##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939283_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2115748 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.52934564986 33.0 31.0 34.0 30.0 34.0 2 31.612141427050858 33.0 31.0 34.0 28.0 34.0 3 31.6567081712945 34.0 31.0 34.0 28.0 34.0 4 35.14125784356171 37.0 35.0 37.0 33.0 37.0 5 35.16590397344107 37.0 35.0 37.0 33.0 37.0 6 35.23703957182046 37.0 35.0 37.0 33.0 37.0 7 35.159175856481966 37.0 35.0 37.0 32.0 37.0 8 35.13426835331996 37.0 35.0 37.0 32.0 37.0 9 36.706588875423726 39.0 37.0 39.0 32.0 39.0 10-11 36.58521761570849 39.0 37.0 39.0 32.0 39.0 12-13 36.46591961802635 39.0 37.0 39.0 32.0 39.0 14-15 37.635890474669004 40.0 38.0 41.0 32.0 41.0 16-17 37.5351925182016 40.0 37.5 41.0 32.0 41.0 18-19 37.42760172761595 40.0 37.0 41.0 32.0 41.0 20-21 37.39001809289197 40.0 37.0 41.0 31.5 41.0 22-23 37.397775396691856 40.0 37.0 41.0 31.5 41.0 24-25 37.30703467520706 40.0 37.0 41.0 31.0 41.0 26-27 37.20590117537627 40.0 37.0 41.0 31.0 41.0 28-29 37.04306420235302 40.0 37.0 41.0 30.5 41.0 30-31 36.861256160941664 40.0 36.5 41.0 30.0 41.0 32-33 36.645546634098196 40.0 36.0 41.0 30.0 41.0 34-35 36.34806295456737 39.0 36.0 41.0 29.5 41.0 36-37 36.095699015194626 39.0 35.0 41.0 28.5 41.0 38-39 35.49045207652329 38.5 34.5 40.5 26.0 41.0 40-41 35.46764217666754 38.5 35.0 40.0 26.5 41.0 42-43 35.66849076544088 39.0 35.0 41.0 27.0 41.0 44-45 35.588328808534854 39.0 35.0 41.0 26.0 41.0 46-47 35.343837734928734 39.0 35.0 41.0 25.5 41.0 48-49 35.115507612437774 39.0 35.0 41.0 24.0 41.0 50-51 34.01649558454031 38.0 33.5 40.0 23.5 40.5 52-53 34.14180209552366 38.0 33.5 39.5 23.0 40.5 54-55 34.62162318007627 38.5 34.0 40.0 23.0 41.0 56-57 34.530391615636645 38.0 34.0 40.0 23.0 41.0 58-59 34.30538561303142 38.0 34.0 40.0 22.5 41.0 60-61 33.99040693882258 38.0 34.0 40.0 22.0 41.0 62-63 33.58918878807873 37.5 33.0 40.0 20.5 41.0 64-65 33.18691852715919 37.0 33.0 40.0 18.5 41.0 66-67 32.813076982703045 36.0 32.0 39.0 17.5 41.0 68-69 32.39032342226012 36.0 32.0 39.0 14.0 40.5 70-71 31.9621691713758 35.0 31.0 39.0 12.0 40.0 72-73 31.49643530325918 35.0 31.0 38.0 8.0 40.0 74-75 31.018528671656547 35.0 31.0 37.0 7.0 39.0 76-77 30.524153396340207 35.0 30.0 36.5 3.5 39.0 78-79 30.10086338259566 34.0 30.0 36.0 2.0 39.0 80-81 29.660835316871385 34.0 29.5 36.0 2.0 37.5 82-83 29.208448974074418 34.0 29.0 35.0 2.0 37.0 84-85 28.762228299400498 34.0 29.0 35.0 2.0 36.5 86-87 28.304808512166854 34.0 28.5 35.0 2.0 36.0 88-89 27.844157716325384 34.0 27.0 35.0 2.0 36.0 90-91 27.40560926915682 33.5 26.0 35.0 2.0 35.0 92-93 26.91478309326063 33.0 25.5 35.0 2.0 35.0 94-95 26.381908195115862 33.0 25.0 35.0 2.0 35.0 96-97 25.716253306159334 32.5 22.5 35.0 2.0 35.0 98-99 24.84378432592161 32.0 10.0 34.5 2.0 35.0 100-101 22.086962152392438 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.43764319796778395 1101 2 0.47521280394981247 1101 3 0.5536768649962447 1101 4 0.4269352172059442 1101 5 0.28495291268570355 1101 6 0.5159975484612218 1101 7 0.4909823945609233 1101 8 0.4234504199755875 1101 9 0.541521330291971 1101 10-11 0.641445070621586 1101 12-13 0.6798123002381828 1101 14-15 0.7257186875438748 1101 16-17 0.7475231977655383 1101 18-19 0.8201595854005603 1101 20-21 0.861020510969162 1101 22-23 0.8903525670921724 1101 24-25 0.81271848659037 1101 26-27 0.9316656439663262 1101 28-29 0.9454807764206308 1101 30-31 1.0216651256594105 1101 32-33 0.9987251658163032 1101 34-35 0.8444508810048106 1101 36-37 0.8793013580358959 1101 38-39 1.268092815279367 1101 40-41 1.066388873438349 1101 42-43 1.0537264334062044 1101 44-45 0.9186629041618062 1101 46-47 1.1468371278100165 1101 48-49 0.9629504242973965 1101 50-51 0.8157127934666519 1101 52-53 1.006923798224534 1101 54-55 1.051953189067774 1101 56-57 1.0234122337511806 1101 58-59 0.960333881309495 1101 60-61 1.0849436480912047 1101 62-63 1.1207189211511306 1101 64-65 1.0008463276814084 1101 66-67 0.8791541425830047 1101 68-69 0.9262231350831627 1101 70-71 1.1028759800010022 1101 72-73 1.1017512835125594 1101 74-75 0.9787849823869941 1101 76-77 0.9650242815180157 1101 78-79 1.0457312762550117 1101 80-81 0.9144567935455257 1101 82-83 1.0765864314002584 1101 84-85 1.1586132517662868 1101 86-87 0.9225525795604632 1101 88-89 0.8858982213942745 1101 90-91 1.0564831600606936 1101 92-93 1.0220365159367262 1101 94-95 1.0365072169635248 1101 96-97 1.0318182077057436 1101 98-99 1.183548775492163 1101 100-101 0.5992479270178741 1102 1 0.4779810114065093 1102 2 0.4871104797711361 1102 3 0.4787134717033048 1102 4 0.48910908021551336 1102 5 0.49783827516154133 1102 6 0.4227635393422844 1102 7 0.5325334872066421 1102 8 0.5893909048110828 1102 9 0.6198924211123185 1102 10-11 0.7481838792865716 1102 12-13 0.66518995447246 1102 14-15 0.8991258493633794 1102 16-17 0.782900256768805 1102 18-19 0.8386385320797558 1102 20-21 0.6542218815877661 1102 22-23 0.7388246090916724 1102 24-25 0.7500679427476413 1102 26-27 0.7354035954986458 1102 28-29 0.7630856263628942 1102 30-31 0.8153754187908362 1102 32-33 0.7460967248850423 1102 34-35 0.7933374021970181 1102 36-37 0.7560081829546519 1102 38-39 1.1833606890745259 1102 40-41 1.0940730676741381 1102 42-43 0.9139989301621938 1102 44-45 0.9581296962238071 1102 46-47 1.0258460086566785 1102 48-49 0.8862325876049724 1102 50-51 0.8128909024574185 1102 52-53 0.9631604303281165 1102 54-55 0.9793240933824023 1102 56-57 0.860771808835608 1102 58-59 0.9881584508582719 1102 60-61 0.9543683176387887 1102 62-63 0.9536956119048128 1102 64-65 0.8028293397884099 1102 66-67 0.8851892889899915 1102 68-69 0.8139888561261728 1102 70-71 0.7461704050673745 1102 72-73 0.7379788284227438 1102 74-75 0.6148423904337186 1102 76-77 0.7415031329602613 1102 78-79 0.6004632635726246 1102 80-81 0.7079190737564787 1102 82-83 0.6024348339986396 1102 84-85 0.5136358110215333 1102 86-87 0.5048581473513245 1102 88-89 0.438485392975565 1102 90-91 0.4202040284630506 1102 92-93 0.4929723778113839 1102 94-95 0.6230379328677031 1102 96-97 0.605187373945828 1102 98-99 0.6305439501527808 1102 100-101 0.27391003421501026 1103 1 0.5302923179968708 1103 2 0.48783911100424504 1103 3 0.549312686253554 1103 4 0.5868958076456039 1103 5 0.5317762988192456 1103 6 0.49734593796686255 1103 7 0.5369567939854178 1103 8 0.562340286284126 1103 9 0.7019672095884602 1103 10-11 0.6966380034865409 1103 12-13 0.6290299735493505 1103 14-15 0.956902626193191 1103 16-17 0.9038221298368327 1103 18-19 0.9329484373429864 1103 20-21 0.9540845456401925 1103 22-23 0.9417638633265639 1103 24-25 1.022596631682454 1103 26-27 0.9966730919040643 1103 28-29 1.045065177584931 1103 30-31 1.0005184071504019 1103 32-33 1.004433296579414 1103 34-35 0.960078403969284 1103 36-37 0.8722800639713739 1103 38-39 1.3646802034381267 1103 40-41 1.3275404985940114 1103 42-43 1.0065476086164935 1103 44-45 1.0608696440567584 1103 46-47 1.0489546586133969 1103 48-49 0.986508437085952 1103 50-51 1.0237641412306075 1103 52-53 1.1634773481149665 1103 54-55 1.2289487378986763 1103 56-57 1.0963898058798662 1103 58-59 1.2380053647002 1103 60-61 1.1452807405805174 1103 62-63 1.1114221941385694 1103 64-65 1.1554169374544259 1103 66-67 1.2243435762856052 1103 68-69 1.1471661593368623 1103 70-71 0.9721873976309183 1103 72-73 1.0910580681733286 1103 74-75 1.098038976612397 1103 76-77 1.1586452499063107 1103 78-79 1.3410837924264207 1103 80-81 1.3116320018825256 1103 82-83 1.2725915706727875 1103 84-85 1.2205457374812987 1103 86-87 1.0760255470651678 1103 88-89 1.0664867648630576 1103 90-91 1.162571800294021 1103 92-93 0.8962609334434823 1103 94-95 0.931113522102212 1103 96-97 0.9206640901299785 1103 98-99 0.9120318592902592 1103 100-101 0.6647897791499169 1104 1 0.2573390026816327 1104 2 0.19400477690507145 1104 3 0.1888018191195293 1104 4 0.21039519770289417 1104 5 0.2228174449272231 1104 6 0.16793855706134764 1104 7 0.19385599562380662 1104 8 0.14810129698339125 1104 9 0.34009157468048556 1104 10-11 0.37000769390769506 1104 12-13 0.30833948104036324 1104 14-15 0.2515756015881081 1104 16-17 0.3891317781391166 1104 18-19 0.45926673500173365 1104 20-21 0.42500629911413057 1104 22-23 0.4558751645807888 1104 24-25 0.5687535136418944 1104 26-27 0.37949655910869495 1104 28-29 0.5987208585774724 1104 30-31 0.4503723513747673 1104 32-33 0.33490777788863824 1104 34-35 0.46888389809952 1104 36-37 0.3945997285531746 1104 38-39 0.9252975411375672 1104 40-41 0.6017247151828187 1104 42-43 0.4759482171702487 1104 44-45 0.42525389886576903 1104 46-47 0.46384968955595696 1104 48-49 0.35560412217829906 1104 50-51 0.3485524094483097 1104 52-53 0.3977404908597606 1104 54-55 0.45821822912455445 1104 56-57 0.389503799311548 1104 58-59 0.41474949672561934 1104 60-61 0.4606189678240469 1104 62-63 0.5166518961215729 1104 64-65 0.3948653116828993 1104 66-67 0.39149146787625 1104 68-69 0.28777435867198164 1104 70-71 0.567126884704301 1104 72-73 0.3583879128355001 1104 74-75 0.45646880637923104 1104 76-77 0.5057812012756528 1104 78-79 0.3766799485660073 1104 80-81 0.34282169909518245 1104 82-83 0.430561824051658 1104 84-85 0.4707130107380273 1104 86-87 0.4963741411795475 1104 88-89 0.4123672687758386 1104 90-91 0.5698927173298394 1104 92-93 0.5355548942203612 1104 94-95 0.4624868966060838 1104 96-97 0.36064771483249203 1104 98-99 0.3396976106836469 1104 100-101 0.26251890998514327 1105 1 0.22568333080753789 1105 2 0.17117265414216476 1105 3 0.26467639649383656 1105 4 0.22175190267869027 1105 5 0.2906807552085624 1105 6 0.3487721196212661 1105 7 0.26486611923987624 1105 8 0.3864163418459796 1105 9 0.34810030122690705 1105 10-11 0.3808509775225275 1105 12-13 0.4661985347514488 1105 14-15 0.4649451354001215 1105 16-17 0.47936436979453134 1105 18-19 0.4381606826576956 1105 20-21 0.6449626617628326 1105 22-23 0.6322454487778728 1105 24-25 0.503805134083315 1105 26-27 0.38001125074292474 1105 28-29 0.5425604604784482 1105 30-31 0.5887433344531132 1105 32-33 0.5305196997035395 1105 34-35 0.35060586669050764 1105 36-37 0.21451435695089316 1105 38-39 0.9202329615991758 1105 40-41 0.645426619461027 1105 42-43 0.4114403914785356 1105 44-45 0.2642727444090909 1105 46-47 0.34341854494966384 1105 48-49 0.35428084386628456 1105 50-51 0.3887356567610496 1105 52-53 0.42098695366834704 1105 54-55 0.5097071395234067 1105 56-57 0.40065541289349227 1105 58-59 0.5815250604555757 1105 60-61 0.728363357431185 1105 62-63 0.6391415763060238 1105 64-65 0.5661394904685508 1105 66-67 0.3887167291310476 1105 68-69 0.35495102850971705 1105 70-71 0.3517962799179628 1105 72-73 0.39069153955808034 1105 74-75 0.410303033869468 1105 76-77 0.3541796291854524 1105 78-79 0.3445256366926728 1105 80-81 0.4811918738178065 1105 82-83 0.4516979010366846 1105 84-85 0.4345659990097097 1105 86-87 0.49105573794538415 1105 88-89 0.38667968717521717 1105 90-91 0.43474464850319805 1105 92-93 0.5447795032475682 1105 94-95 0.5218952679840996 1105 96-97 0.47319531254941793 1105 98-99 0.3578613636898602 1105 100-101 0.195992966501219 1106 1 0.1686832143280519 1106 2 0.137811192015036 1106 3 0.17935305678865276 1106 4 0.08676487243567266 1106 5 0.14640164630559127 1106 6 0.06457620335197589 1106 7 0.061179576896684296 1106 8 0.021164511191557267 1106 9 0.06521127143421523 1106 10-11 0.1649986761840836 1106 12-13 0.29206994273454256 1106 14-15 0.4483625099079518 1106 16-17 0.37972797287253 1106 18-19 0.43114351741205326 1106 20-21 0.44774908270455427 1106 22-23 0.4856119175184972 1106 24-25 0.40859963464800586 1106 26-27 0.3560826226017966 1106 28-29 0.44021574845783107 1106 30-31 0.2310260339531851 1106 32-33 0.23239034244091528 1106 34-35 0.29727351775360233 1106 36-37 0.1586170094538275 1106 38-39 0.6144392318480669 1106 40-41 0.40879976739821444 1106 42-43 0.48865058973642306 1106 44-45 0.33372280310539537 1106 46-47 0.20226489469858677 1106 48-49 0.3011008313898387 1106 50-51 0.274563377141682 1106 52-53 0.28663900160266564 1106 54-55 0.24747918760916576 1106 56-57 0.3917468479541739 1106 58-59 0.471344263357274 1106 60-61 0.5729213712309971 1106 62-63 0.3839364688475655 1106 64-65 0.3404510198664852 1106 66-67 0.22165363132457827 1106 68-69 0.49749174232184146 1106 70-71 0.28456590153334105 1106 72-73 0.28839994485591447 1106 74-75 0.45890426668122686 1106 76-77 0.5108531131314358 1106 78-79 0.6926077674254145 1106 80-81 0.671971241848663 1106 82-83 0.4217529849208468 1106 84-85 0.46853402305214686 1106 86-87 0.6064991584245263 1106 88-89 0.5563709229958462 1106 90-91 0.48791196627460565 1106 92-93 0.42484360482659156 1106 94-95 0.5412815302197345 1106 96-97 0.46079180622734484 1106 98-99 0.4998613276101729 1106 100-101 0.5429905908096622 1107 1 0.2289991420437083 1107 2 0.17559373579038962 1107 3 0.27591045464978947 1107 4 0.23346737336495949 1107 5 0.29994893562087555 1107 6 0.30986277619541625 1107 7 0.3859802792970086 1107 8 0.2554516900556152 1107 9 0.3439047580517851 1107 10-11 0.39294234566396824 1107 12-13 0.3742062056974049 1107 14-15 0.4946017959403193 1107 16-17 0.566753273961119 1107 18-19 0.5522752364179553 1107 20-21 0.3829865358675235 1107 22-23 0.4312075761200589 1107 24-25 0.5928816199828688 1107 26-27 0.470569563978934 1107 28-29 0.4711462089529306 1107 30-31 0.4680700295692759 1107 32-33 0.5340330219709841 1107 34-35 0.5199582217540168 1107 36-37 0.6407484381882682 1107 38-39 1.0787940920876906 1107 40-41 0.9341755469992137 1107 42-43 0.5771396168000464 1107 44-45 0.575984390173879 1107 46-47 0.7295387622315843 1107 48-49 0.695111353022682 1107 50-51 0.6865684430646652 1107 52-53 0.8019956250291216 1107 54-55 0.7129153253829372 1107 56-57 0.49823575437984147 1107 58-59 0.5753220929759806 1107 60-61 0.5817030084796428 1107 62-63 0.6307521386288357 1107 64-65 0.5150603020641356 1107 66-67 0.44804653694193064 1107 68-69 0.7290474732452026 1107 70-71 0.6907298258641674 1107 72-73 0.5780493827244264 1107 74-75 0.6197758475806232 1107 76-77 0.6837757504512858 1107 78-79 0.7040807851358934 1107 80-81 0.5976528190614161 1107 82-83 0.6622651039177754 1107 84-85 0.5787305866996633 1107 86-87 0.8244213852704796 1107 88-89 0.9020502660111376 1107 90-91 0.8241575955985994 1107 92-93 0.7665576828619223 1107 94-95 0.6557645519522026 1107 96-97 0.6359783649818382 1107 98-99 0.9255308192518577 1107 100-101 0.9610458460405944 1108 1 0.019947537070780896 1108 2 0.0693862951129276 1108 3 0.12558486652833167 1108 4 0.040455927804735836 1108 5 0.09042649322083918 1108 6 0.006667291471053716 1108 7 0.05766304460560434 1108 8 0.18517548244480508 1108 9 0.15015941007018085 1108 10-11 0.18984138995330113 1108 12-13 0.2352557861119351 1108 14-15 0.14720171597876686 1108 16-17 0.26710661469515884 1108 18-19 0.17994491014930958 1108 20-21 0.2680909324768095 1108 22-23 0.21530788107980214 1108 24-25 0.24395036139657833 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2316 54-55 -1.7105950798033547 2316 56-57 -1.5367469921127466 2316 58-59 -1.4288929488135622 2316 60-61 -1.4331807193777877 2316 62-63 -1.6802839577861235 2316 64-65 -1.7100456515797653 2316 66-67 -2.0588238379686103 2316 68-69 -1.9443659334709125 2316 70-71 -1.6129088006069452 2316 72-73 -1.34676601467525 2316 74-75 -1.3287810710452952 2316 76-77 -1.2490647428203019 2316 78-79 -1.1696768515842138 2316 80-81 -1.1648748770568886 2316 82-83 -1.189109753926001 2316 84-85 -1.1789859471096484 2316 86-87 -1.1659559082277973 2316 88-89 -1.2463109944801651 2316 90-91 -1.1849189216827867 2316 92-93 -1.3847939182620372 2316 94-95 -1.171768253770555 2316 96-97 -0.996457770690764 2316 98-99 -1.343623037124722 2316 100-101 -0.7412334843491806 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12197.0 3 3567.0 4 3573.0 5 3824.0 6 4195.0 7 4896.0 8 5818.0 9 7115.0 10 7811.0 11 9133.0 12 10754.0 13 12947.0 14 12979.0 15 13144.0 16 15088.0 17 17592.0 18 16564.0 19 14103.0 20 14578.0 21 15410.0 22 16776.0 23 18540.0 24 21300.0 25 23827.0 26 27355.0 27 31397.0 28 35884.0 29 42660.0 30 50673.0 31 61047.0 32 75492.0 33 96383.0 34 128099.0 35 180836.0 36 276226.0 37 416433.0 38 361355.0 39 46174.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.680334567767794 19.039871664324846 15.12789006517283 23.15190370273453 2 15.068162985060493 19.93015602433885 40.37805715368042 24.623623836920235 3 18.966960659063208 23.820392206628703 29.809749385135092 27.402897749173 4 12.935004336012298 16.798957273110044 37.39868626642108 32.86735212445658 5 14.174552075912143 36.87946218793106 34.30835139682588 14.63763433933092 6 31.985123462485713 35.937200570645814 17.757683726894406 14.31999223997407 7 28.705651514025732 32.13469511961378 20.76153012126309 18.398123245097395 8 26.02987408732003 35.28678953431904 19.871834315157518 18.811502063203413 9 25.917288432109746 17.217195137709822 20.43899961517835 36.42651681500208 10-11 24.916065931902423 26.617606135066886 27.558533778078985 20.90779415495171 12-13 25.61615610960154 23.710688431001213 27.941393595061854 22.731761864335397 14-15 22.576383220858844 25.434253599227013 26.004715432878676 25.98464774703546 16-17 22.435137164051522 28.57589335672732 26.609241618869632 22.37972786035152 18-19 22.295399866429566 27.35377658333266 28.609892447874635 21.740931102363138 20-21 23.026297879774425 26.554483715947015 28.550573583192346 21.868644821086217 22-23 22.650184399291994 26.359570452271253 28.450518043764855 22.539727104671897 24-25 22.627366323401933 26.59528597620186 28.462535319187428 22.314812381208785 26-27 22.456070575897144 26.754377554535218 28.391481745837282 22.398070123730353 28-29 22.249159661577803 26.934624878467854 28.51289158089679 22.30332387905755 30-31 22.526483825657674 26.59827289998187 28.45063761434664 22.424605660013814 32-33 22.381750618521387 26.690627095817494 28.34611725285836 22.581505032802763 34-35 22.841486715454774 26.846891418314705 28.041011040886758 22.27061082534376 36-37 22.821168834807917 26.553702636866934 28.189983980562005 22.435144547763144 38-39 22.45670223667822 26.81936775378617 28.28526875484703 22.438661254688576 40-41 22.714205148556964 26.720315665748252 28.168715215339713 22.396763970355067 42-43 22.61037158240835 26.662301430627767 28.05050347014898 22.676823516814903 44-45 22.644645680377394 26.849698981044245 27.89605354972541 22.609601788852956 46-47 22.659152420155788 26.984664032596555 27.591611339085357 22.764572208162292 48-49 22.78524086779704 26.937413340571233 27.629026524463875 22.648319267167857 50-51 22.66203141969912 27.093091341845586 27.53424078558271 22.71063645287259 52-53 22.84450373623785 27.11705105542664 27.33914990259281 22.6992953057427 54-55 22.561488662382395 26.892784323631837 27.632030039914568 22.9136969740712 56-57 22.645643383565748 27.205719365846043 27.487211690487072 22.66142556010114 58-59 22.75688546250489 26.954369335677214 27.558745695399438 22.72999950641846 60-61 22.727426901736074 26.938192406409577 27.79549729921919 22.538883392635157 62-63 22.552644738897957 26.978098667835575 27.7352207443613 22.734035848905172 64-65 22.475892871983493 26.805939103530648 27.701696106529646 23.01647191795622 66-67 22.511112002957976 27.062491525146005 27.465556174835022 22.960840297060997 68-69 22.43305821868112 26.856893276240967 27.701451162300227 23.008597342777687 70-71 22.47173240037351 26.85103737317221 27.613025276738924 23.064204949715354 72-73 22.605959834001563 26.74749069369603 27.61174926219223 23.034800210110177 74-75 22.446707077423607 27.084025481337235 27.32955348290459 23.13971395833457 76-77 22.515189336374803 26.865330753720144 27.426465473298794 23.19301443660626 78-79 22.525308034596254 26.919535345790425 27.4449359599012 23.110220659712123 80-81 22.486655242489203 27.044702652904796 27.398845824165623 23.06979628044037 82-83 22.581072492745456 26.959700507123735 27.394913853539745 23.06431314659107 84-85 22.50731405977978 26.843495620823187 27.374538026582034 23.274652292815 86-87 22.435536596153742 26.76991985595582 27.528910568534354 23.265632979356088 88-89 22.441755332030937 26.652580563361823 27.50083338952579 23.404830715081452 90-91 22.422245727391857 26.740426142287127 27.61983559785642 23.217492532464604 92-93 22.56076186857292 26.437884437266963 27.684423017550525 23.31693067660959 94-95 22.37539630707119 26.48079320786083 27.638354212432464 23.505456272635517 96-97 22.382140359334908 26.421766704228496 27.671186356150262 23.524906580286334 98-99 22.347083926031296 26.44127125104192 27.42256534717654 23.789079475750246 100-101 22.544427209702697 25.843224843100664 27.950553887349688 23.66179405984695 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2242.0 1 1291.5 2 323.5 3 303.5 4 274.5 5 270.5 6 372.5 7 652.0 8 1016.0 9 1251.0 10 1348.0 11 1386.5 12 1535.5 13 1759.0 14 1942.0 15 2089.0 16 2228.5 17 2411.5 18 2558.5 19 2643.0 20 2743.5 21 3101.0 22 3652.5 23 4385.0 24 5319.5 25 6776.5 26 9128.0 27 12047.5 28 14917.5 29 18314.0 30 22378.0 31 26647.0 32 30516.0 33 34187.0 34 38383.0 35 42265.0 36 46743.5 37 51837.0 38 55851.5 39 58561.0 40 61455.0 41 66468.5 42 71787.0 43 75756.0 44 79070.5 45 82677.0 46 84715.5 47 86049.0 48 89825.0 49 90204.0 50 86467.5 51 83172.0 52 79794.0 53 76473.5 54 73324.5 55 67683.0 56 59270.0 57 51931.0 58 45246.5 59 38132.5 60 31878.0 61 26444.0 62 21587.5 63 16953.0 64 13082.0 65 10075.0 66 7717.5 67 6200.5 68 4709.5 69 3213.5 70 2339.5 71 1822.5 72 1527.5 73 1225.5 74 908.0 75 712.5 76 489.0 77 274.0 78 173.5 79 109.5 80 67.5 81 52.0 82 32.5 83 25.0 84 18.5 85 13.5 86 11.0 87 8.0 88 9.5 89 8.5 90 5.5 91 3.5 92 4.5 93 5.5 94 3.0 95 1.5 96 1.5 97 1.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2968217386947784 2 0.18423744226628125 3 0.1844265006985709 4 0.20612095580381026 5 0.22100930734662164 6 0.11121362279439706 7 0.12945776151035002 8 0.14245552873026465 9 0.1456222574711166 10-11 0.1313956104413191 12-13 0.12128098431382188 14-15 0.1369964664979005 16-17 0.1127024579486782 18-19 0.14203014725761293 20-21 0.16748213870460943 22-23 0.17107424891811313 24-25 0.19437570069781468 26-27 0.1746663591316168 28-29 0.1726339809845029 30-31 0.16169222421573837 32-33 0.17272851020064772 34-35 0.3584547876212101 36-37 0.4812009747852769 38-39 0.4458234156430728 40-41 0.4355906279953945 42-43 0.4591520351194944 44-45 0.46402028975095333 46-47 0.4670452246675881 48-49 0.4803738441440096 50-51 0.47259881611609705 52-53 0.47992483036732164 54-55 0.4608299287060652 56-57 0.4225928607754799 58-59 0.41120209023002735 60-61 0.3661352864329778 62-63 0.4762854555457456 64-65 0.47467845887128335 66-67 0.48467492347859953 68-69 0.4964910754967038 70-71 0.4884324598203567 72-73 0.481437297825639 74-75 0.48614012632884446 76-77 0.46071176718588414 78-79 0.4466741785883763 80-81 0.4441218897524658 82-83 0.4563161586351494 84-85 0.458537595214553 86-87 0.45976647502443585 88-89 0.46744697383620354 90-91 0.4868727277539669 92-93 0.49145739473699135 94-95 0.4902757795351809 96-97 0.4882670336921032 98-99 0.4850766726472151 100-101 0.46600540328999485 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2115748.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.93852834120466 #Duplication Level Percentage of deduplicated Percentage of total 1 79.71556208652216 50.17180163617634 2 12.075580136571954 15.200384853242438 3 3.7095952906451726 7.004294050040116 4 1.5721740989653483 3.9580129634015386 5 0.8310506495564541 2.6152552430042717 6 0.48435425808958127 1.8290726519972562 7 0.31697739727466445 1.396506363132493 8 0.22542748204196925 1.1350459173907907 9 0.16572837724442968 0.9387630151326355 >10 0.8180157850295723 9.107025289236482 >50 0.054861769907897956 2.3678367717241193 >100 0.029241882220930777 3.229981273506883 >500 8.283497488851819E-4 0.3273137257242569 >1k 6.024361810072988E-4 0.7187062462904602 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4132 0.1952973605552268 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3413 0.16131410735115903 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2211 0.10450204844811387 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 4.726460807241694E-5 0.0 14-15 0.0 0.0 0.0 4.726460807241694E-5 2.363230403620847E-5 16-17 0.0 0.0 0.0 4.726460807241694E-5 4.726460807241694E-5 18-19 0.0 0.0 0.0 4.726460807241694E-5 4.726460807241694E-5 20-21 0.0 0.0 0.0 7.089691210862541E-5 4.726460807241694E-5 22-23 0.0 0.0 0.0 1.654261282534593E-4 4.726460807241694E-5 24-25 0.0 0.0 0.0 2.8358764843450164E-4 4.726460807241694E-5 26-27 0.0 0.0 0.0 5.671752968690033E-4 4.726460807241694E-5 28-29 0.0 0.0 0.0 0.0016306289784983846 7.089691210862541E-5 30-31 0.0 0.0 0.0 0.004891886935495153 9.452921614483389E-5 32-33 0.0 0.0 0.0 0.011532564369669734 9.452921614483389E-5 34-35 0.0 0.0 0.0 0.022143468881927336 9.452921614483389E-5 36-37 0.0 0.0 0.0 0.04003312303733715 9.452921614483389E-5 38-39 0.0 0.0 0.0 0.06761202184759244 9.452921614483389E-5 40-41 0.0 0.0 0.0 0.09828675248659102 9.452921614483389E-5 42-43 0.0 0.0 0.0 0.13191552113011568 9.452921614483389E-5 44-45 0.0 0.0 4.726460807241694E-5 0.17398102231456677 9.452921614483389E-5 46-47 0.0 0.0 4.726460807241694E-5 0.22672832492338407 9.452921614483389E-5 48-49 0.0 0.0 4.726460807241694E-5 0.2843911467717327 9.452921614483389E-5 50-51 0.0 0.0 4.726460807241694E-5 0.3481747353654594 9.452921614483389E-5 52-53 0.0 0.0 4.726460807241694E-5 0.4151959496121466 9.452921614483389E-5 54-55 0.0 0.0 4.726460807241694E-5 0.4917646146894621 9.452921614483389E-5 56-57 0.0 0.0 4.726460807241694E-5 0.5818273253714525 9.452921614483389E-5 58-59 0.0 0.0 4.726460807241694E-5 0.6866365937720371 9.452921614483389E-5 60-61 0.0 0.0 4.726460807241694E-5 0.7998116978014396 9.452921614483389E-5 62-63 0.0 0.0 4.726460807241694E-5 0.9186585547995318 9.452921614483389E-5 64-65 0.0 0.0 4.726460807241694E-5 1.0441224569277627 9.452921614483389E-5 66-67 0.0 0.0 4.726460807241694E-5 1.1959127457523298 9.452921614483389E-5 68-69 0.0 0.0 4.726460807241694E-5 1.3646001319627858 9.452921614483389E-5 70-71 0.0 0.0 4.726460807241694E-5 1.5576524236345728 9.452921614483389E-5 72-73 0.0 0.0 4.726460807241694E-5 1.7607957091298208 9.452921614483389E-5 74-75 0.0 0.0 4.726460807241694E-5 1.9670820910618845 9.452921614483389E-5 76-77 0.0 0.0 4.726460807241694E-5 2.2054611418751193 9.452921614483389E-5 78-79 0.0 0.0 4.726460807241694E-5 2.4708282838977045 9.452921614483389E-5 80-81 0.0 4.726460807241694E-5 4.726460807241694E-5 2.768831637794293 9.452921614483389E-5 82-83 0.0 4.726460807241694E-5 4.726460807241694E-5 3.0816288140175487 9.452921614483389E-5 84-85 0.0 4.726460807241694E-5 4.726460807241694E-5 3.4052732177934235 9.452921614483389E-5 86-87 0.0 4.726460807241694E-5 4.726460807241694E-5 3.750139430593814 9.452921614483389E-5 88-89 0.0 4.726460807241694E-5 4.726460807241694E-5 4.132037463818943 9.452921614483389E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4840 0.0 34.41775 1 GTATCAA 7110 0.0 31.37249 1 ATCAACG 8445 0.0 25.661814 3 TATCAAC 8580 0.0 25.47912 2 TCAACGC 8595 0.0 25.213966 4 CAACGCA 8585 0.0 25.132862 5 AACGCAG 8890 0.0 24.467157 6 GTACATG 6325 0.0 22.960516 1 TACATGG 6455 0.0 21.745344 2 ACGCAGA 10080 0.0 21.390623 7 CGCAGAG 10110 0.0 21.186531 8 ACATGGG 6495 0.0 20.808296 3 CATGGGG 4130 0.0 19.519547 4 GAGTACT 5280 0.0 19.518967 12-13 GCAGAGT 10855 0.0 19.207678 9 GTACTTT 5480 0.0 18.681095 14-15 AGAGTAC 8770 0.0 18.234346 10-11 AGTACTT 5455 0.0 17.850424 12-13 TAACCCG 135 0.009493464 17.563337 5 CAGAGTA 10630 0.0 16.91588 10-11 >>END_MODULE