FastQCFastQC Report
Fri 27 May 2016
SRR939279_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939279_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2261719
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74320.3285996182549645No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56220.24857199324938242No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39680.17544177680781742No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28850.12755784427685313No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA144100.026.2825221
TAGGACG1801.9860454E-723.7084664
GGTATCA105100.023.7074411
GTACATG118000.023.1267151
TACATGG117950.022.592892
GAGTACT71700.022.10732812-13
ATCAACG173550.021.6934853
GTCCCGA3950.021.5947976
TATCAAC174350.021.5939462
TCAACGC174100.021.5704824
ACATGGG120700.021.4102973
CAACGCA175800.021.361425
AGTACTT74900.021.00465612-13
AACGCAG180250.020.8483496
GTACTTT76900.020.33737814-15
CCGAGCG4600.019.5726789
CATGGGG54500.019.401794
ACGCAGA196450.019.0326257
GTGTAGC14050.018.9164561
CGCAGAG198200.018.7446168
Produced by FastQC (version 0.11.5)