##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939282_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2154597 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.46048889885208 33.0 31.0 34.0 30.0 34.0 2 31.564729274198378 33.0 31.0 34.0 28.0 34.0 3 31.61052670174515 33.0 31.0 34.0 28.0 34.0 4 35.1054614853729 37.0 35.0 37.0 32.0 37.0 5 35.142616925578196 37.0 35.0 37.0 32.0 37.0 6 35.21752281285085 37.0 35.0 37.0 32.0 37.0 7 35.11913643247438 37.0 35.0 37.0 32.0 37.0 8 35.09162409490035 37.0 35.0 37.0 32.0 37.0 9 36.65970109491473 39.0 37.0 39.0 32.0 39.0 10-11 36.55075543129411 39.0 37.0 39.0 32.0 39.0 12-13 36.42653127243749 39.0 37.0 39.0 32.0 39.0 14-15 37.594546683208044 40.0 37.5 41.0 32.0 41.0 16-17 37.50871207005301 40.0 37.0 41.0 32.0 41.0 18-19 37.42095505563221 40.0 37.0 41.0 32.0 41.0 20-21 37.299837742278484 40.0 37.0 41.0 31.0 41.0 22-23 37.21085706514954 40.0 37.0 41.0 31.0 41.0 24-25 37.2348569129169 40.0 37.0 41.0 31.0 41.0 26-27 37.14947621295305 40.0 37.0 41.0 31.0 41.0 28-29 36.9965937945704 40.0 37.0 41.0 30.0 41.0 30-31 36.82218855776742 40.0 36.5 41.0 30.0 41.0 32-33 36.626698867584054 40.0 36.0 41.0 30.0 41.0 34-35 36.31171467332406 39.0 35.5 41.0 29.5 41.0 36-37 36.055442386673704 39.0 35.0 41.0 28.5 41.0 38-39 35.73153819484572 39.0 35.0 40.5 26.5 41.0 40-41 35.60474696660211 39.0 35.0 40.0 27.0 41.0 42-43 35.451069968072915 39.0 35.0 40.0 26.0 41.0 44-45 35.43256302686767 39.0 35.0 41.0 26.0 41.0 46-47 35.274908254304634 39.0 35.0 41.0 24.5 41.0 48-49 35.1018816975982 39.0 35.0 41.0 24.0 41.0 50-51 33.994152038641104 38.0 33.5 40.0 23.0 40.5 52-53 34.12373984554884 38.0 33.5 39.5 23.0 40.5 54-55 34.61846414898007 38.5 34.0 40.0 23.0 41.0 56-57 34.51413489390359 38.5 34.0 40.0 23.0 41.0 58-59 34.307328702304886 38.0 34.0 40.0 22.5 41.0 60-61 34.01268427459985 38.0 34.0 40.0 22.0 41.0 62-63 33.64370181523505 37.5 33.0 40.0 20.5 41.0 64-65 33.26126045845233 37.0 33.0 40.0 19.0 41.0 66-67 32.892842837894975 36.5 32.5 39.5 18.0 41.0 68-69 32.46509231192655 36.0 32.0 39.0 14.5 41.0 70-71 32.048742989988384 35.5 31.5 39.0 12.0 40.0 72-73 31.591537767851715 35.0 31.0 38.0 8.0 40.0 74-75 31.118001417434442 35.0 31.0 37.0 7.0 39.0 76-77 30.646659909022432 35.0 30.5 37.0 4.0 39.0 78-79 30.1977334972619 34.5 30.0 36.0 2.0 39.0 80-81 29.71425909346388 34.0 29.5 36.0 2.0 37.5 82-83 29.250643391780457 34.0 29.0 35.0 2.0 37.0 84-85 28.807193642244933 34.0 29.0 35.0 2.0 36.5 86-87 28.443511245954582 34.0 29.0 35.0 2.0 36.0 88-89 28.00210132103591 34.0 27.5 35.0 2.0 36.0 90-91 27.57999291746902 34.0 26.5 35.0 2.0 35.0 92-93 27.138826193483048 33.5 26.0 35.0 2.0 35.0 94-95 26.61849547734449 33.0 25.0 35.0 2.0 35.0 96-97 26.00425392776468 33.0 24.0 35.0 2.0 35.0 98-99 25.205096127025147 32.5 19.0 35.0 2.0 35.0 100-101 22.71702852087885 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5203507355480284 1101 2 0.3183182811596019 1101 3 0.5579424277625691 1101 4 0.6394941564045737 1101 5 0.638964026365521 1101 6 0.5933460551768164 1101 7 0.6001888180953614 1101 8 0.5558088992276637 1101 9 0.6868552069422904 1101 10-11 0.7570723258221861 1101 12-13 0.7066257897693262 1101 14-15 0.8109843335891611 1101 16-17 0.917460317940801 1101 18-19 0.9564253616144143 1101 20-21 0.839715077135061 1101 22-23 0.8811521756339644 1101 24-25 0.9654490819357733 1101 26-27 0.9449987621495666 1101 28-29 0.8223274374948133 1101 30-31 0.9195392029192462 1101 32-33 0.9161296122685911 1101 34-35 1.1125004090418642 1101 36-37 0.9994867631654358 1101 38-39 0.9282120254070918 1101 40-41 0.9952070927157024 1101 42-43 0.7869139580170241 1101 44-45 0.9086675672634783 1101 46-47 0.9669932559942325 1101 48-49 1.0453960868183358 1101 50-51 1.0775015498477032 1101 52-53 1.1414793834720385 1101 54-55 1.0608522607711208 1101 56-57 1.1952813864363279 1101 58-59 1.1384071196573302 1101 60-61 1.1674661094114853 1101 62-63 1.2619520712356476 1101 64-65 1.2296668707953913 1101 66-67 1.2901356525817178 1101 68-69 1.1164260337738696 1101 70-71 1.0066209508157016 1101 72-73 1.039011745832564 1101 74-75 0.9941957859410557 1101 76-77 0.9797322042127377 1101 78-79 1.0894949704698789 1101 80-81 0.8440638460893339 1101 82-83 0.7388426266277293 1101 84-85 0.7346976784197885 1101 86-87 1.001441301200284 1101 88-89 0.7145222423571553 1101 90-91 0.838212738346499 1101 92-93 0.8455312370638985 1101 94-95 0.9669183228355074 1101 96-97 0.787395877940579 1101 98-99 0.6302582263934049 1101 100-101 0.5460796387347813 1102 1 0.44107311198813903 1102 2 0.5076523447173713 1102 3 0.6119064286233282 1102 4 0.5226622357666955 1102 5 0.5092415539398303 1102 6 0.5074758262404444 1102 7 0.4995897101149325 1102 8 0.6431110127534723 1102 9 0.7173333802769264 1102 10-11 0.5751818120934971 1102 12-13 0.6054567524663597 1102 14-15 0.7588286196108669 1102 16-17 0.8334427781923637 1102 18-19 0.77437985870624 1102 20-21 0.7371859649011085 1102 22-23 0.7002661593441815 1102 24-25 0.8401606165536535 1102 26-27 0.9163398865116861 1102 28-29 0.9230000306923785 1102 30-31 0.9134449565723841 1102 32-33 0.9216244873329558 1102 34-35 0.82974569001032 1102 36-37 1.0016788726722936 1102 38-39 1.241116747634166 1102 40-41 1.2328292020478173 1102 42-43 1.0366071512513102 1102 44-45 1.0422311383194867 1102 46-47 0.9732216667196312 1102 48-49 0.96446767889352 1102 50-51 0.8832892168260997 1102 52-53 0.9116142731281229 1102 54-55 0.8303849067583613 1102 56-57 0.7987954878186372 1102 58-59 0.7943280294569774 1102 60-61 0.7305492187984228 1102 62-63 0.6685043674571958 1102 64-65 0.8521459029735396 1102 66-67 0.8925419702997246 1102 68-69 0.8182300838864833 1102 70-71 0.7739395853502558 1102 72-73 0.8867151891774903 1102 74-75 0.7024800593146807 1102 76-77 0.6596069011460592 1102 78-79 0.5611019102510184 1102 80-81 0.716582912152063 1102 82-83 0.58129462965044 1102 84-85 0.7062561466814756 1102 86-87 0.5724764924808667 1102 88-89 0.4918987770193226 1102 90-91 0.40053681964364785 1102 92-93 0.618533965112821 1102 94-95 0.7871423641825466 1102 96-97 0.6869528362845152 1102 98-99 0.5469325727041046 1102 100-101 0.7183099581608943 1103 1 0.43819697446544836 1103 2 0.5194901759889206 1103 3 0.5104782552701508 1103 4 0.4423769317416628 1103 5 0.43604045169198713 1103 6 0.4531668072587536 1103 7 0.4540823332438819 1103 8 0.40527044479058105 1103 9 0.5365016244984702 1103 10-11 0.609363498975469 1103 12-13 0.45495598012136185 1103 14-15 0.6898589424479553 1103 16-17 0.7003489392939244 1103 18-19 0.6989186260642342 1103 20-21 0.7997691504222857 1103 22-23 0.780146901705848 1103 24-25 0.7281873643450965 1103 26-27 0.688422879515656 1103 28-29 0.6967485910063331 1103 30-31 0.6947015679868898 1103 32-33 0.7596606153963137 1103 34-35 0.7753197470773543 1103 36-37 0.7399647340028821 1103 38-39 0.8627569510722566 1103 40-41 0.8353832476285135 1103 42-43 0.7725768161947002 1103 44-45 0.6151495146195813 1103 46-47 0.6648748779473053 1103 48-49 0.7025925301928453 1103 50-51 0.5632556634856627 1103 52-53 0.717110681190789 1103 54-55 0.7742336167493491 1103 56-57 0.7956550734142738 1103 58-59 0.7100766554208562 1103 60-61 0.8008641203966178 1103 62-63 0.7266383330743622 1103 64-65 0.6429065388002329 1103 66-67 0.5788824494914522 1103 68-69 0.6787647282376028 1103 70-71 0.5519098670195817 1103 72-73 0.46066108261903693 1103 74-75 0.46516025817165385 1103 76-77 0.4592157809451223 1103 78-79 0.35766513675786626 1103 80-81 0.4443017713864066 1103 82-83 0.4206716693803827 1103 84-85 0.4197338842892755 1103 86-87 0.3995495625228678 1103 88-89 0.4971927005917891 1103 90-91 0.4767139718889375 1103 92-93 0.4449615574402124 1103 94-95 0.4366391000156433 1103 96-97 0.03028037213860202 1103 98-99 0.06326526734833848 1103 100-101 0.027377817899115087 1104 1 0.37517685376789345 1104 2 0.4370513593416945 1104 3 0.4358721879672345 1104 4 0.46802440265531686 1104 5 0.44557225516798127 1104 6 0.4567943217035122 1104 7 0.4435100255602009 1104 8 0.5338163997311938 1104 9 0.4711178258544919 1104 10-11 0.5366050625164291 1104 12-13 0.6280735470515495 1104 14-15 0.6599532068962901 1104 16-17 0.7351341116183789 1104 18-19 0.6823287279257144 1104 20-21 0.6137931567746406 1104 22-23 0.7523421483256669 1104 24-25 0.8527729089056137 1104 26-27 0.6864896301733268 1104 28-29 0.7603248231242716 1104 30-31 0.8524566299323979 1104 32-33 0.8389667060837027 1104 34-35 0.824123108835721 1104 36-37 0.9630643479136793 1104 38-39 1.0423648206854423 1104 40-41 0.891331140186999 1104 42-43 1.0415542790230745 1104 44-45 1.004502731690856 1104 46-47 1.1013193204190301 1104 48-49 1.1252212073204646 1104 50-51 1.1060174896429658 1104 52-53 1.1857805014526832 1104 54-55 1.2006024089117844 1104 56-57 1.2300457231734114 1104 58-59 1.214492373996002 1104 60-61 1.372945785259411 1104 62-63 1.3201183150489229 1104 64-65 1.2830671745216762 1104 66-67 1.2783750693637757 1104 68-69 1.361482429914723 1104 70-71 1.2602238787613445 1104 72-73 1.1597904371022842 1104 74-75 1.1623778555413757 1104 76-77 1.2383699309192941 1104 78-79 1.310597155670095 1104 80-81 1.4117795905886403 1104 82-83 1.2567447167938752 1104 84-85 1.0646764241147224 1104 86-87 0.9190310998806801 1104 88-89 0.9096282209852262 1104 90-91 0.9870030260136105 1104 92-93 1.0909373595266878 1104 94-95 0.9725060089215312 1104 96-97 0.9751674059470439 1104 98-99 0.881747743122272 1104 100-101 0.6117529208340429 1105 1 0.2925275018046989 1105 2 0.3584789946568847 1105 3 0.3872840276008347 1105 4 0.29130613369647307 1105 5 0.3850950278504186 1105 6 0.3844489503900377 1105 7 0.39893815290972157 1105 8 0.3463317758040674 1105 9 0.5551798221803281 1105 10-11 0.5401940391080089 1105 12-13 0.4396948691523974 1105 14-15 0.4382098878717997 1105 16-17 0.5409151701359747 1105 18-19 0.6018636405206479 1105 20-21 0.6437179261048058 1105 22-23 0.7007223437138848 1105 24-25 0.598934236386647 1105 26-27 0.544059601364026 1105 28-29 0.5356734432438728 1105 30-31 0.6370563002234135 1105 32-33 0.548559881061685 1105 34-35 0.698983488345938 1105 36-37 0.7178937568516872 1105 38-39 0.9695693759116963 1105 40-41 0.9010652577096252 1105 42-43 0.6848174323193632 1105 44-45 0.7448555249053825 1105 46-47 0.8244532112379943 1105 48-49 0.8083277603440635 1105 50-51 0.6583430416296068 1105 52-53 0.7601920720768618 1105 54-55 0.7354482113301088 1105 56-57 0.6468294970767374 1105 58-59 0.6877728404629408 1105 60-61 0.7197649212563206 1105 62-63 0.8194523442812809 1105 64-65 0.6729554091530758 1105 66-67 0.7693181888154541 1105 68-69 0.7711744716042901 1105 70-71 0.6817553241236922 1105 72-73 0.7092717317503734 1105 74-75 0.8101069101686811 1105 76-77 0.6870936306495956 1105 78-79 0.626718177721969 1105 80-81 0.6117978694341559 1105 82-83 0.7173850741855432 1105 84-85 0.8249599416543667 1105 86-87 0.7348935739856479 1105 88-89 0.7169033164605807 1105 90-91 0.7684021739205136 1105 92-93 0.8312074955718813 1105 94-95 0.7832616724896084 1105 96-97 0.7620333081977542 1105 98-99 0.5992630157299885 1105 100-101 0.3041081098419056 1106 1 0.2760573892634248 1106 2 0.3111465149300372 1106 3 0.22413961086116174 1106 4 0.28254094287049725 1106 5 0.3914004841929426 1106 6 0.292115684446685 1106 7 0.23502773666424304 1106 8 0.246111635183631 1106 9 0.4142471447761906 1106 10-11 0.29602327288387187 1106 12-13 0.4417480173879085 1106 14-15 0.4915007655140471 1106 16-17 0.5371705332556829 1106 18-19 0.562900997036138 1106 20-21 0.5820819218817022 1106 22-23 0.6582688989624401 1106 24-25 0.6290317272328423 1106 26-27 0.6426837224862965 1106 28-29 0.5799363091153396 1106 30-31 0.6162204397656126 1106 32-33 0.4698450080918093 1106 34-35 0.6070365916547829 1106 36-37 0.5221191089643682 1106 38-39 0.7771597346510575 1106 40-41 0.7303697930556794 1106 42-43 0.5598943692198759 1106 44-45 0.5523133500059103 1106 46-47 0.44856197867515135 1106 48-49 0.5338775503119422 1106 50-51 0.6791609314245193 1106 52-53 0.4349713698732032 1106 54-55 0.6563435996087463 1106 56-57 0.7297236723356306 1106 58-59 0.6394351267805263 1106 60-61 0.7230108722413391 1106 62-63 0.6891740236397297 1106 64-65 0.7215885156067898 1106 66-67 0.5403461107329264 1106 68-69 0.5283088247727434 1106 70-71 0.618285189119085 1106 72-73 0.47712895483369167 1106 74-75 0.4408041718889244 1106 76-77 0.6606857415285852 1106 78-79 0.6222535911595344 1106 80-81 0.6200149550418992 1106 82-83 0.6715688691402804 1106 84-85 0.7625186271003805 1106 86-87 0.9992072069797864 1106 88-89 1.0349043048470392 1106 90-91 1.1028395798553206 1106 92-93 1.0546861666413605 1106 94-95 1.0149675376647096 1106 96-97 0.9114447689880159 1106 98-99 0.9226574493171533 1106 100-101 0.8168178611728578 1107 1 0.11005120455368811 1107 2 0.10075507650861226 1107 3 0.12576634543836462 1107 4 0.08443057092171102 1107 5 0.09493806886456468 1107 6 0.1403962658440534 1107 7 0.04783025905566518 1107 8 0.07741371581007428 1107 9 0.12071770243951363 1107 10-11 0.21549926165784683 1107 12-13 0.26373903483329286 1107 14-15 0.3672314101444414 1107 16-17 0.3844099352271684 1107 18-19 0.2972597015386711 1107 20-21 0.29910070122442534 1107 22-23 0.19819449188033644 1107 24-25 0.06038946158483327 1107 26-27 0.14141213230560368 1107 28-29 0.13238507303960034 1107 30-31 0.0647238526166447 1107 32-33 0.2926017515043142 1107 34-35 0.3661414978446942 1107 36-37 0.4017498454125743 1107 38-39 0.5801674178969023 1107 40-41 0.4535981855508666 1107 42-43 0.5937805584338776 1107 44-45 0.5166157078182181 1107 46-47 0.4268336381523099 1107 48-49 0.6008651462442387 1107 50-51 0.49184954774730016 1107 52-53 0.555566063496201 1107 54-55 0.6631645375364386 1107 56-57 0.49692218939122057 1107 58-59 0.5563633876124285 1107 60-61 0.3518369034594002 1107 62-63 0.45897678856878343 1107 64-65 0.5962015381454648 1107 66-67 0.3853188685590965 1107 68-69 0.5024625416620196 1107 70-71 0.3538617790399172 1107 72-73 0.41787181296649933 1107 74-75 0.5581124805353923 1107 76-77 0.4784750109705804 1107 78-79 0.578600714489923 1107 80-81 0.5184997444672561 1107 82-83 0.6218142219459182 1107 84-85 0.5614913069680192 1107 86-87 0.5254155641337839 1107 88-89 0.4814983834638795 1107 90-91 0.5319910879196996 1107 92-93 0.5724522650769934 1107 94-95 0.4153720557943963 1107 96-97 0.4772341856849991 1107 98-99 0.726243541516105 1107 100-101 0.6155425896173234 1108 1 0.22075716687647073 1108 2 0.2491800556096635 1108 3 0.2718690078379886 1108 4 0.3162073194672246 1108 5 0.3653113790049858 1108 6 0.2734233627054081 1108 7 0.407092927197219 1108 8 0.36012416190767027 1108 9 0.49942779388825187 1108 10-11 0.4107265957961701 1108 12-13 0.20623325067933962 1108 14-15 0.4561836760973037 1108 16-17 0.5129663455052267 1108 18-19 0.45187404201846704 1108 20-21 0.42513044950818113 1108 22-23 0.5502022688230923 1108 24-25 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2316 84-85 -1.8180426028748933 2316 86-87 -1.6498944427830828 2316 88-89 -1.9738761130291778 2316 90-91 -1.9991948489396698 2316 92-93 -2.022454881513557 2316 94-95 -2.245226706681777 2316 96-97 -2.0114607728294693 2316 98-99 -2.0325809360416436 2316 100-101 -1.566001787849352 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10983.0 3 3712.0 4 3602.0 5 3856.0 6 4209.0 7 4959.0 8 5946.0 9 7006.0 10 8232.0 11 9545.0 12 11289.0 13 13898.0 14 13098.0 15 13227.0 16 14963.0 17 18165.0 18 16743.0 19 14165.0 20 14736.0 21 15564.0 22 17324.0 23 19075.0 24 21619.0 25 24351.0 26 27919.0 27 31948.0 28 37457.0 29 43223.0 30 51743.0 31 62015.0 32 76391.0 33 96452.0 34 129038.0 35 179626.0 36 272815.0 37 416493.0 38 382548.0 39 56649.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.71044618845977 19.06114530982233 15.070740600422425 23.157667901295476 2 15.006648744637097 19.901537249231747 40.4484812891967 24.64333271693446 3 18.945160482388133 23.862302007905974 29.803865443209084 27.38867206649681 4 12.884403149620935 16.861856733604245 37.451074695458416 32.802665421316405 5 14.182129287461615 36.86989278092958 34.31651560327457 14.631462328334239 6 31.94430319317647 35.986519752964305 17.77875918384785 14.290417870011375 7 28.674831074412715 32.13800880284715 20.796482576595313 18.39067754614482 8 26.063204750806523 35.27081845145481 19.85893604747834 18.807040750260327 9 25.91845360010775 17.174346184392025 20.47795534872488 36.42924486677535 10-11 24.914134711729254 26.597723649730465 27.614011988979335 20.87412964956095 12-13 25.582308566704558 23.721438415889406 27.982393094712005 22.71385992269403 14-15 22.58724739408995 25.46339093747743 25.968887213886298 25.98047445454632 16-17 22.413025204229964 28.57461650280279 26.62589807775368 22.386460215213567 18-19 22.329578757385843 27.36409633339 28.62692315194886 21.679401757275304 20-21 23.015145723419742 26.57468566202682 28.50648647920569 21.903682135347747 22-23 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27.58188372093565 22.738347300959866 48-49 22.79822929902183 26.924926890453893 27.59754636094395 22.679297449580325 50-51 22.646233566112052 27.10635704139126 27.56290859815474 22.684500794341947 52-53 22.86393455375805 27.069359619404125 27.364551536074018 22.702154290763804 54-55 22.581291961742767 26.934135055390087 27.56903092389773 22.91554205896942 56-57 22.6347184097116 27.208171228874033 27.516012544615105 22.64109781679926 58-59 22.75863064344878 26.931839688470667 27.591195031509336 22.71833463657122 60-61 22.72541010786121 26.920904802759395 27.795001101811835 22.55868398756756 62-63 22.52175366892322 26.952055304895993 27.773511287131665 22.752679739049118 64-65 22.4931322040815 26.750140803209522 27.727960279752335 23.028766712956646 66-67 22.4755428693354 27.067506292769988 27.483460368942904 22.973490468951706 68-69 22.429700575143226 26.903810743213274 27.670334462746148 22.99615421889735 70-71 22.452646672716025 26.89193738329841 27.59102287380328 23.064393070182284 72-73 22.543697683145243 26.802324890739776 27.595837222243315 23.058140203871666 74-75 22.469199701192085 27.033287918940786 27.35885996442164 23.138652415445492 76-77 22.514116431887707 26.835904703458862 27.445347953015485 23.20463091163795 78-79 22.506811849521345 26.91110158003242 27.47055571932246 23.111530851123774 80-81 22.46982788629072 27.054885503950498 27.392272055103213 23.083014554655573 82-83 22.551938734020865 27.001132226443424 27.394756734325465 23.052172305210245 84-85 22.54676330703065 26.8459506578932 27.35525953583002 23.25202649924613 86-87 22.44591325861337 26.71470392278642 27.59228972697149 23.247093091628717 88-89 22.44970684598986 26.686133406560696 27.512712889809354 23.35144685764009 90-91 22.424820888961055 26.751372002323148 27.605103753125547 23.218703355590247 92-93 22.512089358987918 26.477245354699626 27.689745511946324 23.320919774366132 94-95 22.365885221021344 26.54541388431716 27.6049605316952 23.4837403629663 96-97 22.38692198896809 26.468430551546586 27.6595665373002 23.485080922185126 98-99 22.31398336186488 26.41104202301982 27.502819183914685 23.772155431200616 100-101 22.466467593841134 25.890343198011323 28.006039256565003 23.637149951582543 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1407.0 1 780.0 2 169.5 3 190.5 4 169.0 5 167.5 6 275.0 7 561.0 8 973.5 9 1254.0 10 1280.0 11 1313.5 12 1519.0 13 1739.0 14 1919.5 15 2063.0 16 2310.0 17 2462.0 18 2432.0 19 2586.5 20 2819.0 21 3140.5 22 3727.5 23 4515.0 24 5442.5 25 6939.0 26 9146.0 27 12154.0 28 15294.5 29 18749.5 30 22846.5 31 27226.0 32 31410.5 33 35270.5 34 39283.0 35 43295.5 36 47755.0 37 52675.0 38 57039.0 39 59933.5 40 62905.0 41 67605.5 42 72559.0 43 76966.5 44 80580.5 45 84121.5 46 86206.5 47 87899.5 48 92009.0 49 92106.0 50 88387.5 51 84934.0 52 81194.0 53 78009.5 54 74467.5 55 68549.5 56 60320.0 57 52708.5 58 45945.5 59 38907.0 60 32436.5 61 26818.0 62 22012.5 63 17245.0 64 13264.5 65 10401.0 66 7825.5 67 6215.5 68 4832.0 69 3329.0 70 2472.0 71 1939.5 72 1558.5 73 1247.0 74 936.0 75 735.5 76 526.5 77 308.0 78 172.0 79 107.5 80 78.0 81 56.5 82 42.5 83 30.5 84 20.0 85 14.0 86 10.5 87 9.0 88 5.5 89 6.5 90 5.5 91 5.0 92 5.0 93 3.5 94 3.0 95 2.5 96 2.0 97 2.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.280470083268472 2 0.1072590373048881 3 0.11853724849705072 4 0.12030091938306793 5 0.12721636575192485 6 0.056808767486448745 7 0.06636972018433145 8 0.07105737174979822 9 0.06808697867861135 10-11 0.06356177048422514 12-13 0.06797094769926813 14-15 0.06342253330901323 16-17 0.06437398733962778 18-19 0.06920087608030644 20-21 0.07333157894492566 22-23 0.08159298467416413 24-25 0.08969194703232206 26-27 0.09045775149598742 28-29 0.08384398567342292 30-31 0.08031664390138851 32-33 0.07309951698623919 34-35 0.2394415289727035 36-37 0.36303772816911933 38-39 0.3349350249721874 40-41 0.318180151555024 42-43 0.34120069785672213 44-45 0.35484594102748684 46-47 0.36143650065418265 48-49 0.3708118037851162 50-51 0.3644068937253695 52-53 0.364708574271662 54-55 0.3505063824000498 56-57 0.32776431044877535 58-59 0.3128891388969724 60-61 0.2721390589516276 62-63 0.342198564279074 64-65 0.3705565356305611 66-67 0.3752905995877652 68-69 0.38749705861467365 70-71 0.3882164506866017 72-73 0.37136875248596374 74-75 0.3744319703406252 76-77 0.35828045801604663 78-79 0.33584006661106464 80-81 0.334563725838289 82-83 0.3480929380297104 84-85 0.355890219841576 86-87 0.3487195053181639 88-89 0.3647317804675306 90-91 0.3885877498205001 92-93 0.38466590271869866 94-95 0.3831110875954993 96-97 0.3884021002535509 98-99 0.3779128997209223 100-101 0.3547763224398809 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2154597.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.63922502784632 #Duplication Level Percentage of deduplicated Percentage of total 1 79.40908719964607 49.74123682354501 2 12.251877177586689 15.348961830807747 3 3.8187756122105543 7.176154347123255 4 1.5691693425016484 3.931662062070336 5 0.8253485320966782 2.5849596214203236 6 0.4775058128874641 1.7946356439337525 7 0.3330075846867778 1.4601535923222266 8 0.23194944197031003 1.1623306632529307 9 0.1721155933699834 0.9703068645513315 >10 0.8251849987958794 9.126341271017045 >50 0.05474118120721398 2.3632667370148086 >100 0.029602739640845237 3.2220912734815146 >500 0.0011146250453900556 0.44530717037619433 >1k 5.20158354515128E-4 0.6725920990836964 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4318 0.20040870752163864 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3515 0.16313955695659096 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2185 0.10141107594598897 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.3206195868647362E-5 0.0 16-17 0.0 0.0 0.0 4.6412391737294725E-5 0.0 18-19 0.0 0.0 0.0 9.282478347458945E-5 0.0 20-21 0.0 0.0 0.0 9.282478347458945E-5 0.0 22-23 0.0 0.0 0.0 1.1603097934323681E-4 0.0 24-25 0.0 0.0 0.0 1.3923717521188418E-4 0.0 26-27 0.0 0.0 0.0 2.3206195868647362E-4 0.0 28-29 0.0 0.0 0.0 0.0010442788140891312 0.0 30-31 0.0 0.0 0.0 0.003921847101801404 0.0 32-33 0.0 0.0 0.0 0.010001870419387014 0.0 34-35 0.0 0.0 0.0 0.02005015323051132 0.0 36-37 2.3206195868647362E-5 0.0 0.0 0.037431593936128195 0.0 38-39 4.6412391737294725E-5 0.0 0.0 0.0641883377726786 0.0 40-41 4.6412391737294725E-5 0.0 0.0 0.09314967021675051 0.0 42-43 4.6412391737294725E-5 0.0 0.0 0.125034983340272 0.0 44-45 4.6412391737294725E-5 0.0 0.0 0.16583147567735404 0.0 46-47 4.6412391737294725E-5 0.0 0.0 0.2147965489602 0.0 48-49 4.6412391737294725E-5 0.0 0.0 0.27049141904495366 0.0 50-51 4.6412391737294725E-5 0.0 0.0 0.3319182195092632 0.0 52-53 4.6412391737294725E-5 0.0 0.0 0.40030687873416704 0.0 54-55 4.6412391737294725E-5 0.0 0.0 0.4747523550807877 0.0 56-57 4.6412391737294725E-5 0.0 0.0 0.5663240039784703 0.0 58-59 4.6412391737294725E-5 0.0 0.0 0.6691970702641841 2.3206195868647362E-5 60-61 4.6412391737294725E-5 0.0 0.0 0.7805868104336913 4.6412391737294725E-5 62-63 4.6412391737294725E-5 0.0 0.0 0.8960608410760806 4.6412391737294725E-5 64-65 4.6412391737294725E-5 0.0 0.0 1.027709590238917 4.6412391737294725E-5 66-67 4.6412391737294725E-5 0.0 0.0 1.1766701615197643 4.6412391737294725E-5 68-69 4.6412391737294725E-5 0.0 0.0 1.3488833410609966 4.6412391737294725E-5 70-71 4.6412391737294725E-5 0.0 0.0 1.5409842304616594 4.6412391737294725E-5 72-73 4.6412391737294725E-5 0.0 0.0 1.739884535251836 4.6412391737294725E-5 74-75 4.6412391737294725E-5 0.0 0.0 1.9561894869434981 4.6412391737294725E-5 76-77 4.6412391737294725E-5 0.0 0.0 2.197278655822876 4.6412391737294725E-5 78-79 4.6412391737294725E-5 0.0 0.0 2.4686983227025747 4.6412391737294725E-5 80-81 4.6412391737294725E-5 0.0 0.0 2.756083852339904 4.6412391737294725E-5 82-83 4.6412391737294725E-5 0.0 0.0 3.0670468769797785 4.6412391737294725E-5 84-85 4.6412391737294725E-5 0.0 0.0 3.387617266709273 4.6412391737294725E-5 86-87 6.961858760594209E-5 0.0 0.0 3.734897987883581 4.6412391737294725E-5 88-89 9.282478347458945E-5 0.0 0.0 4.1140408159855415 4.6412391737294725E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4670 0.0 34.285736 1 GTATCAA 7145 0.0 33.248207 1 ATCAACG 8570 0.0 27.560768 3 TCAACGC 8660 0.0 27.27434 4 CAACGCA 8745 0.0 26.955 5 TATCAAC 8870 0.0 26.682083 2 AACGCAG 8960 0.0 26.237585 6 ACGCAGA 10070 0.0 23.204258 7 GTACATG 6675 0.0 22.848318 1 TACATGG 6550 0.0 22.809305 2 CGCAGAG 10250 0.0 22.704285 8 ACATGGG 6760 0.0 21.749926 3 GAGTACT 5130 0.0 20.74241 12-13 GTACCGT 185 6.6808498E-6 20.495968 6 GCAGAGT 10875 0.0 20.483713 9 CATGGGG 4385 0.0 20.118063 4 GTGTAGC 1210 0.0 20.025587 1 AGAGTAC 8830 0.0 19.752964 10-11 GTACTTT 5580 0.0 18.984915 14-15 AGTACTT 5560 0.0 18.754616 12-13 >>END_MODULE