##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939274_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2530692 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.290068882345224 33.0 31.0 34.0 31.0 34.0 2 32.49390601463947 34.0 31.0 34.0 31.0 34.0 3 32.585968976074525 34.0 31.0 34.0 31.0 34.0 4 36.11192748860786 37.0 35.0 37.0 35.0 37.0 5 36.04806827539661 37.0 35.0 37.0 35.0 37.0 6 36.081691489916594 37.0 35.0 37.0 35.0 37.0 7 36.02978197267783 37.0 35.0 37.0 35.0 37.0 8 36.006434998806654 37.0 35.0 37.0 35.0 37.0 9 37.72272643213793 39.0 37.0 39.0 35.0 39.0 10-11 37.638303278312804 39.0 37.0 39.0 35.0 39.0 12-13 37.564137793141164 39.0 37.0 39.0 35.0 39.0 14-15 38.87363179715271 40.0 38.0 41.0 35.0 41.0 16-17 38.817176685270276 40.0 38.0 41.0 35.0 41.0 18-19 38.9237819141958 40.0 38.0 41.0 35.0 41.0 20-21 38.927673142365805 40.0 38.0 41.0 35.0 41.0 22-23 38.86936517758779 40.0 38.0 41.0 35.0 41.0 24-25 38.808191593445585 40.0 38.0 41.0 34.0 41.0 26-27 38.64777815712066 40.0 38.0 41.0 34.0 41.0 28-29 38.50337674438454 40.0 38.0 41.0 34.0 41.0 30-31 38.334641868706264 40.0 38.0 41.0 34.0 41.0 32-33 38.15508386638912 40.0 38.0 41.0 33.5 41.0 34-35 38.12827202994279 40.0 38.0 41.0 33.0 41.0 36-37 38.06005037357371 40.0 38.0 41.0 33.0 41.0 38-39 37.96596800400839 40.0 38.0 41.0 33.0 41.0 40-41 37.813908804390266 40.0 37.0 41.0 33.0 41.0 42-43 37.657852871862715 40.0 37.0 41.0 33.0 41.0 44-45 37.45581307405247 40.0 37.0 41.0 32.0 41.0 46-47 37.20529029214144 40.0 37.0 41.0 31.5 41.0 48-49 36.92741234413354 40.0 36.0 41.0 31.0 41.0 50-51 36.703402863722644 39.5 36.0 41.0 31.0 41.0 52-53 36.480491897077954 39.0 35.5 41.0 30.0 41.0 54-55 36.21031460960086 39.0 35.0 40.5 30.0 41.0 56-57 35.966191263101166 39.0 35.0 40.0 29.0 41.0 58-59 35.67714186475477 38.0 35.0 40.0 28.5 41.0 60-61 35.340529783948426 38.0 34.5 40.0 28.0 41.0 62-63 34.95545842797148 37.5 34.0 40.0 28.0 41.0 64-65 34.52915151270878 37.0 34.0 39.5 26.5 41.0 66-67 34.153865424950965 36.5 33.5 39.0 26.0 40.5 68-69 33.76088279411323 36.0 33.0 39.0 26.0 40.0 70-71 33.36339586168526 35.5 33.0 38.5 26.0 40.0 72-73 32.858977109818184 35.0 32.0 37.5 25.0 40.0 74-75 32.35257727925801 35.0 32.0 37.0 23.5 39.0 76-77 30.33730201067534 33.5 29.5 35.0 20.0 37.5 78-79 31.331126427080022 34.0 31.0 36.0 21.5 38.0 80-81 31.37532421962056 35.0 31.0 36.0 22.0 37.0 82-83 31.071633766574518 34.0 31.0 35.0 20.5 37.0 84-85 30.6947815064022 34.0 31.0 35.0 19.5 36.5 86-87 30.162348677752963 34.0 30.5 35.0 17.0 36.0 88-89 29.66563532820272 34.0 30.0 35.0 9.5 36.0 90-91 29.248198911602046 34.0 29.5 35.0 4.5 35.0 92-93 28.89906891079594 34.0 29.0 35.0 2.0 35.0 94-95 28.502431943515845 34.0 29.0 35.0 2.0 35.0 96-97 27.9410364833018 33.5 29.0 35.0 2.0 35.0 98-99 27.175117517264056 33.0 27.0 35.0 2.0 35.0 100-101 23.75083297374789 30.0 13.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.20897986989247386 1101 2 0.2384780614649955 1101 3 0.18743940994367847 1101 4 0.1337766031974894 1101 5 0.24158920622147662 1101 6 0.18445209982441213 1101 7 0.24398311951294005 1101 8 0.20748566081706343 1101 9 0.2433585966117704 1101 10-11 0.2288500645575624 1101 12-13 0.24670033747147357 1101 14-15 0.3539593644568768 1101 16-17 0.28592133852112056 1101 18-19 0.2349576789760519 1101 20-21 0.26266622452223487 1101 22-23 0.2198637072262173 1101 24-25 0.29427082083840617 1101 26-27 0.37896082639727524 1101 28-29 0.33452930221310595 1101 30-31 0.32577352629049017 1101 32-33 0.3423867542963137 1101 34-35 0.4293013946801807 1101 36-37 0.520192469370997 1101 38-39 0.40947714901002996 1101 40-41 0.6100261599156411 1101 42-43 0.6593433263815598 1101 44-45 0.7030948813016806 1101 46-47 0.5842181364132131 1101 48-49 0.624934220802821 1101 50-51 0.5980089579645167 1101 52-53 0.594295818214448 1101 54-55 0.46073472561673867 1101 56-57 0.581245231139782 1101 58-59 0.39705044701689474 1101 60-61 0.3700552853196797 1101 62-63 0.5229280158656664 1101 64-65 0.514993747590978 1101 66-67 0.47855722724862204 1101 68-69 0.6249920550282653 1101 70-71 0.648257217678136 1101 72-73 0.5323126952702211 1101 74-75 0.36907616812896293 1101 76-77 0.4264057340315084 1101 78-79 0.5925462533918839 1101 80-81 0.6563488471990127 1101 82-83 0.6017870958287617 1101 84-85 0.5749392471957826 1101 86-87 0.6304598979875671 1101 88-89 0.8556154912707719 1101 90-91 0.8225654558152335 1101 92-93 0.726349262057596 1101 94-95 0.9043603061007524 1101 96-97 0.7890032004495318 1101 98-99 0.780160235884626 1101 100-101 0.4960935246599121 1102 1 0.20068123798427706 1102 2 0.19337690443391153 1102 3 0.21063232134571308 1102 4 0.1605204686510362 1102 5 0.13986530597144053 1102 6 0.1545637148192327 1102 7 0.1541361385369271 1102 8 0.21581519125449233 1102 9 0.20824598335677535 1102 10-11 0.2465971101828046 1102 12-13 0.22061663097898787 1102 14-15 0.292579436141466 1102 16-17 0.3460944415891163 1102 18-19 0.3103542497146705 1102 20-21 0.32373538493789766 1102 22-23 0.2534723939911814 1102 24-25 0.2371685562139021 1102 26-27 0.3544802782098273 1102 28-29 0.3485180171195097 1102 30-31 0.2516830126143219 1102 32-33 0.24419776463068388 1102 34-35 0.4274219322195165 1102 36-37 0.29532426348221463 1102 38-39 0.3353462295648697 1102 40-41 0.4220834790641703 1102 42-43 0.2923103933030262 1102 44-45 0.43008439006259636 1102 46-47 0.3804661042407176 1102 48-49 0.33115694690874875 1102 50-51 0.12153818797317228 1102 52-53 0.2574976901899575 1102 54-55 0.21721866313515648 1102 56-57 0.1937865876327507 1102 58-59 0.12989403866193072 1102 60-61 0.08637767106642258 1102 62-63 0.12147412122489243 1102 64-65 0.17780344592625852 1102 66-67 0.28375863812300395 1102 68-69 0.3132847211435106 1102 70-71 0.37380697381744454 1102 72-73 0.3912317934134535 1102 74-75 0.39416933907864404 1102 76-77 0.33646784007526165 1102 78-79 0.5284276458222159 1102 80-81 0.5128673673020643 1102 82-83 0.5597407337740563 1102 84-85 0.4117712447619333 1102 86-87 0.40020049295063487 1102 88-89 0.4219908798791998 1102 90-91 0.5961921350314547 1102 92-93 0.5656828520864039 1102 94-95 0.553530086388438 1102 96-97 0.43748132904445924 1102 98-99 0.42388177358558465 1102 100-101 0.3916642204947891 1103 1 0.12542664700580985 1103 2 0.14863659346810465 1103 3 0.19165336680570988 1103 4 0.10790136201124767 1103 5 0.05714331058931066 1103 6 0.04211923607594059 1103 7 0.09851295968871199 1103 8 0.14783819174870416 1103 9 0.19353606584549965 1103 10-11 0.22414335126315876 1103 12-13 0.20382802801774602 1103 14-15 0.26033170025505115 1103 16-17 0.1925352932294544 1103 18-19 0.1962372150369731 1103 20-21 0.30473762449662445 1103 22-23 0.24298912715084242 1103 24-25 0.23495131122191992 1103 26-27 0.21772697967423937 1103 28-29 0.2739704500769591 1103 30-31 0.27164406393630713 1103 32-33 0.2101385235082347 1103 34-35 0.35950602753289473 1103 36-37 0.4512786795365642 1103 38-39 0.40992702361432976 1103 40-41 0.41220911417745754 1103 42-43 0.5163253148599978 1103 44-45 0.5582173752626503 1103 46-47 0.6609421878114148 1103 48-49 0.4180805509067511 1103 50-51 0.46339770769578337 1103 52-53 0.448517024605259 1103 54-55 0.512286567054737 1103 56-57 0.5156425722356843 1103 58-59 0.5005424700353913 1103 60-61 0.39956423262271556 1103 62-63 0.26926219645162064 1103 64-65 0.4079620508051107 1103 66-67 0.2791045185977552 1103 68-69 0.2879475206153117 1103 70-71 0.26265766626436715 1103 72-73 0.22596972294803663 1103 74-75 0.33602129746478937 1103 76-77 0.32129381630616294 1103 78-79 0.4605928111348625 1103 80-81 0.485184203670066 1103 82-83 0.5480950088913374 1103 84-85 0.46083262566464356 1103 86-87 0.32765784935149966 1103 88-89 0.5759787638119036 1103 90-91 0.6960584864605472 1103 92-93 0.619179112732688 1103 94-95 0.6992995965853304 1103 96-97 0.5597307954635475 1103 98-99 0.4665079309767499 1103 100-101 0.5566733369721106 1104 1 0.1216977820081695 1104 2 0.1218609794198855 1104 3 0.11492690512533699 1104 4 0.028724690901078986 1104 5 0.0777214684812364 1104 6 0.08322340777179704 1104 7 0.11242774086829854 1104 8 0.09039904042937508 1104 9 0.1647495281735516 1104 10-11 0.1427950101860489 1104 12-13 0.18758683272519505 1104 14-15 0.2294444873546766 1104 16-17 0.17176347069416664 1104 18-19 0.06568567501386013 1104 20-21 0.12080244897643411 1104 22-23 0.1561820690921536 1104 24-25 0.10399884318409391 1104 26-27 0.12148653090495998 1104 28-29 0.2474774082178257 1104 30-31 0.2105527632020383 1104 32-33 0.2341072663161512 1104 34-35 0.19694711456081393 1104 36-37 0.14187038551293085 1104 38-39 0.31899608095613274 1104 40-41 0.3745269451484532 1104 42-43 0.42239346118081755 1104 44-45 0.46692967700960963 1104 46-47 0.409820386537028 1104 48-49 0.45341661111329046 1104 50-51 0.42725313277232857 1104 52-53 0.48297348157203857 1104 54-55 0.46233149912767857 1104 56-57 0.40935416403525693 1104 58-59 0.398109910816693 1104 60-61 0.4876145895565074 1104 62-63 0.42900204622037563 1104 64-65 0.3703221785093618 1104 66-67 0.46029584594803197 1104 68-69 0.4366731509913748 1104 70-71 0.2842591987753593 1104 72-73 0.2790336476609738 1104 74-75 0.3317044047122337 1104 76-77 0.40937624748112356 1104 78-79 0.4962608678728806 1104 80-81 0.4249242072451267 1104 82-83 0.47111017137925515 1104 84-85 0.4578469077052638 1104 86-87 0.5836656266984939 1104 88-89 0.8258678638405819 1104 90-91 0.9249820055262461 1104 92-93 0.9123579345051311 1104 94-95 0.9412731115151409 1104 96-97 0.6894490617063873 1104 98-99 0.5872131478802167 1104 100-101 0.2613646730443264 1105 1 0.03839752574884159 1105 2 0.042350733108051486 1105 3 0.09342021868281591 1105 4 0.06656684908945465 1105 5 0.08717402079490455 1105 6 0.07223333558705747 1105 7 0.12392430292837986 1105 8 0.08236884742351691 1105 9 0.10672069796120098 1105 10-11 0.10634903845021171 1105 12-13 0.11509425092464909 1105 14-15 0.24690620413874598 1105 16-17 0.09458035222192507 1105 18-19 -0.02388442410545366 1105 20-21 0.003688849217873269 1105 22-23 -0.013237350191751318 1105 24-25 0.039553872875842444 1105 26-27 0.09651843922559777 1105 28-29 0.08049278648507396 1105 30-31 0.15631619921779816 1105 32-33 0.2624096895073933 1105 34-35 0.20938734343069854 1105 36-37 0.1440035634697523 1105 38-39 0.15536032118251342 1105 40-41 0.262049099567939 1105 42-43 0.21202399252046433 1105 44-45 0.2407695832494099 1105 46-47 0.33231868124013175 1105 48-49 0.3304913739646622 1105 50-51 0.4060780293308852 1105 52-53 0.34086543677639014 1105 54-55 0.27806467029761706 1105 56-57 0.22946731741125603 1105 58-59 0.1564926702334546 1105 60-61 0.07530701193729072 1105 62-63 0.026049639842938177 1105 64-65 -0.015244683117998648 1105 66-67 -0.01670038028724008 1105 68-69 0.1349656519629363 1105 70-71 0.21365088205276805 1105 72-73 0.09359156737745877 1105 74-75 0.12294095660281101 1105 76-77 0.27344292075418153 1105 78-79 0.25620352946453906 1105 80-81 0.3824871786836823 1105 82-83 0.3942125175320541 1105 84-85 0.4002811565289761 1105 86-87 0.32643104336764495 1105 88-89 0.5310719841339129 1105 90-91 0.5330954823626364 1105 92-93 0.49882230846577613 1105 94-95 0.45091726717972946 1105 96-97 0.44416987766410543 1105 98-99 0.7037910163664911 1105 100-101 0.53043074073166 1106 1 0.12046088380756714 1106 2 0.08429620545757643 1106 3 0.11714526865561936 1106 4 0.07851813617431702 1106 5 0.090033865010966 1106 6 0.06569627822883461 1106 7 0.0569544618960478 1106 8 -0.007295163497232693 1106 9 0.09242974182895836 1106 10-11 0.13530382040279676 1106 12-13 0.108238562599972 1106 14-15 0.11732834841409812 1106 16-17 0.1571913801765561 1106 18-19 0.1266594525236613 1106 20-21 0.21205912371229374 1106 22-23 0.10085965515928308 1106 24-25 0.11595505276605422 1106 26-27 0.1468925228279616 1106 28-29 0.16961561549414483 1106 30-31 0.17410116214348648 1106 32-33 0.11357381605132844 1106 34-35 0.0854915534058378 1106 36-37 0.09927633344472042 1106 38-39 0.26236040503729896 1106 40-41 0.34678720144341213 1106 42-43 0.33406920901819603 1106 44-45 0.2629089684991328 1106 46-47 0.3914565369579037 1106 48-49 0.2501794961495136 1106 50-51 0.23200849460096862 1106 52-53 0.24044209924915805 1106 54-55 0.19378294852297273 1106 56-57 0.33786116422630386 1106 58-59 0.3720063565569802 1106 60-61 0.3449142667709495 1106 62-63 0.4444091716267451 1106 64-65 0.3887957400542206 1106 66-67 0.47231955011844917 1106 68-69 0.368821754740317 1106 70-71 0.37669308201663654 1106 72-73 0.3352106087157978 1106 74-75 0.37280789954832727 1106 76-77 0.40386758928241306 1106 78-79 0.5831406826272847 1106 80-81 0.641209152546125 1106 82-83 0.462499068102602 1106 84-85 0.5363169499527949 1106 86-87 0.4090241329412585 1106 88-89 0.6601221745807031 1106 90-91 0.556348913736624 1106 92-93 0.5772289435660802 1106 94-95 0.8399007629393829 1106 96-97 0.8891795622891827 1106 98-99 1.189766518716059 1106 100-101 0.8850907681196318 1107 1 0.050581880718524985 1107 2 0.0711907628763555 1107 3 0.08083077583639664 1107 4 0.08454087318787629 1107 5 0.10964585318401987 1107 6 0.132251128409834 1107 7 0.13162147873533314 1107 8 0.08007008760655054 1107 9 0.11426181117507639 1107 10-11 0.1632461699185157 1107 12-13 0.15584885827200168 1107 14-15 0.21198032374748976 1107 16-17 0.047393941443921506 1107 18-19 0.09486031299993414 1107 20-21 0.15362290644544174 1107 22-23 0.12790220030411348 1107 24-25 0.09539998604838473 1107 26-27 0.13675053213970045 1107 28-29 0.1891135322820574 1107 30-31 0.1510057687870443 1107 32-33 0.1395863198005074 1107 34-35 0.29267229736017697 1107 36-37 0.379427696887916 1107 38-39 0.32098845456086167 1107 40-41 0.41462626661515145 1107 42-43 0.28340128623987937 1107 44-45 0.2664458446443021 1107 46-47 0.18512668595204929 1107 48-49 0.3294845593439035 1107 50-51 0.24360514415195667 1107 52-53 0.40566380643686983 1107 54-55 0.33669333732567 1107 56-57 0.4397080097960284 1107 58-59 0.4639346288679249 1107 60-61 0.28352951974000007 1107 62-63 0.25994098552259004 1107 64-65 0.3253097085761567 1107 66-67 0.38215266931923253 1107 68-69 0.4142442176177852 1107 70-71 0.3374077591407385 1107 72-73 0.436651986883831 1107 74-75 0.5250180906659736 1107 76-77 0.43995352482776795 1107 78-79 0.8234301487151718 1107 80-81 0.7010001333129949 1107 82-83 0.7024064447906895 1107 84-85 0.7698582977625854 1107 86-87 0.7512799874312641 1107 88-89 0.907008465140084 1107 90-91 1.0343495507457234 1107 92-93 0.9866613207059771 1107 94-95 0.9462334491497266 1107 96-97 0.7542820250870683 1107 98-99 0.9533197495144741 1107 100-101 0.9254080391076478 1108 1 0.024418669768984103 1108 2 -4.534959611177669E-4 1108 3 0.09276566148993481 1108 4 0.013154153596417473 1108 5 0.08926147825282982 1108 6 0.013452773980304755 1108 7 0.00389541624233658 1108 8 -0.01421081782101652 1108 9 0.012269089291613966 1108 10-11 0.022127827284087687 1108 12-13 0.029925091803612247 1108 14-15 0.0566513676239353 1108 16-17 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-0.4832473311636605 2316 60-61 -0.46984050771173 2316 62-63 -0.5360047195759705 2316 64-65 -0.5468870551593739 2316 66-67 -0.4622538478061884 2316 68-69 -0.34655348222029403 2316 70-71 -0.2894120503867086 2316 72-73 -0.34932339712531757 2316 74-75 -0.3164606563527599 2316 76-77 -0.47845893284661045 2316 78-79 -0.43638326370542657 2316 80-81 -0.4437495040094994 2316 82-83 -0.3770156916989329 2316 84-85 -0.5552365359830809 2316 86-87 -0.6551260440055522 2316 88-89 -0.5803590628955888 2316 90-91 -0.8948849706444371 2316 92-93 -0.7135761873778002 2316 94-95 -0.5298109005227651 2316 96-97 -0.5796267724443389 2316 98-99 -0.5018811703808055 2316 100-101 0.14050234850029142 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 18.0 5 52.0 6 109.0 7 233.0 8 624.0 9 1176.0 10 1898.0 11 3082.0 12 4377.0 13 5979.0 14 7539.0 15 9754.0 16 12703.0 17 17566.0 18 17131.0 19 12419.0 20 12118.0 21 12764.0 22 13777.0 23 15317.0 24 17824.0 25 21436.0 26 25624.0 27 31830.0 28 38487.0 29 47526.0 30 57834.0 31 70919.0 32 88495.0 33 113304.0 34 152712.0 35 218009.0 36 348589.0 37 591225.0 38 514965.0 39 43270.0 40 3.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.74185192067601 19.59592325616502 15.51953601542389 23.142688807735084 2 15.428072637839769 20.61412451614025 39.87162404591314 24.086178800106847 3 19.30096590181658 24.18611194092367 29.715824762555066 26.79709739470469 4 13.378593681095921 17.295664585022593 36.84284772702486 32.482894006856625 5 14.307746655855395 37.01521164961994 33.89898889315649 14.778052801368164 6 31.927275227487183 36.20073086728847 17.51465607035546 14.357337834868883 7 28.815399108228107 32.30337789031616 20.475664363739245 18.40555863771648 8 25.926070813832737 35.4592735899904 19.718480162738096 18.89617543343876 9 26.065597868092993 17.43076597231113 20.276035171407663 36.227600988188215 10-11 25.00440590952988 26.713958079450208 27.11685183341157 21.164784177608336 12-13 25.78209833515892 23.842628814569295 27.65913433954033 22.71613851073145 14-15 22.73044925223417 25.48706581362767 25.919644390141066 25.862840543997095 16-17 22.344828730781593 28.67105455273969 26.488261354312154 22.495855362166566 18-19 22.28623633377748 27.4123243760995 28.56380784386247 21.73763144626055 20-21 23.15435857069924 26.474833760884376 28.47912744814462 21.891680220271766 22-23 22.76995778229828 26.352080774744614 28.391246346849 22.4867150961081 24-25 22.61012454501072 26.600674163563596 28.46943216903364 22.319769122392042 26-27 22.49462344346589 26.74432090411108 28.46770361516006 22.29335203726297 28-29 22.35096171323891 26.949703875461733 28.515303324150075 22.184031087149286 30-31 22.573055116940345 26.642969590926118 28.32438716366907 22.459588128464468 32-33 22.398853752254325 26.645004607435435 28.340015300162957 22.61612634014728 34-35 22.868119872165334 26.788731976942344 28.09598466774348 22.247163483148842 36-37 22.844180484356748 26.427976185888713 28.33823515753125 22.389608172223284 38-39 22.54547387314618 26.733468509819524 28.345865018203774 22.375192598830516 40-41 22.752589011056763 26.629816720524737 28.19876522644003 22.418829041978466 42-43 22.642153261668067 26.62057938931328 27.919658921480256 22.8176084275384 44-45 22.67154836143746 26.800737430692994 27.895878516374168 22.631835691495382 46-47 22.816377029040684 26.982610678269296 27.53599708538103 22.66501520730899 48-49 22.885084395888555 26.824994902580006 27.643466688162764 22.646454013368675 50-51 22.80103623830952 27.03564874745722 27.527036083411176 22.636278930822083 52-53 22.961604968127293 27.119420300850518 27.222692449337966 22.69628228168422 54-55 22.784360957398214 26.923643809677355 27.358663164067377 22.933332068857055 56-57 22.77904620554378 27.106894082725198 27.348310264544246 22.765749447186778 58-59 22.933346557713058 26.84462809296871 27.4942559893499 22.72776935996834 60-61 22.93288738721028 26.901453559649408 27.65892708571456 22.506731967425754 62-63 22.727949878588742 26.89621856792016 27.565551211050103 22.81028034244099 64-65 22.52646706908624 26.848407471158087 27.545667351064452 23.07945810869122 66-67 22.55871121416593 26.916195254104412 27.38905010961429 23.136043422115375 68-69 22.51410688036417 26.898205225472992 27.50086933156306 23.086818562599774 70-71 22.652464751234987 26.798762314568965 27.412151975062944 23.136620959133104 72-73 22.713945434687428 26.729210824549178 27.382846272877142 23.173997467886252 74-75 22.502027113532584 27.107348503887472 27.111793928301033 23.27883045427891 76-77 22.588688080042413 26.851625391150808 27.272912708784574 23.28677382002221 78-79 22.624503495486607 26.899776819937 27.30008234901758 23.17563733555881 80-81 22.499083739764313 27.06133855988588 27.181840364251723 23.257737336098085 82-83 22.66670539125267 26.940457392681527 27.201275382385532 23.191561833680275 84-85 22.604726296206728 26.84761717348457 27.126987401074487 23.420669129234216 86-87 22.52683831426233 26.867586310017096 27.197015516954252 23.408559858766324 88-89 22.510496392667505 26.796145806707738 27.106422207913944 23.586935592710816 90-91 22.434362903737547 26.85223523066184 27.282227615965482 23.431174249635127 92-93 22.50710477608496 26.74489823336858 27.204515602846968 23.54348138769949 94-95 22.370284491356514 26.67954061576834 27.219768347945937 23.730406544929213 96-97 22.346575561150864 26.59572954749136 27.284809846476776 23.772885044881004 98-99 22.298723037019126 26.637872170931903 27.030768659323222 24.032636132725752 100-101 22.2614957579642 26.209658378852986 27.584069679199313 23.944776183983496 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 237.0 1 158.5 2 117.0 3 149.5 4 144.5 5 168.5 6 296.0 7 698.5 8 1190.5 9 1432.0 10 1383.0 11 1313.5 12 1488.0 13 1690.0 14 1841.0 15 2037.0 16 2215.0 17 2400.0 18 2603.5 19 2698.5 20 2976.5 21 3588.0 22 4317.5 23 5287.0 24 6602.5 25 8323.5 26 10866.5 27 14026.0 28 16852.5 29 20642.5 30 25414.0 31 30492.5 32 35781.0 33 40342.0 34 45330.0 35 50417.5 36 56216.0 37 62150.0 38 66533.0 39 70503.0 40 74422.5 41 79399.5 42 84330.0 43 88451.5 44 92821.5 45 96909.0 46 99156.0 47 103357.0 48 111472.5 49 110447.0 50 102424.0 51 97177.5 52 93001.0 53 90439.0 54 87909.0 55 81865.0 56 73055.5 57 65118.5 58 58211.5 59 50598.0 60 42460.0 61 34616.5 62 27445.5 63 21154.5 64 15670.0 65 11325.0 66 8483.5 67 6930.0 68 5448.0 69 4007.5 70 3026.5 71 2301.0 72 1807.0 73 1453.5 74 1086.0 75 832.5 76 571.0 77 279.0 78 157.0 79 95.5 80 56.0 81 46.0 82 30.0 83 14.0 84 8.5 85 7.0 86 5.0 87 2.5 88 0.5 89 0.0 90 1.0 91 1.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26929393225252224 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0021140462766705706 16-17 4.939360459510679E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.9757441838042716E-5 26-27 0.0011656890684445204 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.003141433252248792 36-37 0.0202118630003177 38-39 0.00683607487596278 40-41 0.017011157422554782 42-43 0.005176449761567192 44-45 5.334509296271533E-4 46-47 3.951488367608543E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 4.939360459510679E-4 60-61 0.001679382556233631 62-63 0.001462050696015161 64-65 0.0 66-67 0.0 68-69 4.7417860411302524E-4 70-71 1.9757441838042716E-5 72-73 0.0 74-75 0.0 76-77 0.23434301764102466 78-79 0.0 80-81 9.681146500640931E-4 82-83 0.0 84-85 0.0 86-87 0.10601843290293722 88-89 0.3647421337721066 90-91 0.3655917037711425 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 3.358765112467262E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2530692.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.22357285374558 #Duplication Level Percentage of deduplicated Percentage of total 1 73.66187920467652 39.94210273598585 2 14.430841739269345 15.649835967802883 3 5.136140651296609 8.354996903779979 4 2.294824492642377 4.977343322534148 5 1.2343077504361901 3.346428811485979 6 0.7520460153034451 2.4467173140105256 7 0.5053469016802541 1.918120017777171 8 0.3444474276804332 1.4941736151292186 9 0.24048613492986282 1.1736015711916599 >10 1.2746858752677754 12.563779403403103 >50 0.08552862184939311 3.132694611659481 >100 0.037204380360808635 3.5149027548197385 >500 0.0016044419790898047 0.5732415298006532 >1k 5.83433446935966E-4 0.6789448002946609 >5k 7.292918086699574E-5 0.23311664032483115 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5895 0.23294023927052362 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4749 0.18765618257772973 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3252 0.12850240171462984 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2904 0.1147512221953521 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 3.951488367608543E-5 0.0 6 0.0 0.0 0.0 3.951488367608543E-5 0.0 7 0.0 0.0 0.0 3.951488367608543E-5 0.0 8 0.0 0.0 0.0 3.951488367608543E-5 0.0 9 0.0 0.0 0.0 3.951488367608543E-5 0.0 10-11 0.0 0.0 0.0 3.951488367608543E-5 0.0 12-13 0.0 0.0 0.0 5.927232551412815E-5 0.0 14-15 0.0 0.0 0.0 7.902976735217086E-5 0.0 16-17 0.0 0.0 0.0 7.902976735217086E-5 0.0 18-19 0.0 0.0 0.0 9.878720919021357E-5 0.0 20-21 0.0 0.0 0.0 1.3830209286629902E-4 0.0 22-23 0.0 0.0 0.0 1.9757441838042718E-4 0.0 24-25 0.0 0.0 0.0 2.7660418573259804E-4 0.0 26-27 0.0 0.0 0.0 6.124806969793242E-4 0.0 28-29 0.0 0.0 0.0 0.0023313781368890403 0.0 30-31 0.0 0.0 0.0 0.006895347201476908 0.0 32-33 0.0 0.0 0.0 0.016457949051089583 0.0 34-35 0.0 0.0 0.0 0.03084136670918468 0.0 36-37 0.0 0.0 0.0 0.05407611831072291 0.0 38-39 3.951488367608543E-5 0.0 0.0 0.08610293153019016 0.0 40-41 3.951488367608543E-5 0.0 0.0 0.12356304125511916 0.0 42-43 5.927232551412815E-5 0.0 0.0 0.16564639237015016 0.0 44-45 7.902976735217086E-5 0.0 0.0 0.2159488392898069 0.0 46-47 1.3830209286629902E-4 0.0 0.0 0.27982464875219903 0.0 48-49 1.5805953470434173E-4 0.0 0.0 0.3494103589057854 0.0 50-51 1.5805953470434173E-4 0.0 0.0 0.42567408440063037 0.0 52-53 1.5805953470434173E-4 0.0 0.0 0.5008511505943829 0.0 54-55 1.5805953470434173E-4 0.0 0.0 0.5926047104902532 0.0 56-57 1.5805953470434173E-4 0.0 0.0 0.7002827685075861 0.0 58-59 1.5805953470434173E-4 0.0 0.0 0.8215341890676542 0.0 60-61 1.5805953470434173E-4 0.0 0.0 0.9534743856621035 0.0 62-63 1.5805953470434173E-4 0.0 0.0 1.0905317596926059 0.0 64-65 1.5805953470434173E-4 0.0 0.0 1.2371122206890446 0.0 66-67 1.5805953470434173E-4 0.0 0.0 1.409318083749425 0.0 68-69 1.5805953470434173E-4 0.0 0.0 1.606734442595148 0.0 70-71 1.5805953470434173E-4 0.0 0.0 1.824619511185083 0.0 72-73 1.5805953470434173E-4 0.0 0.0 2.049834590696932 0.0 74-75 1.5805953470434173E-4 0.0 0.0 2.29676309878879 0.0 76-77 1.5805953470434173E-4 0.0 0.0 2.5676178689465177 0.0 78-79 1.5805953470434173E-4 0.0 0.0 2.8719417455778893 0.0 80-81 1.9757441838042718E-4 0.0 0.0 3.2084109800797567 0.0 82-83 1.9757441838042718E-4 0.0 0.0 3.5594612066581 0.0 84-85 2.1733186021846988E-4 0.0 0.0 3.9193430097380477 0.0 86-87 2.370893020565126E-4 0.0 0.0 4.308347282087271 0.0 88-89 2.370893020565126E-4 0.0 0.0 4.733507673000112 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8380 0.0 26.288914 1 GTATCAA 11460 0.0 26.28178 1 GTACATG 8920 0.0 22.137009 1 ATCAACG 13380 0.0 22.131163 3 TCAACGC 13495 0.0 21.977732 4 CAACGCA 13610 0.0 21.792027 5 TATCAAC 13865 0.0 21.52814 2 TACATGG 9040 0.0 21.31245 2 AACGCAG 14055 0.0 21.135826 6 AGTACTT 7005 0.0 20.120214 12-13 GAGTACT 6795 0.0 20.00873 12-13 ACATGGG 9330 0.0 19.836212 3 GTACTTT 7090 0.0 18.97541 14-15 ACGCAGA 15580 0.0 18.945177 7 CGCAGAG 15675 0.0 18.739538 8 AGAGTAC 12625 0.0 18.662603 10-11 CATGGGG 4665 0.0 17.700005 4 GCAGAGT 16940 0.0 17.004333 9 TGCACCG 450 7.2759576E-12 16.872646 5 TACTTTT 8655 0.0 16.25706 14-15 >>END_MODULE