FastQCFastQC Report
Fri 27 May 2016
SRR939281_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939281_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2387755
Sequences flagged as poor quality0
Sequence length101
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70030.2932880467217114No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53810.22535812928880894No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36810.1541615450496387No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA28190.11806068880601234No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA140150.026.1368471
GGTATCA105050.025.2941991
GTACATG122250.025.0733661
TACATGG122850.024.4663522
ACATGGG126150.023.0747073
ATCAACG163950.022.178853
GAGTACT70150.022.01607112-13
TCAACGC165900.021.9753114
TATCAAC166800.021.9704282
CAACGCA167800.021.6982335
AACGCAG172850.021.1063046
CATGGGG50050.021.028294
GTACTTT75150.020.3359514-15
AGTACTT72350.019.93878212-13
CATGGGA78100.019.8496044
ACGCAGA191450.018.932027
CGCAGAG192150.018.8634438
GCAGAGT196500.017.841939
GTATAGG5500.017.2542571
ATGGGAG31950.016.7672825
Produced by FastQC (version 0.11.5)