##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939273_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 380581 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.948541834721123 33.0 31.0 34.0 30.0 34.0 2 32.00170265988055 33.0 31.0 34.0 30.0 34.0 3 32.05050436043838 34.0 31.0 34.0 30.0 34.0 4 35.52782719053237 37.0 35.0 37.0 33.0 37.0 5 35.57717279632982 37.0 35.0 37.0 33.0 37.0 6 35.6773590904433 37.0 35.0 37.0 35.0 37.0 7 35.61651002020595 37.0 35.0 37.0 33.0 37.0 8 35.61340949758396 37.0 35.0 37.0 33.0 37.0 9 37.2298117877666 39.0 37.0 39.0 34.0 39.0 10-11 37.140218508018 39.0 37.0 39.0 34.0 39.0 12-13 37.05039400285354 39.0 37.0 39.0 33.0 39.0 14-15 38.271645195109585 40.0 38.0 41.0 33.0 41.0 16-17 38.213817820647904 40.0 38.0 41.0 33.0 41.0 18-19 38.173826333947304 40.0 38.0 41.0 33.0 41.0 20-21 38.15849582611848 40.0 38.0 41.0 33.5 41.0 22-23 38.159052869165826 40.0 38.0 41.0 33.0 41.0 24-25 38.07571581345364 40.0 38.0 41.0 33.0 41.0 26-27 37.993743775963594 40.0 38.0 41.0 33.0 41.0 28-29 37.84301108042703 40.0 38.0 41.0 33.0 41.0 30-31 37.65322493766111 40.0 37.5 41.0 32.5 41.0 32-33 37.41989878632933 40.0 37.0 41.0 32.0 41.0 34-35 37.135406129050054 40.0 36.5 41.0 31.0 41.0 36-37 36.89401073621647 40.0 36.0 41.0 30.5 41.0 38-39 36.32091066027994 39.5 35.5 40.5 29.5 41.0 40-41 36.29544302001413 39.0 35.0 40.5 30.0 41.0 42-43 36.4425917216046 39.5 36.0 41.0 30.0 41.0 44-45 36.29996373965069 40.0 36.0 41.0 30.0 41.0 46-47 35.96337441963735 40.0 35.5 41.0 28.5 41.0 48-49 35.67449242079873 39.0 35.0 41.0 27.0 41.0 50-51 34.5028982003831 38.0 34.0 40.0 25.0 40.5 52-53 34.60269692916882 38.5 34.5 39.5 24.5 40.5 54-55 35.08858955123876 39.0 35.0 40.5 26.0 41.0 56-57 35.007033982253446 39.0 35.0 41.0 25.5 41.0 58-59 34.800623257598254 38.5 35.0 40.5 25.0 41.0 60-61 34.48880001891844 38.0 34.0 40.0 23.5 41.0 62-63 34.12723704020957 38.0 34.0 40.0 22.5 41.0 64-65 33.73795985611473 37.0 34.0 40.0 22.0 41.0 66-67 33.364942022854535 36.5 33.5 39.5 21.5 41.0 68-69 32.955705881271 36.0 33.0 39.0 20.0 41.0 70-71 32.52696535034592 35.5 32.5 39.0 18.5 40.0 72-73 32.08864604381196 35.0 32.0 38.0 17.5 40.0 74-75 31.635010681037677 35.0 32.0 37.0 13.5 39.0 76-77 31.176662786634118 35.0 31.0 37.0 9.0 39.0 78-79 30.79497137271698 35.0 31.0 36.0 7.0 39.0 80-81 30.401391556593737 35.0 31.0 36.0 3.5 37.5 82-83 29.975804099521522 34.0 30.5 35.0 2.0 37.0 84-85 29.5787848053371 34.0 30.0 35.0 2.0 36.5 86-87 29.190031819770297 34.0 29.5 35.0 2.0 36.0 88-89 28.760755266290225 34.0 29.0 35.0 2.0 36.0 90-91 28.348589393585073 34.0 29.0 35.0 2.0 35.5 92-93 27.916138745759774 34.0 28.5 35.0 2.0 35.0 94-95 27.459272796067065 34.0 27.0 35.0 2.0 35.0 96-97 26.88318518265494 33.5 25.5 35.0 2.0 35.0 98-99 26.15039505387815 33.0 24.5 35.0 2.0 35.0 100-101 23.515230660490147 30.0 10.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15152382378184015 1101 2 0.28823857257183505 1101 3 0.21934087592377693 1101 4 0.10575955236154044 1101 5 0.0492294530174604 1101 6 0.2350086387969199 1101 7 0.2273475283702524 1101 8 0.19582621972292458 1101 9 0.3504107136095058 1101 10-11 0.340177097142373 1101 12-13 0.3156435759099523 1101 14-15 0.4787784729684006 1101 16-17 0.598320134466725 1101 18-19 0.5053008782511483 1101 20-21 0.5156517947304877 1101 22-23 0.5484540788521528 1101 24-25 0.5906135451729142 1101 26-27 0.36528166506750637 1101 28-29 0.4150898571537809 1101 30-31 0.5245058789571857 1101 32-33 0.5678690684247911 1101 34-35 0.5667903194517336 1101 36-37 0.7060146503901379 1101 38-39 1.0635424901706756 1101 40-41 0.7853036685986297 1101 42-43 0.6840875144791028 1101 44-45 0.6193845935668989 1101 46-47 0.5102886461057992 1101 48-49 0.37755921480156474 1101 50-51 0.6853442555672089 1101 52-53 0.6175139655363111 1101 54-55 0.3746545919106623 1101 56-57 0.2657981894515089 1101 58-59 0.16506214320077817 1101 60-61 0.16697206438635703 1101 62-63 0.4014403483389728 1101 64-65 0.247229554913325 1101 66-67 0.4000163124630163 1101 68-69 0.3147592772352539 1101 70-71 0.294791399269009 1101 72-73 0.4753173533613264 1101 74-75 0.0852186293550865 1101 76-77 0.30959505358858763 1101 78-79 -0.0493209856231438 1101 80-81 -0.3126291240506127 1101 82-83 -0.03675598910571409 1101 84-85 -0.3062535189560087 1101 86-87 -0.653932448507522 1101 88-89 -0.727466860226432 1101 90-91 -0.23116357830079082 1101 92-93 -0.0210830088470928 1101 94-95 -0.30699958452919773 1101 96-97 -0.31866592623856604 1101 98-99 -0.13855424041305042 1101 100-101 0.3655015888605355 1102 1 0.11345117922129688 1102 2 0.11086536291906413 1102 3 0.17797186003722487 1102 4 0.16619598837106508 1102 5 0.24222266480265375 1102 6 0.13331869317372025 1102 7 0.10963775120850983 1102 8 0.09022683101765949 1102 9 -0.022097016425682625 1102 10-11 0.09973301994864414 1102 12-13 0.12472947703657411 1102 14-15 0.28272878750086505 1102 16-17 0.3239260981444474 1102 18-19 0.5034305651402988 1102 20-21 0.42095442116542614 1102 22-23 0.3321907956307655 1102 24-25 0.30288741943100916 1102 26-27 0.17703753811722578 1102 28-29 0.3324540040066708 1102 30-31 0.44031957622412676 1102 32-33 0.4247701239744188 1102 34-35 0.4258104708537971 1102 36-37 0.42571202395519947 1102 38-39 0.7041758199499668 1102 40-41 0.6466872560457588 1102 42-43 0.6477519065606998 1102 44-45 1.0137807623126776 1102 46-47 1.1236713805835237 1102 48-49 1.0099427311679108 1102 50-51 0.7671404669614574 1102 52-53 0.9835044762517455 1102 54-55 1.1753218627639868 1102 56-57 1.0233727354230027 1102 58-59 0.8979139042271811 1102 60-61 0.8216927169079469 1102 62-63 0.9911131990337907 1102 64-65 0.700605003610768 1102 66-67 0.6157020643200042 1102 68-69 0.6456447660933406 1102 70-71 0.5332363294321354 1102 72-73 0.8519477688285022 1102 74-75 0.6506351627452851 1102 76-77 0.4772390810257683 1102 78-79 0.41699560818572223 1102 80-81 0.4605387687595659 1102 82-83 0.2464609494449519 1102 84-85 0.20030368330436232 1102 86-87 0.298510064908978 1102 88-89 0.3595671163879892 1102 90-91 0.46074120898996185 1102 92-93 0.29056180047870583 1102 94-95 0.10778788392870453 1102 96-97 0.13467398206722336 1102 98-99 -0.09123665147321702 1102 100-101 -0.4364540211902863 1103 1 0.527752877929462 1103 2 0.25126308441229384 1103 3 0.4014486172338465 1103 4 0.5358938827866879 1103 5 0.4904425813930331 1103 6 0.4827236367196406 1103 7 0.3638590918656135 1103 8 0.4738348057913342 1103 9 0.6096545856493876 1103 10-11 0.6605101535057543 1103 12-13 0.7072617728355794 1103 14-15 0.7518189105809228 1103 16-17 0.6765987662351804 1103 18-19 0.7718671433113897 1103 20-21 0.7967936033442484 1103 22-23 0.696027129676132 1103 24-25 0.8276305788247669 1103 26-27 0.8467939209877429 1103 28-29 0.7395082202613779 1103 30-31 0.8132470505400704 1103 32-33 0.48446649260426256 1103 34-35 0.5463984069742338 1103 36-37 0.6030337613166026 1103 38-39 0.8510199863623384 1103 40-41 1.035978782848737 1103 42-43 0.8938230081780318 1103 44-45 0.8175392812832811 1103 46-47 1.0098731911826562 1103 48-49 1.1866922988619493 1103 50-51 1.0429643912824815 1103 52-53 1.3016816708402885 1103 54-55 1.0939974807072232 1103 56-57 1.2169822359824636 1103 58-59 1.219244389427054 1103 60-61 1.1898393957809006 1103 62-63 1.0282406530059163 1103 64-65 1.0699341664629642 1103 66-67 1.1401146367948272 1103 68-69 0.7893992163018666 1103 70-71 1.0358937396650774 1103 72-73 1.116468163498432 1103 74-75 1.0617454261976285 1103 76-77 1.2064756795406701 1103 78-79 1.5329044679151558 1103 80-81 1.353899057641673 1103 82-83 1.158715552232053 1103 84-85 1.512123437144254 1103 86-87 1.3850856930241235 1103 88-89 1.3756005819560926 1103 90-91 1.5840628446323208 1103 92-93 1.594828914709204 1103 94-95 1.8156634815281336 1103 96-97 1.70285117665577 1103 98-99 1.5160642334825347 1103 100-101 1.4454247296997558 1104 1 0.1353201990847488 1104 2 0.029347484138781965 1104 3 0.2729604533837602 1104 4 0.2940424289102239 1104 5 -0.14262863087618882 1104 6 -0.1627779750806866 1104 7 0.06669358113656187 1104 8 0.11822118200832676 1104 9 0.1808912760860366 1104 10-11 0.1162914696465549 1104 12-13 0.20612618623794532 1104 14-15 0.09667498820783038 1104 16-17 0.27662400149291244 1104 18-19 0.2761164350960925 1104 20-21 0.341976925281017 1104 22-23 0.5316275660018803 1104 24-25 0.2630853018881609 1104 26-27 0.20113357127591058 1104 28-29 0.28749591196793034 1104 30-31 0.49301163000670556 1104 32-33 0.3284027043850628 1104 34-35 0.3801713222163343 1104 36-37 0.47636090794159003 1104 38-39 0.9233841229909103 1104 40-41 0.6508195565128929 1104 42-43 0.30319994152439733 1104 44-45 0.49077525536422684 1104 46-47 0.36810802560680145 1104 48-49 0.4105168242762929 1104 50-51 0.3119356068078645 1104 52-53 0.31783223578444364 1104 54-55 0.4335175223861043 1104 56-57 0.46396621566400853 1104 58-59 0.35007699012387405 1104 60-61 0.6382210831241935 1104 62-63 0.41616354708296655 1104 64-65 0.4004387088093537 1104 66-67 0.7395415522322892 1104 68-69 0.5739959406026003 1104 70-71 0.8316143283792172 1104 72-73 0.6184707033050145 1104 74-75 0.6872826929750069 1104 76-77 0.6835156652135588 1104 78-79 0.40887724639842915 1104 80-81 0.6400953716659608 1104 82-83 0.10639309448075807 1104 84-85 0.07382771248533615 1104 86-87 -0.23003999484015836 1104 88-89 -0.48238597009366657 1104 90-91 -0.11312627327331981 1104 92-93 -0.3456077366534984 1104 94-95 -0.21680452667739658 1104 96-97 0.089398342166497 1104 98-99 0.2796383600169996 1104 100-101 0.12870596443649518 1105 1 0.1973482172461054 1105 2 0.10456965030743248 1105 3 0.18497876996570284 1105 4 0.3378935678756534 1105 5 0.04493006367928842 1105 6 0.1733235422463295 1105 7 0.3894534864491419 1105 8 0.38669104890265515 1105 9 0.4293758893810846 1105 10-11 0.42497244290900227 1105 12-13 0.2654455234260382 1105 14-15 0.4986579910406874 1105 16-17 0.31646256721140986 1105 18-19 0.3787164583798841 1105 20-21 0.45522928276481167 1105 22-23 0.7291439616710917 1105 24-25 0.38717238326501047 1105 26-27 0.379611587496953 1105 28-29 0.21374062460715493 1105 30-31 0.3347633471862679 1105 32-33 0.3704726333695021 1105 34-35 0.1600812915593366 1105 36-37 -0.1250011938085649 1105 38-39 0.32705132001058246 1105 40-41 0.10401137614112343 1105 42-43 0.0383002558555674 1105 44-45 -0.06295985271136573 1105 46-47 -0.24806256696566464 1105 48-49 -0.026364700035870214 1105 50-51 -0.3060239570091241 1105 52-53 -0.1512391290337476 1105 54-55 -0.12355880964075538 1105 56-57 -0.45690770260988955 1105 58-59 -0.27822057672000255 1105 60-61 -0.687290190111085 1105 62-63 -0.5844030251549981 1105 64-65 -0.36767802059419097 1105 66-67 -0.28418074975846963 1105 68-69 -0.4728665686139024 1105 70-71 -0.43006831355489084 1105 72-73 -0.36270047136222416 1105 74-75 -0.4123098406894705 1105 76-77 -0.33915886620015456 1105 78-79 -0.3932803848639388 1105 80-81 -0.44475076128448165 1105 82-83 -0.055797910214806734 1105 84-85 -0.6493629429533669 1105 86-87 -0.6016919797833076 1105 88-89 -0.46126369138669077 1105 90-91 -0.10735189320424254 1105 92-93 -0.08493017834700822 1105 94-95 -0.6902765279191989 1105 96-97 -1.2466449656840197 1105 98-99 -0.8988901044170099 1105 100-101 -0.5546461252837105 1106 1 0.28095891953676144 1106 2 0.43030269683748656 1106 3 0.34065256897698504 1106 4 0.2463052060506783 1106 5 0.31572518802359895 1106 6 0.2772519923765273 1106 7 0.42529634844393627 1106 8 0.4088352807604636 1106 9 0.3808382087138895 1106 10-11 0.667872174974363 1106 12-13 0.761800102844127 1106 14-15 0.657335676991103 1106 16-17 0.5937522762570282 1106 18-19 0.49225281824251965 1106 20-21 0.6242016968181758 1106 22-23 0.5125584301416026 1106 24-25 0.08974229156343227 1106 26-27 0.4480217161810529 1106 28-29 0.45801776714083786 1106 30-31 0.27432333058186487 1106 32-33 0.49473769997579353 1106 34-35 0.44162021755654735 1106 36-37 0.4635115802583769 1106 38-39 0.8431236246258536 1106 40-41 0.7993847864584964 1106 42-43 0.7892229321829802 1106 44-45 0.6879439549794881 1106 46-47 0.14450319023271874 1106 48-49 0.4388415341616536 1106 50-51 0.35657463161685854 1106 52-53 0.6311677542448635 1106 54-55 0.7139746821891251 1106 56-57 0.886558088552043 1106 58-59 0.7675962465563444 1106 60-61 0.6962752274518422 1106 62-63 0.5084344404097365 1106 64-65 0.8977697321511968 1106 66-67 0.7001507344484779 1106 68-69 1.0328921392618682 1106 70-71 1.1134918207897968 1106 72-73 0.7779576666807273 1106 74-75 0.9651723284489826 1106 76-77 1.2662149214899507 1106 78-79 0.9851225737382698 1106 80-81 0.9904883043406372 1106 82-83 1.0832073352661524 1106 84-85 1.0433764057012986 1106 86-87 1.4335206697506386 1106 88-89 1.141143946637385 1106 90-91 1.461960156307061 1106 92-93 1.4889820447557511 1106 94-95 1.9275543336227479 1106 96-97 2.0963868468372056 1106 98-99 1.6486399910582925 1106 100-101 1.5710303153366887 1107 1 0.29876815358145237 1107 2 0.10412614374425289 1107 3 0.21739051573629098 1107 4 0.26274781874299435 1107 5 -0.05608213275120022 1107 6 0.21109313343342961 1107 7 0.19598447825858045 1107 8 0.3596800685385517 1107 9 0.15410853361013466 1107 10-11 0.49924548557862636 1107 12-13 0.36964810577877216 1107 14-15 0.5472219597090984 1107 16-17 0.5477798532373797 1107 18-19 0.3906660989249673 1107 20-21 0.3533157665446325 1107 22-23 0.3768852971981147 1107 24-25 0.45689590699618776 1107 26-27 0.5362802809409217 1107 28-29 0.6274392547441394 1107 30-31 0.775905734253044 1107 32-33 0.6146659163910684 1107 34-35 0.5914450742411219 1107 36-37 0.5312096864088929 1107 38-39 1.015019416151361 1107 40-41 0.7951734348699873 1107 42-43 0.44622614733953014 1107 44-45 0.5631426545313829 1107 46-47 0.8628713004830999 1107 48-49 0.9362233753602496 1107 50-51 0.8461703274788732 1107 52-53 0.9541830963677356 1107 54-55 1.271423128196794 1107 56-57 1.0154089038495684 1107 58-59 0.825738792356141 1107 60-61 0.7562914830531895 1107 62-63 0.9317849644011389 1107 64-65 0.7557741772669644 1107 66-67 1.0022136134161812 1107 68-69 1.0276198579392641 1107 70-71 0.7127229164604856 1107 72-73 0.9192051761931239 1107 74-75 1.0100550507587194 1107 76-77 1.303775430448507 1107 78-79 1.209219704552705 1107 80-81 0.8831186188793332 1107 82-83 0.9686664840768344 1107 84-85 1.056434832360484 1107 86-87 1.0143922864636998 1107 88-89 0.9667630620737349 1107 90-91 1.0572891883795066 1107 92-93 0.9412545922098801 1107 94-95 0.8920905095284937 1107 96-97 1.0949087379534497 1107 98-99 1.2674785067636485 1107 100-101 0.7506151501553262 1108 1 -0.10234658011353659 1108 2 -0.02375031562343466 1108 3 -0.12940124281022136 1108 4 -0.1035856002697102 1108 5 -0.04479951258588244 1108 6 -0.04461243617654986 1108 7 0.039414448902121535 1108 8 -0.2594300384704695 1108 9 0.18197011182412837 1108 10-11 0.2298627828905495 1108 12-13 0.012581587021749385 1108 14-15 0.10150773197333507 1108 16-17 0.2785358158450961 1108 18-19 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-1.6017627717289926 2316 76-77 -1.6089576219322588 2316 78-79 -1.4865303775618983 2316 80-81 -1.1527946903670525 2316 82-83 -1.4169130780157246 2316 84-85 -1.2967999263339216 2316 86-87 -1.5327665482326474 2316 88-89 -1.393132721967639 2316 90-91 -1.3308790714032668 2316 92-93 -1.7441351320520653 2316 94-95 -1.4827612666521475 2316 96-97 -1.3683261877537092 2316 98-99 -1.5389286591969267 2316 100-101 -1.2012740424493522 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1690.0 3 337.0 4 337.0 5 398.0 6 427.0 7 479.0 8 582.0 9 731.0 10 870.0 11 1095.0 12 1493.0 13 1964.0 14 2160.0 15 2301.0 16 2987.0 17 4033.0 18 3486.0 19 2240.0 20 2185.0 21 2186.0 22 2356.0 23 2624.0 24 3064.0 25 3371.0 26 3876.0 27 4581.0 28 5580.0 29 6531.0 30 7897.0 31 9635.0 32 12102.0 33 15885.0 34 21903.0 35 32063.0 36 50461.0 37 81242.0 38 74771.0 39 10653.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.86020391760022 19.047242579723665 14.924881478494836 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29.897830845980593 20.98809122866104 20-21 22.833866491564432 26.209409464205585 29.620086928258527 21.336637115971456 22-23 22.273558102903074 26.039617380273423 29.74053813630137 21.946286380522135 24-25 22.285839891437544 26.483684700301545 29.68711952794812 21.543355880312788 26-27 22.056938621355798 26.322992580661293 30.127805281179516 21.492263516803394 28-29 21.380783311532277 27.211008787975295 29.971101536777113 21.437106363715323 30-31 21.838407417105607 26.420280720499278 29.780531676783255 21.96078018561186 32-33 21.824096518724982 26.63292511850337 29.62426338410357 21.918714978668078 34-35 22.523767272554686 26.618554227351048 29.227252172376833 21.63042632771743 36-37 22.23593515294391 26.278184472503884 29.68249652190212 21.803383852650086 38-39 21.873177400157815 26.82998920625035 29.62435639958514 21.672476994006697 40-41 21.994286958300073 26.658398369210268 29.771148479710785 21.576166192778874 42-43 21.74163783160323 26.936436170914725 28.956738167066533 22.365187830415515 44-45 21.895988544354335 26.86282920133825 28.924977398855756 22.31620485545166 46-47 22.328849024454946 27.09353169350081 28.453078414473676 22.124540867570566 48-49 22.423517640738496 27.015165134841528 28.36523040735593 22.196086817064042 50-51 22.084614196695735 27.31515640282091 28.46611855174116 22.134110848742193 52-53 22.446560522501734 27.530999780882965 27.81901988685113 22.20341980976417 54-55 21.706016655009037 27.23740646404392 28.275375133622795 22.781201747324246 56-57 22.089975184991207 27.495979599186814 28.000459097451348 22.413586118370628 58-59 22.613218448420508 26.869430095555686 28.203437049856745 22.31391440616706 60-61 22.30517387102176 27.06607402094126 28.68077430719917 21.94797780083781 62-63 22.014717043392178 27.14865533740307 28.658373205741626 22.178254413463126 64-65 21.70697647406657 27.226938672072986 28.443492355127553 22.622592498732892 66-67 21.911065291914774 27.449585317206264 27.908422621365503 22.730926769513456 68-69 21.811453670051183 27.272139189854634 28.2473606180583 22.669046522035885 70-71 22.111511973735798 27.265346856176564 28.040230590614534 22.582910579473108 72-73 22.059378704239034 27.000388078851284 28.264416337327642 22.675816879582037 74-75 21.69640087387548 27.746998528140242 27.852734821033735 22.703865776950543 76-77 22.017694817192268 27.22359766462198 27.906553205865897 22.852154312319858 78-79 21.9089455182679 27.275438355730557 28.033521941538492 22.78209418446305 80-81 22.15469307077014 27.613074675864475 27.782102810274168 22.45012944309122 82-83 22.170387751169905 27.3769587491455 27.909585432739387 22.54306806694521 84-85 21.813303701513966 27.39579594163728 27.91100952300513 22.879890833843618 86-87 21.919111669499667 27.372990065141423 27.964093035041227 22.743805230317683 88-89 21.992500801788616 27.229269309907465 27.59406663226345 23.18416325604047 90-91 22.02582810043469 27.23791395121359 27.888038629946394 22.848219318405327 92-93 22.36122992111016 27.129075182967398 27.78939475546262 22.72030014045982 94-95 22.050990274805716 26.97233197406791 27.754096607732897 23.22258114339348 96-97 21.951934271978203 26.865959294881105 28.022720732470376 23.159385700670324 98-99 21.925717461293853 27.043284620370212 27.49301381923975 23.537984099096185 100-101 22.147228494918124 26.434453004053093 28.142136722920764 23.276181778108022 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 519.0 1 296.5 2 77.0 3 94.0 4 96.5 5 87.5 6 136.5 7 280.5 8 461.0 9 568.5 10 551.0 11 523.5 12 576.0 13 621.0 14 631.5 15 661.0 16 715.5 17 775.5 18 835.5 19 863.0 20 876.0 21 946.5 22 1079.5 23 1212.5 24 1410.5 25 1724.0 26 2164.0 27 2739.0 28 3198.5 29 3719.0 30 4403.0 31 5105.0 32 5938.5 33 6657.0 34 7507.5 35 8284.0 36 9016.5 37 10009.0 38 10598.5 39 10715.0 40 10879.0 41 11380.0 42 11799.0 43 12297.0 44 12882.0 45 13281.0 46 13518.5 47 14600.0 48 17862.5 49 18022.5 50 15137.5 51 13751.0 52 12910.5 53 12647.5 54 12320.5 55 11267.0 56 9947.5 57 8778.5 58 7717.5 59 6604.0 60 5590.5 61 4662.0 62 3811.5 63 3002.5 64 2260.5 65 1741.5 66 1336.5 67 1091.5 68 806.0 69 510.5 70 369.0 71 294.5 72 270.5 73 220.0 74 163.0 75 146.0 76 103.5 77 57.0 78 36.0 79 26.0 80 15.5 81 13.5 82 10.5 83 8.0 84 6.0 85 3.5 86 1.5 87 0.5 88 0.0 89 1.0 90 1.5 91 1.0 92 1.0 93 0.5 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2927103560083136 2 0.18077623423134628 3 0.18025072192253425 4 0.19995743350298625 5 0.21151870429685138 6 0.1108830971593432 7 0.12691122257811083 8 0.13295461412944945 9 0.1413628110704423 10-11 0.12980154027657712 12-13 0.12021094064075714 14-15 0.13321737028385547 16-17 0.11206549985417033 18-19 0.13794698106316394 20-21 0.1609381445736913 22-23 0.16330294996334552 24-25 0.1858999792422638 26-27 0.1650108649669847 28-29 0.16553637727579673 30-31 0.1562085337943828 32-33 0.16658740189342086 34-35 0.34959706343721836 36-37 0.46823146715153935 38-39 0.4356497040051921 40-41 0.4265846166781841 42-43 0.44721097479905725 44-45 0.45417401289081694 46-47 0.45758984289809523 48-49 0.4688883575375545 50-51 0.464421502912652 52-53 0.4698080040779755 54-55 0.4508895609607416 56-57 0.4142350774211009 58-59 0.40333069701325075 60-61 0.3619466026943016 62-63 0.464684259067058 64-65 0.4637646125266369 66-67 0.4723041875448327 68-69 0.4851792391107281 70-71 0.4767710421697352 72-73 0.47085902869559965 74-75 0.4751945052432991 76-77 0.4501012924975235 78-79 0.4374889970860342 80-81 0.43354765476994384 82-83 0.4477364871078693 84-85 0.4490502678798994 86-87 0.4501012924975235 88-89 0.4573270867436892 90-91 0.4749317490888931 92-93 0.4795299817909985 94-95 0.47887309140498346 96-97 0.475063127166096 98-99 0.4723041875448327 100-101 0.4557505498172531 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 380581.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.73902681317043 #Duplication Level Percentage of deduplicated Percentage of total 1 85.60007110943016 57.98465512090984 2 8.833647594768856 11.967653825602918 3 2.2844223387176483 4.642336381650009 4 0.9593060701317907 2.599298384267781 5 0.5411449084832497 1.8328314732778752 6 0.3374667627264612 1.3715802053328947 7 0.2487899890385039 1.1796954216829316 8 0.1853350017279078 1.0043530121172588 9 0.14213369310565416 0.866519824350496 >10 0.7936357113482954 9.837440242597193 >50 0.05078603920682311 2.337232989047237 >100 0.020934703183177403 2.598530384885894 >500 0.001163039065732078 0.6171345532573876 >1k 0.001163039065732078 1.160738181020278 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1757 0.4616625632913887 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1395 0.3665448353964071 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1268 0.3331748037868417 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 929 0.2441004674431987 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 796 0.20915389890719716 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 625 0.1642225965037666 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 496 0.13032705258538918 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 408 0.10720451099765883 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 1.313780772030133E-4 14-15 0.0 0.0 0.0 0.0 2.627561544060266E-4 16-17 0.0 0.0 0.0 0.0 2.627561544060266E-4 18-19 0.0 0.0 0.0 0.0 2.627561544060266E-4 20-21 0.0 0.0 0.0 0.0 2.627561544060266E-4 22-23 0.0 0.0 0.0 3.9413423160903987E-4 2.627561544060266E-4 24-25 0.0 0.0 0.0 7.882684632180797E-4 2.627561544060266E-4 26-27 0.0 0.0 0.0 0.001313780772030133 2.627561544060266E-4 28-29 0.0 0.0 0.0 0.003153073852872319 2.627561544060266E-4 30-31 0.0 0.0 0.0 0.008670953095398877 2.627561544060266E-4 32-33 0.0 0.0 0.0 0.02364805389654239 2.627561544060266E-4 34-35 0.0 0.0 0.0 0.044931302403430545 2.627561544060266E-4 36-37 0.0 0.0 0.0 0.08224267632908631 2.627561544060266E-4 38-39 0.0 0.0 0.0 0.1296701621993741 2.627561544060266E-4 40-41 0.0 0.0 0.0 0.18826478463191804 2.627561544060266E-4 42-43 0.0 0.0 0.0 0.24699078514166498 2.627561544060266E-4 44-45 0.0 0.0 0.0 0.31675254413646503 2.627561544060266E-4 46-47 0.0 0.0 0.0 0.4093740885645894 2.627561544060266E-4 48-49 0.0 0.0 0.0 0.5065938656948192 2.627561544060266E-4 50-51 0.0 0.0 0.0 0.603025374361831 2.627561544060266E-4 52-53 0.0 0.0 0.0 0.7035296034221361 2.627561544060266E-4 54-55 0.0 0.0 0.0 0.8292584233054199 2.627561544060266E-4 56-57 0.0 0.0 0.0 0.9739056863059375 2.627561544060266E-4 58-59 0.0 0.0 0.0 1.1400989539677493 2.627561544060266E-4 60-61 0.0 0.0 0.0 1.3220575908939227 2.627561544060266E-4 62-63 0.0 0.0 0.0 1.5204384874704728 2.627561544060266E-4 64-65 0.0 0.0 0.0 1.7205272990506622 2.627561544060266E-4 66-67 0.0 0.0 0.0 1.957664728402101 2.627561544060266E-4 68-69 0.0 0.0 0.0 2.2177933212640673 2.627561544060266E-4 70-71 0.0 0.0 0.0 2.530210388852833 2.627561544060266E-4 72-73 0.0 0.0 0.0 2.840788163360756 2.627561544060266E-4 74-75 0.0 0.0 0.0 3.1725178082983647 2.627561544060266E-4 76-77 0.0 0.0 0.0 3.525267945588456 2.627561544060266E-4 78-79 0.0 0.0 0.0 3.9308320699141577 2.627561544060266E-4 80-81 0.0 0.0 0.0 4.37042311623544 2.627561544060266E-4 82-83 0.0 0.0 0.0 4.819473384115339 2.627561544060266E-4 84-85 0.0 0.0 0.0 5.263925419293133 2.627561544060266E-4 86-87 0.0 0.0 0.0 5.741221973771681 2.627561544060266E-4 88-89 0.0 0.0 0.0 6.270412868745418 2.627561544060266E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGCTT 30 0.0039333985 31.694107 84-85 CTCCGAA 60 0.003981984 31.610703 2 GTGTCGC 65 0.0059012743 29.167597 9 TGTTCGC 65 0.0059012743 29.167597 8 GCGGCGT 35 0.008359806 27.162796 40-41 ACTATAC 90 9.029249E-4 26.34225 3 GTACATG 2190 0.0 25.141819 1 CTATAGG 115 1.2857762E-4 24.76488 1 GAGTACT 1595 0.0 24.218704 12-13 TACATGG 2175 0.0 24.198536 2 GTACTTT 1665 0.0 23.059685 14-15 ACATGGG 2150 0.0 22.274517 3 GTATATG 150 3.0430823E-5 22.150806 1 GCACCGT 150 3.077303E-5 22.11876 6 AGTACTT 1615 0.0 21.86441 12-13 GTATCAA 4005 0.0 21.688593 1 ACGGACC 55 0.0028620514 21.60962 68-69 ATGGGGA 440 0.0 21.549915 5 GACCATA 110 0.0029101677 21.544247 7 TAGGACA 180 5.239268E-6 21.071028 4 >>END_MODULE