##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939279_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2261719 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.30502330307169 33.0 31.0 34.0 31.0 34.0 2 32.518830146450554 34.0 31.0 34.0 31.0 34.0 3 32.6060209071065 34.0 31.0 34.0 31.0 34.0 4 36.128986845846015 37.0 35.0 37.0 35.0 37.0 5 36.073896447790375 37.0 35.0 37.0 35.0 37.0 6 36.10346820272545 37.0 35.0 37.0 35.0 37.0 7 36.05013133815474 37.0 35.0 37.0 35.0 37.0 8 36.02320712696847 37.0 35.0 37.0 35.0 37.0 9 37.72804092816128 39.0 38.0 39.0 35.0 39.0 10-11 37.65112089521289 39.0 37.0 39.0 35.0 39.0 12-13 37.57389047887912 39.0 37.0 39.0 35.0 39.0 14-15 38.855344983174305 40.0 38.0 41.0 35.0 41.0 16-17 38.86071567688117 40.0 38.0 41.0 35.0 41.0 18-19 38.975746544995204 40.0 38.5 41.0 35.0 41.0 20-21 38.96284684348498 40.0 38.0 41.0 35.0 41.0 22-23 38.88536816465706 40.0 38.0 41.0 35.0 41.0 24-25 38.82000283854891 40.0 38.0 41.0 35.0 41.0 26-27 38.6668407967568 40.0 38.0 41.0 34.0 41.0 28-29 38.50038797923173 40.0 38.0 41.0 34.0 41.0 30-31 38.31188158210635 40.0 38.0 41.0 34.0 41.0 32-33 38.262151708501364 40.0 38.0 41.0 34.0 41.0 34-35 38.170209031272236 40.0 38.0 41.0 33.5 41.0 36-37 38.022962843748495 40.0 38.0 41.0 33.0 41.0 38-39 37.89440708593773 40.0 37.0 41.0 33.0 41.0 40-41 37.71613803483103 40.0 37.0 41.0 33.0 41.0 42-43 37.53015847680459 40.0 37.0 41.0 33.0 41.0 44-45 37.277421288851535 40.0 37.0 41.0 32.0 41.0 46-47 36.95702273359334 40.0 36.0 41.0 31.0 41.0 48-49 36.645349400168634 39.5 36.0 41.0 30.5 41.0 50-51 36.39942384531412 39.0 36.0 41.0 30.0 41.0 52-53 36.13656382601022 39.0 35.0 40.5 29.5 41.0 54-55 35.8913193902514 39.0 35.0 40.0 29.0 41.0 56-57 35.58185499613347 38.5 35.0 40.0 28.0 41.0 58-59 35.26578191189975 38.0 34.5 40.0 28.0 41.0 60-61 34.923742074059604 38.0 34.0 40.0 27.0 41.0 62-63 34.56745687682687 37.0 34.0 40.0 26.0 41.0 64-65 34.25019664246531 37.0 34.0 39.5 26.0 41.0 66-67 33.83956848750884 36.0 33.0 39.0 26.0 40.5 68-69 33.36304598405019 36.0 33.0 39.0 25.0 40.0 70-71 32.87125942701105 35.0 32.0 38.0 24.0 40.0 72-73 32.37932585789835 35.0 32.0 37.5 22.5 39.5 74-75 31.84179754425727 35.0 31.0 37.0 21.5 39.0 76-77 29.88725301418965 33.0 28.5 35.0 18.0 37.5 78-79 30.860535283118725 34.0 30.5 36.0 19.5 38.0 80-81 30.862230232845015 34.0 31.0 35.5 19.5 37.0 82-83 30.50106445584089 34.0 30.5 35.0 18.0 37.0 84-85 30.040912686323985 34.0 30.0 35.0 13.0 36.0 86-87 29.435400463099086 34.0 29.5 35.0 7.0 36.0 88-89 28.83743205942029 34.0 29.0 35.0 2.0 35.5 90-91 28.336971347899542 33.0 28.5 35.0 2.0 35.0 92-93 27.96436095730725 33.0 27.0 35.0 2.0 35.0 94-95 27.416925356332946 33.0 26.5 35.0 2.0 35.0 96-97 26.657255167419116 32.5 25.5 34.0 2.0 35.0 98-99 25.60818165298165 32.0 24.0 34.0 2.0 35.0 100-101 21.200279300832683 27.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2147272937751481 1101 2 0.25462410453258855 1101 3 0.24223412090584873 1101 4 0.1210971488412298 1101 5 0.1502782034286625 1101 6 0.16511677213262033 1101 7 0.19810612605963485 1101 8 0.1430135200908964 1101 9 0.21961015759704594 1101 10-11 0.27477442449652756 1101 12-13 0.2802539798905457 1101 14-15 0.24230210929174234 1101 16-17 0.21491802644933244 1101 18-19 0.2749176106907285 1101 20-21 0.23053637436630936 1101 22-23 0.2858656802688202 1101 24-25 0.33103756333176904 1101 26-27 0.3740873943399876 1101 28-29 0.31210751256787717 1101 30-31 0.4166621758549667 1101 32-33 0.4199467089326703 1101 34-35 0.5125349333824261 1101 36-37 0.45994789477107645 1101 38-39 0.4992886034967512 1101 40-41 0.6374952593334271 1101 42-43 0.5146014276200859 1101 44-45 0.6395936326013327 1101 46-47 0.6708035891571669 1101 48-49 0.5349646941744055 1101 50-51 0.4808704956112848 1101 52-53 0.5606350434046021 1101 54-55 0.49517304242534266 1101 56-57 0.4650670140831821 1101 58-59 0.5364196147497182 1101 60-61 0.576017103390015 1101 62-63 0.5759871083316312 1101 64-65 0.5845263671390342 1101 66-67 0.4902057937761839 1101 68-69 0.6286891280471991 1101 70-71 0.7053301950147812 1101 72-73 0.7518446048588103 1101 74-75 0.6527039911415002 1101 76-77 0.7051172880054857 1101 78-79 0.6975020614206002 1101 80-81 0.897563100830169 1101 82-83 0.9128338373097513 1101 84-85 0.8192387227594224 1101 86-87 0.8230739918424348 1101 88-89 1.0925323199980355 1101 90-91 1.1739914697192262 1101 92-93 1.060836026349591 1101 94-95 0.7855958342274398 1101 96-97 0.8924851707501809 1101 98-99 0.7071005050799286 1101 100-101 0.5865188670136128 1102 1 0.15045859604470024 1102 2 0.16277594168827392 1102 3 0.19700808936996594 1102 4 0.10468648190772711 1102 5 0.12444073599791494 1102 6 0.17901715353437453 1102 7 0.19040833403327895 1102 8 0.20699934164413492 1102 9 0.22395961936257436 1102 10-11 0.23523863007631007 1102 12-13 0.2643888598702304 1102 14-15 0.30141625464006694 1102 16-17 0.23459355592810738 1102 18-19 0.23640363429837663 1102 20-21 0.25019843355092775 1102 22-23 0.4041682981242758 1102 24-25 0.30479310058483833 1102 26-27 0.3203471747357014 1102 28-29 0.2855101762223313 1102 30-31 0.29478800572130837 1102 32-33 0.35083880802785217 1102 34-35 0.35349044869815316 1102 36-37 0.36436557395485636 1102 38-39 0.37954442362598684 1102 40-41 0.36529356861907303 1102 42-43 0.32878449276628174 1102 44-45 0.3853312466658636 1102 46-47 0.40233076451345084 1102 48-49 0.4111811098445912 1102 50-51 0.3780418781637849 1102 52-53 0.40970833527509143 1102 54-55 0.35148998497646033 1102 56-57 0.32348893063663553 1102 58-59 0.36812392447411924 1102 60-61 0.438983319892273 1102 62-63 0.4199811371426634 1102 64-65 0.3862960751415443 1102 66-67 0.14517431177441864 1102 68-69 0.20664869599713143 1102 70-71 0.18368583772100777 1102 72-73 0.22567038007423434 1102 74-75 0.11786836721672245 1102 76-77 0.4066651210200227 1102 78-79 0.38600607941691223 1102 80-81 0.34559542761180495 1102 82-83 0.49665611024869705 1102 84-85 0.35827611629598977 1102 86-87 0.34225929883043804 1102 88-89 0.2412090079221123 1102 90-91 0.4267572097579446 1102 92-93 0.5153415510945223 1102 94-95 0.4561158068250144 1102 96-97 0.2650330961324485 1102 98-99 0.001010296444320602 1102 100-101 0.11209438533128946 1103 1 0.14226034477302107 1103 2 0.1898914802823981 1103 3 0.24968574439834867 1103 4 0.1490666810410204 1103 5 0.1922499170802041 1103 6 0.24757154470243847 1103 7 0.2839401925872309 1103 8 0.2261949661090341 1103 9 0.23142229891236354 1103 10-11 0.2413653383587686 1103 12-13 0.2503797330448805 1103 14-15 0.3286816580385121 1103 16-17 0.23951864233251285 1103 18-19 0.325278752603424 1103 20-21 0.38453019167783964 1103 22-23 0.32443316976712566 1103 24-25 0.41858644560017666 1103 26-27 0.37362910816329276 1103 28-29 0.36552528729624356 1103 30-31 0.3449659158137095 1103 32-33 0.3733019553909074 1103 34-35 0.4439877506615133 1103 36-37 0.4541483440394387 1103 38-39 0.5971662208094202 1103 40-41 0.536838462002315 1103 42-43 0.5108931505089132 1103 44-45 0.5726938705952378 1103 46-47 0.6743070569592788 1103 48-49 0.5506202605025621 1103 50-51 0.6257703978944562 1103 52-53 0.5046369338052656 1103 54-55 0.5740902321887447 1103 56-57 0.7577329561439754 1103 58-59 0.6789927783127965 1103 60-61 0.6082242916838396 1103 62-63 0.6716895959118645 1103 64-65 0.6433022253580276 1103 66-67 0.6298380292584582 1103 68-69 0.5052473249046088 1103 70-71 0.5410616779630075 1103 72-73 0.7051311441755175 1103 74-75 0.6564950508155647 1103 76-77 0.6575158829444412 1103 78-79 0.5602785122386891 1103 80-81 0.6672499632612912 1103 82-83 0.6597360517790953 1103 84-85 0.6551648759419955 1103 86-87 0.548691425707041 1103 88-89 0.9065746521856148 1103 90-91 0.7842924356271261 1103 92-93 0.5966489081752187 1103 94-95 0.33043177684171 1103 96-97 0.4253844403550957 1103 98-99 0.37937578978515063 1103 100-101 -0.05718772581832354 1104 1 0.018474055445501847 1104 2 -0.0011896720160109453 1104 3 0.011911847665011521 1104 4 0.02539285731737806 1104 5 0.08881223394600113 1104 6 0.1159312524591698 1104 7 0.049857677224288466 1104 8 0.09343141202864302 1104 9 0.18528909409344863 1104 10-11 0.1360488522489689 1104 12-13 0.12050589435680337 1104 14-15 0.14463936539464584 1104 16-17 0.14241772211291703 1104 18-19 0.05567128079766093 1104 20-21 0.07004290238237587 1104 22-23 0.15356945251866705 1104 24-25 0.1910803796609386 1104 26-27 0.22207183133664188 1104 28-29 0.1806177763134187 1104 30-31 0.12542387854112036 1104 32-33 0.17350506734205595 1104 34-35 0.223786488351287 1104 36-37 0.16033915727264514 1104 38-39 0.20077167288164333 1104 40-41 0.28846537920392734 1104 42-43 0.20887362610829285 1104 44-45 0.1892765521005444 1104 46-47 0.10227725264478238 1104 48-49 0.10563232267094236 1104 50-51 0.12866743284570248 1104 52-53 0.33825476174125413 1104 54-55 0.36373656504324714 1104 56-57 0.13708698996452284 1104 58-59 0.09069159449613551 1104 60-61 0.24221474326112258 1104 62-63 0.3747429905838757 1104 64-65 0.15358457908652667 1104 66-67 0.2553403485129877 1104 68-69 0.2867892794545597 1104 70-71 0.27144014795675986 1104 72-73 0.15007783687347143 1104 74-75 0.22830828913249945 1104 76-77 0.3910473400850556 1104 78-79 0.4944400091279917 1104 80-81 0.4930227344091236 1104 82-83 0.4696710401537736 1104 84-85 0.3951911055233275 1104 86-87 0.2834358383275415 1104 88-89 0.462401160514343 1104 90-91 0.531607203150962 1104 92-93 0.4117011977100127 1104 94-95 0.2319608210839128 1104 96-97 0.3643254930424469 1104 98-99 0.36886339277599234 1104 100-101 0.17701829257863366 1105 1 0.045616437138896515 1105 2 0.10860991961145317 1105 3 0.08096098729423318 1105 4 0.04221753597136768 1105 5 0.07255019190341727 1105 6 0.06433734026825988 1105 7 0.12231007124082538 1105 8 0.09883155414948419 1105 9 0.17083009775441127 1105 10-11 0.1939028275847079 1105 12-13 0.19503455689176974 1105 14-15 0.2185446751371174 1105 16-17 0.23127270488750895 1105 18-19 0.11564200210589348 1105 20-21 0.20570398915704402 1105 22-23 0.18829955784999441 1105 24-25 0.28011683635456563 1105 26-27 0.2029291438135914 1105 28-29 0.2001108186888132 1105 30-31 0.10715707814893705 1105 32-33 0.1391379098487775 1105 34-35 0.3186941997560382 1105 36-37 0.22968580618919532 1105 38-39 0.2884984590617421 1105 40-41 0.3805814955271387 1105 42-43 0.2541732941931727 1105 44-45 0.20212364626909363 1105 46-47 0.3160775908564091 1105 48-49 0.3192610814523036 1105 50-51 0.3062699082668914 1105 52-53 0.26106493147671017 1105 54-55 0.2749627620515085 1105 56-57 0.30907074501296705 1105 58-59 0.2533385501418337 1105 60-61 0.16098256458464988 1105 62-63 0.33792469837263184 1105 64-65 0.32209424420305055 1105 66-67 0.2716453416318245 1105 68-69 0.3100903507578465 1105 70-71 0.25752534850006015 1105 72-73 0.22014093672685675 1105 74-75 0.24753148181417828 1105 76-77 0.4168087659708384 1105 78-79 0.32960052472081003 1105 80-81 0.3549303018940364 1105 82-83 0.3453234017883453 1105 84-85 0.43319831051015356 1105 86-87 0.36575573614445744 1105 88-89 0.37777067469494696 1105 90-91 0.5160004461146315 1105 92-93 0.4328189601163537 1105 94-95 0.5690190546478178 1105 96-97 0.5355811036673224 1105 98-99 0.8116662765697171 1105 100-101 0.7734148996684844 1106 1 0.11724783732032762 1106 2 0.1306463745385642 1106 3 0.13439656882205497 1106 4 0.0912681889111795 1106 5 0.07081394161333776 1106 6 0.17017793434855122 1106 7 0.065044401740181 1106 8 0.002840963233680327 1106 9 -0.012920245065366487 1106 10-11 0.10566312800030886 1106 12-13 0.10978582796199987 1106 14-15 0.18302907420042658 1106 16-17 0.10928951887877503 1106 18-19 0.07305798489107218 1106 20-21 0.06916994979078339 1106 22-23 0.24137016817466872 1106 24-25 0.11016367080953415 1106 26-27 0.19915074251404263 1106 28-29 0.25567399246152434 1106 30-31 0.19017695114740008 1106 32-33 0.2849354668623434 1106 34-35 0.30146829734891156 1106 36-37 0.13530857786068395 1106 38-39 0.2327074419945916 1106 40-41 0.24040671018505577 1106 42-43 0.25704559646528224 1106 44-45 0.3858360152064577 1106 46-47 0.13718210341943404 1106 48-49 0.23090851505075705 1106 50-51 0.26036475858650476 1106 52-53 0.38361983538168687 1106 54-55 0.1834066767368583 1106 56-57 0.27191330107718414 1106 58-59 0.30098311460250216 1106 60-61 0.3304900644264208 1106 62-63 0.3002335104230056 1106 64-65 0.2830512915227317 1106 66-67 0.33356650363262474 1106 68-69 0.2628972535658036 1106 70-71 0.15625744275967435 1106 72-73 0.14191210189215298 1106 74-75 0.2180764070267216 1106 76-77 0.3663894710192537 1106 78-79 0.40129837919645084 1106 80-81 0.4741598540131875 1106 82-83 0.5173282118134495 1106 84-85 0.3960245167083336 1106 86-87 0.38993877025765755 1106 88-89 0.5253155577142898 1106 90-91 0.6255044650171904 1106 92-93 0.5477115784155444 1106 94-95 0.7565419972017722 1106 96-97 0.6348142422087086 1106 98-99 0.502358983474636 1106 100-101 0.526942251412116 1107 1 0.05685299918308573 1107 2 0.07924796019439384 1107 3 0.05183526994530041 1107 4 0.10295294069953798 1107 5 0.10446842763019504 1107 6 0.14761360427760195 1107 7 0.1262588667594926 1107 8 0.07373898964522141 1107 9 0.12356900975267848 1107 10-11 0.1104574333700441 1107 12-13 0.07765185720801071 1107 14-15 0.15499815601958034 1107 16-17 0.15804898759675012 1107 18-19 0.16984522015088288 1107 20-21 0.22059596606890608 1107 22-23 0.16190028589942784 1107 24-25 0.19284461152359 1107 26-27 0.2657645472950989 1107 28-29 0.24252904818229837 1107 30-31 0.2670527534854301 1107 32-33 0.19702220317956431 1107 34-35 0.29684783397768655 1107 36-37 0.3225776625905894 1107 38-39 0.1759630850845184 1107 40-41 0.20896389185697473 1107 42-43 0.20623776313679087 1107 44-45 0.375404190477731 1107 46-47 0.32394993702894226 1107 48-49 0.19586459786458477 1107 50-51 0.2621844788133032 1107 52-53 0.40410950372689314 1107 54-55 0.45540536790333164 1107 56-57 0.46439543760738644 1107 58-59 0.40787576917584545 1107 60-61 0.5177665765858279 1107 62-63 0.4814908939403395 1107 64-65 0.35176173920602594 1107 66-67 0.5950479546311627 1107 68-69 0.6999284096251301 1107 70-71 0.48070532880283423 1107 72-73 0.6407001260654184 1107 74-75 0.7201149353116847 1107 76-77 0.7373957973825149 1107 78-79 0.6804519934940529 1107 80-81 0.6908881315421027 1107 82-83 0.7913496730698704 1107 84-85 0.8268514606121684 1107 86-87 0.930365967898549 1107 88-89 1.0650153691178055 1107 90-91 1.0012952604246266 1107 92-93 1.0420088181217828 1107 94-95 1.2824211689831593 1107 96-97 1.2369052643534886 1107 98-99 1.1980655277426173 1107 100-101 0.6964525529557193 1108 1 -0.01111017189401764 1108 2 0.06155298930158182 1108 3 0.03340743434424098 1108 4 0.06688285549650175 1108 5 0.020098856033328616 1108 6 0.04129918302751179 1108 7 0.06589105047148536 1108 8 0.07450519281679391 1108 9 0.13739749421327474 1108 10-11 0.03006705934737397 1108 12-13 0.05214473460038249 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-1.2174172194138286 2316 92-93 -0.8969854617948236 2316 94-95 -0.6113835115082757 2316 96-97 -0.6225945396309456 2316 98-99 -0.5363153635853664 2316 100-101 0.025679470428780604 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 5.0 4 13.0 5 37.0 6 88.0 7 220.0 8 584.0 9 1047.0 10 1877.0 11 2950.0 12 4350.0 13 5911.0 14 7436.0 15 10212.0 16 13796.0 17 19579.0 18 18451.0 19 12632.0 20 11868.0 21 12777.0 22 13612.0 23 15357.0 24 17866.0 25 20844.0 26 25062.0 27 30123.0 28 37025.0 29 44508.0 30 54496.0 31 67147.0 32 83311.0 33 108308.0 34 147613.0 35 212658.0 36 336726.0 37 528496.0 38 374635.0 39 20095.0 40 2.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.383165352263575 20.156220621051027 16.08888806110467 22.371725965580733 2 15.24026636377021 20.982624278259145 40.0524556764125 23.72465368155814 3 19.0330010049878 25.278294960602977 30.101396327306794 25.58730770710243 4 13.194654154649626 17.639193905166824 37.22619830314907 31.939953637034485 5 13.91101193384324 37.70998961409441 33.65360595193302 14.725392500129326 6 30.98864182508968 36.5557790335581 17.73062878279751 14.72495035855471 7 27.98897652626166 33.14253450583384 20.75509822396151 18.113390743942993 8 25.981609563345405 35.03525415845205 19.93611938529941 19.04701689290314 9 26.446035073322548 17.350475456942263 20.469121053499574 35.73436841623562 10-11 25.15732502578791 27.065541740596423 26.80989548215318 20.967237751462495 12-13 26.04054703524178 24.311043944893242 27.441384186099153 22.20702483376582 14-15 22.889058280260972 25.537783217166616 26.30095079259245 25.27220770997996 16-17 22.02331566298514 28.77346383702337 26.83945273872711 22.363767761264377 18-19 21.97284187190528 27.32019523220633 29.274683791119756 21.432279104768632 20-21 23.12185996580477 26.292214019513477 29.01111941846003 21.574806596221723 22-23 22.67635811522121 26.306871012712012 28.9567360047822 22.060034867284575 24-25 22.435766777393653 26.527588086760556 29.03563616877251 22.001008967073275 26-27 22.182245210661904 26.71223382771448 29.064761211230493 22.040759750393118 28-29 21.898697406707022 26.966789419905833 29.238800222308786 21.895712951078362 30-31 22.25988285901122 26.52199499584166 29.050691089388202 22.167431055758914 32-33 22.077831065662888 26.627003619813074 28.851196810921248 22.44396850360279 34-35 22.604256662531093 26.75690350262062 28.69013626166059 21.948703573187693 36-37 22.709557518132563 26.392204600495507 28.772884305775847 22.125353575596087 38-39 22.299655804618883 26.656847883062447 28.878940897835054 22.16455541448362 40-41 22.466329257316968 26.527975550534133 28.85183767829117 22.153857513857727 42-43 22.350809529673175 26.562680656643444 28.5149606636912 22.571549149992183 44-45 22.430363773510155 26.746664650468105 28.367272675822687 22.455698900199057 46-47 22.62286978434713 26.99632380493816 27.935280043206177 22.445526367508528 48-49 22.650863274378636 26.895464203278184 27.956918409246818 22.496754113096358 50-51 22.57230009563522 27.142054340083803 27.777035962469256 22.50860960181172 52-53 22.714746047031504 27.188203834430286 27.541718286718325 22.555331831819885 54-55 22.359851069032004 27.004902023637772 27.745091233703214 22.890155673627007 56-57 22.546219135751294 27.256594988235612 27.60969245016522 22.587493425847875 58-59 22.730425074475082 26.874080460723032 27.680986884647908 22.71450758015398 60-61 22.576911173011453 27.029491090772428 27.936507936507937 22.457089799708186 62-63 22.499935888422865 27.0005022810457 27.921100313837222 22.57846151669421 64-65 22.189255647862456 26.956051338838144 27.91609565911938 22.93859735418002 66-67 22.241042785979904 27.079526756408477 27.6866557413073 22.99277471630432 68-69 22.32964012741881 26.946991290306677 27.819486040327185 22.903882541947333 70-71 22.33853984513549 27.020863334481426 27.650561365050212 22.99003545533287 72-73 22.50160165785405 26.89051999828449 27.604644962526294 23.00323338133517 74-75 22.272329144336673 27.271358643580392 27.369889893483673 23.086422318599258 76-77 22.336359467605455 26.89794603934425 27.59399164479311 23.17170284825718 78-79 22.3917713843067 26.992346964717512 27.582938095261255 23.03294355571453 80-81 22.30399395856951 27.252471132538304 27.49494189367719 22.948593015214996 82-83 22.43481617300823 27.106240872539868 27.448767950395254 23.01017500405665 84-85 22.317383370790093 27.042859877818596 27.405747575185067 23.23400917620624 86-87 22.296776043909645 26.958878305295244 27.45552183421286 23.288823816582255 88-89 22.328639556021905 26.857528552400556 27.455036057022923 23.358795834554613 90-91 22.221929128447485 26.984767005226924 27.589963779520577 23.203340086805017 92-93 22.344928790888698 26.720207063742222 27.55006258513989 23.38480156022919 94-95 22.116385810969444 26.758894451521165 27.564233222606344 23.560486514903044 96-97 22.07898063375689 26.698033663775206 27.59896344329247 23.62402225917543 98-99 22.008952494477644 26.694067690196295 27.316029068194236 23.980950747131825 100-101 22.057771812768976 26.192477943112475 28.02770560397151 23.72204464014704 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 212.0 1 157.0 2 139.5 3 199.0 4 199.0 5 204.0 6 383.5 7 989.0 8 1712.5 9 2058.5 10 2046.5 11 1964.5 12 2078.5 13 2310.0 14 2547.5 15 2741.0 16 2843.0 17 2926.5 18 3094.5 19 3313.5 20 3665.5 21 4131.0 22 4818.5 23 6000.0 24 7093.5 25 8408.0 26 10880.0 27 13772.5 28 16205.0 29 19512.5 30 23533.0 31 27547.5 32 32108.0 33 36781.5 34 41783.5 35 46382.5 36 51382.0 37 57370.5 38 61533.0 39 63479.5 40 66187.5 41 71876.0 42 76766.0 43 79981.0 44 83016.0 45 85942.0 46 87218.0 47 90476.5 48 101617.0 49 101354.0 50 91645.5 51 85703.5 52 80077.0 53 77645.0 54 74796.5 55 68135.5 56 60037.5 57 54029.5 58 48726.5 59 42191.0 60 35364.5 61 28958.0 62 23616.5 63 18365.0 64 14017.0 65 11059.0 66 8337.5 67 6771.0 68 5481.5 69 3748.0 70 2455.5 71 1836.0 72 1525.5 73 1272.5 74 985.5 75 774.0 76 536.0 77 303.0 78 180.0 79 118.5 80 73.0 81 45.5 82 34.5 83 20.0 84 7.5 85 3.0 86 3.5 87 2.5 88 0.5 89 0.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29636749746542346 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0011937822514644836 16-17 9.063902279637745E-4 18-19 8.842831492329507E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.1053539365411885E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.001812780455927549 36-37 0.007958548343096556 38-39 0.0029844556286612086 40-41 0.0032939547308927413 42-43 1.9896370857741392E-4 44-45 0.0016359238260809587 46-47 2.4317786603906145E-4 48-49 4.642486533472991E-4 50-51 0.0 52-53 4.4214157461647535E-5 54-55 0.0 56-57 9.063902279637745E-4 58-59 0.0024980998965830857 60-61 8.400689917713031E-4 62-63 0.0016359238260809587 64-65 2.2107078730823768E-5 66-67 3.7582033842400406E-4 68-69 0.0019233158495816677 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.25469565405782063 78-79 8.842831492329507E-5 80-81 1.3264247238494259E-4 82-83 0.0 84-85 0.0 86-87 0.103991698349795 88-89 0.3658500459164025 90-91 0.26922000478397184 92-93 0.0 94-95 0.0 96-97 0.0 98-99 1.3264247238494259E-4 100-101 4.642486533472991E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2261719.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.955817547652494 #Duplication Level Percentage of deduplicated Percentage of total 1 70.21652122611789 34.375072019766954 2 15.560511454045725 15.235551193848384 3 5.9798645467575104 8.782474731322091 4 2.7970683033635346 5.477310621111485 5 1.4899837195216659 3.6471685560937654 6 0.9557326913146643 2.8073205156196552 7 0.5897884595825794 2.021150335332504 8 0.4174836601629749 1.6350603116851807 9 0.29351059870638574 1.2932146186714861 >10 1.5246214058019014 13.680557606581804 >50 0.11608965494979845 3.9331913946798776 >100 0.054941687376496594 4.819617804187919 >500 0.0028893710130400746 0.9383098181410393 >1k 8.126355974174491E-4 0.7567656327589147 >5k 1.8058568831498867E-4 0.5972348401989309 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7474 0.33045661286835365 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6037 0.26692086859596614 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4396 0.19436543620140256 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3133 0.13852295532734174 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.2107078730823768E-5 0.0 18-19 0.0 0.0 0.0 4.4214157461647535E-5 0.0 20-21 0.0 0.0 0.0 8.842831492329507E-5 0.0 22-23 0.0 0.0 0.0 1.7685662984659014E-4 0.0 24-25 0.0 0.0 0.0 2.87392023500709E-4 0.0 26-27 0.0 0.0 0.0 6.411052831938892E-4 0.0 28-29 0.0 0.0 0.0 0.001414853038772721 0.0 30-31 0.0 0.0 0.0 0.0046203794547421675 0.0 32-33 0.0 0.0 0.0 0.01226942869560719 0.0 34-35 0.0 0.0 0.0 0.023654574241981433 0.0 36-37 0.0 0.0 0.0 0.04302037521018305 0.0 38-39 0.0 0.0 0.0 0.07540724555083987 0.0 40-41 0.0 0.0 0.0 0.11117649893731273 0.0 42-43 0.0 0.0 0.0 0.1520082733531442 0.0 44-45 0.0 0.0 0.0 0.20400412252804173 0.0 46-47 0.0 0.0 0.0 0.2643343403844598 0.0 48-49 0.0 0.0 0.0 0.3314072172537791 0.0 50-51 0.0 0.0 0.0 0.4076987459538519 0.0 52-53 0.0 0.0 0.0 0.48361445431550076 0.0 54-55 0.0 0.0 0.0 0.5725954462070664 0.0 56-57 0.0 0.0 0.0 0.6813401664839885 0.0 58-59 0.0 0.0 0.0 0.8012489615199766 0.0 60-61 0.0 0.0 0.0 0.9365884975100798 0.0 62-63 0.0 0.0 0.0 1.0755978085694995 0.0 64-65 0.0 0.0 0.0 1.2289988278826858 0.0 66-67 0.0 0.0 0.0 1.4072482036893177 0.0 68-69 0.0 0.0 0.0 1.6063887688965783 4.4214157461647535E-5 70-71 0.0 0.0 0.0 1.8344453930837563 4.4214157461647535E-5 72-73 0.0 0.0 0.0 2.073201843376653 4.4214157461647535E-5 74-75 0.0 0.0 0.0 2.3274553558598567 4.4214157461647535E-5 76-77 0.0 0.0 0.0 2.604302302805963 4.4214157461647535E-5 78-79 0.0 0.0 0.0 2.921384133042168 4.4214157461647535E-5 80-81 0.0 0.0 0.0 3.2683547337224477 4.4214157461647535E-5 82-83 0.0 0.0 0.0 3.6332762823321554 4.4214157461647535E-5 84-85 0.0 0.0 0.0 4.018005773484681 4.4214157461647535E-5 86-87 0.0 0.0 0.0 4.423759096510221 4.4214157461647535E-5 88-89 0.0 0.0 0.0 4.876025713185413 4.4214157461647535E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 14505 0.0 26.80489 1 GGTATCA 10475 0.0 24.214912 1 GAGTACT 7555 0.0 22.551105 12-13 TATCAAC 17630 0.0 21.910927 2 ATCAACG 17555 0.0 21.896406 3 GTACATG 11675 0.0 21.766775 1 TCAACGC 17650 0.0 21.724775 4 CAACGCA 17745 0.0 21.635212 5 TACATGG 11615 0.0 21.409214 2 AGTACTT 8065 0.0 21.213326 12-13 AACGCAG 18190 0.0 21.105928 6 GTACTTT 8195 0.0 20.413527 14-15 ACATGGG 11965 0.0 20.18802 3 GTGTAGC 1335 0.0 19.249624 1 ACGCAGA 20050 0.0 19.100637 7 CGCAGAG 20155 0.0 18.977581 8 CATGGGG 5325 0.0 18.804054 4 AGAGTAC 16440 0.0 18.590332 10-11 GCAGAGT 20925 0.0 18.098211 9 TACTTTT 9460 0.0 18.085144 14-15 >>END_MODULE