FastQCFastQC Report
Fri 27 May 2016
SRR939286_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939286_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2351174
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65940.2804556362055722No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT58030.24681286880511608No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51800.22031546793219048No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT34240.14562937494205022No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32940.14010022227193736No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG99650.024.2622131
TACATGG101150.023.0222852
ACATGGG100100.022.505693
GTATCAA152400.021.1940441
GAGTACT66500.021.06339312-13
AGTACTT69800.019.62613912-13
GTACTTT71100.019.53417614-15
CATGGGG51350.019.4883384
AGAGTAC127850.018.5748310-11
GGTATCA118950.018.448771
CATGGGA52250.018.1541734
TCAACGC175050.018.1257844
CAACGCA175550.018.1011775
ATCAACG176150.018.039523
TATCAAC177450.017.9608172
AGTACAT77700.017.8846072
AACGCAG180050.017.6912846
GAGTACA87900.017.5624221
TACTTTT81600.016.78822514-15
ACTTTTT86350.016.30406216-17
Produced by FastQC (version 0.11.5)