FastQCFastQC Report
Fri 27 May 2016
SRR939274_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939274_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2530692
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55590.21966323835535895No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42910.1695583658540826No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28990.11455364777697168No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25510.10080246825769394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA114100.027.8081511
GGTATCA81900.025.7501831
ATCAACG132400.023.3540173
TCAACGC133250.023.2050444
TATCAAC134250.023.138172
CAACGCA133850.023.0655925
AATACCG1252.2814074E-422.7637215
AACGCAG137700.022.4404856
GTACATG96400.022.1726071
TACATGG95650.021.914892
ACATGGG99650.020.321353
GAGTACT61800.020.05482912-13
ACGCAGA154250.019.8176947
CGCAGAG155100.019.7090878
AGTACTT63000.019.63521812-13
AGAGTAC123550.018.73836910-11
GTACTTT66200.018.4360514-15
GCAGAGT164700.018.2433959
CATGGGG52150.017.369254
GTGTAGC14900.016.8954931
Produced by FastQC (version 0.11.5)