##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939276_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 55282 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.890633479251836 31.0 31.0 34.0 30.0 34.0 2 32.03786042473138 33.0 31.0 34.0 30.0 34.0 3 32.08675518251872 33.0 31.0 34.0 30.0 34.0 4 35.67797836547158 37.0 35.0 37.0 33.0 37.0 5 35.63821858832893 37.0 35.0 37.0 33.0 37.0 6 35.77403133027025 37.0 35.0 37.0 33.0 37.0 7 35.725733511812166 37.0 35.0 37.0 33.0 37.0 8 35.76721175066025 37.0 35.0 37.0 33.0 37.0 9 37.28644043269057 39.0 37.0 39.0 34.0 39.0 10-11 37.2052295503057 39.0 37.0 39.0 33.5 39.0 12-13 37.150356354690494 39.0 37.0 39.0 33.0 39.0 14-15 38.01255381498498 40.0 37.0 41.0 33.0 41.0 16-17 38.03616005209652 40.0 37.0 41.0 33.0 41.0 18-19 38.28240656995044 40.0 37.0 41.0 33.5 41.0 20-21 38.34599869758692 40.0 37.5 41.0 34.0 41.0 22-23 38.26376578271408 40.0 37.0 41.0 33.5 41.0 24-25 38.085235700589706 40.0 37.0 41.0 33.0 41.0 26-27 37.6703719112912 39.5 36.5 41.0 32.5 41.0 28-29 37.40943706812344 39.0 36.0 41.0 32.5 41.0 30-31 37.09283311023479 39.0 35.0 41.0 32.0 41.0 32-33 36.56249773886618 38.0 35.0 41.0 31.0 41.0 34-35 36.1693770124091 38.0 35.0 40.0 30.5 41.0 36-37 35.854093556673064 38.0 35.0 40.5 30.0 41.0 38-39 35.43718570239861 38.0 35.0 40.0 29.5 41.0 40-41 35.25862848666836 38.0 35.0 40.0 28.5 41.0 42-43 34.85004160486234 38.0 35.0 40.0 26.0 41.0 44-45 34.32781013711515 38.0 34.5 40.0 23.5 41.0 46-47 33.58359863970189 38.0 33.5 40.0 16.0 41.0 48-49 32.885930320900115 38.0 33.0 40.0 2.0 41.0 50-51 32.33141890669657 37.0 33.0 40.0 2.0 41.0 52-53 32.01844180745993 37.0 32.5 40.0 2.0 41.0 54-55 31.674686154625377 37.0 32.0 40.0 2.0 41.0 56-57 31.0693987192938 36.5 31.0 40.0 2.0 41.0 58-59 30.51701277088383 36.0 30.5 40.0 2.0 41.0 60-61 29.69730653739011 35.0 28.0 39.0 2.0 40.5 62-63 29.09732824427481 35.0 27.0 39.0 2.0 40.0 64-65 28.43672443109873 35.0 26.0 38.5 2.0 40.0 66-67 27.603966933178974 35.0 22.0 37.5 2.0 39.5 68-69 27.01324119966716 34.5 20.5 37.0 2.0 39.0 70-71 26.38851705799356 34.0 16.0 36.0 2.0 38.5 72-73 25.780751419992043 33.5 10.0 35.5 2.0 37.0 74-75 25.356237111537208 33.0 4.5 35.0 2.0 37.0 76-77 23.701901161318332 30.5 2.0 34.0 2.0 35.0 78-79 24.463098296009548 32.5 2.0 35.0 2.0 36.0 80-81 24.39388046742158 32.5 2.0 35.0 2.0 36.0 82-83 24.113753120364677 32.5 2.0 35.0 2.0 35.0 84-85 23.791315437212837 32.0 2.0 35.0 2.0 35.0 86-87 23.490204768279007 32.0 2.0 35.0 2.0 35.0 88-89 22.975941536123873 31.5 2.0 34.5 2.0 35.0 90-91 22.503726348540212 31.0 2.0 34.0 2.0 35.0 92-93 22.24498932744836 31.0 2.0 34.0 2.0 35.0 94-95 21.898457002279223 31.0 2.0 34.0 2.0 35.0 96-97 21.35640895770775 30.0 2.0 34.0 2.0 35.0 98-99 20.77682609167541 30.0 2.0 34.0 2.0 35.0 100-101 18.13082015846026 24.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5004294506186788 1101 2 0.6499751781668905 1101 3 0.5521460714929489 1101 4 0.5391085711830144 1101 5 0.4575699187987965 1101 6 0.5307109110021102 1101 7 0.5536445276364503 1101 8 0.7842781468849012 1101 9 0.46968417595248724 1101 10-11 0.42608885053358136 1101 12-13 0.5299123487377244 1101 14-15 0.857928422316526 1101 16-17 0.9223096779374487 1101 18-19 0.753264771973484 1101 20-21 0.5285084182649626 1101 22-23 0.7435500341580337 1101 24-25 0.2319110279712504 1101 26-27 0.6383628841451383 1101 28-29 0.43586119424980296 1101 30-31 0.8004274700856868 1101 32-33 0.9530305599369626 1101 34-35 1.0902783443261157 1101 36-37 1.0580061340639446 1101 38-39 1.69808960078646 1101 40-41 2.0182500260903637 1101 42-43 1.7165373856337283 1101 44-45 0.7825981557524813 1101 46-47 0.5541835289333505 1101 48-49 0.05219326172736061 1101 50-51 -0.5486939437582947 1101 52-53 0.08781733114643941 1101 54-55 -0.5666538049037833 1101 56-57 -1.110341818257183 1101 58-59 -0.554526859582122 1101 60-61 0.03712812239726304 1101 62-63 -0.23476615612687368 1101 64-65 -1.2440217880554911 1101 66-67 -1.6717647744403514 1101 68-69 -1.173981703164035 1101 70-71 -0.8549512640054608 1101 72-73 -0.28416759866308894 1101 74-75 -0.09191238494977938 1101 76-77 0.023195952412759624 1101 78-79 -0.6131184565923462 1101 80-81 -1.4932391854445761 1101 82-83 -1.764879156469597 1101 84-85 -2.137436658647733 1101 86-87 -2.685327926302154 1101 88-89 -2.3074433955165645 1101 90-91 -2.717747175726256 1101 92-93 -2.962152910309168 1101 94-95 -2.3014525972188373 1101 96-97 -2.6861690888794207 1101 98-99 -2.37098067717292 1101 100-101 -0.6867936984731955 1102 1 0.01626278395200842 1102 2 0.011641844833555126 1102 3 -0.2520205951737218 1102 4 -0.3050580954836519 1102 5 0.13006991879879592 1102 6 -0.5826224223312266 1102 7 0.004477860969785752 1102 8 -0.16988851978176456 1102 9 0.12801750928582578 1102 10-11 0.026922183866908256 1102 12-13 -0.0013376512622755854 1102 14-15 0.4583450889832008 1102 16-17 0.07980967793744753 1102 18-19 0.21409810530681028 1102 20-21 -0.12107491506836254 1102 22-23 0.09980003415803651 1102 24-25 -0.8372556386954173 1102 26-27 -0.7445537825215354 1102 28-29 -0.3078888057501956 1102 30-31 -0.5270725299143137 1102 32-33 -1.3682194400630365 1102 34-35 -0.4088883223405517 1102 36-37 -1.329077199269392 1102 38-39 -0.647327065880205 1102 40-41 -1.1209166405763042 1102 42-43 -0.5492959476996049 1102 44-45 -1.4203185109141785 1102 46-47 -0.7437331377333152 1102 48-49 -0.0019734049393065334 1102 50-51 0.0013060562417024357 1102 52-53 -0.4530160021868923 1102 54-55 -1.264570471570451 1102 56-57 -1.5057584849238488 1102 58-59 -1.4382768595821211 1102 60-61 -2.055371877602738 1102 62-63 -2.79809948946021 1102 64-65 -1.7231884547221554 1102 66-67 -1.8096814411070135 1102 68-69 -2.0023150364973716 1102 70-71 -1.8937012640054611 1102 72-73 -3.0120842653297544 1102 74-75 -2.7448290516164455 1102 76-77 -3.0913873809205725 1102 78-79 -3.389368456592347 1102 80-81 -2.9053225187779113 1102 82-83 -3.091962489802931 1102 84-85 -2.442853325314399 1102 86-87 -2.6607445929688183 1102 88-89 -2.276610062183231 1102 90-91 -2.25983050905959 1102 92-93 -3.5263195769758333 1102 94-95 -3.6472859305521723 1102 96-97 -3.5478357555460853 1102 98-99 -4.321814010506255 1102 100-101 -3.129710365139859 1103 1 -0.35783141894654236 1103 2 0.36127952599297686 1103 3 0.4391025932320787 1103 4 0.1904129190090984 1103 5 0.05670035358140524 1103 6 0.6637543892629765 1103 7 0.4292967015494966 1103 8 0.2929737990588137 1103 9 0.387075480300318 1103 10-11 0.636523633142275 1103 12-13 0.25817321830294304 1103 14-15 0.18705885709913872 1103 16-17 0.8162227214157127 1103 18-19 1.0302212937126072 1103 20-21 0.72720407043888 1103 22-23 1.0522456863319434 1103 24-25 1.0440849410147308 1103 26-27 1.0674933189277453 1103 28-29 1.0358611942498044 1103 30-31 1.2908622526943816 1103 32-33 1.0978131686326194 1103 34-35 1.1246261704130731 1103 36-37 0.9893104818900298 1103 38-39 0.3467852529603732 1103 40-41 1.3039022000034137 1103 42-43 1.368276516068505 1103 44-45 2.3808590253177044 1103 46-47 2.71505309415074 1103 48-49 2.7217584791186624 1103 50-51 1.8165234475460466 1103 52-53 2.1330347224507875 1103 54-55 1.7485635864005609 1103 56-57 2.684006007829769 1103 58-59 2.046777488243965 1103 60-61 0.6158237745711759 1103 62-63 0.16566862648182124 1103 64-65 1.3103260380314659 1103 66-67 1.9643221820813892 1103 68-69 2.1951487316185734 1103 70-71 2.0533096055597575 1103 72-73 2.011919357858652 1103 74-75 1.2085223976589177 1103 76-77 1.153630735021455 1103 78-79 0.21253371732069581 1103 80-81 0.8315434232510768 1103 82-83 1.6503382348347557 1103 84-85 1.0838676891783514 1103 86-87 1.507280769350018 1103 88-89 1.4634261697008242 1103 90-91 1.346600650360699 1103 92-93 0.40393404621256934 1103 94-95 0.04202566365072613 1103 96-97 0.6786135198162313 1103 98-99 0.20988888804447114 1103 100-101 -0.2524458723862395 1104 1 0.806703960422599 1104 2 0.7935045899315938 1104 3 0.9697931303164751 1104 4 0.5932262182418384 1104 5 0.3371777619360543 1104 6 0.603652087472696 1104 7 0.7238406060678244 1104 8 0.7529055978652934 1104 9 1.022625352423077 1104 10-11 0.9227555172002511 1104 12-13 1.142657446776937 1104 14-15 1.0067519517282975 1104 16-17 0.7746626191139185 1104 18-19 1.1050294778558367 1104 20-21 1.442037830029669 1104 22-23 1.3504127792560752 1104 24-25 1.5291659299320344 1104 26-27 2.122480531203962 1104 28-29 1.5260572726811787 1104 30-31 1.5868980583209762 1104 32-33 1.5698933050350021 1104 34-35 2.2712587364829844 1104 36-37 2.264672800730615 1104 38-39 2.73318764000215 1104 40-41 2.6572696339335025 1104 42-43 2.7435962091631367 1104 44-45 2.770245214576015 1104 46-47 3.042418823051001 1104 48-49 1.5825854185901065 1104 50-51 2.364051154280915 1104 52-53 2.7295820370287913 1104 54-55 2.6637383519589655 1104 56-57 2.8600503386055642 1104 58-59 1.4054731404178789 1104 60-61 1.3257555733776556 1104 62-63 1.7362142360299906 1104 64-65 1.9569586041013736 1104 66-67 2.7803920883047475 1104 68-69 2.8285673164438094 1104 70-71 2.624068343837674 1104 72-73 1.5877931856506358 1104 74-75 1.1074993797561064 1104 76-77 0.851627324961779 1104 78-79 1.4874697787017688 1104 80-81 1.4898980694573858 1104 82-83 1.9037482945107982 1104 84-85 2.584720204097362 1104 86-87 2.1511426619331395 1104 88-89 2.060399741738337 1104 90-91 2.0275469419208 1104 92-93 2.4209843445927923 1104 94-95 1.7240375988595922 1104 96-97 2.6016740483754823 1104 98-99 2.792156577729042 1104 100-101 1.693206301526807 1105 1 0.4145470976775023 1105 2 0.4405634134610068 1105 3 0.06783234600275279 1105 4 0.02459876726144472 1105 5 -0.07458694394630072 1105 6 0.3487501266883868 1105 7 0.2924680570482181 1105 8 0.39996442139470645 1105 9 -0.09502170640045193 1105 10-11 0.24628492896494691 1105 12-13 0.6524613683455698 1105 14-15 0.9381245007479038 1105 16-17 0.5197606583296022 1105 18-19 -0.08124503194808597 1105 20-21 0.21654763395123666 1105 22-23 0.35041277925607517 1105 24-25 0.4703424005202663 1105 26-27 0.8185589625765104 1105 28-29 0.7515474687596111 1105 30-31 0.45944707792882156 1105 32-33 1.4424423246428475 1105 34-35 1.3202783443261197 1105 36-37 1.382319859554137 1105 38-39 0.497893522355092 1105 40-41 1.2258970849138962 1105 42-43 2.2730079738690137 1105 44-45 2.348676587125034 1105 46-47 2.9443796073647235 1105 48-49 2.9845462029038288 1105 50-51 2.275815860163277 1105 52-53 2.7786016448719337 1105 54-55 3.585306979409946 1105 56-57 2.3796581817428155 1105 58-59 3.111355493359053 1105 60-61 3.6002653772992232 1105 62-63 3.844057373284894 1105 64-65 2.976566447238625 1105 66-67 2.349019539285141 1105 68-69 2.2501359438947865 1105 70-71 2.820146775210226 1105 72-73 2.6760284797682843 1105 74-75 2.0388719287757127 1105 76-77 2.7437841877068756 1105 78-79 2.595312915956672 1105 80-81 1.823231402790718 1105 82-83 2.295905157255895 1105 84-85 2.4768770668424622 1105 86-87 2.5825152109527494 1105 88-89 2.4721644476206883 1105 90-91 2.8510763536855066 1105 92-93 3.2052980700829856 1105 94-95 3.9005081870948857 1105 96-97 4.6703014993558725 1105 98-99 4.007842852238845 1105 100-101 2.6834023799581814 1106 1 -0.8026474724582471 1106 2 -0.6838709756792625 1106 3 0.005992225339099377 1106 4 -0.3739683518939074 1106 5 -0.5478146965858173 1106 6 -0.7492890889978909 1106 7 -0.5740477800558565 1106 8 -0.7387987761920201 1106 9 -0.3880081317398165 1106 10-11 -0.44583422638950054 1106 12-13 -0.7970107281853558 1106 14-15 -1.2539946546065508 1106 16-17 -0.011536475908705768 1106 18-19 -0.4605813818726716 1106 20-21 0.36850841826496605 1106 22-23 0.3570115726195766 1106 24-25 0.7092187202789404 1106 26-27 0.049516730298982736 1106 28-29 0.343553501942111 1106 30-31 -0.051110991452773646 1106 32-33 0.8361074830138833 1106 34-35 0.17604757509534608 1106 36-37 0.6941599802177905 1106 38-39 1.0457819084787658 1106 40-41 0.7140192568596007 1106 42-43 0.05807584717219072 1106 44-45 0.5083673865217193 1106 46-47 1.213798913548736 1106 48-49 0.40988556941966436 1106 50-51 0.09745990239554914 1106 52-53 -0.5091057457766368 1106 54-55 -0.9801153433653234 1106 56-57 -2.1588033567187246 1106 58-59 -2.2868345518898145 1106 60-61 -3.156333416064278 1106 62-63 -1.7051507715114873 1106 64-65 -0.7920987111324145 1106 66-67 -1.467534005209579 1106 68-69 -1.6401355493178826 1106 70-71 -1.4537974178516144 1106 72-73 -0.9199368294323236 1106 74-75 -1.2872970003343909 1106 76-77 -1.5825732783564703 1106 78-79 -1.2908107642846538 1106 80-81 -1.3628545700599588 1106 82-83 -0.895648387238829 1106 84-85 -1.0574366586477346 1106 86-87 -0.28494331091754077 1106 88-89 -1.36475108782426 1106 90-91 -0.7654394834185645 1106 92-93 -0.422152910309169 1106 94-95 -1.0976064433726869 1106 96-97 -1.7080921658024977 1106 98-99 -1.4452114464036896 1106 100-101 -0.08563985231934623 1107 1 -0.2795705493813223 1107 2 -0.2118430036512926 1107 3 -0.07512665577978339 1107 4 -0.009982337907899819 1107 5 0.20847900970788658 1107 6 0.23252909282029322 1107 7 -0.4027191087271831 1107 8 0.3660963287030796 1107 9 0.46968417595248724 1107 10-11 -0.016638422193693714 1107 12-13 -0.12008765126228127 1107 14-15 0.31156478595288917 1107 16-17 0.27412785975563025 1107 18-19 -0.09764431893560754 1107 20-21 0.30032660008314593 1107 22-23 0.09355003415803509 1107 24-25 -0.7899071538469329 1107 26-27 -0.18981893403667982 1107 28-29 -0.7277751693865611 1107 30-31 -1.4986634390052203 1107 32-33 -1.2869694400630394 1107 34-35 -1.0524489284011551 1107 36-37 -1.0201756841178735 1107 38-39 -2.0028194901226257 1107 40-41 -2.4190227011823637 1107 42-43 -2.430735341639007 1107 44-45 -1.6019472987929646 1107 46-47 -0.9912710165211962 1107 48-49 -1.525079465545371 1107 50-51 -1.8350575801219335 1107 52-53 -1.8549099415808286 1107 54-55 -1.3984719867219653 1107 56-57 -1.8385236364390032 1107 58-59 -1.2127086777639384 1107 60-61 -0.8701446048754669 1107 62-63 -1.632947974308692 1107 64-65 -0.5485672426009458 1107 66-67 -1.4390375017130772 1107 68-69 -1.5530726122549439 1107 70-71 -2.14313308218728 1107 72-73 -2.150531235026726 1107 74-75 -1.7310032940406863 1107 76-77 -0.7995313203145109 1107 78-79 -1.5740275475014407 1107 80-81 -1.7986937308991209 1107 82-83 -1.9376064291968689 1107 84-85 -2.1256184768295547 1107 86-87 -1.8798733808476094 1107 88-89 -1.6838070318802032 1107 90-91 -1.2532017211808046 1107 92-93 -1.3494256375818985 1107 94-95 -1.6469071426733883 1107 96-97 -0.7243509070612397 1107 98-99 -0.2600715862638303 1107 100-101 0.7841153924359006 1108 1 -0.1341160039267777 1108 2 0.006338814530529646 1108 3 -0.29330847396159854 1108 4 -0.4099823379078984 1108 5 -0.9915209902921163 1108 6 -0.05837999808880312 1108 7 -0.12999183599991682 1108 8 -0.3248127622060082 1108 9 0.41513872140703256 1108 10-11 -0.0439111494664175 1108 12-13 -0.474633105807726 1108 14-15 -0.41570794131983035 1108 16-17 -0.25314486751709353 1108 18-19 -0.9430988643901586 1108 20-21 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2315 76-77 2.4997584524127596 2315 78-79 2.860631543407653 2315 80-81 3.3108233145554244 2315 82-83 3.1866833435304045 2315 84-85 2.6925633413522654 2315 86-87 2.763734573697846 2315 88-89 2.7728691044834335 2315 90-91 2.300065324273742 2315 92-93 1.937222089690831 2315 94-95 2.38917240278116 2315 96-97 2.587580911120579 2315 98-99 3.1208943228270805 2315 100-101 4.122893801526807 2316 1 -0.5358205493813237 2316 2 -1.1393998218331092 2316 3 -0.5541039285070539 2316 4 -0.7633914288169876 2316 5 -0.3855550812012041 2316 6 -0.6242890889978909 2316 7 -0.67260547236355 2316 8 -1.0032218531151003 2316 9 -1.54906582404751 2316 10-11 -0.6267236494664203 2316 12-13 -1.0247751512622756 2316 14-15 -0.7239465776834706 2316 16-17 -1.1389403220625525 2316 18-19 -1.3926727280265183 2316 20-21 -1.592429081735034 2316 22-23 -1.1970749658419635 2316 24-25 -0.865901472028753 2316 26-27 -0.812262115854864 2316 28-29 -0.9797638057501956 2316 30-31 -1.4020725299143137 2316 32-33 -1.4385319400630365 2316 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-0.6212931771729195 2316 100-101 0.7557063015268071 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 2.0 5 6.0 6 23.0 7 34.0 8 60.0 9 113.0 10 193.0 11 310.0 12 409.0 13 548.0 14 662.0 15 920.0 16 1199.0 17 1527.0 18 1441.0 19 961.0 20 958.0 21 1061.0 22 1075.0 23 1042.0 24 1076.0 25 1053.0 26 975.0 27 857.0 28 1001.0 29 1196.0 30 1411.0 31 1620.0 32 1902.0 33 2423.0 34 3171.0 35 5198.0 36 6409.0 37 9358.0 38 4878.0 39 209.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.271813845512085 20.657634619221213 15.720840814697933 14.349710720568766 2 14.47306537390109 27.86078651278897 33.97127455591332 23.69487355739662 3 27.52252089287652 29.476140515900294 31.436995767157484 11.564342824065701 4 11.450381679389313 20.225389819471076 36.907130711624035 31.417097789515573 5 8.863644585941174 43.79906660395789 31.945298650555337 15.391990159545601 6 22.756050794110198 38.808292029955496 14.044354401070875 24.391302774863426 7 18.40201150464889 40.57378531891032 19.977569552476393 21.0466336239644 8 23.69849137151333 26.61987627075721 19.83828370898303 29.843348648746428 9 21.086429579248218 23.89385333381571 23.882999891465577 31.1367171954705 10-11 24.007814478492094 26.831518396584787 23.084367425201695 26.076299699721428 12-13 32.40114322926088 26.034694837379256 23.62161282153323 17.942549111826633 14-15 25.43731119191042 28.07564986161611 30.83428302671804 15.652755919755432 16-17 16.63018704098983 31.925400672913423 30.552440215621722 20.891972070475017 18-19 15.563836330089359 33.593212980717055 32.56665822510039 18.276292464093196 20-21 19.809341196049346 27.50804963640968 29.805361600520964 22.877247567020007 22-23 17.057089106761694 29.242791505372455 31.912738323504936 21.787381064360915 24-25 22.048768134293258 30.01790817987772 30.196989978654898 17.736333707174126 26-27 20.3449616960466 26.797391532429476 36.91922252471442 15.93842424680951 28-29 15.972649325277668 34.030968488839044 33.39513765782714 16.601244528056146 30-31 18.221120798813356 30.30552440215622 31.900075974096453 19.573278824933976 32-33 17.31033609493144 31.42523787127817 34.113273759994215 17.15115227379617 34-35 19.501275302545178 28.038566596118013 33.20399413903511 19.256163962301695 36-37 17.560269573476862 28.59378533628839 37.20566285223212 16.640282238002623 38-39 18.219396154054884 28.8255938059661 35.632880479024585 17.32212956095443 40-41 16.27484147587991 30.6953351846659 35.04445911841593 17.985364221038253 42-43 16.009551461210755 31.66725458804801 31.43841749654031 20.884776454200924 44-45 17.809594443037515 28.31482218443616 32.26095293223834 21.61463044028798 46-47 20.665858688180602 30.634745486776886 31.37458847364422 17.324807351398285 48-49 19.2684779856011 28.524655403205383 32.79186715386563 19.414999457327884 50-51 14.521905864476684 30.361600520965233 36.55168047465721 18.56481313990087 52-53 17.208132846134365 32.359538366918706 30.79845157555805 19.633877211388878 54-55 14.679280778553597 28.414312072645707 32.450888173365655 24.45551897543504 56-57 19.973951738359684 28.303064288556858 29.042002821895014 22.68098115118845 58-59 21.956712462577897 28.094389624015264 30.086918770293856 19.861979143112976 60-61 17.741016434069266 30.87651384278647 32.742418349719166 18.640051373425106 62-63 16.110563399390383 31.038069482005408 31.704669820280206 21.146697298324003 64-65 15.243659780760465 34.915524040374805 29.688687095257045 20.152129083607683 66-67 17.27596686082269 30.12101588220397 27.97655656452371 24.626460692449623 68-69 15.567454144206069 30.435765710357803 29.83701747404218 24.159762671393946 70-71 19.954053760717773 29.911182663434754 27.59849498932745 22.536268586520023 72-73 18.279005824680727 25.999421149741327 32.89407040266271 22.827502622915237 74-75 15.833363481784307 32.56304041098368 28.61510075612315 22.98849535110886 76-77 17.682457110231013 26.846774997507726 29.80487760669198 25.665890285569283 78-79 17.676639774248397 27.596686082269095 31.657682428276836 23.068991715205673 80-81 19.2749834935738 27.121188824471115 29.847236417246275 23.75659126470881 82-83 16.756810535074706 26.66148113309938 32.49701530335371 24.0846930284722 84-85 16.913280995622447 28.347382511486558 30.166238558662855 24.573097934228137 86-87 17.16942186170309 28.824530590812795 29.021890673378113 24.984156874105995 88-89 17.01206787042918 28.209781326558385 26.98484711006261 27.793303692949824 90-91 18.06683422100841 25.593169589794307 28.734359830510016 27.60563635868727 92-93 19.36073224557722 28.809558264896353 28.55269346260989 23.277016026916534 94-95 18.06826815238233 27.944900691002495 28.379038385007778 25.607792771607397 96-97 14.265041062190226 28.766144495495823 30.273868528635 26.694945913678954 98-99 17.74266488187837 29.326001230056796 25.7696899533302 27.16164393473463 100-101 17.056184653232517 27.087478745342064 27.611157338735936 28.245179262689486 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 887.0 1 525.5 2 193.0 3 218.0 4 165.0 5 130.5 6 229.5 7 429.0 8 572.0 9 556.0 10 400.0 11 265.5 12 233.5 13 205.5 14 196.0 15 194.0 16 177.5 17 197.5 18 209.5 19 216.0 20 231.5 21 241.0 22 286.5 23 331.5 24 367.5 25 445.0 26 528.5 27 628.0 28 714.0 29 769.5 30 866.0 31 930.0 32 907.5 33 861.0 34 851.0 35 807.0 36 688.0 37 575.5 38 498.5 39 457.5 40 463.5 41 488.0 42 519.5 43 588.5 44 657.5 45 741.5 46 1313.0 47 3708.0 48 8413.5 49 8506.5 50 4887.5 51 3019.5 52 1420.5 53 621.0 54 367.5 55 275.0 56 234.5 57 194.5 58 178.5 59 152.5 60 110.0 61 92.5 62 83.0 63 76.0 64 68.0 65 55.5 66 53.5 67 46.5 68 38.0 69 25.0 70 28.0 71 30.5 72 20.0 73 17.0 74 8.0 75 6.5 76 8.5 77 5.5 78 5.0 79 4.0 80 2.5 81 2.0 82 1.0 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 1.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26229152346152457 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0018089070583553416 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 9.044535291776708E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0018089070583553416 36-37 0.017184617054375746 38-39 0.005426721175066026 40-41 0.011757895879309721 42-43 0.004522267645888355 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 9.044535291776708E-4 60-61 9.044535291776708E-4 62-63 0.002713360587533013 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.2016931370066206 78-79 0.0 80-81 9.044535291776708E-4 82-83 0.0 84-85 0.0 86-87 0.0958720740928331 88-89 0.3201765493288955 90-91 0.3174631887413625 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 55282.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.72493759270649 #Duplication Level Percentage of deduplicated Percentage of total 1 81.55989165883588 26.690423646033068 2 8.899452766569011 5.8246807279042 3 3.504505002487425 3.4405412249918594 4 1.8241114366259465 2.387757317029051 5 1.0004974849372616 1.6370608878115842 6 0.6854236913382346 1.3458268514163743 7 0.46431927477751367 1.0636373503129408 8 0.2376872478027749 0.6222640280742375 9 0.2542700790448289 0.7488875221591115 >10 1.1110496932176221 6.877464635867009 >50 0.13266264993643248 3.1746318874136246 >100 0.23215963738875683 17.72367135776564 >500 0.06633132496821624 14.92710104554828 >1k 0.027638052070090104 13.536051517673023 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2578 4.663362396440071 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1494 2.7025071451828806 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1224 2.2141022394269383 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1145 2.0711985818168666 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1042 1.884881154806266 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 928 1.6786657501537572 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 776 1.4037118772837451 No Hit CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT 737 1.3331645020078868 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 737 1.3331645020078868 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 713 1.2897507326073585 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 713 1.2897507326073585 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 712 1.2879418255490034 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 674 1.2192033573315002 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 594 1.0744907926630731 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 590 1.0672551644296515 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 545 0.9858543468036612 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 533 0.9641474621033972 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 494 0.8936000868275389 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 491 0.8881733656524728 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 477 0.862848666835498 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 440 0.7959191056763503 No Hit ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT 421 0.7615498715675988 RNA PCR Primer, Index 33 (95% over 22bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 411 0.7434608009840454 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 411 0.7434608009840454 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 406 0.7344162656922688 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 350 0.6331174704243696 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 310 0.560761188090156 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 293 0.5300097680981151 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 266 0.48116927752252087 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 260 0.47031583517238884 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 247 0.44680004341376944 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 241 0.4359466010636374 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 240 0.434137694005282 No Hit ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT 233 0.42147534459679464 RNA PCR Primer, Index 33 (95% over 24bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 225 0.4070040881299519 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 219 0.39615064577981984 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 217 0.39253283166310915 No Hit TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG 211 0.38167938931297707 RNA PCR Primer, Index 33 (96% over 29bp) TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 207 0.37444376107955574 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 195 0.35273687637929163 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 186 0.33645671285409356 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 180 0.3256032705039615 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 166 0.30027857168698674 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 165 0.2984696646286314 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 146 0.26410043051987986 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 140 0.25324698816974783 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 134 0.2423935458196158 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 132 0.2387757317029051 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 130 0.23515791758619442 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 128 0.23154010346948373 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 126 0.22792228935277306 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 122 0.22068666111935167 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 119 0.21525993994428566 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 113 0.20440649759415364 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 112 0.2025975905357983 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 111 0.20078868347744291 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 109 0.19717086936073225 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 107 0.19355305524402158 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 107 0.19355305524402158 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 100 0.18089070583553415 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 99 0.17908179877717884 No Hit CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCT 98 0.1772728917188235 RNA PCR Primer, Index 33 (96% over 27bp) TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC 95 0.17184617054375745 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 94 0.17003726348540213 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 94 0.17003726348540213 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 83 0.15013928584349337 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 83 0.15013928584349337 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 79 0.14290365761007198 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 77 0.13928584349336132 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 76 0.13747693643500597 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 73 0.13205021525993993 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 69 0.12481458702651858 No Hit CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTG 68 0.12300567996816324 RNA PCR Primer, Index 15 (95% over 24bp) ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 61 0.11034333055967584 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 61 0.11034333055967584 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 60 0.1085344235013205 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 58 0.10491660938460982 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 57 0.10310770232625446 No Hit TATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCT 57 0.10310770232625446 RNA PCR Primer, Index 33 (95% over 21bp) CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 56 0.10129879526789914 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0018089070583553416 0.0 18-19 0.0 0.0 0.0 0.0036178141167106832 0.0 20-21 0.0 0.0 0.0 0.0036178141167106832 0.0 22-23 0.0 0.0 0.0 0.0072356282334213665 0.0 24-25 0.0 0.0 0.0 0.017184617054375746 0.0 26-27 0.0 0.0 0.0 0.05969393292572628 0.0 28-29 0.0 0.0 0.0 0.15556600701855938 0.0 30-31 0.0 0.0 0.0 0.36630367931695673 0.0 32-33 0.0 0.0 0.0 0.6692956115914764 0.0 34-35 0.0 0.0 0.0 1.1522737961723526 0.0 36-37 0.0 0.0 0.0 1.7184617054375746 0.0 38-39 0.0 0.0 0.0 2.310878767048949 0.0 40-41 0.0 0.0 0.0 2.9458051445316737 0.0 42-43 0.0 0.0 0.0 3.4957128902716974 0.0 44-45 0.0 0.0 0.0 4.042907275424189 0.0 46-47 0.0 0.0 0.0 4.650700047031584 0.0 48-49 0.0 0.0 0.0 5.219601316884338 0.0 50-51 0.0 0.0 0.0 5.713432943815347 0.0 52-53 0.0 0.0 0.0 6.091494519011613 0.0 54-55 0.0 0.0 0.0 6.5988929488802865 0.0 56-57 0.0 0.0 0.0 7.260048478709164 0.0 58-59 0.0 0.0 0.0 7.763829094461126 0.0 60-61 0.0 0.0 0.0 8.333634817843059 0.0 62-63 0.0 0.0 0.0 8.832893165949134 0.0 64-65 0.0 0.0 0.0 9.34300495640534 0.0 66-67 0.0 0.0 0.0 9.967077891537933 0.0 68-69 0.0 0.0 0.0 10.710538692521979 0.0 70-71 0.0 0.0 0.0 11.468470749972866 0.0 72-73 0.0 0.0 0.0 12.136861908035165 0.0 74-75 0.0 0.0 0.0 12.970768061936976 0.0 76-77 0.0 0.0 0.0 13.601172171773815 0.0 78-79 0.0 0.0 0.0 14.513765782714085 0.0 80-81 0.0 0.0 0.0 15.419123765420933 0.0 82-83 0.0 0.0 0.0 16.205998335805507 0.0 84-85 0.0 0.0 0.0 17.10231178322058 0.0 86-87 0.0 0.0 0.0 17.9398357512391 0.0 88-89 0.0 0.0 0.0 18.900365399225787 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCTTGA 90 8.88266E-4 26.367018 8 AGTACTC 110 9.3702314E-5 25.887619 7 GAGTAAA 50 0.001652191 23.730318 10-11 GGGCTTG 100 0.0016417768 23.730318 7 GCTTGAT 105 0.00218054 22.600302 9 CCCCAGA 55 0.0028523179 21.602339 80-81 TGGGCTT 110 0.0028569277 21.573015 6 AGTACTT 745 0.0 21.34136 12-13 TACTTTT 795 0.0 19.999136 14-15 CATGGGG 120 0.004730015 19.775265 6 GCAGAGA 130 6.5663116E-6 16.42868 94-95 GAGTACT 825 0.0 16.395493 12-13 GTACTTT 790 0.0 16.220722 14-15 ACTTTTT 845 0.0 15.445768 16-17 AGTACAT 670 1.0913936E-11 12.750619 2 AGAGTAC 2055 0.0 12.355932 10-11 CTTTTTT 1300 0.0 12.230241 16-17 CTTGAAA 265 3.0093724E-7 11.641288 54-55 GTACATG 740 1.2369128E-10 11.544478 3 CATGGGA 435 2.3250323E-7 10.91049 6 >>END_MODULE