FastQCFastQC Report
Fri 27 May 2016
SRR939273_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939273_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences380581
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17570.4616625632913887No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13950.3665448353964071No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12680.3331748037868417No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9290.2441004674431987No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT7960.20915389890719716No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT6250.1642225965037666No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC4960.13032705258538918No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT4080.10720451099765883No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCTT300.003933398531.69410784-85
CTCCGAA600.00398198431.6107032
GTGTCGC650.005901274329.1675979
TGTTCGC650.005901274329.1675978
GCGGCGT350.00835980627.16279640-41
ACTATAC909.029249E-426.342253
GTACATG21900.025.1418191
CTATAGG1151.2857762E-424.764881
GAGTACT15950.024.21870412-13
TACATGG21750.024.1985362
GTACTTT16650.023.05968514-15
ACATGGG21500.022.2745173
GTATATG1503.0430823E-522.1508061
GCACCGT1503.077303E-522.118766
AGTACTT16150.021.8644112-13
GTATCAA40050.021.6885931
ACGGACC550.002862051421.6096268-69
ATGGGGA4400.021.5499155
GACCATA1100.002910167721.5442477
TAGGACA1805.239268E-621.0710284
Produced by FastQC (version 0.11.5)