##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939283_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2115748 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.3078797663994 33.0 31.0 34.0 31.0 34.0 2 32.53240130677188 34.0 31.0 34.0 31.0 34.0 3 32.62412678636586 34.0 31.0 34.0 31.0 34.0 4 36.14374916105321 37.0 35.0 37.0 35.0 37.0 5 36.08290354049726 37.0 35.0 37.0 35.0 37.0 6 36.10509710986375 37.0 35.0 37.0 35.0 37.0 7 36.049129669506954 37.0 35.0 37.0 35.0 37.0 8 36.018558448359634 37.0 35.0 37.0 35.0 37.0 9 37.731232169426605 39.0 38.0 39.0 35.0 39.0 10-11 37.6524380502782 39.0 37.0 39.0 35.0 39.0 12-13 37.58223970907689 39.0 37.0 39.0 35.0 39.0 14-15 38.899707337546815 40.0 38.0 41.0 35.0 41.0 16-17 38.89533772453052 40.0 38.0 41.0 35.0 41.0 18-19 38.9798310101203 40.0 38.5 41.0 35.0 41.0 20-21 38.95894147129053 40.0 38.0 41.0 35.0 41.0 22-23 38.88496077982822 40.0 38.0 41.0 35.0 41.0 24-25 38.827933194312365 40.0 38.0 41.0 34.5 41.0 26-27 38.68333728780554 40.0 38.0 41.0 34.0 41.0 28-29 38.52518801861091 40.0 38.0 41.0 34.0 41.0 30-31 38.35518714894212 40.0 38.0 41.0 34.0 41.0 32-33 38.325588633428936 40.0 38.0 41.0 34.0 41.0 34-35 38.24692071078408 40.0 38.0 41.0 34.0 41.0 36-37 38.1080958129229 40.0 38.0 41.0 33.0 41.0 38-39 37.99716837733038 40.0 38.0 41.0 33.0 41.0 40-41 37.83040726022192 40.0 37.0 41.0 33.0 41.0 42-43 37.672086656823026 40.0 37.0 41.0 33.0 41.0 44-45 37.46195860754683 40.0 37.0 41.0 32.0 41.0 46-47 37.2068493034142 40.0 37.0 41.0 31.5 41.0 48-49 36.94241351049369 40.0 36.0 41.0 31.0 41.0 50-51 36.71768282423049 39.5 36.0 41.0 30.5 41.0 52-53 36.47042535311389 39.0 35.5 41.0 30.0 41.0 54-55 36.22898473731276 39.0 35.0 40.0 30.0 41.0 56-57 35.92130182800598 38.5 35.0 40.0 29.0 41.0 58-59 35.60741402095145 38.0 35.0 40.0 28.0 41.0 60-61 35.275337611095466 38.0 34.0 40.0 28.0 41.0 62-63 34.92470582507936 37.5 34.0 40.0 28.0 41.0 64-65 34.62028511902174 37.0 34.0 40.0 27.0 41.0 66-67 34.20357386607479 36.5 33.5 39.0 26.0 41.0 68-69 33.73450406192042 36.0 33.0 39.0 26.0 40.0 70-71 33.25310859327293 35.5 32.5 38.5 25.5 40.0 72-73 32.750920950888286 35.0 32.0 38.0 24.5 40.0 74-75 32.210639688658574 35.0 31.5 37.0 22.5 39.0 76-77 30.25895427999932 33.5 29.5 35.5 20.0 37.5 78-79 31.219738598358596 34.0 31.0 36.0 21.0 38.0 80-81 31.21044873964196 34.0 31.0 36.0 21.0 37.0 82-83 30.844809967916785 34.0 31.0 35.0 20.0 37.0 84-85 30.38729777837436 34.0 30.5 35.0 18.0 36.5 86-87 29.773479639352132 34.0 29.5 35.0 11.5 36.0 88-89 29.18099296324515 34.0 29.0 35.0 7.0 35.5 90-91 28.692957526132602 34.0 29.0 35.0 2.0 35.0 92-93 28.310843257325544 33.0 29.0 35.0 2.0 35.0 94-95 27.775397637147712 33.0 27.0 35.0 2.0 35.0 96-97 27.03213757025884 33.0 26.5 34.5 2.0 35.0 98-99 26.020490389214594 32.5 24.5 34.0 2.0 35.0 100-101 21.70265007931001 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19459103397603172 1101 2 0.2492459266278999 1101 3 0.22138375697179669 1101 4 0.18617851129189944 1101 5 0.1640288346212202 1101 6 0.18102131603637872 1101 7 0.25750718437600284 1101 8 0.23661132767139037 1101 9 0.34898826533668625 1101 10-11 0.3341217870664366 1101 12-13 0.30551613022841906 1101 14-15 0.35267972728374986 1101 16-17 0.2797646752058185 1101 18-19 0.2958048895277159 1101 20-21 0.25056910422720335 1101 22-23 0.29362225661432717 1101 24-25 0.37305427648662004 1101 26-27 0.2586471215812267 1101 28-29 0.43983036428691236 1101 30-31 0.46820322086848165 1101 32-33 0.43716408162871545 1101 34-35 0.5152724436564498 1101 36-37 0.574201543623694 1101 38-39 0.527453805341132 1101 40-41 0.6440882234818233 1101 42-43 0.7085057436357118 1101 44-45 0.7826080952320851 1101 46-47 0.7972177356997818 1101 48-49 0.8129265765003595 1101 50-51 0.7315532434905307 1101 52-53 0.7968355345759193 1101 54-55 0.7609461658394991 1101 56-57 0.6622280025718155 1101 58-59 0.6711531600721798 1101 60-61 0.5768078731944399 1101 62-63 0.6599385324851923 1101 64-65 0.6754793261643783 1101 66-67 0.7461159704306581 1101 68-69 0.8572047065132935 1101 70-71 0.8411865444121247 1101 72-73 0.8314241927292301 1101 74-75 0.7519440304117921 1101 76-77 0.8008717935530392 1101 78-79 1.0884687252216985 1101 80-81 1.024253787209755 1101 82-83 1.1388150971123068 1101 84-85 1.175409372362484 1101 86-87 1.0809973202871745 1101 88-89 1.2822235961261939 1101 90-91 1.222987163838475 1101 92-93 1.2034166719617687 1101 94-95 0.9851467679055936 1101 96-97 0.9078246947859618 1101 98-99 0.7522979115753721 1101 100-101 0.5329263535395228 1102 1 0.19363271205128996 1102 2 0.21745662253231757 1102 3 0.22678448632819936 1102 4 0.09419051908545839 1102 5 0.1530448383989551 1102 6 0.23756528575102465 1102 7 0.16842193539086736 1102 8 0.17477873044040138 1102 9 0.2280680778785964 1102 10-11 0.24554099636156934 1102 12-13 0.298117408784627 1102 14-15 0.2471933838671987 1102 16-17 0.22660360172653782 1102 18-19 0.3206344550487614 1102 20-21 0.2896445319006844 1102 22-23 0.3495189126065128 1102 24-25 0.2983026862277782 1102 26-27 0.2569566655152258 1102 28-29 0.28504946882137716 1102 30-31 0.2272770215772013 1102 32-33 0.34449454991678863 1102 34-35 0.3492406900662175 1102 36-37 0.41783534957045276 1102 38-39 0.5772642242647166 1102 40-41 0.5455075518231567 1102 42-43 0.58831868237057 1102 44-45 0.5536197498552511 1102 46-47 0.38890464022076543 1102 48-49 0.3180326665072215 1102 50-51 0.3479759646486187 1102 52-53 0.2708174911399368 1102 54-55 0.25083666317484443 1102 56-57 0.21311727769948874 1102 58-59 0.33585705248616193 1102 60-61 0.23055405875572887 1102 62-63 0.2591992555718292 1102 64-65 0.3104826792990636 1102 66-67 0.17189016756323383 1102 68-69 0.25542516420625816 1102 70-71 -0.010865316081599019 1102 72-73 0.1295143940363488 1102 74-75 0.1553296051867008 1102 76-77 0.22959734443933755 1102 78-79 0.24601347667306683 1102 80-81 0.4581866173744764 1102 82-83 0.4684545260079922 1102 84-85 0.350424153932547 1102 86-87 0.08927351144363271 1102 88-89 0.2218036804902681 1102 90-91 0.3914653168763351 1102 92-93 0.5054477985753394 1102 94-95 0.4535266086217753 1102 96-97 0.04274669968272704 1102 98-99 -0.1833834096787399 1102 100-101 -0.03804551981119175 1103 1 0.2052996436747705 1103 2 0.23581960158895043 1103 3 0.27656398338128696 1103 4 0.18062090315507362 1103 5 0.20558871819098812 1103 6 0.16510990153420835 1103 7 0.2654711996941259 1103 8 0.16840063118169013 1103 9 0.246796108542938 1103 10-11 0.24245204152786926 1103 12-13 0.34547402298984764 1103 14-15 0.3353297954098977 1103 16-17 0.31514848835445974 1103 18-19 0.3020926301439957 1103 20-21 0.34522511115515186 1103 22-23 0.3812960662638005 1103 24-25 0.4044362892282649 1103 26-27 0.3599578902044911 1103 28-29 0.3437131149803818 1103 30-31 0.3881242062638748 1103 32-33 0.4087480488186941 1103 34-35 0.4495158035560465 1103 36-37 0.37114554334220173 1103 38-39 0.38247827992920236 1103 40-41 0.4271035044606535 1103 42-43 0.3898483663654133 1103 44-45 0.431922142120861 1103 46-47 0.4905994806284397 1103 48-49 0.5481086502329902 1103 50-51 0.5014742777569126 1103 52-53 0.3555918951343102 1103 54-55 0.5089001821545835 1103 56-57 0.6178814955764196 1103 58-59 0.5447725548148341 1103 60-61 0.6446322445669992 1103 62-63 0.5404567994810563 1103 64-65 0.5272475271844144 1103 66-67 0.46818253857342285 1103 68-69 0.36121505934223563 1103 70-71 0.3769696334882866 1103 72-73 0.43053918810603165 1103 74-75 0.2695532228481312 1103 76-77 0.4305023191506798 1103 78-79 0.38002274427355687 1103 80-81 0.45946576998371214 1103 82-83 0.3333736553010276 1103 84-85 0.4077070807043732 1103 86-87 0.5040626647005411 1103 88-89 0.7009670351967827 1103 90-91 0.8672924237245851 1103 92-93 0.6516745191754367 1103 94-95 0.6459794610290537 1103 96-97 0.3727680728655649 1103 98-99 0.2639939594767533 1103 100-101 0.04177639977147152 1104 1 0.06360829911389487 1104 2 0.051360903595124796 1104 3 0.030459163277271273 1104 4 -0.03477245122331851 1104 5 0.08210537295575193 1104 6 0.06272621571768866 1104 7 0.025322600694458686 1104 8 0.095014488831076 1104 9 0.10472902777617321 1104 10-11 0.06845277144753936 1104 12-13 0.07986516509858888 1104 14-15 0.005972464770174213 1104 16-17 0.046443099545314226 1104 18-19 0.06610466902898082 1104 20-21 0.11040267465073583 1104 22-23 0.06723448001042698 1104 24-25 0.09239120184145833 1104 26-27 0.1307790402494433 1104 28-29 0.16994589613236855 1104 30-31 0.0861988515655483 1104 32-33 0.19204105456944376 1104 34-35 0.16132900734300648 1104 36-37 0.2900242401769688 1104 38-39 0.23025925709239203 1104 40-41 0.2650260161123086 1104 42-43 0.22720092762190802 1104 44-45 0.17262261115651256 1104 46-47 0.05745502119287238 1104 48-49 0.056987902649652256 1104 50-51 0.14166914331806169 1104 52-53 0.1850020300018116 1104 54-55 0.22868466906273 1104 56-57 0.042630681481306 1104 58-59 0.03493600126177654 1104 60-61 0.1311550729961226 1104 62-63 0.0845818561767544 1104 64-65 0.053681012296969755 1104 66-67 0.10718445964152323 1104 68-69 0.07966052203419594 1104 70-71 -0.09844514910916047 1104 72-73 -0.13641453719033336 1104 74-75 -0.02200095045797923 1104 76-77 0.08245324977430712 1104 78-79 0.08801165275149714 1104 80-81 0.24672352310514967 1104 82-83 0.347534408133658 1104 84-85 0.15946231239399467 1104 86-87 -0.02047034716161278 1104 88-89 0.05130189302768784 1104 90-91 0.08752448932539991 1104 92-93 0.08681380499406544 1104 94-95 -0.22134746203865419 1104 96-97 -0.037056674572173876 1104 98-99 -0.042738615487287746 1104 100-101 -0.28320656158540913 1105 1 0.030312127047757542 1105 2 -0.00410523242065608 1105 3 0.03090029454107679 1105 4 0.0632743996884031 1105 5 0.07483710024794021 1105 6 0.07715282475552243 1105 7 0.12617144348705267 1105 8 0.04917148121197812 1105 9 0.1141870206473996 1105 10-11 0.12297567181862235 1105 12-13 0.12060756361011471 1105 14-15 0.1513864202694961 1105 16-17 0.1060967491443634 1105 18-19 0.06589322626876282 1105 20-21 0.10860050331489646 1105 22-23 0.1006908219883158 1105 24-25 0.024981263639098472 1105 26-27 0.09942500053793424 1105 28-29 0.11725738584650713 1105 30-31 0.14433302337162246 1105 32-33 0.14874806280566588 1105 34-35 0.3016392357912636 1105 36-37 0.14228545362976064 1105 38-39 0.12186776256508125 1105 40-41 0.27290517478491694 1105 42-43 0.3132308778941777 1105 44-45 0.24275428306479085 1105 46-47 0.2996280014641357 1105 48-49 0.2559942256080845 1105 50-51 0.351178663092405 1105 52-53 0.4167439880749413 1105 54-55 0.48881873601352055 1105 56-57 0.4164204863604013 1105 58-59 0.43638785432487026 1105 60-61 0.4368473753941444 1105 62-63 0.4493474087675722 1105 64-65 0.45955757360717797 1105 66-67 0.4768002620258045 1105 68-69 0.644528181500263 1105 70-71 0.4021252035902947 1105 72-73 0.3349413779064463 1105 74-75 0.4280455530917422 1105 76-77 0.6386739281140912 1105 78-79 0.5574226639883371 1105 80-81 0.5735760376803789 1105 82-83 0.6949169638634345 1105 84-85 0.8321914159944832 1105 86-87 0.7735475664630371 1105 88-89 0.8137495895256848 1105 90-91 0.8747100649335628 1105 92-93 0.8545412191311001 1105 94-95 0.747894487373518 1105 96-97 0.5657064700424357 1105 98-99 0.3277021716479389 1105 100-101 0.17615658806409584 1106 1 0.023307322435968558 1106 2 0.06979398632047662 1106 3 0.061784284758580554 1106 4 0.1068713575025626 1106 5 0.029188277214132086 1106 6 0.05521392459358765 1106 7 0.008953882300247074 1106 8 0.0033894751323231276 1106 9 0.03762814984364127 1106 10-11 0.08340906014007032 1106 12-13 0.07070890967199972 1106 14-15 0.07072058967165162 1106 16-17 0.04846891290783617 1106 18-19 -0.024782156537838773 1106 20-21 0.10640298305848717 1106 22-23 0.09367644066922765 1106 24-25 0.16247160063006305 1106 26-27 0.10889124164245345 1106 28-29 0.05990827633551277 1106 30-31 0.09279129598751723 1106 32-33 -0.01539347112895939 1106 34-35 0.19341553905307052 1106 36-37 0.144884304736685 1106 38-39 0.2077350815168657 1106 40-41 0.13296286665146084 1106 42-43 0.17962010957722185 1106 44-45 0.26226047685592135 1106 46-47 0.20420780561379814 1106 48-49 0.30252176721609203 1106 50-51 0.4054669010473049 1106 52-53 0.34849703107369123 1106 54-55 0.17850457769807804 1106 56-57 0.25646787101731405 1106 58-59 0.18734079238072354 1106 60-61 0.3760351726200639 1106 62-63 0.2536760136573477 1106 64-65 0.11265838894131264 1106 66-67 0.20202693489283519 1106 68-69 0.23032857485794977 1106 70-71 -0.01952296555484878 1106 72-73 0.14529729512004508 1106 74-75 0.08552147602463833 1106 76-77 0.2157424801894443 1106 78-79 0.279039252270767 1106 80-81 0.3202928417505255 1106 82-83 0.28046624704689194 1106 84-85 0.16264717149535457 1106 86-87 0.13565901661928592 1106 88-89 0.3654514483645741 1106 90-91 0.3467110021145956 1106 92-93 0.4965334994141486 1106 94-95 0.6050631316302457 1106 96-97 0.5480376677700391 1106 98-99 0.5737121924472106 1106 100-101 0.46641233291923356 1107 1 0.06619912304016395 1107 2 0.07210962528897369 1107 3 0.07040184132613092 1107 4 0.021069029102974923 1107 5 0.038839313426642263 1107 6 0.08647178429774272 1107 7 0.06611819794226648 1107 8 0.051708459299788956 1107 9 -0.005323969736878098 1107 10-11 0.031056543847952867 1107 12-13 0.03569232848625603 1107 14-15 0.004900500926545703 1107 16-17 0.10403129154855861 1107 18-19 0.11688305659392739 1107 20-21 0.1744194882466843 1107 22-23 0.1424893000652503 1107 24-25 0.09684988945691941 1107 26-27 0.10242393681408402 1107 28-29 0.14835102981621873 1107 30-31 0.10026366303697642 1107 32-33 0.05468335172901817 1107 34-35 0.17513028778315487 1107 36-37 0.1920671363446118 1107 38-39 0.07413834806163067 1107 40-41 0.19647994639423416 1107 42-43 0.23423338981243091 1107 44-45 0.18330544105976543 1107 46-47 0.30632293298155844 1107 48-49 0.17386030191790525 1107 50-51 0.2020786077629424 1107 52-53 0.3131674227093413 1107 54-55 0.2294857627503859 1107 56-57 0.0522137717766995 1107 58-59 0.1729984858919451 1107 60-61 0.13391458388072408 1107 62-63 0.15198878083285194 1107 64-65 0.1913999129819075 1107 66-67 0.21436085900372603 1107 68-69 0.3448089834939694 1107 70-71 0.2362218725453218 1107 72-73 0.1734182414180907 1107 74-75 0.12681188402262933 1107 76-77 0.058835946511965886 1107 78-79 0.1430379256888017 1107 80-81 0.23979030846239624 1107 82-83 0.3860261976854993 1107 84-85 0.3169372334758229 1107 86-87 0.34389743466811495 1107 88-89 0.6111421801252135 1107 90-91 0.6310549831626773 1107 92-93 0.45798292304128907 1107 94-95 0.7400543875562597 1107 96-97 0.8209774426474254 1107 98-99 0.6311387976451392 1107 100-101 0.6035978277079579 1108 1 -0.07865207617042813 1108 2 0.03127394202177669 1108 3 -0.035627387172510794 1108 4 0.05829899937258887 1108 5 -0.012920478673322577 1108 6 -0.009160528675167257 1108 7 0.008912513866945915 1108 8 -0.046341414590635566 1108 9 0.03100898505507388 1108 10-11 -0.02971582943637685 1108 12-13 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82-83 -0.16660731077971747 2316 84-85 -0.20492026431497834 2316 86-87 -0.4758170652283944 2316 88-89 -0.83073415980936 2316 90-91 -0.8658807817008132 2316 92-93 -0.45743985281052346 2316 94-95 -0.32536659344197005 2316 96-97 -0.3295938745230593 2316 98-99 -0.23638449153479613 2316 100-101 0.5670437250927414 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 13.0 5 33.0 6 74.0 7 187.0 8 423.0 9 912.0 10 1542.0 11 2347.0 12 3508.0 13 4716.0 14 6126.0 15 8174.0 16 10302.0 17 13846.0 18 14125.0 19 10714.0 20 10642.0 21 11498.0 22 12452.0 23 13980.0 24 16205.0 25 19066.0 26 23262.0 27 28282.0 28 34543.0 29 41768.0 30 50938.0 31 62723.0 32 77934.0 33 99313.0 34 134959.0 35 193094.0 36 303782.0 37 491592.0 38 386902.0 39 25764.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.645768842410654 19.82265687589774 15.658625152289437 22.872949129402173 2 15.5546407228082 20.5581666625704 39.75170956087398 24.13548305374742 3 19.01871111304371 24.049555996271767 30.14739940673464 26.784333483949887 4 13.34431132630162 17.49111898014319 37.12795663755797 32.036613055997215 5 14.165912008424444 37.019106245167194 34.35829786912241 14.456683877285952 6 31.83191003843558 36.516919784397764 17.509977558764085 14.14119261840257 7 28.612197671934464 32.29689925265202 20.73498356136931 18.355919514044206 8 25.949404182350637 35.82316986711083 19.686087379026233 18.5413385715123 9 25.635519920141718 17.35494964428656 20.402618837404077 36.60691159816765 10-11 24.88561964846475 26.843107024087935 27.467118012164022 20.804155315283296 12-13 25.546402501621174 23.841260868496626 27.995276375069245 22.617060254812955 14-15 22.45734843394883 25.611547277415553 25.987920421398016 25.9431838672376 16-17 22.399786739820453 28.76379757203853 26.55089235347402 22.285523334666998 18-19 22.250562626477226 27.517025314705705 28.601299824908132 21.63111223390893 20-21 23.02897675644187 26.685651288728923 28.51992026458734 21.765451690241868 22-23 22.659078491389334 26.51029328634601 28.423281033469017 22.40734718879564 24-25 22.67318697689895 26.709135492506668 28.427487583587457 22.19018994700692 26-27 22.476918306828892 26.91868494611357 28.386157884736633 22.218238862320902 28-29 22.288453067189476 27.031645545688804 28.541277127521802 22.138624259599915 30-31 22.566014478094747 26.675459459255073 28.4116539398832 22.346872122766985 32-33 22.422897244851466 26.826895263519095 28.31854266198054 22.431664829648902 34-35 22.84396050872805 26.868594342886638 28.094283332104432 22.193161816280885 36-37 22.85755409217857 26.646423188740105 28.242960495202613 22.253062223878718 38-39 22.504071365825958 26.91338832404834 28.29322152743986 22.28931878268584 40-41 22.702489110451957 26.738930426712415 28.271112465025944 22.28746799780968 42-43 22.647292227836747 26.729191588959527 28.075101749125487 22.54841443407824 44-45 22.704763723348893 26.921817122217078 27.924147771986735 22.449271382447293 46-47 22.746051859795827 27.040996945281158 27.641991489514556 22.570959705408455 48-49 22.84373856044187 26.98560007033002 27.669072681206735 22.501588688021375 50-51 22.693674004346455 27.080968131335236 27.566730599986954 22.65862726433135 52-53 22.881380165345895 27.12075855968567 27.318182967571964 22.67967830739647 54-55 22.659740195902348 26.968098280135443 27.51575329387054 22.856408230091674 56-57 22.68648300420328 27.248409413492936 27.52226301194529 22.542844570358493 58-59 22.840221723568703 26.995210799275416 27.504593638740886 22.65997383841499 60-61 22.850505499283933 27.014812854192172 27.7106955991549 22.42398604736899 62-63 22.614423786128647 27.066665941933255 27.63850423531842 22.680406036619676 64-65 22.5756251989546 26.852744409597275 27.594421165295557 22.977209226152567 66-67 22.579389593319018 27.104573961697508 27.407990690714396 22.90804575426908 68-69 22.491409503192784 26.964116065056174 27.584569718912327 22.959904712838718 70-71 22.60928522678504 26.943284360897422 27.447834052070476 22.99959636024706 72-73 22.64102341110567 26.877302968028328 27.48382605111762 22.99784756974838 74-75 22.54354015695631 27.132815439267816 27.19910405208938 23.124540351686495 76-77 22.529215341131355 26.97544894870738 27.36668490317787 23.12865080698339 78-79 22.614981447420256 27.02345534796633 27.322522791633297 23.039040412980118 80-81 22.537049597186805 27.22870667306315 27.225492675156975 23.008751054593063 82-83 22.63442999827957 27.11509121124066 27.22701380315614 23.02346498732363 84-85 22.554576442941222 27.004870145215783 27.194353958978102 23.246199452864897 86-87 22.49076515652273 26.906551001839162 27.37606478088332 23.22661906075479 88-89 22.465144844629968 26.85836636407824 27.27065268625414 23.40583610503765 90-91 22.39733729247956 27.012523209230842 27.33408140026755 23.256058098022045 92-93 22.48415217691332 26.768358046421408 27.399435093404318 23.348054683260955 94-95 22.32969143773266 26.821837950455347 27.278106844482426 23.570363767329567 96-97 22.307713394978986 26.76129198751458 27.340330701009762 23.59066391649667 98-99 22.200485738721536 26.801604067406 27.120616765760925 23.877293428111543 100-101 22.38902228062049 26.29195931447153 27.688232013385388 23.630786391522587 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 133.0 1 96.0 2 89.5 3 125.0 4 104.0 5 100.5 6 192.0 7 466.0 8 762.5 9 959.5 10 957.0 11 880.0 12 973.5 13 1184.0 14 1393.0 15 1509.0 16 1673.0 17 1825.0 18 1893.0 19 2099.0 20 2388.0 21 2742.0 22 3324.5 23 4068.0 24 5083.0 25 6723.0 26 8928.5 27 11694.5 28 15024.0 29 18636.5 30 22531.0 31 26908.5 32 31099.5 33 34725.5 34 38693.0 35 42744.5 36 47574.0 37 52682.0 38 56605.0 39 59520.5 40 62367.5 41 67198.0 42 72549.0 43 76684.0 44 79817.5 45 83604.5 46 85694.5 47 87060.5 48 91539.0 49 92159.5 50 88167.0 51 84448.0 52 80435.0 53 77240.5 54 73821.0 55 67179.5 56 59043.5 57 51582.5 58 44747.5 59 37762.5 60 31163.5 61 25492.0 62 20651.5 63 16264.0 64 12549.5 65 9609.5 66 7140.5 67 5588.0 68 4179.5 69 2801.5 70 1985.0 71 1518.5 72 1266.0 73 1008.0 74 783.5 75 643.5 76 431.5 77 225.5 78 119.0 79 77.0 80 42.5 81 23.5 82 17.5 83 9.5 84 6.0 85 3.5 86 1.0 87 0.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29672720947863357 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.001347041330063883 16-17 0.0010634536816293812 18-19 7.089691210862541E-5 20-21 2.363230403620847E-5 22-23 0.0 24-25 0.0 26-27 9.452921614483389E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0018905843228966778 36-37 0.00801135106827467 38-39 0.0027177149641639743 40-41 0.0034266840852502285 42-43 2.3632304036208472E-4 44-45 0.0013706736341000912 46-47 2.3632304036208472E-4 48-49 4.0174916861554407E-4 50-51 9.452921614483389E-5 52-53 7.089691210862541E-5 54-55 0.0 56-57 8.980275533759219E-4 58-59 0.002386862707657056 60-61 8.507629453035049E-4 62-63 0.0012997767219914658 64-65 7.089691210862541E-5 66-67 5.199106887965863E-4 68-69 0.0017960551067518439 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.2605461519991984 78-79 2.1269073632587622E-4 80-81 1.4179382421725082E-4 82-83 0.0 84-85 0.0 86-87 0.1079996294454727 88-89 0.37762058619457517 90-91 0.2772305586487616 92-93 0.0 94-95 0.0 96-97 0.0 98-99 7.089691210862541E-5 100-101 3.7811686457933554E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2115748.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.381598338264695 #Duplication Level Percentage of deduplicated Percentage of total 1 77.74518994214912 46.943788318189455 2 13.070255249735075 15.784058053361976 3 4.113052198464392 7.450579973759805 4 1.8041510924601771 4.357501064258875 5 0.9195737617047797 2.776266676083257 6 0.5492587262443608 1.9899071875124332 7 0.36083685751224337 1.5251534337162151 8 0.246056974698917 1.1885850731678853 9 0.17826668393971976 0.9687624576068288 >10 0.917261103235717 9.84582939038781 >50 0.06115750340375939 2.5464331347464992 >100 0.0334493029559775 3.5472910031453737 >500 9.414337868017246E-4 0.38839535357702204 >1k 5.491697089676294E-4 0.6874488804865733 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4005 0.18929475533002985 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3655 0.17275214250468393 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2323 0.10979568455222455 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.363230403620847E-5 0.0 12-13 0.0 0.0 0.0 4.726460807241694E-5 0.0 14-15 0.0 0.0 0.0 7.089691210862541E-5 0.0 16-17 0.0 0.0 0.0 1.4179382421725082E-4 0.0 18-19 0.0 0.0 0.0 1.8905843228966777E-4 0.0 20-21 0.0 0.0 0.0 2.1269073632587625E-4 0.0 22-23 0.0 0.0 0.0 3.072199524707101E-4 0.0 24-25 0.0 0.0 0.0 4.726460807241694E-4 0.0 26-27 0.0 0.0 0.0 7.562337291586711E-4 0.0 28-29 0.0 0.0 0.0 0.0017960551067518439 0.0 30-31 0.0 0.0 0.0 0.005199106887965864 0.0 32-33 0.0 0.0 0.0 0.012028842754430112 0.0 34-35 0.0 0.0 0.0 0.023136025651448094 0.0 36-37 0.0 0.0 0.0 0.04130926745529241 0.0 38-39 0.0 0.0 0.0 0.06940807695434428 0.0 40-41 0.0 0.0 0.0 0.10039002754581358 0.0 42-43 0.0 0.0 0.0 0.13437328074988136 0.0 44-45 0.0 0.0 0.0 0.17700595723120144 0.0 46-47 0.0 0.0 0.0 0.23029680283285153 0.0 48-49 0.0 0.0 0.0 0.28807778620138125 0.0 50-51 0.0 0.0 0.0 0.35249944700408553 0.0 52-53 0.0 0.0 0.0 0.4203714241960763 0.0 54-55 0.0 0.0 0.0 0.49760179378640557 0.0 56-57 0.0 0.0 0.0 0.5886097966298445 0.0 58-59 0.0 0.0 0.0 0.6946006802322394 0.0 60-61 0.0 0.0 0.0 0.8095009424562849 0.0 62-63 0.0 0.0 0.0 0.9291512977916085 0.0 64-65 0.0 0.0 0.0 1.0557495505135772 0.0 66-67 0.0 0.0 0.0 1.2085560284117012 0.0 68-69 2.363230403620847E-5 0.0 0.0 1.37778695761499 0.0 70-71 4.726460807241694E-5 0.0 0.0 1.5713827922796098 0.0 72-73 4.726460807241694E-5 0.0 0.0 1.776014912929139 0.0 74-75 4.726460807241694E-5 0.0 0.0 1.9838846592316286 0.0 76-77 4.726460807241694E-5 0.0 0.0 2.2226181946054067 0.0 78-79 4.726460807241694E-5 0.0 0.0 2.4907266838961917 0.0 80-81 4.726460807241694E-5 0.0 0.0 2.7901007114268808 0.0 82-83 4.726460807241694E-5 0.0 0.0 3.104481252020562 0.0 84-85 4.726460807241694E-5 0.0 0.0 3.4301107693354784 0.0 86-87 4.726460807241694E-5 0.0 0.0 3.7774111094515983 0.0 88-89 4.726460807241694E-5 0.0 0.0 4.160987036263298 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4515 0.0 34.156906 1 GTATCAA 6690 0.0 33.155155 1 ATCAACG 8200 0.0 26.562202 3 TCAACGC 8370 0.0 26.136097 4 CAACGCA 8370 0.0 26.079403 5 TATCAAC 8545 0.0 25.822966 2 AACGCAG 8605 0.0 25.312035 6 ACGCAGA 9775 0.0 22.233814 7 CGCAGAG 9900 0.0 21.857218 8 GTACATG 6325 0.0 21.447447 1 TACATGG 6295 0.0 20.956285 2 AGAGTAC 8905 0.0 19.770636 10-11 GCAGAGT 10780 0.0 19.721264 9 GAGTACT 5165 0.0 19.386433 12-13 AGTACTT 5465 0.0 19.364239 12-13 ACATGGG 6575 0.0 18.836924 3 GTACTTT 5605 0.0 17.991571 14-15 TAGACCG 185 1.5336384E-4 17.955254 5 CGCGGAA 310 0.0 17.693497 82-83 CAGAGTA 10340 0.0 17.118626 10-11 >>END_MODULE