FastQCFastQC Report
Fri 27 May 2016
SRR939280_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939280_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2430806
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73770.30347958660625324No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59040.2428824019687297No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42380.1743454640148165No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA28830.11860263632720999No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA24920.10251743660333239No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA154450.025.514031
GTACATG128450.023.9350911
TAGGTCG1601.875289E-623.729095
TACATGG128400.023.5073242
GGTATCA114600.022.1765161
ACATGGG131250.022.1652323
TCAACGC180150.021.7335554
ATCAACG180450.021.6711243
TATCAAC183750.021.462722
CAACGCA182600.021.415965
CATGGGG51750.020.9091114
AACGCAG187500.020.8562896
GAGTACT78350.020.65633412-13
AGTACTT82000.020.08415212-13
GTACCGT1708.012547E-519.5416056
GTACTTT84750.019.04044514-15
ACGCAGA207350.018.6994617
CGCAGAG208900.018.5607158
GGTACGT1300.007604012318.2531472
CATGGGA80750.018.1016964
Produced by FastQC (version 0.11.5)