FastQCFastQC Report
Fri 27 May 2016
SRR939275_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939275_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2484815
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53900.21691755724269213No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42520.17111937910870628No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28480.11461617866923694No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA110050.028.0703981
GGTATCA78750.026.4527951
GTACATG90250.023.7656041
ATCAACG129750.023.312793
TATCAAC131450.023.22772
TCAACGC130500.023.1788084
CAACGCA132150.022.8893995
TACATGG91700.022.4388872
AACGCAG136150.022.3092446
GTATAGA7600.021.228681
GAGTACT61300.021.10184712-13
ACGCAGA148750.020.1009647
CGCAGAG148850.019.9605278
ACATGGG95300.019.9495183
GTACTTT64550.019.897214-15
AGTACTT64950.018.74874712-13
GCAGAGT157750.018.4130979
AGAGTAC120750.018.38347410-11
TAAGGCG4650.018.352725
ATCGCGA650.007531566518.28392656-57
Produced by FastQC (version 0.11.5)