FastQCFastQC Report
Fri 27 May 2016
SRR939275_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939275_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2484815
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59180.2381666240746293No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46540.1872976459012039No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31190.12552242319850773No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT27210.10950513418503995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85600.026.7407111
GTATCAA113800.026.3032861
ATCAACG135700.021.9983983
TCAACGC135300.021.9582024
TATCAAC137250.021.7845422
CAACGCA137050.021.6778185
GAGTACT67950.021.33785412-13
GTACATG88050.021.1864571
AACGCAG140500.021.1455146
TACATGG88700.020.6530382
GTACTTT70950.020.00081814-15
ACATGGG91750.019.2423083
ACGCAGA153700.019.2059947
CGCAGAG155000.019.0142928
AGTACTT70700.018.72896612-13
GGACCGA3851.2732926E-1118.4905916
AGAGTAC126000.018.47536810-11
GCAGAGT167600.017.3299649
CCGTAAG3101.0945041E-716.8862271
ACTTTTT88750.016.12307716-17
Produced by FastQC (version 0.11.5)