##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939277_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 54787 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.922591125631993 31.0 31.0 34.0 30.0 34.0 2 32.094201179111835 33.0 31.0 34.0 30.0 34.0 3 32.128935696424335 34.0 31.0 34.0 30.0 34.0 4 35.710405753189626 37.0 35.0 37.0 33.0 37.0 5 35.65066530381295 37.0 35.0 37.0 33.0 37.0 6 35.80292770182707 37.0 35.0 37.0 33.0 37.0 7 35.75318962527607 37.0 35.0 37.0 33.0 37.0 8 35.78425538905215 37.0 35.0 37.0 33.0 37.0 9 37.27532078777812 39.0 37.0 39.0 34.0 39.0 10-11 37.22315512804133 39.0 37.0 39.0 33.5 39.0 12-13 37.18288097541387 39.0 37.0 39.0 33.0 39.0 14-15 38.03763666563236 40.0 37.0 41.0 33.0 41.0 16-17 38.08391589245623 40.0 37.5 41.0 33.0 41.0 18-19 38.33229598262361 40.0 37.0 41.0 34.0 41.0 20-21 38.352300728274955 40.0 37.5 41.0 34.0 41.0 22-23 38.293929216785 40.0 37.5 41.0 34.0 41.0 24-25 38.08958329530728 40.0 37.0 41.0 33.0 41.0 26-27 37.71715005384489 39.5 36.5 41.0 33.0 41.0 28-29 37.44379141037108 39.0 36.0 41.0 33.0 41.0 30-31 37.13448445799186 39.0 35.0 41.0 32.5 41.0 32-33 36.640672057239854 38.0 35.0 41.0 31.0 41.0 34-35 36.27402485991202 38.0 35.0 41.0 31.0 41.0 36-37 35.89872597514009 38.0 35.0 40.5 30.0 41.0 38-39 35.476235238286456 38.0 35.0 40.0 29.5 41.0 40-41 35.282475769799404 38.0 35.0 40.0 28.5 41.0 42-43 34.857393177213574 38.0 35.0 40.0 26.0 41.0 44-45 34.34732692061985 38.0 34.5 40.0 23.5 41.0 46-47 33.601319656122804 38.0 33.5 40.0 17.0 41.0 48-49 32.91147534999179 38.0 33.0 40.0 3.5 41.0 50-51 32.33551754978371 37.0 33.0 40.0 2.0 41.0 52-53 31.974665522843008 37.0 32.0 40.0 2.0 41.0 54-55 31.677213572562835 37.0 31.5 40.0 2.0 41.0 56-57 30.994715899757242 36.5 31.0 40.0 2.0 41.0 58-59 30.405862704656215 36.0 30.0 40.0 2.0 41.0 60-61 29.525206709620896 35.0 28.0 39.0 2.0 40.5 62-63 28.987250625148302 35.0 26.5 39.0 2.0 40.0 64-65 28.48879296183401 35.0 26.0 38.5 2.0 40.0 66-67 27.684760983444978 35.0 23.5 37.5 2.0 39.0 68-69 26.954879807253548 34.5 20.0 37.0 2.0 39.0 70-71 26.245432310584626 34.0 15.5 36.0 2.0 38.0 72-73 25.62179896690821 33.5 9.5 35.5 2.0 37.0 74-75 25.167494113567088 33.0 3.5 35.0 2.0 37.0 76-77 23.521537956084472 30.5 2.0 34.0 2.0 35.0 78-79 24.24832533265191 32.0 2.0 35.0 2.0 36.0 80-81 24.193878109770566 32.0 2.0 35.0 2.0 35.5 82-83 23.876786463942175 32.0 2.0 35.0 2.0 35.0 84-85 23.499233394783435 32.0 2.0 35.0 2.0 35.0 86-87 23.16912771277858 31.5 2.0 34.5 2.0 35.0 88-89 22.578239363352623 31.0 2.0 34.0 2.0 35.0 90-91 22.04026502637487 30.5 2.0 34.0 2.0 35.0 92-93 21.75871100808586 30.0 2.0 34.0 2.0 35.0 94-95 21.28134411447971 30.0 2.0 34.0 2.0 35.0 96-97 20.552585467355392 29.0 2.0 34.0 2.0 35.0 98-99 19.813769689889938 29.0 2.0 34.0 2.0 35.0 100-101 16.44371840035045 20.0 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2626932679810494 1101 2 0.22601886049991293 1101 3 -0.3509440591510149 1101 4 0.1886447659887338 1101 5 0.06738522392715396 1101 6 0.28374910498514794 1101 7 -0.15998970003176538 1101 8 0.45941932071294644 1101 9 0.5669161229770054 1101 10-11 0.33366476332582096 1101 12-13 0.17304473268079335 1101 14-15 -0.342865645000785 1101 16-17 0.10731618647780294 1101 18-19 0.3960067304217816 1101 20-21 -0.10652859479299792 1101 22-23 -0.1065646733883554 1101 24-25 0.3055661004649082 1101 26-27 -0.5125812653621722 1101 28-29 -0.19711954509102014 1101 30-31 -0.4226121840836754 1101 32-33 -0.47115378290121157 1101 34-35 -0.629917659060709 1101 36-37 -0.5047711379336235 1101 38-39 -0.5261518113130208 1101 40-41 0.1940878812708391 1101 42-43 -0.7214435156560839 1101 44-45 0.24271106283975286 1101 46-47 0.4490239500298401 1101 48-49 -0.7855238810936243 1101 50-51 -1.676511327791662 1101 52-53 -2.4963838063081525 1101 54-55 -2.0962312471329376 1101 56-57 -2.8864696449520046 1101 58-59 -3.1632892666795946 1101 60-61 -4.6746079074476015 1101 62-63 -4.697813516667232 1101 64-65 -4.185365773783463 1101 66-67 -4.2311082346736235 1101 68-69 -5.386138170903461 1101 70-71 -4.980988708382839 1101 72-73 -4.819250251550233 1101 74-75 -4.949361236097264 1101 76-77 -4.746815552688528 1101 78-79 -5.745535947173742 1101 80-81 -5.236190111290814 1101 82-83 -5.356354344651255 1101 84-85 -5.381196165592279 1101 86-87 -5.164876514547164 1101 88-89 -4.552770803300017 1101 90-91 -4.548648140889291 1101 92-93 -4.247994737954212 1101 94-95 -4.90199191828394 1101 96-97 -4.838917599225624 1101 98-99 -3.970605941376501 1101 100-101 -2.756568923326663 1102 1 0.2910266013143854 1102 2 0.028518860499914922 1102 3 0.014889274182316115 1102 4 0.40864476598873267 1102 5 0.3948852239271545 1102 6 0.35624910498514595 1102 7 0.512510299968234 1102 8 0.6869193207129456 1102 9 0.14941612297700857 1102 10-11 -0.0017519033408461837 1102 12-13 0.5059613993474628 1102 14-15 0.9467176883325408 1102 16-17 0.3348161864778021 1102 18-19 0.43225673042178414 1102 20-21 0.22097140520700265 1102 22-23 0.330101993278312 1102 24-25 -0.2236005662017604 1102 26-27 0.574502067971153 1102 28-29 0.7382971215756484 1102 30-31 0.9840544825829838 1102 32-33 0.5871795504321256 1102 34-35 1.3592490076059605 1102 36-37 1.7243955287330408 1102 38-39 2.2288481886869747 1102 40-41 1.7411712146041722 1102 42-43 2.0785564843439133 1102 44-45 2.013961062839755 1102 46-47 2.2211072833631675 1102 48-49 1.7832261189063772 1102 50-51 2.030155338875005 1102 52-53 1.5986161936918464 1102 54-55 1.8637687528670632 1102 56-57 1.6064470217146614 1102 58-59 0.9446273999870733 1102 60-61 1.4470587592190647 1102 62-63 1.5038531499994328 1102 64-65 1.7058842262165363 1102 66-67 2.443891765326377 1102 68-69 2.1555284957632033 1102 70-71 1.6727612916171637 1102 72-73 2.1549164151164284 1102 74-75 1.2960554305693996 1102 76-77 1.1323511139781353 1102 78-79 1.7161307194929272 1102 80-81 1.8575598887091864 1102 82-83 1.63406232201541 1102 84-85 2.0379705010743834 1102 86-87 2.3701234854528366 1102 88-89 1.5601458633666496 1102 90-91 2.463435192444045 1102 92-93 1.8524219287124524 1102 94-95 2.971758081716061 1102 96-97 3.001499067441042 1102 98-99 2.186477391956835 1102 100-101 0.016347743340002552 1103 1 0.5710266013143865 1103 2 0.30851886049991606 1103 3 0.6348892741823207 1103 4 0.5286447659887372 1103 5 0.41488522392715055 1103 6 0.5162491049851496 1103 7 0.43251029996823576 1103 8 0.12691932071294332 1103 9 0.12941612297700544 1103 10-11 0.21824809665915268 1103 12-13 0.25596139934746276 1103 14-15 0.8567176883325374 1103 16-17 0.38481618647780635 1103 18-19 0.642256730421785 1103 20-21 0.5609714052070061 1103 22-23 0.5901019932783171 1103 24-25 1.106399433798238 1103 26-27 1.0045020679711598 1103 28-29 1.118297121575651 1103 30-31 0.544054482582986 1103 32-33 1.4171795504321238 1103 34-35 0.749249007605961 1103 36-37 1.2143955287330357 1103 38-39 1.30884818868698 1103 40-41 1.9211712146041648 1103 42-43 1.6785564843439218 1103 44-45 1.303961062839754 1103 46-47 2.1711072833631704 1103 48-49 1.3032261189063803 1103 50-51 1.040155338875003 1103 52-53 0.9486161936918478 1103 54-55 0.7637687528670618 1103 56-57 1.0764470217146602 1103 58-59 1.1546273999870706 1103 60-61 1.5970587592190633 1103 62-63 1.943853149999434 1103 64-65 1.645884226216534 1103 66-67 -0.1861082346736218 1103 68-69 0.5355284957632058 1103 70-71 -0.16723870838283972 1103 72-73 0.2549164151164298 1103 74-75 -0.6139445694306005 1103 76-77 0.14235111397813682 1103 78-79 0.13613071949292888 1103 80-81 -0.16244011129081315 1103 82-83 -0.6259376779845915 1103 84-85 -0.4220294989256175 1103 86-87 -1.9398765145471621 1103 88-89 -1.3198541366333494 1103 90-91 -0.7565648075559572 1103 92-93 0.4924219287124494 1103 94-95 0.5517580817160592 1103 96-97 -0.5785009325589563 1103 98-99 0.006477391956835277 1103 100-101 0.9163477433400047 1104 1 0.4391117076973643 1104 2 0.32043375411693376 1104 3 -0.16255753432832165 1104 4 -0.024121191458071678 1104 5 0.14850224520375122 1104 6 -0.2228998311850674 1104 7 -0.16663863620198072 1104 8 -0.06584663673385904 1104 9 -0.28547749404427236 1104 10-11 -0.04643275440467676 1104 12-13 -0.17957051554616044 1104 14-15 -0.5075376308163939 1104 16-17 0.09268852690333063 1104 18-19 0.27566098574093445 1104 20-21 0.5277799158453007 1104 22-23 0.3579743337038437 1104 24-25 -0.03530269386133256 1104 26-27 -0.3674128256458644 1104 28-29 0.044680100299054004 1104 30-31 -0.36232849614041385 1104 32-33 0.0031369972406380953 1104 34-35 -0.6667084392025586 1104 36-37 0.44248063511602 1104 38-39 -0.2788113857811041 1104 40-41 -0.8043607002894504 1104 42-43 -0.35442223906033377 1104 44-45 -0.26327297971344166 1104 46-47 -0.5574033549347064 1104 48-49 -2.556135583221284 1104 50-51 -2.275802107933508 1104 52-53 -2.4405327424783643 1104 54-55 -1.6972950769201738 1104 56-57 -0.8295104250938508 1104 58-59 -1.976010897885267 1104 60-61 -1.824430602483062 1104 62-63 -1.4646574882984424 1104 64-65 -1.5264561993153798 1104 66-67 -1.6220656814821375 1104 68-69 -1.9561736318963732 1104 70-71 -1.706387644553054 1104 72-73 -1.6938069891388885 1104 74-75 -1.111816909856131 1104 76-77 -0.6080744179367592 1104 78-79 -0.9964224719964356 1104 80-81 -0.8179720261844317 1104 82-83 -1.8406185290484203 1104 84-85 -0.9499018393511456 1104 86-87 -0.5345573656109934 1104 88-89 -0.45325839195249884 1104 90-91 -0.5125222543644661 1104 92-93 -0.796088709585419 1104 94-95 -1.3241993650924542 1104 96-97 -0.4029690176653382 1104 98-99 -0.6073523952772071 1104 100-101 -0.9496097034685071 1105 1 -0.4089733986856139 1105 2 -0.05148113950009048 1105 3 0.37488927418231555 1105 4 -0.21135523401126477 1105 5 0.33488522392715225 1105 6 0.17624910498514623 1105 7 0.3925102999682366 1105 8 0.46691932071294673 1105 9 0.36941612297700743 1105 10-11 0.27824809665915495 1105 12-13 -0.08403860065254065 1105 14-15 0.3867176883325385 1105 16-17 0.1748161864777984 1105 18-19 0.26225673042178244 1105 20-21 -0.009028594793001332 1105 22-23 0.4001019932783194 1105 24-25 0.5463994337982356 1105 26-27 0.20450206797116266 1105 28-29 0.26829712157564956 1105 30-31 0.8240544825829872 1105 32-33 0.687179550432127 1105 34-35 0.5792490076059593 1105 36-37 0.5543955287330391 1105 38-39 0.3688481886869752 1105 40-41 0.04117121460416939 1105 42-43 0.4585564843439158 1105 44-45 -0.006038937160248281 1105 46-47 0.6311072833631712 1105 48-49 0.12322611890637347 1105 50-51 -0.2098446611249969 1105 52-53 -0.04138380630815419 1105 54-55 0.6037687528670652 1105 56-57 0.3764470217146574 1105 58-59 0.024627399987075194 1105 60-61 -0.4529412407809339 1105 62-63 0.25385314999943276 1105 64-65 -0.2541157737834645 1105 66-67 0.28389176532637705 1105 68-69 -0.17447150423679503 1105 70-71 0.7427612916171604 1105 72-73 0.6849164151164295 1105 74-75 -0.15394456943059964 1105 76-77 0.23235111397813668 1105 78-79 0.8261307194929266 1105 80-81 0.7275598887091874 1105 82-83 0.31406232201540973 1105 84-85 -0.3120294989256145 1105 86-87 -0.4698765145471633 1105 88-89 -0.18985413663335038 1105 90-91 0.4734351924440432 1105 92-93 -0.3375780712875489 1105 94-95 -0.39824191828394007 1105 96-97 -1.1085009325589574 1105 98-99 -1.7735226080431659 1105 100-101 -0.03365225665999816 1106 1 0.2341844960512276 1106 2 0.17448377278061145 1106 3 0.777345414533194 1106 4 0.08338160809399398 1106 5 -0.11353582870442835 1106 6 -0.09673335115520132 1106 7 -0.3036300509089571 1106 8 -0.3443087494624919 1106 9 0.05046875455595057 1106 10-11 0.18070423701003335 1106 12-13 -0.08737193398587095 1106 14-15 -0.6559138906148263 1106 16-17 0.268500397004118 1106 18-19 0.16629181814108307 1106 20-21 0.34412929994384456 1106 22-23 0.5688739231028777 1106 24-25 0.0018380302894698275 1106 26-27 -0.14251547588848723 1106 28-29 0.15759536718968548 1106 30-31 0.515984307144393 1106 32-33 0.22840762060756248 1106 34-35 1.0378454988340238 1106 36-37 0.8603604410137393 1106 38-39 1.1734095921957461 1106 40-41 1.5773115654813594 1106 42-43 2.054872273817601 1106 44-45 1.7081715891555405 1106 46-47 1.5230371079245728 1106 48-49 1.4595419083800607 1106 50-51 1.7708570932609717 1106 52-53 1.9224758428146558 1106 54-55 1.7941196300600488 1106 56-57 1.4715347410129063 1106 58-59 1.815329154373039 1106 60-61 0.9810938469383643 1106 62-63 1.5543794657889052 1106 64-65 2.1090421209533794 1106 66-67 2.9245935197123423 1106 68-69 2.7300898992719773 1106 70-71 2.7881998881083874 1106 72-73 2.258600625642746 1106 74-75 2.5807922726746604 1106 76-77 2.0305967280132258 1106 78-79 1.958411421247309 1106 80-81 1.9935248009898885 1106 82-83 1.8865184623662863 1106 84-85 1.9093740098463172 1106 86-87 1.7364392749265214 1106 88-89 2.182426565121034 1106 90-91 2.420101859110712 1106 92-93 2.547509648010699 1106 94-95 2.7838633448739536 1106 96-97 3.4522008218270095 1106 98-99 3.4301616024831496 1106 100-101 3.597049497725969 1107 1 -0.15860302831524464 1107 2 0.17740774938879866 1107 3 0.12822260751565295 1107 4 0.09605217339613858 1107 5 0.29192226096419205 1107 6 0.35550836424440746 1107 7 0.28213992959786083 1107 8 0.5358082096018322 1107 9 0.7960827896436697 1107 10-11 0.7908406892517519 1107 12-13 0.33855399194005287 1107 14-15 0.9707917624066198 1107 16-17 0.9985198901815053 1107 18-19 1.0337382119032625 1107 20-21 0.9976380718736664 1107 22-23 0.9489908821672017 1107 24-25 1.2152883226871225 1107 26-27 0.39598354945263736 1107 28-29 0.6697786030571322 1107 30-31 0.9292396677681793 1107 32-33 0.6445869578395289 1107 34-35 0.5622119705689173 1107 36-37 1.5577288620663694 1107 38-39 1.0525518923906816 1107 40-41 0.010060103493053418 1107 42-43 -0.054776848989412485 1107 44-45 -0.39270560382691144 1107 46-47 -0.1933371610812742 1107 48-49 0.525818711498971 1107 50-51 -1.4635483648286964 1107 52-53 -0.5287912137155573 1107 54-55 -1.1240090249107162 1107 56-57 -1.1839233486557106 1107 58-59 -1.1598170444573697 1107 60-61 -1.2440523518920443 1107 62-63 -1.0542949981487162 1107 64-65 -0.598560218227906 1107 66-67 -1.115367493932883 1107 68-69 0.6184914587261652 1107 70-71 0.12202055087642094 1107 72-73 0.80343493363495 1107 74-75 0.9082776527916216 1107 76-77 0.9360548176818391 1107 78-79 1.2405751639373683 1107 80-81 0.5983006294499269 1107 82-83 0.2003586183117072 1107 84-85 0.30167420477808804 1107 86-87 1.2471605224898745 1107 88-89 0.8997754929962802 1107 90-91 0.08676852577737648 1107 92-93 0.3920515583420823 1107 94-95 1.2287951187530979 1107 96-97 0.7085361044780818 1107 98-99 0.8175885030679453 1107 100-101 -0.14661521962295865 1108 1 -0.21765264396863415 1108 2 0.07153772842443828 1108 3 0.04017229305024017 1108 4 0.22638061504533624 1108 5 0.23601729939885274 1108 6 0.09624910498514794 1108 7 -0.03616894531478465 1108 8 -0.475722188721015 1108 9 -0.23850840532487894 1108 10-11 -0.16967643164272772 1108 12-13 -0.6785669025393375 1108 14-15 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-3.273392492243193 2316 82-83 -2.4610170430639577 2316 84-85 -1.8980612449573613 2316 86-87 -1.7898765145471636 2316 88-89 -2.7298541366333495 2316 90-91 -2.9529140139051613 2316 92-93 -2.9320225157319904 2316 94-95 -2.915384775426798 2316 96-97 -2.106278710336735 2316 98-99 -1.8054273699479282 2316 100-101 -0.8423824153901549 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 2.0 5 8.0 6 27.0 7 37.0 8 57.0 9 105.0 10 193.0 11 301.0 12 412.0 13 538.0 14 674.0 15 911.0 16 1147.0 17 1433.0 18 1387.0 19 1024.0 20 971.0 21 1127.0 22 1128.0 23 1085.0 24 1114.0 25 1039.0 26 958.0 27 912.0 28 1066.0 29 1174.0 30 1355.0 31 1661.0 32 2009.0 33 2510.0 34 3576.0 35 5328.0 36 6698.0 37 8906.0 38 3791.0 39 122.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.000292954445584 20.411600996045117 16.23883111176212 14.34927493774718 2 14.572800116816035 28.112508441783636 33.54262872579262 23.772062715607717 3 27.40248599120229 29.30439702849216 31.983864785441803 11.309252194863745 4 11.32932995053571 20.11243543176301 36.86823516527644 31.689999452424843 5 8.7338237173052 44.24772299998175 31.951010276160403 15.06744300655265 6 22.3501925639294 39.04393377991129 13.844525161078359 24.761348495080952 7 18.568273495537262 40.34716264807345 19.89340536988702 21.191158486502275 8 23.808567725920383 26.436928468432292 19.824045850292954 29.930457955354374 9 20.532243050358662 24.000219030061874 23.722780951685618 31.744756967893846 10-11 24.08326792852319 26.797415445269863 23.11223465420629 26.007081972000655 12-13 32.418274408162524 26.227024659134468 23.308449084636866 18.04625184806615 14-15 25.342922070218027 28.283427486698365 30.62159473592947 15.752055707154133 16-17 16.604303940715866 32.10889444576268 30.45795535437239 20.828846259149067 18-19 15.458959242155986 33.810940551590704 32.30419625093544 18.425903955317867 20-21 20.04216328691113 27.252815448920366 29.721466771314365 22.983554492854143 22-23 17.18564622994506 29.363717670250246 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19.285596948181137 28.41823790315221 32.76142150510157 19.53474364356508 50-51 14.566411740011317 30.418712468286273 36.43382554255572 18.581050249146696 52-53 17.213937612937375 32.44747841641265 30.627703652326282 19.71088031832369 54-55 14.71151915600416 28.42462627995692 32.19650647051308 24.66734809352584 56-57 19.979374480939647 28.458653135352684 29.114836684219654 22.447135699488012 58-59 22.135621064159896 28.107146116637765 30.10860637035685 19.648626448845487 60-61 17.783740371627786 31.031650421640567 32.7966998868324 18.387909319899247 62-63 16.14737341656628 31.31000620596503 31.688752601029456 20.853867776439238 64-65 15.12128789676383 35.09774216511216 29.517038713563437 20.263931224560572 66-67 17.12084983664008 29.978827094018655 28.07600343147097 24.8243196378703 68-69 15.755850034680394 30.29879896323878 30.03869601723068 23.906654984850146 70-71 20.178144450325807 29.93137058061219 27.373281982952157 22.517202986109844 72-73 18.175844634676107 26.20877215397813 32.682935732929344 22.932447478416414 74-75 15.70445543650866 32.891926916969354 28.320587000565826 23.083030645956157 76-77 17.745048712436535 26.77217216301514 29.71687325618625 25.765905868362072 78-79 17.446657053680617 27.692700823187984 31.610601054994795 23.2500410681366 80-81 19.351305966743936 27.167941299943415 29.736068775439428 23.74468395787322 82-83 16.79686787011517 26.780075565371348 32.62635296694471 23.796703597568765 84-85 16.847062259295086 28.47390804387902 29.995254348659355 24.683775348166538 86-87 17.18584426499068 28.880586107355576 28.958234369861515 24.97533525779223 88-89 17.154501327960435 28.399120798607928 26.86692920597124 27.579448667460387 90-91 18.081251715618997 25.643700247049132 28.565285021502422 27.70976301582945 92-93 19.036450252797195 28.80884151349773 28.72944311606768 23.425265117637394 94-95 17.917571686714002 28.039498421158303 28.390859145417707 25.652070746709988 96-97 14.323653421432091 28.587073575848287 30.435139722926973 26.654133279792653 98-99 17.754211765564822 29.429426688813038 25.506050705459327 27.31031084016281 100-101 17.047839816014747 27.49192326646832 27.04199901436472 28.41823790315221 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 883.0 1 527.5 2 194.5 3 224.0 4 165.0 5 133.5 6 233.0 7 456.0 8 595.5 9 544.5 10 394.5 11 250.5 12 220.0 13 218.0 14 210.5 15 190.0 16 177.5 17 182.5 18 204.0 19 221.5 20 222.0 21 240.0 22 277.0 23 307.0 24 365.0 25 445.5 26 529.0 27 599.5 28 638.5 29 708.0 30 838.0 31 915.5 32 896.5 33 855.5 34 809.5 35 767.5 36 702.5 37 585.0 38 495.0 39 465.5 40 452.5 41 463.5 42 528.5 43 602.5 44 619.0 45 711.0 46 1280.5 47 3674.0 48 8357.0 49 8525.0 50 4949.0 51 2990.0 52 1425.0 53 627.0 54 356.5 55 281.5 56 252.0 57 204.5 58 172.0 59 147.0 60 106.0 61 85.5 62 74.0 63 61.5 64 49.5 65 45.5 66 47.5 67 43.5 68 37.5 69 30.0 70 26.0 71 27.5 72 23.0 73 16.5 74 13.0 75 10.0 76 9.0 77 5.5 78 4.5 79 3.0 80 0.5 81 1.5 82 1.5 83 0.0 84 0.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31211783817328925 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 9.126252578166353E-4 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0045631262890831765 38-39 0.002737875773449906 40-41 0.0045631262890831765 42-43 0.0 44-45 0.0018252505156332705 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 9.126252578166353E-4 58-59 0.003650501031266541 60-61 0.0018252505156332705 62-63 0.0018252505156332705 64-65 0.0 66-67 0.0 68-69 0.0018252505156332705 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.23636994177450854 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.09673827732856334 88-89 0.35044809900158796 90-91 0.25918557321992447 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 54787.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.06235055761403 #Duplication Level Percentage of deduplicated Percentage of total 1 81.24786519412501 26.049975359118037 2 9.239439826938403 5.924763173745597 3 3.5181600819765455 3.3840144559840835 4 1.8558579073209611 2.380126672385785 5 1.0417852669930547 1.6701042218044426 6 0.5920528293293864 1.1389563217551608 7 0.41557554366389615 0.9327030134886014 8 0.20494136399863372 0.525672148502382 9 0.23340544233177732 0.6735174402686769 >10 1.1670272116588865 7.09839925529779 >50 0.14232039166571786 3.2124409075145564 >100 0.24479107366503472 17.945863069706316 >500 0.06831378799954457 15.368609341632139 >1k 0.028464078333143576 13.694854618796429 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2575 4.7000200777556715 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1555 2.838264551809736 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1246 2.274262142479055 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1097 2.002299815649698 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1030 1.8800080311022687 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 932 1.701133480570208 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 786 1.4346469052877506 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 776 1.4163944001314182 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 750 1.3689378867249529 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 727 1.3269571248653877 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 722 1.3178308722872214 No Hit CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT 708 1.2922773650683557 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 662 1.2083158413492252 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 626 1.1426068227864274 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 617 1.1261795681457278 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 558 1.018489787723365 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 556 1.0148392866920986 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 483 0.8815959990508697 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 460 0.8396152371913044 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 455 0.8304889846131381 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 450 0.8213627320349718 No Hit ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT 423 0.7720809681128734 RNA PCR Primer, Index 33 (95% over 22bp) TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 418 0.7629547155347072 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 416 0.7593042145034405 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 390 0.7118477010969755 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 361 0.6589154361436107 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 341 0.6224104258309453 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 317 0.5786044134557469 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 269 0.49099238870534984 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 241 0.4398853742676182 No Hit ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT 238 0.4344096227207184 RNA PCR Primer, Index 33 (95% over 24bp) ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 232 0.42345811962691876 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 232 0.42345811962691876 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 230 0.4198076185956522 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 227 0.4143318670487524 No Hit TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG 226 0.41250661653311915 RNA PCR Primer, Index 33 (96% over 29bp) CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 214 0.39060361034551994 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 213 0.3887783598298866 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 191 0.3486228484859547 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 190 0.34679759797032145 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 172 0.31394308868892257 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 167 0.3048168361107562 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 160 0.2920400825013233 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 151 0.2756128278606239 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 147 0.26831182579809076 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 143 0.2610108237355577 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 141 0.25736032270429116 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 135 0.24640881961049155 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 135 0.24640881961049155 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 127 0.2318068154854254 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 127 0.2318068154854254 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 119 0.2172048113603592 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 118 0.21537956084472593 No Hit CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCT 118 0.21537956084472593 RNA PCR Primer, Index 33 (96% over 27bp) ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 117 0.21355431032909267 No Hit TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC 112 0.2044280577509263 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 111 0.20260280723529306 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 107 0.19530180517275997 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 106 0.19347655465712668 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 102 0.1861755525945936 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 98 0.17887455053206053 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 97 0.17704930001642727 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 96 0.17522404950079398 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 91 0.16609779692262763 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 90 0.16427254640699435 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 79 0.14419479073502836 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 78 0.1423695402193951 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 76 0.13871903918812856 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 76 0.13871903918812856 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 71 0.12959278660996223 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 69 0.1259422855786957 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 68 0.12411703506306242 No Hit CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTG 68 0.12411703506306242 RNA PCR Primer, Index 15 (95% over 24bp) CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 64 0.11681603300052931 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 63 0.11499078248489605 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 63 0.11499078248489605 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 62 0.1131655319692628 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 62 0.1131655319692628 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 61 0.11134028145362951 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 61 0.11134028145362951 No Hit TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA 57 0.10403927939109643 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0018252505156332707 0.0 3 0.0 0.0 0.0 0.0018252505156332707 0.0 4 0.0 0.0 0.0 0.0018252505156332707 0.0 5 0.0 0.0 0.0 0.0018252505156332707 0.0 6 0.0 0.0 0.0 0.0018252505156332707 0.0 7 0.0 0.0 0.0 0.0018252505156332707 0.0 8 0.0 0.0 0.0 0.0018252505156332707 0.0 9 0.0 0.0 0.0 0.0018252505156332707 0.0 10-11 0.0 0.0 0.0 0.0018252505156332707 0.0 12-13 0.0 0.0 0.0 0.0036505010312665414 0.0 14-15 0.0 0.0 0.0 0.0036505010312665414 0.0 16-17 0.0 0.0 0.0 0.0036505010312665414 0.0 18-19 0.0 0.0 0.0 0.0036505010312665414 0.0 20-21 0.0 0.0 0.0 0.0045631262890831765 0.0 22-23 0.0 0.0 0.0 0.010951503093799624 0.0 24-25 0.0 0.0 0.0 0.028291382992315695 0.0 26-27 0.0 0.0 0.0 0.06023326701589793 0.0 28-29 0.0 0.0 0.0 0.16974829795389418 0.0 30-31 0.0 0.0 0.0 0.40064248818150294 0.0 32-33 0.0 0.0 0.0 0.7456148356361911 0.0 34-35 0.0 0.0 0.0 1.2329567233102745 0.0 36-37 0.0 0.0 0.0 1.784182379031522 0.0 38-39 0.0 0.0 0.0 2.3910781754795845 0.0 40-41 0.0 0.0 0.0 3.0171391023417966 0.0 42-43 0.0 0.0 0.0 3.5784036358990274 0.0 44-45 0.0 0.0 0.0 4.100425283370143 0.0 46-47 0.0 0.0 0.0 4.704583204044756 0.0 48-49 0.0 0.0 0.0 5.3379451329695 0.0 50-51 0.0 0.0 0.0 5.897384416011097 0.0 52-53 0.0 0.0 0.0 6.329968788216183 0.0 54-55 0.0 0.0 0.0 6.807271798054282 0.0 56-57 0.0 0.0 0.0 7.468925109971344 0.0 58-59 0.0 0.0 0.0 7.979995254348659 0.0 60-61 0.0 0.0 0.0 8.564075419351306 0.0 62-63 0.0 0.0 0.0 9.079708690017704 0.0 64-65 0.0 0.0 0.0 9.591691459652838 0.0 66-67 0.0 0.0 0.0 10.224140763319767 0.0 68-69 0.0 0.0 0.0 10.963367222151241 0.0 70-71 0.0 0.0 0.0 11.68799167685765 0.0 72-73 0.0 0.0 0.0 12.313139978462043 0.0 74-75 0.0 0.0 0.0 13.213901107927063 0.0 76-77 0.0 0.0 0.0 13.86551554200814 0.0 78-79 0.0 0.0 0.0 14.854801321481375 0.0 80-81 0.0 0.0 0.0 15.79115483600124 0.0 82-83 0.0 0.0 0.0 16.521255042254552 0.0 84-85 0.0 0.0 0.0 17.440268676875903 0.0 86-87 0.0 0.0 0.0 18.290835417161006 0.0 88-89 0.0 0.0 0.0 19.187946045594757 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTACTC 55 4.9393664E-5 43.125366 7 TGGGGAA 45 9.606499E-4 42.167023 8 CATGGGG 135 1.1685188E-8 31.625267 6 ATGGGGA 80 4.4727264E-4 29.64869 5 CTTCTGT 35 0.008412548 27.10737 94-95 GAGTACT 705 0.0 24.560047 12-13 GTACTTT 715 0.0 24.216549 14-15 ACTTTTT 785 0.0 22.057112 16-17 AGTACTT 675 0.0 21.434904 12-13 CTTGAAA 295 0.0 20.100805 56-57 TACTTTT 725 0.0 19.956635 14-15 TGCTTGA 305 0.0 19.441763 54-55 TCTGCTT 380 0.0 16.852938 52-53 CTTCTGC 385 0.0 16.63407 50-51 GCTTGAA 300 0.0 16.603264 56-57 TGAAAAA 350 0.0 16.264423 58-59 TTGAAAA 310 0.0 16.067675 58-59 GCAGAGA 115 7.98303E-4 14.437622 94-95 GTCTTCT 455 0.0 14.074981 48-49 TTCTGCT 390 0.0 13.988098 52-53 >>END_MODULE