FastQCFastQC Report
Fri 27 May 2016
SRR939274_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939274_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2530692
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58950.23294023927052362No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47490.18765618257772973No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32520.12850240171462984No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29040.1147512221953521No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83800.026.2889141
GTATCAA114600.026.281781
GTACATG89200.022.1370091
ATCAACG133800.022.1311633
TCAACGC134950.021.9777324
CAACGCA136100.021.7920275
TATCAAC138650.021.528142
TACATGG90400.021.312452
AACGCAG140550.021.1358266
AGTACTT70050.020.12021412-13
GAGTACT67950.020.0087312-13
ACATGGG93300.019.8362123
GTACTTT70900.018.9754114-15
ACGCAGA155800.018.9451777
CGCAGAG156750.018.7395388
AGAGTAC126250.018.66260310-11
CATGGGG46650.017.7000054
GCAGAGT169400.017.0043339
TGCACCG4507.2759576E-1216.8726465
TACTTTT86550.016.2570614-15
Produced by FastQC (version 0.11.5)