##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939285_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1008837 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.912628105432294 33.0 31.0 34.0 30.0 34.0 2 31.926822668082156 33.0 31.0 34.0 30.0 34.0 3 31.98642198888423 34.0 31.0 34.0 30.0 34.0 4 35.41581543896586 37.0 35.0 37.0 33.0 37.0 5 35.48898583220084 37.0 35.0 37.0 33.0 37.0 6 35.61037015890575 37.0 35.0 37.0 33.0 37.0 7 35.577995255923405 37.0 35.0 37.0 33.0 37.0 8 35.584172666149236 37.0 35.0 37.0 33.0 37.0 9 37.17096319821735 39.0 37.0 39.0 34.0 39.0 10-11 37.075986011615356 39.0 37.0 39.0 33.0 39.0 12-13 36.991011431975636 39.0 37.0 39.0 33.0 39.0 14-15 38.034044647450486 40.0 38.0 41.0 33.0 41.0 16-17 37.98986258434216 40.0 38.0 41.0 33.0 41.0 18-19 38.06431217332433 40.0 38.0 41.0 33.0 41.0 20-21 38.05772438956937 40.0 38.0 41.0 33.0 41.0 22-23 38.10086565024875 40.0 38.0 41.0 33.0 41.0 24-25 38.01884496702639 40.0 38.0 41.0 33.0 41.0 26-27 37.78146469647723 40.0 37.5 41.0 32.5 41.0 28-29 37.5745819195767 40.0 37.0 41.0 32.5 41.0 30-31 37.46382765501265 40.0 37.0 41.0 32.0 41.0 32-33 37.25889167427444 40.0 36.0 41.0 32.0 41.0 34-35 37.018068330166315 40.0 36.0 41.0 31.0 41.0 36-37 36.686880041077 39.0 35.0 41.0 30.0 41.0 38-39 35.92919321951911 38.5 35.0 40.5 28.5 41.0 40-41 35.86455294561956 38.0 35.0 40.0 30.0 41.0 42-43 36.18493572301571 39.0 35.0 40.5 30.0 41.0 44-45 36.1417558039604 39.0 35.0 41.0 30.0 41.0 46-47 35.80274365432672 39.0 35.0 41.0 28.0 41.0 48-49 35.562147304272145 39.0 35.0 41.0 27.0 41.0 50-51 34.41556217704148 38.0 34.0 40.0 24.5 40.5 52-53 34.40178244850259 38.0 34.0 39.5 24.5 40.5 54-55 34.913370544498264 38.5 35.0 40.0 25.0 41.0 56-57 34.975292837197685 39.0 35.0 40.0 25.0 41.0 58-59 34.84743719748582 39.0 35.0 40.5 24.5 41.0 60-61 34.490561904450374 38.5 34.5 40.0 22.5 41.0 62-63 33.96869712351946 38.0 34.0 40.0 22.0 41.0 64-65 33.38755567053944 37.0 33.0 40.0 20.0 41.0 66-67 33.01380104020768 36.5 32.5 39.0 19.0 41.0 68-69 32.710752083835146 36.0 32.5 39.0 18.0 40.5 70-71 32.1775262009621 35.5 32.0 38.5 13.0 40.0 72-73 31.565988360855123 35.0 31.0 37.5 8.0 40.0 74-75 31.09444290802181 35.0 31.0 37.0 7.0 39.0 76-77 30.479562605257343 35.0 30.5 36.5 2.0 39.0 78-79 30.058472280457597 34.0 29.5 36.0 2.0 39.0 80-81 29.732571763327474 34.0 29.5 36.0 2.0 37.5 82-83 29.306092560046867 34.0 29.0 35.0 2.0 37.0 84-85 28.885577154684057 34.0 29.0 35.0 2.0 36.5 86-87 28.28086945661192 34.0 28.5 35.0 2.0 36.0 88-89 27.640767041652914 34.0 26.5 35.0 2.0 36.0 90-91 27.06664010142372 33.0 25.5 35.0 2.0 35.0 92-93 26.665925218841103 33.0 25.0 35.0 2.0 35.0 94-95 26.23938951485721 33.0 24.5 35.0 2.0 35.0 96-97 25.43343671970794 32.5 20.0 35.0 2.0 35.0 98-99 24.522718734542845 32.0 8.0 35.0 2.0 35.0 100-101 21.92515639295545 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.42483345660580696 1101 2 0.43329032668359346 1101 3 0.4579041730317144 1101 4 0.3630603932264407 1101 5 0.2738775237206994 1101 6 0.4726846315931894 1101 7 0.3542331749034986 1101 8 0.2793764586323917 1101 9 0.41152460605856334 1101 10-11 0.5083868152045028 1101 12-13 0.5281165598736024 1101 14-15 0.6705658828892709 1101 16-17 0.6810104262744474 1101 18-19 0.7722809223132074 1101 20-21 0.8243805434460612 1101 22-23 0.8514523571107304 1101 24-25 0.8914105300200461 1101 26-27 0.7868264104951308 1101 28-29 0.8395862742074982 1101 30-31 0.7044614570270866 1101 32-33 0.582239508368815 1101 34-35 0.5162925698105525 1101 36-37 0.5904212712617891 1101 38-39 1.0041338346605713 1101 40-41 0.769082988595315 1101 42-43 0.6353891322520226 1101 44-45 0.5106649506010044 1101 46-47 0.6206258919899099 1101 48-49 0.5725084794143669 1101 50-51 0.5100020075398604 1101 52-53 0.7431132674970371 1101 54-55 0.9173836077873929 1101 56-57 0.9818505909817574 1101 58-59 0.9629240551523779 1101 60-61 0.8650902700003016 1101 62-63 0.9405627278313275 1101 64-65 0.7847018033589421 1101 66-67 0.5864851404981692 1101 68-69 0.6530389568033854 1101 70-71 0.8944915554723423 1101 72-73 0.8303987838631386 1101 74-75 0.6700525737339333 1101 76-77 0.8681324794759853 1101 78-79 0.7088612655825095 1101 80-81 0.5754593126963954 1101 82-83 0.42160499778686145 1101 84-85 0.6081104174925152 1101 86-87 0.4037255696726696 1101 88-89 0.5196701001640776 1101 90-91 0.8543803466812641 1101 92-93 0.5761517918753754 1101 94-95 0.6773929859617205 1101 96-97 0.5143088575772303 1101 98-99 0.4617781187311394 1101 100-101 0.03778905321025405 1102 1 0.4462658891641844 1102 2 0.23453500847656628 1102 3 0.3665568317857293 1102 4 0.19913496229440142 1102 5 0.15404602875115359 1102 6 0.28368615540804143 1102 7 0.3463543065340744 1102 8 0.3988502998108103 1102 9 0.42601249708948785 1102 10-11 0.5093601694145704 1102 12-13 0.39504837964404516 1102 14-15 0.5570841989573623 1102 16-17 0.5094880560200536 1102 18-19 0.3306472250351007 1102 20-21 0.32371940818070044 1102 22-23 0.5126528622530202 1102 24-25 0.5501593009492041 1102 26-27 0.33122907041413896 1102 28-29 0.4491326014409154 1102 30-31 0.4445479230348397 1102 32-33 0.41046200397678234 1102 34-35 0.5096223848557742 1102 36-37 0.4552735730610067 1102 38-39 1.018345039126693 1102 40-41 0.8769214316013745 1102 42-43 0.7485149098869002 1102 44-45 0.79507602179158 1102 46-47 0.8571259176198254 1102 48-49 0.8727897094638166 1102 50-51 0.7940565899353658 1102 52-53 0.7805317760224852 1102 54-55 0.6099790093149338 1102 56-57 0.7856204017397985 1102 58-59 0.8520636123606735 1102 60-61 0.7070946690254303 1102 62-63 0.8353245560363476 1102 64-65 0.7471714552115145 1102 66-67 0.7490143236495257 1102 68-69 0.6702739085492482 1102 70-71 0.6962063131764609 1102 72-73 0.5833904937511605 1102 74-75 0.6657613107384002 1102 76-77 0.7149075373762876 1102 78-79 0.4144941380433984 1102 80-81 0.14909055882278466 1102 82-83 0.23667527034933755 1102 84-85 0.2869553704965746 1102 86-87 0.30955253047754283 1102 88-89 0.25025369329153335 1102 90-91 0.1856021246516555 1102 92-93 0.5387670682357424 1102 94-95 0.5324505833670408 1102 96-97 0.9372670854784175 1102 98-99 1.1163853286587724 1102 100-101 0.7680033425859563 1103 1 0.36711648854260304 1103 2 0.44439145364453836 1103 3 0.32076173040937306 1103 4 0.19648030786196102 1103 5 0.2776215800313082 1103 6 0.4481914832162346 1103 7 0.3923991489197505 1103 8 0.34280194255167373 1103 9 0.5040519376696295 1103 10-11 0.45214172927840934 1103 12-13 0.555777530894602 1103 14-15 0.6535836624766773 1103 16-17 0.5527764976361595 1103 18-19 0.6514719253458949 1103 20-21 0.6940598884234177 1103 22-23 0.7433187457812167 1103 24-25 0.6110105478043195 1103 26-27 0.5538408063916407 1103 28-29 0.5754483665733616 1103 30-31 0.5402102916895615 1103 32-33 0.6850337443941257 1103 34-35 0.6731287486266808 1103 36-37 0.674795920256507 1103 38-39 0.9498836287374743 1103 40-41 0.6912066173346929 1103 42-43 0.5259193608266628 1103 44-45 0.6413465560523477 1103 46-47 0.4194039256115403 1103 48-49 0.5031545163307669 1103 50-51 0.36415287896896587 1103 52-53 0.7960735290193668 1103 54-55 0.9900709510050376 1103 56-57 0.7621008109450642 1103 58-59 0.6887514915970243 1103 60-61 1.054523724079452 1103 62-63 0.8675735200736412 1103 64-65 0.778692007971614 1103 66-67 0.8381348653100318 1103 68-69 0.8595868432835942 1103 70-71 0.6096548665285368 1103 72-73 0.8729651940619476 1103 74-75 0.714527867642353 1103 76-77 0.669598043518068 1103 78-79 1.053007901547911 1103 80-81 0.9332542039315577 1103 82-83 0.7908760066228311 1103 84-85 0.8875269999179132 1103 86-87 0.8574496186823666 1103 88-89 0.7827801473391851 1103 90-91 0.6371870550941594 1103 92-93 0.9906896115241821 1103 94-95 0.977588495902193 1103 96-97 0.7240345118510696 1103 98-99 0.5326203441982109 1103 100-101 0.5496834521020162 1104 1 0.30551655005434597 1104 2 0.25875263611731114 1104 3 0.3289007288534904 1104 4 0.30058666889969743 1104 5 0.46982768054872537 1104 6 0.2635183095722127 1104 7 0.23871477652043183 1104 8 0.3160251125773641 1104 9 0.47878463296940765 1104 10-11 0.4573069063960489 1104 12-13 0.4635715434502359 1104 14-15 0.5152212542920012 1104 16-17 0.4072831372859085 1104 18-19 0.43814151683556446 1104 20-21 0.4188937841067357 1104 22-23 0.5893273800396344 1104 24-25 0.43949059719462724 1104 26-27 0.5483703320275666 1104 28-29 0.538680966891782 1104 30-31 0.6441102944033119 1104 32-33 0.6793880067959748 1104 34-35 0.8110620445705266 1104 36-37 0.6123954245442391 1104 38-39 1.0998445054607018 1104 40-41 0.8007698839699984 1104 42-43 0.7961125122179098 1104 44-45 0.8064558298519628 1104 46-47 0.8527518830598879 1104 48-49 0.9038628059425236 1104 50-51 0.6539630745401155 1104 52-53 1.0272847449617686 1104 54-55 1.062567739730298 1104 56-57 1.0729629477544123 1104 58-59 1.2193581059184027 1104 60-61 1.3835906226212629 1104 62-63 1.2209573442947956 1104 64-65 0.9660139179111908 1104 66-67 1.0797380603022475 1104 68-69 1.072338317756227 1104 70-71 1.2299719459872378 1104 72-73 1.2562048275243356 1104 74-75 1.0790974437540868 1104 76-77 0.9436565972684754 1104 78-79 0.7993692106625083 1104 80-81 0.87265270051223 1104 82-83 1.1794182009637524 1104 84-85 0.9486972608000634 1104 86-87 1.2273336283433274 1104 88-89 1.1203020835117101 1104 90-91 0.9666915463558716 1104 92-93 0.9908744859678649 1104 94-95 1.1497518321904998 1104 96-97 1.1355819844421582 1104 98-99 1.2737599396680537 1104 100-101 0.4975726043254838 1105 1 0.20872022179993976 1105 2 0.32469923363884234 1105 3 0.21622696266928187 1105 4 0.2705933030172716 1105 5 0.17150160282982085 1105 6 0.31989638489763195 1105 7 0.28688475735520313 1105 8 0.35971381203015795 1105 9 0.5150771585302749 1105 10-11 0.41730274381247057 1105 12-13 0.3775795043913277 1105 14-15 0.47860948892295596 1105 16-17 0.398387782902617 1105 18-19 0.48919278598994964 1105 20-21 0.5968767522022702 1105 22-23 0.6190356523072893 1105 24-25 0.40322788180260716 1105 26-27 0.20028566247599855 1105 28-29 0.4341643469096468 1105 30-31 0.5024279819146997 1105 32-33 0.37304136764861084 1105 34-35 0.3478402048820257 1105 36-37 0.29984935428329607 1105 38-39 0.7071773733940674 1105 40-41 0.585175696844523 1105 42-43 0.2636161761586706 1105 44-45 0.2082459575912523 1105 46-47 0.193752030484724 1105 48-49 0.18755534277704555 1105 50-51 -0.05609238321399346 1105 52-53 -0.029700373801944124 1105 54-55 0.17399977688135237 1105 56-57 0.03813654049763926 1105 58-59 0.11599215845367183 1105 60-61 0.18516546674117507 1105 62-63 0.127841758888124 1105 64-65 0.1942172644549487 1105 66-67 0.20166436089281348 1105 68-69 0.05804081877518996 1105 70-71 0.05919978673372839 1105 72-73 0.15102696379691594 1105 74-75 0.10037052224480192 1105 76-77 0.2551192033926739 1105 78-79 0.2023381950788341 1105 80-81 0.34864142251235464 1105 82-83 0.2654102400226641 1105 84-85 0.54731255135707 1105 86-87 0.3141636041483089 1105 88-89 0.32550065175639986 1105 90-91 0.489695782651868 1105 92-93 0.4571998352346398 1105 94-95 0.46964909008856637 1105 96-97 0.518794414957366 1105 98-99 0.4677336243350041 1105 100-101 0.505511323290385 1106 1 0.15682893127229036 1106 2 0.3125749942418743 1106 3 0.279731639810322 1106 4 0.14643337203916218 1106 5 0.23976288700019666 1106 6 0.21360494906073768 1106 7 0.1691956283024325 1106 8 0.22224900411080029 1106 9 0.14154160317653464 1106 10-11 0.3510962230604946 1106 12-13 0.35401216264216373 1106 14-15 0.5545732978413156 1106 16-17 0.5195166540314844 1106 18-19 0.49472134833669656 1106 20-21 0.5940386811823757 1106 22-23 0.5729680835124498 1106 24-25 0.47562707351998057 1106 26-27 0.43855307687977785 1106 28-29 0.5097592065954117 1106 30-31 0.42695232121176474 1106 32-33 0.5424855598200793 1106 34-35 0.381215920075924 1106 36-37 0.4474176950334581 1106 38-39 0.8330855561204302 1106 40-41 0.47237372608800854 1106 42-43 0.3495983757772265 1106 44-45 0.43421317219482347 1106 46-47 0.5182684231829313 1106 48-49 0.4171155552918009 1106 50-51 0.0996496020734483 1106 52-53 0.46692799782643135 1106 54-55 0.3428593264227189 1106 56-57 0.5411165150685306 1106 58-59 0.6179731320255613 1106 60-61 0.6214110393503844 1106 62-63 0.2687746895937764 1106 64-65 0.40555706102201583 1106 66-67 0.04395275431757284 1106 68-69 0.2441785446856457 1106 70-71 0.3595585189106387 1106 72-73 0.09536671495484939 1106 74-75 0.15112090822246316 1106 76-77 0.24044637621984677 1106 78-79 0.39331199400717765 1106 80-81 0.21713088759272736 1106 82-83 0.3554111482053912 1106 84-85 0.5893273547355093 1106 86-87 0.6165089860391468 1106 88-89 0.49794752306690526 1106 90-91 0.6546614987539492 1106 92-93 0.4720826796629396 1106 94-95 0.7724428872005475 1106 96-97 0.8953610301603483 1106 98-99 0.648637947284783 1106 100-101 0.3993617910044911 1107 1 0.23380606042332985 1107 2 0.2236851401346307 1107 3 0.1823246989251217 1107 4 0.11573942830173678 1107 5 0.15891426702114586 1107 6 0.1636191860314966 1107 7 0.20911995518328297 1107 8 0.23975034335127532 1107 9 0.3851134902266864 1107 10-11 0.4394730636112456 1107 12-13 0.41904954072302303 1107 14-15 0.3882907880805391 1107 16-17 0.3810623547076233 1107 18-19 0.41912351621712673 1107 20-21 0.38926905467361905 1107 22-23 0.42340942960596806 1107 24-25 0.5432980498866087 1107 26-27 0.565982652135439 1107 28-29 0.5305900665567052 1107 30-31 0.6699605207417036 1107 32-33 0.6803496281189254 1107 34-35 0.6477643474939114 1107 36-37 0.6316445792603389 1107 38-39 0.9616579499102187 1107 40-41 0.6771303221489546 1107 42-43 0.46133446577419335 1107 44-45 0.5000463728106368 1107 46-47 0.6707053582285596 1107 48-49 0.44916900908438606 1107 50-51 0.3534566645761572 1107 52-53 0.37158421117828766 1107 54-55 0.3546147207868131 1107 56-57 0.3303297173252062 1107 58-59 0.2604031657599464 1107 60-61 0.3350756356236744 1107 62-63 0.2539272182245682 1107 64-65 0.15747084771841457 1107 66-67 0.27703416565984895 1107 68-69 0.30764406467289973 1107 70-71 0.25053807075976664 1107 72-73 0.12766388638245374 1107 74-75 0.3137558578139341 1107 76-77 0.3361304023386573 1107 78-79 0.38835897601068226 1107 80-81 0.3801297245046058 1107 82-83 0.4398822326764744 1107 84-85 0.3450378278372881 1107 86-87 0.3220657481176481 1107 88-89 0.2943337455400865 1107 90-91 0.15461323783612002 1107 92-93 0.09648098666994542 1107 94-95 0.14402975443958965 1107 96-97 0.6529971138833837 1107 98-99 0.5176153466969637 1107 100-101 0.5833145532181234 1108 1 0.2696064450206741 1108 2 0.2051212829046669 1108 3 0.18810772973980505 1108 4 0.18249051877592137 1108 5 0.32432395699816396 1108 6 0.11602291943987098 1108 7 0.35958194101884544 1108 8 0.3622633586882671 1108 9 0.4014448067690637 1108 10-11 0.4226305140122548 1108 12-13 0.37894354194248336 1108 14-15 0.3235775945155126 1108 16-17 0.4044336787543372 1108 18-19 0.4857245199223925 1108 20-21 0.2523008074056676 1108 22-23 0.3454979807996281 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-1.4585752491844133 2316 80-81 -1.277293166613937 2316 82-83 -1.2208386889140144 2316 84-85 -1.0816158325120249 2316 86-87 -0.9167023678243815 2316 88-89 -1.2766528765334968 2316 90-91 -1.3697271560694908 2316 92-93 -1.5999051151509427 2316 94-95 -1.5104481348209085 2316 96-97 -1.2123410503678222 2316 98-99 -1.5918606845182026 2316 100-101 -0.9718322323634432 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3903.0 3 811.0 4 760.0 5 859.0 6 1092.0 7 1151.0 8 1440.0 9 1800.0 10 2267.0 11 3060.0 12 4179.0 13 5840.0 14 6124.0 15 6458.0 16 8121.0 17 10055.0 18 9226.0 19 6809.0 20 6664.0 21 7106.0 22 7929.0 23 8986.0 24 9736.0 25 11271.0 26 12686.0 27 14853.0 28 17354.0 29 20272.0 30 24135.0 31 30060.0 32 36987.0 33 47348.0 34 64538.0 35 99082.0 36 132500.0 37 187400.0 38 169356.0 39 26601.0 40 18.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.718636950615405 17.305633344964466 13.37321718107088 20.602512523349247 2 12.475818784881527 22.864605056704203 41.424258674452325 23.235317483961946 3 23.084691834691835 28.439947884392332 29.52049271493716 18.954867565978677 4 11.186115418119764 15.87443643302207 40.527455979943525 32.411992168914644 5 11.958558261425884 41.327295955367376 33.71368284691909 13.000462936287649 6 26.359683159041698 36.519572332186826 20.4011519324242 16.719592576347278 7 25.545753573675988 36.66975680541853 20.35213686072341 17.432352760182074 8 21.9255360650872 33.089170299001275 21.694046081595257 23.291247554316268 9 25.964066934491992 20.88749648839109 21.97269531742463 31.17574125969229 10-11 22.482836109035002 25.57367644241697 28.4175175410648 23.525969907483226 12-13 30.592219688718075 23.2333522725863 26.323841376726552 19.850586661969068 14-15 26.04365035051802 24.922737142502637 29.337551957317455 19.69606054966189 16-17 19.250066984886224 28.658069445960564 32.02428326171739 20.06758030743582 18-19 18.360869401214604 31.755071937232852 31.82023930768727 18.063819353865274 20-21 20.367667428234405 29.650453316493454 28.333596456475956 21.64828279879619 22-23 19.003212704256946 29.000725353255973 26.712362432361182 25.2836995101259 24-25 20.579591536747888 28.78048998507221 29.43063841874684 21.209280059433063 26-27 23.525317090463503 25.641504319063557 30.810627507951466 20.022551082521474 28-29 19.7890425241417 26.568180125611303 33.537869572772635 20.104907777474367 30-31 19.330649617175784 31.535799512602196 30.91178338051928 18.221767489702742 32-33 18.647822598582824 30.704309125861904 29.177705268342287 21.47016300721299 34-35 20.21839686455515 27.598032388986155 29.46999840840363 22.71357233805507 36-37 22.636445435021514 27.51968498055777 30.825088621625618 19.018780962795102 38-39 24.589588642879896 24.81517800254858 31.916464240203883 18.678769114367633 40-41 20.411182057582607 27.06572771121396 34.06723031202235 18.45585991918108 42-43 18.50564847432448 30.806587795790673 31.20197104173678 19.48579268814806 44-45 19.316676922586723 31.667891402750975 27.98611567482958 21.02931599983272 46-47 19.01190963988288 29.189695405769662 26.35176478847083 25.44663016587662 48-49 21.024723545626923 28.331344906706608 28.129033286062555 22.514898261603914 50-51 23.8350020438779 29.57394232430492 28.058086960158573 18.532968671658608 52-53 19.71564976910797 29.398276550591596 31.19327908507602 19.692794595224413 54-55 17.672205644121444 28.822546615329316 32.20627140093415 21.298976339615088 56-57 20.564498671015496 31.826951835571197 27.017525874265775 20.591023619147528 58-59 19.418679638859253 29.35970366713906 28.513678126144487 22.707938567857198 60-61 19.92541058745877 26.787029718877097 30.270687544672327 23.016872148991805 62-63 24.99065147865973 25.98087275106444 28.550857200900843 20.477618569374993 64-65 23.08094467990185 27.127979509894523 30.798492718523946 18.99258309167968 66-67 19.397289005588604 28.155162377321304 32.02889363721515 20.418654979874944 68-69 18.851928749541198 31.55044500410616 30.2534563703741 19.344169875978544 70-71 19.202384331535622 30.650462375069093 27.330723914526594 22.816429378868687 72-73 19.17178425798536 28.875613774847892 26.589266676924257 25.363335290242496 74-75 20.530306878243646 28.96335846083824 28.45144654952449 22.054888111393627 76-77 22.52637919833125 26.116738064952944 30.124733345447396 21.23214939126842 78-79 19.989367675343757 26.265624937779 32.47428530469375 21.27072208218349 80-81 18.973877811072065 31.097450498252883 30.002936755233893 19.92573493544116 82-83 18.833208296349103 30.059903252737165 28.402146819472318 22.704741631441415 84-85 20.0074376536443 27.002814758614786 28.578910319022572 24.410837268718343 86-87 21.76776260699781 27.092935381637552 29.912390488110134 21.226911523254504 88-89 23.669679002289747 24.28441055324844 30.990794709933038 21.055115734528776 90-91 20.112750316957765 26.300871347516175 32.848626151181634 20.737752184344426 92-93 18.48748409522695 29.43933029389282 31.111155377494033 20.9620302333862 94-95 18.4823833123516 30.43733964910044 28.704055981387345 22.376221057160613 96-97 18.53701387173624 28.05461049981276 27.168703737640133 26.23967189081087 98-99 20.529223886632014 26.900645657324567 28.251317358053484 24.318813097989928 100-101 22.710584959881466 27.02703644622882 29.22334940464455 21.039029189245166 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3736.0 1 2348.0 2 1877.5 3 2479.5 4 1689.0 5 1419.0 6 2516.5 7 4659.0 8 6063.5 9 5591.0 10 3973.5 11 2756.5 12 2450.0 13 2355.5 14 2228.5 15 1999.5 16 1823.0 17 1670.5 18 1583.5 19 1633.5 20 1759.0 21 1967.0 22 2292.0 23 2783.0 24 3341.5 25 4073.0 26 5076.5 27 6353.5 28 7745.0 29 9322.5 30 11188.0 31 13223.0 32 15346.5 33 17756.5 34 20437.0 35 22818.5 36 24726.0 37 26128.0 38 26920.0 39 26833.5 40 26404.0 41 26151.0 42 26212.0 43 26346.0 44 28843.0 45 31201.5 46 35791.5 47 50710.5 48 124813.0 49 125035.0 50 53519.5 51 36498.0 52 23211.5 53 17437.5 54 14545.5 55 12176.5 56 10187.5 57 8532.5 58 6835.0 59 5346.0 60 4188.0 61 3389.5 62 2529.0 63 1548.0 64 937.5 65 666.0 66 504.5 67 408.5 68 341.5 69 268.0 70 209.5 71 162.0 72 139.5 73 159.5 74 145.5 75 94.5 76 67.0 77 49.5 78 33.5 79 24.0 80 19.0 81 12.0 82 14.0 83 14.0 84 8.5 85 6.5 86 10.0 87 8.5 88 3.0 89 2.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2890457031215152 2 0.18407334386030647 3 0.1828838553700945 4 0.20617800497007943 5 0.21995624664836838 6 0.11290228252928868 7 0.1319340983726806 8 0.1437298592339496 9 0.1451175958058636 10-11 0.13322271090374363 12-13 0.12187300822630416 14-15 0.13872409517097412 16-17 0.11369527485609668 18-19 0.1418465024577806 20-21 0.16890736561010353 22-23 0.17262451714201602 24-25 0.19725684129348942 26-27 0.17743203312328948 28-29 0.1746565599794615 30-31 0.16370335346542603 32-33 0.1749539321020145 34-35 0.35258421330700596 36-37 0.46672554634693214 38-39 0.4318834459878057 40-41 0.4208311154329193 42-43 0.4455625636252437 44-45 0.4499240214226877 46-47 0.4526499325460902 48-49 0.46518912371374166 50-51 0.45780438267034224 52-53 0.4646935035094867 54-55 0.4460581838294987 56-57 0.4086388584082464 58-59 0.39857776826186986 60-61 0.35689610908402447 62-63 0.4626614606720412 64-65 0.4604311697528937 66-67 0.46989751565416416 68-69 0.4814950284337311 70-71 0.4740111633494806 72-73 0.4661308021018261 74-75 0.4716321863690567 76-77 0.44551300160481827 78-79 0.4314869498244018 80-81 0.4289097247622758 82-83 0.4428366525018412 84-85 0.44486869533928675 86-87 0.4454634395843927 88-89 0.4537402969954512 90-91 0.4718304344507586 92-93 0.47723269467713814 94-95 0.4747050316354377 96-97 0.47391203930862963 98-99 0.47024444979714264 100-101 0.45230299840311167 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1008837.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.252584282429254 #Duplication Level Percentage of deduplicated Percentage of total 1 79.5037834278711 39.157667940532235 2 12.892279098022208 12.699561257358793 3 3.9580127996067485 5.8482707701069545 4 1.5919132996667105 3.1362337584861892 5 0.7500517307605735 1.8470993042733537 6 0.3742490535508757 1.105963983158033 7 0.22603420742080835 0.7792938196193822 8 0.13072354806620196 0.5150778055063043 9 0.09822273896231885 0.4353951356273391 >10 0.3962263544279321 3.431236983052345 >50 0.032980303104414285 1.1170177030963324 >100 0.030146632644768 3.347575286130123 >500 0.004451180653923302 1.5932198269159326 >1k 0.008497708521126303 9.280894130597227 >5k 0.0012139583601609007 4.076721486060221 >10k+ 0.0012139583601609007 11.628770809479281 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 33380 3.3087604836063704 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 28497 2.824737792130939 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 20143 1.996655554861687 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 12223 1.2115931513217695 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 11814 1.1710514186137106 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 10638 1.054481546572935 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 8681 0.8604957986275286 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 7954 0.7884326209288518 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7411 0.7346082667467589 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 6422 0.6365745903451202 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5389 0.5341794561460375 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 5053 0.5008737784201016 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 4985 0.4941333436422336 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 4891 0.48481568380223966 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 4599 0.4558714638737477 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4401 0.4362449037852497 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 4150 0.4113647695316488 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 3883 0.3848986506244319 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 3727 0.3694353002516759 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 3590 0.35585530665508897 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3579 0.35476494220572796 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3077 0.30500467369852613 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2890 0.2864684780593892 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2619 0.25960586298876825 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 2511 0.2489004665768603 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 2506 0.24840484637260526 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 2505 0.24830572233175427 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2388 0.23670820955218733 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 2018 0.20003231443731745 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1928 0.19111115076072743 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1906 0.18893042186200545 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 1856 0.18397421981945547 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 1814 0.1798110101037135 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1799 0.1783241494909485 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 1552 0.15384051140075156 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 1465 0.14521671984671458 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1452 0.1439281073156516 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 1447 0.14343248711139658 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1415 0.1402605178041646 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1403 0.1390710293139526 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1382 0.1369894244560816 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1372 0.1359981840475716 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1352 0.13401570323055162 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 1242 0.12311205873694164 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 1228 0.12172432216502765 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1217 0.12063395771566665 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1216 0.12053483367481564 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1209 0.11984096538885866 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1208 0.11974184134800765 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 1144 0.11339790273354367 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 1064 0.1054679794654637 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1053 0.1043776150161027 No Hit GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1048 0.1038819948118477 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 1043 0.1033863746075927 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 9.912404085099972E-5 0.0 10-11 0.0 0.0 0.0 9.912404085099972E-5 0.0 12-13 0.0 0.0 0.0 1.9824808170199943E-4 0.0 14-15 0.0 0.0 0.0 1.9824808170199943E-4 0.0 16-17 0.0 0.0 0.0 1.9824808170199943E-4 0.0 18-19 0.0 0.0 0.0 2.973721225529992E-4 0.0 20-21 0.0 0.0 0.0 4.956202042549986E-4 0.0 22-23 0.0 0.0 0.0 0.001090364449360997 0.0 24-25 0.0 0.0 0.0 0.0022302909191474936 0.0 26-27 0.0 0.0 0.0 0.0041632097157419876 0.0 28-29 0.0 0.0 0.0 0.009367221860419474 0.0 30-31 0.0 0.0 0.0 0.021262106762539437 0.0 32-33 0.0 0.0 0.0 0.042623337565929884 0.0 34-35 0.0 0.0 0.0 0.08846820645951725 0.0 36-37 0.0 0.0 0.0 0.15250233684926306 0.0 38-39 0.0 0.0 0.0 0.22600281314027934 0.0 40-41 0.0 0.0 0.0 0.31095211614958607 0.0 42-43 0.0 0.0 0.0 0.3902513488303859 0.0 44-45 0.0 0.0 0.0 0.4739616013290552 0.0 46-47 0.0 0.0 0.0 0.5911262176149368 0.0 48-49 0.0 0.0 0.0 0.7366403095842045 0.0 50-51 0.0 0.0 0.0 0.87204374938667 0.0 52-53 0.0 0.0 0.0 0.9945610638785056 0.0 54-55 0.0 0.0 0.0 1.1269412204350158 0.0 56-57 0.0 0.0 0.0 1.2787992510187474 0.0 58-59 0.0 0.0 0.0 1.5159535187547641 0.0 60-61 0.0 0.0 0.0 1.8624416035494336 0.0 62-63 0.0 0.0 0.0 2.1443999377501024 0.0 64-65 0.0 0.0 0.0 2.3612833391320898 0.0 66-67 0.0 0.0 0.0 2.6097873095455464 0.0 68-69 0.0 0.0 0.0 2.8884249883777064 0.0 70-71 0.0 0.0 0.0 3.2347148250906734 0.0 72-73 0.0 0.0 0.0 3.6291293836368013 0.0 74-75 0.0 0.0 0.0 4.012392487587192 0.0 76-77 0.0 0.0 0.0 4.36418370856739 0.0 78-79 0.0 0.0 0.0 4.758895639236071 0.0 80-81 0.0 0.0 0.0 5.190134778958345 0.0 82-83 0.0 0.0 0.0 5.650565948711239 0.0 84-85 0.0 0.0 0.0 6.359897585040993 0.0 86-87 0.0 0.0 0.0 7.1634961842200475 0.0 88-89 0.0 0.0 0.0 7.739456423584781 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGGGTC 120 5.474196E-6 27.647087 9 TACCTGG 750 0.0 27.186632 2 GCTTATG 375 0.0 25.310005 1 GTAGCCC 450 0.0 24.236147 3 TAGCCCT 850 0.0 23.988205 4 ACCTGGG 845 0.0 23.56898 3 GTGTAGC 535 0.0 22.175823 1 AGTACTC 490 0.0 21.289959 5 AGTACTT 4825 0.0 20.381296 12-13 CTAATAC 210 8.748448E-7 20.322235 3 GAGTACT 4730 0.0 20.139374 12-13 GTCTTAG 355 3.6379788E-12 20.051939 1 TGTATAG 595 0.0 19.92376 5 TAGGGAC 195 1.04784685E-5 19.453762 3 GCTTATA 2950 0.0 19.143373 1 TGTAGCC 695 0.0 19.103874 2 ATAGCCC 555 0.0 18.796541 3 GTAGGAC 585 0.0 18.64319 3 GTATAGC 535 0.0 18.627691 1 CATGGGG 1740 0.0 18.531385 4 >>END_MODULE