##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939275_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2484815 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.32793628499506 33.0 31.0 34.0 31.0 34.0 2 32.53810645862972 34.0 31.0 34.0 31.0 34.0 3 32.63033746979151 34.0 31.0 34.0 31.0 34.0 4 36.14291607222268 37.0 35.0 37.0 35.0 37.0 5 36.08329714687009 37.0 35.0 37.0 35.0 37.0 6 36.11018164330141 37.0 35.0 37.0 35.0 37.0 7 36.0587130229011 37.0 35.0 37.0 35.0 37.0 8 36.030254566235314 37.0 35.0 37.0 35.0 37.0 9 37.74591468580156 39.0 38.0 39.0 35.0 39.0 10-11 37.66705147063262 39.0 37.0 39.0 35.0 39.0 12-13 37.595609532299186 39.0 37.0 39.0 35.0 39.0 14-15 38.90735628205722 40.0 38.0 41.0 35.0 41.0 16-17 38.90457961659118 40.0 38.0 41.0 35.0 41.0 18-19 39.000815352450786 40.0 38.5 41.0 35.0 41.0 20-21 38.98000474884448 40.0 38.5 41.0 35.0 41.0 22-23 38.9046331417027 40.0 38.0 41.0 35.0 41.0 24-25 38.84554363202089 40.0 38.0 41.0 35.0 41.0 26-27 38.69745192298018 40.0 38.0 41.0 34.0 41.0 28-29 38.53733859462375 40.0 38.0 41.0 34.0 41.0 30-31 38.36542519261998 40.0 38.0 41.0 34.0 41.0 32-33 38.33406169070937 40.0 38.0 41.0 34.0 41.0 34-35 38.25531840398581 40.0 38.0 41.0 34.0 41.0 36-37 38.12080859138406 40.0 38.0 41.0 33.0 41.0 38-39 37.99595261619074 40.0 38.0 41.0 33.0 41.0 40-41 37.826861154653365 40.0 37.0 41.0 33.0 41.0 42-43 37.66380032316289 40.0 37.0 41.0 33.0 41.0 44-45 37.43848817718825 40.0 37.0 41.0 32.0 41.0 46-47 37.16864997997839 40.0 37.0 41.0 31.5 41.0 48-49 36.886761589897034 40.0 36.0 41.0 31.0 41.0 50-51 36.651466004511406 39.0 36.0 41.0 30.5 41.0 52-53 36.402205999239385 39.0 35.5 41.0 30.0 41.0 54-55 36.160090389022926 39.0 35.0 40.0 30.0 41.0 56-57 35.8479037674837 38.5 35.0 40.0 29.0 41.0 58-59 35.53167076824633 38.0 35.0 40.0 28.0 41.0 60-61 35.192235438050716 38.0 34.0 40.0 28.0 41.0 62-63 34.83619967683711 37.5 34.0 40.0 27.0 41.0 64-65 34.51115736986456 37.0 34.0 40.0 26.5 41.0 66-67 34.08763670534829 36.5 33.5 39.0 26.0 40.5 68-69 33.61741799691325 36.0 33.0 39.0 26.0 40.0 70-71 33.13856705629996 35.0 32.5 38.5 25.0 40.0 72-73 32.63852158007739 35.0 32.0 37.5 24.5 39.5 74-75 32.111105856975264 35.0 31.5 37.0 22.5 39.0 76-77 30.149029002159114 33.0 29.5 35.0 20.0 37.5 78-79 31.117418198135475 34.0 31.0 36.0 20.0 38.0 80-81 31.126765976541513 34.0 31.0 35.5 20.5 37.0 82-83 30.770788770995026 34.0 31.0 35.0 19.5 37.0 84-85 30.322799282843995 34.0 30.5 35.0 18.0 36.5 86-87 29.71905795803712 34.0 29.5 35.0 10.5 36.0 88-89 29.133419590593263 34.0 29.0 35.0 6.5 35.5 90-91 28.645233347351812 33.5 29.0 35.0 2.0 35.0 92-93 28.271260435887584 33.0 29.0 35.0 2.0 35.0 94-95 27.744863702126718 33.0 27.0 35.0 2.0 35.0 96-97 27.00281912335526 33.0 26.5 34.5 2.0 35.0 98-99 25.973248310236375 32.0 24.5 34.0 2.0 35.0 100-101 21.567365980968404 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18128995104596157 1101 2 0.22825454356308938 1101 3 0.27150167363100053 1101 4 0.14852010606826127 1101 5 0.21238806293462886 1101 6 0.1787939692560201 1101 7 0.21471514484442622 1101 8 0.1875359167139834 1101 9 0.3291017737183779 1101 10-11 0.30209563168180864 1101 12-13 0.2554922514619662 1101 14-15 0.346765399860395 1101 16-17 0.2986069391642374 1101 18-19 0.3303303376190385 1101 20-21 0.3082530957503664 1101 22-23 0.3144818085603731 1101 24-25 0.3060650591249967 1101 26-27 0.2444946010622644 1101 28-29 0.28178097459114326 1101 30-31 0.38814941113655976 1101 32-33 0.3661386539406877 1101 34-35 0.3463488938858461 1101 36-37 0.32621059333928315 1101 38-39 0.3457492806916136 1101 40-41 0.37926202000340226 1101 42-43 0.3543620831954968 1101 44-45 0.4956249001420687 1101 46-47 0.4362749003110622 1101 48-49 0.3775669564343076 1101 50-51 0.4787013417103978 1101 52-53 0.43507435292098506 1101 54-55 0.43097697796498124 1101 56-57 0.48035880452665225 1101 58-59 0.4169117261828035 1101 60-61 0.4371031848452702 1101 62-63 0.3305930903427807 1101 64-65 0.36580385259444625 1101 66-67 0.39239527938899244 1101 68-69 0.3971988844747685 1101 70-71 0.2920485395937149 1101 72-73 0.45789401889432924 1101 74-75 0.4305324763656273 1101 76-77 0.5493397021453426 1101 78-79 0.789411053411829 1101 80-81 0.7193787452407605 1101 82-83 0.6989015608775375 1101 84-85 0.7369585990613601 1101 86-87 0.6665882254500701 1101 88-89 0.7013699795123394 1101 90-91 0.8470756355383635 1101 92-93 0.7876205737702087 1101 94-95 0.44740770085020287 1101 96-97 0.4649334536584888 1101 98-99 0.37889760527287564 1101 100-101 0.14532284285188624 1102 1 0.20406162846490616 1102 2 0.24168537691227954 1102 3 0.21521079752643857 1102 4 0.1722361761261766 1102 5 0.1906646534155314 1102 6 0.09867363254158334 1102 7 0.1488296835748315 1102 8 0.19597439401366756 1102 9 0.209393068495757 1102 10-11 0.2499343919028192 1102 12-13 0.23543243199875974 1102 14-15 0.2837424553218497 1102 16-17 0.190251958543719 1102 18-19 0.23246556893576553 1102 20-21 0.2337154596024078 1102 22-23 0.24829106503553078 1102 24-25 0.18872645432040258 1102 26-27 0.23285740008029165 1102 28-29 0.23894115182929454 1102 30-31 0.24582484138630178 1102 32-33 0.30905642279524415 1102 34-35 0.357290685678592 1102 36-37 0.3818968519902697 1102 38-39 0.35888632942893395 1102 40-41 0.3852475345586228 1102 42-43 0.31881612751137567 1102 44-45 0.2883489535292014 1102 46-47 0.36365856001887664 1102 48-49 0.42778350860029946 1102 50-51 0.35623518442897506 1102 52-53 0.3956291102493381 1102 54-55 0.2745555044904435 1102 56-57 0.23678638364629734 1102 58-59 0.2885425202021281 1102 60-61 0.2954549937021014 1102 62-63 0.2961826260600091 1102 64-65 0.3491497143943647 1102 66-67 0.2056925814867938 1102 68-69 0.17534828723167095 1102 70-71 0.02948478714352376 1102 72-73 0.07866724730572372 1102 74-75 0.07549436762488426 1102 76-77 0.12732028777978854 1102 78-79 0.036656376646266864 1102 80-81 0.19712247942159067 1102 82-83 0.18265380490034744 1102 84-85 0.1689405231800869 1102 86-87 0.1883017232076334 1102 88-89 0.24525846664449347 1102 90-91 0.4298035584771931 1102 92-93 0.3934111834466272 1102 94-95 0.25753403219801285 1102 96-97 0.07925809001578088 1102 98-99 -0.04220080821092509 1102 100-101 0.055553604519218425 1103 1 0.11023196561247062 1103 2 0.22133481244406994 1103 3 0.2597333475043939 1103 4 0.15966501863091764 1103 5 0.21956130494027093 1103 6 0.20157168325747676 1103 7 0.1961721787351678 1103 8 0.1995178935249058 1103 9 0.18998647888043507 1103 10-11 0.1964955921404865 1103 12-13 0.2400097516851858 1103 14-15 0.25348854046168157 1103 16-17 0.25801809236772755 1103 18-19 0.18654262986766668 1103 20-21 0.32458382063205704 1103 22-23 0.37782046721673623 1103 24-25 0.35255640467786975 1103 26-27 0.2272654272207646 1103 28-29 0.3070810421940422 1103 30-31 0.25709863242764186 1103 32-33 0.3782936240295953 1103 34-35 0.5200556694824812 1103 36-37 0.49056536827263386 1103 38-39 0.45346350536284774 1103 40-41 0.5090675659925097 1103 42-43 0.3731521825590107 1103 44-45 0.47142736573474764 1103 46-47 0.5532980766270867 1103 48-49 0.4339037719549097 1103 50-51 0.39323708196218377 1103 52-53 0.2426189325712329 1103 54-55 0.15586983374119967 1103 56-57 0.2190209685848714 1103 58-59 0.30165993725692175 1103 60-61 0.39591404340213643 1103 62-63 0.3687345207181707 1103 64-65 0.36692950470736463 1103 66-67 0.18301202832711283 1103 68-69 0.2561455866010718 1103 70-71 0.3185842734678701 1103 72-73 0.42812721702185996 1103 74-75 0.34190251935086735 1103 76-77 0.43238349096917617 1103 78-79 0.350241708139567 1103 80-81 0.5947048016955634 1103 82-83 0.5923424848689152 1103 84-85 0.40562784229150495 1103 86-87 0.3686045139231382 1103 88-89 0.44977636862444825 1103 90-91 0.5841123005468454 1103 92-93 0.582233706590781 1103 94-95 0.5238834849801073 1103 96-97 0.33060457191254855 1103 98-99 0.07142477398676306 1103 100-101 -0.013399685496690239 1104 1 0.09170878012860584 1104 2 0.11716814577740564 1104 3 0.09677691829292456 1104 4 0.07557563691049296 1104 5 0.1466166364814896 1104 6 0.1289070327198374 1104 7 0.12394155597530698 1104 8 0.1575432071449825 1104 9 0.22266457027828324 1104 10-11 0.16546260602937224 1104 12-13 0.14208048421206598 1104 14-15 0.2119688832036104 1104 16-17 0.22312085894478173 1104 18-19 0.15195341615798696 1104 20-21 0.19634961364280912 1104 22-23 0.2092076759981012 1104 24-25 0.2541161044986566 1104 26-27 0.3013931713963913 1104 28-29 0.34291633565283064 1104 30-31 0.25675561047423656 1104 32-33 0.15684845153855775 1104 34-35 0.24511307315631115 1104 36-37 0.3162542123586647 1104 38-39 0.4075055640546026 1104 40-41 0.580199118411862 1104 42-43 0.5499834997633073 1104 44-45 0.521354561075249 1104 46-47 0.44400708200343075 1104 48-49 0.303303413531971 1104 50-51 0.3916339793636183 1104 52-53 0.5056957694887956 1104 54-55 0.4820347082931775 1104 56-57 0.47778552951677256 1104 58-59 0.42856405912072404 1104 60-61 0.5987870272731115 1104 62-63 0.6763132057540133 1104 64-65 0.6388969106213409 1104 66-67 0.5868188719651073 1104 68-69 0.6620245628555566 1104 70-71 0.4674838030377586 1104 72-73 0.340180588437633 1104 74-75 0.31198960492434935 1104 76-77 0.3202433139978531 1104 78-79 0.5346545105589193 1104 80-81 0.6024963003514792 1104 82-83 0.6604638446359417 1104 84-85 0.5843352795674903 1104 86-87 0.5217743167905233 1104 88-89 0.6233314761513284 1104 90-91 0.6430910751884227 1104 92-93 0.64633680918935 1104 94-95 0.5485670647292089 1104 96-97 0.5013384988838752 1104 98-99 0.6437534030190228 1104 100-101 0.29080478358932993 1105 1 0.17824728419795832 1105 2 0.11192221513338296 1105 3 0.17658550374940063 1105 4 0.09688335984042595 1105 5 0.13828662055792051 1105 6 0.1544345008727035 1105 7 0.10904915063206744 1105 8 0.07329537451936119 1105 9 0.13759623163709733 1105 10-11 0.21115412009519474 1105 12-13 0.1785308004717976 1105 14-15 0.19483473821068742 1105 16-17 0.21420117343523515 1105 18-19 0.2032723629909654 1105 20-21 0.22222153950976065 1105 22-23 0.2603870151700747 1105 24-25 0.32644061352201703 1105 26-27 0.29419577445725764 1105 28-29 0.3263334335827608 1105 30-31 0.26349631066886303 1105 32-33 0.20232765137932773 1105 34-35 0.3699994518718128 1105 36-37 0.35936174066721804 1105 38-39 0.44168234882106105 1105 40-41 0.46719514857817757 1105 42-43 0.354454262325234 1105 44-45 0.3405944319174239 1105 46-47 0.374837982569602 1105 48-49 0.5456247157258858 1105 50-51 0.44967842159645244 1105 52-53 0.5309467868277906 1105 54-55 0.4468524719166709 1105 56-57 0.5913658992973794 1105 58-59 0.4413781072452849 1105 60-61 0.5043392654188992 1105 62-63 0.5798741686874891 1105 64-65 0.5592000112936688 1105 66-67 0.5524601825631237 1105 68-69 0.5908404134394161 1105 70-71 0.6027340463144739 1105 72-73 0.5632954703378417 1105 74-75 0.49682655423433175 1105 76-77 0.718154728099055 1105 78-79 0.6434896021194341 1105 80-81 0.5428827232991047 1105 82-83 0.7243491396642483 1105 84-85 0.761447363785468 1105 86-87 0.7680123741153615 1105 88-89 0.7719669149340582 1105 90-91 0.9102246672797172 1105 92-93 0.8992796281030095 1105 94-95 1.0122895302281485 1105 96-97 1.052750886091939 1105 98-99 1.0238655314691272 1105 100-101 0.9322455899410649 1106 1 0.03537435280744461 1106 2 0.04297588066047808 1106 3 0.12735398605963155 1106 4 0.049699365636101334 1106 5 0.06692524482519957 1106 6 0.08178231810796888 1106 7 0.14190100924083993 1106 8 0.06272408268116436 1106 9 0.13356834259045058 1106 10-11 0.14601648601070139 1106 12-13 0.22776205376029424 1106 14-15 0.15816862678669708 1106 16-17 0.07475065216811316 1106 18-19 0.14892468060035924 1106 20-21 0.12838129604907067 1106 22-23 0.22815517755476833 1106 24-25 0.1861564350299929 1106 26-27 0.12892118051151868 1106 28-29 0.16366583622360054 1106 30-31 0.21433142210077705 1106 32-33 0.12079793135383454 1106 34-35 0.22232499329952304 1106 36-37 0.13011737307541438 1106 38-39 0.16473999094335312 1106 40-41 0.27050624576415316 1106 42-43 0.2595286516243789 1106 44-45 0.28895025257933327 1106 46-47 0.26209701385620576 1106 48-49 0.1975195474741298 1106 50-51 0.23643932087807684 1106 52-53 0.299015309062618 1106 54-55 0.18139993591182701 1106 56-57 0.29779534513874495 1106 58-59 0.36624134568499755 1106 60-61 0.40936606248826735 1106 62-63 0.45034715613106613 1106 64-65 0.49219173817772344 1106 66-67 0.6152779598473614 1106 68-69 0.5320132446218508 1106 70-71 0.3575407293211441 1106 72-73 0.4042477908556421 1106 74-75 0.47085917606382566 1106 76-77 0.43942501759201136 1106 78-79 0.4499777450441833 1106 80-81 0.45239376601842096 1106 82-83 0.501262942204086 1106 84-85 0.5707944777133314 1106 86-87 0.6865405579694333 1106 88-89 0.7616431026906909 1106 90-91 0.8594485173324671 1106 92-93 0.9877601932871762 1106 94-95 1.054642913110282 1106 96-97 0.9884741425638239 1106 98-99 1.0006928747915431 1106 100-101 0.6552532602220964 1107 1 0.06290591337688056 1107 2 0.08014043514977232 1107 3 0.09161789834933387 1107 4 -0.01638858884830796 1107 5 0.028551987676756596 1107 6 -0.004035570691385715 1107 7 0.05256689452397012 1107 8 0.08852194852737938 1107 9 0.13979499265271755 1107 10-11 0.1012151283568059 1107 12-13 0.06777377451334843 1107 14-15 0.07703293465181815 1107 16-17 0.1334948223143826 1107 18-19 0.13717950930643497 1107 20-21 0.15579686999969056 1107 22-23 0.1123761137499173 1107 24-25 0.18519801928511725 1107 26-27 0.16196515775371978 1107 28-29 0.04104966646802666 1107 30-31 0.13523465263333634 1107 32-33 0.12375864241285939 1107 34-35 0.2345951566575195 1107 36-37 0.20991006225569464 1107 38-39 0.027234802068299757 1107 40-41 0.06762123123530728 1107 42-43 0.03214604229172835 1107 44-45 0.032064310157529974 1107 46-47 0.01620538198552879 1107 48-49 -0.03656586751377944 1107 50-51 -0.050157509634949804 1107 52-53 -0.004637658973912551 1107 54-55 0.01914076075982507 1107 56-57 -0.006455869668478442 1107 58-59 0.04504997997308635 1107 60-61 -0.13361613661802352 1107 62-63 -0.15341203123005442 1107 64-65 -0.24710697009761162 1107 66-67 -0.04386178571462551 1107 68-69 0.05723728193291322 1107 70-71 0.06956426850020847 1107 72-73 0.14397992281178773 1107 74-75 -0.012951533774284485 1107 76-77 0.07439070997108033 1107 78-79 0.18430123782859553 1107 80-81 0.11136868849052561 1107 82-83 0.268465589140817 1107 84-85 0.21097346337982614 1107 86-87 0.42085184163607536 1107 88-89 0.5908070223058601 1107 90-91 0.49362061705653915 1107 92-93 0.3286937024318064 1107 94-95 0.4903069670257416 1107 96-97 0.4499848987965791 1107 98-99 0.4000592215793226 1107 100-101 0.2730895603710479 1108 1 -0.025312345442024764 1108 2 -0.018349626681931852 1108 3 0.012430276390254846 1108 4 0.036864237163115376 1108 5 -0.02020480514861589 1108 6 -0.022779702754164077 1108 7 0.0445283227505584 1108 8 0.009393789403546293 1108 9 -0.04153836982276715 1108 10-11 0.0036217500095645505 1108 12-13 0.024856714240208078 1108 14-15 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-0.5464856394139339 2316 80-81 -0.4350027100086358 2316 82-83 -0.28863176588703965 2316 84-85 -0.31662721590844356 2316 86-87 -0.33992831621707964 2316 88-89 -0.6314719530922481 2316 90-91 -0.6571816412882328 2316 92-93 -0.5921818130057268 2316 94-95 -0.3354185826649285 2316 96-97 -0.017197314769763494 2316 98-99 -0.0626611599560043 2316 100-101 0.41881343021437445 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 13.0 4 18.0 5 50.0 6 98.0 7 247.0 8 566.0 9 1094.0 10 1821.0 11 2918.0 12 4159.0 13 5949.0 14 7467.0 15 9981.0 16 12777.0 17 17565.0 18 17341.0 19 12996.0 20 12198.0 21 13152.0 22 14562.0 23 16260.0 24 18879.0 25 22270.0 26 26636.0 27 32901.0 28 39724.0 29 48367.0 30 59537.0 31 72525.0 32 90602.0 33 116694.0 34 159306.0 35 230721.0 36 369042.0 37 589048.0 38 431930.0 39 25394.0 40 4.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.839894245032646 19.56648536282632 15.464040283762372 23.129580108378658 2 15.466624275851522 20.6079728269509 39.85097482106314 24.074428076134442 3 19.253385060859664 24.222366655062853 29.697703853204366 26.826544430873124 4 13.371498481778321 17.3117515790914 36.848819731046376 32.4679302080839 5 14.28818644446367 36.9984485766546 33.93467924171417 14.778685737167555 6 31.957147715222263 36.16888983686914 17.49691626942046 14.377046178488136 7 28.871767113447078 32.289365606695064 20.454520759090716 18.38434652076714 8 25.975132957584368 35.45499363131662 19.708589975511256 18.86128343558776 9 26.102063936349385 17.475586713699006 20.227984779550994 36.19436457040061 10-11 25.01679199457505 26.703738507695746 27.13077231101712 21.14869718671209 12-13 25.82435714530056 23.843344474337126 27.640689548316473 22.691608832045844 14-15 22.73078959320133 25.45471338603687 25.943207435508864 25.87128958525294 16-17 22.384840415149604 28.66373872088236 26.476673490848928 22.474747373119108 18-19 22.275651215744922 27.43792895564819 28.515896964521286 21.7705228640856 20-21 23.1563114356602 26.502616895020353 28.46906107698159 21.87201059233786 22-23 22.763465288160287 26.34638796047191 28.395132031962138 22.49501471940567 24-25 22.62586953153454 26.58119417340929 28.434491098934934 22.35844519612124 26-27 22.49574163040471 26.735779862665694 28.477762406620215 22.29071610030938 28-29 22.325364262530613 26.99265337660953 28.49113515493105 22.19084720592881 30-31 22.544414775345448 26.617192829244836 28.349152753826743 22.489239641582977 32-33 22.4505244857263 26.65212500729431 28.275847497701033 22.621503009278356 34-35 22.877528725443696 26.759390037204582 28.09711706233302 22.2659641750187 36-37 22.839467291737645 26.46766141121123 28.26352633969439 22.429344957356737 38-39 22.555158644237512 26.70484501006543 28.31092725119952 22.429069094497535 40-41 22.7542437598514 26.60700641697822 28.204177787061123 22.434572036109262 42-43 22.651597515545948 26.62773413596967 27.916564294716117 22.804104053768263 44-45 22.717485880049036 26.790197288937218 27.863691865121364 22.62862496589238 46-47 22.78218633844331 26.981337018655736 27.559972343984175 22.67650429891678 48-49 22.866737752099915 26.80723043089725 27.644459612198713 22.681572204804123 50-51 22.758423769344507 27.058041366476527 27.53274490565491 22.650789958524058 52-53 22.956089058595346 27.119540359870065 27.221238133163716 22.703132448370873 54-55 22.72541416564211 26.94983328738759 27.374854868471093 22.949897678499205 56-57 22.787462461676903 27.087014054283777 27.364200010141687 22.761323473897637 58-59 22.91095309473115 26.859075320448 27.44365811877066 22.78631346605019 60-61 22.90170526974532 26.937869182498776 27.63190964253626 22.52851590521964 62-63 22.70449958447109 26.92752089214962 27.539485307581945 22.828494215797342 64-65 22.553959816678283 26.86875304852037 27.525261468473268 23.052025666328078 66-67 22.522961588086293 26.963355631555352 27.377573746318618 23.136109034039734 68-69 22.51339105419828 26.87901859907207 27.482051188146045 23.125539158583607 70-71 22.644024332601084 26.790772510382567 27.388925813174385 23.176277343841964 72-73 22.718250654475284 26.686433396450038 27.413912102108206 23.181403846966475 74-75 22.495482057111303 27.082162471283066 27.102767631145102 23.319587840460525 76-77 22.567022545253824 26.830766648381477 27.255952756830066 23.346258049534637 78-79 22.6349362919481 26.910954814882338 27.258204495592526 23.19590439757704 80-81 22.48290161362406 27.03435533615139 27.214952609062138 23.267790441162415 82-83 22.6758531319233 26.929348865006048 27.182908989200406 23.21188901387025 84-85 22.597175242422473 26.844734919903495 27.111153144197857 23.446936693476175 86-87 22.494568101085495 26.83724689244619 27.21102358207596 23.45716142439236 88-89 22.51194191529961 26.7906510391471 27.135966247337066 23.56144079821622 90-91 22.444099159545168 26.849696810711006 27.213201093701127 23.493002936042696 92-93 22.4916744304908 26.71705941891046 27.202850111577725 23.588416039021013 94-95 22.3733356406815 26.613590951439043 27.252411145296527 23.76066226258293 96-97 22.35401830719792 26.601940184681755 27.252994689745513 23.79104681837481 98-99 22.289569132479812 26.609190834318486 26.960827024171845 24.140413009029857 100-101 22.33838846652106 26.01673131973144 27.663221320339133 23.981658893408365 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 241.0 1 161.0 2 111.5 3 146.5 4 134.0 5 152.0 6 274.0 7 645.5 8 1115.0 9 1349.0 10 1313.5 11 1266.5 12 1417.5 13 1589.5 14 1767.0 15 1943.5 16 2083.0 17 2289.5 18 2486.5 19 2685.5 20 2998.5 21 3484.5 22 4194.5 23 5266.0 24 6463.5 25 8089.5 26 10590.0 27 13658.0 28 16660.5 29 20414.0 30 25071.5 31 29855.5 32 34904.5 33 39727.0 34 44680.5 35 49402.5 36 55014.0 37 61022.0 38 65504.0 39 69214.5 40 73018.5 41 77971.0 42 82716.5 43 86810.0 44 91095.0 45 95232.0 46 97304.0 47 100851.5 48 108424.5 49 107956.0 50 100425.5 51 95454.0 52 92122.5 53 89666.5 54 86940.5 55 80584.5 56 71586.0 57 64336.0 58 57722.0 59 49846.0 60 41720.0 61 33870.0 62 26698.5 63 20599.5 64 15437.5 65 11155.5 66 8311.0 67 6767.0 68 5298.5 69 3962.0 70 2979.0 71 2270.5 72 1820.0 73 1446.5 74 1075.5 75 810.5 76 536.5 77 274.0 78 163.0 79 121.5 80 71.5 81 38.0 82 24.5 83 16.5 84 9.5 85 4.0 86 1.0 87 1.5 88 2.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2974064467576057 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.001428677788889716 16-17 9.65866674178963E-4 18-19 4.024444475745679E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.0061111189364198E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0016299000126770002 36-37 0.007666566726295519 38-39 0.002937844467294346 40-41 0.003159188913460358 42-43 1.4085555665109878E-4 44-45 0.0013280666769960741 46-47 1.2073333427237037E-4 48-49 4.6281111471075314E-4 50-51 4.024444475745679E-5 52-53 6.036666713618519E-5 54-55 0.0 56-57 8.451333399065925E-4 58-59 0.002072588905009025 60-61 7.042777832554939E-4 62-63 0.0012073333427237037 64-65 1.2073333427237037E-4 66-67 5.231777818469383E-4 68-69 0.0016299000126770002 70-71 2.0122222378728396E-5 72-73 0.0 74-75 2.0122222378728396E-5 76-77 0.2611260798087584 78-79 1.4085555665109878E-4 80-81 1.8110000140855555E-4 82-83 0.0 84-85 0.0 86-87 0.10938440085076756 88-89 0.37811668071868526 90-91 0.28130866885462297 92-93 0.0 94-95 0.0 96-97 0.0 98-99 1.0061111189364198E-4 100-101 5.634222266043951E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2484815.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.40302142227915 #Duplication Level Percentage of deduplicated Percentage of total 1 73.72328971138369 40.107697094892984 2 14.591480917934652 15.876412979223526 3 4.995235524028688 8.152677156691876 4 2.270119625990827 4.940054665756612 5 1.2273687138467773 3.3386283216220716 6 0.731140123786966 2.3865739090242086 7 0.48390153855618667 1.8427994037842244 8 0.32558025054272227 1.4170039475957388 9 0.24602794019183727 1.2046196970662164 >10 1.2815563344506455 12.610273791528314 >50 0.08652560686274478 3.2050834389074905 >100 0.035403110239386186 3.406349134682698 >500 0.0016297890021684594 0.5625016499106806 >1k 6.667318645197602E-4 0.7108147595779775 >5k 7.408131827997335E-5 0.23851004973535583 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5918 0.2381666240746293 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4654 0.1872976459012039 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3119 0.12552242319850773 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2721 0.10950513418503995 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 1.2073333427237037E-4 0.0 24-25 0.0 0.0 0.0 3.018333356809259E-4 0.0 26-27 0.0 0.0 0.0 5.634222266043951E-4 0.0 28-29 0.0 0.0 0.0 0.0019518555707366543 0.0 30-31 0.0 0.0 0.0 0.006539722273086728 0.0 32-33 0.0 0.0 0.0 0.01654046679531474 0.0 34-35 0.0 0.0 0.0 0.030002233566684036 0.0 36-37 0.0 0.0 0.0 0.054148900421158115 0.0 38-39 0.0 0.0 0.0 0.0879341117950431 0.0 40-41 2.0122222378728396E-5 0.0 0.0 0.12524071208520554 0.0 42-43 4.024444475745679E-5 0.0 0.0 0.16542479017552614 0.0 44-45 4.024444475745679E-5 0.0 0.0 0.21736024613502414 0.0 46-47 4.024444475745679E-5 0.0 0.0 0.2811476910755931 0.0 48-49 4.024444475745679E-5 0.0 0.0 0.34861750271146946 0.0 50-51 4.024444475745679E-5 0.0 0.0 0.4202928588245 0.0 52-53 4.024444475745679E-5 0.0 0.0 0.49864879276726837 0.0 54-55 4.024444475745679E-5 0.0 0.0 0.5920762712717043 0.0 56-57 4.024444475745679E-5 0.0 0.0 0.7045594943687961 0.0 58-59 4.024444475745679E-5 0.0 0.0 0.8277678619937501 0.0 60-61 4.024444475745679E-5 0.0 0.0 0.9602123296905403 0.0 62-63 4.024444475745679E-5 0.0 0.0 1.1017721641248945 0.0 64-65 4.024444475745679E-5 0.0 0.0 1.2518638208478299 0.0 66-67 4.024444475745679E-5 0.0 0.0 1.4209508554962844 0.0 68-69 4.024444475745679E-5 0.0 0.0 1.6186516903672907 0.0 70-71 4.024444475745679E-5 0.0 0.0 1.8404991920927714 0.0 72-73 4.024444475745679E-5 0.0 0.0 2.0739773383531572 0.0 74-75 4.024444475745679E-5 0.0 0.0 2.3173757402462556 0.0 76-77 4.024444475745679E-5 0.0 0.0 2.589971486810889 0.0 78-79 6.036666713618519E-5 0.0 0.0 2.9092910337389304 0.0 80-81 8.048888951491358E-5 0.0 0.0 3.247726691926763 0.0 82-83 8.048888951491358E-5 0.0 0.0 3.6021192724609277 0.0 84-85 8.048888951491358E-5 0.0 0.0 3.9671565086334395 0.0 86-87 8.048888951491358E-5 0.0 0.0 4.357326400556984 0.0 88-89 8.048888951491358E-5 0.0 0.0 4.786734626119047 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8560 0.0 26.740711 1 GTATCAA 11380 0.0 26.303286 1 ATCAACG 13570 0.0 21.998398 3 TCAACGC 13530 0.0 21.958202 4 TATCAAC 13725 0.0 21.784542 2 CAACGCA 13705 0.0 21.677818 5 GAGTACT 6795 0.0 21.337854 12-13 GTACATG 8805 0.0 21.186457 1 AACGCAG 14050 0.0 21.145514 6 TACATGG 8870 0.0 20.653038 2 GTACTTT 7095 0.0 20.000818 14-15 ACATGGG 9175 0.0 19.242308 3 ACGCAGA 15370 0.0 19.205994 7 CGCAGAG 15500 0.0 19.014292 8 AGTACTT 7070 0.0 18.728966 12-13 GGACCGA 385 1.2732926E-11 18.490591 6 AGAGTAC 12600 0.0 18.475368 10-11 GCAGAGT 16760 0.0 17.329964 9 CCGTAAG 310 1.0945041E-7 16.886227 1 ACTTTTT 8875 0.0 16.123077 16-17 >>END_MODULE