FastQCFastQC Report
Fri 27 May 2016
SRR939283_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939283_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2115748
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40050.18929475533002985No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36550.17275214250468393No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23230.10979568455222455No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45150.034.1569061
GTATCAA66900.033.1551551
ATCAACG82000.026.5622023
TCAACGC83700.026.1360974
CAACGCA83700.026.0794035
TATCAAC85450.025.8229662
AACGCAG86050.025.3120356
ACGCAGA97750.022.2338147
CGCAGAG99000.021.8572188
GTACATG63250.021.4474471
TACATGG62950.020.9562852
AGAGTAC89050.019.77063610-11
GCAGAGT107800.019.7212649
GAGTACT51650.019.38643312-13
AGTACTT54650.019.36423912-13
ACATGGG65750.018.8369243
GTACTTT56050.017.99157114-15
TAGACCG1851.5336384E-417.9552545
CGCGGAA3100.017.69349782-83
CAGAGTA103400.017.11862610-11
Produced by FastQC (version 0.11.5)