##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939278_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2307760 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.26992191562381 33.0 31.0 34.0 31.0 34.0 2 32.47726236697057 34.0 31.0 34.0 31.0 34.0 3 32.5634281727736 34.0 31.0 34.0 31.0 34.0 4 36.101097601137035 37.0 35.0 37.0 35.0 37.0 5 36.037704527333865 37.0 35.0 37.0 35.0 37.0 6 36.078213072416546 37.0 35.0 37.0 35.0 37.0 7 36.02680781363747 37.0 35.0 37.0 35.0 37.0 8 36.0061943183 37.0 35.0 37.0 35.0 37.0 9 37.71261136340001 39.0 37.0 39.0 35.0 39.0 10-11 37.62876165632475 39.0 37.0 39.0 35.0 39.0 12-13 37.548047240614274 39.0 37.0 39.0 35.0 39.0 14-15 38.831401228897285 40.0 38.0 41.0 35.0 41.0 16-17 38.77514797899262 40.0 38.0 41.0 35.0 41.0 18-19 38.90466772974659 40.0 38.0 41.0 35.0 41.0 20-21 38.91461980448574 40.0 38.0 41.0 35.0 41.0 22-23 38.8610847748466 40.0 38.0 41.0 35.0 41.0 24-25 38.79685777550525 40.0 38.0 41.0 34.0 41.0 26-27 38.631044606024886 40.0 38.0 41.0 34.0 41.0 28-29 38.4829434169931 40.0 38.0 41.0 34.0 41.0 30-31 38.29886448330849 40.0 38.0 41.0 34.0 41.0 32-33 38.108479651263565 40.0 37.5 41.0 33.5 41.0 34-35 38.06581901930876 40.0 38.0 41.0 33.0 41.0 36-37 37.98886755988491 40.0 37.5 41.0 33.0 41.0 38-39 37.8838198079523 40.0 37.0 41.0 33.0 41.0 40-41 37.72001204631331 40.0 37.0 41.0 33.0 41.0 42-43 37.5472575137796 40.0 37.0 41.0 33.0 41.0 44-45 37.31919588692065 40.0 37.0 41.0 32.0 41.0 46-47 37.021973905432105 40.0 36.5 41.0 31.0 41.0 48-49 36.71524226956009 40.0 36.0 41.0 30.5 41.0 50-51 36.47775266925504 39.0 36.0 41.0 30.0 41.0 52-53 36.24764251915277 39.0 35.0 41.0 30.0 41.0 54-55 35.9738436839186 39.0 35.0 40.0 29.0 41.0 56-57 35.73175590182688 38.5 35.0 40.0 28.5 41.0 58-59 35.44234669116373 38.0 35.0 40.0 28.0 41.0 60-61 35.10559070267272 38.0 34.0 40.0 28.0 41.0 62-63 34.72637080978958 37.5 34.0 40.0 27.0 41.0 64-65 34.30415294484695 37.0 34.0 39.5 26.0 41.0 66-67 33.927712587097446 36.0 33.0 39.0 26.0 40.5 68-69 33.54108897805664 36.0 33.0 39.0 26.0 40.0 70-71 33.13007028460498 35.5 33.0 38.5 25.0 40.0 72-73 32.63008328422366 35.0 32.0 37.5 23.5 40.0 74-75 32.11833877872916 35.0 31.5 37.0 22.0 39.0 76-77 30.110609638783927 33.5 29.5 35.0 20.0 37.5 78-79 31.105241229590597 34.0 31.0 36.0 20.0 38.0 80-81 31.14942238361008 34.0 31.0 36.0 20.0 37.0 82-83 30.82947641869172 34.0 31.0 35.0 19.5 37.0 84-85 30.453561245536797 34.0 31.0 35.0 18.0 36.5 86-87 29.91674004229209 34.0 30.0 35.0 11.0 36.0 88-89 29.415998847367145 34.0 29.5 35.0 7.0 36.0 90-91 28.98822949526814 34.0 29.0 35.0 2.0 35.0 92-93 28.631811583526883 34.0 29.0 35.0 2.0 35.0 94-95 28.222252747252746 34.0 29.0 35.0 2.0 35.0 96-97 27.65208513883593 33.0 27.5 35.0 2.0 35.0 98-99 26.88147900994904 33.0 26.5 35.0 2.0 35.0 100-101 23.439531623739036 29.0 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1924547890405961 1101 2 0.17848584630074527 1101 3 0.1732927922875902 1101 4 0.1391856764730406 1101 5 0.1263968237477826 1101 6 0.12568610388956358 1101 7 0.12701350164557113 1101 8 0.13916378766892024 1101 9 0.23932228244402154 1101 10-11 0.21533765423062334 1101 12-13 0.2629282639480195 1101 14-15 0.32722116002695145 1101 16-17 0.28035278971501754 1101 18-19 0.28614360277659046 1101 20-21 0.25885721965982356 1101 22-23 0.3241459459007885 1101 24-25 0.2718022837518177 1101 26-27 0.2748929329686547 1101 28-29 0.22532129109141152 1101 30-31 0.3166445368390143 1101 32-33 0.20549337654937716 1101 34-35 0.3390998893581809 1101 36-37 0.24411187017892644 1101 38-39 0.36423413566501495 1101 40-41 0.4253301721921048 1101 42-43 0.4730017267467943 1101 44-45 0.503284309821268 1101 46-47 0.459694582003209 1101 48-49 0.46696517730387654 1101 50-51 0.5333012695068717 1101 52-53 0.5109017772131779 1101 54-55 0.5308838058130974 1101 56-57 0.5708387433300217 1101 58-59 0.5259465358517232 1101 60-61 0.46154099091999967 1101 62-63 0.5470382076025118 1101 64-65 0.48010752923377 1101 66-67 0.5640798543255627 1101 68-69 0.5215437317052789 1101 70-71 0.4396379521315197 1101 72-73 0.3967669287709654 1101 74-75 0.36856901367663397 1101 76-77 0.5771461320438185 1101 78-79 0.7549686064626222 1101 80-81 0.766320277442059 1101 82-83 0.644116747510953 1101 84-85 0.5272769679212139 1101 86-87 0.5454380830480794 1101 88-89 0.7884493195672704 1101 90-91 0.8681274324756068 1101 92-93 0.7410743075079864 1101 94-95 1.053091761571249 1101 96-97 0.9369527706016605 1101 98-99 0.8642432000444913 1101 100-101 0.8673834775941174 1102 1 0.09438723311868102 1102 2 0.20002351163103071 1102 3 0.21201216169071557 1102 4 0.11240334105395533 1102 5 0.17117908422962813 1102 6 0.16716011940157216 1102 7 0.18251811028583376 1102 8 0.20581460823169806 1102 9 0.27000275941955465 1102 10-11 0.27936766191169227 1102 12-13 0.25066127192882703 1102 14-15 0.3091302564711782 1102 16-17 0.39918241053258186 1102 18-19 0.2956844014202247 1102 20-21 0.3224433412829697 1102 22-23 0.2666700194891618 1102 24-25 0.26028969897906507 1102 26-27 0.3818920711903928 1102 28-29 0.3607826468934192 1102 30-31 0.32984765891177403 1102 32-33 0.37288865175639074 1102 34-35 0.433931210314384 1102 36-37 0.3875156367119601 1102 38-39 0.30815672549602624 1102 40-41 0.39181343776968447 1102 42-43 0.30964498333189994 1102 44-45 0.40017745868409094 1102 46-47 0.5095144984481834 1102 48-49 0.37445328101701847 1102 50-51 0.4787943799643699 1102 52-53 0.4517500667706713 1102 54-55 0.5495423589623343 1102 56-57 0.547200961847004 1102 58-59 0.44149882331097245 1102 60-61 0.2597071361483714 1102 62-63 0.291277290183352 1102 64-65 0.41076177817528503 1102 66-67 0.397594715316977 1102 68-69 0.4497642766863592 1102 70-71 0.4414067051758508 1102 72-73 0.4749018813731638 1102 74-75 0.5551676591860115 1102 76-77 0.5007621348914313 1102 78-79 0.5889065115920786 1102 80-81 0.6566382689366783 1102 82-83 0.5862981003154779 1102 84-85 0.5902295185975213 1102 86-87 0.7490850677608805 1102 88-89 0.7822136044786561 1102 90-91 0.9443951598164553 1102 92-93 0.7654854647263143 1102 94-95 0.8757500899087631 1102 96-97 0.658645228168453 1102 98-99 0.6957924806469826 1102 100-101 0.5081628014728636 1103 1 0.12650270900491734 1103 2 0.14744488178911297 1103 3 0.15007142385712058 1103 4 0.08773123160194274 1103 5 0.1493176607693627 1103 6 0.0870206575345378 1103 7 0.12981329848633294 1103 8 0.09752516096799724 1103 9 0.0891707799372412 1103 10-11 0.13079319858432115 1103 12-13 0.16633088055940703 1103 14-15 0.170477847833304 1103 16-17 0.19152090675397204 1103 18-19 0.17137840218690314 1103 20-21 0.1746228893096955 1103 22-23 0.168901591903456 1103 24-25 0.2540555572901866 1103 26-27 0.21087604402818982 1103 28-29 0.20017617487706474 1103 30-31 0.2957261637126223 1103 32-33 0.25739861853379864 1103 34-35 0.3519135083319753 1103 36-37 0.4467979967937339 1103 38-39 0.40800156504186447 1103 40-41 0.519098745900088 1103 42-43 0.4829381042475305 1103 44-45 0.630989814932498 1103 46-47 0.4890020582305965 1103 48-49 0.4936146843976914 1103 50-51 0.6494623229018259 1103 52-53 0.5289031462493341 1103 54-55 0.48680197434695316 1103 56-57 0.6048524258315311 1103 58-59 0.39338721730900517 1103 60-61 0.2937057579584561 1103 62-63 0.3823654897744504 1103 64-65 0.5705307260048613 1103 66-67 0.46223923815295365 1103 68-69 0.4593213620428571 1103 70-71 0.38190563730684346 1103 72-73 0.5201847753894455 1103 74-75 0.500628987724582 1103 76-77 0.43725176652225883 1103 78-79 0.6762698334861312 1103 80-81 0.5799049213042444 1103 82-83 0.5617600449323241 1103 84-85 0.5068130907562605 1103 86-87 0.41951091475441515 1103 88-89 0.7018614815185593 1103 90-91 0.6544462486777611 1103 92-93 0.5007090555043092 1103 94-95 0.5190427088346894 1103 96-97 0.171048937416014 1103 98-99 0.03687965344096966 1103 100-101 0.23764545544679905 1104 1 0.24509141997871353 1104 2 0.19880803773968836 1104 3 0.22163050698824094 1104 4 0.1522561723770579 1104 5 0.14135431106343077 1104 6 0.11179809629913962 1104 7 0.15743748863368268 1104 8 0.19772397491901472 1104 9 0.2614689347648138 1104 10-11 0.2553236206261147 1104 12-13 0.2357533263689433 1104 14-15 0.333133986930676 1104 16-17 0.2784510309458099 1104 18-19 0.22117530529370555 1104 20-21 0.24310252577445368 1104 22-23 0.20196590550744986 1104 24-25 0.20394119936167954 1104 26-27 0.18556149508719244 1104 28-29 0.2355509165796832 1104 30-31 0.12328917860714483 1104 32-33 0.2540409492457272 1104 34-35 0.2513487894784703 1104 36-37 0.25405503200401114 1104 38-39 0.3581166424147355 1104 40-41 0.20688957284966847 1104 42-43 0.2871523394883013 1104 44-45 0.2925309276925887 1104 46-47 0.1407089287218426 1104 48-49 0.15089353900631153 1104 50-51 -0.029442579369522548 1104 52-53 0.10338800722907848 1104 54-55 0.1379151908320253 1104 56-57 0.11338200072760429 1104 58-59 0.22996127275163047 1104 60-61 0.2533241331968057 1104 62-63 0.20823095323039098 1104 64-65 0.18651538565260495 1104 66-67 0.3740300767934954 1104 68-69 0.27307497978004136 1104 70-71 0.08930166017347574 1104 72-73 0.2007689057509694 1104 74-75 -0.012200840977961036 1104 76-77 0.11782431832618911 1104 78-79 0.2829484804001545 1104 80-81 0.3100675359628795 1104 82-83 0.28758497219537915 1104 84-85 0.19886662551003198 1104 86-87 0.2328421961724949 1104 88-89 0.43791724475121896 1104 90-91 0.574268784237983 1104 92-93 0.5579324229670348 1104 94-95 0.6035154809010024 1104 96-97 0.43253110070425294 1104 98-99 0.5366751738656568 1104 100-101 0.49057623568195297 1105 1 0.056745804242282816 1105 2 0.05522925061914208 1105 3 0.08697791633385066 1105 4 0.11371044629838423 1105 5 0.20799195336788756 1105 6 0.09509009876541086 1105 7 0.11435383931166143 1105 8 0.12777299789938468 1105 9 0.14787536669201984 1105 10-11 0.1019546984875106 1105 12-13 0.12722277125281778 1105 14-15 0.3243505386173382 1105 16-17 0.19851929589513873 1105 18-19 0.1698947568603515 1105 20-21 0.21484354052868326 1105 22-23 0.19583638965922745 1105 24-25 0.19716060732604035 1105 26-27 0.22300073126297804 1105 28-29 0.18776687623947197 1105 30-31 0.24758831037409834 1105 32-33 0.18531927155287065 1105 34-35 0.2452337346864013 1105 36-37 0.1783634539040193 1105 38-39 0.08354632202346579 1105 40-41 0.15473172034280225 1105 42-43 0.035354129065915174 1105 44-45 0.14032502504003475 1105 46-47 0.18470351502827498 1105 48-49 0.21307136968135865 1105 50-51 0.1745833129324197 1105 52-53 0.25699805652375574 1105 54-55 0.0691331491850633 1105 56-57 0.22208488342959498 1105 58-59 0.11146431110717714 1105 60-61 -0.12558292647170788 1105 62-63 -0.06986512602671269 1105 64-65 -0.09795922445760397 1105 66-67 -0.048401913516720185 1105 68-69 0.05493465845537315 1105 70-71 0.15384310665595535 1105 72-73 0.12225698348659364 1105 74-75 0.12665728773507823 1105 76-77 0.21394882100827672 1105 78-79 0.19320141303660776 1105 80-81 0.2635347056405841 1105 82-83 0.23889963557485672 1105 84-85 -0.0889459688428822 1105 86-87 -0.11999782379252721 1105 88-89 0.057117539581479804 1105 90-91 0.19728800298161886 1105 92-93 0.0034170896462626388 1105 94-95 0.030977399024255448 1105 96-97 -0.16742393855751558 1105 98-99 0.0405520515571105 1105 100-101 0.3971653720983568 1106 1 0.14410275428390662 1106 2 0.1056248998877436 1106 3 0.1172437274622169 1106 4 0.1383791034572326 1106 5 0.130546972258756 1106 6 0.15591751585127867 1106 7 0.14964942571596396 1106 8 0.15860534560083295 1106 9 0.12978598654745355 1106 10-11 0.10729921630404249 1106 12-13 0.13112042076816266 1106 14-15 0.12155202479161176 1106 16-17 0.1816044633318583 1106 18-19 0.11970721662277839 1106 20-21 0.1749012384154227 1106 22-23 0.2282881351013586 1106 24-25 0.24811258245653534 1106 26-27 0.3347368595381468 1106 28-29 0.30644007748058755 1106 30-31 0.2704299870865654 1106 32-33 0.15738455526116724 1106 34-35 0.24389223671401794 1106 36-37 0.28234238910157927 1106 38-39 0.38665012558706024 1106 40-41 0.38611959389167083 1106 42-43 0.34350507664095886 1106 44-45 0.33208176000407974 1106 46-47 0.4295787906648485 1106 48-49 0.28803999016585635 1106 50-51 0.48831730895845027 1106 52-53 0.438140599315048 1106 54-55 0.30337002168423055 1106 56-57 0.4402568334901602 1106 58-59 0.32234770815394853 1106 60-61 0.29744894315793147 1106 62-63 0.4322265960549956 1106 64-65 0.336680644811608 1106 66-67 0.4422280220989592 1106 68-69 0.48902662306778666 1106 70-71 0.46669971837566493 1106 72-73 0.4941468745456703 1106 74-75 0.49465047666438977 1106 76-77 0.41744966333931544 1106 78-79 0.5434610177368207 1106 80-81 0.5087391451314893 1106 82-83 0.5371216665421521 1106 84-85 0.4106878140003438 1106 86-87 0.4977778875150882 1106 88-89 0.7765354069365529 1106 90-91 0.7543647205811368 1106 92-93 0.6712674970431145 1106 94-95 0.8472154403866874 1106 96-97 0.7356820966254354 1106 98-99 0.9549669230684721 1106 100-101 0.7877500226836105 1107 1 -0.01642590975799152 1107 2 0.07654057242451984 1107 3 0.051539252214581666 1107 4 -0.025712838080103495 1107 5 -0.008026952403660914 1107 6 0.015046528860615638 1107 7 0.02955916896060984 1107 8 0.028981875770909937 1107 9 0.04606574851841572 1107 10-11 0.05918678076484696 1107 12-13 0.07940426649535226 1107 14-15 0.14280834398783782 1107 16-17 -0.010237548118276152 1107 18-19 0.07802675780883561 1107 20-21 0.06230767508332491 1107 22-23 0.020636632618369788 1107 24-25 -0.015598261072881314 1107 26-27 0.11944802922613462 1107 28-29 0.05083760110181146 1107 30-31 0.12068346265711938 1107 32-33 0.170250935185976 1107 34-35 0.2172993928315492 1107 36-37 0.2354722072959703 1107 38-39 0.1920732754054697 1107 40-41 0.2482643100315869 1107 42-43 0.11579684318905947 1107 44-45 0.020362718226863308 1107 46-47 0.06657439490446393 1107 48-49 0.16934273185938054 1107 50-51 0.08368846549900155 1107 52-53 0.0866781329293076 1107 54-55 0.022785981454909177 1107 56-57 0.011877558573253566 1107 58-59 -0.01674964745415508 1107 60-61 0.02656139750229869 1107 62-63 0.010073957476514295 1107 64-65 -0.01060201740275346 1107 66-67 0.19322838667671505 1107 68-69 0.19611386917516427 1107 70-71 0.117919921524134 1107 72-73 0.0677954244138661 1107 74-75 0.1925367344687956 1107 76-77 0.2507975892194203 1107 78-79 0.44332911863851976 1107 80-81 0.3837423551547694 1107 82-83 0.42750467944270554 1107 84-85 0.4163201663438265 1107 86-87 0.51387966759685 1107 88-89 0.6262478798434508 1107 90-91 0.7772065385199376 1107 92-93 0.8124580709184386 1107 94-95 0.6812340842019005 1107 96-97 0.43453115920764773 1107 98-99 0.6112734207419876 1107 100-101 0.8743525077572514 1108 1 -0.04894108660629115 1108 2 0.040663283869960765 1108 3 0.07717385056049864 1108 4 -7.785791467540548E-5 1108 5 0.03346690116129736 1108 6 0.06243300294261189 1108 7 0.05001381290723117 1108 8 0.06425627131721257 1108 9 -0.052781404740308346 1108 10-11 0.030924118723035576 1108 12-13 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-0.4874477013495735 2316 60-61 -0.33823857299930893 2316 62-63 -0.37115936816555006 2316 64-65 -0.5488931622992439 2316 66-67 -0.49649760840677715 2316 68-69 -0.48652003410071387 2316 70-71 -0.4350498914171226 2316 72-73 -0.5065512998240322 2316 74-75 -0.43907896723938933 2316 76-77 -0.45935893740343303 2316 78-79 -0.42054942980251653 2316 80-81 -0.5196439856427411 2316 82-83 -0.5071864223655353 2316 84-85 -0.7185305359094052 2316 86-87 -0.7138342766225385 2316 88-89 -0.8314347352207889 2316 90-91 -1.11539944594929 2316 92-93 -1.0796968233100408 2316 94-95 -0.880924830631006 2316 96-97 -0.8765472886297196 2316 98-99 -0.8135589078159455 2316 100-101 -0.06872969371769955 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 4.0 4 17.0 5 44.0 6 85.0 7 234.0 8 579.0 9 1126.0 10 1946.0 11 2899.0 12 4218.0 13 5970.0 14 7417.0 15 10403.0 16 13557.0 17 19348.0 18 18029.0 19 12448.0 20 11356.0 21 12239.0 22 13142.0 23 14416.0 24 16842.0 25 19971.0 26 23997.0 27 29274.0 28 35296.0 29 43971.0 30 53289.0 31 65588.0 32 80536.0 33 103771.0 34 141158.0 35 201675.0 36 318790.0 37 531406.0 38 456190.0 39 36523.0 40 2.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.34054105730184 20.09699139809711 16.12260373234372 22.439863812257332 2 15.277541858772143 21.00638714597705 40.04766526848545 23.66840572676535 3 19.021172045619995 25.21544701355427 30.12947620203141 25.633904738794328 4 13.198642839810033 17.6670451000104 37.22254480535238 31.91176725482719 5 13.95132076125767 37.65842201962076 33.72842063299476 14.661836586126805 6 30.98905432107325 36.585866814573436 17.70907720040212 14.716001663951191 7 27.97236281069089 33.13256144486429 20.75337123444379 18.141704510001038 8 25.983247824730476 35.110280098450446 19.923042257427113 18.983429819391965 9 26.490622941727043 17.306955662633897 20.480249246022115 35.722172149616945 10-11 25.147415675806844 27.067091031996394 26.812406836066145 20.97308645613062 12-13 26.018758449752138 24.362195375602315 27.43290030159115 22.186145873054393 14-15 22.924530284584158 25.51078443671525 26.296631646957653 25.268053631742944 16-17 22.064988507186555 28.78149744783493 26.815488616275662 22.33802542870285 18-19 21.997001421291642 27.315210420494335 29.24125558983603 21.446532568377993 20-21 23.100972371477102 26.29220542864076 29.0540177488127 21.552804451069434 22-23 22.640027559191598 26.326047769265436 28.930088050750513 22.103836620792457 24-25 22.417445326276557 26.53319227293497 29.064999652259978 21.984362748528497 26-27 22.19442434894369 26.70469970388657 29.071288870169198 22.029587077000542 28-29 21.937008181093354 26.941276389225916 29.233650812909488 21.888064616771242 30-31 22.225729711928448 26.564634104066283 29.07694040974798 22.13269577425729 32-33 22.0975318057337 26.640118556522342 28.890612542032102 22.371737095711858 34-35 22.60257203041508 26.792294048848387 28.65583958891887 21.949294331817665 36-37 22.708146560906066 26.460823135748424 28.788254081146835 22.042776222198672 38-39 22.318065256322335 26.674597009654 28.844650864483224 22.16268686954044 40-41 22.446082015405572 26.526742618253778 28.86234710253811 22.16482826380254 42-43 22.301678166159512 26.60467109659314 28.552008590602167 22.541642146645184 44-45 22.44374920105991 26.761968935042653 28.32446825797478 22.469813605922663 46-47 22.61211267306581 27.001678903298505 27.930979719914124 22.45522870372156 48-49 22.65658040697473 26.879354872257082 27.958409886643327 22.505654834124865 50-51 22.55711165805803 27.154903456165286 27.83669012375637 22.451294762020314 52-53 22.738499670676326 27.205168648386312 27.544632024127292 22.511699656810066 54-55 22.379406870731792 27.00040298817901 27.811470863521336 22.808719277567857 56-57 22.546196863234666 27.257238026752788 27.642308481451384 22.554256628561163 58-59 22.76190901259583 26.878736326496654 27.727312514191325 22.63204214671619 60-61 22.595402183295604 27.031218041828588 27.97440659633991 22.398973178535893 62-63 22.47143077520401 27.006215637198146 27.933902955634288 22.588450631963557 64-65 22.216304988387005 26.965802336464794 27.86583093562589 22.952061739522307 66-67 22.26860678753423 27.008224425416856 27.714905362776026 23.008263424272887 68-69 22.263756210869598 26.962773456466333 27.845776955331136 22.92769337733293 70-71 22.366168925711513 27.01316428051444 27.69503761223004 22.925629181544007 72-73 22.428328769022777 26.918527056539677 27.688732797171284 22.964411377266266 74-75 22.27573491177592 27.28275470586196 27.37758692411689 23.063923458245224 76-77 22.33403613954174 26.93944002364452 27.599306213222384 23.127217623591356 78-79 22.426248830034318 27.031775401254897 27.584129198876834 22.95784656983395 80-81 22.314296869346446 27.273999676738477 27.52249072573079 22.889212728184287 82-83 22.435153568828646 27.145327070405934 27.463991056262348 22.955528304503066 84-85 22.32578344368565 27.06293115401948 27.437190175754843 23.17409522654002 86-87 22.306237693272152 26.993763109193196 27.49278811379653 23.207211083738127 88-89 22.29458487553626 26.867564893195812 27.470994481444755 23.366855749823177 90-91 22.18936088467301 27.01299791286295 27.620157147351787 23.177484055112245 92-93 22.328015044892016 26.784869310500227 27.5887657295386 23.298349915069156 94-95 22.171889624571012 26.819296633965408 27.521449370818456 23.48736437064513 96-97 22.05084150864908 26.72457274586612 27.675928172773602 23.5486575727112 98-99 22.01647918327729 26.71592799944535 27.3706754601865 23.896917357090857 100-101 21.999444481998964 26.40101466532857 27.93122409821489 23.668316754457578 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 225.0 1 178.5 2 152.0 3 205.0 4 221.0 5 249.0 6 409.0 7 1024.5 8 1835.5 9 2238.5 10 2240.0 11 2104.5 12 2180.5 13 2372.5 14 2581.5 15 2767.0 16 2880.0 17 3023.5 18 3149.5 19 3307.0 20 3670.5 21 4272.0 22 4931.0 23 5940.0 24 7192.5 25 8693.5 26 11179.0 27 14128.0 28 16575.5 29 20040.5 30 24246.5 31 28356.5 32 33174.0 33 37742.5 34 42667.5 35 47456.5 36 52672.0 37 58523.0 38 62588.0 39 64708.5 40 67485.0 41 73633.5 42 78651.5 43 81482.0 44 84374.5 45 87445.0 46 88827.0 47 92228.0 48 103662.5 49 103583.0 50 93475.0 51 87110.5 52 81292.5 53 79080.0 54 76373.5 55 69575.5 56 61386.5 57 54969.0 58 49741.5 59 43200.0 60 36155.0 61 29602.0 62 23884.5 63 18458.0 64 14130.0 65 11118.0 66 8386.5 67 6865.0 68 5466.5 69 3713.0 70 2518.5 71 1923.5 72 1560.5 73 1245.5 74 979.0 75 807.0 76 565.0 77 318.0 78 195.0 79 123.5 80 73.0 81 49.0 82 25.0 83 12.0 84 10.0 85 6.5 86 3.0 87 0.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28274170624328354 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.00257825770444067 16-17 5.849828405033453E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 6.499809338926059E-5 26-27 0.001278295836655458 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.003856553541096128 36-37 0.02051773147987659 38-39 0.0072581204284674315 40-41 0.017896141713176415 42-43 0.005286511595659861 44-45 6.499809338926058E-4 46-47 1.516622179082747E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 2.166603112975353E-5 58-59 5.199847471140847E-4 60-61 0.0024265954865323952 62-63 0.0013866259923042258 64-65 0.0 66-67 0.0 68-69 4.983187159843312E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.2306782334384858 78-79 0.0 80-81 0.0013432939300447187 82-83 0.0 84-85 0.0 86-87 0.10245866121260444 88-89 0.3533513016951503 90-91 0.35525791243456856 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 1.7332824903802823E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2307760.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.59005960410007 #Duplication Level Percentage of deduplicated Percentage of total 1 70.07920412025479 34.05152705211075 2 15.628411250944465 15.187708684015593 3 5.885690363735276 8.579581367555233 4 2.825769692982315 5.492172712378808 5 1.5657029024550324 3.803879867630125 6 0.9640002055869272 2.810449646870011 7 0.593546747902835 2.018833030089296 8 0.4245954292471409 1.6504893771797622 9 0.29728919181397123 1.300076959490604 >10 1.5549067715226934 13.861893562611376 >50 0.11906560016591976 3.95744494439102 >100 0.05771210489145775 4.941431840933949 >500 0.003123840160979858 1.0001934139075954 >1k 8.032731842518157E-4 0.7415907461222486 >5k 1.7850515205595905E-4 0.6027267947138604 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7691 0.33326689083786876 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6207 0.2689621104447603 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4259 0.18455125316324056 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3225 0.13974590078691024 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.333206225950706E-5 0.0 10-11 0.0 0.0 0.0 4.333206225950706E-5 0.0 12-13 0.0 0.0 0.0 6.499809338926059E-5 0.0 14-15 0.0 0.0 0.0 8.666412451901411E-5 0.0 16-17 0.0 0.0 0.0 8.666412451901411E-5 0.0 18-19 0.0 0.0 0.0 8.666412451901411E-5 0.0 20-21 0.0 0.0 0.0 8.666412451901411E-5 0.0 22-23 0.0 0.0 0.0 1.2999618677852115E-4 0.0 24-25 0.0 0.0 0.0 2.1666031129753525E-4 0.0 26-27 0.0 0.0 0.0 4.983187159843312E-4 0.0 28-29 0.0 0.0 0.0 0.0011916317121364439 0.0 30-31 0.0 0.0 0.0 0.003661559260928346 0.0 32-33 0.0 0.0 0.0 0.01167799077893715 0.0 34-35 0.0 0.0 0.0 0.02322598537109578 0.0 36-37 0.0 0.0 0.0 0.04133878739556973 0.0 38-39 0.0 0.0 0.0 0.07206121953756023 0.0 40-41 0.0 0.0 0.0 0.10999844004575865 0.0 42-43 0.0 0.0 0.0 0.15064391444517627 0.0 44-45 0.0 0.0 0.0 0.1974425416854439 0.0 46-47 0.0 0.0 0.0 0.2593857246854092 0.0 48-49 0.0 0.0 0.0 0.3264420910319964 0.0 50-51 0.0 0.0 0.0 0.3998899365618609 0.0 52-53 0.0 0.0 0.0 0.47741099594411895 0.0 54-55 0.0 0.0 0.0 0.5680833362221376 0.0 56-57 0.0 0.0 0.0 0.6750918639719902 0.0 58-59 0.0 0.0 0.0 0.792976739348979 0.0 60-61 0.0 0.0 0.0 0.925551183831941 0.0 62-63 0.0 0.0 0.0 1.0652320865254619 0.0 64-65 0.0 0.0 0.0 1.2202742052899782 0.0 66-67 0.0 0.0 0.0 1.3989756300481853 0.0 68-69 0.0 0.0 0.0 1.595659860644088 0.0 70-71 0.0 0.0 0.0 1.8194049641210523 0.0 72-73 0.0 0.0 0.0 2.053246438104482 0.0 74-75 0.0 0.0 0.0 2.3063490137622633 0.0 76-77 0.0 0.0 0.0 2.585060838215412 0.0 78-79 0.0 0.0 0.0 2.90084324193157 0.0 80-81 0.0 0.0 0.0 3.252027940513745 0.0 82-83 0.0 0.0 0.0 3.6165155822095887 0.0 84-85 0.0 0.0 0.0 3.99610444760287 0.0 86-87 0.0 0.0 0.0 4.393286130273513 0.0 88-89 0.0 0.0 0.0 4.836941449717475 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTACGGT 90 9.0004096E-4 26.36366 4 GTATCAA 14750 0.0 25.712313 1 GTACATG 11860 0.0 23.070553 1 GGTATCA 10885 0.0 22.688917 1 TACATGG 11790 0.0 22.580173 2 ATCAACG 17680 0.0 21.418982 3 TCAACGC 17790 0.0 21.339893 4 GAGTACT 7630 0.0 21.334185 12-13 TATCAAC 17845 0.0 21.300716 2 AGTACTT 8110 0.0 21.241842 12-13 CAACGCA 17945 0.0 21.10268 5 ACATGGG 12045 0.0 20.683819 3 AACGCAG 18450 0.0 20.576515 6 CATGGGG 5410 0.0 19.911629 4 GTACTTT 8050 0.0 19.808418 14-15 ACGCAGA 20300 0.0 18.561054 7 CGCAGAG 20305 0.0 18.533113 8 GTCCCGA 385 1.2732926E-11 18.4888 6 AGAGTAC 16440 0.0 18.373972 10-11 TACTTTT 9340 0.0 18.190414 14-15 >>END_MODULE