FastQCFastQC Report
Fri 27 May 2016
SRR939283_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939283_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2115748
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41320.1952973605552268No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34130.16131410735115903No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22110.10450204844811387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48400.034.417751
GTATCAA71100.031.372491
ATCAACG84450.025.6618143
TATCAAC85800.025.479122
TCAACGC85950.025.2139664
CAACGCA85850.025.1328625
AACGCAG88900.024.4671576
GTACATG63250.022.9605161
TACATGG64550.021.7453442
ACGCAGA100800.021.3906237
CGCAGAG101100.021.1865318
ACATGGG64950.020.8082963
CATGGGG41300.019.5195474
GAGTACT52800.019.51896712-13
GCAGAGT108550.019.2076789
GTACTTT54800.018.68109514-15
AGAGTAC87700.018.23434610-11
AGTACTT54550.017.85042412-13
TAACCCG1350.00949346417.5633375
CAGAGTA106300.016.9158810-11
Produced by FastQC (version 0.11.5)