 0.0744093528612737 1105 38-39 0.1586459827667568 1105 40-41 0.294838540114732 1105 42-43 0.32163060734213644 1105 44-45 0.13677378927695827 1105 46-47 0.14964777028399823 1105 48-49 0.2674450917862501 1105 50-51 0.21156039905774549 1105 52-53 0.3719774707199832 1105 54-55 0.35351871820184755 1105 56-57 0.3820436415935333 1105 58-59 0.2152738805879082 1105 60-61 0.1935850106562924 1105 62-63 0.3181577694210702 1105 64-65 0.22904214519869726 1105 66-67 0.25052071469518467 1105 68-69 0.33707370175317664 1105 70-71 0.28222290084631396 1105 72-73 0.2694222621869642 1105 74-75 0.35301110264414604 1105 76-77 0.5000213431191405 1105 78-79 0.37373872109410655 1105 80-81 0.3443534972550424 1105 82-83 0.40098542688167527 1105 84-85 0.40996080581718175 1105 86-87 0.34900574069114754 1105 88-89 0.42249200509025187 1105 90-91 0.49262272915242633 1105 92-93 0.4274774585211283 1105 94-95 0.5996059209196432 1105 96-97 0.6898335162894185 1105 98-99 0.49350141812641724 1105 100-101 0.18350133565764537 1106 1 0.07775029987624293 1106 2 0.08265023460850784 1106 3 0.16886717966848863 1106 4 0.10688681142143963 1106 5 0.08709575287533511 1106 6 0.1251253812414106 1106 7 0.11891951775020715 1106 8 0.0780379311193542 1106 9 0.05177735929678562 1106 10-11 0.10538128593705665 1106 12-13 0.12226712007554141 1106 14-15 0.14676789070493612 1106 16-17 0.14942985469393477 1106 18-19 0.1767975044236465 1106 20-21 0.12557140749476048 1106 22-23 0.16864720303473746 1106 24-25 0.2397377989387266 1106 26-27 0.18125046811061196 1106 28-29 0.16964617476124744 1106 30-31 0.08345239096323098 1106 32-33 0.09463555822583913 1106 34-35 0.3205723312969653 1106 36-37 0.25714476825206845 1106 38-39 0.2143621492322083 1106 40-41 0.2024413205173019 1106 42-43 0.11540216865146391 1106 44-45 0.21086751148618532 1106 46-47 0.17482801421509464 1106 48-49 0.246648699465986 1106 50-51 0.34837590588995937 1106 52-53 0.442414221559261 1106 54-55 0.4014166397918828 1106 56-57 0.39546160498673544 1106 58-59 0.3805292065927901 1106 60-61 0.488548558831404 1106 62-63 0.6331729952144158 1106 64-65 0.46118556731540394 1106 66-67 0.392341256487974 1106 68-69 0.41052498032282614 1106 70-71 0.2812082509952276 1106 72-73 0.3728010764189875 1106 74-75 0.5277598029955755 1106 76-77 0.41572530154327936 1106 78-79 0.5356212179912134 1106 80-81 0.5769690791733559 1106 82-83 0.5360756259283654 1106 84-85 0.5307801108858143 1106 86-87 0.643479899161516 1106 88-89 0.8647018016302859 1106 90-91 0.7604720523396651 1106 92-93 0.7816316687739082 1106 94-95 0.9749432390963051 1106 96-97 0.8973591349386609 1106 98-99 0.7526869213091096 1106 100-101 0.4949336484366107 1107 1 -0.0011205801714524455 1107 2 0.02764436942924675 1107 3 0.07075973217809661 1107 4 -0.06350413123902143 1107 5 0.03405759764823557 1107 6 0.024088681486524877 1107 7 -0.051183458053515096 1107 8 0.010591516316260652 1107 9 0.09446987610613888 1107 10-11 0.08376765698063338 1107 12-13 0.03882309749512558 1107 14-15 0.029907316819411278 1107 16-17 0.1215519930709732 1107 18-19 0.030333270193310113 1107 20-21 0.12927807491917065 1107 22-23 0.08251355094180468 1107 24-25 0.1007450650481303 1107 26-27 0.13047742966551112 1107 28-29 0.11127529290183702 1107 30-31 0.039667411810313524 1107 32-33 0.08704601054548533 1107 34-35 0.12327536747837797 1107 36-37 0.04133163089893088 1107 38-39 0.0404207277934745 1107 40-41 0.011614552415103674 1107 42-43 0.049751637219124234 1107 44-45 0.0034702531976549267 1107 46-47 0.036053257885590995 1107 48-49 -0.07561825191281457 1107 50-51 0.04687012528903978 1107 52-53 0.05276607700982083 1107 54-55 0.042436870131034254 1107 56-57 0.08175775811187691 1107 58-59 0.07109375321947908 1107 60-61 0.04446132890283394 1107 62-63 -0.049176486321279356 1107 64-65 -0.055065966203457606 1107 66-67 0.0874620681196987 1107 68-69 0.09837415205577571 1107 70-71 0.04843920556340464 1107 72-73 0.034687270772963075 1107 74-75 0.04746074083306695 1107 76-77 0.09187157650128697 1107 78-79 0.13230914144313743 1107 80-81 0.0020024385127044297 1107 82-83 0.17206139464444803 1107 84-85 0.05733780657121201 1107 86-87 0.24203626981796234 1107 88-89 0.5807086968155026 1107 90-91 0.5001372365173573 1107 92-93 0.35090524281829616 1107 94-95 0.5375031805417727 1107 96-97 0.4614748602365921 1107 98-99 0.5130969708565729 1107 100-101 0.41674082544793123 1108 1 0.03794176239297542 1108 2 0.09297214136709187 1108 3 0.030228143571385147 1108 4 0.08332997329581815 1108 5 0.0551099515379434 1108 6 0.10860275492171212 1108 7 0.06949568535772244 1108 8 0.06001662342283254 1108 9 0.07565345088801223 1108 10-11 0.07314704159004037 1108 12-13 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90-91 -0.7075138383758564 2316 92-93 -0.48939053109756614 2316 94-95 -0.29571771889715137 2316 96-97 -0.19950000735077822 2316 98-99 0.014916209184800522 2316 100-101 0.5197644687472867 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 6.0 4 24.0 5 50.0 6 95.0 7 224.0 8 592.0 9 1052.0 10 1801.0 11 2856.0 12 4089.0 13 5780.0 14 7191.0 15 9794.0 16 12785.0 17 17937.0 18 17451.0 19 12610.0 20 11951.0 21 13080.0 22 13832.0 23 15733.0 24 17957.0 25 21277.0 26 25869.0 27 31108.0 28 38065.0 29 46619.0 30 56916.0 31 69028.0 32 85918.0 33 110408.0 34 150219.0 35 213351.0 36 337510.0 37 533884.0 38 405463.0 39 26169.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.54592686563226 19.59133923964237 15.218874121914835 22.643859772810533 2 15.418726010264372 20.791607366196576 40.055418984775955 23.7342476387631 3 19.59347910467072 24.553327295467287 29.934704791477984 25.918488808384012 4 13.18514684952775 17.13235864924311 36.900806486393236 32.78168801483589 5 13.766636477336439 37.59736058998577 34.05606589899512 14.579937033682667 6 31.809030922499677 36.2190451546125 17.43395005822228 14.537973864665545 7 28.738258506921984 32.65748048475439 20.543494199335836 18.060766808987793 8 26.055462112390565 35.64605166688231 19.496657609867597 18.801828610859534 9 26.062405658343035 17.596325527235088 20.44141113555009 35.89985767887178 10-11 25.065985250355805 26.912170612843404 27.01175227498167 21.01009186181912 12-13 25.9937033682667 24.044766463966877 27.72135248199422 22.2401776857722 14-15 22.66568075600285 25.581017726337336 26.267704934965906 25.485596582693905 16-17 22.08340772904607 28.93684860623406 26.785842129278613 22.193901535441256 18-19 22.016412231435968 27.599137015501157 28.958197436276183 21.426253316786685 20-21 22.939022499465757 26.490431381901796 28.795581316164515 21.774964802467935 22-23 22.516884461120455 26.445616077974726 28.804459395350847 22.233040065553975 24-25 22.553564497347654 26.641307629275023 28.861387846638202 21.94374002673912 26-27 22.271033140150205 26.791263890338456 28.970913849065294 21.96678912044604 28-29 21.98067882865399 27.221891577176866 28.958058394790182 21.839371199378963 30-31 22.291348600508908 26.650342864536164 28.83624444731962 22.222064087635314 32-33 22.12101608660025 26.812287057402855 28.781299866304394 22.2853969896925 34-35 22.710683058559734 26.80456633155307 28.58088905843078 21.90386155145641 36-37 22.587819511546055 26.535079821411895 28.77321066309772 22.103890003944326 38-39 22.24990851304204 26.92550125903737 28.857110633771182 21.967479594149406 40-41 22.38856437756255 26.851790153128086 28.71324663931844 22.046398829990927 42-43 22.2291466033323 26.813463067004385 28.383330479721604 22.57405984994171 44-45 22.38735066039036 26.862099400668445 28.282420089305642 22.468129849635556 46-47 22.642970892063325 27.195699309633962 27.93447614447335 22.22685365382936 48-49 22.637209273239435 27.085058892001037 27.939333077010826 22.338398757748706 50-51 22.293246215551818 27.2624099711045 28.064475783844394 22.37986802949929 52-53 22.69527006308279 27.3908989480739 27.57432077212264 22.33951021672067 54-55 22.303294949756328 27.16890930262647 27.753051278733775 22.774744468883426 56-57 22.490118841297885 27.369057530780978 27.639556204487853 22.501267423433287 58-59 22.806106924880527 27.0401287992801 27.71418584074804 22.439578435091335 60-61 22.529446698359973 27.125012021921087 28.11063703890272 22.23490424081622 62-63 22.328291147879785 27.18344413317891 28.07048263218081 22.417782086760496 64-65 22.184557414428827 27.230146567136693 27.884586978427766 22.70070904000671 66-67 22.28931458829841 27.285510722846567 27.596202862473447 22.828971826381576 68-69 22.21554692367093 27.13132926303051 27.8687580717262 22.78436574157236 70-71 22.414391684995902 27.101134256264288 27.73940138870919 22.74507267003062 72-73 22.376849096476473 26.96526070643033 27.82386682192608 22.83402337516712 74-75 22.185578125673867 27.524518048906714 27.38933454090654 22.900569284512873 76-77 22.313983655467634 27.083387382712846 27.538158861936264 23.064470099883252 78-79 22.25656381296745 27.233624214968916 27.561049347521756 22.948762624541878 80-81 22.36559306730875 27.339316438920076 27.524528339318376 22.770562154452794 82-83 22.409022296976755 27.166321645749775 27.558394790184153 22.866261267089317 84-85 22.24621555181783 27.227110018544877 27.448613447190233 23.07806098244706 86-87 22.2170292960191 27.180946254293158 27.529428780516234 23.072595669171502 88-89 22.12374650349991 27.134204347770563 27.349811481745473 23.392237666984055 90-91 22.150727324114587 27.011426521440434 27.51771736848126 23.320128785963725 92-93 22.28142924914823 27.018027342907665 27.50588691939449 23.19465648854962 94-95 22.118191227843187 26.868245137361452 27.574589209470822 23.438974425324535 96-97 21.975697589166344 26.812545823090524 27.71175658774313 23.5 98-99 21.92906661134823 26.99665523279998 27.24282802859279 23.831450127259004 100-101 22.022607593080576 26.32143581073796 27.943688030741498 23.712268565439967 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 368.0 1 250.5 2 196.0 3 258.0 4 233.0 5 248.0 6 422.0 7 930.5 8 1549.5 9 1862.0 10 1785.5 11 1638.5 12 1745.0 13 1945.5 14 2167.5 15 2363.5 16 2471.0 17 2590.0 18 2776.5 19 2989.5 20 3338.5 21 3852.5 22 4599.5 23 5636.0 24 6808.0 25 8468.5 26 10843.0 27 13845.5 28 16833.0 29 20323.0 30 24711.0 31 29407.0 32 34365.0 33 38731.0 34 43912.0 35 48807.0 36 53773.5 37 59216.0 38 63565.0 39 66644.5 40 69027.0 41 73600.5 42 77969.0 43 81147.0 44 84714.0 45 88164.0 46 89542.5 47 94166.0 48 106575.5 49 106410.5 50 95179.5 51 88969.0 52 84175.5 53 81213.0 54 77874.0 55 70726.5 56 62500.5 57 55246.0 58 48908.0 59 42343.5 60 34960.0 61 28174.5 62 22610.0 63 17564.0 64 13058.5 65 9701.0 66 7163.0 67 5651.5 68 4207.5 69 2830.0 70 2040.5 71 1529.0 72 1213.5 73 975.0 74 741.5 75 581.5 76 392.0 77 206.5 78 124.5 79 86.5 80 60.5 81 37.5 82 25.0 83 13.0 84 5.0 85 3.0 86 1.0 87 0.5 88 0.0 89 1.5 90 2.5 91 1.0 92 0.5 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2893431664294648 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0013585198602665287 16-17 7.76297063009445E-4 18-19 1.0781903652908957E-4 20-21 2.1563807305817916E-5 22-23 0.0 24-25 0.0 26-27 8.625522922327166E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0014447750894898005 36-37 0.007784534437400267 38-39 0.002738603527838875 40-41 0.003687411049294863 42-43 4.312761461163583E-5 44-45 0.0014447750894898005 46-47 1.0781903652908957E-4 48-49 2.803294949756329E-4 50-51 0.0 52-53 4.312761461163583E-5 54-55 0.0 56-57 8.409884849268987E-4 58-59 0.002307327381722517 60-61 8.194246776210808E-4 62-63 0.0012722646310432569 64-65 6.469142191745374E-5 66-67 2.5876568766981497E-4 68-69 0.001531030318713072 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.25876568766981495 78-79 8.625522922327166E-5 80-81 1.9407426575236125E-4 82-83 0.0 84-85 0.0 86-87 0.10846595074826412 88-89 0.3757062146892655 90-91 0.279962910251434 92-93 0.0 94-95 0.0 96-97 0.0 98-99 1.509466511407254E-4 100-101 4.312761461163583E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2318700.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.572692268148984 #Duplication Level Percentage of deduplicated Percentage of total 1 75.51674064150643 41.96668588764056 2 13.898693657546653 15.447756511202284 3 4.643491880422004 7.741540359610213 4 2.029425925241315 4.5112264969775655 5 1.0729797777062737 2.9814187498208815 6 0.6294144904478549 2.0986954672063463 7 0.413807108416514 1.6097462566082448 8 0.3080747629908009 1.3696435199416568 9 0.21927899686339983 1.0967331792202313 >10 1.1402649357454782 11.498301834234843 >50 0.08486350514082124 3.2459051969264436 >100 0.040087565936250506 4.0785584920215365 >500 0.0017105012107067085 0.6287166806489363 >1k 9.330006603850251E-4 0.9402017023514295 >5k 2.3325016509625628E-4 0.7848696655888656 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6495 0.2801138569025747 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6176 0.2663561478414629 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5494 0.23694311467632725 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3579 0.15435373269504463 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3203 0.13813774960106956 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 2.1563807305817916E-5 14-15 0.0 0.0 0.0 0.0 4.312761461163583E-5 16-17 0.0 0.0 0.0 0.0 4.312761461163583E-5 18-19 4.312761461163583E-5 0.0 0.0 0.0 4.312761461163583E-5 20-21 4.312761461163583E-5 0.0 0.0 4.312761461163583E-5 4.312761461163583E-5 22-23 4.312761461163583E-5 0.0 0.0 4.312761461163583E-5 4.312761461163583E-5 24-25 8.625522922327166E-5 0.0 0.0 1.509466511407254E-4 4.312761461163583E-5 26-27 8.625522922327166E-5 0.0 0.0 3.8814853150472245E-4 4.312761461163583E-5 28-29 8.625522922327166E-5 0.0 0.0 0.0016819769698537975 4.312761461163583E-5 30-31 8.625522922327166E-5 0.0 0.0 0.005757536550653383 4.312761461163583E-5 32-33 8.625522922327166E-5 0.0 0.0 0.014986846077543452 4.312761461163583E-5 34-35 8.625522922327166E-5 0.0 0.0 0.02887393798249019 4.312761461163583E-5 36-37 8.625522922327166E-5 0.0 0.0 0.05162375469012809 4.312761461163583E-5 38-39 8.625522922327166E-5 8.625522922327166E-5 0.0 0.08569457023332039 4.312761461163583E-5 40-41 8.625522922327166E-5 8.625522922327166E-5 0.0 0.12340966921119592 4.312761461163583E-5 42-43 1.0781903652908957E-4 8.625522922327166E-5 0.0 0.16485530685297797 4.312761461163583E-5 44-45 1.2938284383490748E-4 8.625522922327166E-5 0.0 0.21619873204813042 4.312761461163583E-5 46-47 1.2938284383490748E-4 8.625522922327166E-5 0.0 0.28248587570621464 4.312761461163583E-5 48-49 1.2938284383490748E-4 8.625522922327166E-5 0.0 0.3507137620218226 4.312761461163583E-5 50-51 1.2938284383490748E-4 8.625522922327166E-5 0.0 0.43153491180402814 4.312761461163583E-5 52-53 1.2938284383490748E-4 8.625522922327166E-5 0.0 0.5091861819122785 4.312761461163583E-5 54-55 1.2938284383490748E-4 8.625522922327166E-5 0.0 0.6001207573209126 4.312761461163583E-5 56-57 1.2938284383490748E-4 8.625522922327166E-5 0.0 0.7102471212317247 4.312761461163583E-5 58-59 1.2938284383490748E-4 8.625522922327166E-5 0.0 0.8363953939707595 4.312761461163583E-5 60-61 1.2938284383490748E-4 8.625522922327166E-5 0.0 0.974662526415664 4.312761461163583E-5 62-63 1.2938284383490748E-4 8.625522922327166E-5 0.0 1.1180402811920473 4.312761461163583E-5 64-65 1.2938284383490748E-4 8.625522922327166E-5 0.0 1.2696338465519472 4.312761461163583E-5 66-67 1.2938284383490748E-4 8.625522922327166E-5 0.0 1.445055418984776 4.312761461163583E-5 68-69 1.2938284383490748E-4 8.625522922327166E-5 0.0 1.6492431103635656 4.312761461163583E-5 70-71 1.2938284383490748E-4 8.625522922327166E-5 0.0 1.8787467115193859 4.312761461163583E-5 72-73 1.2938284383490748E-4 8.625522922327166E-5 0.0 2.113662828308966 4.312761461163583E-5 74-75 1.2938284383490748E-4 8.625522922327166E-5 0.0 2.3560615862336656 4.312761461163583E-5 76-77 1.2938284383490748E-4 8.625522922327166E-5 0.0 2.6235390520550306 4.312761461163583E-5 78-79 1.2938284383490748E-4 8.625522922327166E-5 0.0 2.939966360460603 4.312761461163583E-5 80-81 1.7251045844654333E-4 8.625522922327166E-5 0.0 3.280588260663303 4.312761461163583E-5 82-83 1.7251045844654333E-4 8.625522922327166E-5 0.0 3.634083753827576 4.312761461163583E-5 84-85 1.9407426575236122E-4 8.625522922327166E-5 0.0 3.9968732479406563 4.312761461163583E-5 86-87 2.1563807305817915E-4 8.625522922327166E-5 0.0 4.382736015870963 4.312761461163583E-5 88-89 2.1563807305817915E-4 8.625522922327166E-5 0.0 4.80648639323759 4.312761461163583E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9515 0.0 23.710625 1 TACATGG 9715 0.0 22.663456 2 ACATGGG 9700 0.0 22.20931 3 GAGTACT 6980 0.0 21.212292 12-13 GTATCAA 15985 0.0 20.009216 1 CATGGGG 4935 0.0 19.615255 4 GTACTTT 7680 0.0 19.186193 14-15 AGTACTT 7425 0.0 18.950325 12-13 AGAGTAC 12990 0.0 18.704231 10-11 GGTATCA 12875 0.0 18.262201 1 GTAAGCG 190 2.1100277E-10 17.482172 94-95 ATCAACG 18310 0.0 17.208012 3 TCAACGC 18390 0.0 17.18476 4 CAACGCA 18450 0.0 17.128874 5 TATCAAC 18695 0.0 16.955162 2 AACGCAG 18765 0.0 16.816051 6 GAGTACA 8240 0.0 16.693373 1 CATGGGA 5430 0.0 16.516306 4 ACTTTTT 9015 0.0 16.29181 16-17 TACTTTT 8680 0.0 16.237724 14-15 >>END_MODULE