##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939284_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1026835 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.861702220902092 33.0 31.0 34.0 30.0 34.0 2 31.883613238738455 33.0 31.0 34.0 30.0 34.0 3 31.949194369105065 34.0 31.0 34.0 30.0 34.0 4 35.40266060272585 37.0 35.0 37.0 33.0 37.0 5 35.47686337142774 37.0 35.0 37.0 33.0 37.0 6 35.60301314232569 37.0 35.0 37.0 33.0 37.0 7 35.549965671213 37.0 35.0 37.0 33.0 37.0 8 35.553634225557175 37.0 35.0 37.0 33.0 37.0 9 37.13571898114108 39.0 37.0 39.0 33.0 39.0 10-11 37.05957529690749 39.0 37.0 39.0 33.0 39.0 12-13 36.969378721995255 39.0 37.0 39.0 33.0 39.0 14-15 38.011902593892884 40.0 38.0 41.0 33.0 41.0 16-17 37.97612274610819 40.0 38.0 41.0 33.0 41.0 18-19 38.070437801594224 40.0 38.0 41.0 33.0 41.0 20-21 37.98934444190157 40.0 38.0 41.0 33.0 41.0 22-23 37.94385173859481 40.0 38.0 41.0 33.0 41.0 24-25 37.98245385091081 40.0 38.0 41.0 33.0 41.0 26-27 37.75156962900563 40.0 37.5 41.0 32.5 41.0 28-29 37.55835260777047 40.0 37.0 41.0 32.0 41.0 30-31 37.45124630539473 40.0 37.0 41.0 32.0 41.0 32-33 37.26636460580327 40.0 36.0 41.0 32.0 41.0 34-35 37.00289481757049 40.0 36.0 41.0 31.0 41.0 36-37 36.66945468356649 39.0 35.0 41.0 30.0 41.0 38-39 36.20298490020305 38.5 35.0 40.5 30.0 41.0 40-41 36.02382709977747 39.0 35.0 40.0 30.0 41.0 42-43 35.99914494539044 39.0 35.0 40.0 30.0 41.0 44-45 36.018894953911776 39.0 35.0 41.0 29.5 41.0 46-47 35.757039836000914 39.0 35.0 41.0 27.5 41.0 48-49 35.55985966586647 39.0 35.0 41.0 27.0 41.0 50-51 34.399158092585466 38.0 34.0 40.0 24.5 40.5 52-53 34.39478056357643 38.0 33.5 39.5 24.5 40.5 54-55 34.9400604770971 38.5 35.0 40.0 25.0 41.0 56-57 34.98421947050889 39.0 35.0 40.5 25.0 41.0 58-59 34.86343132051401 39.0 35.0 41.0 24.5 41.0 60-61 34.52182970000049 38.5 34.5 40.0 23.0 41.0 62-63 34.033774170144184 38.0 34.0 40.0 22.0 41.0 64-65 33.476020977080054 37.0 33.0 40.0 20.0 41.0 66-67 33.11535105445373 36.5 33.0 39.5 19.5 41.0 68-69 32.79552264969543 36.0 33.0 39.0 18.5 41.0 70-71 32.26993333885191 36.0 32.0 39.0 14.5 40.0 72-73 31.659461354550636 35.0 31.0 38.0 9.5 40.0 74-75 31.191553170665202 35.0 31.0 37.0 7.5 39.0 76-77 30.602117185331625 35.0 30.5 36.5 3.5 39.0 78-79 30.157490249163693 34.5 30.0 36.0 2.0 39.0 80-81 29.7615361766983 34.0 29.5 36.0 2.0 37.5 82-83 29.323774024064235 34.0 29.0 35.0 2.0 37.0 84-85 28.8971996474604 34.0 29.0 35.0 2.0 36.5 86-87 28.416878076808835 34.0 29.0 35.0 2.0 36.0 88-89 27.807943827391938 34.0 27.0 35.0 2.0 36.0 90-91 27.26796077266552 33.5 25.5 35.0 2.0 35.5 92-93 26.908978073400302 33.0 25.0 35.0 2.0 35.0 94-95 26.478710308861697 33.0 25.0 35.0 2.0 35.0 96-97 25.72244907896595 33.0 22.0 35.0 2.0 35.0 98-99 24.87309840432007 32.5 10.0 35.0 2.0 35.0 100-101 22.51894413415982 29.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.41882793396267104 1101 2 0.2662025303448239 1101 3 0.5572302904022877 1101 4 0.4577178008289664 1101 5 0.516386126668614 1101 6 0.4776989130917286 1101 7 0.5056728938116564 1101 8 0.5200726491090251 1101 9 0.5543809991001254 1101 10-11 0.7299502256125976 1101 12-13 0.7180174372779362 1101 14-15 0.8125224415695556 1101 16-17 0.8164206631438304 1101 18-19 0.8174730290037147 1101 20-21 0.8576502648516282 1101 22-23 0.8866045658114459 1101 24-25 0.8499172545829268 1101 26-27 0.8405380971596159 1101 28-29 0.8270165157484755 1101 30-31 0.8360254389939854 1101 32-33 0.8975984502349164 1101 34-35 1.096894806140618 1101 36-37 0.9340406341206133 1101 38-39 1.0740240470499458 1101 40-41 0.8720064792447246 1101 42-43 0.9192270605871613 1101 44-45 0.948040600147003 1101 46-47 1.1669295283973256 1101 48-49 1.1997895900069153 1101 50-51 1.221888439174144 1101 52-53 1.1326643920636101 1101 54-55 1.2606389017206823 1101 56-57 1.3026351586048506 1101 58-59 1.1433231138014435 1101 60-61 1.2259791240387372 1101 62-63 1.563641440897925 1101 64-65 1.1314778431411199 1101 66-67 1.2667729655510556 1101 68-69 1.2911684084480655 1101 70-71 1.1877886411423901 1101 72-73 1.439368555355717 1101 74-75 1.3930498774992373 1101 76-77 1.0508853066359585 1101 78-79 0.9365259039194989 1101 80-81 0.9928746758130025 1101 82-83 0.8779686252521941 1101 84-85 1.1365227794235473 1101 86-87 1.0640349509742286 1101 88-89 1.2583819067429474 1101 90-91 1.2433859367322846 1101 92-93 1.2278644198215503 1101 94-95 1.1032557046083262 1101 96-97 0.7895577929779201 1101 98-99 1.048969908618659 1101 100-101 0.5280409596804603 1102 1 0.3366959139931076 1102 2 0.41240511191195495 1102 3 0.4479533055093796 1102 4 0.33190352147779123 1102 5 0.30862077831480406 1102 6 0.2493898302811388 1102 7 0.2827679812248931 1102 8 0.28637679520605275 1102 9 0.34908168764174974 1102 10-11 0.4405870106242915 1102 12-13 0.3803211750753448 1102 14-15 0.5053541154124019 1102 16-17 0.5205919309141436 1102 18-19 0.5462878465680276 1102 20-21 0.5208552776573896 1102 22-23 0.47716994478772534 1102 24-25 0.47168565590433076 1102 26-27 0.401744181751873 1102 28-29 0.5310752691355063 1102 30-31 0.47560243131053426 1102 32-33 0.6567944711695475 1102 34-35 0.6236488705777248 1102 36-37 0.6599120986171059 1102 38-39 0.7130593464078032 1102 40-41 0.5092925859222817 1102 42-43 0.5010302369725963 1102 44-45 0.4996429441579906 1102 46-47 0.606080705970534 1102 48-49 0.39963659364450166 1102 50-51 0.46062946122231807 1102 52-53 0.49476351607534497 1102 54-55 0.5902747263003221 1102 56-57 0.5503425330527207 1102 58-59 0.650212589878798 1102 60-61 0.5593087842221038 1102 62-63 0.6479239935129044 1102 64-65 0.5801243067096564 1102 66-67 0.7447416053565661 1102 68-69 0.4804489195649495 1102 70-71 0.5666553081183778 1102 72-73 0.5333534436300944 1102 74-75 0.31749820903852566 1102 76-77 0.42415409305813867 1102 78-79 0.38843180477692485 1102 80-81 0.37303003845729776 1102 82-83 0.43471879228385646 1102 84-85 0.4221759196187911 1102 86-87 0.5784976889943501 1102 88-89 0.41860528839173483 1102 90-91 0.45264123070801077 1102 92-93 0.5774680676074517 1102 94-95 0.7015781963495371 1102 96-97 0.6317656499616966 1102 98-99 0.5592058176420807 1102 100-101 0.4170571199937356 1103 1 0.2462237102808622 1103 2 0.3804642982703861 1103 3 0.4685409545146797 1103 4 0.49477080210610325 1103 5 0.30237750597895285 1103 6 0.31612711962338125 1103 7 0.3688080425071547 1103 8 0.27588363250621484 1103 9 0.4694158923677776 1103 10-11 0.43797852792969394 1103 12-13 0.47967133255252037 1103 14-15 0.4610839495060617 1103 16-17 0.5036977339338335 1103 18-19 0.5187136785202284 1103 20-21 0.698387584683779 1103 22-23 0.6096723226076506 1103 24-25 0.5553017647088154 1103 26-27 0.6025779849538111 1103 28-29 0.6970693117711022 1103 30-31 0.5797708777825292 1103 32-33 0.6404373482462233 1103 34-35 0.6704682872572008 1103 36-37 0.5882078709387102 1103 38-39 0.7478700605511222 1103 40-41 0.696513868407294 1103 42-43 0.7127906210688337 1103 44-45 0.8649250646607811 1103 46-47 1.0018655574066244 1103 48-49 1.0269681386294351 1103 50-51 1.0794936891285332 1103 52-53 0.99682467303424 1103 54-55 1.2034310473145524 1103 56-57 1.1836325815203779 1103 58-59 1.1140167600338842 1103 60-61 1.1664821116322415 1103 62-63 0.952609010684462 1103 64-65 0.8764234506944817 1103 66-67 0.7641896094116731 1103 68-69 0.7824986857697169 1103 70-71 0.5570386639484539 1103 72-73 0.6127054236279328 1103 74-75 0.7584599304092876 1103 76-77 0.7657360409910048 1103 78-79 0.7332982766616958 1103 80-81 0.7963600115181642 1103 82-83 0.6247997235919129 1103 84-85 0.5913588038716391 1103 86-87 0.6475376192829359 1103 88-89 0.4995542521178784 1103 90-91 0.6674799661064874 1103 92-93 0.6514521547239411 1103 94-95 0.6236865110305096 1103 96-97 0.31917544942747256 1103 98-99 0.4015156347635198 1103 100-101 0.1880750547361103 1104 1 0.30208942821554885 1104 2 0.3862064488401238 1104 3 0.33149230712120215 1104 4 0.33696283740158606 1104 5 0.42714239626108963 1104 6 0.41304374067793503 1104 7 0.4510425385347503 1104 8 0.42602876196168893 1104 9 0.4133478225066014 1104 10-11 0.4052539089981835 1104 12-13 0.45743749997385663 1104 14-15 0.5315173475256643 1104 16-17 0.6237123298104947 1104 18-19 0.5405660279587821 1104 20-21 0.6412513620303173 1104 22-23 0.6524581447038145 1104 24-25 0.6974224792433219 1104 26-27 0.5520878620499019 1104 28-29 0.590910846991946 1104 30-31 0.643072199704541 1104 32-33 0.5757593697751417 1104 34-35 0.8166244299650671 1104 36-37 0.6970533039220825 1104 38-39 0.839698028241159 1104 40-41 0.7843628010838088 1104 42-43 1.0084218098034654 1104 44-45 0.9644656262703108 1104 46-47 0.9805887499229229 1104 48-49 0.8430269202054603 1104 50-51 0.7181495415774819 1104 52-53 0.7604726470270364 1104 54-55 0.8846919320237134 1104 56-57 0.8360566580823914 1104 58-59 0.8832493154733427 1104 60-61 0.9294633194410338 1104 62-63 0.9525194450776553 1104 64-65 1.069288057352189 1104 66-67 1.2400782163347586 1104 68-69 1.049691135720792 1104 70-71 0.7944206944339314 1104 72-73 0.6520612147078602 1104 74-75 0.5059678503310039 1104 76-77 0.6582357507320928 1104 78-79 0.38249965000100516 1104 80-81 0.580404064527734 1104 82-83 0.4191454800066339 1104 84-85 0.3698365202803906 1104 86-87 0.6404619363451829 1104 88-89 0.4554332390313469 1104 90-91 0.3803210203268499 1104 92-93 0.5587650206574963 1104 94-95 0.4929174078476173 1104 96-97 0.17662349308240977 1104 98-99 0.0973143443553326 1104 100-101 0.0661646535153615 1105 1 0.27044475672413526 1105 2 0.33840080620689506 1105 3 0.4586644113048024 1105 4 0.26555269511021606 1105 5 0.23077256770734778 1105 6 0.18411653761280178 1105 7 0.10807905955594777 1105 8 0.3168301345638298 1105 9 0.4475757983054578 1105 10-11 0.4099802915981243 1105 12-13 0.2775108387253553 1105 14-15 0.3044848901292241 1105 16-17 0.5573720431637028 1105 18-19 0.4641134433644396 1105 20-21 0.5805598363004805 1105 22-23 0.6020885483572087 1105 24-25 0.46710364595514164 1105 26-27 0.4376161977698061 1105 28-29 0.4145737209421725 1105 30-31 0.5395739348078052 1105 32-33 0.49677185735851026 1105 34-35 0.6443219027774845 1105 36-37 0.6858122213208446 1105 38-39 0.8216648871237311 1105 40-41 0.7806555497711969 1105 42-43 0.8367030255367922 1105 44-45 0.9591490505514315 1105 46-47 1.0241759630503608 1105 48-49 0.9800839528563614 1105 50-51 0.8641497737846109 1105 52-53 0.7629769364087267 1105 54-55 0.8796656152157851 1105 56-57 0.7797819054474857 1105 58-59 0.7031114102396501 1105 60-61 0.727710800638711 1105 62-63 0.7150134786444866 1105 64-65 0.7692071073670306 1105 66-67 0.8551949004169614 1105 68-69 0.8138331948820152 1105 70-71 0.7701485992806099 1105 72-73 0.8938194741864329 1105 74-75 0.8830190133017055 1105 76-77 0.8566825430486169 1105 78-79 0.7406468951214258 1105 80-81 0.7045316752453843 1105 82-83 0.6693470486947959 1105 84-85 0.7320554529016228 1105 86-87 0.46082392145812534 1105 88-89 0.5205125119650269 1105 90-91 0.6271507479994902 1105 92-93 0.7467931306204711 1105 94-95 0.9217023840463838 1105 96-97 1.196541704571505 1105 98-99 1.047841325533657 1105 100-101 0.8772431911264675 1106 1 0.21728048133763522 1106 2 0.25456697237305903 1106 3 0.16503745101117318 1106 4 0.29797400530930673 1106 5 0.16003516363661419 1106 6 0.21639881989508325 1106 7 0.3239059976051095 1106 8 0.2724802291028112 1106 9 0.38087020382208436 1106 10-11 0.39600798595915165 1106 12-13 0.3905822434634345 1106 14-15 0.5132075016296156 1106 16-17 0.39993683017292625 1106 18-19 0.3554075152140612 1106 20-21 0.5912232775194752 1106 22-23 0.5493691623044867 1106 24-25 0.5198091292161777 1106 26-27 0.49678647916231 1106 28-29 0.5127135274153147 1106 30-31 0.39694519495684233 1106 32-33 0.4925037003125752 1106 34-35 0.6210903378792594 1106 36-37 0.5384009211317604 1106 38-39 0.7413206540017256 1106 40-41 0.6675563394497601 1106 42-43 0.617080625358831 1106 44-45 0.7194510191867352 1106 46-47 0.5457809013219688 1106 48-49 0.591389703051 1106 50-51 0.6794650890999279 1106 52-53 0.6193471955567631 1106 54-55 0.4983259422094477 1106 56-57 0.8051684030562001 1106 58-59 0.9295322755493984 1106 60-61 0.9302744754246035 1106 62-63 0.9286993867748379 1106 64-65 0.8324509067219381 1106 66-67 0.923763468985527 1106 68-69 0.6943247975958329 1106 70-71 0.8831504900602738 1106 72-73 1.0256040365265484 1106 74-75 0.9317761037254684 1106 76-77 0.9126829879379521 1106 78-79 0.8441948875583059 1106 80-81 0.737286652444805 1106 82-83 0.8966715954637863 1106 84-85 0.9732549857678201 1106 86-87 0.7496683214136368 1106 88-89 0.4332307857944109 1106 90-91 0.5394519380784608 1106 92-93 0.5797232829950687 1106 94-95 0.5714771588211569 1106 96-97 0.2346909649429847 1106 98-99 0.4510222842701701 1106 100-101 -0.014384547723494734 1107 1 0.15079007789070786 1107 2 0.19314388921084813 1107 3 0.1347611272565885 1107 4 0.0940388448524061 1107 5 0.12589529254022125 1107 6 -0.0878421799980913 1107 7 0.1839260967556413 1107 8 0.10041883965011777 1107 9 0.3164985198851866 1107 10-11 0.27614800740352763 1107 12-13 0.33860487083388335 1107 14-15 0.42794981311105573 1107 16-17 0.44261537828625563 1107 18-19 0.48278241976287717 1107 20-21 0.40445498749031117 1107 22-23 0.4141341248955399 1107 24-25 0.22572650904660208 1107 26-27 0.17134641459180955 1107 28-29 0.2819857640788541 1107 30-31 0.4235058465175001 1107 32-33 0.4876386574040552 1107 34-35 0.48748786188547655 1107 36-37 0.6624058549193066 1107 38-39 0.8302084548460442 1107 40-41 0.6365900965269944 1107 42-43 0.7577573692964421 1107 44-45 0.7586537594764309 1107 46-47 0.7011205294876888 1107 48-49 0.7998496452065922 1107 50-51 0.8685232183754508 1107 52-53 0.9155371602249858 1107 54-55 1.1178140073496934 1107 56-57 1.10608641593074 1107 58-59 1.0332708955923664 1107 60-61 0.9776721695614299 1107 62-63 1.1625280771252733 1107 64-65 1.2086274785506887 1107 66-67 1.1188093053357093 1107 68-69 1.198968769631115 1107 70-71 1.08822265708897 1107 72-73 1.2048870745052405 1107 74-75 1.2020913794755188 1107 76-77 1.1268764303922616 1107 78-79 1.3010801324870336 1107 80-81 1.3928785091236193 1107 82-83 1.2688342719742884 1107 84-85 0.9692584002494975 1107 86-87 0.9090094807547935 1107 88-89 1.0149943675579962 1107 90-91 0.8905658978880702 1107 92-93 0.837222382885475 1107 94-95 0.7823011250799077 1107 96-97 0.9516450732014441 1107 98-99 1.045777612721153 1107 100-101 0.6641724677900456 1108 1 0.0999995640567164 1108 2 0.18600846170928875 1108 3 0.14608835837787382 1108 4 0.26429056109954985 1108 5 0.13299951554833456 1108 6 0.1393265322964794 1108 7 0.0451909188900288 1108 8 0.0032272440603478003 1108 9 0.07055510403330345 1108 10-11 0.13688235314931063 1108 12-13 0.15799846351122682 1108 14-15 0.10673595831596572 1108 16-17 0.119279617936769 1108 18-19 -0.012604515955864315 1108 20-21 0.12678180255168314 1108 22-23 0.06825311803055456 1108 24-25 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Count 2 3246.0 3 834.0 4 732.0 5 792.0 6 906.0 7 1112.0 8 1406.0 9 1695.0 10 2379.0 11 3110.0 12 4318.0 13 6378.0 14 5848.0 15 6570.0 16 8201.0 17 10736.0 18 9234.0 19 6995.0 20 6970.0 21 7238.0 22 7932.0 23 8963.0 24 10153.0 25 11285.0 26 13207.0 27 15079.0 28 17783.0 29 20891.0 30 24943.0 31 30122.0 32 37541.0 33 47604.0 34 64447.0 35 98547.0 36 131788.0 37 188563.0 38 177429.0 39 31836.0 40 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.57333161445576 17.386934967829642 13.392107226708234 20.647626191006363 2 12.550660178784002 22.926137776187545 41.320962029117474 23.202240015910977 3 23.159314193893113 28.26902601024185 29.458383223023493 19.11327657284155 4 11.12073168353517 15.960334639911075 40.50849275531894 32.41044092123481 5 11.996450581168578 41.32284109524924 33.687787658943755 12.992920664638428 6 26.36928102027612 36.529264337888506 20.452880050514892 16.648574591320482 7 25.584418621875205 36.59433142197812 20.371720258676138 17.44952969747054 8 21.888756672153498 33.07770348301762 21.706122154619514 23.32741769020937 9 25.90164892994971 20.895022024714457 22.046349354851287 31.156979690484544 10-11 22.454195123923977 25.608535008943416 28.430328789429858 23.50694107770275 12-13 30.553486008263135 23.189947408864526 26.3125247594466 19.94404182342574 14-15 26.015676655698755 24.95192099207202 29.305416081362303 19.726986270866924 16-17 19.269769760258903 28.64302632264943 31.9703518765662 20.11685204052547 18-19 18.297966270010463 31.77607481389838 31.8463395345279 18.07961938156326 20-21 20.35710961663003 29.678146211058394 28.284578608800654 21.680165563510926 22-23 18.99792004959132 29.081829576621903 26.659233166924306 25.261017206862473 24-25 20.600822600006726 28.79675131970326 29.409095583508428 21.193330496781588 26-27 23.527115329034938 25.690853715298918 30.744921587329593 20.037109368336555 28-29 19.807983313067613 26.565459901896045 33.48303162183434 20.143525163202 30-31 19.371657838706863 31.49405726989678 30.884165374027233 18.250119517369125 32-33 18.65464541213746 30.7020514652244 29.179886586850916 21.463416535787225 34-35 20.197153015183787 27.571404027013358 29.5074493694735 22.723993588329353 36-37 22.60735193430673 27.556974094398644 30.817886686635028 19.017787284659597 38-39 24.617927425142796 24.742610039002404 31.898815564025156 18.740646971829644 40-41 20.433622551811663 27.120411738212496 33.99023802149941 18.45572768847643 42-43 18.588830919838216 30.82465996249547 31.141220490099602 19.445288627566708 44-45 19.311798679343845 31.707573322413378 27.9989268996832 20.981701098559576 46-47 18.952469288441858 29.205081566809916 26.35823325830353 25.484215886444694 48-49 21.011274175354774 28.41001284399724 27.99703043655906 22.581682544088928 50-51 23.825437101213563 29.540203146503192 28.02300645877643 18.61135329350682 52-53 19.703491713484794 29.427636183081358 31.17632742584361 19.692544677590245 54-55 17.70746090119532 28.82043428674183 32.19339334526275 21.2787114668001 56-57 20.564252357565337 31.797172436063725 27.08179748998618 20.556777716384754 58-59 19.385744885074455 29.49294034031931 28.459037854234698 22.662276920371532 60-61 19.883321053137845 26.828540639146333 30.234769017579477 23.05336929013634 62-63 24.945643304769806 25.981083381460383 28.58952071319893 20.483752600570877 64-65 23.06094152364317 27.23994664995853 30.747140059498296 18.9519717669 66-67 19.495993411550803 28.169383750284087 32.00588463860059 20.328738199564516 68-69 18.871282550368047 31.589981576808192 30.176200794704833 19.362535078118928 70-71 19.253369544973946 30.546337261963945 27.33480272037017 22.865490472691935 72-73 19.13777944165674 28.898791792536883 26.52279544047204 25.440633325334332 74-75 20.515727942757717 28.935455807315595 28.429502844519167 22.11931340540752 76-77 22.500360398662007 26.111188484835157 30.145210118528396 21.24324099797444 78-79 19.970705176623945 26.279938518002442 32.4788032703107 21.270553035062914 80-81 19.005395243215883 31.111445507945806 29.98393103781849 19.89922821101982 82-83 18.910921229374303 30.13456389989529 28.275814216894197 22.678700653836213 84-85 20.018442115262733 27.080296500738125 28.53382251687473 24.367438867124413 86-87 21.771397298829232 27.122236772667748 29.865674413150128 21.240691515352893 88-89 23.70669348674732 24.257169777756925 30.920503482505367 21.115633252990378 90-91 20.122927060084095 26.351547537694923 32.803018527045616 20.722506875175362 92-93 18.529192240558903 29.495143628453242 31.09456177301675 20.881102357971113 94-95 18.384541351825213 30.514533907563084 28.757091722791905 22.343833017819794 96-97 18.527976099115154 28.076158652290424 27.14585425035512 26.250010998239304 98-99 20.498577455020357 26.915352586363216 28.282325202433455 24.303744756182976 100-101 22.700842889485163 27.009161884722193 29.23412820383764 21.055867021955002 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3414.0 1 2191.0 2 1871.0 3 2441.0 4 1609.0 5 1413.5 6 2565.0 7 4637.0 8 6008.0 9 5519.5 10 3890.5 11 2683.0 12 2455.5 13 2325.0 14 2194.5 15 2107.0 16 1916.0 17 1737.0 18 1705.0 19 1700.0 20 1812.5 21 2019.0 22 2341.5 23 2819.5 24 3388.5 25 4199.5 26 5159.5 27 6409.5 28 7901.5 29 9539.5 30 11482.5 31 13607.5 32 15681.5 33 17947.5 34 20748.0 35 23289.0 36 25108.5 37 26572.5 38 27575.0 39 27478.0 40 26944.0 41 26766.0 42 26617.0 43 26896.5 44 29555.5 45 31682.0 46 36471.0 47 51532.0 48 127223.0 49 127435.5 50 54162.5 51 37245.5 52 23953.5 53 17823.5 54 14703.5 55 12510.5 56 10556.5 57 8546.0 58 6868.0 59 5456.0 60 4266.0 61 3406.0 62 2529.0 63 1588.0 64 951.0 65 712.5 66 539.5 67 407.0 68 330.5 69 256.5 70 197.0 71 154.0 72 140.5 73 168.0 74 141.5 75 86.0 76 60.5 77 39.0 78 32.5 79 28.0 80 18.5 81 14.0 82 13.5 83 11.5 84 7.5 85 7.5 86 5.0 87 2.5 88 4.0 89 3.5 90 2.0 91 1.5 92 1.5 93 1.0 94 1.5 95 1.0 96 0.0 97 0.0 98 1.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2834924793175145 2 0.1098521184026645 3 0.12066203430930968 4 0.12222022038594321 5 0.12806341817331898 6 0.058724137763126495 7 0.06690461466545258 8 0.07226087930388037 9 0.06963144029956128 10-11 0.06442125560581788 12-13 0.06929058709529769 14-15 0.06422648234623868 16-17 0.06481080212497625 18-19 0.06968013361445607 20-21 0.07435469184435668 22-23 0.08268124869136716 24-25 0.0916895119469048 26-27 0.09237121835543198 28-29 0.08594370078931864 30-31 0.08117175592962841 32-33 0.07435469184435668 34-35 0.23888940287387941 36-37 0.3619374096130342 38-39 0.3346691532719473 40-41 0.3186490526715587 42-43 0.34007411122526987 44-45 0.35365954608091854 46-47 0.35896711740445153 48-49 0.36899794027277993 50-51 0.36334951574498336 52-53 0.36295996922582496 54-55 0.3500075474638087 56-57 0.3286311822249923 58-59 0.31528921394381765 60-61 0.27545808235987285 62-63 0.34104797752316585 64-65 0.36817015391956837 66-67 0.37323425866862736 68-69 0.38365462805611417 70-71 0.3856023606519061 72-73 0.36914402021746434 74-75 0.37152999264730946 76-77 0.35667853160439605 78-79 0.33559432625494845 80-81 0.33437699338257854 82-83 0.3473294151445948 84-85 0.3538543193404977 86-87 0.3481572014978064 88-89 0.3623756494470874 90-91 0.38487196092848414 92-93 0.3798565494943199 94-95 0.3797591628645303 96-97 0.38409286789016733 98-99 0.37440289822610257 100-101 0.3533673861915497 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1026835.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.21043618166111 #Duplication Level Percentage of deduplicated Percentage of total 1 79.2740544886825 39.01110799276835 2 13.050482421454676 12.844398646817709 3 4.0164135949240976 5.9294839467650515 4 1.5948282972428254 3.1392878456870124 5 0.7481966496906828 1.8409541740467998 6 0.3788830452416419 1.1186999950906342 7 0.23510058021446145 0.8098581469240678 8 0.13360209396539754 0.5259693855056389 9 0.09567767397343628 0.42375060621716065 >10 0.39541700375744626 3.4147993562342176 >50 0.03161440891695269 1.0875545639548 >100 0.03061904463030107 3.379484524031181 >500 0.004572974184574771 1.6627376931613855 >1k 0.007952998581869167 8.648799481405241 >5k 0.0013917747518271042 4.557999025298525 >10k+ 0.001192949787280375 11.605114616092264 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 33762 3.287967394956347 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 28787 2.8034689117531055 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 20846 2.0301216845939223 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 12246 1.1925966684033948 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 12242 1.1922071218842365 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 10727 1.0446663777529983 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 8896 0.8663514586082477 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 8228 0.8012971899087975 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7421 0.7227061796685933 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 6518 0.6347660529685879 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5407 0.5265695072723466 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 5080 0.49472407933114865 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 5035 0.4903416809906168 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 4828 0.4701826486241704 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 4634 0.4512896424449887 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4461 0.43444175549138864 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 4207 0.4097055515248311 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 3915 0.3812686556262691 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3783 0.36841362049404236 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 3749 0.3651024750811961 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 3703 0.36062269011087467 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3114 0.3032619651648025 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2997 0.2918677294794198 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2655 0.2585615020913779 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 2609 0.2540817171210564 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 2554 0.24872545248262865 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 2488 0.2422979349165153 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2447 0.23830508309514187 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 2011 0.19584451250687793 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1996 0.194383713060034 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1991 0.193896779911086 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1974 0.19224120720466287 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 1901 0.18513198323002233 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1811 0.1763671865489587 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 1548 0.1507545029142949 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 1505 0.14656687783334224 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1494 0.1454956249056567 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1483 0.14442437197797114 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1430 0.13926288059912256 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 1408 0.13712037474375144 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1397 0.13604912181606588 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1311 0.12767387165416058 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 1292 0.12582352568815827 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1291 0.12572613905836866 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1276 0.12426533961152474 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1219 0.11871430171351775 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1202 0.1170587290070946 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1200 0.11686395574751543 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 1196 0.11647440922835704 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 1118 0.10887825210476854 No Hit GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1097 0.10683313287918701 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1069 0.10410630724507833 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1031 0.10040561531307367 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 9.738662978959618E-5 0.0 18-19 0.0 0.0 0.0 9.738662978959618E-5 0.0 20-21 0.0 0.0 0.0 2.4346657447399048E-4 0.0 22-23 0.0 0.0 0.0 9.738662978959618E-4 0.0 24-25 0.0 0.0 0.0 0.002726825634108693 0.0 26-27 0.0 0.0 0.0 0.004918024804374607 0.0 28-29 0.0 0.0 0.0 0.00944650308959083 0.0 30-31 0.0 0.0 0.0 0.021571138498395558 0.0 32-33 0.0 0.0 0.0 0.044943929647898645 0.0 34-35 0.0 0.0 0.0 0.09383201780227593 0.0 36-37 0.0 0.0 0.0 0.1656546572721031 0.0 38-39 0.0 0.0 0.0 0.24098321541435574 0.0 40-41 0.0 0.0 0.0 0.32220366465887895 0.0 42-43 0.0 0.0 0.0 0.40050251500971434 0.0 44-45 0.0 0.0 0.0 0.4863488291692434 0.0 46-47 0.0 0.0 0.0 0.6042353445295496 0.0 48-49 0.0 0.0 0.0 0.7441312382223044 0.0 50-51 0.0 0.0 0.0 0.8813003062809507 0.0 52-53 0.0 0.0 0.0 1.010970603845798 0.0 54-55 0.0 0.0 0.0 1.1463380192533368 0.0 56-57 0.0 0.0 0.0 1.2935866034952062 0.0 58-59 0.0 0.0 0.0 1.5210817706837028 0.0 60-61 0.0 0.0 0.0 1.865684360194189 0.0 62-63 0.0 0.0 0.0 2.152147131720286 0.0 64-65 0.0 0.0 0.0 2.370439262393666 0.0 66-67 0.0 0.0 0.0 2.614782316535763 0.0 68-69 0.0 0.0 0.0 2.8916525050275848 0.0 70-71 0.0 0.0 0.0 3.2339665087380154 0.0 72-73 0.0 0.0 0.0 3.6304274786114616 0.0 74-75 0.0 0.0 0.0 4.002687870982193 0.0 76-77 0.0 0.0 0.0 4.352744111760897 0.0 78-79 0.0 0.0 0.0 4.749594628153501 0.0 80-81 0.0 4.869331489479809E-5 0.0 5.185594569721523 0.0 82-83 0.0 9.738662978959618E-5 0.0 5.647742821388052 0.0 84-85 0.0 9.738662978959618E-5 0.0 6.348050076205038 0.0 86-87 0.0 9.738662978959618E-5 0.0 7.14949334605852 0.0 88-89 0.0 9.738662978959618E-5 0.0 7.73313141838757 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACGTGG 230 0.0 30.90827 7 TACCTGG 785 0.0 29.000164 2 AGAGGCG 85 6.464297E-4 27.898441 5 ACCTGGG 820 0.0 26.027205 3 ACGTGGA 265 1.8189894E-12 25.037642 8 CCGTATC 95 1.180706E-8 25.022785 94-95 GGACGTG 475 0.0 24.943516 6 AGTACTC 640 0.0 23.713676 5 TAGCCCT 730 0.0 22.739141 4 AGGACGT 525 0.0 22.584454 5 TAGGACG 505 0.0 22.539732 4 GCTTATG 390 0.0 21.924837 1 AAGTACT 630 0.0 21.83164 4 GTCTAGG 240 5.9208105E-9 21.772581 1 GTACTAG 175 4.0457144E-6 21.716026 1 GTATAGC 615 0.0 21.627752 1 GAGTACT 4955 0.0 21.18666 12-13 TACTAGA 225 7.266317E-8 21.078823 2 GTAGGAC 590 0.0 20.900188 3 ATGGGAG 1165 0.0 20.762188 5 >>END_MODULE