FastQCFastQC Report
Fri 27 May 2016
SRR939278_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939278_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2307760
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76580.331836932783305No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56490.24478281970395535No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40340.17480153915485147No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29030.12579297673934897No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA138900.028.8974291
GTACATG119100.024.7676371
ATCAACG164000.024.3486613
TACATGG118800.024.3112722
GGTATCA97750.024.2972981
TCAACGC165850.024.0479474
TATCAAC167100.023.9537142
CAACGCA167500.023.8393675
AACGCAG171450.023.3312846
ACATGGG122400.022.7825743
GAGTACT70150.022.7700612-13
ACGCAGA186800.021.3633297
AGTACTT74700.021.25622412-13
CGCAGAG187850.021.1177648
GTACTTT76000.020.67502214-15
CATGGGG52750.020.3181154
GCAGAGT198050.019.527189
TAAGGTG9900.019.1611795
AGAGTAC157400.018.80427710-11
TATACGG1559.732735E-418.3576455
Produced by FastQC (version 0.11.5)