##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939286_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2351174 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.247056576842034 33.0 31.0 34.0 31.0 34.0 2 32.44833389617272 34.0 31.0 34.0 31.0 34.0 3 32.54090892464786 34.0 31.0 34.0 31.0 34.0 4 36.0817706388383 37.0 35.0 37.0 35.0 37.0 5 36.01768775939169 37.0 35.0 37.0 35.0 37.0 6 36.0535728108596 37.0 35.0 37.0 35.0 37.0 7 35.99790955497126 37.0 35.0 37.0 35.0 37.0 8 35.97351025487693 37.0 35.0 37.0 35.0 37.0 9 37.67296508042365 39.0 37.0 39.0 35.0 39.0 10-11 37.589390023877435 39.0 37.0 39.0 35.0 39.0 12-13 37.510076242762125 39.0 37.0 39.0 35.0 39.0 14-15 38.796211594718216 40.0 38.0 41.0 35.0 41.0 16-17 38.74580677567887 40.0 38.0 41.0 34.0 41.0 18-19 38.856566336647134 40.0 38.0 41.0 35.0 41.0 20-21 38.861752894511426 40.0 38.0 41.0 34.5 41.0 22-23 38.80911195853646 40.0 38.0 41.0 34.0 41.0 24-25 38.74438854801899 40.0 38.0 41.0 34.0 41.0 26-27 38.583578884421144 40.0 38.0 41.0 34.0 41.0 28-29 38.43930011134863 40.0 38.0 41.0 34.0 41.0 30-31 38.26832361194875 40.0 38.0 41.0 34.0 41.0 32-33 38.081577756474005 40.0 37.5 41.0 33.0 41.0 34-35 38.04161325363414 40.0 37.5 41.0 33.0 41.0 36-37 37.97179855680609 40.0 37.5 41.0 33.0 41.0 38-39 37.879557404088345 40.0 37.0 41.0 33.0 41.0 40-41 37.72832104301936 40.0 37.0 41.0 33.0 41.0 42-43 37.56332623616967 40.0 37.0 41.0 32.5 41.0 44-45 37.358074306708055 40.0 37.0 41.0 32.0 41.0 46-47 37.08845942495111 40.0 37.0 41.0 31.0 41.0 48-49 36.79936555099708 40.0 36.0 41.0 30.5 41.0 50-51 36.574469605397134 39.0 36.0 41.0 30.0 41.0 52-53 36.358266763752916 39.0 35.5 41.0 30.0 41.0 54-55 36.09261437052298 39.0 35.0 40.5 29.5 41.0 56-57 35.85101719396353 39.0 35.0 40.0 29.0 41.0 58-59 35.55739494397267 38.0 35.0 40.0 28.0 41.0 60-61 35.22347899389837 38.0 34.0 40.0 28.0 41.0 62-63 34.855793743891354 37.5 34.0 40.0 27.0 41.0 64-65 34.43771515846977 37.0 34.0 40.0 26.0 41.0 66-67 34.059617025366904 36.5 33.5 39.0 26.0 40.5 68-69 33.67200747371313 36.0 33.0 39.0 26.0 40.0 70-71 33.27055951622466 35.5 33.0 38.5 25.5 40.0 72-73 32.76699108615526 35.0 32.0 38.0 24.5 40.0 74-75 32.25598403180709 35.0 32.0 37.0 22.5 39.0 76-77 30.25979765853144 33.5 29.5 35.5 20.0 37.5 78-79 31.244162916058105 34.0 31.0 36.0 20.5 38.0 80-81 31.27953481962628 34.5 31.0 36.0 21.0 37.5 82-83 30.95819003612663 34.0 31.0 35.0 20.0 37.0 84-85 30.575893787529125 34.0 31.0 35.0 18.5 36.5 86-87 30.0403589866169 34.0 30.0 35.0 14.5 36.0 88-89 29.52879093593243 34.0 30.0 35.0 7.0 36.0 90-91 29.10723472614107 34.0 29.0 35.0 4.0 35.0 92-93 28.748129657779476 34.0 29.0 35.0 2.0 35.0 94-95 28.34035698761555 34.0 29.0 35.0 2.0 35.0 96-97 27.773505916618678 33.0 28.5 35.0 2.0 35.0 98-99 27.00959924701447 33.0 27.0 35.0 2.0 35.0 100-101 23.635978876935525 29.5 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.16321041115424606 1101 2 0.21577889755276658 1101 3 0.2252872208596628 1101 4 0.16593913850221753 1101 5 0.2088512237855369 1101 6 0.20788930196250988 1101 7 0.15779532257994333 1101 8 0.23829215181864782 1101 9 0.3201705714008085 1101 10-11 0.29791856280803586 1101 12-13 0.3328588887515451 1101 14-15 0.4299809792919831 1101 16-17 0.41013226630135335 1101 18-19 0.26754062670224954 1101 20-21 0.23593927802461678 1101 22-23 0.3164605074779985 1101 24-25 0.2593424462155909 1101 26-27 0.30185006225335087 1101 28-29 0.31795160910984777 1101 30-31 0.3986533127253651 1101 32-33 0.3250106282094123 1101 34-35 0.38916984803405086 1101 36-37 0.429333335711533 1101 38-39 0.4490145010271078 1101 40-41 0.502784560899677 1101 42-43 0.5925046738846547 1101 44-45 0.5856527742712103 1101 46-47 0.5853292136407049 1101 48-49 0.5499952764188194 1101 50-51 0.6414970564081202 1101 52-53 0.6022076068960303 1101 54-55 0.6041203363533825 1101 56-57 0.551393100455968 1101 58-59 0.3847244571718704 1101 60-61 0.5071920611431793 1101 62-63 0.3787177927615133 1101 64-65 0.47484264223019323 1101 66-67 0.4682718564502082 1101 68-69 0.49674275646616906 1101 70-71 0.5371975493593126 1101 72-73 0.5211895236492339 1101 74-75 0.4317979724633503 1101 76-77 0.5973539847588434 1101 78-79 0.719621718967538 1101 80-81 0.759500253530728 1101 82-83 0.7033176925711473 1101 84-85 0.6286152912256675 1101 86-87 0.7018514507083502 1101 88-89 0.7400589136842619 1101 90-91 0.8474346694492354 1101 92-93 0.7057680254058276 1101 94-95 0.7617079485500966 1101 96-97 0.7401629933494895 1101 98-99 0.5995460038817519 1101 100-101 0.6450237873269771 1102 1 0.20641760729840541 1102 2 0.1884808258420918 1102 3 0.21799252337871877 1102 4 0.09298524928757956 1102 5 0.18050769400934286 1102 6 0.11799281521788885 1102 7 0.16982408281786832 1102 8 0.17662625956536715 1102 9 0.24784681497962424 1102 10-11 0.19805196473226516 1102 12-13 0.20814330128524006 1102 14-15 0.28141576446602556 1102 16-17 0.30784286064518795 1102 18-19 0.30654063776530194 1102 20-21 0.33748077551882716 1102 22-23 0.24916905515789978 1102 24-25 0.2477598955929281 1102 26-27 0.33291210567212204 1102 28-29 0.2736565465483096 1102 30-31 0.3357140175152935 1102 32-33 0.22824352750248522 1102 34-35 0.3761000088538964 1102 36-37 0.4886633114097094 1102 38-39 0.4055669140623337 1102 40-41 0.5312654568590816 1102 42-43 0.43146587208654097 1102 44-45 0.4868466154722171 1102 46-47 0.44846063712313367 1102 48-49 0.30915017937745404 1102 50-51 0.3975501571961786 1102 52-53 0.42888559474880594 1102 54-55 0.4639220127739705 1102 56-57 0.4221193066048059 1102 58-59 0.28408459811510056 1102 60-61 0.2990191420103727 1102 62-63 0.3234288259395939 1102 64-65 0.20044331965751638 1102 66-67 0.3251517357154725 1102 68-69 0.4424785044941757 1102 70-71 0.32452773156771286 1102 72-73 0.4729735323816655 1102 74-75 0.5073023552743265 1102 76-77 0.65341920880401 1102 78-79 0.6226861372540178 1102 80-81 0.6486130154945009 1102 82-83 0.4547448258034379 1102 84-85 0.5485416389815256 1102 86-87 0.5631687647477293 1102 88-89 0.7169479752796271 1102 90-91 0.8994713305474988 1102 92-93 0.8350362877328301 1102 94-95 0.9598462219032733 1102 96-97 0.8113260516185683 1102 98-99 0.7442518282079185 1102 100-101 0.7261698638168923 1103 1 0.15762932821512265 1103 2 0.19405842828658137 1103 3 0.225907341186236 1103 4 0.17143846775034177 1103 5 0.15088228275595839 1103 6 0.18156461346265473 1103 7 0.12505250482750085 1103 8 0.10864891377791253 1103 9 0.12906295753091968 1103 10-11 0.23584567939251855 1103 12-13 0.28174053541952304 1103 14-15 0.40470375995797525 1103 16-17 0.30371079257959366 1103 18-19 0.28923972852647495 1103 20-21 0.37126973944987895 1103 22-23 0.34368402206917636 1103 24-25 0.297501445996339 1103 26-27 0.3468728882554757 1103 28-29 0.36284785624382465 1103 30-31 0.39351792675504527 1103 32-33 0.3427553597414459 1103 34-35 0.3935838505832834 1103 36-37 0.5721809561217199 1103 38-39 0.5134148098331011 1103 40-41 0.515730443672318 1103 42-43 0.5628369097366246 1103 44-45 0.6521415182743908 1103 46-47 0.57413801591197 1103 48-49 0.5765728904001932 1103 50-51 0.5452736588733629 1103 52-53 0.3563064396762883 1103 54-55 0.495658271924043 1103 56-57 0.6040180907012669 1103 58-59 0.5515054706203557 1103 60-61 0.5290337674170331 1103 62-63 0.41061799008519273 1103 64-65 0.6118722798012115 1103 66-67 0.5525057964950193 1103 68-69 0.548108228909598 1103 70-71 0.5729840663176304 1103 72-73 0.7090934147533119 1103 74-75 0.6871054592434049 1103 76-77 0.6404330703066492 1103 78-79 0.8567094202395538 1103 80-81 0.7753330635165732 1103 82-83 0.7695511885447779 1103 84-85 0.6193833950231245 1103 86-87 0.625151624249142 1103 88-89 0.9524491965148698 1103 90-91 1.0024961054899784 1103 92-93 0.6880964541370496 1103 94-95 0.8213240406493476 1103 96-97 0.649124684313108 1103 98-99 0.6393807220730601 1103 100-101 0.5953588845581308 1104 1 0.11850290040899836 1104 2 0.11055197251452142 1104 3 0.13958386251479737 1104 4 0.1368923693611066 1104 5 0.1618343956366317 1104 6 0.08023759365042338 1104 7 0.06094786865701707 1104 8 0.14150479842752617 1104 9 0.18905124452675892 1104 10-11 0.20268289587425414 1104 12-13 0.19050440488762632 1104 14-15 0.2277599914577877 1104 16-17 0.2508631251925948 1104 18-19 0.24594905534653577 1104 20-21 0.18817210384234784 1104 22-23 0.16932074642314632 1104 24-25 0.17531188600578673 1104 26-27 0.2063120714323361 1104 28-29 0.21269463380570386 1104 30-31 0.24069545594675645 1104 32-33 0.37052403244923937 1104 34-35 0.29689549821253536 1104 36-37 0.39239882707892093 1104 38-39 0.4919818281925501 1104 40-41 0.4670356718488975 1104 42-43 0.504204244074792 1104 44-45 0.5157513027187974 1104 46-47 0.5138469159791654 1104 48-49 0.3263284154878079 1104 50-51 0.3331867919657796 1104 52-53 0.43788637865971225 1104 54-55 0.35455743113009675 1104 56-57 0.3982505711341915 1104 58-59 0.37508651734525245 1104 60-61 0.4032281578868293 1104 62-63 0.5279200947940055 1104 64-65 0.4793146681644771 1104 66-67 0.5033213938582364 1104 68-69 0.34480861207919133 1104 70-71 0.4062612559230203 1104 72-73 0.407139658420121 1104 74-75 0.43874686879903635 1104 76-77 0.5061496065261615 1104 78-79 0.6594547123382668 1104 80-81 0.644502439004615 1104 82-83 0.6252826157153173 1104 84-85 0.4092893277959284 1104 86-87 0.5739939436055579 1104 88-89 0.8125947918805174 1104 90-91 0.9291522697989087 1104 92-93 0.8938471759849804 1104 94-95 0.9150371537660931 1104 96-97 0.8887852341887132 1104 98-99 0.7251178695479545 1104 100-101 0.6758590025961553 1105 1 0.09723449946988438 1105 2 0.13058607530273036 1105 3 0.13033712284416765 1105 4 0.0914302680124166 1105 5 0.12637092332158772 1105 6 0.21475038648026867 1105 7 0.165731684399141 1105 8 0.17593790383769914 1105 9 0.17819276252926386 1105 10-11 0.13061847759241374 1105 12-13 0.18427336278183049 1105 14-15 0.2385347159792346 1105 16-17 0.2412935657869113 1105 18-19 0.21022874105249656 1105 20-21 0.1769149705330051 1105 22-23 0.1581317330048151 1105 24-25 0.22053471088197085 1105 26-27 0.2523848954516694 1105 28-29 0.19467520892609258 1105 30-31 0.277000787292188 1105 32-33 0.15248275779326548 1105 34-35 0.26075618232474085 1105 36-37 0.2861949900868126 1105 38-39 0.22301536545712963 1105 40-41 0.2297903716929497 1105 42-43 0.1436362355748031 1105 44-45 0.28276670895326816 1105 46-47 0.2848304129716084 1105 48-49 0.36301954533546166 1105 50-51 0.2979617392692617 1105 52-53 0.22544850057289523 1105 54-55 0.06961064176194753 1105 56-57 0.1486618610421644 1105 58-59 0.11453243272235625 1105 60-61 0.044227977947493 1105 62-63 0.10007105532731231 1105 64-65 0.19185643979575673 1105 66-67 0.1449261528252137 1105 68-69 0.16097744658641489 1105 70-71 0.31545744120911934 1105 72-73 0.0928938184461714 1105 74-75 0.20365420004164747 1105 76-77 0.46371669819240324 1105 78-79 0.31482847204213726 1105 80-81 0.37962402658365946 1105 82-83 0.293638355780093 1105 84-85 0.25532552130782804 1105 86-87 0.2628042875467429 1105 88-89 0.5204185113122541 1105 90-91 0.536527640388254 1105 92-93 0.5505456319341171 1105 94-95 0.5338613787416726 1105 96-97 0.35633853050595476 1105 98-99 0.6597148482808386 1105 100-101 0.5384179113069365 1106 1 0.1282408646281965 1106 2 0.11703187004290072 1106 3 0.0733740063627053 1106 4 0.11021561845310401 1106 5 0.013843800104602622 1106 6 0.07729890808493423 1106 7 0.10646220709988796 1106 8 0.07217823754707808 1106 9 0.14983330693669927 1106 10-11 0.15087342061997333 1106 12-13 0.161845215728043 1106 14-15 0.2008718296267844 1106 16-17 0.13441257449189692 1106 18-19 0.05815790447897484 1106 20-21 0.2259786338801817 1106 22-23 0.1494787726646436 1106 24-25 0.23490185204520486 1106 26-27 0.29984702639033145 1106 28-29 0.2279683619693884 1106 30-31 0.23335537825034436 1106 32-33 0.19343278130106256 1106 34-35 0.23856493874781393 1106 36-37 0.2553224916191823 1106 38-39 0.39851439411231127 1106 40-41 0.33708989922466515 1106 42-43 0.27763304062312244 1106 44-45 0.383131029180241 1106 46-47 0.4618636350333247 1106 48-49 0.3187628117395036 1106 50-51 0.3233103098772432 1106 52-53 0.31233873272613266 1106 54-55 0.3448411662881483 1106 56-57 0.37045219416334163 1106 58-59 0.39610215353728506 1106 60-61 0.2910717664721929 1106 62-63 0.4778094911721027 1106 64-65 0.47836705846675187 1106 66-67 0.5286311893363305 1106 68-69 0.43392858646129184 1106 70-71 0.3418240071512457 1106 72-73 0.3786434252634763 1106 74-75 0.42088059173801895 1106 76-77 0.38360995776113427 1106 78-79 0.555411254595171 1106 80-81 0.661159976483443 1106 82-83 0.5244242262725898 1106 84-85 0.47883056503964383 1106 86-87 0.46786797766726096 1106 88-89 0.8035136025018339 1106 90-91 0.7253451877963322 1106 92-93 0.7003344470881494 1106 94-95 0.7279379602245051 1106 96-97 0.8503192958907881 1106 98-99 0.7859895735600197 1106 100-101 0.5307605337716268 1107 1 0.10920160323918537 1107 2 0.10297859725246639 1107 3 0.1088923661835608 1107 4 0.03885614032546414 1107 5 0.07802117498673766 1107 6 0.1029249626231703 1107 7 0.11387305209517251 1107 8 0.15796182148832116 1107 9 0.20615030113053479 1107 10-11 0.18737973156607524 1107 12-13 0.20590911531740375 1107 14-15 0.24811998883723874 1107 16-17 0.11424346455317647 1107 18-19 0.2014996973800791 1107 20-21 0.20499845997441923 1107 22-23 0.2405307831107777 1107 24-25 0.2428711758380686 1107 26-27 0.3096860232143115 1107 28-29 0.20599422762121833 1107 30-31 0.3138913744477989 1107 32-33 0.23101931546810306 1107 34-35 0.31393641147248985 1107 36-37 0.42305920443740774 1107 38-39 0.2981270063896204 1107 40-41 0.334899600045965 1107 42-43 0.2841488850601124 1107 44-45 0.3642231973728798 1107 46-47 0.3077516919225616 1107 48-49 0.45674884918801695 1107 50-51 0.321769404649217 1107 52-53 0.40262940145845505 1107 54-55 0.35042188343618363 1107 56-57 0.4507924998553676 1107 58-59 0.36486475371841465 1107 60-61 0.3414512645430108 1107 62-63 0.32959724364096843 1107 64-65 0.43450989877244695 1107 66-67 0.4262323280825555 1107 68-69 0.5185229071525725 1107 70-71 0.4501414642563475 1107 72-73 0.44861387880796144 1107 74-75 0.4685252689562702 1107 76-77 0.6058359600376946 1107 78-79 0.8083393428414141 1107 80-81 0.6767408758494788 1107 82-83 0.7372372147719197 1107 84-85 0.674357461967837 1107 86-87 0.7418775258906756 1107 88-89 0.8629977006855469 1107 90-91 0.7890939626710285 1107 92-93 0.7109478704294219 1107 94-95 0.6867426372566605 1107 96-97 0.7964994637484644 1107 98-99 0.938388473583597 1107 100-101 0.9159836422712075 1108 1 -0.024946409704760697 1108 2 0.033222143108979196 1108 3 0.015579736922397558 1108 4 -0.012686190476131287 1108 5 0.03449972209129015 1108 6 0.05247522292840756 1108 7 0.0726799715788431 1108 8 0.006895639773873086 1108 9 0.00892897036459317 1108 10-11 0.09879042770429436 1108 12-13 0.039652239960815905 1108 14-15 0.021593704248736856 1108 16-17 0.04213105860181798 1108 18-19 -0.025638349082633738 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12.0 5 50.0 6 80.0 7 229.0 8 613.0 9 1070.0 10 1794.0 11 2837.0 12 4181.0 13 5950.0 14 7289.0 15 9664.0 16 12770.0 17 17968.0 18 17269.0 19 12039.0 20 11386.0 21 12245.0 22 13144.0 23 14901.0 24 16788.0 25 20452.0 26 24709.0 27 30605.0 28 37018.0 29 45156.0 30 55232.0 31 67211.0 32 83309.0 33 106830.0 34 143328.0 35 203612.0 36 319943.0 37 533140.0 38 474937.0 39 43401.0 40 2.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.444628114681876 19.590691160201672 15.245766983881657 22.718913741234793 2 15.406048212510006 20.79280393539568 40.03008709691414 23.77106075518018 3 19.578474413208042 24.57329827566994 29.994972724264557 25.85325458685746 4 13.17805487811621 17.120170604132234 37.01563559311221 32.68613892463935 5 13.84197851796592 37.65378487513047 34.02355589165242 14.48068071525119 6 31.82099665954115 36.19949012705993 17.45379116985812 14.525722043540801 7 28.60251942221205 32.73152050847789 20.577847492359137 18.08811257695092 8 26.032994580579743 35.68191890519374 19.48460641364697 18.800480100579538 9 26.041118181810447 17.60724642242556 20.443616678306242 35.90801871745774 10-11 25.029474636926064 26.87914633285329 27.015397414227955 21.075981615992692 12-13 25.973875178953154 24.030909664703675 27.74318276741747 22.252032388925702 14-15 22.651842995494246 25.581785071654622 26.305147219104892 25.46122471374624 16-17 22.099077177746956 28.92356826872897 26.796764316462063 22.18059023706201 18-19 22.01504439909594 27.597425796644572 28.983882094647186 21.403647709612304 20-21 22.942623557422802 26.497060617376683 28.807119336978037 21.75319648822248 22-23 22.495782957790446 26.478495423988186 28.807353262667927 22.218368355553437 24-25 22.55731772748935 26.6284601648413 28.892098637678554 21.922123469990797 26-27 22.273121858706048 26.800179147060938 28.981992147642355 21.944706846590655 28-29 21.93923120959997 27.196200706540647 29.00893340943716 21.855634674422227 30-31 22.19633680876022 26.692941483701333 28.86304884283341 22.247672864705038 32-33 22.144532901435625 26.798335639982408 28.795720776088878 22.261410682493086 34-35 22.71343376140811 26.80659308595367 28.53017074360642 21.949802409031808 36-37 22.575254677706035 26.55654810777421 28.77213566815195 22.096061546367807 38-39 22.23175958363037 26.929720611079254 28.85616600339942 21.982353801890948 40-41 22.337262625918868 26.813189150558124 28.75810390008168 22.09144432344133 42-43 22.196935379249478 26.84979955551302 28.386405929328696 22.566859135908807 44-45 22.43442012334765 26.823573645258858 28.286043545748385 22.455962685645105 46-47 22.60680852511728 27.244627143081956 27.901725523888103 22.246838807912656 48-49 22.60711032020599 27.14182361662727 27.928898499217837 22.322167563948902 50-51 22.28737643407081 27.26759057390053 28.083417050375687 22.36161594165298 52-53 22.66346996510466 27.403443429419394 27.59617697290311 22.33690963257284 54-55 22.299583101888672 27.136613453534274 27.7767617369025 22.78704170767455 56-57 22.495298624282725 27.37541487261574 27.663101000415324 22.466185502686212 58-59 22.766282501523715 27.022098462928284 27.747740582410636 22.46387845313737 60-61 22.54682790121598 27.124646283945154 28.15564910006299 22.172876714775878 62-63 22.32229935294971 27.213753721081467 28.06351496364941 22.400431962319413 64-65 22.208697061276 27.25572995782744 27.85443098946122 22.681141991435343 66-67 22.27227759408704 27.30072295797759 27.618457842762805 22.808541605172564 68-69 22.22110457012903 27.14986523667689 27.831167522060397 22.797862671133686 70-71 22.40516865191602 27.1199196656649 27.743161501445663 22.73175018097342 72-73 22.35999972779556 26.96087146251192 27.871714301025786 22.807414508666735 74-75 22.17409260224892 27.505365404687186 27.432104131808195 22.888437861255696 76-77 22.28406770282595 27.10577005261081 27.571984182012997 23.038178062550234 78-79 22.26557881296748 27.194648290598657 27.596936679292984 22.942836217140883 80-81 22.38293581087691 27.357658775762967 27.499632094306353 22.75977331905377 82-83 22.3917710896769 27.16821468764115 27.63149388348119 22.808520339200758 84-85 22.27906143909383 27.2026230300267 27.475337852494114 23.042977678385352 86-87 22.208713630582903 27.194065170214643 27.572868692657376 23.02435250654508 88-89 22.13265790885611 27.135361911676643 27.398399281550507 23.333580897916743 90-91 22.148709249743675 27.07282394250166 27.534354983425434 23.244111824329227 92-93 22.294819524203653 27.01433411563755 27.534691179810594 23.1561551803482 94-95 22.095455737315856 26.937484622040863 27.585692846572147 23.38136679407113 96-97 21.99056726554479 26.895117077681192 27.71876943178174 23.39554622499228 98-99 21.952033324628463 27.037918078372762 27.27890407090245 23.731144526096323 100-101 21.93795731191876 26.492133814993927 27.88777849680468 23.682130376282633 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 326.0 1 234.5 2 196.5 3 273.0 4 236.5 5 225.5 6 406.5 7 1005.0 8 1666.0 9 1916.5 10 1790.0 11 1670.5 12 1805.5 13 2001.0 14 2238.5 15 2372.5 16 2453.0 17 2563.5 18 2820.5 19 3070.0 20 3374.0 21 3950.5 22 4728.5 23 5822.0 24 7035.5 25 8562.5 26 10914.5 27 14173.0 28 17216.5 29 20771.5 30 25313.0 31 29752.5 32 34630.0 33 38989.5 34 44169.0 35 49695.0 36 55060.0 37 60379.0 38 64497.5 39 67814.5 40 70276.5 41 74740.0 42 79471.5 43 82711.0 44 85920.0 45 89296.0 46 91106.0 47 96121.5 48 108438.5 49 107852.5 50 96276.5 51 89962.0 52 84755.0 53 81484.0 54 78528.0 55 71660.5 56 63095.5 57 56055.0 58 49759.5 59 42484.5 60 35051.0 61 28521.0 62 22940.0 63 17835.5 64 13345.5 65 9745.0 66 7122.5 67 5655.0 68 4175.0 69 2797.5 70 2086.5 71 1541.5 72 1182.0 73 992.5 74 767.5 75 558.0 76 370.0 77 206.5 78 130.5 79 77.5 80 41.0 81 27.5 82 19.5 83 11.0 84 7.5 85 4.5 86 3.0 87 2.5 88 2.5 89 2.0 90 1.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27186418359508907 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0029559700813295824 16-17 7.230430414763008E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.4886180265688545E-4 26-27 0.0014035541393363484 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.004465854079706563 36-37 0.02364776065063666 38-39 0.007910941512623056 40-41 0.019309502401778856 42-43 0.006337259598821695 44-45 6.167131824356683E-4 46-47 1.7012777446501196E-4 48-49 0.0 50-51 0.0 52-53 2.1265971808126495E-5 54-55 0.0 56-57 2.1265971808126495E-5 58-59 6.379791542437948E-4 60-61 0.002764576335056444 62-63 0.0018288735754988783 64-65 2.1265971808126495E-5 66-67 0.0 68-69 6.379791542437948E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.22812007958577288 78-79 0.0 80-81 0.0011908944212550836 82-83 0.0 84-85 0.0 86-87 0.1047561771268311 88-89 0.3551842611393287 90-91 0.3584592207977802 92-93 0.0 94-95 2.1265971808126495E-5 96-97 0.0 98-99 0.0 100-101 1.0632985904063247E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2351174.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.39138107551685 #Duplication Level Percentage of deduplicated Percentage of total 1 75.38319602332807 41.755793376185515 2 14.059317084823542 15.575299806139709 3 4.587711126939046 7.6235896589000856 4 2.0059536905167716 4.444501811650156 5 1.0814330352083468 2.995103468043918 6 0.6652524513248411 2.2109551225653594 7 0.4159538718021873 1.6128181595982145 8 0.29546915733680634 1.3093155752083907 9 0.22390053003135077 1.1161943623779078 >10 1.1546302621869982 11.6486903124219 >50 0.08275970414036063 3.16683504519251 >100 0.04150111307441784 4.182737051553904 >500 0.0018454416552131584 0.6877381505145554 >1k 8.458274253053153E-4 0.8909035049423231 >5k 2.3068020690144965E-4 0.7795245947054738 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6557 0.2788819542917708 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6226 0.2648038809547911 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5519 0.23473379681810025 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3747 0.15936719273009994 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3063 0.1302753432965829 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 4.253194361625299E-5 0.0 18-19 0.0 0.0 0.0 4.253194361625299E-5 0.0 20-21 0.0 0.0 0.0 4.253194361625299E-5 4.253194361625299E-5 22-23 0.0 0.0 0.0 1.9139374627313843E-4 4.253194361625299E-5 24-25 0.0 0.0 0.0 4.040534643544034E-4 4.253194361625299E-5 26-27 0.0 0.0 0.0 7.655749850925537E-4 4.253194361625299E-5 28-29 2.1265971808126495E-5 0.0 0.0 0.0024668527297426733 4.253194361625299E-5 30-31 4.253194361625299E-5 0.0 0.0 0.007145366527530502 4.253194361625299E-5 32-33 4.253194361625299E-5 0.0 0.0 0.016842649672036185 4.253194361625299E-5 34-35 4.253194361625299E-5 0.0 0.0 0.03117591467071344 4.253194361625299E-5 36-37 4.253194361625299E-5 0.0 0.0 0.054377089913379445 4.253194361625299E-5 38-39 4.253194361625299E-5 0.0 0.0 0.0877433996803299 4.253194361625299E-5 40-41 4.253194361625299E-5 0.0 0.0 0.12442720104934811 4.253194361625299E-5 42-43 8.506388723250598E-5 0.0 0.0 0.16785231548154242 4.253194361625299E-5 44-45 1.7012777446501196E-4 0.0 0.0 0.2196987547497548 4.253194361625299E-5 46-47 1.7012777446501196E-4 0.0 0.0 0.28413464932837806 4.253194361625299E-5 48-49 1.7012777446501196E-4 0.0 0.0 0.35346171742287047 4.253194361625299E-5 50-51 1.7012777446501196E-4 0.0 0.0 0.43018934370659084 4.253194361625299E-5 52-53 1.7012777446501196E-4 0.0 0.0 0.5065341824977649 4.253194361625299E-5 54-55 1.7012777446501196E-4 0.0 0.0 0.5962127856126344 4.253194361625299E-5 56-57 1.7012777446501196E-4 0.0 0.0 0.706774573043084 4.253194361625299E-5 58-59 1.7012777446501196E-4 0.0 0.0 0.8324989983727278 4.253194361625299E-5 60-61 1.7012777446501196E-4 0.0 0.0 0.9685374200293131 4.253194361625299E-5 62-63 1.7012777446501196E-4 0.0 0.0 1.1086163763294423 4.253194361625299E-5 64-65 1.7012777446501196E-4 0.0 0.0 1.2608594685038197 4.253194361625299E-5 66-67 1.7012777446501196E-4 0.0 0.0 1.4386642587915652 4.253194361625299E-5 68-69 1.7012777446501196E-4 0.0 0.0 1.6433067055011668 4.253194361625299E-5 70-71 1.7012777446501196E-4 0.0 0.0 1.8663654837966055 4.253194361625299E-5 72-73 1.9139374627313843E-4 0.0 0.0 2.0995043327290963 4.253194361625299E-5 74-75 2.1265971808126494E-4 0.0 0.0 2.341702485651849 4.253194361625299E-5 76-77 2.1265971808126494E-4 0.0 0.0 2.6088030915619176 4.253194361625299E-5 78-79 2.1265971808126494E-4 0.0 0.0 2.9179677897084604 4.253194361625299E-5 80-81 2.551916616975179E-4 0.0 0.0 3.2631783100697778 4.253194361625299E-5 82-83 2.551916616975179E-4 0.0 0.0 3.6174268684495487 4.253194361625299E-5 84-85 2.551916616975179E-4 0.0 0.0 3.97769369685102 4.253194361625299E-5 86-87 2.551916616975179E-4 0.0 0.0 4.363841212942981 4.253194361625299E-5 88-89 2.551916616975179E-4 0.0 0.0 4.790798128934736 4.253194361625299E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9890 0.0 22.754608 1 TACATGG 10005 0.0 21.864603 2 GAGTACT 7125 0.0 21.57925 12-13 GTATCAA 16110 0.0 21.027565 1 GTACTTT 7395 0.0 20.663027 14-15 ACATGGG 10015 0.0 20.610857 3 GGTATCA 12630 0.0 19.62632 1 AGTACTT 7450 0.0 19.268385 12-13 AGAGTAC 13275 0.0 18.874508 10-11 ATCAACG 18275 0.0 18.461626 3 TCAACGC 18415 0.0 18.269735 4 CAACGCA 18520 0.0 18.166155 5 TATCAAC 18645 0.0 18.120716 2 AACGCAG 18980 0.0 17.800882 6 GTGTAGG 1040 0.0 17.384201 1 CATGGGG 4885 0.0 17.193586 4 ACTTTTT 9030 0.0 17.052368 16-17 GGACCGA 285 8.682364E-7 16.649948 6 GTGTAGC 1245 0.0 16.432499 1 TACCTGG 2030 0.0 16.36288 2 >>END_MODULE