##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939281_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2387755 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.801934034270687 33.0 31.0 34.0 30.0 34.0 2 31.870795370546812 33.0 31.0 34.0 30.0 34.0 3 31.918729517894423 34.0 31.0 34.0 30.0 34.0 4 35.410679487635875 37.0 35.0 37.0 33.0 37.0 5 35.44580536947886 37.0 35.0 37.0 33.0 37.0 6 35.53148627057634 37.0 35.0 37.0 33.0 37.0 7 35.46436925061407 37.0 35.0 37.0 33.0 37.0 8 35.4444798566017 37.0 35.0 37.0 33.0 37.0 9 37.051634694514306 39.0 37.0 39.0 33.0 39.0 10-11 36.9398571042674 39.0 37.0 39.0 33.0 39.0 12-13 36.83615174086118 39.0 37.0 39.0 33.0 39.0 14-15 38.0461709848791 40.0 38.0 41.0 33.0 41.0 16-17 37.971117220987914 40.0 38.0 41.0 33.0 41.0 18-19 37.89753890160422 40.0 38.0 41.0 33.0 41.0 20-21 37.86814162257015 40.0 38.0 41.0 32.5 41.0 22-23 37.870652977378334 40.0 38.0 41.0 32.0 41.0 24-25 37.78736909775082 40.0 38.0 41.0 32.0 41.0 26-27 37.70167647015711 40.0 38.0 41.0 32.0 41.0 28-29 37.5504649765156 40.0 37.5 41.0 32.0 41.0 30-31 37.376414456257024 40.0 37.0 41.0 31.5 41.0 32-33 37.164373019845 40.0 37.0 41.0 31.0 41.0 34-35 36.883014379616 40.0 36.0 41.0 30.5 41.0 36-37 36.65164076716414 40.0 36.0 41.0 30.0 41.0 38-39 36.057372929802256 38.5 35.5 40.5 28.5 41.0 40-41 36.037227018684916 39.0 35.0 40.0 30.0 41.0 42-43 36.22077013763975 39.0 35.0 41.0 30.0 41.0 44-45 36.126786877213114 39.0 35.0 41.0 29.0 41.0 46-47 35.84688462593524 39.0 35.0 41.0 27.5 41.0 48-49 35.6062761883024 39.0 35.0 41.0 27.0 41.0 50-51 34.47915405893821 38.0 34.0 40.0 25.0 40.5 52-53 34.585135200219455 38.0 34.5 39.5 25.0 40.5 54-55 35.07778373409332 39.0 35.0 40.0 26.0 41.0 56-57 35.00009548718357 39.0 35.0 40.5 26.0 41.0 58-59 34.79458026472565 38.0 34.5 40.0 25.0 41.0 60-61 34.49314439714293 38.0 34.0 40.0 24.5 41.0 62-63 34.107787231102016 37.5 34.0 40.0 23.0 41.0 64-65 33.71263467148012 37.0 33.5 40.0 22.0 41.0 66-67 33.3488456730276 36.5 33.0 39.5 22.0 41.0 68-69 32.94194379239076 36.0 33.0 39.0 20.5 41.0 70-71 32.52154659921139 35.5 32.0 39.0 19.5 40.0 72-73 32.07815814436573 35.0 32.0 38.0 18.0 40.0 74-75 31.613720209988045 35.0 31.0 37.0 16.0 39.5 76-77 31.14281050610301 35.0 31.0 37.0 12.0 39.0 78-79 30.74727641655027 35.0 31.0 36.0 7.5 39.0 80-81 30.339004839273713 34.5 30.5 36.0 7.0 37.5 82-83 29.910207496162712 34.0 30.0 35.0 2.0 37.0 84-85 29.488000862735078 34.0 29.5 35.0 2.0 36.5 86-87 29.046370754118406 34.0 29.0 35.0 2.0 36.0 88-89 28.611867842387515 34.0 29.0 35.0 2.0 36.0 90-91 28.193131414236383 34.0 29.0 35.0 2.0 35.5 92-93 27.735401663906053 34.0 27.0 35.0 2.0 35.0 94-95 27.241379664161524 33.5 26.5 35.0 2.0 35.0 96-97 26.612892235593684 33.0 25.0 35.0 2.0 35.0 98-99 25.796001892991534 33.0 23.5 35.0 2.0 35.0 100-101 23.111973171451844 29.5 9.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.31689501890372185 1101 2 0.4561342838697193 1101 3 0.4978525042125419 1101 4 0.23195177197442973 1101 5 0.3248826780753049 1101 6 0.3504806201839017 1101 7 0.3278378868394469 1101 8 0.19422450538873903 1101 9 0.37675747887836764 1101 10-11 0.5046379906891687 1101 12-13 0.546525661507907 1101 14-15 0.6297041823785392 1101 16-17 0.6245919960004258 1101 18-19 0.7037964454720651 1101 20-21 0.8240889235478122 1101 22-23 0.83884547499418 1101 24-25 0.8342397547370553 1101 26-27 0.8141402213921296 1101 28-29 0.8013650610803822 1101 30-31 0.7556820795934271 1101 32-33 0.7585738245903713 1101 34-35 0.6970049947418033 1101 36-37 0.6483794920705179 1101 38-39 1.1316221251645544 1101 40-41 0.9437376143611615 1101 42-43 0.8511285711819738 1101 44-45 0.7789938390694715 1101 46-47 0.8369387923155145 1101 48-49 0.7444104838384433 1101 50-51 0.6419052943001944 1101 52-53 0.7871671808373506 1101 54-55 0.7858408778165327 1101 56-57 0.7364529399252007 1101 58-59 0.7966804376331922 1101 60-61 0.7085234560703597 1101 62-63 0.8008606723960341 1101 64-65 0.6761127304456096 1101 66-67 0.8568293205894548 1101 68-69 0.7122279021446261 1101 70-71 0.7598770531604231 1101 72-73 0.8401210892622117 1101 74-75 0.7819073411058817 1101 76-77 0.856903166987049 1101 78-79 0.8257448016773878 1101 80-81 0.8068611484622998 1101 82-83 0.6125339294189232 1101 84-85 0.6926072345348473 1101 86-87 0.7158245087467598 1101 88-89 0.8236387336263 1101 90-91 0.8869801639616206 1101 92-93 0.9269280304228928 1101 94-95 0.9164054383009592 1101 96-97 0.9560523692410818 1101 98-99 0.9699729773010262 1101 100-101 0.7466726879665906 1102 1 0.3132765104783246 1102 2 0.28823123246879234 1102 3 0.38874938260274305 1102 4 0.31062720306333347 1102 5 0.2755509604631925 1102 6 0.27772900714395377 1102 7 0.361399014379721 1102 8 0.3690501426329931 1102 9 0.38534408598140146 1102 10-11 0.4314318173677876 1102 12-13 0.4280156828168771 1102 14-15 0.5503924556552633 1102 16-17 0.5217972435588152 1102 18-19 0.4798903913326811 1102 20-21 0.5230444223514468 1102 22-23 0.6121730049267029 1102 24-25 0.5756844908801781 1102 26-27 0.5595811382081521 1102 28-29 0.42168950861267973 1102 30-31 0.3643142203892822 1102 32-33 0.4493147301117375 1102 34-35 0.49378725825895486 1102 36-37 0.47783340063433855 1102 38-39 0.8266292625487139 1102 40-41 0.6260608889348802 1102 42-43 0.48002198483981573 1102 44-45 0.534238035687153 1102 46-47 0.426946166022212 1102 48-49 0.25345264976880344 1102 50-51 0.24904283378990755 1102 52-53 0.28847338981791637 1102 54-55 0.25550305642621396 1102 56-57 0.3569980661003527 1102 58-59 0.3347717460012447 1102 60-61 0.20599347362035303 1102 62-63 0.3155564942359206 1102 64-65 0.20030783033681132 1102 66-67 0.3201521751081984 1102 68-69 0.16853952213445922 1102 70-71 0.0588513468167946 1102 72-73 0.062189375156108895 1102 74-75 -0.16717458470304436 1102 76-77 -0.1995881246941984 1102 78-79 -0.06513021965265864 1102 80-81 0.06002415353513513 1102 82-83 -0.14509812328299887 1102 84-85 -0.06712109884989204 1102 86-87 -0.2645089079466665 1102 88-89 -0.19519672664677046 1102 90-91 -0.12214909736072954 1102 92-93 -0.2283621658756978 1102 94-95 -0.30394146541902245 1102 96-97 -0.1393404953274704 1102 98-99 -0.08601549104717421 1102 100-101 -0.3778787152899561 1103 1 0.45126432385310267 1103 2 0.5142842241323002 1103 3 0.39560846084592427 1103 4 0.40342845215193535 1103 5 0.3826496973547293 1103 6 0.47614582824925833 1103 7 0.4057946710354656 1103 8 0.49186198971338513 1103 9 0.5857758891613116 1103 10-11 0.6219316786606583 1103 12-13 0.6249140763283663 1103 14-15 0.7561713015181866 1103 16-17 0.7301984212270938 1103 18-19 0.8396163605887068 1103 20-21 0.8377859221185631 1103 22-23 0.8630675700361721 1103 24-25 0.7840502992955862 1103 26-27 0.7548535048927008 1103 28-29 0.7436186371526361 1103 30-31 0.7323593149082868 1103 32-33 0.8069527415756284 1103 34-35 0.8241651638111165 1103 36-37 0.4921091555228614 1103 38-39 1.1618065200338918 1103 40-41 1.0732405319270057 1103 42-43 0.8811574656975907 1103 44-45 0.8959817568990331 1103 46-47 0.9049605532115521 1103 48-49 0.8217204214334402 1103 50-51 0.6641978555206904 1103 52-53 0.7958539514731271 1103 54-55 0.8405091139434404 1103 56-57 0.8182376263113937 1103 58-59 0.9028154768864027 1103 60-61 0.9736474182934671 1103 62-63 0.8028765105483942 1103 64-65 0.8798798708997992 1103 66-67 0.9342787399313011 1103 68-69 0.8658140868263118 1103 70-71 0.7415369469712303 1103 72-73 0.8137355015624053 1103 74-75 0.7408309473890107 1103 76-77 0.6681360897355226 1103 78-79 0.799782671843424 1103 80-81 0.737533271771138 1103 82-83 0.6250726846402301 1103 84-85 0.7025864997014182 1103 86-87 0.7921339917184334 1103 88-89 0.7069943985684795 1103 90-91 0.6336088465521605 1103 92-93 0.7534281021252305 1103 94-95 0.5132587300919766 1103 96-97 0.6193516050990517 1103 98-99 0.6069523344383754 1103 100-101 0.0624193708073264 1104 1 0.2458845192163821 1104 2 0.3732516533918293 1104 3 0.34026531058612264 1104 4 0.15025910893059802 1104 5 0.31727721532860187 1104 6 0.18539727727488042 1104 7 0.28976022005356583 1104 8 0.34832270589114955 1104 9 0.4853098372302824 1104 10-11 0.422288263348797 1104 12-13 0.4375486375626636 1104 14-15 0.46130646814889786 1104 16-17 0.5222645875429279 1104 18-19 0.47578695757982103 1104 20-21 0.5712999239252454 1104 22-23 0.5975865712188693 1104 24-25 0.5244160248497636 1104 26-27 0.4719625035215742 1104 28-29 0.5444037515911546 1104 30-31 0.6040082494519154 1104 32-33 0.6346548551322968 1104 34-35 0.5461846918746502 1104 36-37 0.6435834714249111 1104 38-39 1.1728958120796023 1104 40-41 0.8220030851213167 1104 42-43 0.8215387290710652 1104 44-45 0.881801430588304 1104 46-47 0.9753032778342856 1104 48-49 0.8855911897538036 1104 50-51 0.7171066096768541 1104 52-53 0.6486924135265539 1104 54-55 0.7084963299546629 1104 56-57 0.8256598277063247 1104 58-59 0.7713909398596925 1104 60-61 0.7767336589759353 1104 62-63 0.8047090227740981 1104 64-65 0.7607388891575653 1104 66-67 0.8469652867418702 1104 68-69 0.5794471182465912 1104 70-71 0.6987767442853325 1104 72-73 0.6710010023967357 1104 74-75 0.595620521512263 1104 76-77 0.7462533027466272 1104 78-79 0.5326666894606618 1104 80-81 0.749787900264355 1104 82-83 0.5740464447346447 1104 84-85 0.6907184571112666 1104 86-87 0.6394476432630007 1104 88-89 0.33982767032907546 1104 90-91 0.4633073219449635 1104 92-93 0.5248802105163932 1104 94-95 0.7248239091777435 1104 96-97 0.6498571792917254 1104 98-99 0.7428121750434897 1104 100-101 0.4658191779069547 1105 1 0.27267832771101297 1105 2 0.3661897784509982 1105 3 0.3785289999133603 1105 4 0.2660249202299241 1105 5 0.2712171839763613 1105 6 0.3288586349198326 1105 7 0.2793765963831092 1105 8 0.31905473411137564 1105 9 0.44747816376848704 1105 10-11 0.5538066085796132 1105 12-13 0.45346984621228614 1105 14-15 0.5771426775767168 1105 16-17 0.4954368836530918 1105 18-19 0.5805576241438004 1105 20-21 0.579126952313942 1105 22-23 0.6191392290189839 1105 24-25 0.630919768987269 1105 26-27 0.4661868485181344 1105 28-29 0.5599870091111185 1105 30-31 0.6120984184234715 1105 32-33 0.5569074428150742 1105 34-35 0.57421879104335 1105 36-37 0.4864321848439559 1105 38-39 0.77101756594368 1105 40-41 0.6498419548362406 1105 42-43 0.5689472355717626 1105 44-45 0.4492029253551735 1105 46-47 0.47283292527404086 1105 48-49 0.36855263644938674 1105 50-51 0.2815100667664794 1105 52-53 0.3251480302141587 1105 54-55 0.5439753019735178 1105 56-57 0.5018928178337418 1105 58-59 0.43695530420671247 1105 60-61 0.6371556305727992 1105 62-63 0.6251737386705116 1105 64-65 0.5263789064221527 1105 66-67 0.5464236423378637 1105 68-69 0.3946283643456141 1105 70-71 0.4088914912291344 1105 72-73 0.46893189911245514 1105 74-75 0.5058853709043838 1105 76-77 0.5015349674638081 1105 78-79 0.36780069059345166 1105 80-81 0.23126416706965358 1105 82-83 0.12337327015210064 1105 84-85 0.28985486662219273 1105 86-87 0.33030418992793287 1105 88-89 0.2258526991702432 1105 90-91 0.23616625612933362 1105 92-93 0.38973024445190063 1105 94-95 0.10572812600558024 1105 96-97 0.2895272667859672 1105 98-99 0.5894343083755373 1105 100-101 0.48144996920598615 1106 1 0.2503299145475282 1106 2 0.2949223613269787 1106 3 0.32826036597527164 1106 4 0.3234868938400268 1106 5 0.2444392939669271 1106 6 0.2613968969201963 1106 7 0.25205756232523413 1106 8 0.2438915738605303 1106 9 0.31859562622852167 1106 10-11 0.4364208169550352 1106 12-13 0.42569821204086367 1106 14-15 0.5114063112525926 1106 16-17 0.5256521214182612 1106 18-19 0.5130260580409995 1106 20-21 0.539861232345352 1106 22-23 0.5143602126394882 1106 24-25 0.5425185304362259 1106 26-27 0.5458107420039582 1106 28-29 0.6404670806455002 1106 30-31 0.4555154048743475 1106 32-33 0.4104952012550598 1106 34-35 0.436499910816309 1106 36-37 0.36956169611943324 1106 38-39 0.8560009326044948 1106 40-41 0.8103547230236572 1106 42-43 0.6300895777374507 1106 44-45 0.5207497498960549 1106 46-47 0.3905198422806322 1106 48-49 0.34149381895554143 1106 50-51 0.34708093573590304 1106 52-53 0.4024365299150858 1106 54-55 0.3137238295980822 1106 56-57 0.5493083799813192 1106 58-59 0.5214265981110131 1106 60-61 0.5531445371198132 1106 62-63 0.2763488268914287 1106 64-65 0.304325158353592 1106 66-67 0.11732614824523324 1106 68-69 0.18181007170068852 1106 70-71 0.24283845683228122 1106 72-73 0.15263700296726768 1106 74-75 0.31314774064410145 1106 76-77 0.4934719591437151 1106 78-79 0.5248099880866306 1106 80-81 0.5589449392031689 1106 82-83 0.5273697937576074 1106 84-85 0.5607203588846659 1106 86-87 0.5291566719322347 1106 88-89 0.5751483568692741 1106 90-91 0.49322459480190517 1106 92-93 0.5736606983212731 1106 94-95 0.6391252451735596 1106 96-97 0.8085126254953074 1106 98-99 0.6394626766362279 1106 100-101 0.3111693419349102 1107 1 0.11337548579271584 1107 2 0.08180404724910417 1107 3 0.19859412720761682 1107 4 0.18353824172192645 1107 5 0.12206605905036838 1107 6 0.1263615952513959 1107 7 0.10441582077623934 1107 8 0.1844462379598042 1107 9 0.2679125924245014 1107 10-11 0.339770196501469 1107 12-13 0.3846314063070153 1107 14-15 0.3338234058182721 1107 16-17 0.3143767149141965 1107 18-19 0.38804023219590533 1107 20-21 0.3596609221185645 1107 22-23 0.29247478260097637 1107 24-25 0.44698659847216504 1107 26-27 0.4520912298088362 1107 28-29 0.38921066216025935 1107 30-31 0.3130405439415327 1107 32-33 0.4792139111395244 1107 34-35 0.46245332331096023 1107 36-37 0.2870782772063052 1107 38-39 0.9022538744102278 1107 40-41 0.6549474166479641 1107 42-43 0.29788188625263956 1107 44-45 0.43439571694783297 1107 46-47 0.47685194540429165 1107 48-49 0.32677569743221113 1107 50-51 0.35845315439230063 1107 52-53 0.4041344866972807 1107 54-55 0.593438741878785 1107 56-57 0.3764609794678435 1107 58-59 0.37713958484614096 1107 60-61 0.38181778273384737 1107 62-63 0.5906925367758973 1107 64-65 0.4897977193292036 1107 66-67 0.3317078341673536 1107 68-69 0.467041404606114 1107 70-71 0.3610813964985198 1107 72-73 0.44221712705798666 1107 74-75 0.49463870127739185 1107 76-77 0.5646780994945964 1107 78-79 0.6109808267687029 1107 80-81 0.5941730628659805 1107 82-83 0.5336585721059208 1107 84-85 0.5160086558465835 1107 86-87 0.7411193912304803 1107 88-89 0.6321859584952882 1107 90-91 0.6523859127308747 1107 92-93 0.5186352918172084 1107 94-95 0.6821387239925585 1107 96-97 0.6627506978102424 1107 98-99 0.8254471118095239 1107 100-101 0.7478838798039718 1108 1 0.04580092075543263 1108 2 0.14743439798094826 1108 3 0.09295756272267042 1108 4 -0.015583547838964762 1108 5 0.153044034747694 1108 6 0.1406691069884829 1108 7 0.13041796106897863 1108 8 0.158723716556878 1108 9 0.16817529609751602 1108 10-11 0.2628004367665824 1108 12-13 0.31812370815733715 1108 14-15 0.375040265801708 1108 16-17 0.34788489635578657 1108 18-19 0.35150871465931033 1108 20-21 0.321630164040684 1108 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-1.2811555938203192 2316 90-91 -1.2518565115115514 2316 92-93 -1.5974070895435695 2316 94-95 -1.519595123920535 2316 96-97 -1.4145288190565388 2316 98-99 -1.5175135085449654 2316 100-101 -0.8715097022429141 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9559.0 3 2409.0 4 2474.0 5 2601.0 6 3152.0 7 3630.0 8 4586.0 9 5603.0 10 6463.0 11 7921.0 12 9943.0 13 12487.0 14 12638.0 15 13231.0 16 16670.0 17 20752.0 18 18452.0 19 13554.0 20 14060.0 21 15026.0 22 16282.0 23 18359.0 24 20833.0 25 23966.0 26 27577.0 27 32252.0 28 37746.0 29 45174.0 30 54458.0 31 66317.0 32 82630.0 33 106822.0 34 145005.0 35 207671.0 36 319471.0 37 488945.0 38 438390.0 39 60640.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.379038782562084 18.98365374194638 14.654671961676986 22.98263551381455 2 14.991851935144423 20.25608083954438 40.787022507455774 23.96504471785542 3 19.21803686016449 24.827661150984355 29.71486783928411 26.239434149567042 4 12.710191108195884 16.430252160086216 37.31161014079116 33.547946590926735 5 13.91491615684747 37.678716531572974 33.616839814920525 14.789527496659034 6 31.640350020271523 35.735156191368226 17.923633998974474 14.70085978938577 7 28.74105279401916 32.956833256244124 20.49312214663333 17.80899180310339 8 26.26284273505277 34.929429438245414 19.885741701762523 18.921986124939295 9 27.008588088404846 17.097287351809655 20.294596135045666 35.59952842473984 10-11 25.08118099981947 26.67355938976965 27.20533765991142 21.03992195049946 12-13 25.97069435796156 24.025909223373233 27.752661052093096 22.250735366572112 14-15 22.902494949187833 25.254836670311708 26.369129184415712 25.473539196084744 16-17 22.13203196620325 28.753923083970083 26.82675623453538 22.287288715291282 18-19 22.049918932271332 27.298184133204384 29.168781081903987 21.483115852620298 20-21 23.212305472210016 26.395267255148518 28.69284246801611 21.699584804625356 22-23 22.689641631989705 26.433033641024522 28.636214706164914 22.241110020820862 24-25 22.37764115794054 26.60135378686438 28.879863270128357 22.14114178506672 26-27 22.368903526252858 26.660002936795955 28.78831994294225 22.182773594008935 28-29 21.999683676003677 26.9096697812414 29.094423971485533 21.996222571269392 30-31 22.23389301398244 26.625023752904358 28.961197926607408 22.1798853065058 32-33 22.09349058012723 26.70593081968919 28.68756932546428 22.513009274719302 34-35 22.578111832767398 26.820311787458984 28.43592663063009 22.165649749143533 36-37 22.71609125724579 26.456012272894743 28.634173346011345 22.193723123848127 38-39 22.378667171486114 26.605688684156558 28.748941719646414 22.266702424710914 40-41 22.418450599243187 26.651998963544006 28.71670847824368 22.21284195896913 42-43 22.302263003915602 26.656862062429564 28.37551479957396 22.665360134080874 44-45 22.36748099658431 26.877305213411322 28.2008332639087 22.55438052609567 46-47 22.533883169782868 26.97687501669938 27.763970620876044 22.725271192641713 48-49 22.699594074480558 26.83402923370722 27.888147866976283 22.578228824835943 50-51 22.676213571602 27.123237464532586 27.68276318264389 22.51778578122153 52-53 22.612585505648475 27.259589993708722 27.542718632498776 22.58510586814403 54-55 22.291575285765827 26.983972710375927 27.808004914320218 22.916447089538032 56-57 22.456564776303708 27.35822908697391 27.573717594183428 22.61148854253895 58-59 22.572605976191287 26.85326651720511 27.794844880016516 22.779282626587083 60-61 22.555912154706583 26.866145009048093 28.085672070299772 22.492270765945552 62-63 22.571544173357687 26.811309547730446 27.894468964684332 22.722677314227536 64-65 22.326341965397262 26.826368370545655 27.955456723855377 22.891832940201702 66-67 22.279122127767025 26.98379766012962 27.780090059759278 22.95699015234408 68-69 22.216729652343474 27.010270053456804 27.8763494149887 22.896650879211023 70-71 22.24239059941324 27.000741128240435 27.79975817587339 22.957110096472928 72-73 22.32640729276323 26.802764917422174 27.69049943114713 23.180328358667467 74-75 22.167839392868785 27.190547263576903 27.46390865760849 23.177704685945823 76-77 22.22395429023429 26.88882016716209 27.610840081035537 23.276385461568076 78-79 22.260067734890328 26.88424342407632 27.72999352351632 23.125695317517025 80-81 22.162872078600486 27.200132260024724 27.62293104452817 23.01406461684662 82-83 22.274303663740785 27.02078774571036 27.51958172913284 23.185326861416012 84-85 22.230456901272273 26.75792224392036 27.529336131768517 23.482284723038855 86-87 22.264719339858317 26.545416296327463 27.771559000951925 23.418305362862295 88-89 22.257571604062708 26.43361180614186 27.781591593177378 23.52722499661806 90-91 22.061108506506788 26.605275628394693 27.96633102341871 23.367284841679805 92-93 22.131730377699753 26.411689258647048 27.92345627312815 23.533124090525046 94-95 21.986889253076303 26.44459371078076 27.864714195649448 23.703802840493488 96-97 21.995267879914245 26.32403827781804 27.892805106851476 23.78788873541624 98-99 22.026354501566274 26.2507635684777 27.694320944322996 24.028560985633025 100-101 22.14279372776001 25.82788409307124 28.220767127921835 23.808555051246913 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2856.0 1 1663.0 2 478.5 3 495.5 4 454.5 5 471.5 6 729.0 7 1360.5 8 2101.0 9 2400.5 10 2290.5 11 2247.0 12 2399.5 13 2627.0 14 2868.0 15 3038.0 16 3221.5 17 3420.0 18 3556.5 19 3696.0 20 3885.5 21 4324.0 22 5031.0 23 6206.5 24 7509.5 25 8957.5 26 11508.5 27 14677.0 28 17816.5 29 21615.5 30 25779.0 31 30320.5 32 34776.5 33 39015.0 34 44491.0 35 48962.0 36 53797.5 37 59330.0 38 63061.0 39 64815.0 40 67107.5 41 73310.5 42 78912.0 43 82217.0 44 86200.5 45 90391.5 46 92592.0 47 95757.0 48 106972.5 49 106780.0 50 94451.5 51 87974.0 52 84189.0 53 81864.5 54 78495.0 55 72323.5 56 63782.0 57 56848.0 58 51400.0 59 44648.0 60 37663.5 61 31141.0 62 25451.5 63 20506.0 64 15977.0 65 12433.0 66 9816.5 67 8106.5 68 6342.0 69 4320.0 70 3114.5 71 2367.0 72 1873.0 73 1576.0 74 1194.5 75 903.5 76 647.5 77 408.0 78 256.5 79 178.5 80 116.0 81 79.0 82 68.0 83 47.0 84 25.0 85 20.0 86 17.0 87 11.0 88 9.5 89 8.5 90 6.5 91 6.0 92 4.5 93 3.5 94 5.0 95 4.5 96 3.0 97 2.0 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.299402576897546 2 0.18289146080732738 3 0.1829752214946676 4 0.20370599161136718 5 0.21823847086489193 6 0.11039658591438402 7 0.1286564157545477 8 0.13958718545244383 9 0.14264445054036112 10-11 0.13043633036052693 12-13 0.12103419320658945 14-15 0.13558761263194927 16-17 0.11196709880201276 18-19 0.1394196640777634 20-21 0.16490385320101938 22-23 0.17120684492336946 24-25 0.19524616219000693 26-27 0.17610684513277114 28-29 0.17267265695182296 30-31 0.16065299831850421 32-33 0.17049487908097774 34-35 0.3562342032578719 36-37 0.4800115589748529 38-39 0.44675856610079345 40-41 0.4365188220734539 42-43 0.4598671136695348 44-45 0.46390856683369947 46-47 0.4677824986231837 48-49 0.4798230974283375 50-51 0.4723265159113896 52-53 0.4795299350226468 54-55 0.4618354898220295 56-57 0.42441540275279493 58-59 0.41402907752261014 60-61 0.36854702429688135 62-63 0.4763260887318842 64-65 0.47427395189204924 66-67 0.4839273711080073 68-69 0.49494190149324363 70-71 0.48782224306932664 72-73 0.48084916584825493 74-75 0.48566540537031644 76-77 0.4615213872445037 78-79 0.447847455036216 80-81 0.4447483096046286 82-83 0.4574171135648339 84-85 0.4586107033594317 86-87 0.460369677793576 88-89 0.4674474558738229 90-91 0.4866914737902339 92-93 0.4911517303910996 94-95 0.4895393371598007 96-97 0.48803164478767713 98-99 0.48482779849691443 100-101 0.46587694298619414 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2387755.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.146752149859466 #Duplication Level Percentage of deduplicated Percentage of total 1 72.77499577203564 37.22204671459377 2 14.347416831719412 14.676475453653492 3 5.339272837306716 8.192593934106105 4 2.5981267240262196 5.315429744307815 5 1.3784170732433663 3.5250778202156567 6 0.8461761418256633 2.5967496840648914 7 0.5438150889034644 1.94700628972495 8 0.369447577656657 1.5116834949416793 9 0.2727187578601618 1.2553810843402107 >10 1.3693971918625347 12.530464450113666 >50 0.10002635217175278 3.542358150248571 >100 0.05641140739703006 5.2633324603617275 >500 0.002464072168226008 0.8480510820985254 >1k 0.001149900345171512 1.0531007344348806 >5k 1.6427147788164456E-4 0.5202489027940627 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7003 0.2932880467217114 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5381 0.22535812928880894 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3681 0.1541615450496387 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2819 0.11806068880601234 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 6.282051550515023E-5 0.0 12-13 0.0 0.0 0.0 8.376068734020031E-5 0.0 14-15 0.0 0.0 0.0 8.376068734020031E-5 0.0 16-17 0.0 0.0 0.0 8.376068734020031E-5 0.0 18-19 0.0 0.0 0.0 1.884615465154507E-4 0.0 20-21 0.0 0.0 0.0 2.3034189018555085E-4 0.0 22-23 0.0 0.0 0.0 4.8162395220615185E-4 0.0 24-25 0.0 0.0 0.0 8.585470452370532E-4 0.0 26-27 0.0 0.0 0.0 0.0012564103101030048 0.0 28-29 0.0 0.0 0.0 0.003224786462597712 0.0 30-31 0.0 0.0 0.0 0.009464957669442636 0.0 32-33 0.0 0.0 0.0 0.021526496646431484 0.0 34-35 0.0 0.0 0.0 0.04121025817137855 0.0 36-37 0.0 0.0 0.0 0.07331154159451032 0.0 38-39 0.0 0.0 0.0 0.12151581715879561 0.0 40-41 0.0 0.0 0.0 0.17857778540930708 0.0 42-43 0.0 0.0 0.0 0.23656112122056072 0.0 44-45 0.0 0.0 0.0 0.3118829193112359 0.0 46-47 0.0 0.0 0.0 0.4035589916050851 0.0 48-49 0.0 0.0 0.0 0.5051397651769131 0.0 50-51 0.0 0.0 0.0 0.6131491715021014 0.0 52-53 0.0 0.0 0.0 0.7236295181038255 0.0 54-55 0.0 0.0 0.0 0.8491030277394456 0.0 56-57 0.0 0.0 0.0 0.9955376493819509 0.0 58-59 0.0 0.0 0.0 1.1617397932367433 0.0 60-61 0.0 0.0 0.0 1.3519603141863383 0.0 62-63 0.0 0.0 0.0 1.548776989263974 0.0 64-65 0.0 0.0 0.0 1.751645374001939 0.0 66-67 0.0 0.0 0.0 1.9901329910313246 0.0 68-69 0.0 0.0 0.0 2.2574133443339037 0.0 70-71 0.0 0.0 0.0 2.551936861193883 0.0 72-73 0.0 0.0 0.0 2.866918925936706 0.0 74-75 0.0 0.0 0.0 3.193041162095776 0.0 76-77 0.0 0.0 0.0 3.5493800662128234 0.0 78-79 0.0 0.0 0.0 3.9486253824198885 0.0 80-81 0.0 0.0 0.0 4.390190785905589 0.0 82-83 0.0 0.0 0.0 4.852089096243124 0.0 84-85 0.0 4.1880343670100155E-5 0.0 5.340665185498512 0.0 86-87 0.0 4.1880343670100155E-5 0.0 5.860463070959961 0.0 88-89 0.0 4.1880343670100155E-5 0.0 6.4107707867850765 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 14015 0.0 26.136847 1 GGTATCA 10505 0.0 25.294199 1 GTACATG 12225 0.0 25.073366 1 TACATGG 12285 0.0 24.466352 2 ACATGGG 12615 0.0 23.074707 3 ATCAACG 16395 0.0 22.17885 3 GAGTACT 7015 0.0 22.016071 12-13 TCAACGC 16590 0.0 21.975311 4 TATCAAC 16680 0.0 21.970428 2 CAACGCA 16780 0.0 21.698233 5 AACGCAG 17285 0.0 21.106304 6 CATGGGG 5005 0.0 21.02829 4 GTACTTT 7515 0.0 20.33595 14-15 AGTACTT 7235 0.0 19.938782 12-13 CATGGGA 7810 0.0 19.849604 4 ACGCAGA 19145 0.0 18.93202 7 CGCAGAG 19215 0.0 18.863443 8 GCAGAGT 19650 0.0 17.84193 9 GTATAGG 550 0.0 17.254257 1 ATGGGAG 3195 0.0 16.767282 5 >>END_MODULE