##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939275_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2484815 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.89515275785119 33.0 31.0 34.0 30.0 34.0 2 31.962871280155664 33.0 31.0 34.0 30.0 34.0 3 32.016442672794554 34.0 31.0 34.0 30.0 34.0 4 35.4899656513664 37.0 35.0 37.0 33.0 37.0 5 35.525449580753495 37.0 35.0 37.0 33.0 37.0 6 35.613456937437995 37.0 35.0 37.0 33.0 37.0 7 35.551300197399 37.0 35.0 37.0 33.0 37.0 8 35.53352181148295 37.0 35.0 37.0 33.0 37.0 9 37.16620472751492 39.0 37.0 39.0 34.0 39.0 10-11 37.0628529689333 39.0 37.0 39.0 33.0 39.0 12-13 36.9655869752879 39.0 37.0 39.0 33.0 39.0 14-15 38.20637109804955 40.0 38.0 41.0 33.0 41.0 16-17 38.12797874288428 40.0 38.0 41.0 33.0 41.0 18-19 38.04221783110614 40.0 38.0 41.0 33.0 41.0 20-21 38.01315892732457 40.0 38.0 41.0 33.0 41.0 22-23 38.0222342508396 40.0 38.0 41.0 33.0 41.0 24-25 37.948413262154325 40.0 38.0 41.0 33.0 41.0 26-27 37.866431303738906 40.0 38.0 41.0 33.0 41.0 28-29 37.72449940941277 40.0 38.0 41.0 32.0 41.0 30-31 37.5613768026996 40.0 37.5 41.0 32.0 41.0 32-33 37.355323032096955 40.0 37.0 41.0 31.5 41.0 34-35 37.08314783998004 40.0 37.0 41.0 30.5 41.0 36-37 36.8558596917678 40.0 36.5 41.0 30.0 41.0 38-39 36.28342089853772 39.5 35.5 40.5 29.5 41.0 40-41 36.276322985815845 39.0 35.5 40.0 30.0 41.0 42-43 36.475875668812364 39.5 36.0 41.0 30.0 41.0 44-45 36.39793787465062 40.0 36.0 41.0 30.0 41.0 46-47 36.159826586687544 40.0 36.0 41.0 29.0 41.0 48-49 35.94173389165793 39.0 35.5 41.0 28.0 41.0 50-51 34.825534898976386 38.0 34.0 40.0 25.5 40.5 52-53 34.93092684968499 38.5 34.5 39.5 26.5 40.5 54-55 35.4280338777736 39.0 35.0 40.5 27.0 41.0 56-57 35.34196610210418 39.0 35.0 41.0 26.5 41.0 58-59 35.13199413235995 39.0 35.0 40.5 26.0 41.0 60-61 34.82259785939799 38.0 34.5 40.0 26.0 41.0 62-63 34.438068025184975 38.0 34.0 40.0 25.5 41.0 64-65 34.04079197042839 37.0 34.0 40.0 24.0 41.0 66-67 33.67626402770428 37.0 33.5 39.5 23.0 41.0 68-69 33.265222360618395 36.0 33.0 39.0 22.0 41.0 70-71 32.85046874717031 35.5 33.0 39.0 22.0 40.0 72-73 32.40345538802687 35.0 32.0 38.0 20.5 40.0 74-75 31.9367655137304 35.0 32.0 37.0 19.5 39.5 76-77 31.47251022711952 35.0 31.0 37.0 18.0 39.0 78-79 31.076935506265052 35.0 31.0 36.0 16.0 39.0 80-81 30.66945507009576 35.0 31.0 36.0 10.0 37.5 82-83 30.2418673019923 34.0 30.5 35.0 7.0 37.0 84-85 29.82678388531943 34.0 30.0 35.0 4.0 36.5 86-87 29.40210438201637 34.0 29.5 35.0 2.0 36.0 88-89 28.98256992975332 34.0 29.0 35.0 2.0 36.0 90-91 28.57223415022849 34.0 29.0 35.0 2.0 35.5 92-93 28.121577461501158 34.0 29.0 35.0 2.0 35.0 94-95 27.64609900535855 34.0 27.0 35.0 2.0 35.0 96-97 27.038631447411575 33.5 26.5 35.0 2.0 35.0 98-99 26.231904990914817 33.0 25.0 35.0 2.0 35.0 100-101 23.425652412755074 30.0 11.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.36691502666532827 1101 2 0.35514555114406576 1101 3 0.40371394903168323 1101 4 0.3390474020234038 1101 5 0.3409403727377125 1101 6 0.4061609134218571 1101 7 0.3760767339252524 1101 8 0.25185767445703533 1101 9 0.4579273253809646 1101 10-11 0.49253093785227975 1101 12-13 0.5800754879062282 1101 14-15 0.7058218878524727 1101 16-17 0.6646673190394807 1101 18-19 0.5907545823587625 1101 20-21 0.5909924910680715 1101 22-23 0.7263575320187954 1101 24-25 0.8057518167721156 1101 26-27 0.6985394718988047 1101 28-29 0.6740906750557443 1101 30-31 0.7350753618277395 1101 32-33 0.6236311907239624 1101 34-35 0.7167747101874369 1101 36-37 0.5297420457510214 1101 38-39 0.9090476689616338 1101 40-41 0.8355257677243699 1101 42-43 0.7652592105634781 1101 44-45 0.6242023715837419 1101 46-47 0.7099225013874175 1101 48-49 0.7706555587337434 1101 50-51 0.713172513303796 1101 52-53 0.8134670151251129 1101 54-55 0.7777198649699031 1101 56-57 0.7299017569760267 1101 58-59 0.7013170051220641 1101 60-61 0.6407762803487174 1101 62-63 0.7106314047310818 1101 64-65 0.5853516961160281 1101 66-67 0.4669742163016011 1101 68-69 0.6972998917891857 1101 70-71 0.7269350271980812 1101 72-73 0.6898993666535276 1101 74-75 0.4439746354853149 1101 76-77 0.5963716298811441 1101 78-79 0.6531511262503535 1101 80-81 0.5362397506612879 1101 82-83 0.4163708901117218 1101 84-85 0.4777468024465712 1101 86-87 0.45554179221975843 1101 88-89 0.41267359492432476 1101 90-91 0.35803909602746486 1101 92-93 0.4028195192026658 1101 94-95 0.5411854138565921 1101 96-97 0.5152004593601625 1101 98-99 0.520883986884126 1101 100-101 0.3061619176732009 1102 1 0.2836371991965869 1102 2 0.32142811722776443 1102 3 0.4000370819729895 1102 4 0.31292475626500504 1102 5 0.2083780951576415 1102 6 0.31353351953321607 1102 7 0.30423232162134894 1102 8 0.2871570255141833 1102 9 0.37930643995141367 1102 10-11 0.4336213743503947 1102 12-13 0.39860659656450537 1102 14-15 0.5054219284510495 1102 16-17 0.5473830306881027 1102 18-19 0.41279405812965564 1102 20-21 0.5631610576211941 1102 22-23 0.5866960543575246 1102 24-25 0.5997061180008529 1102 26-27 0.517354453407421 1102 28-29 0.49348846814256575 1102 30-31 0.5603119753990811 1102 32-33 0.4859568230150515 1102 34-35 0.506157706996639 1102 36-37 0.4707757869731637 1102 38-39 0.8949606769988776 1102 40-41 0.6287902103503384 1102 42-43 0.4440436004112911 1102 44-45 0.4108972986648993 1102 46-47 0.5070461874843133 1102 48-49 0.41396337522817817 1102 50-51 0.36863809649599233 1102 52-53 0.33359700985998586 1102 54-55 0.35528217426762154 1102 56-57 0.3435393100237647 1102 58-59 0.39028791053372913 1102 60-61 0.42490762942668425 1102 62-63 0.5311682574534089 1102 64-65 0.5312873981224868 1102 66-67 0.4893720220754787 1102 68-69 0.47872731232064325 1102 70-71 0.4417157631741304 1102 72-73 0.3231582428342179 1102 74-75 0.30568772091359264 1102 76-77 0.2255543932485402 1102 78-79 0.4013860397563569 1102 80-81 0.3240895961963979 1102 82-83 0.33315332615310567 1102 84-85 0.3488859730938003 1102 86-87 0.17414489991381998 1102 88-89 0.20199553528247804 1102 90-91 0.20581109062277747 1102 92-93 -0.035772756918397874 1102 94-95 0.08480786930917006 1102 96-97 0.04610136711893276 1102 98-99 -0.13436750225892524 1102 100-101 -0.3036810165888255 1103 1 0.31068663089497406 1103 2 0.42743601403261877 1103 3 0.4583718528314762 1103 4 0.3796043606580781 1103 5 0.3781809625411512 1103 6 0.3835697451671649 1103 7 0.38822931240201797 1103 8 0.3252658174180425 1103 9 0.5055522950872131 1103 10-11 0.5354197183214211 1103 12-13 0.578343960718378 1103 14-15 0.5562743808689703 1103 16-17 0.5986978870314204 1103 18-19 0.6913936211220104 1103 20-21 0.6280377658802578 1103 22-23 0.5916794749007224 1103 24-25 0.6615017306740754 1103 26-27 0.5167219423384779 1103 28-29 0.5775022300500652 1103 30-31 0.42428855332253335 1103 32-33 0.4604299700451264 1103 34-35 0.4878328550617326 1103 36-37 0.3945354423504668 1103 38-39 0.87524621741251 1103 40-41 0.657587433051738 1103 42-43 0.678462025650397 1103 44-45 0.5457407521753339 1103 46-47 0.5676654254043072 1103 48-49 0.6319149497973982 1103 50-51 0.5012366148867287 1103 52-53 0.5857281409047772 1103 54-55 0.7466711589915178 1103 56-57 0.6994405903795098 1103 58-59 0.7040960584263161 1103 60-61 0.7764675838092217 1103 62-63 0.6847079235642326 1103 64-65 0.5749210783785585 1103 66-67 0.6822711269764739 1103 68-69 0.7157105220268178 1103 70-71 0.5846285245641241 1103 72-73 0.6989189332296277 1103 74-75 0.5327903410427801 1103 76-77 0.44674998292135726 1103 78-79 0.5945295239339963 1103 80-81 0.4859441474010424 1103 82-83 0.4840407583498383 1103 84-85 0.4763333596730668 1103 86-87 0.3321912073908706 1103 88-89 0.40929265256536596 1103 90-91 0.4185684076258269 1103 92-93 0.48915273136098136 1103 94-95 0.3017388010171409 1103 96-97 0.39193278375205765 1103 98-99 0.34949627785722726 1103 100-101 0.4224476802467052 1104 1 0.24983873662974432 1104 2 0.350487089301442 1104 3 0.24768636896050822 1104 4 0.08412945026381635 1104 5 0.2122843451576415 1104 6 0.1730802738409949 1104 7 0.30969828641635644 1104 8 0.3430189536187598 1104 9 0.3329072458040585 1104 10-11 0.409719673518552 1104 12-13 0.4249221417219644 1104 14-15 0.4489925148295413 1104 16-17 0.48176940360848164 1104 18-19 0.4839003415521219 1104 20-21 0.5062929742135864 1104 22-23 0.46099511110143965 1104 24-25 0.523907924670489 1104 26-27 0.5371282483420643 1104 28-29 0.617145767236444 1104 30-31 0.5911372316379442 1104 32-33 0.5102101559770276 1104 34-35 0.502687805778578 1104 36-37 0.4338149562393596 1104 38-39 0.7506522433981644 1104 40-41 0.5919512323348926 1104 42-43 0.5574942837149166 1104 44-45 0.4990687651681682 1104 46-47 0.5510953649204353 1104 48-49 0.5179826581307339 1104 50-51 0.5447233980408512 1104 52-53 0.5192378855284403 1104 54-55 0.565177543400118 1104 56-57 0.5235053305228732 1104 58-59 0.4473196247345612 1104 60-61 0.5631849337196186 1104 62-63 0.5190143191735572 1104 64-65 0.4837822454932521 1104 66-67 0.5337496216001298 1104 68-69 0.5040169736397218 1104 70-71 0.541799783524695 1104 72-73 0.5097948292869816 1104 74-75 0.293333015773964 1104 76-77 0.42019728354859964 1104 78-79 0.46063895493758267 1104 80-81 0.43116564157666915 1104 82-83 0.2934815625613041 1104 84-85 0.42427102768381886 1104 86-87 0.3526185147385412 1104 88-89 0.30011366511016746 1104 90-91 0.30026783638927057 1104 92-93 0.19852913145059148 1104 94-95 0.15253825218201555 1104 96-97 0.2451123872466816 1104 98-99 0.3940961322479062 1104 100-101 0.04263137200297962 1105 1 0.17961912410320835 1105 2 0.2919635578081632 1105 3 0.38554464921528364 1105 4 0.2853591034839127 1105 5 0.29319762806634486 1105 6 0.30303758983533413 1105 7 0.21423152686221414 1105 8 0.23533362099313848 1105 9 0.37390434859953103 1105 10-11 0.3720593320465042 1105 12-13 0.2689961420758209 1105 14-15 0.39893584241269053 1105 16-17 0.37160955974790255 1105 18-19 0.4159929636327959 1105 20-21 0.5264011770621337 1105 22-23 0.4981556295020013 1105 24-25 0.4981850057922159 1105 26-27 0.4311267776237315 1105 28-29 0.48417928413311984 1105 30-31 0.4664540442706411 1105 32-33 0.4889939382664821 1105 34-35 0.36760564459669354 1105 36-37 0.26926120314310253 1105 38-39 0.8029860411688716 1105 40-41 0.7191097602895979 1105 42-43 0.46212096453586327 1105 44-45 0.4058642026239383 1105 46-47 0.3913689959360127 1105 48-49 0.4165310149070933 1105 50-51 0.3750588987485628 1105 52-53 0.35960578627152984 1105 54-55 0.4821433298473963 1105 56-57 0.526527490820115 1105 58-59 0.548458602087706 1105 60-61 0.4471764964407754 1105 62-63 0.61275851370646 1105 64-65 0.636229664548452 1105 66-67 0.5033444552873263 1105 68-69 0.4110498823446065 1105 70-71 0.3923200639564044 1105 72-73 0.43034286538040334 1105 74-75 0.3539315870761577 1105 76-77 0.47606218756487806 1105 78-79 0.6061035028852082 1105 80-81 0.6120305455929476 1105 82-83 0.49627565093710047 1105 84-85 0.4908058273122684 1105 86-87 0.5042302229834092 1105 88-89 0.4759419117485244 1105 90-91 0.494839724658906 1105 92-93 0.4951777182340287 1105 94-95 0.6690940620723218 1105 96-97 0.7225725230393216 1105 98-99 0.7455787379626955 1105 100-101 0.44901406839363744 1106 1 0.15676004733958493 1106 2 0.1841793198258621 1106 3 0.18935044118964584 1106 4 0.1650845491983688 1106 5 0.20308544260471706 1106 6 0.16464943289731337 1106 7 0.2610120447185551 1106 8 0.18117577871907997 1106 9 0.21980652541524393 1106 10-11 0.3317362865668372 1106 12-13 0.39517583447137383 1106 14-15 0.488906044388834 1106 16-17 0.38887205469122677 1106 18-19 0.44321307505149576 1106 20-21 0.5102589503274686 1106 22-23 0.5043730255195911 1106 24-25 0.42608330648521076 1106 26-27 0.4044597936755352 1106 28-29 0.44720065040027634 1106 30-31 0.4154111134353684 1106 32-33 0.39807559331271136 1106 34-35 0.2685133341790191 1106 36-37 0.32400998227021205 1106 38-39 0.7584566357809024 1106 40-41 0.7196840398622228 1106 42-43 0.5788810970537881 1106 44-45 0.48728974854646623 1106 46-47 0.44684302776463625 1106 48-49 0.42940180464727007 1106 50-51 0.2546079888115713 1106 52-53 0.3061356517177245 1106 54-55 0.38284425763146857 1106 56-57 0.5546288516934865 1106 58-59 0.5552330915949426 1106 60-61 0.547520746902812 1106 62-63 0.5069850474321669 1106 64-65 0.6200721025397442 1106 66-67 0.5049081244367173 1106 68-69 0.528793852012484 1106 70-71 0.46444608818084987 1106 72-73 0.551480954235096 1106 74-75 0.600351725992585 1106 76-77 0.6557151873295588 1106 78-79 0.8315132587635112 1106 80-81 0.7786075802268719 1106 82-83 0.6593840052242399 1106 84-85 0.671069329624455 1106 86-87 0.8821913189795794 1106 88-89 0.6712987609313927 1106 90-91 0.694905296175488 1106 92-93 0.6819677016560703 1106 94-95 0.5624499223944142 1106 96-97 0.6361008787542204 1106 98-99 0.8146136468632363 1106 100-101 0.7417155355563168 1107 1 0.17844344049759187 1107 2 0.18420373806140233 1107 3 0.23315675574536243 1107 4 0.22390807861012973 1107 5 0.1517705206209783 1107 6 0.14329568591907105 1107 7 0.23578800740749273 1107 8 0.21201601020395344 1107 9 0.33197169036041885 1107 10-11 0.29633594959518916 1107 12-13 0.33748775301214096 1107 14-15 0.3839259452507946 1107 16-17 0.37906202856371607 1107 18-19 0.43889715192009504 1107 20-21 0.29366920110274464 1107 22-23 0.25744972319741066 1107 24-25 0.2923324213241756 1107 26-27 0.39752501478265856 1107 28-29 0.3547410015344994 1107 30-31 0.33343909673329364 1107 32-33 0.46350425543758433 1107 34-35 0.5086910989337383 1107 36-37 0.41138453358513516 1107 38-39 0.7750854541824808 1107 40-41 0.5417117301413228 1107 42-43 0.5024703334955589 1107 44-45 0.5369381259547126 1107 46-47 0.5567739241580583 1107 48-49 0.5054096673191069 1107 50-51 0.4851954915586205 1107 52-53 0.6397986312308248 1107 54-55 0.5986648324367323 1107 56-57 0.42373490009043024 1107 58-59 0.5209315752225194 1107 60-61 0.563697827946946 1107 62-63 0.6242475190459658 1107 64-65 0.6090167463997815 1107 66-67 0.4945731062451344 1107 68-69 0.4350705594576141 1107 70-71 0.5202966974158301 1107 72-73 0.45332575193001645 1107 74-75 0.576922428993587 1107 76-77 0.5226052808513977 1107 78-79 0.6181833439831514 1107 80-81 0.5071500557475908 1107 82-83 0.4290834167447599 1107 84-85 0.48452621922961114 1107 86-87 0.6737511558657658 1107 88-89 0.6067918871980886 1107 90-91 0.5696946888939678 1107 92-93 0.5730501620374824 1107 94-95 0.5631458421072573 1107 96-97 0.7934092566508362 1107 98-99 1.1029685903350241 1107 100-101 0.8902760805713328 1108 1 0.03471919634255016 1108 2 0.029800290116934747 1108 3 0.03504426684642681 1108 4 0.03633272553749123 1108 5 0.1608112822338299 1108 6 0.07096653951931842 1108 7 0.11328106713035879 1108 8 0.023712317754736034 1108 9 0.11460304617413186 1108 10-11 0.2799763575809493 1108 12-13 0.355154643494771 1108 14-15 0.26497174866923245 1108 16-17 0.2838993944951085 1108 18-19 0.28400795689582026 1108 20-21 0.24840351954776452 1108 22-23 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-0.7804138522466388 2316 80-81 -0.6245594251533326 2316 82-83 -0.7292760548382589 2316 84-85 -0.622125976252125 2316 86-87 -0.6275442951601207 2316 88-89 -0.7714463466524073 2316 90-91 -0.624378862172076 2316 92-93 -0.8800568993583155 2316 94-95 -0.7619567204473725 2316 96-97 -0.6908928323968233 2316 98-99 -0.8774117113550624 2316 100-101 -0.03864577240527467 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9638.0 3 2211.0 4 2187.0 5 2464.0 6 3158.0 7 3610.0 8 4489.0 9 5551.0 10 6211.0 11 7693.0 12 9409.0 13 12286.0 14 12412.0 15 12524.0 16 15245.0 17 18760.0 18 17119.0 19 13465.0 20 13360.0 21 14856.0 22 15789.0 23 17946.0 24 20290.0 25 22969.0 26 27173.0 27 31491.0 28 37424.0 29 44787.0 30 53578.0 31 65928.0 32 82228.0 33 106386.0 34 144728.0 35 209975.0 36 331673.0 37 532391.0 38 487090.0 39 66308.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.71227475613802 18.93848696187091 15.051307284253898 23.29793099773717 2 14.990297196488525 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23.267740437409596 26.42729351399901 28.405460928064297 21.899505120527095 22-23 22.806185799751667 26.274289261928953 28.352925999387224 22.56659893893216 24-25 22.629880878597465 26.545330127299128 28.401125526078197 22.42366346802521 26-27 22.535868809737675 26.66164871546362 28.40775866100528 22.394723813793426 28-29 22.321301509168496 26.91528539551422 28.486437237578638 22.276975857738645 30-31 22.526726570086478 26.563515939197934 28.35225419839236 22.557503292323233 32-33 22.453131179482096 26.565347853062725 28.303615378754255 22.677905588700927 34-35 22.921250382936993 26.7319404692258 27.9896701420618 22.357139005775405 36-37 22.912795632442243 26.404581390486953 28.22266505618164 22.459957920889167 38-39 22.597766046272184 26.71503537810929 28.25232119891235 22.434877376706172 40-41 22.828693399363743 26.55266557976792 28.135540194538304 22.483100826330034 42-43 22.637164399174104 26.587517692991575 27.88965314257044 22.885664765263883 44-45 22.743209103356428 26.77655767256243 27.823870616511066 22.65636260757008 46-47 22.79523638358805 26.927334795109424 27.57051762318927 22.70691119811325 48-49 22.866471745337122 26.784362163048502 27.613145119206166 22.736020972408205 50-51 22.746804369985885 27.013682589320553 27.52966569260249 22.709847348091074 52-53 22.95694252762943 27.0191189071398 27.283797756325622 22.740140808905146 54-55 22.731316663031322 26.867666599080902 27.451579313580638 22.949437424307142 56-57 22.781892046125073 26.998370458744965 27.410140822756674 22.809596672373296 58-59 22.940371461240773 26.745403308078526 27.519595761459982 22.794629469220716 60-61 22.917630635392307 26.816187767340377 27.729802907063757 22.53637869020356 62-63 22.72019092237228 26.823752237624277 27.64943407868916 22.806622761314284 64-65 22.535699620779663 26.774205091948346 27.611615460026794 23.078479827245193 66-67 22.54668506092204 26.843791241242222 27.447158134049314 23.162365563786423 68-69 22.516208640213282 26.755064808272742 27.623259878969552 23.105466672544416 70-71 22.74092877444026 26.67937579162427 27.51115755486573 23.068537879069737 72-73 22.747092641215 26.63247402190664 27.532775645595603 23.087657691282757 74-75 22.558191447932714 27.000795445216795 27.279443908171576 23.161569198678922 76-77 22.713269184119024 26.71864639767122 27.33314465917361 23.234939759036145 78-79 22.7326235245893 26.794747824933967 27.373532472173466 23.099096178303267 80-81 22.62317493997567 26.97615418618043 27.274300127112728 23.12637074673118 82-83 22.75916090135891 26.826899266723604 27.338646344483248 23.07529348743424 84-85 22.730660530017744 26.755228688079036 27.246486732778223 23.267624049124997 86-87 22.689706880913004 26.725079844077893 27.356817872621026 23.228395402388074 88-89 22.762822513735088 26.690442611841693 27.24192836244778 23.304806511975443 90-91 22.683958140065304 26.75277760029246 27.405454020473808 23.15781023916843 92-93 22.818337996248637 26.566646526754944 27.365873445324183 23.249142031672235 94-95 22.64595027418755 26.53929212701597 27.39837266859703 23.416384930199452 96-97 22.667741028839952 26.48677503300934 27.411804908231 23.43367902991971 98-99 22.63581248903091 26.55676144768523 27.1470703129739 23.660355750309968 100-101 22.855821883814823 26.012555369186195 27.591548808881466 23.540073938117512 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2722.0 1 1551.0 2 379.5 3 349.5 4 313.5 5 352.0 6 497.0 7 854.5 8 1344.0 9 1649.5 10 1668.5 11 1706.5 12 1920.5 13 2078.5 14 2247.0 15 2447.0 16 2604.0 17 2708.5 18 2830.5 19 3018.0 20 3259.0 21 3630.5 22 4264.0 23 5264.0 24 6462.5 25 8076.0 26 10396.0 27 13509.0 28 16620.5 29 20365.5 30 24966.5 31 29716.5 32 35021.5 33 39732.0 34 44629.5 35 49681.5 36 54992.0 37 60869.5 38 65382.0 39 68776.5 40 72456.0 41 77886.0 42 82955.0 43 86619.5 44 90688.0 45 94574.5 46 96839.5 47 100484.0 48 107804.5 49 107485.5 50 99748.0 51 94492.5 52 91009.0 53 88430.5 54 85727.5 55 79729.5 56 70826.0 57 63396.5 58 57111.5 59 49634.0 60 41613.0 61 34128.0 62 27254.5 63 20943.5 64 15761.5 65 11584.5 66 8557.5 67 6974.5 68 5626.5 69 4232.0 70 3191.0 71 2464.0 72 1899.0 73 1503.0 74 1161.5 75 868.0 76 578.5 77 334.5 78 225.0 79 147.0 80 99.0 81 70.0 82 44.5 83 33.5 84 25.0 85 19.5 86 16.0 87 13.5 88 12.5 89 7.0 90 6.0 91 7.0 92 3.5 93 2.5 94 4.0 95 3.5 96 3.0 97 2.0 98 1.5 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2973662023128482 2 0.18528542366333106 3 0.18472200143672668 4 0.2054881349315744 5 0.21828586836444563 6 0.11284542309990885 7 0.1287017343343468 8 0.14339095667081855 9 0.14592635669053833 10-11 0.13254507880868394 12-13 0.12155834538989824 14-15 0.1381994232971066 16-17 0.11216126753903208 18-19 0.1414592233224606 20-21 0.16946935687365056 22-23 0.17284989023327693 24-25 0.19751973486959792 26-27 0.17655237915096295 28-29 0.1734535569046388 30-31 0.16274853459915525 32-33 0.174177956910273 34-35 0.3575920138923823 36-37 0.47705764815489277 38-39 0.4442785478999443 40-41 0.43478085893718443 42-43 0.45790129245034344 44-45 0.46222757026176997 46-47 0.4655477369542602 48-49 0.4769570370429992 50-51 0.4697331592090357 52-53 0.47560884814362436 54-55 0.45937021468399053 56-57 0.42252642550853886 58-59 0.4119622587597065 60-61 0.36845801397689565 62-63 0.4735966259057515 64-65 0.47144354811122763 66-67 0.4805790370711703 68-69 0.4915456482675773 70-71 0.4849858037721118 72-73 0.47754058149198236 74-75 0.48236991486287717 76-77 0.45939033690636927 78-79 0.446592603473498 80-81 0.4437553701180973 82-83 0.4558689479900918 84-85 0.457559214669905 86-87 0.45880679245738615 88-89 0.46631238140465187 90-91 0.4828126037552091 92-93 0.48748095934707414 94-95 0.4851467815511416 96-97 0.48414067043220527 98-99 0.4812028259649109 100-101 0.46403857027585554 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2484815.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.93748498925169 #Duplication Level Percentage of deduplicated Percentage of total 1 75.00881156032845 41.95804270717482 2 13.889328895110786 15.538682531620788 3 4.841280764770485 8.12427210324306 4 2.1388747052978245 4.7857308688594875 5 1.1289963570684682 3.157660838821864 6 0.688986965759309 2.3124118832970852 7 0.4611503735037582 1.8056914466958 8 0.2941846414961504 1.3164759174207443 9 0.23576122689789794 1.1869101081583855 >10 1.1980645551732534 12.144257270162123 >50 0.07796351563325432 2.9651988562530684 >100 0.03472357777204132 3.396697607850302 >500 0.0011525299620524906 0.4190122849201134 >1k 6.482981036501969E-4 0.6717735180450835 >5k 7.203312262779966E-5 0.21718205747738722 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5390 0.21691755724269213 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4252 0.17111937910870628 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2848 0.11461617866923694 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 4.024444475745679E-5 0.0 9 0.0 0.0 0.0 1.2073333427237037E-4 0.0 10-11 0.0 0.0 0.0 1.2073333427237037E-4 0.0 12-13 0.0 0.0 0.0 1.2073333427237037E-4 0.0 14-15 0.0 0.0 0.0 1.2073333427237037E-4 0.0 16-17 0.0 0.0 0.0 1.2073333427237037E-4 0.0 18-19 0.0 0.0 0.0 1.6097777902982717E-4 0.0 20-21 0.0 0.0 0.0 1.6097777902982717E-4 0.0 22-23 0.0 0.0 0.0 2.817111133021975E-4 0.0 24-25 0.0 0.0 0.0 4.628111147107531E-4 0.0 26-27 0.0 0.0 0.0 7.244000056342223E-4 0.0 28-29 0.0 0.0 0.0 0.0021329555721452103 0.0 30-31 0.0 0.0 0.0 0.006761066719252741 0.0 32-33 0.0 0.0 0.0 0.016600833462450927 0.0 34-35 0.0 0.0 0.0 0.03000223356668404 0.0 36-37 0.0 0.0 0.0 0.053706211528826084 0.0 38-39 0.0 0.0 0.0 0.08729020067892379 0.0 40-41 0.0 0.0 0.0 0.1245766787467075 0.0 42-43 0.0 0.0 0.0 0.16441867905658974 0.0 44-45 0.0 0.0 0.0 0.21587120167899826 0.0 46-47 0.0 0.0 0.0 0.27879339105728196 0.0 48-49 0.0 0.0 0.0 0.3460217360246135 0.0 50-51 0.0 0.0 0.0 0.4172342810229333 0.0 52-53 0.0 0.0 0.0 0.49498654829433986 0.0 54-55 0.0 0.0 0.0 0.5880719490183375 0.0 56-57 0.0 0.0 0.0 0.7000118721112034 0.0 58-59 0.0 0.0 0.0 0.8224555952857657 0.0 60-61 0.0 0.0 0.0 0.954236029644058 0.0 62-63 0.0 0.0 0.0 1.0947897529594757 0.0 64-65 0.0 0.0 0.0 1.2440966430096405 0.0 66-67 0.0 0.0 0.0 1.412982455434308 0.0 68-69 0.0 0.0 0.0 1.6102204791906036 0.0 70-71 0.0 0.0 0.0 1.831403947577586 0.0 72-73 0.0 0.0 0.0 2.0657071049555 0.0 74-75 0.0 0.0 0.0 2.309890273521369 0.0 76-77 0.0 0.0 0.0 2.5817213756356105 0.0 78-79 2.0122222378728396E-5 0.0 0.0 2.9006787225608344 0.0 80-81 8.048888951491358E-5 0.0 0.0 3.238410102965412 0.0 82-83 8.048888951491358E-5 0.0 0.0 3.5927624390548187 0.0 84-85 8.048888951491358E-5 0.0 0.0 3.9569545418874243 0.0 86-87 8.048888951491358E-5 0.0 0.0 4.346943333809559 0.0 88-89 8.048888951491358E-5 0.0 0.0 4.77560703714361 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 11005 0.0 28.070398 1 GGTATCA 7875 0.0 26.452795 1 GTACATG 9025 0.0 23.765604 1 ATCAACG 12975 0.0 23.31279 3 TATCAAC 13145 0.0 23.2277 2 TCAACGC 13050 0.0 23.178808 4 CAACGCA 13215 0.0 22.889399 5 TACATGG 9170 0.0 22.438887 2 AACGCAG 13615 0.0 22.309244 6 GTATAGA 760 0.0 21.22868 1 GAGTACT 6130 0.0 21.101847 12-13 ACGCAGA 14875 0.0 20.100964 7 CGCAGAG 14885 0.0 19.960527 8 ACATGGG 9530 0.0 19.949518 3 GTACTTT 6455 0.0 19.8972 14-15 AGTACTT 6495 0.0 18.748747 12-13 GCAGAGT 15775 0.0 18.413097 9 AGAGTAC 12075 0.0 18.383474 10-11 TAAGGCG 465 0.0 18.35272 5 ATCGCGA 65 0.0075315665 18.283926 56-57 >>END_MODULE