##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939274_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2530692 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.85799931402162 33.0 31.0 34.0 30.0 34.0 2 31.937672778828873 33.0 31.0 34.0 30.0 34.0 3 31.99438730592265 34.0 31.0 34.0 30.0 34.0 4 35.487308214512076 37.0 35.0 37.0 33.0 37.0 5 35.529673701896556 37.0 35.0 37.0 33.0 37.0 6 35.61969611473858 37.0 35.0 37.0 33.0 37.0 7 35.54123338596716 37.0 35.0 37.0 33.0 37.0 8 35.52170434015677 37.0 35.0 37.0 33.0 37.0 9 37.15262228671051 39.0 37.0 39.0 33.0 39.0 10-11 37.06186213099026 39.0 37.0 39.0 33.0 39.0 12-13 36.9638172088899 39.0 37.0 39.0 33.0 39.0 14-15 38.20865241601902 40.0 38.0 41.0 33.0 41.0 16-17 38.144654110417235 40.0 38.0 41.0 33.0 41.0 18-19 38.07706528491022 40.0 38.0 41.0 33.0 41.0 20-21 37.97129954968838 40.0 38.0 41.0 33.0 41.0 22-23 37.89300831551212 40.0 38.0 41.0 32.5 41.0 24-25 37.926321930918505 40.0 38.0 41.0 33.0 41.0 26-27 37.85168977497064 40.0 38.0 41.0 32.5 41.0 28-29 37.72116717482807 40.0 38.0 41.0 32.0 41.0 30-31 37.5662417631225 40.0 37.5 41.0 32.0 41.0 32-33 37.38605270811304 40.0 37.0 41.0 31.5 41.0 34-35 37.10247928234649 40.0 37.0 41.0 30.5 41.0 36-37 36.87408384742197 40.0 36.0 41.0 30.0 41.0 38-39 36.570159071115725 39.5 35.5 41.0 30.0 41.0 40-41 36.455129861713715 39.0 36.0 41.0 30.0 41.0 42-43 36.3023269919848 39.0 35.5 41.0 30.0 41.0 44-45 36.29545436584144 39.5 36.0 41.0 30.0 41.0 46-47 36.15003188851112 39.5 36.0 41.0 29.0 41.0 48-49 35.97984029664613 39.0 35.5 41.0 28.0 41.0 50-51 34.85054957300217 38.0 34.0 40.0 25.5 40.5 52-53 34.97041165025219 38.5 34.5 39.5 26.5 40.5 54-55 35.47678540098914 39.0 35.0 40.5 27.0 41.0 56-57 35.37870017370743 39.0 35.0 41.0 26.5 41.0 58-59 35.177529900912475 39.0 35.0 41.0 26.0 41.0 60-61 34.88455351342637 38.0 34.5 40.0 26.0 41.0 62-63 34.5314886205038 38.0 34.0 40.0 25.5 41.0 64-65 34.1541807537227 37.0 34.0 40.0 25.0 41.0 66-67 33.79679727916317 37.0 34.0 39.5 23.5 41.0 68-69 33.38101041138155 36.0 33.0 39.0 22.5 41.0 70-71 32.969894597999286 36.0 33.0 39.0 22.0 40.5 72-73 32.528109702800656 35.0 32.0 38.0 21.0 40.0 74-75 32.0762008968298 35.0 32.0 37.0 20.0 39.5 76-77 31.624713517093348 35.0 31.5 37.0 18.5 39.0 78-79 31.19811577228679 35.0 31.0 36.0 17.5 39.0 80-81 30.741448584023658 35.0 31.0 36.0 12.0 37.5 82-83 30.305640907704294 34.0 30.5 35.0 7.5 37.0 84-85 29.892886807244818 34.0 30.0 35.0 4.5 36.5 86-87 29.55983936409488 34.0 30.0 35.0 2.0 36.0 88-89 29.1553395276865 34.0 29.5 35.0 2.0 36.0 90-91 28.764854039922675 34.0 29.0 35.0 2.0 35.5 92-93 28.35892396229964 34.0 29.0 35.0 2.0 35.0 94-95 27.89615192208297 34.0 28.5 35.0 2.0 35.0 96-97 27.34037488560441 34.0 27.0 35.0 2.0 35.0 98-99 26.606529557923288 33.5 25.5 35.0 2.0 35.0 100-101 24.07866721829444 31.0 13.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.28843283261553054 1101 2 0.16850684488997913 1101 3 0.3284482114353793 1101 4 0.38840847640332044 1101 5 0.3821151034547796 1101 6 0.38828132934797566 1101 7 0.424810038081894 1101 8 0.37338841416209334 1101 9 0.5473945107684202 1101 10-11 0.5503135375792283 1101 12-13 0.5099603536548329 1101 14-15 0.5281100596189674 1101 16-17 0.5897826020629324 1101 18-19 0.6391377525830322 1101 20-21 0.6760488005891858 1101 22-23 0.6103410563281884 1101 24-25 0.7126453921079658 1101 26-27 0.639425918512508 1101 28-29 0.5900548642218624 1101 30-31 0.6710274352319416 1101 32-33 0.6845895154264738 1101 34-35 0.70138566086883 1101 36-37 0.5905996115995222 1101 38-39 0.8700384393028671 1101 40-41 0.922173863579502 1101 42-43 0.7255013359176985 1101 44-45 0.8412119773396043 1101 46-47 0.829694536752001 1101 48-49 0.8112715560610013 1101 50-51 0.76277838951939 1101 52-53 0.7880337878018437 1101 54-55 0.772402861514351 1101 56-57 0.7440649000123756 1101 58-59 0.7916847353299303 1101 60-61 0.8622398951462102 1101 62-63 0.9006963378814774 1101 64-65 0.8447374842511053 1101 66-67 0.8428914649317463 1101 68-69 0.9179773195486263 1101 70-71 0.7609893470187075 1101 72-73 0.7880785291432844 1101 74-75 0.6990145567322514 1101 76-77 0.5996041590549765 1101 78-79 0.6566936151318963 1101 80-81 0.7097136026370343 1101 82-83 0.571092097605856 1101 84-85 0.5129127113439651 1101 86-87 0.5762996567940135 1101 88-89 0.5046598197508665 1101 90-91 0.6741830493881515 1101 92-93 0.7150128436767567 1101 94-95 0.6940465062026036 1101 96-97 0.5558252232396228 1101 98-99 0.345397027853231 1101 100-101 0.2006613012396805 1102 1 0.3909838627249549 1102 2 0.41885623595127086 1102 3 0.4572225856213237 1102 4 0.29253747063242486 1102 5 0.2356798430039433 1102 6 0.3707880081839079 1102 7 0.3861191374610726 1102 8 0.521064369402005 1102 9 0.49903237049860394 1102 10-11 0.47874422504774117 1102 12-13 0.5306469189743197 1102 14-15 0.5723228839346888 1102 16-17 0.5945391929568942 1102 18-19 0.5765652598798354 1102 20-21 0.5767902464502157 1102 22-23 0.47546600522677807 1102 24-25 0.6086777832738406 1102 26-27 0.5461979253957452 1102 28-29 0.6347068041741366 1102 30-31 0.5928084026886609 1102 32-33 0.5567076333732999 1102 34-35 0.5605151066918239 1102 36-37 0.5576059244067224 1102 38-39 0.6971540378311474 1102 40-41 0.5960167136598358 1102 42-43 0.5232045056314405 1102 44-45 0.6894080974350558 1102 46-47 0.6924575307719465 1102 48-49 0.8454334453396868 1102 50-51 0.7640618668874026 1102 52-53 0.7276461776367071 1102 54-55 0.7700914138469486 1102 56-57 0.8538889685405664 1102 58-59 0.758225787591229 1102 60-61 0.6991319071633555 1102 62-63 0.8038050864435107 1102 64-65 0.7934988097504743 1102 66-67 0.808321358545733 1102 68-69 0.8331151032208908 1102 70-71 0.7617350711376218 1102 72-73 0.866512662986203 1102 74-75 0.805925844678022 1102 76-77 0.6718502682171135 1102 78-79 0.752094012534517 1102 80-81 0.7286377633854393 1102 82-83 0.5892301284663581 1102 84-85 0.5887727600210333 1102 86-87 0.6670718110394702 1102 88-89 0.7034324350974508 1102 90-91 0.5473849926679577 1102 92-93 0.5276293073638563 1102 94-95 0.547731868856264 1102 96-97 0.39456342237826547 1102 98-99 0.4157315876204919 1102 100-101 0.5000484407964514 1103 1 0.2540544902847053 1103 2 0.34307761317465335 1103 3 0.30632695700922596 1103 4 0.409583061593743 1103 5 0.35439594366170013 1103 6 0.2772862363367139 1103 7 0.34801214532954106 1103 8 0.34944073999167813 1103 9 0.44900302159982886 1103 10-11 0.4066638363482724 1103 12-13 0.3924254934207525 1103 14-15 0.5252425536991581 1103 16-17 0.5652020844119576 1103 18-19 0.5503308327251304 1103 20-21 0.5499746877142186 1103 22-23 0.5499672883154147 1103 24-25 0.5146398970805208 1103 26-27 0.6023973638301001 1103 28-29 0.5934688730805178 1103 30-31 0.5503755660649716 1103 32-33 0.4258247966716624 1103 34-35 0.30097466835257336 1103 36-37 0.43207780013265307 1103 38-39 0.6808110275461701 1103 40-41 0.47412923648025895 1103 42-43 0.41512699101934913 1103 44-45 0.5388569188799437 1103 46-47 0.39495596783590514 1103 48-49 0.3411725932628187 1103 50-51 0.4443284156907623 1103 52-53 0.45224359493361277 1103 54-55 0.5857403791910869 1103 56-57 0.5206821122012784 1103 58-59 0.471490067214134 1103 60-61 0.4972349666204039 1103 62-63 0.45863699481569853 1103 64-65 0.46016440457820806 1103 66-67 0.33013864001922144 1103 68-69 0.3885407713857987 1103 70-71 0.3202312655247681 1103 72-73 0.4367242517866998 1103 74-75 0.35691419710177996 1103 76-77 0.3780749046103722 1103 78-79 0.4525462116362 1103 80-81 0.32615795683093296 1103 82-83 0.20418748486722293 1103 84-85 0.1720931338472731 1103 86-87 0.17899173180913763 1103 88-89 0.22754023636993637 1103 90-91 0.22508185635031452 1103 92-93 0.21560559360954912 1103 94-95 0.16758207254463642 1103 96-97 0.05729897359969627 1103 98-99 -0.031498858529538154 1103 100-101 -0.16802925310505756 1104 1 0.2020600820816476 1104 2 0.2667467338021261 1104 3 0.20477146885333752 1104 4 0.23000820097132646 1104 5 0.1973383257574497 1104 6 0.1593833890256704 1104 7 0.2120805733784863 1104 8 0.31497349782684836 1104 9 0.28094093787330365 1104 10-11 0.21413895395851767 1104 12-13 0.2717854566272777 1104 14-15 0.3640101263037039 1104 16-17 0.37636226973729947 1104 18-19 0.20127980823447444 1104 20-21 0.3783712138675668 1104 22-23 0.44377275823025286 1104 24-25 0.3961968520233441 1104 26-27 0.3232384554722074 1104 28-29 0.2576874873570105 1104 30-31 0.3267028441191968 1104 32-33 0.47144655958825155 1104 34-35 0.41048606886962347 1104 36-37 0.3919448589678751 1104 38-39 0.4757815051766414 1104 40-41 0.32634257482543205 1104 42-43 0.33861060624024475 1104 44-45 0.5364555710865702 1104 46-47 0.605996690680513 1104 48-49 0.5146248376250142 1104 50-51 0.536520101109609 1104 52-53 0.749673191505309 1104 54-55 0.7289800013452705 1104 56-57 0.8214077590833924 1104 58-59 0.7062999598104369 1104 60-61 0.7676121915833178 1104 62-63 0.7075296363282817 1104 64-65 0.7203058200951276 1104 66-67 0.8739198854413672 1104 68-69 0.8840979262768087 1104 70-71 0.706262108190117 1104 72-73 0.7065893205184253 1104 74-75 0.5341851518506573 1104 76-77 0.6745798814634583 1104 78-79 0.6514654972772007 1104 80-81 0.6030065819807753 1104 82-83 0.525956366138324 1104 84-85 0.5078427266826004 1104 86-87 0.6189272757141779 1104 88-89 0.5787413604314118 1104 90-91 0.6744095258603338 1104 92-93 0.535131903959531 1104 94-95 0.4273544467193595 1104 96-97 0.4662085975789232 1104 98-99 0.5439848669817309 1104 100-101 0.49628830364055787 1105 1 0.28143513205826665 1105 2 0.23631708923491246 1105 3 0.403320554057899 1105 4 0.331327114324921 1105 5 0.31505949676348166 1105 6 0.175203706778575 1105 7 0.32079828216701856 1105 8 0.36003810055629515 1105 9 0.43673350517951093 1105 10-11 0.45340163032971503 1105 12-13 0.5044225198090899 1105 14-15 0.4761462807459367 1105 16-17 0.508216782596385 1105 18-19 0.4632559201792006 1105 20-21 0.5321772614577611 1105 22-23 0.5257131992807658 1105 24-25 0.5035422006827801 1105 26-27 0.4546733001345089 1105 28-29 0.5797406810680883 1105 30-31 0.6603016063055946 1105 32-33 0.6112939916972167 1105 34-35 0.7374778998702354 1105 36-37 0.8630919033034274 1105 38-39 0.9577739489830677 1105 40-41 0.7856705894735612 1105 42-43 0.7273970900723725 1105 44-45 0.7538802487678495 1105 46-47 0.7794538595248497 1105 48-49 0.7306915953732229 1105 50-51 0.6371146581304217 1105 52-53 0.5542011315698332 1105 54-55 0.5959024038834215 1105 56-57 0.5040318351139135 1105 58-59 0.5245912117050366 1105 60-61 0.5891234266875784 1105 62-63 0.7089798832646537 1105 64-65 0.5803844722327867 1105 66-67 0.587831895189943 1105 68-69 0.5546716587299372 1105 70-71 0.4678557354428108 1105 72-73 0.616266706684101 1105 74-75 0.6593047572867405 1105 76-77 0.5284213616846287 1105 78-79 0.48773759100273395 1105 80-81 0.5079511036501856 1105 82-83 0.41120680697043 1105 84-85 0.4974237642503958 1105 86-87 0.2861275076237284 1105 88-89 0.45900081826342287 1105 90-91 0.3558725198797461 1105 92-93 0.3550642841316254 1105 94-95 0.4461942997109425 1105 96-97 0.482655349085352 1105 98-99 0.439811487880192 1105 100-101 0.18818081948551324 1106 1 0.15574300722793666 1106 2 0.23338010550360266 1106 3 0.09614704404293661 1106 4 0.2945418967355238 1106 5 0.2284273442903455 1106 6 0.229754258042 1106 7 0.2697369164773349 1106 8 0.18599568518720844 1106 9 0.12565566813707818 1106 10-11 0.22082659450202158 1106 12-13 0.29833087427687843 1106 14-15 0.36825909246576316 1106 16-17 0.34082162389302795 1106 18-19 0.4124182246464656 1106 20-21 0.34694720576750626 1106 22-23 0.3609710889054867 1106 24-25 0.3878121793437188 1106 26-27 0.4427351853106174 1106 28-29 0.36563324122012375 1106 30-31 0.33416804715551507 1106 32-33 0.34436010318895427 1106 34-35 0.40558832095384645 1106 36-37 0.5202510960177378 1106 38-39 0.6241023267338974 1106 40-41 0.7459786929624244 1106 42-43 0.6280377785149938 1106 44-45 0.5918171868697684 1106 46-47 0.5130812987391664 1106 48-49 0.5878842266899014 1106 50-51 0.5177264502483823 1106 52-53 0.40867261483012385 1106 54-55 0.5289076819136866 1106 56-57 0.5844527069316214 1106 58-59 0.6475325724150878 1106 60-61 0.6451706222959999 1106 62-63 0.5342443314700347 1106 64-65 0.5298346723908907 1106 66-67 0.4152021832870929 1106 68-69 0.36040439889254117 1106 70-71 0.3569883446404205 1106 72-73 0.3947632575318707 1106 74-75 0.3894534282056057 1106 76-77 0.32654354826617293 1106 78-79 0.3076425647623857 1106 80-81 0.2949275773033406 1106 82-83 0.38201612962522447 1106 84-85 0.417128846797425 1106 86-87 0.4843349953567362 1106 88-89 0.44328424468360694 1106 90-91 0.41567820061961314 1106 92-93 0.4210264041527907 1106 94-95 0.40790296752356525 1106 96-97 0.46693507349754526 1106 98-99 0.45196827945490625 1106 100-101 0.10516940300944455 1107 1 0.15916017473927369 1107 2 0.19164556387575615 1107 3 0.21796931452442436 1107 4 0.13600372816819117 1107 5 0.28556434153688315 1107 6 0.21658813213669958 1107 7 0.3025263050530498 1107 8 0.07408452690006584 1107 9 0.3011201732836426 1107 10-11 0.2637040053999229 1107 12-13 0.34681701601289205 1107 14-15 0.392384112714268 1107 16-17 0.4496165442677196 1107 18-19 0.43570412384814716 1107 20-21 0.46465387020580806 1107 22-23 0.3230980626264568 1107 24-25 0.2433816401126947 1107 26-27 0.24959102477902206 1107 28-29 0.3194620190762123 1107 30-31 0.3826637224738647 1107 32-33 0.3808636925412543 1107 34-35 0.4229852019967879 1107 36-37 0.3610091003720157 1107 38-39 0.42596721149941885 1107 40-41 0.37742341534534063 1107 42-43 0.3474052593164316 1107 44-45 0.44033801426532904 1107 46-47 0.44470254990662283 1107 48-49 0.41265399896661137 1107 50-51 0.2901212082039919 1107 52-53 0.4924197470871121 1107 54-55 0.5519924639784861 1107 56-57 0.4451009971623918 1107 58-59 0.48348913310314146 1107 60-61 0.4741166839399753 1107 62-63 0.6922147959345608 1107 64-65 0.7187449275576441 1107 66-67 0.47175945582700507 1107 68-69 0.5679414501240174 1107 70-71 0.522415977206542 1107 72-73 0.43583169190707594 1107 74-75 0.6127913558966185 1107 76-77 0.44184551163699126 1107 78-79 0.4891136412828949 1107 80-81 0.46515789704642785 1107 82-83 0.5471969667599659 1107 84-85 0.3610048962997645 1107 86-87 0.33893360584125176 1107 88-89 0.30293814082658344 1107 90-91 0.43097338271186203 1107 92-93 0.35812142379279877 1107 94-95 0.37878740707576597 1107 96-97 0.47264718152845475 1107 98-99 0.5973506018057186 1107 100-101 0.39506102027143086 1108 1 0.025540388831938543 1108 2 0.11020811192501512 1108 3 0.07400056772571162 1108 4 0.22422001517988832 1108 5 0.2361225506721496 1108 6 0.04291189822213681 1108 7 0.09866245044688071 1108 8 0.17861721762989902 1108 9 0.17008248160119877 1108 10-11 0.22629762666021946 1108 12-13 0.15761781945874986 1108 14-15 0.1615183691678297 1108 16-17 0.13157711682820405 1108 18-19 0.15300310202049872 1108 20-21 0.25090898149818486 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2316 72-73 -1.2595432363916146 2316 74-75 -1.2354305577164872 2316 76-77 -0.9443083774999508 2316 78-79 -0.9926509231518814 2316 80-81 -0.8105632737332336 2316 82-83 -0.602676566177081 2316 84-85 -0.4769794218373953 2316 86-87 -0.4445818209953458 2316 88-89 -0.6197104536689793 2316 90-91 -0.6773788061442048 2316 92-93 -0.6734593257777881 2316 94-95 -0.8109898541171852 2316 96-97 -0.6877773007788726 2316 98-99 -0.6799493217658572 2316 100-101 -0.15469895580959658 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7702.0 3 2295.0 4 2281.0 5 2469.0 6 2802.0 7 3404.0 8 4158.0 9 5200.0 10 6317.0 11 7860.0 12 9669.0 13 13489.0 14 12382.0 15 12644.0 16 15434.0 17 19242.0 18 16826.0 19 13348.0 20 14019.0 21 14839.0 22 16138.0 23 18182.0 24 20716.0 25 23879.0 26 27603.0 27 32366.0 28 37725.0 29 45364.0 30 54326.0 31 66540.0 32 83186.0 33 107316.0 34 144904.0 35 208580.0 36 329238.0 37 531370.0 38 515042.0 39 81811.0 40 26.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.69331584547016 18.94811295521773 15.042151165670425 23.316420033641684 2 14.935796510937932 20.050990941097353 40.91668974247399 24.096522805490725 3 19.26841137024509 24.097165351215555 29.450952465729035 27.183470812810317 4 12.9547177335762 16.748061015693864 37.316922059240106 32.980299191489834 5 14.334826676331257 36.90451085070698 33.85058947021076 14.910073002751007 6 31.815362109695467 35.770210831167034 17.926480908560755 14.487946150576747 7 28.822872805448057 32.265552992886256 20.624488196542018 18.28708600512367 8 25.90558491813495 35.12838296852646 20.036829980252424 18.929202133086164 9 26.251342931130818 17.580624306292293 20.433668395556634 35.73436436702026 10-11 24.958443945191874 26.57406902179973 27.30586679762162 21.16162023538678 12-13 25.84449220147845 23.81041160456385 27.703583029689543 22.64151316426816 14-15 22.82919467213682 25.33848743376646 26.046836862955047 25.78548103114167 16-17 22.4492955076143 28.501629340666142 26.555079780656328 22.49399537106323 18-19 22.35415557437305 27.3724228890768 28.557984365574846 21.715437170975306 20-21 23.25033305428874 26.43921171022438 28.432594517623897 21.87786071786298 22-23 22.82378013779254 26.285143909423837 28.337518362023467 22.553557590760153 24-25 22.64458426720993 26.504238385818287 28.432621866693243 22.418555480278542 26-27 22.484759292935603 26.665977173150413 28.442240350320713 22.40702318359327 28-29 22.357046685780034 26.938540807123633 28.487997195282045 22.21641531181428 30-31 22.57077459260639 26.551698364810015 28.347702393762482 22.529824648821112 32-33 22.493983971889413 26.56509163481943 28.291376838543865 22.64954755474729 34-35 22.914680825233344 26.735339068664548 27.996408205752616 22.353571900349493 36-37 22.889821015441562 26.372227837322963 28.246190869667725 22.49176027756775 38-39 22.5901844606457 26.73271841164968 28.206829532100215 22.47026759560441 40-41 22.803040513755647 26.554091017204474 28.178704511218584 22.464163957821295 42-43 22.69229686451105 26.518686169185635 27.88726323840731 22.901753727896008 44-45 22.735876329624286 26.7454993137487 27.864757139214436 22.653867217412582 46-47 22.80528232118191 26.914988163993236 27.537880575504758 22.741848939320093 48-49 22.88943142604267 26.734380909073423 27.621766946689608 22.7544207181943 50-51 22.77927658616727 26.952633358418133 27.559685458451945 22.70840459696266 52-53 22.932438177791596 27.027108491074888 27.295448920927225 22.745004410206295 54-55 22.755164558016144 26.83397078621833 27.468687481535675 22.94217717422985 56-57 22.749448084893732 27.051580993702906 27.418202880761953 22.78076804064141 58-59 22.936501691714533 26.723183336824913 27.543165923194913 22.797149048265645 60-61 22.945442464385383 26.800868590691596 27.71013113453072 22.543557810392304 62-63 22.71966887201899 26.781172904855822 27.66725904285647 22.83189918026872 64-65 22.549969162794664 26.755925998639597 27.582779552652298 23.111325285913445 66-67 22.558746808785333 26.901238091139483 27.38747875746253 23.152536342612652 68-69 22.56519996294944 26.739774749249218 27.613041400828557 23.081983886972782 70-71 22.70277614418138 26.7007093830361 27.521879998928934 23.074634473853585 72-73 22.745928460203977 26.61832353540778 27.536903844948718 23.098844159439523 74-75 22.575189483408078 26.971672582507434 27.284103542880622 23.169034391203862 76-77 22.70217686837426 26.724319123859257 27.35590124381148 23.217602763955007 78-79 22.748991236434673 26.80424654074485 27.35552289378851 23.09123932903197 80-81 22.61647897129934 26.98697789048487 27.27480520757506 23.121737930640734 82-83 22.81264839218462 26.833867173443014 27.317961513944777 23.03552292042759 84-85 22.75574874403146 26.735707180132767 27.232126304126307 23.27641777170946 86-87 22.684935787307115 26.73894134560651 27.351218735557275 23.224904131529104 88-89 22.718206832767653 26.692109819872627 27.26765440990419 23.32202893745553 90-91 22.65647670336135 26.81174756178133 27.350951357014402 23.180824377842917 92-93 22.788305633871257 26.629822200493265 27.356153366330055 23.225718799305422 94-95 22.677299316891826 26.53005164267663 27.37478366522186 23.41786537520969 96-97 22.694582641850438 26.470371805071345 27.463171655756657 23.37187389732156 98-99 22.663550058079426 26.546770052078116 27.131411816946976 23.65826807289548 100-101 22.821282713798023 26.063292000135625 27.598549607074542 23.51687567899181 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1672.0 1 930.0 2 215.0 3 226.0 4 217.5 5 252.0 6 391.5 7 807.5 8 1319.0 9 1629.0 10 1612.5 11 1581.0 12 1759.5 13 2007.5 14 2273.0 15 2491.0 16 2646.0 17 2785.0 18 2953.5 19 3158.0 20 3394.5 21 3787.5 22 4409.0 23 5291.0 24 6519.0 25 8140.0 26 10550.0 27 13832.5 28 16854.5 29 20554.5 30 25408.5 31 30565.0 32 35838.5 33 40531.5 34 45337.0 35 50272.5 36 55972.5 37 61925.0 38 66453.5 39 70190.0 40 74267.0 41 79368.5 42 84214.5 43 88394.0 44 92400.5 45 96524.0 46 98925.5 47 102413.0 48 110109.5 49 109717.0 50 101785.0 51 96341.5 52 92560.0 53 89918.5 54 87409.0 55 81477.5 56 72584.5 57 64828.0 58 58305.0 59 50899.5 60 42736.5 61 34684.5 62 27348.0 63 21213.0 64 15905.0 65 11741.5 66 8874.5 67 7140.5 68 5670.5 69 4297.0 70 3253.5 71 2444.5 72 1897.5 73 1522.0 74 1118.0 75 801.0 76 578.0 77 351.0 78 227.0 79 152.0 80 94.0 81 66.0 82 49.5 83 33.0 84 21.0 85 20.0 86 16.5 87 14.5 88 12.0 89 9.0 90 9.0 91 6.5 92 3.0 93 3.5 94 5.0 95 4.5 96 3.5 97 2.0 98 2.5 99 2.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2780662364286132 2 0.11032555522363054 3 0.1202042761426519 4 0.12170584172234314 5 0.1285419165983059 6 0.05599259016901306 7 0.06500198364716053 8 0.07009940364137557 9 0.06753093620243 10-11 0.06235448644086282 12-13 0.06666160876155612 14-15 0.06215691202248239 16-17 0.06328308620725083 18-19 0.06802487224838108 20-21 0.07211466270885591 22-23 0.0804325457226719 24-25 0.08902703292222049 26-27 0.0895407264100096 28-29 0.08280343874323703 30-31 0.07883219293379044 32-33 0.0720158754996657 34-35 0.23900577391480277 36-37 0.3642284402843175 38-39 0.3369829279896566 40-41 0.3197544387068833 42-43 0.3435028837962107 44-45 0.3564637656419667 46-47 0.36152167075250563 48-49 0.371854812833802 50-51 0.3652953421435718 52-53 0.3662239419099598 54-55 0.35314451541317554 56-57 0.32949485753303837 58-59 0.3143606570850977 60-61 0.2700249576005298 62-63 0.34164568426343467 64-65 0.37092621306741397 66-67 0.3758853309687627 68-69 0.38773979607158837 70-71 0.38880669793084266 72-73 0.37183505539196393 74-75 0.37519382050443123 76-77 0.35911126284826445 78-79 0.33765468101215007 80-81 0.3366668089202479 82-83 0.35047726076503977 84-85 0.3569181868042417 86-87 0.3507933798344484 88-89 0.3656707335384946 90-91 0.3890437872328991 92-93 0.3846576351448536 94-95 0.38388709491316997 96-97 0.3888857276981948 98-99 0.3783747686403561 100-101 0.3568391570368895 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2530692.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.48673200317589 #Duplication Level Percentage of deduplicated Percentage of total 1 74.67646178626103 41.435528220796705 2 13.976082715195012 15.509743121444888 3 4.86644039672948 8.100686223082729 4 2.263958375077209 5.024786064970186 5 1.1508623168955956 3.1928794475069995 6 0.7269704595664497 2.4202329038513524 7 0.4599736611324811 1.7865704684644337 8 0.307275611728195 1.3639775615259428 9 0.2338262269986082 1.167682787350699 >10 1.2217092219761179 12.288383714549177 >50 0.07974160580057559 2.9894432959492434 >100 0.03455636647447869 3.324330560154082 >500 0.0014275041097524704 0.5080371485470944 >1k 6.423768493838851E-4 0.6675611300169306 >5k 7.137520548709834E-5 0.22015735178951812 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5559 0.21966323835535895 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4291 0.1695583658540826 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2899 0.11455364777697168 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2551 0.10080246825769394 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 3.951488367608543E-5 0.0 0.0 3.951488367608543E-5 0.0 8 3.951488367608543E-5 0.0 0.0 3.951488367608543E-5 0.0 9 3.951488367608543E-5 0.0 0.0 3.951488367608543E-5 0.0 10-11 3.951488367608543E-5 0.0 0.0 3.951488367608543E-5 0.0 12-13 3.951488367608543E-5 0.0 0.0 5.927232551412815E-5 0.0 14-15 3.951488367608543E-5 0.0 0.0 7.902976735217086E-5 0.0 16-17 3.951488367608543E-5 0.0 0.0 7.902976735217086E-5 0.0 18-19 3.951488367608543E-5 0.0 0.0 1.3830209286629902E-4 0.0 20-21 3.951488367608543E-5 0.0 0.0 1.7781697654238447E-4 0.0 22-23 3.951488367608543E-5 0.0 0.0 2.370893020565126E-4 0.0 24-25 3.951488367608543E-5 0.0 0.0 3.1611906940868346E-4 0.0 26-27 3.951488367608543E-5 0.0 0.0 6.124806969793242E-4 0.0 28-29 3.951488367608543E-5 0.0 0.0 0.002311620695050998 0.0 30-31 3.951488367608543E-5 0.0 0.0 0.006816317434124737 0.0 32-33 3.951488367608543E-5 0.0 0.0 0.016260374632709157 0.0 34-35 3.951488367608543E-5 0.0 0.0 0.030426460430585785 0.0 36-37 3.951488367608543E-5 0.0 0.0 0.05326606319536316 0.0 38-39 3.951488367608543E-5 0.0 0.0 0.08497675734542173 0.0 40-41 3.951488367608543E-5 0.0 0.0 0.12202196079175182 0.0 42-43 3.951488367608543E-5 0.0 0.0 0.16372992051186 0.0 44-45 3.951488367608543E-5 0.0 0.0 0.2135977037110798 0.0 46-47 3.951488367608543E-5 0.0 0.0 0.2770981217785491 0.0 48-49 3.951488367608543E-5 0.0 0.0 0.34617013844434646 0.0 50-51 3.951488367608543E-5 0.0 0.0 0.42253265114838157 0.0 52-53 3.951488367608543E-5 0.0 0.0 0.4974528705982395 0.0 54-55 3.951488367608543E-5 0.0 0.0 0.5884754051461023 0.0 56-57 3.951488367608543E-5 0.0 0.0 0.6950075315368287 0.0 58-59 3.951488367608543E-5 0.0 0.0 0.8158045309346218 0.0 60-61 3.951488367608543E-5 0.0 0.0 0.9472903063667961 0.0 62-63 3.951488367608543E-5 0.0 0.0 1.0839130166768616 0.0 64-65 3.951488367608543E-5 0.0 0.0 1.2300192990691874 0.0 66-67 3.951488367608543E-5 0.0 0.0 1.4022054046877297 0.0 68-69 3.951488367608543E-5 0.0 0.0 1.598950010510959 0.0 70-71 3.951488367608543E-5 0.0 0.0 1.8161435686365626 0.0 72-73 5.927232551412815E-5 0.0 0.0 2.0413388907065735 0.0 74-75 7.902976735217086E-5 0.0 0.0 2.288129096705565 0.0 76-77 7.902976735217086E-5 0.0 0.0 2.5582528415152854 0.0 78-79 7.902976735217086E-5 0.0 0.0 2.862635990472171 0.0 80-81 7.902976735217086E-5 0.0 0.0 3.1971887531157486 0.0 82-83 7.902976735217086E-5 0.0 0.0 3.54653983969602 0.0 84-85 7.902976735217086E-5 0.0 0.0 3.904702745336058 0.0 86-87 7.902976735217086E-5 0.0 0.0 4.293489685825063 0.0 88-89 7.902976735217086E-5 0.0 0.0 4.7188278937144466 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 11410 0.0 27.808151 1 GGTATCA 8190 0.0 25.750183 1 ATCAACG 13240 0.0 23.354017 3 TCAACGC 13325 0.0 23.205044 4 TATCAAC 13425 0.0 23.13817 2 CAACGCA 13385 0.0 23.065592 5 AATACCG 125 2.2814074E-4 22.763721 5 AACGCAG 13770 0.0 22.440485 6 GTACATG 9640 0.0 22.172607 1 TACATGG 9565 0.0 21.91489 2 ACATGGG 9965 0.0 20.32135 3 GAGTACT 6180 0.0 20.054829 12-13 ACGCAGA 15425 0.0 19.817694 7 CGCAGAG 15510 0.0 19.709087 8 AGTACTT 6300 0.0 19.635218 12-13 AGAGTAC 12355 0.0 18.738369 10-11 GTACTTT 6620 0.0 18.43605 14-15 GCAGAGT 16470 0.0 18.243395 9 CATGGGG 5215 0.0 17.36925 4 GTGTAGC 1490 0.0 16.895493 1 >>END_MODULE