FastQCFastQC Report
Fri 27 May 2016
SRR939286_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939286_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2351174
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65570.2788819542917708No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT62260.2648038809547911No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55190.23473379681810025No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37470.15936719273009994No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT30630.1302753432965829No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG98900.022.7546081
TACATGG100050.021.8646032
GAGTACT71250.021.5792512-13
GTATCAA161100.021.0275651
GTACTTT73950.020.66302714-15
ACATGGG100150.020.6108573
GGTATCA126300.019.626321
AGTACTT74500.019.26838512-13
AGAGTAC132750.018.87450810-11
ATCAACG182750.018.4616263
TCAACGC184150.018.2697354
CAACGCA185200.018.1661555
TATCAAC186450.018.1207162
AACGCAG189800.017.8008826
GTGTAGG10400.017.3842011
CATGGGG48850.017.1935864
ACTTTTT90300.017.05236816-17
GGACCGA2858.682364E-716.6499486
GTGTAGC12450.016.4324991
TACCTGG20300.016.362882
Produced by FastQC (version 0.11.5)