##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939280_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2430806 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.250639911206406 33.0 31.0 34.0 31.0 34.0 2 32.45014369719344 34.0 31.0 34.0 31.0 34.0 3 32.54180053858679 34.0 31.0 34.0 31.0 34.0 4 36.08771164790608 37.0 35.0 37.0 35.0 37.0 5 36.02261636675243 37.0 35.0 37.0 35.0 37.0 6 36.056929676823245 37.0 35.0 37.0 35.0 37.0 7 36.000218446062746 37.0 35.0 37.0 35.0 37.0 8 35.97937926761741 37.0 35.0 37.0 35.0 37.0 9 37.67643448304801 39.0 37.0 39.0 35.0 39.0 10-11 37.59236648255764 39.0 37.0 39.0 35.0 39.0 12-13 37.513396379637044 39.0 37.0 39.0 35.0 39.0 14-15 38.79953994683245 40.0 38.0 41.0 35.0 41.0 16-17 38.74916180065378 40.0 38.0 41.0 34.0 41.0 18-19 38.86520252953136 40.0 38.0 41.0 35.0 41.0 20-21 38.87082700141434 40.0 38.0 41.0 34.5 41.0 22-23 38.81549843961221 40.0 38.0 41.0 34.0 41.0 24-25 38.75206927249645 40.0 38.0 41.0 34.0 41.0 26-27 38.58558293010631 40.0 38.0 41.0 34.0 41.0 28-29 38.436777554440795 40.0 38.0 41.0 34.0 41.0 30-31 38.259446866594864 40.0 38.0 41.0 34.0 41.0 32-33 38.0760169672117 40.0 37.5 41.0 33.0 41.0 34-35 38.04497150327916 40.0 37.5 41.0 33.0 41.0 36-37 37.96790344437195 40.0 37.0 41.0 33.0 41.0 38-39 37.871594648030324 40.0 37.0 41.0 33.0 41.0 40-41 37.69946511568591 40.0 37.0 41.0 33.0 41.0 42-43 37.53487896607133 40.0 37.0 41.0 32.5 41.0 44-45 37.316016169122506 40.0 37.0 41.0 32.0 41.0 46-47 37.02923063378978 40.0 36.5 41.0 31.0 41.0 48-49 36.72864329773746 40.0 36.0 41.0 30.5 41.0 50-51 36.50026904656316 39.0 36.0 41.0 30.0 41.0 52-53 36.276949291716406 39.0 35.0 41.0 30.0 41.0 54-55 36.003888216501025 39.0 35.0 40.0 29.0 41.0 56-57 35.77620797381609 38.5 35.0 40.0 28.5 41.0 58-59 35.492641329665965 38.0 35.0 40.0 28.0 41.0 60-61 35.16755800339476 38.0 34.0 40.0 28.0 41.0 62-63 34.78963911558553 37.5 34.0 40.0 27.0 41.0 64-65 34.37820130442331 37.0 34.0 40.0 26.0 41.0 66-67 34.0102686104938 36.0 33.0 39.0 26.0 40.5 68-69 33.633018842309916 36.0 33.0 39.0 26.0 40.0 70-71 33.24801197627454 35.5 33.0 38.5 25.5 40.0 72-73 32.760256885987616 35.0 32.0 37.5 24.5 40.0 74-75 32.266296446528436 35.0 32.0 37.0 23.0 39.0 76-77 30.256583413073688 33.5 29.5 35.0 20.0 37.5 78-79 31.247929287651914 34.0 31.0 36.0 20.5 38.0 80-81 31.302371929310688 35.0 31.0 36.0 21.0 37.5 82-83 30.999646824962582 34.0 31.0 35.0 20.0 37.0 84-85 30.633069442810324 34.0 31.0 35.0 19.0 36.5 86-87 30.10233272420753 34.0 30.5 35.0 15.5 36.0 88-89 29.611153872419273 34.0 30.0 35.0 7.5 36.0 90-91 29.201867199603754 34.0 29.0 35.0 4.5 35.0 92-93 28.866354410841506 34.0 29.0 35.0 2.0 35.0 94-95 28.4792546176042 34.0 29.0 35.0 2.0 35.0 96-97 27.942469905043843 33.5 29.0 35.0 2.0 35.0 98-99 27.199851613004082 33.0 27.0 35.0 2.0 35.0 100-101 23.86726316291798 30.0 13.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21903687382860682 1101 2 0.29550186402180145 1101 3 0.31940581078227837 1101 4 0.22614304785596318 1101 5 0.2671794580587914 1101 6 0.29256313230921904 1101 7 0.2555403935356395 1101 8 0.3091386503754592 1101 9 0.3345041157828206 1101 10-11 0.3359355009199234 1101 12-13 0.3422791560756835 1101 14-15 0.4043617417400682 1101 16-17 0.393491963204994 1101 18-19 0.38466863689362185 1101 20-21 0.3757032082698757 1101 22-23 0.379397291823679 1101 24-25 0.4789182218998249 1101 26-27 0.5717418134589849 1101 28-29 0.5500075096264538 1101 30-31 0.5447331582571806 1101 32-33 0.45916244714814525 1101 34-35 0.4483587903461981 1101 36-37 0.572592192839906 1101 38-39 0.526326001183854 1101 40-41 0.7006951732412929 1101 42-43 0.5650763423161109 1101 44-45 0.712360266067293 1101 46-47 0.7704160470480019 1101 48-49 0.715307395296314 1101 50-51 0.7500380933031536 1101 52-53 0.6396030068961238 1101 54-55 0.6011922344008127 1101 56-57 0.5778567480836614 1101 58-59 0.6858143277962085 1101 60-61 0.5917003733310437 1101 62-63 0.6796886052116662 1101 64-65 0.7427690722402147 1101 66-67 0.6616464716858488 1101 68-69 0.6632602634241493 1101 70-71 0.5463158705536628 1101 72-73 0.47672257873411183 1101 74-75 0.4548306120641996 1101 76-77 0.552957529481457 1101 78-79 0.7516792561137997 1101 80-81 0.8354023924402334 1101 82-83 0.887302313798827 1101 84-85 0.7700827282584264 1101 86-87 0.9383185320631746 1101 88-89 0.9100053426708108 1101 90-91 0.8913363054589851 1101 92-93 0.9223034988183265 1101 94-95 1.1148076714148338 1101 96-97 1.0557690892568417 1101 98-99 1.0205337901476064 1101 100-101 0.6775806512944982 1102 1 0.09987799532393637 1102 2 0.15297849953581988 1102 3 0.14323758648321672 1102 4 0.04249818804288452 1102 5 0.10783366366626268 1102 6 0.11639490801015029 1102 7 0.21301702904965936 1102 8 0.1525965943006895 1102 9 0.24338262045571213 1102 10-11 0.20719718316291846 1102 12-13 0.21681186635606053 1102 14-15 0.31767949874941337 1102 16-17 0.3572770099339664 1102 18-19 0.20686489857585855 1102 20-21 0.16495554471848095 1102 22-23 0.1765935535059171 1102 24-25 0.11980607236711904 1102 26-27 0.19861097233749092 1102 28-29 0.2593533040189726 1102 30-31 0.2601537190048404 1102 32-33 0.10659235369020337 1102 34-35 0.30957374361722145 1102 36-37 0.29221836106421506 1102 38-39 0.32095216940815163 1102 40-41 0.29625592090484787 1102 42-43 0.29241279091424843 1102 44-45 0.4553509202728989 1102 46-47 0.39541604704800903 1102 48-49 0.32068122707202207 1102 50-51 0.3427951026489424 1102 52-53 0.40081796016714577 1102 54-55 0.29698662692416633 1102 56-57 0.27201562658832756 1102 58-59 0.2379171315345303 1102 60-61 0.1470742051067404 1102 62-63 0.27267925941727356 1102 64-65 0.2862270161654479 1102 66-67 0.2422539483213626 1102 68-69 0.3265780204334945 1102 70-71 0.21734390793684355 1102 72-73 0.3781244478929935 1102 74-75 0.34478388309224073 1102 76-77 0.29010706219174054 1102 78-79 0.22644561125398965 1102 80-81 0.28750519617854664 1102 82-83 0.401554650247423 1102 84-85 0.32615749461356813 1102 86-87 0.4285054479510251 1102 88-89 0.6607062772502488 1102 90-91 0.6509157447113232 1102 92-93 0.7473034988183223 1102 94-95 0.7120039330970762 1102 96-97 0.5688532014063767 1102 98-99 0.49716930416630234 1102 100-101 0.17010401578048118 1103 1 0.15481354082591992 1103 2 0.1936656979107667 1103 3 0.16172496513236467 1103 4 0.1421986654676317 1103 5 0.1185116758196827 1103 6 0.09421708346267366 1103 7 0.17737082364919843 1103 8 0.10506566088570679 1103 9 0.11027319228722376 1103 10-11 0.17516497963419653 1103 12-13 0.20074093837517637 1103 14-15 0.21639051660864084 1103 16-17 0.16276793368832898 1103 18-19 0.1553568272079815 1103 20-21 0.21068384388740924 1103 22-23 0.20915687589917553 1103 24-25 0.23368211875537526 1103 26-27 0.33568546267470367 1103 28-29 0.18921930594247272 1103 30-31 0.18540989814047037 1103 32-33 0.20638609498377747 1103 34-35 0.3282586475931737 1103 36-37 0.4525205144352924 1103 38-39 0.4235911595275681 1103 40-41 0.32698286154805345 1103 42-43 0.30036307749848135 1103 44-45 0.40582586517713537 1103 46-47 0.32865388824281894 1103 48-49 0.14484842924220942 1103 50-51 0.21525304226140207 1103 52-53 0.16296382701438006 1103 54-55 0.2524926454087435 1103 56-57 0.2403062346471927 1103 58-59 0.19032085950161104 1103 60-61 0.016493252068606523 1103 62-63 0.10557434241672325 1103 64-65 0.2489154307443684 1103 66-67 0.19904021556708784 1103 68-69 0.05822304413221957 1103 70-71 0.18887050269508876 1103 72-73 0.22148125712578803 1103 74-75 0.032639202259524325 1103 76-77 0.1613555904555426 1103 78-79 0.19416984347131816 1103 80-81 0.13614623918127933 1103 82-83 0.09499749944244584 1103 84-85 0.06203432592780089 1103 86-87 0.12554925285810725 1103 88-89 0.4390684127664244 1103 90-91 0.5850647685135577 1103 92-93 0.5900721526702846 1103 94-95 0.5573049435814426 1103 96-97 0.18560457826141175 1103 98-99 -0.034348384701122114 1103 100-101 0.04686395350393724 1104 1 0.13558497027317884 1104 2 0.15304309793512516 1104 3 0.143361749640313 1104 4 0.07801388461496828 1104 5 0.11654857499028282 1104 6 0.10540311284580639 1104 7 0.11231903076109973 1104 8 0.12879250362802708 1104 9 0.18909983047601742 1104 10-11 0.1979726156366155 1104 12-13 0.22881961816397478 1104 14-15 0.3003927154141195 1104 16-17 0.1723976495420203 1104 18-19 0.19787355643924798 1104 20-21 0.22012383613276398 1104 22-23 0.21814710306212248 1104 24-25 0.15819304194714334 1104 26-27 0.1819718508757262 1104 28-29 0.26070357835243385 1104 30-31 0.2992961542805972 1104 32-33 0.10222483074519317 1104 34-35 0.25021368724043924 1104 36-37 0.3106284853951564 1104 38-39 0.30958746530237846 1104 40-41 0.4757975239518757 1104 42-43 0.4872742818788609 1104 44-45 0.5540404956013418 1104 46-47 0.39370754522750673 1104 48-49 0.20813865085430194 1104 50-51 0.23088173161922754 1104 52-53 0.10085067883692034 1104 54-55 0.07741876504729106 1104 56-57 0.2629416488370282 1104 58-59 0.18954425615071102 1104 60-61 0.27595136747523696 1104 62-63 0.3338744975924044 1104 64-65 0.2720527011601632 1104 66-67 0.2505376443565339 1104 68-69 0.33865959898346887 1104 70-71 0.2244543460314361 1104 72-73 0.45975836792520397 1104 74-75 0.40907103556123303 1104 76-77 0.5083913454850837 1104 78-79 0.5182143489844862 1104 80-81 0.4702293190665152 1104 82-83 0.49918044933801653 1104 84-85 0.45720457594043395 1104 86-87 0.46529255847301343 1104 88-89 0.6707974700554971 1104 90-91 0.7750759655203971 1104 92-93 0.7880327895782351 1104 94-95 0.9174285207579445 1104 96-97 0.7925884989784819 1104 98-99 0.8125693108778229 1104 100-101 0.6537899836658454 1105 1 0.1530270053940157 1105 2 0.1513665454787798 1105 3 0.21374236637761612 1105 4 0.1137618250425163 1105 5 0.2101094119265312 1105 6 0.2099452399072561 1105 7 0.14243076666782173 1105 8 0.25369516753410437 1105 9 0.25770645984467677 1105 10-11 0.2671934999898724 1105 12-13 0.22710808400483984 1105 14-15 0.3375498057902462 1105 16-17 0.364328022909433 1105 18-19 0.25014886915986523 1105 20-21 0.2222759025829788 1105 22-23 0.2842060960475976 1105 24-25 0.2921692085375227 1105 26-27 0.3047119735844177 1105 28-29 0.2723361803507771 1105 30-31 0.3017074213139139 1105 32-33 0.2889422386682057 1105 34-35 0.3313738069924881 1105 36-37 0.3221952743594372 1105 38-39 0.2817599160058464 1105 40-41 0.43344742944199055 1105 42-43 0.34110896823330705 1105 44-45 0.4963587310689235 1105 46-47 0.515411458513988 1105 48-49 0.47908122789507246 1105 50-51 0.5088541486453977 1105 52-53 0.5321385114496735 1105 54-55 0.5679258772029812 1105 56-57 0.5446958448626305 1105 58-59 0.6442809961089893 1105 60-61 0.5337136492151089 1105 62-63 0.49653654983649176 1105 64-65 0.48904351184234685 1105 66-67 0.4987426086751938 1105 68-69 0.6158239782025205 1105 70-71 0.5544291394417584 1105 72-73 0.508905486342023 1105 74-75 0.49867765544333764 1105 76-77 0.7779312945896706 1105 78-79 0.7475189166857454 1105 80-81 0.7921478460878042 1105 82-83 0.7851754356892222 1105 84-85 0.8566304016864947 1105 86-87 0.7278303367356607 1105 88-89 0.7271007183338867 1105 90-91 0.8627562201081389 1105 92-93 0.9124393852695967 1105 94-95 0.920176461976034 1105 96-97 1.1603536108764487 1105 98-99 1.3232754906624287 1105 100-101 1.3103829081124836 1106 1 0.10882991107060747 1106 2 0.04493555002408556 1106 3 0.11037261848963453 1106 4 0.13879153385340715 1106 5 0.08957326410028088 1106 6 0.09949079820009388 1106 7 0.07968013272464702 1106 8 0.13610148904454178 1106 9 0.16318122403188084 1106 10-11 0.17809281843089053 1106 12-13 0.1979313045596669 1106 14-15 0.24293724422273044 1106 16-17 0.19372409547401048 1106 18-19 0.22643227743751027 1106 20-21 0.16680181305611796 1106 22-23 0.2377271293915939 1106 24-25 0.1656217420011501 1106 26-27 0.20769287005408898 1106 28-29 0.2074738595229917 1106 30-31 0.1878033747391683 1106 32-33 0.2657746874363056 1106 34-35 0.25307928373314326 1106 36-37 0.30872012171990804 1106 38-39 0.39977549476845553 1106 40-41 0.37351250470842956 1106 42-43 0.4210018663784112 1106 44-45 0.38757041534992 1106 46-47 0.5449272785389354 1106 48-49 0.4382542045364275 1106 50-51 0.4929588658135273 1106 52-53 0.4729483737296434 1106 54-55 0.38406335359699995 1106 56-57 0.5350645457917835 1106 58-59 0.5906085347751002 1106 60-61 0.5799295933585498 1106 62-63 0.5420234807153577 1106 64-65 0.5053130886326684 1106 66-67 0.47600902558449576 1106 68-69 0.5701406356424883 1106 70-71 0.440769300907931 1106 72-73 0.4564919792237845 1106 74-75 0.5471224493174915 1106 76-77 0.6300216108951453 1106 78-79 0.6990848603635804 1106 80-81 0.5853425946837056 1106 82-83 0.5474688399626153 1106 84-85 0.5522909293240978 1106 86-87 0.5208011171010298 1106 88-89 0.8394543764278026 1106 90-91 0.8405409852132628 1106 92-93 0.8100283202317691 1106 94-95 0.9749047192409392 1106 96-97 0.7159789763570643 1106 98-99 0.8276190680004554 1106 100-101 0.7275963822388789 1107 1 0.040980437644392964 1107 2 0.08867244146539832 1107 3 5.34558699456511E-5 1107 4 0.05806920861620313 1107 5 0.01990816288646613 1107 6 0.13750269302938278 1107 7 0.14858545684268876 1107 8 0.097782272725226 1107 9 0.023011420744850852 1107 10-11 0.06516124172832605 1107 12-13 0.07687067532707914 1107 14-15 0.1475040343078291 1107 16-17 0.08994122917319203 1107 18-19 0.19081863316159087 1107 20-21 0.145849798567383 1107 22-23 0.06106054073450906 1107 24-25 0.12611723350055826 1107 26-27 0.11164882692966671 1107 28-29 0.14084242416291204 1107 30-31 0.16830378991344475 1107 32-33 0.12430094482839849 1107 34-35 0.21077958287269638 1107 36-37 0.12264149494988885 1107 38-39 0.10412541820113574 1107 40-41 0.18440347760226672 1107 42-43 0.09548219099988131 1107 44-45 0.058060277459816234 1107 46-47 -0.038247178292103 1107 48-49 0.05948228225502561 1107 50-51 0.015802884918251436 1107 52-53 0.04394925306096553 1107 54-55 0.08686859649435519 1107 56-57 0.06432322068464913 1107 58-59 0.0366930249633981 1107 60-61 0.023149226811462142 1107 62-63 0.0883221707166868 1107 64-65 0.09903888638501002 1107 66-67 0.050118224130329736 1107 68-69 0.11484058265040176 1107 70-71 0.08229266124165235 1107 72-73 0.11907466258305277 1107 74-75 0.08346512039842224 1107 76-77 0.24022521047519874 1107 78-79 0.45368021465681707 1107 80-81 0.35037363614979355 1107 82-83 0.44042199810818516 1107 84-85 0.4088747479949859 1107 86-87 0.3838406826565688 1107 88-89 0.518472047050647 1107 90-91 0.5425901700765721 1107 92-93 0.4415625529249354 1107 94-95 0.6667449889872046 1107 96-97 0.6986549519259135 1107 98-99 0.7978804527286805 1107 100-101 0.8579260017716095 1108 1 -0.04344413533303282 1108 2 0.0037519445889984127 1108 3 0.035431776437640394 1108 4 -0.008114889930816105 1108 5 0.01328307514210536 1108 6 0.005776919274211423 1108 7 0.0038761055194314054 1108 8 -0.014007153224746105 1108 9 -0.08091414304504241 1108 10-11 0.04187637227330043 1108 12-13 0.008753612438958669 1108 14-15 0.1344785258046457 1108 16-17 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sequence quality scores pass #Quality Count 2 1.0 3 8.0 4 17.0 5 52.0 6 99.0 7 304.0 8 644.0 9 1141.0 10 1961.0 11 3055.0 12 4455.0 13 6405.0 14 7822.0 15 10390.0 16 13773.0 17 19223.0 18 18426.0 19 12337.0 20 11352.0 21 12323.0 22 13364.0 23 14785.0 24 17063.0 25 20266.0 26 24889.0 27 30214.0 28 37423.0 29 45530.0 30 56080.0 31 68669.0 32 85752.0 33 110090.0 34 148847.0 35 212643.0 36 333804.0 37 550583.0 38 492003.0 39 45011.0 40 2.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.53585919336821 19.65659655705543 15.14295001575775 22.664594233818605 2 15.468696391238131 20.907468551583303 39.914867743456284 23.70896731372228 3 19.232756542480146 24.95464467341285 30.042792390672062 25.769806393434934 4 13.12289010311806 17.107576663871985 36.904631632470874 32.864901600539085 5 13.906292809874584 37.85472802025336 33.53846419664917 14.700514973222873 6 31.63148354907796 36.23662274982043 17.559525523632903 14.572368177468709 7 28.606355258297043 33.196725695098664 20.342223937245507 17.85469510935879 8 26.27404243695301 35.36966750945982 19.594488412485404 18.76180164110176 9 26.709576988044294 17.221777468049694 20.144881985645913 35.923763558260106 10-11 25.107783179735442 26.922963000749544 27.021716253785783 20.947537565729228 12-13 25.93090522238303 24.190823948928873 27.722533184466386 22.15573764422171 14-15 22.81301019374429 25.49548895394868 26.285839937418633 25.405660914888394 16-17 22.055079899753004 28.93478103468987 26.80698010034573 22.203158965211394 18-19 21.982605769444376 27.42067857327981 29.20329717797307 21.39341847930275 20-21 23.13290324279272 26.463629759018204 28.781934880858444 21.62153211733063 22-23 22.569016202856172 26.476094760338754 28.817170107363566 22.13771892944151 24-25 22.34311726837761 26.64916080252443 28.994742275654005 22.012979653443953 26-27 22.37663101140088 26.738980992497464 28.90256901394081 21.981818982160846 28-29 21.993795473600112 26.97004614930192 29.186841730685213 21.849316646412753 30-31 22.260702828609112 26.690262406790175 29.01901673765821 22.030018026942503 32-33 22.103183059446128 26.786382788260354 28.750011313120012 22.36042283917351 34-35 22.562546320799214 26.826106551568586 28.57092607863824 22.04042104899396 36-37 22.685626560749334 26.412662020644994 28.845466219922205 22.056245198683467 38-39 22.41454499077413 26.584617789781813 28.889048881684644 22.11178833775942 40-41 22.449096310749038 26.649724314757435 28.828975988917044 22.07220338557648 42-43 22.3336946840307 26.6534057131808 28.528239865552855 22.484659737235646 44-45 22.443087093844717 26.82117700055229 28.29024219417701 22.445493711425982 46-47 22.53119648116281 27.038184501171013 27.86498947055767 22.565629547108507 48-49 22.75232988564287 26.835214328087066 27.913436942314608 22.49901884395546 50-51 22.661886633487 27.154655698562536 27.74559137997849 22.43786628797197 52-53 22.734290601553557 27.252647887161707 27.549257324525282 22.463804186759454 54-55 22.296452287841976 27.075463858489734 27.74460405314122 22.883479800527066 56-57 22.51884765793733 27.379293123350855 27.581468862591258 22.52039035612056 58-59 22.63312100233833 26.89250763947283 27.756771855803613 22.717599502385227 60-61 22.59647781810343 26.989352327620182 27.980068834268707 22.434101020007684 62-63 22.650202219623143 26.91277630613801 27.78827969538908 22.648741778849775 64-65 22.33660817371032 26.86712285289794 27.904001369093496 22.892267604298247 66-67 22.262800898138313 27.07875494794731 27.731069447746957 22.92737470616742 68-69 22.2069871704683 27.08565253056815 27.792683719502815 22.914676579460735 70-71 22.29355660088806 27.111404018133083 27.66076923883873 22.934270142140125 72-73 22.333847291803625 26.980495358329705 27.545554848885512 23.140102500981154 74-75 22.20911500136169 27.30557271950127 27.285620489664748 23.199691789472297 76-77 22.213071021199816 27.045310608484545 27.506448676124723 23.235169694190912 78-79 22.304309763921925 27.054771133525257 27.534385714038883 23.106533388513935 80-81 22.11823428161449 27.352718299939443 27.4576227258366 23.07142469260946 82-83 22.218762007334192 27.183103053061412 27.36917302326883 23.22896191633557 84-85 22.155223411493967 26.944190527750877 27.344469283027934 23.556116777727222 86-87 22.12868479908546 26.795736642725572 27.506042240708705 23.569536317480267 88-89 22.16582382134968 26.67443904978488 27.48855764977336 23.671179479092075 90-91 21.94282881030314 26.852682308174852 27.689052932777624 23.515435948744383 92-93 22.000871315933892 26.672572800955734 27.606727974178114 23.719827908932263 94-95 21.762246760950894 26.749049492226035 27.54736494808718 23.94133879873589 96-97 21.838620605675647 26.55804288783227 27.565507078722035 24.03782942777005 98-99 21.808507137138875 26.569993656425073 27.348521436922567 24.27297776951349 100-101 21.78458157490982 26.250910451479033 27.867209944711608 24.097298028899544 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 464.0 1 326.5 2 231.5 3 284.5 4 257.5 5 287.5 6 497.0 7 1103.5 8 1869.0 9 2213.0 10 2049.5 11 1846.5 12 1964.5 13 2165.5 14 2397.5 15 2681.0 16 2819.5 17 2884.0 18 3069.5 19 3387.5 20 3765.0 21 4213.0 22 4983.5 23 6286.5 24 7678.5 25 9223.5 26 11737.0 27 14837.5 28 18057.0 29 22174.0 30 26445.5 31 30803.5 32 35435.5 33 39915.0 34 45499.5 35 50193.0 36 55430.0 37 61053.5 38 64711.0 39 66559.5 40 69105.5 41 75708.0 42 81264.5 43 84285.5 44 88045.0 45 92600.5 46 94969.0 47 98055.5 48 109920.5 49 109973.0 50 97656.0 51 90530.5 52 85956.0 53 84148.5 54 80959.5 55 74033.5 56 65411.5 57 58258.0 58 52220.5 59 45193.0 60 37694.0 61 30939.0 62 25346.0 63 20352.5 64 15835.0 65 12196.5 66 9251.5 67 7738.5 68 6062.5 69 4031.0 70 2890.0 71 2127.5 72 1667.5 73 1350.5 74 1085.0 75 918.5 76 627.0 77 333.0 78 186.5 79 126.0 80 79.5 81 45.0 82 29.5 83 12.0 84 6.0 85 4.5 86 3.5 87 3.5 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27159715748603547 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0020775002200916076 16-17 4.1138618219635785E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 6.170792732945369E-5 26-27 0.0011930199283694378 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0027768567298254157 36-37 0.01986995260008409 38-39 0.005800545168968646 40-41 0.016763986924501588 42-43 0.004566386622379573 44-45 3.4967825486690424E-4 46-47 1.2341585465890738E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 4.1138618219635785E-4 60-61 0.0016455447287854314 62-63 0.001357574401247981 64-65 2.0569309109817894E-5 66-67 0.0 68-69 3.291089457570863E-4 70-71 2.0569309109817894E-5 72-73 0.0 74-75 0.0 76-77 0.22634467744443612 78-79 0.0 80-81 9.050496008319875E-4 82-83 0.0 84-85 0.0 86-87 0.10222946627579495 88-89 0.35192030955987436 90-91 0.35192030955987436 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 2.6740101842763264E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2430806.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.90745823399222 #Duplication Level Percentage of deduplicated Percentage of total 1 70.79283804918268 34.622977701561766 2 15.26303747762698 14.929527359217989 3 5.735436775750304 8.41516903691133 4 2.7460365146677863 5.372066646005293 5 1.5152195468551821 3.7052768351574215 6 0.9286926213892697 2.7251997355687467 7 0.6185245567367111 2.1175324747709476 8 0.4129848968389266 1.6158433274735513 9 0.313185320158251 1.378538816862523 >10 1.495006065616052 13.156947516883418 >50 0.11245939273566785 3.7993230165174223 >100 0.06244876991528371 5.610236516787724 >500 0.0027814370079040527 0.9240763883153221 >1k 0.0011800035791102764 1.079813578619414 >5k 1.6857193987289664E-4 0.5474710493469913 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7377 0.30347958660625324 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5904 0.2428824019687297 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4238 0.1743454640148165 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2883 0.11860263632720999 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2492 0.10251743660333239 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 4.1138618219635794E-5 0.0 22-23 0.0 0.0 0.0 1.0284654554908949E-4 0.0 24-25 0.0 0.0 0.0 2.0569309109817895E-4 0.0 26-27 0.0 0.0 0.0 5.553713459650832E-4 0.0 28-29 0.0 0.0 0.0 0.002797426038935234 0.0 30-31 0.0 0.0 0.0 0.009379604954076961 0.0 32-33 0.0 0.0 0.0 0.02250282416614078 0.0 34-35 0.0 0.0 0.0 0.04463540076830483 0.0 36-37 0.0 0.0 0.0 0.077258325016476 0.0 38-39 0.0 0.0 0.0 0.1249379835330339 0.0 40-41 0.0 0.0 0.0 0.17880900409164696 0.0 42-43 0.0 0.0 0.0 0.23739039643640833 0.0 44-45 0.0 0.0 0.0 0.310164612066944 0.0 46-47 0.0 0.0 0.0 0.39980566116753047 0.0 48-49 0.0 0.0 0.0 0.4984972062764367 0.0 50-51 0.0 0.0 0.0 0.6081727624499857 0.0 52-53 0.0 0.0 0.0 0.7186505216788177 0.0 54-55 0.0 0.0 0.0 0.8447403865220013 0.0 56-57 0.0 0.0 0.0 0.9944232489141462 0.0 58-59 0.0 0.0 0.0 1.159265692120227 0.0 60-61 0.0 0.0 0.0 1.344039795853721 0.0 62-63 0.0 0.0 0.0 1.5428010297818913 0.0 64-65 0.0 0.0 0.0 1.7514766707009937 0.0 66-67 0.0 0.0 0.0 1.983354492295971 0.0 68-69 0.0 0.0 0.0 2.2510640503602506 0.0 70-71 0.0 0.0 0.0 2.552836384310389 0.0 72-73 0.0 0.0 0.0 2.866106962052916 2.0569309109817897E-5 74-75 0.0 0.0 0.0 3.1942697195909506 4.1138618219635794E-5 76-77 0.0 0.0 0.0 3.5550348320680465 4.1138618219635794E-5 78-79 0.0 0.0 0.0 3.9533800722887804 4.1138618219635794E-5 80-81 0.0 0.0 0.0 4.398541060043459 4.1138618219635794E-5 82-83 0.0 0.0 0.0 4.858717643448305 4.1138618219635794E-5 84-85 0.0 0.0 0.0 5.336254723741837 4.1138618219635794E-5 86-87 0.0 0.0 0.0 5.848286535412534 4.1138618219635794E-5 88-89 0.0 0.0 0.0 6.40330820312275 4.1138618219635794E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 15445 0.0 25.51403 1 GTACATG 12845 0.0 23.935091 1 TAGGTCG 160 1.875289E-6 23.72909 5 TACATGG 12840 0.0 23.507324 2 GGTATCA 11460 0.0 22.176516 1 ACATGGG 13125 0.0 22.165232 3 TCAACGC 18015 0.0 21.733555 4 ATCAACG 18045 0.0 21.671124 3 TATCAAC 18375 0.0 21.46272 2 CAACGCA 18260 0.0 21.41596 5 CATGGGG 5175 0.0 20.909111 4 AACGCAG 18750 0.0 20.856289 6 GAGTACT 7835 0.0 20.656334 12-13 AGTACTT 8200 0.0 20.084152 12-13 GTACCGT 170 8.012547E-5 19.541605 6 GTACTTT 8475 0.0 19.040445 14-15 ACGCAGA 20735 0.0 18.699461 7 CGCAGAG 20890 0.0 18.560715 8 GGTACGT 130 0.0076040123 18.253147 2 CATGGGA 8075 0.0 18.101696 4 >>END_MODULE