FastQCFastQC Report
Fri 27 May 2016
SRR939282_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939282_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2154597
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40600.18843431045341658No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36750.17056553963455812No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23510.1091155329743799No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51100.035.7665631
GTATCAA75300.031.9200271
ATCAACG91400.025.7513663
TCAACGC92500.025.4964354
TATCAAC94400.025.1843362
CAACGCA93650.025.1833445
AACGCAG96400.024.4649416
TACATGG64450.021.8674582
GTACATG67800.021.7620031
ACGCAGA108550.021.6391477
CGCAGAG110400.021.2765358
ACATGGG66450.020.495183
GAGTACT58200.020.26228312-13
AGAGTAC95850.019.92753810-11
GCAGAGT118500.019.3421029
ATACCGG1250.0060673218.9812496
GTACTTT62300.018.73837914-15
AGTACTT61950.018.65273912-13
CAGAGTA114400.017.214810-11
GGACCGA1952.2937052E-417.0344546
Produced by FastQC (version 0.11.5)