##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939280_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2430806 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.73742248455862 33.0 31.0 34.0 30.0 34.0 2 31.821499535545 33.0 31.0 34.0 30.0 34.0 3 31.873908078225906 34.0 31.0 34.0 30.0 34.0 4 35.379231004037344 37.0 35.0 37.0 33.0 37.0 5 35.4250067673027 37.0 35.0 37.0 33.0 37.0 6 35.51340172765741 37.0 35.0 37.0 33.0 37.0 7 35.4294986930261 37.0 35.0 37.0 33.0 37.0 8 35.40618255837776 37.0 35.0 37.0 33.0 37.0 9 37.009210936619375 39.0 37.0 39.0 33.0 39.0 10-11 36.90913713393829 39.0 37.0 39.0 33.0 39.0 12-13 36.803041871708395 39.0 37.0 39.0 33.0 39.0 14-15 38.008761291522234 40.0 38.0 41.0 33.0 41.0 16-17 37.94856808811563 40.0 38.0 41.0 33.0 41.0 18-19 37.89144896795548 40.0 38.0 41.0 32.5 41.0 20-21 37.7908829417074 40.0 38.0 41.0 32.0 41.0 22-23 37.70812767452442 40.0 38.0 41.0 32.0 41.0 24-25 37.730804309352536 40.0 38.0 41.0 32.0 41.0 26-27 37.64469233661592 40.0 37.5 41.0 32.0 41.0 28-29 37.50577791892895 40.0 37.0 41.0 32.0 41.0 30-31 37.33841450119837 40.0 37.0 41.0 31.0 41.0 32-33 37.15640470691614 40.0 36.5 41.0 31.0 41.0 34-35 36.858091513679 40.0 36.0 41.0 30.0 41.0 36-37 36.614745479482934 39.5 36.0 41.0 30.0 41.0 38-39 36.30246901644969 39.0 35.5 41.0 29.5 41.0 40-41 36.16939011175717 39.0 35.0 41.0 29.5 41.0 42-43 36.00933023861221 39.0 35.0 41.0 29.0 41.0 44-45 35.98093471877229 39.0 35.0 41.0 28.5 41.0 46-47 35.792844636717206 39.0 35.0 41.0 27.5 41.0 48-49 35.59529431801633 39.0 35.0 41.0 26.5 41.0 50-51 34.46604459590769 38.0 34.0 40.0 25.0 40.5 52-53 34.582491980026376 38.0 34.0 39.5 25.0 40.5 54-55 35.090296798675006 39.0 35.0 40.0 26.0 41.0 56-57 35.001845272720246 39.0 35.0 41.0 26.0 41.0 58-59 34.80745481128481 38.0 34.0 40.0 25.0 41.0 60-61 34.52374294781237 38.0 34.0 40.0 24.5 41.0 62-63 34.16290954522903 37.5 34.0 40.0 23.0 41.0 64-65 33.787360036136164 37.0 33.5 40.0 22.0 41.0 66-67 33.43083857782151 36.5 33.0 39.5 22.0 41.0 68-69 33.020750113336895 36.0 33.0 39.0 21.0 41.0 70-71 32.608974142732905 35.5 32.0 39.0 20.0 40.5 72-73 32.16819894306662 35.0 32.0 38.0 18.5 40.0 74-75 31.716018267192034 35.0 31.5 37.0 17.5 39.5 76-77 31.26419611437523 35.0 31.0 37.0 13.0 39.0 78-79 30.836910267623168 35.0 31.0 36.0 9.5 39.0 80-81 30.375978173494715 34.5 30.5 36.0 7.0 37.5 82-83 29.935126455998546 34.0 30.0 35.0 2.0 37.0 84-85 29.519025171074944 34.0 29.5 35.0 2.0 36.5 86-87 29.182569896569284 34.0 29.0 35.0 2.0 36.0 88-89 28.774099208246156 34.0 29.0 35.0 2.0 36.0 90-91 28.36935629581299 34.0 29.0 35.0 2.0 35.5 92-93 27.95333543688801 34.0 28.0 35.0 2.0 35.0 94-95 27.47628564352729 34.0 27.0 35.0 2.0 35.0 96-97 26.893661608536426 33.5 25.5 35.0 2.0 35.0 98-99 26.14292563865648 33.0 24.5 35.0 2.0 35.0 100-101 23.721279279383054 31.0 11.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5906335411834966 1101 2 0.343250860649988 1101 3 0.5686618039881175 1101 4 0.5467565153642795 1101 5 0.5390580246555885 1101 6 0.544313741947164 1101 7 0.6806836547178534 1101 8 0.641873661669834 1101 9 0.6819949410080781 1101 10-11 0.6305086668408677 1101 12-13 0.6454512337512384 1101 14-15 0.7414583179223797 1101 16-17 0.8433884320491174 1101 18-19 0.9565641317306017 1101 20-21 0.9164258208925276 1101 22-23 0.8641606908786059 1101 24-25 0.942405721357467 1101 26-27 0.8433496571008376 1101 28-29 0.861342621108804 1101 30-31 0.9301382193609697 1101 32-33 1.0494692561318004 1101 34-35 0.9393561754155115 1101 36-37 0.9564912234831837 1101 38-39 0.9832481590062017 1101 40-41 0.8606139494642306 1101 42-43 0.8294174835570729 1101 44-45 0.9979629778711683 1101 46-47 0.9950332906394976 1101 48-49 0.9207274077994256 1101 50-51 0.9242753758188726 1101 52-53 0.8985747575579808 1101 54-55 0.9631170809407195 1101 56-57 0.8826737446855191 1101 58-59 0.9363880462517784 1101 60-61 1.0547403908210953 1101 62-63 1.08666669068986 1101 64-65 0.8980724317977575 1101 66-67 1.0006369622383673 1101 68-69 0.9393540303511756 1101 70-71 0.8903824768481385 1101 72-73 0.9246350193118289 1101 74-75 1.0445818589264029 1101 76-77 0.997022981738926 1101 78-79 0.9604349607133713 1101 80-81 0.8825917406561175 1101 82-83 0.8372598134039109 1101 84-85 0.7854920249090078 1101 86-87 0.6943835333736317 1101 88-89 0.6341041448204763 1101 90-91 0.7811391535176497 1101 92-93 0.7921458923076656 1101 94-95 0.7014549171834581 1101 96-97 0.5180797380473372 1101 98-99 0.36509186831251483 1101 100-101 0.018880951661774503 1102 1 0.2611942888470473 1102 2 0.31147515971540685 1102 3 0.3976337666049439 1102 4 0.40610230975680395 1102 5 0.3128898003565226 1102 6 0.2896408447508989 1102 7 0.23722571079262167 1102 8 0.3717802037259048 1102 9 0.46517251110153524 1102 10-11 0.4097142743174942 1102 12-13 0.47115216833067564 1102 14-15 0.5538414954924775 1102 16-17 0.5513323572827602 1102 18-19 0.5469846924782686 1102 20-21 0.5521734844439337 1102 22-23 0.5478055506916917 1102 24-25 0.5725459082733622 1102 26-27 0.5435833019606449 1102 28-29 0.5526977612957182 1102 30-31 0.5801382193609683 1102 32-33 0.5985346766925446 1102 34-35 0.5131879511164428 1102 36-37 0.4892015038570179 1102 38-39 0.6839490935856389 1102 40-41 0.5736980616137615 1102 42-43 0.5420343059869808 1102 44-45 0.5479629778711725 1102 46-47 0.5588183373684714 1102 48-49 0.5202601180798112 1102 50-51 0.3665650954450328 1102 52-53 0.4179672809224684 1102 54-55 0.38694885664165923 1102 56-57 0.4469260811341087 1102 58-59 0.36816374718635814 1102 60-61 0.3108151571762292 1102 62-63 0.3324610832132251 1102 64-65 0.44760514207813173 1102 66-67 0.4973659342009782 1102 68-69 0.33444748829509763 1102 70-71 0.32215817778271827 1102 72-73 0.2802424959473413 1102 74-75 0.1796285878983639 1102 76-77 0.24865849575762056 1102 78-79 0.31954711024608073 1102 80-81 0.2966104322449006 1102 82-83 0.037727103123536665 1102 84-85 0.08058548285293554 1102 86-87 0.14391624365400446 1102 88-89 0.048122836409259406 1102 90-91 -0.08334682779075919 1102 92-93 -0.07771392077644634 1102 94-95 -0.09200302674177507 1102 96-97 -0.27561185073770744 1102 98-99 -0.4807025242108516 1102 100-101 -0.5586891417961724 1103 1 0.3633023757409397 1103 2 0.4479998784143291 1103 3 0.3963254847733886 1103 4 0.4362473485502605 1103 5 0.43123766057070156 1103 6 0.3416102757658308 1103 7 0.35276681374298846 1103 8 0.3422911387797498 1103 9 0.42697335554387195 1103 10-11 0.491653645255397 1103 12-13 0.3983288284275446 1103 14-15 0.6083457629883426 1103 16-17 0.6077704753580591 1103 18-19 0.6592930433142996 1103 20-21 0.6304554022509663 1103 22-23 0.6612250893104772 1103 24-25 0.5795463050491279 1103 26-27 0.5779280181581541 1103 28-29 0.6405615982036252 1103 30-31 0.6805058838611799 1103 32-33 0.6203063451777666 1103 34-35 0.5548392714740586 1103 36-37 0.6594657478913604 1103 38-39 0.8170141001195006 1103 40-41 0.7297356302408744 1103 42-43 0.8011819281356907 1103 44-45 0.8397684801159713 1103 46-47 0.7449140422708069 1103 48-49 0.8164591571924049 1103 50-51 0.74791102784652 1103 52-53 0.8250799177993287 1103 54-55 0.9047481312320471 1103 56-57 0.7340811126389752 1103 58-59 0.7486265300508137 1103 60-61 0.7340578071208128 1103 62-63 0.6445725556394635 1103 64-65 0.8151228999500333 1103 66-67 0.7425674272854366 1103 68-69 0.7496705237159631 1103 70-71 0.7175236643289935 1103 72-73 0.708033727202924 1103 74-75 0.7652634722511671 1103 76-77 0.7813317940424263 1103 78-79 0.7888358034305973 1103 80-81 0.8353092233726507 1103 82-83 0.8116087992536762 1103 84-85 0.8848943814120354 1103 86-87 0.8394625435043501 1103 88-89 0.8716033361742141 1103 90-91 0.8858765267758244 1103 92-93 0.8718338842341424 1103 94-95 0.9251293842316635 1103 96-97 0.6543799765440745 1103 98-99 0.28019576318418515 1103 100-101 0.3289132328205291 1104 1 0.34360674543188807 1104 2 0.35028621359214895 1104 3 0.43060663527723975 1104 4 0.3353452500745959 1104 5 0.36542759461482177 1104 6 0.3278143040371333 1104 7 0.36079154243409306 1104 8 0.39135519659491536 1104 9 0.5100986004654473 1104 10-11 0.37660885308559955 1104 12-13 0.4778494283518171 1104 14-15 0.6077387924269857 1104 16-17 0.6420385771857795 1104 18-19 0.5073586081479888 1104 20-21 0.6554474487721862 1104 22-23 0.6529264419818119 1104 24-25 0.7085830062112493 1104 26-27 0.7144540380468314 1104 28-29 0.5924324883884537 1104 30-31 0.6704652382706868 1104 32-33 0.7207879863118336 1104 34-35 0.5918956475543027 1104 36-37 0.7229722047077018 1104 38-39 0.830833269494633 1104 40-41 0.6928007311216362 1104 42-43 0.6634256044986984 1104 44-45 0.5064316602316055 1104 46-47 0.7154728114368254 1104 48-49 0.6481271963864899 1104 50-51 0.7264733992756405 1104 52-53 0.5792355068994155 1104 54-55 0.6426049918117087 1104 56-57 0.6086691472929431 1104 58-59 0.6210018820543866 1104 60-61 0.7974944638760135 1104 62-63 0.6956735532211127 1104 64-65 0.6669792087571054 1104 66-67 0.6301198394678451 1104 68-69 0.6823325199231434 1104 70-71 0.5259136938148643 1104 72-73 0.42777685055060743 1104 74-75 0.41891028725573776 1104 76-77 0.3770109009993199 1104 78-79 0.534945019606976 1104 80-81 0.5347696295468687 1104 82-83 0.36038226512290095 1104 84-85 0.41844853424641215 1104 86-87 0.403606003549303 1104 88-89 0.4751199336759946 1104 90-91 0.47417888572791966 1104 92-93 0.5019866614480861 1104 94-95 0.4710261348213365 1104 96-97 0.46890609419425644 1104 98-99 0.3155897793512885 1104 100-101 0.04565992446332601 1105 1 0.34593648731040716 1105 2 0.300405269963683 1105 3 0.35755693438067837 1105 4 0.39722195320918985 1105 5 0.41035437810851505 1105 6 0.40034303448987174 1105 7 0.4867399023258514 1105 8 0.4482799135988671 1105 9 0.5550682711314963 1105 10-11 0.529358138233512 1105 12-13 0.485987022258918 1105 14-15 0.6333138758099253 1105 16-17 0.46642904571924504 1105 18-19 0.4236054943811709 1105 20-21 0.6295816417462845 1105 22-23 0.6768757655503066 1105 24-25 0.6726038307991544 1105 26-27 0.6237352174163959 1105 28-29 0.628827096225784 1105 30-31 0.7520166787658198 1105 32-33 0.7005288371142768 1105 34-35 0.8931780332985255 1105 36-37 0.8709325540345887 1105 38-39 0.9919321139521458 1105 40-41 0.9556146079085721 1105 42-43 0.8230990927950472 1105 44-45 0.8905575366517269 1105 46-47 0.9163421956877116 1105 48-49 0.8564571698952577 1105 50-51 0.7293819203441174 1105 52-53 0.8073358739503647 1105 54-55 0.9404137142324842 1105 56-57 0.991525602938907 1105 58-59 0.9323243129546555 1105 60-61 0.8710815390657203 1105 62-63 0.8537727660735115 1105 64-65 0.7134260164099189 1105 66-67 0.81842802550252 1105 68-69 0.7785375387895499 1105 70-71 0.5897376129197767 1105 72-73 0.7978736806944795 1105 74-75 0.837101622544882 1105 76-77 0.8020563772129456 1105 78-79 0.7079510823115776 1105 80-81 0.6656711613897066 1105 82-83 0.6163629504796759 1105 84-85 0.6333326361099694 1105 86-87 0.6706722324110679 1105 88-89 0.6674074366306613 1105 90-91 0.7019120437179396 1105 92-93 0.7932807828618706 1105 94-95 0.8559558986770561 1105 96-97 0.7823295024477197 1105 98-99 0.7016697890275836 1105 100-101 0.3673003765929437 1106 1 0.1797108204561617 1106 2 0.242760579550108 1106 3 0.15060610837786115 1106 4 0.3460693957808658 1106 5 0.2793756283890687 1106 6 0.2884634440676166 1106 7 0.301498315958014 1106 8 0.36289585036855954 1106 9 0.43352830472768034 1106 10-11 0.41516645829693744 1106 12-13 0.3444949738437657 1106 14-15 0.3900777258741712 1106 16-17 0.41393163365931684 1106 18-19 0.5085470374377081 1106 20-21 0.48820561590621736 1106 22-23 0.5316701395291688 1106 24-25 0.5966442024742875 1106 26-27 0.6450830861643624 1106 28-29 0.5231171321386157 1106 30-31 0.45304892810052166 1106 32-33 0.6138357427665468 1106 34-35 0.6566083827896918 1106 36-37 0.7015591690621861 1106 38-39 0.8354265922848114 1106 40-41 0.8453554376115662 1106 42-43 0.7795445411847624 1106 44-45 0.8577845324918272 1106 46-47 0.7054108736403961 1106 48-49 0.8191828509078647 1106 50-51 0.8565771558357298 1106 52-53 0.6322804235214647 1106 54-55 0.7627312694458368 1106 56-57 0.8527253542838054 1106 58-59 0.8696411450461383 1106 60-61 1.0234868362326992 1106 62-63 0.9815469741695466 1106 64-65 0.8484466467631293 1106 66-67 0.8120327205303894 1106 68-69 0.7201958577222101 1106 70-71 0.7442395834031004 1106 72-73 0.7769764090399249 1106 74-75 0.8055661925192332 1106 76-77 0.8670769106431244 1106 78-79 0.8731109758634794 1106 80-81 0.7996438826863788 1106 82-83 0.7579398540623501 1106 84-85 0.7264064969744801 1106 86-87 0.8190556036869161 1106 88-89 0.8885751615638462 1106 90-91 0.7906335851666952 1106 92-93 0.7944164927860484 1106 94-95 0.6576459513518742 1106 96-97 0.6568077096832354 1106 98-99 0.5264489042799063 1106 100-101 0.4239573072456544 1107 1 0.04367534387291627 1107 2 0.10393272258050601 1107 3 0.10366374857333582 1107 4 0.23970042095564992 1107 5 0.24991430998713327 1107 6 0.07521280815341669 1107 7 0.1267410573019916 1107 8 0.055731286604519426 1107 9 0.2113023936800147 1107 10-11 0.22369564558398736 1107 12-13 0.2517142713111937 1107 14-15 0.22446981258164556 1107 16-17 0.28520801382580174 1107 18-19 0.33000050843800466 1107 20-21 0.34466679624933505 1107 22-23 0.2967500730504895 1107 24-25 0.15447570285444812 1107 26-27 0.21833109515106486 1107 28-29 0.1081805094856847 1107 30-31 0.27615456959058804 1107 32-33 0.28804521084457235 1107 34-35 0.31940532038728264 1107 36-37 0.3333636233370427 1107 38-39 0.5386514541942375 1107 40-41 0.35506255152352395 1107 42-43 0.32622502461924796 1107 44-45 0.40059111425140514 1107 46-47 0.4802198726086786 1107 48-49 0.5553407299759527 1107 50-51 0.3968145916206893 1107 52-53 0.43692836171899785 1107 54-55 0.4057394424921412 1107 56-57 0.44202710135387235 1107 58-59 0.3843722577912203 1107 60-61 0.3478190424182017 1107 62-63 0.5330836451158589 1107 64-65 0.7152903589092077 1107 66-67 0.3550954640042008 1107 68-69 0.5705474360466454 1107 70-71 0.33737504813977637 1107 72-73 0.29879432994655275 1107 74-75 0.5616817359657595 1107 76-77 0.48425275314213323 1107 78-79 0.516629839579462 1107 80-81 0.48684920373846197 1107 82-83 0.5514255274202426 1107 84-85 0.4042669951509623 1107 86-87 0.37689572140520156 1107 88-89 0.5049636514850988 1107 90-91 0.47804490595427396 1107 92-93 0.45789320229812347 1107 94-95 0.2874919114439827 1107 96-97 0.3331540054886979 1107 98-99 0.4577732548999798 1107 100-101 0.4670189622136043 1108 1 0.18031480202170158 1108 2 0.2275233772621732 1108 3 0.22444192460871548 1108 4 0.22480924467385677 1108 5 0.23030305634535608 1108 6 0.1696179790142125 1108 7 0.17804795654557637 1108 8 0.22230580874715855 1108 9 0.17656701411704745 1108 10-11 0.19913887109787964 1108 12-13 0.19730888303073613 1108 14-15 0.2545086685814937 1108 16-17 0.33555231339282443 1108 18-19 0.20875824495436035 1108 20-21 0.30952504898208844 1108 22-23 0.3698030799644414 1108 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13 13705.0 14 12708.0 15 13568.0 16 16729.0 17 21538.0 18 18597.0 19 13787.0 20 14284.0 21 15398.0 22 16721.0 23 18819.0 24 21428.0 25 24221.0 26 28064.0 27 33145.0 28 38805.0 29 46260.0 30 55100.0 31 67541.0 32 83625.0 33 107353.0 34 146214.0 35 207244.0 36 316486.0 37 488489.0 38 459784.0 39 73941.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.37498855210299 19.000951309928194 14.6523180856971 22.971742052271715 2 14.993363235495796 20.24445862081321 40.808215008930695 23.953963134760293 3 19.19893075440302 24.879977593620772 29.7155131225596 26.205578529416613 4 12.760665947228379 16.42984241006335 37.324019078844394 33.48547256386388 5 13.93651723414939 37.63658779618652 33.56396780262072 14.862927167043368 6 31.570699700954354 35.763595463067965 17.99007571844019 14.675629117537495 7 28.74991252228109 32.97683590003252 20.446362779362666 17.82688879832372 8 26.30376843192097 34.90980513561958 19.896964851626585 18.88946158083286 9 26.97305181292332 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28.72608549055029 22.460196861528757 34-35 22.586934562709114 26.80335698929121 28.41677867833856 22.192929769661117 36-37 22.70483609850316 26.412804767022593 28.675918352516728 22.20644078195752 38-39 22.391948672872115 26.571823120611608 28.752507835923087 22.28372037059319 40-41 22.43931972721735 26.655139767266412 28.710811430986354 22.19472907452989 42-43 22.297230356684352 26.655205950270755 28.39266238950364 22.65490130354125 44-45 22.35825726517957 26.8709463002184 28.178616694947095 22.592179739654938 46-47 22.509083860115673 26.973093277066145 27.796560344060556 22.72126251875762 48-49 22.674487864267594 26.782165043318084 27.895347850529845 22.647999241884477 50-51 22.635715343007202 27.10002483532405 27.75393912393446 22.510320697734286 52-53 22.64816598669903 27.20207735239142 27.57093622480263 22.578820436106923 54-55 22.308835872087574 26.955856350147034 27.79010102586396 22.945206751901434 56-57 22.474580188747385 27.36977441529791 27.565414610690457 22.590230785264247 58-59 22.5612483833134 26.862790250440955 27.798054281052142 22.7779070851935 60-61 22.548559994076502 26.860564955624298 28.07488191677115 22.51599313352805 62-63 22.53491824202606 26.81955454576977 27.959340619422125 22.68618659278205 64-65 22.35508687839997 26.766632071251 28.00455197766789 22.873729072681133 66-67 22.279627677344042 26.963330570768928 27.80525201070504 22.951789741181987 68-69 22.193386817411042 27.017464233589905 27.884563761715302 22.90458518728375 70-71 22.20864862520869 27.0166961438113 27.808686620267263 22.965968610712746 72-73 22.286149387234484 26.82267262347547 27.720942543387235 23.170235445902815 74-75 22.20098141740425 27.135445883813507 27.538055371398997 23.125517327383246 76-77 22.266981324485922 26.873806305432225 27.56673748669544 23.29247488338641 78-79 22.29312364234891 26.88657389491716 27.717288670804763 23.10301379192917 80-81 22.14781279203775 27.22384167302339 27.598826400377767 23.029519134561088 82-83 22.308015586883997 27.04224881135354 27.515519385970233 23.13421621579223 84-85 22.225041125831112 26.78464638390976 27.538422126388674 23.451890363870454 86-87 22.24822369162519 26.57675621382827 27.763851613694264 23.411168480852282 88-89 22.281531701244557 26.434202227752667 27.787555262861808 23.496710808140968 90-91 22.052005306874676 26.672001073764523 27.97606744067193 23.29992617868887 92-93 22.15242383020758 26.409974321680217 27.90049795622379 23.537103891888407 94-95 21.958023417783988 26.48518909614853 27.8791844535582 23.677603032509282 96-97 22.015551481656924 26.299415580473422 27.88462928325494 23.800403654614712 98-99 22.053814770155764 26.24888505310451 27.73110371483788 23.966196461901852 100-101 22.15687415932404 25.883076165964404 28.182926134463564 23.777123540247995 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1850.0 1 1064.5 2 331.5 3 397.5 4 353.5 5 363.5 6 634.0 7 1289.5 8 2062.0 9 2389.0 10 2266.0 11 2184.0 12 2397.5 13 2694.0 14 2906.0 15 3146.0 16 3381.5 17 3523.5 18 3625.5 19 3719.0 20 4008.0 21 4478.5 22 5143.5 23 6328.0 24 7727.0 25 9278.5 26 11816.0 27 14962.0 28 18105.5 29 22087.5 30 26140.0 31 30433.5 32 35127.5 33 39741.5 34 45330.5 35 49801.0 36 54906.0 37 60795.5 38 64445.5 39 66115.5 40 68514.5 41 74860.0 42 80477.5 43 83454.5 44 87602.0 45 91973.5 46 93998.0 47 97298.5 48 108941.0 49 108583.0 50 96150.5 51 90000.0 52 85866.5 53 83663.0 54 80355.5 55 73742.0 56 65343.5 57 58161.5 58 52334.0 59 45466.0 60 38299.5 61 31645.5 62 25937.5 63 20850.5 64 16208.5 65 12526.0 66 9751.0 67 8239.5 68 6531.0 69 4479.5 70 3186.0 71 2409.5 72 1934.5 73 1586.5 74 1191.0 75 932.0 76 656.5 77 398.0 78 266.0 79 172.5 80 106.5 81 72.5 82 39.5 83 33.0 84 28.5 85 19.5 86 16.5 87 15.5 88 12.5 89 11.0 90 8.5 91 7.0 92 5.0 93 3.0 94 4.0 95 3.5 96 3.0 97 3.0 98 2.5 99 1.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2789198315291307 2 0.10963441755532939 3 0.12037159691065433 4 0.12283991400383247 5 0.1297923404829509 6 0.058046590307906105 7 0.06648000704293144 8 0.07125208675640919 9 0.06841352209925432 10-11 0.06386770478598457 12-13 0.06820782900815614 14-15 0.06384713547687475 16-17 0.06504015540524419 18-19 0.06933914100919612 20-21 0.07392609694068551 22-23 0.0827503305487974 24-25 0.09112203935649328 26-27 0.0918419651753369 28-29 0.08511580109642645 30-31 0.08094023134713342 32-33 0.07359698799492843 34-35 0.24006440662068468 36-37 0.36337741473404295 38-39 0.33525916918092186 40-41 0.3199761725123272 42-43 0.34260241253312684 44-45 0.3552731069447747 46-47 0.36162902345970843 48-49 0.37072065808624793 50-51 0.36417961778932584 52-53 0.3651875139357069 54-55 0.35194087886898423 56-57 0.32873869819310964 58-59 0.31429904319801744 60-61 0.26997218206635987 62-63 0.3410391450407807 64-65 0.37030927190405155 66-67 0.37613038638213003 68-69 0.3867030112645764 70-71 0.3882868480660324 72-73 0.37187253939639775 74-75 0.37454654958067407 76-77 0.35807053298370994 78-79 0.3370692683825859 80-81 0.336267065327303 82-83 0.3502130569037595 84-85 0.3565278348004736 86-87 0.3499662251944417 88-89 0.36442644949864367 90-91 0.3874640757016397 92-93 0.38291825838836996 94-95 0.38312395147946815 96-97 0.38789603119294586 98-99 0.37707657460118166 100-101 0.3557667703634103 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2430806.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.766017975413206 #Duplication Level Percentage of deduplicated Percentage of total 1 72.38550714436737 36.747239568503545 2 14.555423664951702 14.778417988293857 3 5.423115353224609 8.259299145136195 4 2.606558573299724 5.292983975444048 5 1.3989471538052811 3.550948817836602 6 0.8651712443152316 2.6352779364430594 7 0.5586747615294148 1.985318509034837 8 0.36405591061006026 1.4785335121668595 9 0.2798063579881237 1.278418913933399 >10 1.3991771611097643 12.683498261056226 >50 0.1014343470719046 3.5436182682357162 >100 0.058550001215767075 5.400811768962696 >500 0.0022771168706302875 0.7987557920968039 >1k 0.0011385584353142718 1.044075837407577 >5k 1.6265120504489598E-4 0.522801705448699 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7306 0.3005587447126591 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5357 0.22037957780258896 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3800 0.156326749234616 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2944 0.12111209203860776 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 4.1138618219635794E-5 0.0 8 0.0 0.0 0.0 4.1138618219635794E-5 0.0 9 0.0 0.0 0.0 4.1138618219635794E-5 0.0 10-11 0.0 0.0 0.0 4.1138618219635794E-5 0.0 12-13 0.0 0.0 0.0 4.1138618219635794E-5 0.0 14-15 0.0 0.0 0.0 4.1138618219635794E-5 0.0 16-17 0.0 0.0 0.0 4.1138618219635794E-5 0.0 18-19 0.0 0.0 0.0 4.1138618219635794E-5 0.0 20-21 0.0 0.0 0.0 1.0284654554908949E-4 0.0 22-23 0.0 0.0 0.0 1.4398516376872526E-4 0.0 24-25 0.0 0.0 0.0 2.4683170931781475E-4 0.0 26-27 0.0 0.0 0.0 5.96509964184719E-4 0.0 28-29 0.0 0.0 0.0 0.002797426038935234 0.0 30-31 0.0 0.0 0.0 0.0091944811720886 0.0 32-33 0.0 0.0 0.0 0.022112007293054237 0.0 34-35 0.0 0.0 0.0 0.043956613567680845 0.0 36-37 0.0 0.0 0.0 0.07614758232454585 0.0 38-39 0.0 0.0 0.0 0.12335414673157793 0.0 40-41 0.0 0.0 0.0 0.1769166276535437 0.0 42-43 0.0 0.0 0.0 0.23564200516207381 0.0 44-45 0.0 0.0 0.0 0.30794312668308377 0.0 46-47 0.0 0.0 0.0 0.3969465272012658 0.0 48-49 2.0569309109817897E-5 0.0 0.0 0.4950827009642069 0.0 50-51 4.1138618219635794E-5 0.0 0.0 0.6039149154642534 0.0 52-53 4.1138618219635794E-5 0.0 0.0 0.713528763710473 0.0 54-55 4.1138618219635794E-5 0.0 0.0 0.8385284551708363 0.0 56-57 4.1138618219635794E-5 0.0 0.0 0.9870388669437216 0.0 58-59 4.1138618219635794E-5 0.0 0.0 1.1513670774220568 0.0 60-61 4.1138618219635794E-5 0.0 0.0 1.3343105126447772 0.0 62-63 4.1138618219635794E-5 0.0 0.0 1.5313850632259425 0.0 64-65 4.1138618219635794E-5 0.0 0.0 1.73948476348997 0.0 66-67 4.1138618219635794E-5 0.0 0.0 1.9703958275567857 0.0 68-69 4.1138618219635794E-5 0.0 0.0 2.2367889498380373 0.0 70-71 4.1138618219635794E-5 0.0 0.0 2.53691573905939 0.0 72-73 4.1138618219635794E-5 0.0 0.0 2.847368321453872 0.0 74-75 4.1138618219635794E-5 0.0 0.0 3.1740089501177797 0.0 76-77 4.1138618219635794E-5 0.0 0.0 3.532285176192588 0.0 78-79 4.1138618219635794E-5 0.0 0.0 3.928203237938363 0.0 80-81 4.1138618219635794E-5 0.0 0.0 4.370031997617252 0.0 82-83 4.1138618219635794E-5 0.0 0.0 4.82547763992684 0.0 84-85 4.1138618219635794E-5 0.0 0.0 5.300279002108766 0.0 86-87 4.1138618219635794E-5 0.0 0.0 5.808279229193938 0.0 88-89 4.1138618219635794E-5 0.0 0.0 6.361634782866259 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 15650 0.0 27.25411 1 GTACATG 12895 0.0 25.010273 1 TACATGG 12810 0.0 24.765081 2 ACATGGG 13085 0.0 22.903727 3 ATCAACG 18460 0.0 22.888052 3 TCAACGC 18495 0.0 22.844738 4 TATCAAC 18650 0.0 22.807432 2 TAGCGCG 125 2.2827672E-4 22.761665 4 GAGTACT 7270 0.0 22.750685 12-13 CAACGCA 18635 0.0 22.647665 5 AACGCAG 19140 0.0 22.209818 6 GGTATCA 11455 0.0 22.18343 1 AGTACTT 7585 0.0 21.118572 12-13 GTACTTT 7880 0.0 21.019814 14-15 CATGGGG 4940 0.0 20.542326 4 ACGCAGA 21010 0.0 19.962353 7 CGCAGAG 21035 0.0 19.93863 8 ATACCGT 265 1.9070285E-8 19.671675 6 CATGGGA 8405 0.0 19.46454 4 GCAGAGT 21465 0.0 19.16348 9 >>END_MODULE