##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939273_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 380581 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.31603784739648 33.0 31.0 34.0 31.0 34.0 2 32.51832855555059 34.0 31.0 34.0 31.0 34.0 3 32.60467022788841 34.0 31.0 34.0 31.0 34.0 4 36.124196951503095 37.0 35.0 37.0 35.0 37.0 5 36.07102036097441 37.0 35.0 37.0 35.0 37.0 6 36.10962712274128 37.0 35.0 37.0 35.0 37.0 7 36.05084594343911 37.0 35.0 37.0 35.0 37.0 8 36.03083968984264 37.0 35.0 37.0 35.0 37.0 9 37.72995761743229 39.0 38.0 39.0 35.0 39.0 10-11 37.65832503461812 39.0 37.0 39.0 35.0 39.0 12-13 37.58246207771802 39.0 37.0 39.0 35.0 39.0 14-15 38.85584014966591 40.0 38.0 41.0 35.0 41.0 16-17 38.86931954038694 40.0 38.0 41.0 35.0 41.0 18-19 38.99609412976476 40.0 38.5 41.0 35.0 41.0 20-21 38.98521996631466 40.0 38.5 41.0 35.0 41.0 22-23 38.90785929933443 40.0 38.0 41.0 35.0 41.0 24-25 38.83376994647657 40.0 38.0 41.0 35.0 41.0 26-27 38.67373305551249 40.0 38.0 41.0 34.0 41.0 28-29 38.51379995322941 40.0 38.0 41.0 34.0 41.0 30-31 38.30309447923044 40.0 38.0 41.0 34.0 41.0 32-33 38.22144694559108 40.0 38.0 41.0 34.0 41.0 34-35 38.11875527154535 40.0 38.0 41.0 33.5 41.0 36-37 37.95343960943925 40.0 37.0 41.0 33.0 41.0 38-39 37.82507928666959 40.0 37.0 41.0 33.0 41.0 40-41 37.63787866446302 40.0 37.0 41.0 33.0 41.0 42-43 37.42304791883988 40.0 37.0 41.0 32.5 41.0 44-45 37.122792519857796 40.0 36.5 41.0 31.5 41.0 46-47 36.73830932180009 40.0 36.0 41.0 31.0 41.0 48-49 36.37991912365567 39.5 36.0 41.0 30.0 41.0 50-51 36.10611144539533 39.0 35.5 41.0 29.5 41.0 52-53 35.850856979197594 39.0 35.0 41.0 28.5 41.0 54-55 35.60356271069759 39.0 35.0 40.0 28.0 41.0 56-57 35.2901984071722 38.5 35.0 40.0 27.5 41.0 58-59 34.98298128387913 38.0 34.0 40.0 26.5 41.0 60-61 34.634768945375626 38.0 34.0 40.0 26.0 41.0 62-63 34.28831970066819 37.0 34.0 40.0 26.0 41.0 64-65 33.971371403196684 37.0 34.0 39.5 25.5 41.0 66-67 33.558409379343686 36.0 33.0 39.0 24.5 40.5 68-69 33.09291583132106 36.0 33.0 39.0 23.0 40.0 70-71 32.607719513060296 35.0 32.0 38.0 22.0 40.0 72-73 32.11403091588913 35.0 31.5 37.5 21.0 39.5 74-75 31.606517403653886 35.0 31.0 37.0 19.5 39.0 76-77 29.663924368268518 33.0 28.5 35.0 14.5 37.5 78-79 30.60896497723218 34.0 30.5 36.0 17.0 38.0 80-81 30.61808524335161 34.0 31.0 35.5 16.5 37.0 82-83 30.263717841931154 34.0 30.5 35.0 10.0 37.0 84-85 29.822602809914315 34.0 30.0 35.0 7.0 36.0 86-87 29.259436230395107 34.0 29.0 35.0 2.0 36.0 88-89 28.68174974578342 34.0 29.0 35.0 2.0 35.5 90-91 28.196805673430887 33.5 28.5 35.0 2.0 35.0 92-93 27.85631573830538 33.0 27.0 35.0 2.0 35.0 94-95 27.353743355553746 33.0 26.5 35.0 2.0 35.0 96-97 26.63250661488619 33.0 25.5 34.5 2.0 35.0 98-99 25.662292652549652 32.0 24.0 34.0 2.0 35.0 100-101 21.34440894316847 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.09666032323981 1101 2 0.19880261598749627 1101 3 -0.0831583962874447 1101 4 0.03516159886073211 1101 5 0.11268220224549452 1101 6 -0.008489268722193799 1101 7 -0.16713847383137193 1101 8 0.18867597045620244 1101 9 0.33393804045119424 1101 10-11 0.2870528083967798 1101 12-13 0.003304231170289995 1101 14-15 0.3464359797983434 1101 16-17 0.21885719398530767 1101 18-19 0.2559721931592094 1101 20-21 0.3298312019094567 1101 22-23 0.19670539068044235 1101 24-25 0.3362216708588619 1101 26-27 0.28775136530703804 1101 28-29 0.45488055530721283 1101 30-31 0.4365962095422873 1101 32-33 0.5128623804105601 1101 34-35 0.5624311915464446 1101 36-37 0.6788436534026729 1101 38-39 0.6382636679316036 1101 40-41 0.736431086855653 1101 42-43 0.7689385177135648 1101 44-45 1.0260087934997628 1101 46-47 1.0228155240244021 1101 48-49 0.9138615684597227 1101 50-51 1.1127996912615856 1101 52-53 0.7189353128090019 1101 54-55 0.7265741614023824 1101 56-57 0.6711653208030768 1101 58-59 0.636646297217581 1101 60-61 0.8104431437190627 1101 62-63 0.879536471724947 1101 64-65 0.74550498947427 1101 66-67 0.609146150014304 1101 68-69 0.44577310033191253 1101 70-71 0.43374471304386475 1101 72-73 0.47702568889006614 1101 74-75 0.45784640727374537 1101 76-77 0.6035237408745395 1101 78-79 0.6212079581264476 1101 80-81 0.6376870587160539 1101 82-83 0.8351079719007579 1101 84-85 0.33387316479985785 1101 86-87 0.7558979664173826 1101 88-89 0.8518111890495739 1101 90-91 1.0403278757154375 1101 92-93 1.1836571217777312 1101 94-95 1.105560450124191 1101 96-97 0.9906790958096572 1101 98-99 0.8594445438808158 1101 100-101 0.49705120790944335 1102 1 -0.056776432633235174 1102 2 0.17164087166507613 1102 3 0.250779123704028 1102 4 0.20547115567205054 1102 5 0.27376902742231124 1102 6 0.15120259608215747 1102 7 0.23000763271872415 1102 8 0.254745558543064 1102 9 0.20246524662100285 1102 10-11 0.11504619788392745 1102 12-13 0.31663720909970294 1102 14-15 0.3874629194152206 1102 16-17 0.29171499687829794 1102 18-19 0.19702845359920218 1102 20-21 0.3014699693686751 1102 22-23 0.26580479715590144 1102 24-25 0.13567168329799983 1102 26-27 0.11223514111824784 1102 28-29 0.40740303460493266 1102 30-31 0.2467572075164881 1102 32-33 0.495465358695391 1102 34-35 0.2423188839088155 1102 36-37 0.5635595079713838 1102 38-39 0.2732727307314704 1102 40-41 0.5797024022055481 1102 42-43 0.5869228444008456 1102 44-45 0.5946861578243201 1102 46-47 0.40704714075680926 1102 48-49 0.5611720493212289 1102 50-51 0.3807920145369934 1102 52-53 0.1671529621674992 1102 54-55 0.27996915731523586 1102 56-57 0.052455970126025875 1102 58-59 -0.1875176150687281 1102 60-61 -0.4231860633747928 1102 62-63 -0.6505129294194489 1102 64-65 -0.6424095003434047 1102 66-67 -0.8089332472206507 1102 68-69 -0.7827827158531875 1102 70-71 -0.7811324949029625 1102 72-73 -0.8644641643281119 1102 74-75 -0.8867621153121696 1102 76-77 -0.5795314888940908 1102 78-79 -0.6073523006076051 1102 80-81 -0.5353235678610488 1102 82-83 -0.423692717582842 1102 84-85 -0.7922330298904079 1102 86-87 -0.6378318199848643 1102 88-89 -0.5674899091485308 1102 90-91 -0.32574260088121676 1102 92-93 -0.6206930221608573 1102 94-95 -0.6743512320025431 1102 96-97 -0.7575971951950713 1102 98-99 -0.43777975153092186 1102 100-101 -0.34568700156562215 1103 1 0.17743168454710911 1103 2 0.19045889628108625 1103 3 0.3229487910827302 1103 4 0.1360479026967667 1103 5 0.17825483761455985 1103 6 -0.12623611818902702 1103 7 0.10425745471037828 1103 8 0.2212545606791636 1103 9 0.24973917602797968 1103 10-11 0.1963451501776916 1103 12-13 0.2726055744745466 1103 14-15 0.4710378354970075 1103 16-17 0.38888212128070165 1103 18-19 0.38806954809619754 1103 20-21 0.33852113405181683 1103 22-23 0.40228633514796996 1103 24-25 0.34082629625700633 1103 26-27 0.4601651584104829 1103 28-29 0.3235102464857178 1103 30-31 0.5264210260496966 1103 32-33 0.42568608903817307 1103 34-35 0.6645292389083082 1103 36-37 0.6440319929678253 1103 38-39 0.6899012527536499 1103 40-41 0.46873963137856833 1103 42-43 0.2444093666264564 1103 44-45 0.48311307268547665 1103 46-47 0.4994461845977227 1103 48-49 0.6053689774058597 1103 50-51 0.5167776721506598 1103 52-53 0.3772409491474633 1103 54-55 0.5854060406434769 1103 56-57 0.9037681458965565 1103 58-59 0.7395752544257235 1103 60-61 1.2142999502555583 1103 62-63 1.313859972625103 1103 64-65 0.8975788019656079 1103 66-67 0.8793995775174253 1103 68-69 0.6677818248852958 1103 70-71 0.9756191071721076 1103 72-73 1.284808544450243 1103 74-75 0.8849167576407346 1103 76-77 1.154676628286257 1103 78-79 0.9027306004197584 1103 80-81 1.206894924662599 1103 82-83 1.3586157963018088 1103 84-85 1.2433559234205482 1103 86-87 1.1320923993214116 1103 88-89 1.2412655582505145 1103 90-91 1.5222382759370099 1103 92-93 1.0556623842067552 1103 94-95 1.2035731907418388 1103 96-97 1.422267518465798 1103 98-99 1.0283096595157666 1103 100-101 0.2710610403426301 1104 1 0.051206553486579764 1104 2 -0.09474835844849139 1104 3 -0.08425058373411076 1104 4 -0.04074542868286812 1104 5 -0.03228861587115972 1104 6 -0.06682743726037188 1104 7 0.18951192379311976 1104 8 0.18638920298973716 1104 9 -0.00801049021538347 1104 10-11 -0.003119211126076493 1104 12-13 0.1524816774995159 1104 14-15 0.13035778600333714 1104 16-17 0.19788378192347267 1104 18-19 0.183887821678951 1104 20-21 0.1518046481021571 1104 22-23 0.07283597534203778 1104 24-25 0.17769663587473872 1104 26-27 0.07811963726171456 1104 28-29 -0.009098590738723544 1104 30-31 0.11346231418749397 1104 32-33 -0.059921673652716834 1104 34-35 0.02969969900403413 1104 36-37 0.06625448653690569 1104 38-39 0.09238326245701245 1104 40-41 0.13758385657475003 1104 42-43 0.12556758355198383 1104 44-45 -0.18972809171815896 1104 46-47 -0.36029055468136306 1104 48-49 -0.1348218337041729 1104 50-51 -0.18876350202145886 1104 52-53 -0.04356511382671613 1104 54-55 0.19343736492468366 1104 56-57 -0.17650439488688363 1104 58-59 -0.05789959172060577 1104 60-61 -0.13885120079780933 1104 62-63 0.20939414604830375 1104 64-65 0.2214000712176727 1104 66-67 0.272923070728325 1104 68-69 -0.07955626244497438 1104 70-71 0.1574554198939282 1104 72-73 -0.261770857067134 1104 74-75 -0.20778211247095157 1104 76-77 0.5180643395297828 1104 78-79 0.43864200024392375 1104 80-81 0.5981635254896673 1104 82-83 0.4336906880344138 1104 84-85 0.37565786734943174 1104 86-87 0.2462005364709654 1104 88-89 0.5163176568471108 1104 90-91 0.8341134997981676 1104 92-93 0.44987781190367215 1104 94-95 0.2580528560083515 1104 96-97 -0.4144115216175628 1104 98-99 -0.7815670947416642 1104 100-101 -0.1886846533827473 1105 1 0.22210181523519168 1105 2 0.2137308219620806 1105 3 0.08974142216444392 1105 4 0.10923322784241662 1105 5 0.13306510371438662 1105 6 0.21322223482624736 1105 7 0.2328120130476492 1105 8 0.17345893794259126 1105 9 0.19507849267102984 1105 10-11 0.18265439281665152 1105 12-13 0.2904885850240575 1105 14-15 0.13820442343591566 1105 16-17 0.21672812978329858 1105 18-19 0.40571637536748995 1105 20-21 0.2837344862798119 1105 22-23 0.12939230271212665 1105 24-25 0.12387227326850336 1105 26-27 0.37542259818375356 1105 28-29 0.5111893521383735 1105 30-31 0.2783852836469194 1105 32-33 0.18561365949951636 1105 34-35 0.2250157764614471 1105 36-37 0.16800853209552002 1105 38-39 -0.1907592731477834 1105 40-41 0.18503234118804102 1105 42-43 0.524042495267615 1105 44-45 0.32162275619987923 1105 46-47 0.6376488298504412 1105 48-49 0.4355689459147527 1105 50-51 0.13570776190030642 1105 52-53 0.37244249221154746 1105 54-55 0.5548832883210082 1105 56-57 0.5283075885040134 1105 58-59 0.511823719234421 1105 60-61 0.3201848502712963 1105 62-63 0.7139150820566869 1105 64-65 0.42374397280485 1105 66-67 0.4580486091659779 1105 68-69 0.6468087497790123 1105 70-71 0.5365504565658981 1105 72-73 0.7483575918443535 1105 74-75 0.7499655688515681 1105 76-77 0.5605339735862103 1105 78-79 0.38191104444432256 1105 80-81 0.611280060861624 1105 82-83 0.6945195909798123 1105 84-85 0.7126402880875311 1105 86-87 0.6963460601622238 1105 88-89 0.614738239718001 1105 90-91 0.6050126421785471 1105 92-93 0.476312675499468 1105 94-95 0.9046320791058768 1105 96-97 1.0239601652930688 1105 98-99 1.0016957404479037 1105 100-101 1.640412323605105 1106 1 0.0488575418663828 1106 2 0.16006087215265552 1106 3 0.1487776122093578 1106 4 0.06105027754240666 1106 5 -0.03377138068125163 1106 6 0.04432529531909779 1106 7 0.11606043751649509 1106 8 0.16361705711689467 1106 9 0.07905902023811251 1106 10-11 0.15851385921160954 1106 12-13 0.19626616153637855 1106 14-15 0.21819989496313497 1106 16-17 0.17602470701262973 1106 18-19 0.22490577874120277 1106 20-21 0.09132345190116098 1106 22-23 0.2607217868436109 1106 24-25 0.23131028979743462 1106 26-27 0.2615805664089663 1106 28-29 0.317359396290982 1106 30-31 0.048946436897992385 1106 32-33 0.17290751964518591 1106 34-35 0.49432405224543885 1106 36-37 0.39922103068037273 1106 38-39 0.6106407796844024 1106 40-41 0.5742658971712942 1106 42-43 0.6539039994753182 1106 44-45 0.786036507662196 1106 46-47 0.5271012620176947 1106 48-49 0.8275120381069101 1106 50-51 0.820722290750453 1106 52-53 0.9815560436663162 1106 54-55 0.9171007304886416 1106 56-57 0.6007140944099021 1106 58-59 0.7367491881200365 1106 60-61 0.8727377767968321 1106 62-63 0.9925671066613901 1106 64-65 0.9882860309957238 1106 66-67 1.0054088869123134 1106 68-69 1.0853271844369274 1106 70-71 0.8008660911181522 1106 72-73 1.3031898496654009 1106 74-75 1.1654453982790933 1106 76-77 1.1197663974512615 1106 78-79 1.5350451249756674 1106 80-81 1.3387226058633246 1106 82-83 1.077635934992795 1106 84-85 1.1596307405574358 1106 86-87 1.2514877636227304 1106 88-89 1.6869409643491196 1106 90-91 1.1249266011958703 1106 92-93 1.2704671718955574 1106 94-95 0.7729780544256961 1106 96-97 0.9001102086909327 1106 98-99 1.4562449687206716 1106 100-101 0.900893851209922 1107 1 0.031472679715321306 1107 2 -0.030812974311032804 1107 3 0.06657893423569305 1107 4 -0.046344582522763744 1107 5 0.122764306117908 1107 6 0.11344756477367213 1107 7 0.11904542625985215 1107 8 0.11641827125207982 1107 9 0.16645085935081028 1107 10-11 0.14575393142675352 1107 12-13 0.12553991017875177 1107 14-15 0.26393495743212014 1107 16-17 0.26758474125009 1107 18-19 0.15038535494473138 1107 20-21 0.12213357065336083 1107 22-23 0.1840830999509464 1107 24-25 0.05598715577502844 1107 26-27 -0.036008425815673206 1107 28-29 -0.11350394876652103 1107 30-31 0.17806338372204067 1107 32-33 0.26136028849197857 1107 34-35 0.08446711592279854 1107 36-37 0.46077293711716294 1107 38-39 0.482415733111381 1107 40-41 0.34527770046413053 1107 42-43 0.32363117869692104 1107 44-45 0.2635555864155563 1107 46-47 0.4657399632958388 1107 48-49 0.6745295668239422 1107 50-51 0.9426464936146033 1107 52-53 1.078776767400214 1107 54-55 0.9077177959929585 1107 56-57 0.7866573148890765 1107 58-59 1.0122636491317678 1107 60-61 0.8810453960705047 1107 62-63 1.022384626747126 1107 64-65 1.1473499672967833 1107 66-67 1.3673801303219548 1107 68-69 1.0537546348249762 1107 70-71 0.9842810620954197 1107 72-73 0.781942484007871 1107 74-75 0.7346324024293089 1107 76-77 0.5905278932542934 1107 78-79 0.8080493183453896 1107 80-81 0.7364633649855179 1107 82-83 1.0753522387855305 1107 84-85 0.9663796922150034 1107 86-87 0.9087535139338137 1107 88-89 1.252110820997732 1107 90-91 0.8803157646078184 1107 92-93 0.9319190991913011 1107 94-95 1.07072528701746 1107 96-97 1.27622598309771 1107 98-99 1.5630212528052887 1107 100-101 1.1208479615034577 1108 1 -0.16566878594303347 1108 2 -0.07367069695338557 1108 3 -0.07351359948364689 1108 4 0.10055269693177848 1108 5 -0.04207152770019462 1108 6 0.08045198853481139 1108 7 0.006124526355904436 1108 8 -0.0480117129197879 1108 9 -0.09971886697124432 1108 10-11 -0.05760041589937259 1108 12-13 0.025278137156924174 1108 14-15 -0.2048078556470614 1108 16-17 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-0.9903024912858385 2316 54-55 -1.0724438723531122 2316 56-57 -1.2003602270172067 2316 58-59 -0.975794938942407 2316 60-61 -1.0742480731740969 2316 62-63 -0.9381864455304196 2316 64-65 -0.8124945994999706 2316 66-67 -0.5888012900658453 2316 68-69 -0.6708329828494328 2316 70-71 -0.6760762338567403 2316 72-73 -0.4222473092860035 2316 74-75 -0.4080243251227884 2316 76-77 -1.0835411498182914 2316 78-79 -1.1214128449151417 2316 80-81 -1.0607998526822016 2316 82-83 -0.881608574455246 2316 84-85 -0.9289200121724228 2316 86-87 -1.1839413481971555 2316 88-89 -1.7272631038576627 2316 90-91 -1.4864498107232826 2316 92-93 -1.4862891879815656 2316 94-95 -1.4512285395230968 2316 96-97 -0.724884651717769 2316 98-99 -0.888648039673015 2316 100-101 -0.07461819892899513 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 1.0 5 12.0 6 27.0 7 52.0 8 110.0 9 199.0 10 361.0 11 561.0 12 860.0 13 1197.0 14 1466.0 15 2063.0 16 2904.0 17 4142.0 18 3805.0 19 2387.0 20 2101.0 21 2153.0 22 2293.0 23 2543.0 24 2903.0 25 3432.0 26 4037.0 27 4996.0 28 5981.0 29 7295.0 30 8888.0 31 10876.0 32 13690.0 33 17342.0 34 24186.0 35 34889.0 36 54968.0 37 88677.0 38 65202.0 39 3980.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.918206110692395 19.778320060295258 15.44749727248291 21.855976556529438 2 15.406707113597367 21.364440158599614 39.48883417721852 23.7400185505845 3 19.57796106479304 25.576421313728222 30.13734264190803 24.70827497957071 4 12.98751119998108 17.35005163158434 37.23228432317956 32.430152845255016 5 13.508819410322639 38.18608916367344 33.50062141830517 14.804470007698756 6 30.79554680869513 36.85811955930538 17.30512032918091 15.041213302818585 7 27.935183311831118 33.81776809667325 20.51547502371374 17.73157356778189 8 26.50736636878877 34.114419794997644 19.789742525244296 19.58847131096928 9 26.64767815524159 17.506391543455926 20.68889408562172 35.15703621568076 10-11 25.10950362734871 27.007654086777848 26.589871801272263 21.292970484601177 12-13 26.326984268789033 24.459444901348203 27.562069572574565 21.6515012572882 14-15 23.023653619776336 25.553634490876988 26.79504225174472 24.62766963760195 16-17 21.737593870376113 29.098634182743087 27.16000399392503 22.003767952955766 18-19 21.588965292539562 27.475754175852185 29.940801038412324 20.994479493195932 20-21 22.761908765808066 26.13149368991095 29.748726289541516 21.357871254739464 22-23 22.249665642793516 26.165257855752127 29.803379569657974 21.781696931796386 24-25 22.314435034854604 26.42013132552597 29.936596939941822 21.3288366996776 26-27 21.926606950951307 26.39989910163671 30.260312522170054 21.413181425241934 28-29 21.389796127499796 27.040235849924194 30.19239005625609 21.377577966319915 30-31 21.77184357600616 26.4780690575725 29.90546033564471 21.84462703077663 32-33 21.654785709218274 26.663443524505954 29.831625856256615 21.850144910019154 34-35 22.449977008474022 26.506995992905473 29.54949747093214 21.493529527688366 36-37 22.384586782777326 26.211613781396455 29.84799891737495 21.555800518451267 38-39 21.753325240294924 26.75025093674292 29.927556795787414 21.56886702717474 40-41 21.961720476444484 26.596228279234513 29.84058081451721 21.601470429803793 42-43 21.91197896891451 26.852865170089295 29.065806224455077 22.169349636541117 44-45 21.936091608509205 26.749716219624993 29.107720928277136 22.206471243588666 46-47 22.437171636486894 27.20252351342226 28.450222751822547 21.9100820982683 48-49 22.38097114929844 27.11033159913816 28.375899942193495 22.13279730936991 50-51 22.02553464308518 27.35055086827771 28.472125513359835 22.151788975277274 52-53 22.477606606740746 27.637874723120703 27.860823320134216 22.023695350004335 54-55 21.755027182124174 27.260951019625256 28.23249190054154 22.75152989770903 56-57 22.179100791162938 27.586125351109757 27.952672112754513 22.282101744972792 58-59 22.553957968752464 27.013274685080958 28.12383399811863 22.308933348047947 60-61 22.367366565154672 27.28237764038478 28.566605093817575 21.78365070064297 62-63 22.034613095590004 27.233803275802494 28.531958584102885 22.199625044504618 64-65 21.732590082781435 27.351769205201006 28.318975880267118 22.59666483175044 66-67 21.855383507760546 27.408501257294805 27.90918574067408 22.826929494270573 68-69 21.68871453214688 27.30103383446299 28.28008255961069 22.73016907377944 70-71 21.99363866514443 27.396963323134 27.98172791301709 22.62767009870448 72-73 22.028950473092458 27.039053447229367 28.178364132733897 22.753631946944278 74-75 21.497657528883472 27.87593179901256 27.72221944868504 22.90419122341893 76-77 21.836114546740585 27.073331041450153 28.087818640913277 23.00273577089598 78-79 21.697906224832856 27.19897630067752 28.140826973531226 22.962290500958403 80-81 21.881412892393474 27.591498261868036 27.678733305130837 22.848355540607653 82-83 21.85172144694559 27.268308191948627 27.963560976506972 22.91640938459881 84-85 21.533523743959893 27.308772639727152 27.803805234628108 23.353898381684846 86-87 21.556267220801082 27.319045959400007 27.906279386331207 23.218407433467704 88-89 21.56619690078476 27.134155588464516 27.647922185987202 23.65172532476352 90-91 21.41014628696697 27.023961341455703 27.97672371998904 23.589168651588288 92-93 21.76671983099524 26.99031218058705 27.831000496609132 23.41196749180858 94-95 21.327260162751163 26.827009230623705 27.86318812552387 23.982542481101262 96-97 21.073306339517735 26.73044634387949 28.13527212341131 24.06097519319146 98-99 21.054021422537414 26.863042115925538 27.63672862902855 24.446207832508495 100-101 21.104553850843057 26.036906923398295 28.482522682544232 24.37601654321442 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 110.0 1 70.5 2 39.0 3 57.0 4 57.5 5 63.5 6 116.0 7 247.0 8 408.5 9 466.5 10 449.5 11 435.5 12 472.5 13 552.5 14 570.5 15 588.0 16 636.5 17 688.5 18 740.5 19 778.0 20 842.0 21 950.5 22 1064.0 23 1208.5 24 1442.0 25 1712.5 26 2139.0 27 2680.0 28 3161.5 29 3743.5 30 4435.0 31 5080.5 32 5915.5 33 6889.5 34 7791.5 35 8396.0 36 9114.0 37 10063.0 38 10515.0 39 10659.0 40 10914.5 41 11504.0 42 12009.0 43 12427.5 44 12884.5 45 13421.0 46 13691.5 47 14613.5 48 17802.0 49 18058.5 50 15488.5 51 14093.0 52 13016.5 53 12658.0 54 12414.5 55 11301.0 56 9910.5 57 8757.5 58 7651.0 59 6684.5 60 5659.5 61 4612.0 62 3776.5 63 2946.0 64 2228.5 65 1736.5 66 1262.5 67 1028.0 68 775.0 69 470.5 70 341.0 71 277.5 72 236.5 73 182.0 74 138.5 75 112.0 76 82.0 77 53.0 78 23.0 79 15.5 80 15.5 81 5.5 82 2.0 83 2.0 84 2.0 85 1.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29297311216271965 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0013137807720301328 16-17 7.882684632180796E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0015765369264361593 36-37 0.0064375257829476515 38-39 0.0018392930808421861 40-41 0.0031530738528723185 42-43 3.941342316090398E-4 44-45 0.0013137807720301328 46-47 1.313780772030133E-4 48-49 2.627561544060266E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 5.255123088120532E-4 58-59 0.0018392930808421861 60-61 5.255123088120532E-4 62-63 6.568903860150664E-4 64-65 1.313780772030133E-4 66-67 5.255123088120532E-4 68-69 0.001970671158045199 70-71 1.313780772030133E-4 72-73 0.0 74-75 0.0 76-77 0.25763240939510906 78-79 1.313780772030133E-4 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.10733588907486184 88-89 0.37324511733376076 90-91 0.276682230589546 92-93 0.0 94-95 0.0 96-97 0.0 98-99 1.313780772030133E-4 100-101 5.255123088120532E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 380581.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.83136438223609 #Duplication Level Percentage of deduplicated Percentage of total 1 85.1657259166711 56.917416616146944 2 9.060373614019875 12.110342608755191 3 2.3499838173284284 4.71157874364703 4 0.9822559581569927 2.625820234248497 5 0.5525514095482715 1.846388229571935 6 0.38025078272943674 1.5247607170332884 7 0.25985331482908214 1.215644609849201 8 0.1862836682295493 0.9959673367926868 9 0.13501582576072702 0.8120962663905282 >10 0.8518922329925418 10.368676372609547 >50 0.05028042925444877 2.2999744674404208 >100 0.023176135358334418 2.771205396345601 >500 7.856317070621837E-4 0.325530001086448 >1k 0.0015712634141243674 1.4745984000827246 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1763 0.46323910021782483 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1459 0.3833612292783928 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1371 0.3602386876906625 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1024 0.26906230211177123 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 705 0.18524308885624874 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 535 0.1405745426072242 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 466 0.12244436795320837 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 448 0.11771475717389991 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 393 0.10326316868156844 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 3.9413423160903987E-4 0.0 24-25 0.0 0.0 0.0 7.882684632180797E-4 0.0 26-27 0.0 0.0 0.0 0.0015765369264361595 0.0 28-29 0.0 0.0 0.0 0.0034158300072783454 0.0 30-31 0.0 0.0 0.0 0.00919646540421093 1.313780772030133E-4 32-33 0.0 0.0 0.0 0.024173566205354446 2.627561544060266E-4 34-35 0.0 0.0 0.0 0.045456814712242596 2.627561544060266E-4 36-37 0.0 0.0 0.0 0.08342507902391344 2.627561544060266E-4 38-39 0.0 0.0 0.0 0.13032705258538918 2.627561544060266E-4 40-41 0.0 0.0 0.0 0.1889216750179331 2.627561544060266E-4 42-43 0.0 0.0 0.0 0.24751629745047704 2.627561544060266E-4 44-45 0.0 0.0 0.0 0.31793494683129214 2.627561544060266E-4 46-47 0.0 0.0 0.0 0.41200165010864964 2.627561544060266E-4 48-49 0.0 0.0 0.0 0.5092214272388795 2.627561544060266E-4 50-51 0.0 0.0 0.0 0.6056529359058913 2.627561544060266E-4 52-53 0.0 0.0 0.0 0.7074709457382266 2.627561544060266E-4 54-55 0.0 0.0 0.0 0.8338566560075253 2.627561544060266E-4 56-57 0.0 0.0 0.0 0.9788980532396521 2.627561544060266E-4 58-59 0.0 0.0 0.0 1.1445658085926518 2.627561544060266E-4 60-61 0.0 0.0 0.0 1.326261689364419 2.627561544060266E-4 62-63 0.0 0.0 0.0 1.5258249886357964 2.627561544060266E-4 64-65 0.0 0.0 0.0 1.7274903371424217 2.627561544060266E-4 66-67 0.0 0.0 0.0 1.9656787911114848 2.627561544060266E-4 68-69 0.0 0.0 0.0 2.227383920899887 2.627561544060266E-4 70-71 0.0 0.0 0.0 2.540852013106277 2.627561544060266E-4 72-73 0.0 0.0 0.0 2.852612190309028 2.627561544060266E-4 74-75 0.0 0.0 0.0 3.1832908106290123 2.627561544060266E-4 76-77 0.0 0.0 0.0 3.537354728691133 2.627561544060266E-4 78-79 0.0 0.0 0.0 3.9422619626308197 2.627561544060266E-4 80-81 0.0 0.0 0.0 4.3829040335697265 2.627561544060266E-4 82-83 0.0 0.0 0.0 4.833268082221656 2.627561544060266E-4 84-85 0.0 0.0 0.0 5.280873191252322 2.627561544060266E-4 86-87 0.0 0.0 0.0 5.758301123808073 2.627561544060266E-4 88-89 0.0 0.0 0.0 6.283419298388516 2.627561544060266E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACTAG 55 4.915194E-5 43.243843 1 ATCTACG 60 0.003968277 31.632988 7 CGCGGAA 60 5.184089E-6 27.83981 82-83 CCTATCC 90 8.992198E-4 26.360825 3 GTACATG 2240 0.0 25.907696 1 GTATAGT 95 0.0012148338 25.035912 1 TAATACC 95 0.0012326698 24.973412 4 CCTATAC 95 0.0012326698 24.973412 3 TACATGG 2300 0.0 24.962553 2 CCTACCC 175 1.4960096E-7 24.40259 3 ACATGGG 2295 0.0 24.18993 3 GAGTACT 1690 0.0 24.005507 12-13 ATTCGCG 60 1.7056476E-4 23.759079 80-81 GTACTTT 1690 0.0 23.584358 14-15 GAGTGCA 165 2.4452947E-6 23.005808 9 GTGTAAG 170 3.1009095E-6 22.385048 1 CATGGGG 885 0.0 21.982244 4 AATTCGC 65 2.9614763E-4 21.89976 78-79 TCGCGCT 65 2.9614763E-4 21.89976 60-61 CATGGGA 1325 0.0 20.77034 4 >>END_MODULE