FastQCFastQC Report
Fri 27 May 2016
SRR939281_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939281_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2387755
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72440.3033812095462055No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56670.2373359075784576No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39950.1673119729620501No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA29330.12283504798440376No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA24120.10101538893228158No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT23890.10005214102786929No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA153250.025.4334161
GTACATG125700.025.2529351
TACATGG125650.024.7357462
ACATGGG128450.023.2730143
GGTATCA114750.022.3126351
GAGTACT76550.021.26289712-13
ATCAACG183200.021.2131273
TCAACGC184200.021.1237224
TATCAAC185050.021.103622
AGTACTT79350.020.99102812-13
CAACGCA185450.020.9557555
CATGGGA80400.020.9516644
TCGCGCT4350.020.72663360-61
AACGCAG190350.020.416316
GTACTTT81400.019.8794114-15
CATGGGG50350.019.6966824
CGATCGT4900.019.36779424-25
CGCGGAA4500.019.08675282-83
ATTCGCG4400.018.89336480-81
ACGCAGA207500.018.5916827
Produced by FastQC (version 0.11.5)