FastQCFastQC Report
Fri 27 May 2016
SRR939273_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939273_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences380581
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17630.46323910021782483No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT14590.3833612292783928No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13710.3602386876906625No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10240.26906230211177123No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT7050.18524308885624874No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT5350.1405745426072242No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC4660.12244436795320837No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT4480.11771475717389991No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC3930.10326316868156844No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG554.915194E-543.2438431
ATCTACG600.00396827731.6329887
CGCGGAA605.184089E-627.8398182-83
CCTATCC908.992198E-426.3608253
GTACATG22400.025.9076961
GTATAGT950.001214833825.0359121
TAATACC950.001232669824.9734124
CCTATAC950.001232669824.9734123
TACATGG23000.024.9625532
CCTACCC1751.4960096E-724.402593
ACATGGG22950.024.189933
GAGTACT16900.024.00550712-13
ATTCGCG601.7056476E-423.75907980-81
GTACTTT16900.023.58435814-15
GAGTGCA1652.4452947E-623.0058089
GTGTAAG1703.1009095E-622.3850481
CATGGGG8850.021.9822444
AATTCGC652.9614763E-421.8997678-79
TCGCGCT652.9614763E-421.8997660-61
CATGGGA13250.020.770344
Produced by FastQC (version 0.11.5)