FastQCFastQC Report
Fri 27 May 2016
SRR939282_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939282_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2154597
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43180.20040870752163864No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35150.16313955695659096No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21850.10141107594598897No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46700.034.2857361
GTATCAA71450.033.2482071
ATCAACG85700.027.5607683
TCAACGC86600.027.274344
CAACGCA87450.026.9555
TATCAAC88700.026.6820832
AACGCAG89600.026.2375856
ACGCAGA100700.023.2042587
GTACATG66750.022.8483181
TACATGG65500.022.8093052
CGCAGAG102500.022.7042858
ACATGGG67600.021.7499263
GAGTACT51300.020.7424112-13
GTACCGT1856.6808498E-620.4959686
GCAGAGT108750.020.4837139
CATGGGG43850.020.1180634
GTGTAGC12100.020.0255871
AGAGTAC88300.019.75296410-11
GTACTTT55800.018.98491514-15
AGTACTT55600.018.75461612-13
Produced by FastQC (version 0.11.5)