##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939278_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2307760 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.926533521683364 33.0 31.0 34.0 30.0 34.0 2 32.01403568828648 33.0 31.0 34.0 30.0 34.0 3 32.06432124657677 34.0 31.0 34.0 30.0 34.0 4 35.56350053731757 37.0 35.0 37.0 33.0 37.0 5 35.61107177522793 37.0 35.0 37.0 33.0 37.0 6 35.70251542621416 37.0 35.0 37.0 33.0 37.0 7 35.62755615835269 37.0 35.0 37.0 33.0 37.0 8 35.60800993170867 37.0 35.0 37.0 33.0 37.0 9 37.24808472284813 39.0 37.0 39.0 34.0 39.0 10-11 37.159416490449615 39.0 37.0 39.0 33.5 39.0 12-13 37.056057605643566 39.0 37.0 39.0 33.0 39.0 14-15 38.29464914029188 40.0 38.0 41.0 33.0 41.0 16-17 38.23842513952924 40.0 38.0 41.0 33.0 41.0 18-19 38.186235787083575 40.0 38.0 41.0 33.0 41.0 20-21 38.09148589974694 40.0 38.0 41.0 33.0 41.0 22-23 38.01902862516033 40.0 38.0 41.0 33.0 41.0 24-25 38.04930430374043 40.0 38.0 41.0 33.0 41.0 26-27 37.97427462127777 40.0 38.0 41.0 33.0 41.0 28-29 37.843417643082475 40.0 38.0 41.0 33.0 41.0 30-31 37.679629380871496 40.0 37.5 41.0 32.5 41.0 32-33 37.488701164765835 40.0 37.0 41.0 32.0 41.0 34-35 37.19178835754151 40.0 37.0 41.0 31.0 41.0 36-37 36.955330926959476 40.0 36.0 41.0 30.5 41.0 38-39 36.64910995944119 39.5 35.5 41.0 30.0 41.0 40-41 36.53034652650189 39.0 36.0 41.0 30.0 41.0 42-43 36.364444526293894 39.0 35.5 41.0 30.0 41.0 44-45 36.31726457690574 39.0 36.0 41.0 30.0 41.0 46-47 36.11544853017645 39.5 35.5 41.0 29.0 41.0 48-49 35.92303987416369 39.0 35.0 41.0 28.0 41.0 50-51 34.79461664124519 38.0 34.0 40.0 25.5 40.5 52-53 34.91369228169307 38.5 34.5 39.5 26.5 40.5 54-55 35.414807432315314 39.0 35.0 40.5 27.0 41.0 56-57 35.322908578015046 39.0 35.0 41.0 26.5 41.0 58-59 35.12017692481021 39.0 35.0 41.0 26.0 41.0 60-61 34.83013441605713 38.0 34.5 40.0 26.0 41.0 62-63 34.479369388497936 38.0 34.0 40.0 25.5 41.0 64-65 34.10613062016847 37.0 34.0 40.0 24.0 41.0 66-67 33.75101722016154 37.0 34.0 39.5 23.0 41.0 68-69 33.33986246403438 36.0 33.0 39.0 22.0 41.0 70-71 32.922315578743024 36.0 33.0 39.0 22.0 40.5 72-73 32.48441129060214 35.0 32.5 38.5 20.5 40.0 74-75 32.031255633168094 35.0 32.0 37.0 19.5 39.5 76-77 31.583421802960448 35.0 31.5 37.0 18.0 39.0 78-79 31.154369171837626 35.0 31.0 36.0 15.5 39.0 80-81 30.695993300863172 35.0 31.0 36.0 10.0 37.5 82-83 30.256703470031546 34.0 30.5 35.5 7.0 37.0 84-85 29.84477198668839 34.0 30.0 35.0 4.0 36.5 86-87 29.507658725344058 34.0 30.0 35.0 2.0 36.0 88-89 29.10089285714286 34.0 29.0 35.0 2.0 36.0 90-91 28.70217375290325 34.0 29.0 35.0 2.0 35.5 92-93 28.291603329635663 34.0 29.0 35.0 2.0 35.0 94-95 27.818823881166153 34.0 28.0 35.0 2.0 35.0 96-97 27.252128037577563 34.0 27.0 35.0 2.0 35.0 98-99 26.51728212639096 33.0 25.0 35.0 2.0 35.0 100-101 23.976137899954935 31.0 12.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3032062259539323 1101 2 0.19242597770287517 1101 3 0.32852040808838723 1101 4 0.3639139295270084 1101 5 0.2939086480059885 1101 6 0.3297947884678649 1101 7 0.35994856679068477 1101 8 0.32707700869341494 1101 9 0.49068783339983923 1101 10-11 0.43364409025879525 1101 12-13 0.3095943876627061 1101 14-15 0.47903454665417655 1101 16-17 0.4953640406647253 1101 18-19 0.49331125059381264 1101 20-21 0.5713655401207305 1101 22-23 0.5629408181631277 1101 24-25 0.6233673756084528 1101 26-27 0.5036362159106957 1101 28-29 0.4673023656628459 1101 30-31 0.5681520014400405 1101 32-33 0.4918483987431159 1101 34-35 0.41364874251214445 1101 36-37 0.4764819401990863 1101 38-39 0.6377006577413269 1101 40-41 0.471713433927178 1101 42-43 0.3735929206031656 1101 44-45 0.400025993413081 1101 46-47 0.5187790802697805 1101 48-49 0.4135042480596667 1101 50-51 0.43948587679579276 1101 52-53 0.4860688699433169 1101 54-55 0.5249283538344045 1101 56-57 0.6344669280147386 1101 58-59 0.48015347933307595 1101 60-61 0.5243222783044956 1101 62-63 0.6774827027517958 1101 64-65 0.6307965579822152 1101 66-67 0.5479163073601256 1101 68-69 0.47476141911686653 1101 70-71 0.5308395471189229 1101 72-73 0.5493906452228572 1101 74-75 0.41846945517622913 1101 76-77 0.5542440670642499 1101 78-79 0.4821538098402982 1101 80-81 0.4527999599995596 1101 82-83 0.3058773223107991 1101 84-85 0.2735830566260411 1101 86-87 0.3486260561260828 1101 88-89 0.4335619324224069 1101 90-91 0.6183771877437998 1101 92-93 0.5336399899508351 1101 94-95 0.26560961015925955 1101 96-97 0.3682855331639452 1101 98-99 0.5920880930220136 1101 100-101 0.4225798519648585 1102 1 0.47235643765163715 1102 2 0.4129803259362319 1102 3 0.4894099599636874 1102 4 0.4284063233971409 1102 5 0.349582567973016 1102 6 0.44629948141252385 1102 7 0.4289264228362484 1102 8 0.5309988023162546 1102 9 0.5451398079961152 1102 10-11 0.5315099010797439 1102 12-13 0.5901321326138103 1102 14-15 0.6897242268595036 1102 16-17 0.5745757477350608 1102 18-19 0.6347853831952577 1102 20-21 0.6685929793278902 1102 22-23 0.6237052014296438 1102 24-25 0.6097871084571906 1102 26-27 0.6002647426445478 1102 28-29 0.6851753657752511 1102 30-31 0.6060587701462481 1102 32-33 0.6789405762319731 1102 34-35 0.6858062793250639 1102 36-37 0.6936535745802885 1102 38-39 0.7748297184030193 1102 40-41 0.6806681999356456 1102 42-43 0.6518115921532441 1102 44-45 0.6652353259096202 1102 46-47 0.7982305865083106 1102 48-49 0.6416982137384082 1102 50-51 0.5696845307356213 1102 52-53 0.604122890326245 1102 54-55 0.54536116323154 1102 56-57 0.47431094146597985 1102 58-59 0.375176124430304 1102 60-61 0.44346089814788314 1102 62-63 0.48011264860963365 1102 64-65 0.35964470234880963 1102 66-67 0.41587478276197487 1102 68-69 0.5101922145756745 1102 70-71 0.4134625378641701 1102 72-73 0.38741005396803274 1102 74-75 0.300219309985323 1102 76-77 0.34438010157284893 1102 78-79 0.11769092250843727 1102 80-81 0.011527923767406634 1102 82-83 0.09277091611349064 1102 84-85 0.20636528900642048 1102 86-87 0.16058170733069588 1102 88-89 0.08064956684108182 1102 90-91 -0.03256912909084875 1102 92-93 0.288124515410761 1102 94-95 0.21801462720748077 1102 96-97 -0.03848504647560702 1102 98-99 -0.08150636536908351 1102 100-101 -0.22604141990744964 1103 1 0.17632671986111959 1103 2 0.20120912371652366 1103 3 0.2622178621943476 1103 4 0.24350024110734125 1103 5 0.24958188344159993 1103 6 0.25701810249244517 1103 7 0.22466042305529754 1103 8 0.2650526429938509 1103 9 0.328618642284745 1103 10-11 0.3333221980020866 1103 12-13 0.3181549092557674 1103 14-15 0.46881578521809075 1103 16-17 0.44981145471910366 1103 18-19 0.44444195378414264 1103 20-21 0.42920424306415583 1103 22-23 0.3293289692730994 1103 24-25 0.36470866115698897 1103 26-27 0.32920559000317695 1103 28-29 0.35285133598443963 1103 30-31 0.2462213691751316 1103 32-33 0.2705875816352048 1103 34-35 0.25806039564064065 1103 36-37 0.250774298540712 1103 38-39 0.47370297969322195 1103 40-41 0.44383105452292426 1103 42-43 0.17823918242012127 1103 44-45 0.21648105899713244 1103 46-47 0.45471560896093877 1103 48-49 0.3400761024609835 1103 50-51 0.27841629938225054 1103 52-53 0.40519783282560695 1103 54-55 0.36379901690609984 1103 56-57 0.41199473815101584 1103 58-59 0.31275632869447634 1103 60-61 0.34877251966589284 1103 62-63 0.3573311008384721 1103 64-65 0.28528553784221344 1103 66-67 0.2499605682389543 1103 68-69 0.3342261125713648 1103 70-71 0.29553420373982675 1103 72-73 0.4239329994610799 1103 74-75 0.5041739620608254 1103 76-77 0.5530413634836897 1103 78-79 0.49156737827858166 1103 80-81 0.46884567868690397 1103 82-83 0.34810672440818635 1103 84-85 0.27447251400733563 1103 86-87 0.1726580964731177 1103 88-89 0.24746063160688436 1103 90-91 0.27692646252683417 1103 92-93 0.3025795371332265 1103 94-95 0.19892943499106153 1103 96-97 0.026963458507783145 1103 98-99 -0.11633760555746875 1103 100-101 -0.2925433046506143 1104 1 0.19738103408788632 1104 2 0.3144442693826477 1104 3 0.36765782827254867 1104 4 0.33191860339568535 1104 5 0.31232192773689604 1104 6 0.33046370361855537 1104 7 0.34757052151351076 1104 8 0.3765730380047714 1104 9 0.3857364537709884 1104 10-11 0.3271127187381566 1104 12-13 0.35858071239735523 1104 14-15 0.480062723926153 1104 16-17 0.5021731769519775 1104 18-19 0.4334386144382023 1104 20-21 0.5212053258279141 1104 22-23 0.6315078514679087 1104 24-25 0.658933748766195 1104 26-27 0.6333494545929526 1104 28-29 0.6735535496472735 1104 30-31 0.5941985052335212 1104 32-33 0.7517620835380185 1104 34-35 0.5467712795072543 1104 36-37 0.6280528293993726 1104 38-39 0.7232095420374591 1104 40-41 0.6206827300526143 1104 42-43 0.6313661461378643 1104 44-45 0.7707534680587997 1104 46-47 0.8218306393450945 1104 48-49 0.8425324428678849 1104 50-51 0.7859631582544822 1104 52-53 0.7585400003360832 1104 54-55 0.7274041705558076 1104 56-57 0.7600398841836054 1104 58-59 0.7807575796283501 1104 60-61 0.83691562704049 1104 62-63 0.7840545691358187 1104 64-65 0.7372625147235468 1104 66-67 0.8296330726719745 1104 68-69 0.7792576410961018 1104 70-71 0.4382493687500073 1104 72-73 0.43012404296340634 1104 74-75 0.5788253069608729 1104 76-77 0.5187862570587676 1104 78-79 0.47505163380093407 1104 80-81 0.313502129393612 1104 82-83 0.3607897414970118 1104 84-85 0.1831107250010291 1104 86-87 0.33509604158715334 1104 88-89 0.287390242969785 1104 90-91 0.3663347352825781 1104 92-93 0.41971317189875634 1104 94-95 0.4547448498507798 1104 96-97 0.42418667308858105 1104 98-99 0.35788391630565997 1104 100-101 0.1404567535794108 1105 1 0.29999822232394635 1105 2 0.3648500334719884 1105 3 0.3720822541357549 1105 4 0.35402451880025865 1105 5 0.32872536666369 1105 6 0.2249183706176794 1105 7 0.32797644774196044 1105 8 0.30564878808441875 1105 9 0.4459479094691048 1105 10-11 0.4244471297853494 1105 12-13 0.426924321114484 1105 14-15 0.39097418238501547 1105 16-17 0.44552812445176926 1105 18-19 0.3609495029561671 1105 20-21 0.5268321008677788 1105 22-23 0.5226660549840503 1105 24-25 0.4179005895644323 1105 26-27 0.39489978498426836 1105 28-29 0.4244436714750961 1105 30-31 0.4731391978129409 1105 32-33 0.4539554751859285 1105 34-35 0.47478994829254617 1105 36-37 0.580957442081889 1105 38-39 0.6564573068183037 1105 40-41 0.6807486987615192 1105 42-43 0.3513781882537188 1105 44-45 0.4887171892128279 1105 46-47 0.6031187776418747 1105 48-49 0.655720575511701 1105 50-51 0.6390462773377621 1105 52-53 0.5863139705226459 1105 54-55 0.6803318990174958 1105 56-57 0.6129408158700116 1105 58-59 0.7012028180188565 1105 60-61 0.8907886484503038 1105 62-63 0.7906368694165806 1105 64-65 0.7021202858541002 1105 66-67 0.7445114617629045 1105 68-69 0.6844672803979179 1105 70-71 0.5539111965135746 1105 72-73 0.8617002411166794 1105 74-75 0.8345849703425401 1105 76-77 0.7659589014732227 1105 78-79 0.687501639081411 1105 80-81 0.7774531265710785 1105 82-83 0.7350241271716236 1105 84-85 0.6489451651894456 1105 86-87 0.6287008366980906 1105 88-89 0.6672140459558626 1105 90-91 0.8231507349951102 1105 92-93 0.7054242023103541 1105 94-95 0.7967495948300538 1105 96-97 0.7228007999628758 1105 98-99 0.8520354050713905 1105 100-101 0.6404437167181811 1106 1 0.07460951002305549 1106 2 0.2509980774155167 1106 3 0.16487366046437302 1106 4 0.2824857498878117 1106 5 0.22318008731758 1106 6 0.11954993657866453 1106 7 0.20892187117489414 1106 8 0.08682777364078476 1106 9 0.07893873835569565 1106 10-11 0.0818726115940791 1106 12-13 0.2758811653857691 1106 14-15 0.3143785850752536 1106 16-17 0.368701259796957 1106 18-19 0.37986247695948805 1106 20-21 0.36574107900927544 1106 22-23 0.38917942764722113 1106 24-25 0.44707374933111055 1106 26-27 0.32958028656808835 1106 28-29 0.3667582060119301 1106 30-31 0.2536378552623191 1106 32-33 0.2842816663548646 1106 34-35 0.34877282461409465 1106 36-37 0.25852897285066234 1106 38-39 0.46423003701931265 1106 40-41 0.5358940761304538 1106 42-43 0.47177489438355735 1106 44-45 0.4332783960052069 1106 46-47 0.26481854736402255 1106 48-49 0.36302559198146866 1106 50-51 0.42234298544658344 1106 52-53 0.3180879563253356 1106 54-55 0.5694593653253079 1106 56-57 0.5118009847067597 1106 58-59 0.6395152800971218 1106 60-61 0.5225673501278507 1106 62-63 0.7214858279450951 1106 64-65 0.7280604397179431 1106 66-67 0.6423114008775883 1106 68-69 0.644255710251592 1106 70-71 0.6759909857476529 1106 72-73 0.6467637180554178 1106 74-75 0.6774794943276063 1106 76-77 0.6689804088099898 1106 78-79 0.6547642042562742 1106 80-81 0.660957543703681 1106 82-83 0.5215985788353841 1106 84-85 0.46338622664866236 1106 86-87 0.6293701119733903 1106 88-89 0.6835356046198733 1106 90-91 0.5699029919833407 1106 92-93 0.635558516321094 1106 94-95 0.6574377041305581 1106 96-97 0.7302450400059577 1106 98-99 0.6926458877032893 1106 100-101 0.47141676170383917 1107 1 0.11015331425349117 1107 2 0.1445312605635749 1107 3 0.14672059967936946 1107 4 0.15395864202476162 1107 5 0.2211467018622315 1107 6 0.11333614033334527 1107 7 0.16809704785760715 1107 8 0.11805306025289752 1107 9 0.2871815928438721 1107 10-11 0.3007164289476947 1107 12-13 0.33268149101861866 1107 14-15 0.41893677769161997 1107 16-17 0.36197557857946805 1107 18-19 0.3412834567010563 1107 20-21 0.3930933082415393 1107 22-23 0.3974703869882035 1107 24-25 0.29204307161886334 1107 26-27 0.344406448894766 1107 28-29 0.3111300696675343 1107 30-31 0.3669989932949349 1107 32-33 0.4313327822600641 1107 34-35 0.5374350426097578 1107 36-37 0.4467579640823942 1107 38-39 0.5083545620622445 1107 40-41 0.522915876182104 1107 42-43 0.5451146753982954 1107 44-45 0.5454397436043195 1107 46-47 0.5636777809176294 1107 48-49 0.4312231684593968 1107 50-51 0.48678195324868767 1107 52-53 0.5440656080927084 1107 54-55 0.508862214046566 1107 56-57 0.5292932356600133 1107 58-59 0.5391992722620387 1107 60-61 0.4844560327888914 1107 62-63 0.39210528863286953 1107 64-65 0.5915682277904892 1107 66-67 0.3613740053297221 1107 68-69 0.5070897472961775 1107 70-71 0.4278228221822573 1107 72-73 0.36506784708561923 1107 74-75 0.28969311222706295 1107 76-77 0.38744781933930383 1107 78-79 0.3580131724486364 1107 80-81 0.3309121504358927 1107 82-83 0.3902473582076027 1107 84-85 0.392584234432384 1107 86-87 0.4343791435129063 1107 88-89 0.44492431225337725 1107 90-91 0.5106763942662909 1107 92-93 0.5798730545215847 1107 94-95 0.6813586965100136 1107 96-97 0.6175345003924484 1107 98-99 0.7623557642826952 1107 100-101 0.7719935346426645 1108 1 0.07727693614755182 1108 2 0.1402579365937271 1108 3 0.11830177345746051 1108 4 0.2245118240417483 1108 5 0.24289208666661466 1108 6 0.09818764213448361 1108 7 0.06543265403522014 1108 8 0.1606587722346049 1108 9 0.25241043541338826 1108 10-11 0.18250501281158193 1108 12-13 0.18413594653731735 1108 14-15 0.1965019986687011 1108 16-17 0.2418001783323902 1108 18-19 0.13673128994214068 1108 20-21 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5 1916.0 6 2285.0 7 2751.0 8 3364.0 9 4147.0 10 5278.0 11 6565.0 12 8600.0 13 12130.0 14 11298.0 15 12381.0 16 15657.0 17 20337.0 18 17078.0 19 12472.0 20 12681.0 21 13496.0 22 14805.0 23 16355.0 24 18488.0 25 21179.0 26 24798.0 27 28714.0 28 34111.0 29 40998.0 30 49774.0 31 60238.0 32 75678.0 33 98574.0 34 134124.0 35 192001.0 36 300565.0 37 482307.0 38 470034.0 39 72866.0 40 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.3120697994487 19.44459775229922 15.606358157182608 22.63697429106947 2 14.893341968990587 20.391936216445547 40.886814904404055 23.82790691015981 3 18.99856871450258 25.04727927768457 29.8058371631826 26.14831484463025 4 12.826403296637503 17.045729651775744 37.66585361409245 32.462013437494306 5 13.943430176328912 37.5677900245886 33.662425094165165 14.82635470491733 6 30.991022630947967 36.138278799230825 18.124139605568093 14.746558964253117 7 28.08937119198736 32.92932788183979 20.926545764230276 18.054755161942573 8 25.96631392892717 34.6001855114596 20.28805012191806 19.145450437695168 9 26.757798525568393 17.261152876988902 20.621575129362924 35.359473468079784 10-11 25.08550796151968 26.855657184186406 27.017393845681543 21.04144100861237 12-13 26.003933866637063 24.22252797763177 27.53635097444721 22.23718718128395 14-15 22.974274097856814 25.394862104230025 26.351775733110923 25.27908806480224 16-17 22.119889801858854 28.640958247722075 26.851116518252137 22.388035432166937 18-19 22.068191259536654 27.188237987738617 29.240033615165327 21.503537137559398 20-21 23.21346952262917 26.288418156761082 28.86167685600124 21.636435464608507 22-23 22.68157035130114 26.302330442686966 28.851696657339577 22.16440254867232 24-25 22.433619711355067 26.477493949065234 28.994262102574325 22.094624237005373 26-27 22.2102033071293 26.671921930062346 28.96193006234752 22.155944700460832 28-29 21.95898319970075 26.91179165243602 29.12646033578914 22.002764812074098 30-31 22.23188601895087 26.498173997780434 28.96591158948712 22.304028393781575 32-33 22.080549311019524 26.61386564533811 28.77796598115538 22.527619062486988 34-35 22.655191039524063 26.78577827856471 28.518741900250394 22.04028878166083 36-37 22.697780517745095 26.37270731603892 28.70689852009146 22.222613646124522 38-39 22.358435252154376 26.630905854814785 28.741053184460874 22.269605708569966 40-41 22.50964874985763 26.52017260372352 28.743819279067324 22.226359367351535 42-43 22.29919908790205 26.59398639766966 28.45636031347714 22.650454200951145 44-45 22.44133542019012 26.7742450328793 28.277482081748367 22.506937465182208 46-47 22.675774535239714 26.978048610236762 27.855282222368643 22.49089463215488 48-49 22.61599005889591 26.876233341060857 27.94247938686666 22.565297213176574 50-51 22.5377408568084 27.118660285208474 27.866986217990046 22.47661263999308 52-53 22.70232678603776 27.148265441104673 27.589627810352486 22.559779962505075 54-55 22.32156757353817 26.98745060998785 27.835941230342925 22.85504058613105 56-57 22.520354347046805 27.193729385062092 27.696411595950803 22.589504671940293 58-59 22.72993925204089 26.802415138367934 27.795022314534414 22.672623295056766 60-61 22.555988644434226 26.968730046342998 28.0482127063367 22.42706860288608 62-63 22.51292714361 26.904329571286695 28.05131462919578 22.531428655907526 64-65 22.24145186457818 26.910753606011017 27.95045914456122 22.897335384849587 66-67 22.245118595845028 27.034472079340393 27.74943339630984 22.97097592850474 68-69 22.324263186514408 26.91042958129418 27.910647090810226 22.854660141381185 70-71 22.35292966116073 26.94883696649889 27.762916914992193 22.935316457348186 72-73 22.49206211126093 26.85361663259536 27.743921534513504 22.910399721630203 74-75 22.291384661959725 27.235021417858775 27.528168572989486 22.945425347192018 76-77 22.379367901372017 26.922402830666247 27.590254450314607 23.107974817647133 78-79 22.433345587667805 27.0154593755768 27.628255813978768 22.922939222776627 80-81 22.366580873249305 27.208825409924632 27.559209523699792 22.86538419312628 82-83 22.45901903110275 27.079597182819303 27.533544905992507 22.927838880085442 84-85 22.475805537996944 27.042718851512422 27.47397574584959 23.007499864641044 86-87 22.45744912158636 26.98991128891981 27.515850582710037 23.03678900678379 88-89 22.459249149667077 26.84233726135932 27.540065843309968 23.158347745663637 90-91 22.34875698734442 27.013570005257296 27.715181061373784 22.922491946024497 92-93 22.57853117027827 26.73871533460805 27.640461950747262 23.042291544366417 94-95 22.37797768126981 26.812230474482362 27.61561689058115 23.19417495366668 96-97 22.364542477404896 26.72362715379984 27.695468972405607 23.216361396389658 98-99 22.365470632577615 26.686361087779293 27.379000669433427 23.569167610209664 100-101 22.518942383754887 26.319813278955657 27.859361688526025 23.301882648763435 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1650.0 1 928.5 2 237.0 3 285.5 4 279.0 5 305.0 6 485.0 7 1139.0 8 1957.0 9 2404.5 10 2474.0 11 2393.0 12 2550.5 13 2758.0 14 2951.5 15 3228.0 16 3358.0 17 3461.5 18 3593.5 19 3713.0 20 3989.0 21 4416.5 22 5069.5 23 6125.0 24 7235.0 25 8720.0 26 11151.5 27 13874.5 28 16402.5 29 19890.5 30 24055.0 31 28184.0 32 32812.0 33 37337.0 34 42536.5 35 47198.0 36 52178.0 37 58427.0 38 62755.5 39 64646.5 40 67147.0 41 72974.0 42 77818.0 43 80777.0 44 83911.5 45 87256.0 46 88845.5 47 91825.0 48 102617.5 49 102664.5 50 92916.0 51 87033.5 52 81260.0 53 78596.0 54 75857.0 55 69241.5 56 61074.0 57 54850.0 58 49836.0 59 43033.0 60 35957.0 61 29780.0 62 24171.5 63 18824.0 64 14563.5 65 11500.5 66 8748.0 67 7147.5 68 5796.5 69 4000.0 70 2679.5 71 2027.5 72 1680.0 73 1406.5 74 1056.5 75 839.5 76 605.5 77 355.0 78 214.0 79 138.5 80 102.0 81 72.5 82 53.5 83 46.0 84 27.5 85 13.5 86 9.5 87 9.5 88 11.0 89 11.0 90 8.5 91 7.0 92 6.5 93 5.5 94 4.5 95 5.5 96 3.5 97 3.0 98 2.5 99 2.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28828821021250045 2 0.11218670918986377 3 0.12301972475474053 4 0.12418969043574721 5 0.1314261448330849 6 0.06088154747460741 7 0.0694612958019898 8 0.07379450202794052 9 0.07154123479044615 10-11 0.06681804000415988 12-13 0.07071792560751551 14-15 0.06688303809754914 16-17 0.06718636253336568 18-19 0.07184455922626269 20-21 0.07600443720317537 22-23 0.08371754428536762 24-25 0.09205896627032273 26-27 0.09251395292404756 28-29 0.08655579436336534 30-31 0.08246091447984193 32-33 0.0755711165805803 34-35 0.24289787499566678 36-37 0.36669757687107846 38-39 0.3401350227060006 40-41 0.3233438485804417 42-43 0.346959822511873 44-45 0.359179464069054 46-47 0.3652676188165147 48-49 0.37347904461469134 50-51 0.36808420286338267 52-53 0.3689941761708323 54-55 0.3555829029015149 56-57 0.3335918813048151 58-59 0.3186423198252851 60-61 0.27444361632058795 62-63 0.34440323083856206 64-65 0.37471400838908725 66-67 0.37935053905085453 68-69 0.39042188095815855 70-71 0.39128852220334864 72-73 0.37525565916733106 74-75 0.3788955523971297 76-77 0.362776025236593 78-79 0.34204163344541894 80-81 0.34039501507955766 82-83 0.35341629978853956 84-85 0.36045775990570944 86-87 0.3535896280375776 88-89 0.3689075120463133 90-91 0.3921768294796686 92-93 0.38754029881790136 94-95 0.38689031788400874 96-97 0.39137518632786766 98-99 0.38136547994592157 100-101 0.35909279994453497 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2307760.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.0257345706011 #Duplication Level Percentage of deduplicated Percentage of total 1 71.46640193040143 35.751592536861565 2 15.004685858912644 15.012408641864317 3 5.638140969786239 8.461564308784736 4 2.6542573107044696 5.3112468682951715 5 1.4593109957418833 3.650155226447153 6 0.9071258506589434 2.7227782216317022 7 0.5633798461167787 1.972844345098485 8 0.3909121699108146 1.5644534761900906 9 0.27802842379826515 1.2517718518813146 >10 1.4679946220686386 13.480462321480688 >50 0.11489331752994464 3.961906611736437 >100 0.051132781449911624 4.627712836428039 >500 0.002693339779586926 0.8811319447073369 >1k 8.688192837375418E-4 0.7716043710544482 >5k 1.7376385674750834E-4 0.578366437538531 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7658 0.331836932783305 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5649 0.24478281970395535 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4034 0.17480153915485147 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2903 0.12579297673934897 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.166603112975353E-5 0.0 14-15 0.0 0.0 0.0 4.333206225950706E-5 0.0 16-17 0.0 0.0 0.0 8.666412451901411E-5 0.0 18-19 0.0 0.0 0.0 8.666412451901411E-5 0.0 20-21 0.0 0.0 0.0 1.2999618677852115E-4 0.0 22-23 0.0 0.0 0.0 1.2999618677852115E-4 0.0 24-25 0.0 0.0 0.0 2.1666031129753525E-4 0.0 26-27 0.0 0.0 0.0 4.983187159843312E-4 0.0 28-29 0.0 0.0 0.0 0.0011916317121364439 0.0 30-31 0.0 0.0 0.0 0.003618227198668839 0.0 32-33 0.0 0.0 0.0 0.011634658716677643 0.0 34-35 0.0 0.0 0.0 0.0233559815578743 0.0 36-37 0.0 0.0 0.0 0.04146878358234825 0.0 38-39 0.0 0.0 0.0 0.07203955350643049 0.0 40-41 0.0 0.0 0.0 0.10971678164107186 0.0 42-43 0.0 0.0 0.0 0.1502539258848407 0.0 44-45 0.0 0.0 0.0 0.19698755503171908 0.0 46-47 0.0 0.0 0.0 0.25854074947134886 0.0 48-49 0.0 0.0 0.0 0.32529379138211945 0.0 50-51 0.0 0.0 0.0 0.3983733143827781 0.0 52-53 0.0 0.0 0.0 0.47576437757825774 0.0 54-55 0.0 0.0 0.0 0.5658734010469026 0.0 56-57 0.0 0.0 0.0 0.6723186119873816 0.0 58-59 0.0 0.0 0.0 0.7895101743682185 0.0 60-61 0.0 0.0 0.0 0.9221929490068291 0.0 62-63 0.0 0.0 0.0 1.060638887925954 0.0 64-65 0.0 0.0 0.0 1.2152910181301348 0.0 66-67 0.0 0.0 0.0 1.3940357749506016 0.0 68-69 0.0 0.0 0.0 1.5903083509550386 0.0 70-71 0.0 0.0 0.0 1.8139667903074843 0.0 72-73 0.0 0.0 0.0 2.0476999341352653 0.0 74-75 0.0 0.0 0.0 2.299827538392207 0.0 76-77 0.0 0.0 0.0 2.5781060422227613 0.0 78-79 0.0 0.0 0.0 2.8931951329427674 0.0 80-81 4.333206225950706E-5 0.0 0.0 3.2422348944430963 0.0 82-83 4.333206225950706E-5 0.0 0.0 3.6051192498353384 0.0 84-85 4.333206225950706E-5 0.0 0.0 3.983494817485354 0.0 86-87 4.333206225950706E-5 0.0 0.0 4.379333206225951 0.0 88-89 4.333206225950706E-5 0.0 0.0 4.822771865358616 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 13890 0.0 28.897429 1 GTACATG 11910 0.0 24.767637 1 ATCAACG 16400 0.0 24.348661 3 TACATGG 11880 0.0 24.311272 2 GGTATCA 9775 0.0 24.297298 1 TCAACGC 16585 0.0 24.047947 4 TATCAAC 16710 0.0 23.953714 2 CAACGCA 16750 0.0 23.839367 5 AACGCAG 17145 0.0 23.331284 6 ACATGGG 12240 0.0 22.782574 3 GAGTACT 7015 0.0 22.77006 12-13 ACGCAGA 18680 0.0 21.363329 7 AGTACTT 7470 0.0 21.256224 12-13 CGCAGAG 18785 0.0 21.117764 8 GTACTTT 7600 0.0 20.675022 14-15 CATGGGG 5275 0.0 20.318115 4 GCAGAGT 19805 0.0 19.52718 9 TAAGGTG 990 0.0 19.161179 5 AGAGTAC 15740 0.0 18.804277 10-11 TATACGG 155 9.732735E-4 18.357645 5 >>END_MODULE