##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939284_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1026835 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.184979086221254 33.0 31.0 34.0 31.0 34.0 2 32.35930504900982 34.0 31.0 34.0 30.0 34.0 3 32.465933669966454 34.0 31.0 34.0 30.0 34.0 4 35.95095122390647 37.0 35.0 37.0 35.0 37.0 5 35.91128175412798 37.0 35.0 37.0 35.0 37.0 6 35.98849669128925 37.0 35.0 37.0 35.0 37.0 7 35.94846981257943 37.0 35.0 37.0 35.0 37.0 8 35.96295315216174 37.0 35.0 37.0 35.0 37.0 9 37.62341174580142 39.0 37.0 39.0 35.0 39.0 10-11 37.52361430999139 39.0 37.0 39.0 34.5 39.0 12-13 37.44307459328908 39.0 37.0 39.0 34.0 39.0 14-15 38.52109150934668 40.0 38.0 41.0 34.0 41.0 16-17 38.49122936012115 40.0 38.0 41.0 34.0 41.0 18-19 38.78910827932433 40.0 38.0 41.0 34.0 41.0 20-21 38.81339553092756 40.0 38.0 41.0 34.0 41.0 22-23 38.79087584665501 40.0 38.0 41.0 34.5 41.0 24-25 38.70768234429095 40.0 38.0 41.0 34.0 41.0 26-27 38.354555503074984 40.0 37.5 41.0 33.5 41.0 28-29 38.15188224008726 40.0 37.5 41.0 33.5 41.0 30-31 38.04181781883165 40.0 37.0 41.0 33.0 41.0 32-33 37.86507277215911 40.0 37.0 41.0 33.0 41.0 34-35 37.85774004586911 40.0 37.0 41.0 33.0 41.0 36-37 37.66984228235306 40.0 37.0 41.0 33.0 41.0 38-39 37.4428359960461 40.0 36.0 41.0 32.5 41.0 40-41 37.210573753329406 40.0 36.0 41.0 32.0 41.0 42-43 37.17304532860683 40.0 36.0 41.0 32.0 41.0 44-45 37.035794942712315 40.0 36.0 41.0 31.5 41.0 46-47 36.708336295509994 40.0 36.0 41.0 31.0 41.0 48-49 36.420486738375686 39.5 35.5 41.0 30.0 41.0 50-51 36.232084512117325 39.0 35.0 41.0 30.0 41.0 52-53 35.822779219640935 39.0 35.0 41.0 28.0 41.0 54-55 35.61350168235403 39.0 35.0 40.0 28.0 41.0 56-57 35.5545725457352 39.0 35.0 40.0 28.0 41.0 58-59 35.38652363816972 39.0 35.0 40.0 28.0 41.0 60-61 35.013867856082044 38.0 34.5 40.0 26.5 41.0 62-63 34.50288313117492 38.0 34.0 40.0 26.0 41.0 64-65 33.8984052939372 37.0 33.0 39.5 24.0 41.0 66-67 33.535095219777276 36.5 33.0 39.0 23.5 40.5 68-69 33.28129592388261 36.0 33.0 39.0 23.5 40.0 70-71 32.85403399767246 36.0 32.5 38.5 22.5 40.0 72-73 32.25462367371584 35.0 32.0 37.5 21.5 39.5 74-75 31.740955460224864 35.0 31.0 37.0 20.0 39.0 76-77 29.56284651380212 32.5 28.5 35.0 13.5 37.5 78-79 30.471235885025344 34.0 30.0 36.0 13.5 38.0 80-81 30.62139292096588 34.0 31.0 36.0 15.0 37.0 82-83 30.35056703365195 34.0 31.0 35.0 9.5 37.0 84-85 30.012460619281576 34.0 30.5 35.0 6.5 36.5 86-87 29.36266634853701 34.0 29.5 35.0 2.0 36.0 88-89 28.684594409033586 34.0 29.0 35.0 2.0 36.0 90-91 28.127333018449896 33.5 27.5 35.0 2.0 35.0 92-93 27.887546197782505 33.0 27.5 35.0 2.0 35.0 94-95 27.59158238665414 33.0 27.0 35.0 2.0 35.0 96-97 26.899033437699337 33.0 26.0 35.0 2.0 35.0 98-99 26.102852454386536 33.0 25.0 35.0 2.0 35.0 100-101 22.877199842233658 29.0 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.32216681985051565 1101 2 0.2638515436604152 1101 3 0.2377698408541562 1101 4 0.13308128777578077 1101 5 0.2489237500511905 1101 6 0.17555395502105142 1101 7 0.2678286665753191 1101 8 0.3305577699541544 1101 9 0.3006472132416391 1101 10-11 0.23334440380195787 1101 12-13 0.341154740834682 1101 14-15 0.40911419947341443 1101 16-17 0.3177159423128444 1101 18-19 0.42229738663962735 1101 20-21 0.4573711467329531 1101 22-23 0.41859741854730714 1101 24-25 0.43594310182652407 1101 26-27 0.4967384674256934 1101 28-29 0.4827005928024235 1101 30-31 0.4574602445039062 1101 32-33 0.4855805517303935 1101 34-35 0.5803986308700999 1101 36-37 0.6911401773643675 1101 38-39 0.7105214603630117 1101 40-41 0.7675705545399083 1101 42-43 0.7990392000308262 1101 44-45 0.8557972903088782 1101 46-47 0.9404309365700882 1101 48-49 0.7210307797695563 1101 50-51 0.7419546393956438 1101 52-53 0.5976334725882708 1101 54-55 0.4319446165987557 1101 56-57 0.7553925017165213 1101 58-59 0.6897134049388285 1101 60-61 0.6810611463938017 1101 62-63 0.9332329925620257 1101 64-65 0.9645688226954263 1101 66-67 0.7519994591888945 1101 68-69 0.8282681171689745 1101 70-71 0.6666380625914456 1101 72-73 0.5477213829607948 1101 74-75 0.5189735176907391 1101 76-77 0.8850371508552186 1101 78-79 0.8947183377568351 1101 80-81 0.9133635738229309 1101 82-83 0.8920165252023864 1101 84-85 0.7908524648490207 1101 86-87 0.8557511172078769 1101 88-89 1.0924352549438723 1101 90-91 1.2609618841447627 1101 92-93 1.1929219485781921 1101 94-95 1.2132291882272206 1101 96-97 1.2912822682992804 1101 98-99 1.601288314842055 1101 100-101 1.4910390245467013 1102 1 0.12030905659005953 1102 2 0.1845822607830172 1102 3 0.19609544124018896 1102 4 0.0953066134875229 1102 5 0.12063995397829075 1102 6 0.14414504295394437 1102 7 0.13733634075222056 1102 8 0.2537986017718339 1102 9 0.3168632037393948 1102 10-11 0.21205076678032242 1102 12-13 0.2288827854506934 1102 14-15 0.4048444526191801 1102 16-17 0.3281142432296633 1102 18-19 0.33808675043458436 1102 20-21 0.3526399749130462 1102 22-23 0.35699289384518806 1102 24-25 0.3184398075909769 1102 26-27 0.4209861289824275 1102 28-29 0.409872468382396 1102 30-31 0.3191058311562216 1102 32-33 0.4135841057929781 1102 34-35 0.4388659761802174 1102 36-37 0.5739025098687378 1102 38-39 0.432048361740101 1102 40-41 0.5845624303054677 1102 42-43 0.666096496530578 1102 44-45 0.7853864852162715 1102 46-47 0.6904871937617258 1102 48-49 0.6599888269838416 1102 50-51 0.810269429121341 1102 52-53 0.7752559856724162 1102 54-55 0.6406553177605616 1102 56-57 0.752311115539321 1102 58-59 0.7116421102578627 1102 60-61 0.6102826396568588 1102 62-63 0.5516723600687996 1102 64-65 0.7832832124730871 1102 66-67 0.7683302839541284 1102 68-69 0.7662030954548555 1102 70-71 0.6810851413932753 1102 72-73 0.8611133784323783 1102 74-75 0.7070111508520966 1102 76-77 0.5943887200248881 1102 78-79 0.5185728949373392 1102 80-81 0.8545620491950388 1102 82-83 0.9244763448081912 1102 84-85 0.7828807627963883 1102 86-87 0.7014724387995024 1102 88-89 0.5974125664764784 1102 90-91 0.7555635136314187 1102 92-93 0.7416986736440769 1102 94-95 1.0034339412059516 1102 96-97 0.8760270578393836 1102 98-99 0.7608093515519023 1102 100-101 0.8075483644004571 1103 1 0.18889734347759202 1103 2 0.16554489341411482 1103 3 0.15505683227907952 1103 4 0.08802792160172856 1103 5 0.21613434118420116 1103 6 0.05805030605188932 1103 7 0.1335796700418399 1103 8 0.12393603122034591 1103 9 0.29863571898876273 1103 10-11 0.2489101080530105 1103 12-13 0.1764870250893722 1103 14-15 0.30177863114646897 1103 16-17 0.1973142136137085 1103 18-19 0.22976979131030362 1103 20-21 0.23618660011737092 1103 22-23 0.2403749226523999 1103 24-25 0.29443037604655586 1103 26-27 0.30218113299766713 1103 28-29 0.40791268913520895 1103 30-31 0.43414675244041234 1103 32-33 0.5246917996778535 1103 34-35 0.6097899372010573 1103 36-37 0.5635904601594817 1103 38-39 0.37991231057283414 1103 40-41 0.4852982730219395 1103 42-43 0.2719872250262583 1103 44-45 0.4441593592744013 1103 46-47 0.3982494231601237 1103 48-49 0.4760196048014862 1103 50-51 0.26406625224001345 1103 52-53 0.42164654045909344 1103 54-55 0.41783678955989956 1103 56-57 0.5229434732545712 1103 58-59 0.3572478420853358 1103 60-61 0.28285484279956563 1103 62-63 0.3676382105879341 1103 64-65 0.2597607356675127 1103 66-67 0.2016425443923282 1103 68-69 0.2529317278239631 1103 70-71 0.13823881975255148 1103 72-73 0.28359417077323457 1103 74-75 0.37418869284125833 1103 76-77 0.471586708417707 1103 78-79 0.5434326462771786 1103 80-81 0.5079112156766072 1103 82-83 0.39004608185263123 1103 84-85 0.3533952262064375 1103 86-87 0.11498585995554933 1103 88-89 0.4713841237634284 1103 90-91 0.6136005449730781 1103 92-93 0.8689333972189495 1103 94-95 1.1049790970029925 1103 96-97 1.0678689997351505 1103 98-99 0.7439504659093821 1103 100-101 0.5652527857216718 1104 1 0.1369526087742372 1104 2 0.12112035243784902 1104 3 0.18160474158560902 1104 4 0.14281221776533215 1104 5 0.0931309078359348 1104 6 0.12729115460307838 1104 7 0.13032213574982165 1104 8 0.07353713254558869 1104 9 0.06227208262512818 1104 10-11 0.0910540433003959 1104 12-13 0.16224277636236906 1104 14-15 0.20688718797915584 1104 16-17 0.12559538327418807 1104 18-19 0.05216285163439949 1104 20-21 0.13285442781968015 1104 22-23 0.20837014582004088 1104 24-25 0.14894597538974352 1104 26-27 0.32083068268170223 1104 28-29 0.20416088538340205 1104 30-31 0.261633441996068 1104 32-33 0.36525009196027725 1104 34-35 0.3457787249139841 1104 36-37 0.3959468649296767 1104 38-39 0.5001309169983301 1104 40-41 0.5158235587196316 1104 42-43 0.6688308666974834 1104 44-45 0.8030935285533971 1104 46-47 0.6246165426306973 1104 48-49 0.6384484913683011 1104 50-51 0.42635249728488134 1104 52-53 0.48355627823090686 1104 54-55 0.7562654107471403 1104 56-57 0.9246551715179905 1104 58-59 0.9590773025354835 1104 60-61 0.7386956813990295 1104 62-63 0.6175328880688156 1104 64-65 0.6730164454749499 1104 66-67 0.5743283515608937 1104 68-69 0.5805212519652088 1104 70-71 0.4510881670846629 1104 72-73 0.6246218531802299 1104 74-75 0.43843211225709666 1104 76-77 0.6939615238959718 1104 78-79 0.7821432332636284 1104 80-81 0.7000178319211514 1104 82-83 0.5691325126632023 1104 84-85 0.46142129974975177 1104 86-87 0.3840459186707825 1104 88-89 0.6087296645572451 1104 90-91 0.5029289687842642 1104 92-93 0.4986625441894752 1104 94-95 0.5877393763149961 1104 96-97 0.6309994835552892 1104 98-99 0.7686407181858392 1104 100-101 0.44253131817261604 1105 1 0.09980269327183322 1105 2 0.1082844583758984 1105 3 0.10455712622381697 1105 4 0.08258994394152808 1105 5 0.13809201314187192 1105 6 0.0553166199848647 1105 7 0.05280365593249314 1105 8 0.057298759027517576 1105 9 0.051516377424981386 1105 10-11 0.04363379999892203 1105 12-13 0.14750407388420683 1105 14-15 0.016593445961284203 1105 16-17 0.06672926358431397 1105 18-19 -0.011544141670299268 1105 20-21 0.04585738201037515 1105 22-23 0.10671825010683733 1105 24-25 0.049045308439268354 1105 26-27 0.21643745280954363 1105 28-29 0.14687506420870022 1105 30-31 0.16180695232283426 1105 32-33 0.14872178204116437 1105 34-35 0.11250892603116114 1105 36-37 0.06644172412185867 1105 38-39 0.2204325927597779 1105 40-41 0.22987499259865274 1105 42-43 0.24434172238076002 1105 44-45 0.2512581247065029 1105 46-47 0.16913419682268227 1105 48-49 0.3885710451307105 1105 50-51 0.4075259818108563 1105 52-53 0.3111086215878487 1105 54-55 0.24856871195848385 1105 56-57 0.3341427677871991 1105 58-59 0.08720081092719312 1105 60-61 0.06003003386364725 1105 62-63 0.20208853392714587 1105 64-65 0.3660805475428859 1105 66-67 0.2859899870731084 1105 68-69 0.4188341381708227 1105 70-71 0.4624598661958146 1105 72-73 0.41625143120827346 1105 74-75 0.6586096217066384 1105 76-77 0.6010399712043011 1105 78-79 0.5893468144135703 1105 80-81 0.7234755895743135 1105 82-83 0.5384440830284092 1105 84-85 0.4967667723557625 1105 86-87 0.5982457071042866 1105 88-89 0.7749996440456144 1105 90-91 0.841040286301709 1105 92-93 0.632013938077268 1105 94-95 0.7686916190488482 1105 96-97 0.6652822860373213 1105 98-99 0.9604260684960941 1105 100-101 0.7103145141167353 1106 1 0.17368212826237794 1106 2 0.15121627908549584 1106 3 0.14587622309846893 1106 4 0.18555401912782088 1106 5 0.1773813024311579 1106 6 0.17585382385540527 1106 7 0.24466218112434746 1106 8 0.2405097477940643 1106 9 0.18011720047024937 1106 10-11 0.27888293802583775 1106 12-13 0.15912680772915877 1106 14-15 0.21529214465998336 1106 16-17 0.2515112678107627 1106 18-19 0.1620878236283403 1106 20-21 0.33979020372097324 1106 22-23 0.2915975738750518 1106 24-25 0.22232970394588136 1106 26-27 0.4196128504293881 1106 28-29 0.5370847183072414 1106 30-31 0.3471311654248268 1106 32-33 0.1419233169093701 1106 34-35 0.36633219374331105 1106 36-37 0.5386083351773578 1106 38-39 0.6666704973310118 1106 40-41 0.5478179355416017 1106 42-43 0.4103827634218007 1106 44-45 0.48419939931444134 1106 46-47 0.6135647384754463 1106 48-49 0.27331690209878445 1106 50-51 0.4826705708443342 1106 52-53 0.3622013810139393 1106 54-55 0.49085266257576876 1106 56-57 0.558950909262002 1106 58-59 0.5209401057775978 1106 60-61 0.5384389983837181 1106 62-63 0.6365785795282974 1106 64-65 0.7028895787963592 1106 66-67 0.6505628933126744 1106 68-69 0.7043974792897103 1106 70-71 0.5844707659844985 1106 72-73 0.7849812721603371 1106 74-75 0.8120408306933982 1106 76-77 0.7249972618282641 1106 78-79 0.8545008573453892 1106 80-81 0.7407011484665418 1106 82-83 0.8369787287852795 1106 84-85 1.061817934629147 1106 86-87 0.9202482652179569 1106 88-89 0.997138449517756 1106 90-91 1.0016314330039684 1106 92-93 0.9479409762265298 1106 94-95 1.0341654261893218 1106 96-97 0.8224950543612017 1106 98-99 1.0075426295015433 1106 100-101 0.9516391721080559 1107 1 0.10954585760436686 1107 2 0.1562845885016344 1107 3 0.24063204611515943 1107 4 0.11353140362694347 1107 5 0.10588589397922732 1107 6 0.12612128499242914 1107 7 0.07109038798733991 1107 8 0.12306918470132899 1107 9 0.10640910502301892 1107 10-11 0.09384810081709105 1107 12-13 0.09040937488128975 1107 14-15 0.23945613078048922 1107 16-17 0.2865319604401435 1107 18-19 0.24199975136635032 1107 20-21 0.33602556952155993 1107 22-23 0.17860661849279325 1107 24-25 0.2106594368408352 1107 26-27 0.3891204014586762 1107 28-29 0.33179593149671405 1107 30-31 0.41879917731023397 1107 32-33 0.40424195966279797 1107 34-35 0.4947837678470606 1107 36-37 0.6434537932836903 1107 38-39 0.5836175827563608 1107 40-41 0.5217978443041176 1107 42-43 0.45633484198257435 1107 44-45 0.5246929561123395 1107 46-47 0.4482274644425175 1107 48-49 0.5683926102179697 1107 50-51 0.5186472589949318 1107 52-53 0.7428414083930903 1107 54-55 0.6033361935375581 1107 56-57 0.4397215019891192 1107 58-59 0.47304191114027105 1107 60-61 0.5824726565478144 1107 62-63 0.6560931533907066 1107 64-65 0.5250392976852041 1107 66-67 0.5680985119352542 1107 68-69 0.6051396245970793 1107 70-71 0.6615908702785163 1107 72-73 0.41912233021444223 1107 74-75 0.44111259019994264 1107 76-77 0.7259057790411276 1107 78-79 1.002044646193525 1107 80-81 0.8939606332477652 1107 82-83 0.7186316267425248 1107 84-85 0.6801017322607699 1107 86-87 0.8500288363028758 1107 88-89 0.8942232813851945 1107 90-91 1.1737412898964301 1107 92-93 1.0099580328088038 1107 94-95 1.0145316619903397 1107 96-97 0.9016028182515754 1107 98-99 1.24346580564211 1107 100-101 0.9056861044158602 1108 1 -0.04388663667480586 1108 2 -0.06726440255307864 1108 3 0.04856838894853155 1108 4 -0.04667246573023931 1108 5 0.006291552394039002 1108 6 -0.045430618481667295 1108 7 -0.0016472862377483466 1108 8 0.07009435632603811 1108 9 -0.012688044966573386 1108 10-11 -0.009209939540717471 1108 12-13 -0.0494439639995079 1108 14-15 -0.04838971902188405 1108 16-17 0.00405328351067169 1108 18-19 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-0.5511669361271956 2316 72-73 -0.6137572681967001 2316 74-75 -0.5822568526216259 2316 76-77 -0.5943245372623238 2316 78-79 -0.5794930043941378 2316 80-81 -0.796654952473375 2316 82-83 -0.5865848017146682 2316 84-85 -0.7209693944009601 2316 86-87 -0.7007678789195744 2316 88-89 -0.7254259811391606 2316 90-91 -0.9960731253942363 2316 92-93 -1.0496524057599181 2316 94-95 -1.0655808932101785 2316 96-97 -1.11705928114689 2316 98-99 -1.2680781605701164 2316 100-101 -0.43379911506433544 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 9.0 5 38.0 6 104.0 7 191.0 8 348.0 9 618.0 10 998.0 11 1696.0 12 2502.0 13 3545.0 14 4007.0 15 5419.0 16 7027.0 17 9405.0 18 9280.0 19 6426.0 20 5855.0 21 6142.0 22 6813.0 23 7531.0 24 8599.0 25 9782.0 26 11906.0 27 14176.0 28 16933.0 29 20658.0 30 25252.0 31 30888.0 32 37732.0 33 47783.0 34 64962.0 35 97005.0 36 136949.0 37 212596.0 38 192777.0 39 20878.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.60043356834561 17.997480616370133 13.960705427416361 20.4413803878679 2 12.944338671743758 23.548476629643517 40.705273972936254 22.80191072567647 3 23.093583681896312 28.440888750383458 29.74791470878963 18.717612858930597 4 11.445461052652082 16.557772183456933 40.1114103044793 31.88535645941169 5 11.787580283102933 41.59470606280464 33.732099120111805 12.88561453398063 6 26.361586817745792 37.10323469690846 19.773283925849817 16.76189455949593 7 25.390739505373304 36.59516864929614 20.35429255917455 17.659799286156 8 21.92026956619126 33.759562149712465 21.383084916271844 22.93708336782443 9 25.74824582333092 20.86080042071024 21.821714296844185 31.569239459114655 10-11 22.512721128516265 25.951832572906063 28.184372367517664 23.351073931060004 12-13 30.53085451898309 23.344500333549206 26.430049618487878 19.69459552897983 14-15 25.932573633460553 25.198454629828916 29.227344131187007 19.64162760552352 16-17 19.138989197801383 28.971064287300308 31.917857713268432 19.972088801629873 18-19 18.335321643691536 31.711862178441525 31.85867252284934 18.094143655017604 20-21 20.21517575852011 29.726440956921024 28.453354238996525 21.605029045562336 22-23 19.03660276480642 28.86383888355968 26.941767664717307 25.15779068691659 24-25 20.718041709739982 28.837704615495486 29.50358602092158 20.94066765384295 26-27 23.454557406744627 25.780628008385133 30.970839511402954 19.79397507346729 28-29 19.88522985679296 26.52008355772836 33.573553686814336 20.021132898664344 30-31 19.32666884163473 31.474190108440013 30.999868528049785 18.19927252187547 32-33 18.701884918219577 30.64289783655602 29.389142364644762 21.266074880579644 34-35 20.23915262292655 27.41845476006825 29.713077234727187 22.629315382278005 36-37 22.624496095639184 27.526121203944836 30.955081708363025 18.89430099205296 38-39 24.557904158432812 24.728635889504528 32.17664939221257 18.536810559850093 40-41 20.50514422081796 27.00329960672584 34.1382912663915 18.3532649060647 42-43 18.597195013335398 30.672535507901657 31.38466572878182 19.34560374998113 44-45 19.348070296062097 31.556097245060883 28.09203289546404 21.003799563412976 46-47 19.06838794623858 29.132049710128133 26.573064948243776 25.226497395389515 48-49 21.155102815934402 28.241392239259472 28.2270764046804 22.376428540125726 50-51 23.871800240544978 29.488281953770567 28.093900188443126 18.54601761724133 52-53 19.845301338579226 29.311330447442867 31.22867841474044 19.61468979923746 54-55 17.744233494183582 28.787341685859946 32.13953556316254 21.328889256793936 56-57 20.6721138254929 31.70207482214767 26.9671368817775 20.658674470581932 58-59 19.475335183044464 29.376126650351033 28.484301639906235 22.664236526698264 60-61 19.961239083275103 26.83151418352515 30.180730078422684 23.026516654777062 62-63 24.990516796834488 26.051126029944037 28.568861689368735 20.389495483852745 64-65 23.09012645653878 27.17797893527198 30.708195571829943 19.023699036359297 66-67 19.440611198488558 28.247917143455375 31.8777603022881 20.433711355767965 68-69 18.89792152079331 31.522862784339218 30.079881577569857 19.49933411729761 70-71 19.323942016000622 30.66563761461189 27.171648804335653 22.838771565051836 72-73 19.307094129047023 28.801949680328388 26.37858078464408 25.512375405980514 74-75 20.61202627491272 29.03752793778942 28.16771925382364 22.18272653347422 76-77 22.503081079366165 26.24347482300572 30.062451343350606 21.190992754277516 78-79 19.956955109632997 26.28226540778217 32.29024137276193 21.470538109822904 80-81 18.921100928152764 31.15332756473964 29.7470015567412 20.178569950366395 82-83 18.899725856637144 30.203830216149623 28.087375284247223 22.809068642966007 84-85 19.995909761548837 27.106156295802148 28.267297082783504 24.63063685986551 86-87 21.621744147105833 27.23112418172574 29.523925123121973 21.623206548046454 88-89 23.542932379589985 24.373580540273192 30.799178735485285 21.284308344651542 90-91 20.066996206415567 26.480265247892802 32.37791568878337 21.074822856908263 92-93 18.3487609986025 29.557085607716914 30.64737762152634 21.446775772154243 94-95 18.427838941991652 30.57092911714151 28.150871366870046 22.850360573996795 96-97 18.364635019258206 28.334445164023432 26.65793433219553 26.64298548452283 98-99 20.294838021688 27.004728120876287 27.873027312080325 24.827406545355387 100-101 22.317082256196105 27.40543875376217 28.61483520469949 21.662643785342233 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2800.0 1 1881.0 2 1846.0 3 2405.0 4 1591.5 5 1367.5 6 2424.0 7 4567.5 8 6025.5 9 5520.5 10 3883.0 11 2501.0 12 2122.0 13 2068.5 14 1912.5 15 1659.0 16 1467.5 17 1400.5 18 1452.0 19 1553.0 20 1736.5 21 1941.5 22 2246.0 23 2707.5 24 3200.5 25 3928.5 26 4966.5 27 6332.5 28 7832.5 29 9460.0 30 11361.5 31 13535.5 32 15916.5 33 18258.0 34 20964.0 35 23572.5 36 25587.0 37 26975.0 38 27676.5 39 27745.0 40 27341.0 41 26918.0 42 26682.5 43 27139.5 44 29880.5 45 31754.0 46 36093.5 47 50980.5 48 127985.5 49 126381.0 50 53144.0 51 40421.0 52 26580.5 53 18254.0 54 14639.0 55 12377.5 56 10338.0 57 8420.0 58 6721.0 59 5260.0 60 4053.0 61 3109.0 62 2240.0 63 1444.5 64 871.0 65 599.0 66 448.5 67 344.5 68 293.0 69 233.5 70 164.5 71 129.0 72 109.0 73 134.5 74 126.5 75 67.0 76 43.0 77 38.5 78 30.5 79 18.5 80 10.5 81 6.0 82 6.0 83 5.5 84 2.0 85 0.5 86 1.0 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 1.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27024789766612944 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.002532052374529501 16-17 6.817064085271733E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.86933148947981E-5 26-27 0.0017042660213179334 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0041876250809526365 36-37 0.022058071647343535 38-39 0.007596157123588502 40-41 0.019233859383445247 42-43 0.0055023445831121846 44-45 6.330130936323753E-4 46-47 1.947732595791924E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 7.790930383167696E-4 60-61 0.002678132319213895 62-63 0.0018990392808971256 64-65 0.0 66-67 0.0 68-69 2.4346657447399045E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.2373799101121407 78-79 0.0 80-81 0.00102255961279076 82-83 0.0 84-85 0.0 86-87 0.10951126519840092 88-89 0.3679753806599892 90-91 0.36929010016214875 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 4.86933148947981E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1026835.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.801021590495296 #Duplication Level Percentage of deduplicated Percentage of total 1 77.90350997030254 37.23867362065797 2 13.878573079181237 13.26819942806418 3 4.298910679727127 6.164769666517418 4 1.6802055579530153 3.2126216860872914 5 0.8210464365271132 1.9623429219615882 6 0.43095272106859195 1.2359988194569478 7 0.2409569759711184 0.8062592727543122 8 0.14705205497573542 0.5623390763857465 9 0.1047500301882359 0.4506442609169608 >10 0.4145209278970737 3.4800661328042515 >50 0.03107331386968945 1.0472522090189724 >100 0.03168556589106588 3.297729187647459 >500 0.004701729125499943 1.4936911321157762 >1k 0.009199035245543366 9.001331022969316 >5k 0.0016353840436521539 5.2411106608767355 >10k+ 0.0012265380327391153 11.536970901765095 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 33731 3.284948409432869 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 30189 2.9400049667181194 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 19027 1.8529754050066467 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 12843 1.250736486387784 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 11770 1.146240632623547 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 10506 1.0231439325694975 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 9254 0.9012158720729231 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 8067 0.7856179425126725 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7688 0.7487084098224155 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 6831 0.6652480680927315 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5881 0.5727307697926152 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 5466 0.5323153184299327 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 5430 0.5288093997575073 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 5019 0.48878349491398326 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4910 0.4781683522669173 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 4755 0.4630734246495299 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 4301 0.41885989472505325 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 4064 0.3957792634649189 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 4042 0.3936367576095478 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3778 0.36792668734509437 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 3523 0.3430930967487474 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3354 0.3266347563143056 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3130 0.304820151241436 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2673 0.2603144614275906 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 2622 0.25534774330832116 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 2614 0.25456865027000447 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2459 0.23947372265261702 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 2457 0.23927894939303782 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 2188 0.21308194597963645 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 2115 0.20597272200499595 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 2018 0.19652621891540512 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 2000 0.1947732595791924 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 1873 0.18240515759591366 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1833 0.17850969240432982 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 1701 0.1656546572721031 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 1563 0.15221530236113884 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1548 0.1507545029142949 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 1494 0.1454956249056567 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1488 0.14491130512691913 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1455 0.14169754634386245 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1389 0.1352700287777491 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1387 0.13507525551816993 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1363 0.1327379764032196 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1350 0.13147195021595484 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 1237 0.12046726104973048 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1236 0.12036987441994089 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 1183 0.1152083830410923 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1155 0.11248155740698358 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 1148 0.11179985099845642 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1142 0.11121553121971886 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1117 0.10878086547497894 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 1112 0.10829393232603096 No Hit GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1087 0.10585926658129105 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 1059 0.10313244094718235 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1056 0.10284028105781358 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 1053 0.10254812116844479 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 1040 0.10128209498118004 No Hit TCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTA 1033 0.10060038857265287 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 9.738662978959618E-5 0.0 18-19 0.0 0.0 0.0 9.738662978959618E-5 0.0 20-21 0.0 0.0 0.0 2.4346657447399048E-4 0.0 22-23 0.0 0.0 0.0 9.738662978959618E-4 0.0 24-25 0.0 0.0 0.0 0.002726825634108693 0.0 26-27 0.0 0.0 0.0 0.004918024804374607 0.0 28-29 0.0 0.0 0.0 0.009543889719380427 0.0 30-31 0.0 0.0 0.0 0.021814605072869545 0.0 32-33 0.0 0.0 0.0 0.04489523633300384 0.0 34-35 0.0 0.0 0.0 0.0942215643214343 0.0 36-37 0.0 0.0 0.0 0.16662852356999908 0.0 38-39 0.0 0.0 0.0 0.2427361747505685 0.0 40-41 0.0 0.0 0.0 0.3246383304036189 0.0 42-43 0.0 0.0 0.0 0.4038623537374554 0.0 44-45 0.0 0.0 0.0 0.4905851475650908 0.0 46-47 0.0 0.0 0.0 0.609445529223293 0.0 48-49 0.0 0.0 0.0 0.7499257426947854 0.0 50-51 0.0 0.0 0.0 0.8874843572725901 0.0 52-53 0.0 0.0 0.0 1.0172033481523322 0.0 54-55 0.0 0.0 0.0 1.1532037766535033 0.0 56-57 0.0 0.0 0.0 1.3014262271932686 0.0 58-59 0.0 0.0 0.0 1.5307230470328728 0.0 60-61 0.0 0.0 0.0 1.8772246758242561 0.0 62-63 0.0 0.0 0.0 2.1660734197801985 0.0 64-65 0.0 0.0 0.0 2.3856802699557376 0.0 66-67 0.0 0.0 0.0 2.631581510174468 0.0 68-69 0.0 0.0 0.0 2.910009884742924 0.0 70-71 0.0 0.0 0.0 3.253784687900198 0.0 72-73 0.0 0.0 0.0 3.6519012304800675 0.0 74-75 0.0 0.0 0.0 4.026644981910433 0.0 76-77 0.0 0.0 0.0 4.379914981472194 0.0 78-79 0.0 0.0 0.0 4.778956697035064 0.0 80-81 0.0 0.0 0.0 5.216904371198878 0.0 82-83 0.0 0.0 0.0 5.681487288610146 0.0 84-85 0.0 0.0 0.0 6.380577210554764 0.0 86-87 0.0 0.0 0.0 7.181630933889086 0.0 88-89 0.0 0.0 0.0 7.766778498979875 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTAACG 30 0.003971751 31.633894 14-15 GTGTAGC 490 0.0 27.191566 1 CCATCCG 90 9.0037024E-4 26.360296 9 GGACCCG 110 9.545761E-5 25.881016 9 AGTACTC 575 0.0 25.580946 5 CGCAGCG 75 1.0708645E-6 25.305882 94-95 GTAGCCC 475 0.0 22.975077 3 GGTCGGG 105 0.002173295 22.65964 1 TAGCCCT 840 0.0 22.594538 4 GTATAGC 620 0.0 22.257614 1 GTACTAA 220 5.5319106E-8 21.629656 1 GTAAGCG 55 0.0028962917 21.567513 94-95 AAGTACT 685 0.0 21.473057 4 TACCTGG 660 0.0 20.848597 2 CGCAGAT 80 5.033594E-5 20.758732 94-95 AAAGTAC 650 0.0 20.439367 3 GTAGGAC 585 0.0 20.27715 3 CATGGGG 1605 0.0 20.102804 4 AGTACTT 5150 0.0 20.039917 12-13 GCGTTGT 95 8.334158E-6 19.978329 94-95 >>END_MODULE