FastQCFastQC Report
Fri 27 May 2016
SRR939280_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939280_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2430806
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73060.3005587447126591No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53570.22037957780258896No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38000.156326749234616No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA29440.12111209203860776No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA156500.027.254111
GTACATG128950.025.0102731
TACATGG128100.024.7650812
ACATGGG130850.022.9037273
ATCAACG184600.022.8880523
TCAACGC184950.022.8447384
TATCAAC186500.022.8074322
TAGCGCG1252.2827672E-422.7616654
GAGTACT72700.022.75068512-13
CAACGCA186350.022.6476655
AACGCAG191400.022.2098186
GGTATCA114550.022.183431
AGTACTT75850.021.11857212-13
GTACTTT78800.021.01981414-15
CATGGGG49400.020.5423264
ACGCAGA210100.019.9623537
CGCAGAG210350.019.938638
ATACCGT2651.9070285E-819.6716756
CATGGGA84050.019.464544
GCAGAGT214650.019.163489
Produced by FastQC (version 0.11.5)