FastQCFastQC Report
Fri 27 May 2016
SRR939278_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939278_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2307760
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76910.33326689083786876No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62070.2689621104447603No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42590.18455125316324056No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT32250.13974590078691024No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGGT909.0004096E-426.363664
GTATCAA147500.025.7123131
GTACATG118600.023.0705531
GGTATCA108850.022.6889171
TACATGG117900.022.5801732
ATCAACG176800.021.4189823
TCAACGC177900.021.3398934
GAGTACT76300.021.33418512-13
TATCAAC178450.021.3007162
AGTACTT81100.021.24184212-13
CAACGCA179450.021.102685
ACATGGG120450.020.6838193
AACGCAG184500.020.5765156
CATGGGG54100.019.9116294
GTACTTT80500.019.80841814-15
ACGCAGA203000.018.5610547
CGCAGAG203050.018.5331138
GTCCCGA3851.2732926E-1118.48886
AGAGTAC164400.018.37397210-11
TACTTTT93400.018.19041414-15
Produced by FastQC (version 0.11.5)