##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939279_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2261719 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.964558815661892 33.0 31.0 34.0 30.0 34.0 2 32.04087554643172 34.0 31.0 34.0 30.0 34.0 3 32.088493751876335 34.0 31.0 34.0 30.0 34.0 4 35.57160637550465 37.0 35.0 37.0 33.0 37.0 5 35.60714527313075 37.0 35.0 37.0 33.0 37.0 6 35.70083374636725 37.0 35.0 37.0 33.0 37.0 7 35.643515396917124 37.0 35.0 37.0 33.0 37.0 8 35.627404200079674 37.0 35.0 37.0 33.0 37.0 9 37.26579473400542 39.0 37.0 39.0 34.0 39.0 10-11 37.16167835173158 39.0 37.0 39.0 34.0 39.0 12-13 37.06250223834172 39.0 37.0 39.0 33.0 39.0 14-15 38.29744565969513 40.0 38.0 41.0 33.0 41.0 16-17 38.222737218902964 40.0 38.0 41.0 33.0 41.0 18-19 38.15567163736963 40.0 38.0 41.0 33.0 41.0 20-21 38.132030990587246 40.0 38.0 41.0 33.0 41.0 22-23 38.1489486536568 40.0 38.0 41.0 33.0 41.0 24-25 38.07122723910442 40.0 38.0 41.0 33.0 41.0 26-27 37.98793815677368 40.0 38.0 41.0 33.0 41.0 28-29 37.847347968514214 40.0 38.0 41.0 33.0 41.0 30-31 37.67192210880308 40.0 37.5 41.0 32.5 41.0 32-33 37.45671013950009 40.0 37.0 41.0 32.0 41.0 34-35 37.17968434628705 40.0 37.0 41.0 31.0 41.0 36-37 36.95108477224625 40.0 36.5 41.0 30.5 41.0 38-39 36.37184592780977 39.5 35.5 40.5 29.5 41.0 40-41 36.34820594423976 39.0 35.5 40.0 30.0 41.0 42-43 36.52322304406516 39.5 36.0 41.0 30.0 41.0 44-45 36.42224675125425 40.0 36.0 41.0 30.0 41.0 46-47 36.13906347340231 40.0 36.0 41.0 29.0 41.0 48-49 35.888909055457376 39.0 35.0 41.0 28.0 41.0 50-51 34.77565161719913 38.0 34.0 40.0 25.5 40.5 52-53 34.87944811004373 38.5 34.5 39.5 26.5 40.5 54-55 35.36514770402512 39.0 35.0 40.5 27.0 41.0 56-57 35.28273339879976 39.0 35.0 41.0 26.5 41.0 58-59 35.073904848480296 38.5 35.0 40.5 26.0 41.0 60-61 34.766105338461585 38.0 34.5 40.0 26.0 41.0 62-63 34.38081410643851 38.0 34.0 40.0 25.0 41.0 64-65 33.99080411846034 37.0 34.0 40.0 23.5 41.0 66-67 33.6250365319476 36.5 33.5 39.5 22.5 41.0 68-69 33.21979034530815 36.0 33.0 39.0 22.0 41.0 70-71 32.80444277118421 35.5 33.0 39.0 21.5 40.0 72-73 32.36128073381353 35.0 32.0 38.0 20.0 40.0 74-75 31.895715824998597 35.0 32.0 37.0 18.5 39.5 76-77 31.42791257446217 35.0 31.0 37.0 17.5 39.0 78-79 31.024898539562166 35.0 31.0 36.0 14.0 39.0 80-81 30.61657659505889 35.0 31.0 36.0 8.5 37.5 82-83 30.18580137497187 34.0 30.5 35.0 7.0 37.0 84-85 29.768507493636477 34.0 30.0 35.0 2.0 36.5 86-87 29.340520197248196 34.0 29.5 35.0 2.0 36.0 88-89 28.910082773324184 34.0 29.0 35.0 2.0 36.0 90-91 28.50135096358124 34.0 29.0 35.0 2.0 35.5 92-93 28.05607261556365 34.0 28.5 35.0 2.0 35.0 94-95 27.564781036017294 34.0 27.0 35.0 2.0 35.0 96-97 26.952751867053337 33.0 25.5 35.0 2.0 35.0 98-99 26.145713282684543 33.0 24.5 35.0 2.0 35.0 100-101 23.318695646983556 30.0 10.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.22315244834925707 1101 2 0.36153104112151624 1101 3 0.3905074261247208 1101 4 0.28887107213457597 1101 5 0.22750068180446448 1101 6 0.35163666900802326 1101 7 0.25122421276650186 1101 8 0.17119412527600275 1101 9 0.313557874747012 1101 10-11 0.3301054236748513 1101 12-13 0.3865047320501773 1101 14-15 0.5091567944742081 1101 16-17 0.5582561396277015 1101 18-19 0.5179468425353306 1101 20-21 0.6043200845441774 1101 22-23 0.5123206811837733 1101 24-25 0.585854898068348 1101 26-27 0.5623555970218419 1101 28-29 0.6324789073220956 1101 30-31 0.5977717129313973 1101 32-33 0.6198121965256433 1101 34-35 0.4966614890835075 1101 36-37 0.3777349671918486 1101 38-39 0.8665401822788468 1101 40-41 0.7757092914709531 1101 42-43 0.7620483423447908 1101 44-45 0.5693353548519227 1101 46-47 0.7566272577008633 1101 48-49 0.7255754834684893 1101 50-51 0.5902109161430005 1101 52-53 0.6345795797804854 1101 54-55 0.6744882862524051 1101 56-57 0.6441699563083532 1101 58-59 0.5758330521821051 1101 60-61 0.5792318355205737 1101 62-63 0.6548657087416387 1101 64-65 0.4217791773487676 1101 66-67 0.4554678628139257 1101 68-69 0.596181628514806 1101 70-71 0.7566624377137643 1101 72-73 0.6795846097970824 1101 74-75 0.522400232847172 1101 76-77 0.5877954591390839 1101 78-79 0.5087482166078559 1101 80-81 0.5338396336144591 1101 82-83 0.3238063815999297 1101 84-85 0.36546366654432205 1101 86-87 0.31228749859669946 1101 88-89 0.12622613317848774 1101 90-91 0.22835233131982235 1101 92-93 0.25290567428308464 1101 94-95 0.40339028824812573 1101 96-97 0.46071500455638414 1101 98-99 0.7116477941099824 1101 100-101 0.5865123144817055 1102 1 0.34859360048276145 1102 2 0.2341270746971844 1102 3 0.3091016277403895 1102 4 0.26094811451981315 1102 5 0.22518282904632514 1102 6 0.3028785339125193 1102 7 0.3668907421644576 1102 8 0.37922309410573973 1102 9 0.3475467944679096 1102 10-11 0.4863442385198766 1102 12-13 0.4381132776047849 1102 14-15 0.6550160260516549 1102 16-17 0.4954080768484346 1102 18-19 0.5522992196573782 1102 20-21 0.5604632121925377 1102 22-23 0.5641890109827585 1102 24-25 0.6149007009171257 1102 26-27 0.4861423564924152 1102 28-29 0.5709889388425822 1102 30-31 0.7017869189691694 1102 32-33 0.5285350779370219 1102 34-35 0.50126881076568 1102 36-37 0.4674488100358474 1102 38-39 0.8088115529005151 1102 40-41 0.6860943109643927 1102 42-43 0.4771672561772391 1102 44-45 0.5087738745050885 1102 46-47 0.6743689821294296 1102 48-49 0.7108098762083799 1102 50-51 0.7096837658029713 1102 52-53 0.7939158406577462 1102 54-55 0.7421603981251224 1102 56-57 0.6742847232145337 1102 58-59 0.6424369216203161 1102 60-61 0.6078343213671431 1102 62-63 0.6386491583685086 1102 64-65 0.606117025796415 1102 66-67 0.6981171714029699 1102 68-69 0.7246509508820296 1102 70-71 0.6452097049759473 1102 72-73 0.6534091823076764 1102 74-75 0.6452752939445752 1102 76-77 0.6010391637009 1102 78-79 0.5736651722444037 1102 80-81 0.5184445306436807 1102 82-83 0.4031416033402131 1102 84-85 0.32161834015909463 1102 86-87 0.2859939654924908 1102 88-89 0.27030921025550825 1102 90-91 0.4762297978268961 1102 92-93 0.3016967634910692 1102 94-95 0.20399962662576243 1102 96-97 0.14752681684975855 1102 98-99 0.5096395656845836 1102 100-101 0.27196098111501854 1103 1 0.38397391856757324 1103 2 0.4277418231805541 1103 3 0.46253779849834586 1103 4 0.3664724485676416 1103 5 0.3697707466754565 1103 6 0.41616245613421654 1103 7 0.4236242677322153 1103 8 0.3461289489181283 1103 9 0.5190177545476828 1103 10-11 0.542120274914339 1103 12-13 0.5141191819254516 1103 14-15 0.585302915478934 1103 16-17 0.5804559419038142 1103 18-19 0.6419981829925732 1103 20-21 0.783487077251678 1103 22-23 0.6542209680360926 1103 24-25 0.6775259702623728 1103 26-27 0.6215142351317766 1103 28-29 0.6359777230781702 1103 30-31 0.7328983821809487 1103 32-33 0.6826045597268546 1103 34-35 0.6133970696493165 1103 36-37 0.45181021200875193 1103 38-39 0.8858866103663274 1103 40-41 0.8141246948486724 1103 42-43 0.7705490618612458 1103 44-45 0.7235203831840806 1103 46-47 0.6114872382720122 1103 48-49 0.4154122419800572 1103 50-51 0.3883626940357132 1103 52-53 0.41754278519766075 1103 54-55 0.5606774112478732 1103 56-57 0.5722517101726439 1103 58-59 0.49992454337518666 1103 60-61 0.6160775908506295 1103 62-63 0.5102976524094203 1103 64-65 0.4536415178193707 1103 66-67 0.5540092913117434 1103 68-69 0.5655550623259629 1103 70-71 0.5616532004202881 1103 72-73 0.5874403410223792 1103 74-75 0.6315763578789095 1103 76-77 0.49624581727505657 1103 78-79 0.7761706061658984 1103 80-81 0.5931875742133172 1103 82-83 0.6846443606175328 1103 84-85 0.6799455574411972 1103 86-87 0.627500357138242 1103 88-89 0.5739404756034254 1103 90-91 0.45500764858459064 1103 92-93 0.47265771307208126 1103 94-95 0.6306432145043708 1103 96-97 0.7949084495133754 1103 98-99 0.8855980539565742 1103 100-101 0.36991555574110535 1104 1 0.1507660324050768 1104 2 0.2480382862302548 1104 3 0.232483596583549 1104 4 0.24486878200305995 1104 5 0.15128818651216847 1104 6 0.06969798899113755 1104 7 0.1307440840322016 1104 8 0.19743315489934332 1104 9 0.21358859370374716 1104 10-11 0.3000025104145294 1104 12-13 0.39800959953068826 1104 14-15 0.3805575408592645 1104 16-17 0.4265131859667264 1104 18-19 0.44884559551687175 1104 20-21 0.48644345872199324 1104 22-23 0.5276563832688623 1104 24-25 0.32766824854596166 1104 26-27 0.27983606242830206 1104 28-29 0.31994728486662893 1104 30-31 0.3367827907768586 1104 32-33 0.33768045309645345 1104 34-35 0.32256822138538865 1104 36-37 0.17407280153392435 1104 38-39 0.7744158855812842 1104 40-41 0.550432820860344 1104 42-43 0.4000356610268625 1104 44-45 0.44830171426561805 1104 46-47 0.46946397741083246 1104 48-49 0.48154689676874796 1104 50-51 0.4193326773378203 1104 52-53 0.447138782723421 1104 54-55 0.5265562997345086 1104 56-57 0.535238443413995 1104 58-59 0.6051149704145189 1104 60-61 0.7009590302616431 1104 62-63 0.6935780138236751 1104 64-65 0.5772815321517655 1104 66-67 0.5096656605595555 1104 68-69 0.48332156049561803 1104 70-71 0.5426763725172989 1104 72-73 0.557122727932871 1104 74-75 0.4452557072886201 1104 76-77 0.4310893111631131 1104 78-79 0.3476370368097079 1104 80-81 0.43517804809782845 1104 82-83 0.24431561250376888 1104 84-85 0.3493400184637352 1104 86-87 0.39038545307558437 1104 88-89 0.25086711131571704 1104 90-91 0.22351718655079367 1104 92-93 0.1155574620693045 1104 94-95 0.15508078191983188 1104 96-97 0.3114275321844211 1104 98-99 0.16965317325609064 1104 100-101 0.11737892192957844 1105 1 0.16309186691525568 1105 2 0.19935380348714205 1105 3 0.1980764113856992 1105 4 0.1758331348585216 1105 5 0.1566554969202727 1105 6 0.19868794158753644 1105 7 0.227846797820618 1105 8 0.21206350579389976 1105 9 0.22322042877161152 1105 10-11 0.25498213676264925 1105 12-13 0.2783031510225058 1105 14-15 0.3151225881279345 1105 16-17 0.3229379023996728 1105 18-19 0.3507028360339035 1105 20-21 0.3593743861953911 1105 22-23 0.37868136235550764 1105 24-25 0.3659572215879834 1105 26-27 0.3748186571230505 1105 28-29 0.34645327103233825 1105 30-31 0.24792870750123797 1105 32-33 0.31725687902721234 1105 34-35 0.20728078648663484 1105 36-37 0.19553214867757163 1105 38-39 0.6601592765125233 1105 40-41 0.5457556510439261 1105 42-43 0.4163800072260315 1105 44-45 0.23293939607685132 1105 46-47 0.3519358905552039 1105 48-49 0.23300363536534974 1105 50-51 0.231022116320311 1105 52-53 0.34107638447923705 1105 54-55 0.5978033651190842 1105 56-57 0.47939214979953704 1105 58-59 0.4520215934254992 1105 60-61 0.5419100705322109 1105 62-63 0.3558653947524988 1105 64-65 0.40025383657819447 1105 66-67 0.35694130302670146 1105 68-69 0.36837188746348914 1105 70-71 0.32360676928629317 1105 72-73 0.32365529429340967 1105 74-75 0.24459187704206542 1105 76-77 0.33574714175147236 1105 78-79 0.3509035673521943 1105 80-81 0.19988170815917172 1105 82-83 0.21894232042745543 1105 84-85 0.510327173618311 1105 86-87 0.3949229756754953 1105 88-89 0.1707091592949972 1105 90-91 0.24292050390302933 1105 92-93 0.1822539621187751 1105 94-95 0.0731937679865915 1105 96-97 -0.04968172995656417 1105 98-99 0.037827100378684975 1105 100-101 -0.14501819787469117 1106 1 0.17828141987827095 1106 2 0.25508469524048394 1106 3 0.2831389628086711 1106 4 0.2086067050363667 1106 5 0.23110751270052532 1106 6 0.21949973880700924 1106 7 0.14441606851111288 1106 8 0.15525246805207615 1106 9 0.13712941803344592 1106 10-11 0.3789869045945551 1106 12-13 0.371700442218895 1106 14-15 0.4609644480043045 1106 16-17 0.4363347716640291 1106 18-19 0.5676070152706316 1106 20-21 0.5044831391752851 1106 22-23 0.5476957291410187 1106 24-25 0.5101524917299614 1106 26-27 0.5699474518742207 1106 28-29 0.5328871015422436 1106 30-31 0.4575703817275212 1106 32-33 0.29490186541990226 1106 34-35 0.19638333706573263 1106 36-37 0.3102480829405607 1106 38-39 0.787040565936806 1106 40-41 0.7159920384619127 1106 42-43 0.5611026924014482 1106 44-45 0.49557889735319804 1106 46-47 0.44751404291486097 1106 48-49 0.5095821885604366 1106 50-51 0.5053707587540117 1106 52-53 0.5268345963313124 1106 54-55 0.5888858317337125 1106 56-57 0.6512872574976925 1106 58-59 0.7302593943157802 1106 60-61 0.6939068638488095 1106 62-63 0.5948735380406163 1106 64-65 0.603499548693982 1106 66-67 0.468067123157077 1106 68-69 0.5684963574113837 1106 70-71 0.4917419352110599 1106 72-73 0.559900837636377 1106 74-75 0.709286714703719 1106 76-77 0.8191151086720048 1106 78-79 0.8681437099652207 1106 80-81 0.7514586791091524 1106 82-83 0.7978367530343604 1106 84-85 0.8072750228198018 1106 86-87 0.8777830419188213 1106 88-89 0.9325738878872194 1106 90-91 0.7923598617224812 1106 92-93 0.7133334752092182 1106 94-95 0.8828523194939457 1106 96-97 0.9379292909079773 1106 98-99 0.9124204633879032 1106 100-101 0.5022250037455258 1107 1 0.09305949531084323 1107 2 0.13602721596953415 1107 3 0.15126495208707524 1107 4 0.12139523307039468 1107 5 0.13536794648248218 1107 6 0.10080560135740768 1107 7 0.12154119159064436 1107 8 0.11325115721114543 1107 9 0.32938682463602476 1107 10-11 0.2943601780935836 1107 12-13 0.31281849117367955 1107 14-15 0.30527127310942603 1107 16-17 0.3595347491810017 1107 18-19 0.24057839858922137 1107 20-21 0.2553360389340966 1107 22-23 0.12727471433333193 1107 24-25 0.280697313757706 1107 26-27 0.24863173292413165 1107 28-29 0.2800987541754125 1107 30-31 0.35048591880595126 1107 32-33 0.3867646145524475 1107 34-35 0.41906068450984435 1107 36-37 0.3418486839601229 1107 38-39 0.8621784501431762 1107 40-41 0.633637804275061 1107 42-43 0.5381296529668163 1107 44-45 0.4488597635922176 1107 46-47 0.5314432936377855 1107 48-49 0.5166096409420717 1107 50-51 0.46336754087036525 1107 52-53 0.6565115934817882 1107 54-55 0.6364664469456969 1107 56-57 0.5497582107279655 1107 58-59 0.5287154926870627 1107 60-61 0.3754173821930564 1107 62-63 0.3309146378221044 1107 64-65 0.44099863281162044 1107 66-67 0.2999599031937521 1107 68-69 0.5799832786113726 1107 70-71 0.4471118161814829 1107 72-73 0.46627537881695247 1107 74-75 0.5348014405864099 1107 76-77 0.5654607631740767 1107 78-79 0.5785773413659818 1107 80-81 0.3922604600863018 1107 82-83 0.4839482628400731 1107 84-85 0.6655137986188322 1107 86-87 0.8039273930073918 1107 88-89 0.8012371613561378 1107 90-91 0.729598383583717 1107 92-93 0.7825307206365792 1107 94-95 1.0116103228001556 1107 96-97 0.9290724827153163 1107 98-99 1.2136768030502765 1107 100-101 0.9443765694777753 1108 1 0.06966732992725255 1108 2 0.08943438753818356 1108 3 0.10634544315488625 1108 4 0.07954945292515703 1108 5 -0.006957830003280208 1108 6 0.06334421679551383 1108 7 0.15933731758367742 1108 8 0.10041100312541573 1108 9 0.23566736470363026 1108 10-11 0.30078141810609793 1108 12-13 0.3167730725569413 1108 14-15 0.29963358389910155 1108 16-17 0.3887664759234539 1108 18-19 0.3514420663706588 1108 20-21 0.29166178363201567 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2316 82-83 -1.2888358166464293 2316 84-85 -1.2565286819960342 2316 86-87 -1.1876861060641808 2316 88-89 -1.3724689804596473 2316 90-91 -1.303261844642492 2316 92-93 -1.4502026514800406 2316 94-95 -1.4653743886233208 2316 96-97 -1.5075021727508506 2316 98-99 -1.842882496401728 2316 100-101 -1.0697283408596512 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7736.0 3 1689.0 4 1748.0 5 2059.0 6 2444.0 7 2819.0 8 3595.0 9 4591.0 10 5280.0 11 6535.0 12 8172.0 13 11167.0 14 11470.0 15 12147.0 16 15468.0 17 19631.0 18 17381.0 19 12394.0 20 12517.0 21 13298.0 22 14485.0 23 16106.0 24 18211.0 25 20769.0 26 24054.0 27 28195.0 28 33499.0 29 40044.0 30 48279.0 31 59656.0 32 74573.0 33 97260.0 34 132687.0 35 193101.0 36 305233.0 37 484258.0 38 441207.0 39 57952.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.3637626286143 19.406846480573375 15.65433760916861 22.575053281643708 2 14.879660940369552 20.39832773579005 40.854196031251746 23.867815292588652 3 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21.60269841628368 22-23 22.704729037247137 26.26036500404369 28.862411740789195 22.172494217919976 24-25 22.456677558052505 26.465535973802197 28.97536136829652 22.10242509984878 26-27 22.23536712138372 26.686564136453207 28.93835032450578 22.139718417657296 28-29 21.971157072177743 26.906756436243466 29.15221386667127 21.969872624907513 30-31 22.27342750608001 26.495745043154084 28.9814697742064 22.249357676559505 32-33 22.08087260242059 26.612433884696507 28.790374515346368 22.516318997536537 34-35 22.63845364541446 26.732832302615154 28.573637649407914 22.055076402562467 36-37 22.712374602740237 26.398971855750613 28.681333033620817 22.207320507888333 38-39 22.3660872098497 26.612979868227754 28.76622505733283 22.25470786458972 40-41 22.499194601040546 26.506029446407275 28.78761202039352 22.207163932158654 42-43 22.3213916908767 26.518747471104525 28.49533883757196 22.66452200044682 44-45 22.397740600325708 26.73916099555187 28.340257560369807 22.52284084375262 46-47 22.58019954497846 26.975239437095183 27.880707424348895 22.563853593577463 48-49 22.652037397525962 26.81217009957096 27.94319382263807 22.592598680265013 50-51 22.50967863876506 27.112959392471776 27.863330135808557 22.51403183295461 52-53 22.696903290750566 27.17549055625621 27.57816626515337 22.549439887839856 54-55 22.366842538434103 26.93234274252471 27.802692574699606 22.89812214434158 56-57 22.515670492853236 27.185394112756505 27.69024649876734 22.60868889562292 58-59 22.743225931905375 26.762638412035507 27.809756274061105 22.684379381998017 60-61 22.556594839789586 26.930241928684335 28.055171703778253 22.457991527747826 62-63 22.49496029091271 26.91186883696219 28.030466585107337 22.562704287017763 64-65 22.25590098311035 26.896840156336378 27.957110331220882 22.890148529332393 66-67 22.255499308074537 27.0090161330807 27.767224279364626 22.968260279480134 68-69 22.33152791227169 26.91785722933735 27.8821702285245 22.868444629866467 70-71 22.36902929548969 26.973171106087385 27.72852163524815 22.929277963174773 72-73 22.485187911505516 26.84418664675285 27.71636399821771 22.95426144352393 74-75 22.344255135963138 27.214218409100905 27.49136916516248 22.950157289773475 76-77 22.403949997434978 26.9508308998956 27.538742349633754 23.10647675303566 78-79 22.454012469109788 27.0434504754023 27.571268154696543 22.93126890079137 80-81 22.397632472326034 27.236649072597686 27.52546240219771 22.840256052878573 82-83 22.497571093402147 27.102822417225642 27.515497812040934 22.884108677331277 84-85 22.464624553240498 27.012943866975263 27.471573281882982 23.050858297901257 86-87 22.444940959671005 26.9789353131366 27.521603112989094 23.0545206142033 88-89 22.476119913800936 26.83635525333024 27.511942541655365 23.175582291213455 90-91 22.396304785861197 26.972228805974822 27.671434438892867 22.960031969271117 92-93 22.572372166774368 26.723182529338835 27.616126347207352 23.08831895667944 94-95 22.405564816229916 26.775457870250253 27.57191041985805 23.24706689366179 96-97 22.373074500944632 26.700009218626956 27.70808273662161 23.218833543806806 98-99 22.36287894572467 26.657822989325837 27.378474590050683 23.60082347489881 100-101 22.523646372986512 26.272153657173174 27.84816395677348 23.35603601306683 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2528.0 1 1445.5 2 393.5 3 405.0 4 366.0 5 383.0 6 569.5 7 1177.5 8 1973.5 9 2425.0 10 2472.5 11 2376.0 12 2507.0 13 2780.0 14 2979.5 15 3221.0 16 3339.5 17 3427.5 18 3522.5 19 3614.0 20 3832.0 21 4183.5 22 4934.0 23 5983.0 24 7017.5 25 8496.5 26 10778.5 27 13557.5 28 16234.0 29 19550.0 30 23469.5 31 27511.5 32 32022.5 33 36212.5 34 41414.0 35 46392.5 36 51186.0 37 57044.0 38 61014.5 39 62923.0 40 65823.0 41 71433.0 42 76068.5 43 79363.0 44 82285.5 45 85504.5 46 86655.5 47 89524.0 48 100933.5 49 100966.5 50 91155.5 51 85305.0 52 79642.0 53 76972.0 54 74166.5 55 67526.0 56 59770.5 57 54078.0 58 48903.5 59 42279.5 60 35504.0 61 29321.5 62 23867.5 63 18467.0 64 14301.5 65 11269.0 66 8409.0 67 6949.0 68 5675.0 69 3924.0 70 2670.0 71 2007.5 72 1644.0 73 1353.5 74 1052.0 75 848.5 76 605.0 77 343.0 78 207.5 79 150.0 80 109.0 81 76.0 82 53.5 83 39.0 84 23.5 85 16.5 86 18.0 87 12.0 88 8.0 89 8.0 90 8.0 91 8.5 92 5.0 93 2.5 94 2.5 95 2.5 96 1.5 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29716335229973306 2 0.18485939234714835 3 0.18490360650461 4 0.20709911355035704 5 0.22031914663138966 6 0.11438202535328218 7 0.1296801238350122 8 0.14117580477504058 9 0.14608357625328347 10-11 0.13292986440844332 12-13 0.12236268077510956 14-15 0.13834609869749515 16-17 0.1124587095037005 18-19 0.14223694455412011 20-21 0.16810222666918392 22-23 0.1715951451086541 24-25 0.1958908246338294 26-27 0.1758839183824339 28-29 0.17413745916269882 30-31 0.16383556047413494 32-33 0.17336371140711998 34-35 0.35510600565322215 36-37 0.47189770258816416 38-39 0.44015193753070125 40-41 0.43018164502309963 42-43 0.4528414007221941 44-45 0.45648906871277994 46-47 0.4596945951287494 48-49 0.47136713269862435 50-51 0.46418233211110665 52-53 0.4709692052814695 54-55 0.45399096881619694 56-57 0.41899546318530284 58-59 0.4090030635989705 60-61 0.3645457282712839 62-63 0.46853742662107895 64-65 0.4663488258267273 66-67 0.4754790493425576 68-69 0.4862451966844688 70-71 0.47943621643537504 72-73 0.47247248663516556 74-75 0.47654018912163715 76-77 0.4542783608396976 78-79 0.4418983967504363 80-81 0.4385381207833511 82-83 0.4506085857703808 84-85 0.452222402517731 86-87 0.4531730069031564 88-89 0.4606451995141749 90-91 0.4778445067667557 92-93 0.48217749419799716 94-95 0.4802983925058772 96-97 0.47934778812045176 98-99 0.4761864758619439 100-101 0.4584787057985541 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2261719.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.37054795595125 #Duplication Level Percentage of deduplicated Percentage of total 1 71.87760649347291 36.2051442483847 2 14.718241354129674 14.827317639109072 3 5.529386605388512 8.355546995211496 4 2.726451257060309 5.493313751732795 5 1.4341090083870358 3.6118428290510436 6 0.8310644331986854 2.5116702532151893 7 0.5907528484958278 2.0829581279691727 8 0.3846283253754142 1.5499151606831618 9 0.2699391681403276 1.2237285432601763 >10 1.4711791060875767 13.5027386314812 >50 0.11237311804440699 3.8712741643523043 >100 0.05057100168075616 4.556966739498987 >500 0.002640914670313084 0.8608003685633454 >1k 8.803048901042643E-4 0.7679493938673698 >5k 1.7606097802085285E-4 0.5788331536199923 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7432 0.3285996182549645 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5622 0.24857199324938242 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3968 0.17544177680781742 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2885 0.12755784427685313 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.2107078730823768E-5 0.0 18-19 0.0 0.0 0.0 4.4214157461647535E-5 0.0 20-21 0.0 0.0 0.0 1.326424723849426E-4 0.0 22-23 0.0 0.0 0.0 1.7685662984659014E-4 0.0 24-25 0.0 0.0 0.0 2.87392023500709E-4 0.0 26-27 0.0 0.0 0.0 6.189982044630655E-4 0.0 28-29 0.0 0.0 0.0 0.0013485318025802497 0.0 30-31 0.0 0.0 0.0 0.004487736982357225 0.0 32-33 0.0 0.0 0.0 0.012092572065760601 0.0 34-35 0.0 0.0 0.0 0.023322968061019075 0.0 36-37 0.0 0.0 0.0 0.04244559116318163 0.0 38-39 0.0 0.0 0.0 0.07474403318891515 0.0 40-41 0.0 0.0 0.0 0.11049117949665718 0.0 42-43 0.0 0.0 0.0 0.15112399020391126 0.0 44-45 0.0 0.0 0.0 0.20281034027657724 0.0 46-47 0.0 0.0 0.0 0.26291948734568704 0.0 48-49 0.0 0.0 0.0 0.32990393590008305 0.0 50-51 0.0 0.0 0.0 0.40637232123000244 0.0 52-53 0.0 0.0 0.0 0.48197853048941974 0.0 54-55 0.0 0.0 0.0 0.5709816294597163 0.0 56-57 0.0 0.0 0.0 0.6788641736661363 0.0 58-59 0.0 0.0 0.0 0.7990161465681634 0.0 60-61 0.0 0.0 0.0 0.9335156135664953 0.0 62-63 0.0 0.0 0.0 1.0714637848468356 0.0 64-65 0.0 0.0 0.0 1.2239805210107888 0.0 66-67 0.0 0.0 0.0 1.40194250479392 0.0 68-69 0.0 0.0 0.0 1.6001987868519476 0.0 70-71 0.0 0.0 0.0 1.8279901260943556 0.0 72-73 0.0 0.0 0.0 2.067166610883138 0.0 74-75 0.0 0.0 0.0 2.3210000888704565 0.0 76-77 0.0 0.0 0.0 2.5972280376120995 0.0 78-79 0.0 0.0 0.0 2.913315049305418 0.0 80-81 0.0 0.0 0.0 3.2596666517812336 0.0 82-83 0.0 0.0 0.0 3.6243229154461716 0.0 84-85 0.0 0.0 0.0 4.009140834913621 0.0 86-87 0.0 0.0 0.0 4.414275159734697 0.0 88-89 0.0 0.0 0.0 4.866585990567351 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 14410 0.0 26.282522 1 TAGGACG 180 1.9860454E-7 23.708466 4 GGTATCA 10510 0.0 23.707441 1 GTACATG 11800 0.0 23.126715 1 TACATGG 11795 0.0 22.59289 2 GAGTACT 7170 0.0 22.107328 12-13 ATCAACG 17355 0.0 21.693485 3 GTCCCGA 395 0.0 21.594797 6 TATCAAC 17435 0.0 21.593946 2 TCAACGC 17410 0.0 21.570482 4 ACATGGG 12070 0.0 21.410297 3 CAACGCA 17580 0.0 21.36142 5 AGTACTT 7490 0.0 21.004656 12-13 AACGCAG 18025 0.0 20.848349 6 GTACTTT 7690 0.0 20.337378 14-15 CCGAGCG 460 0.0 19.572678 9 CATGGGG 5450 0.0 19.40179 4 ACGCAGA 19645 0.0 19.032625 7 GTGTAGC 1405 0.0 18.916456 1 CGCAGAG 19820 0.0 18.744616 8 >>END_MODULE