FastQCFastQC Report
Fri 27 May 2016
SRR939279_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939279_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2261719
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74740.33045661286835365No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60370.26692086859596614No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43960.19436543620140256No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT31330.13852295532734174No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA145050.026.804891
GGTATCA104750.024.2149121
GAGTACT75550.022.55110512-13
TATCAAC176300.021.9109272
ATCAACG175550.021.8964063
GTACATG116750.021.7667751
TCAACGC176500.021.7247754
CAACGCA177450.021.6352125
TACATGG116150.021.4092142
AGTACTT80650.021.21332612-13
AACGCAG181900.021.1059286
GTACTTT81950.020.41352714-15
ACATGGG119650.020.188023
GTGTAGC13350.019.2496241
ACGCAGA200500.019.1006377
CGCAGAG201550.018.9775818
CATGGGG53250.018.8040544
AGAGTAC164400.018.59033210-11
GCAGAGT209250.018.0982119
TACTTTT94600.018.08514414-15
Produced by FastQC (version 0.11.5)